Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,348,894 T→A 100% V384E (GTG→GAG)  glpB → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,348,8940TA99.0% 669.7 / ‑2.6 205V384E (GTG→GAG) glpBsn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (98/105);  total (98/107)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  >  CP009273/2348749‑2349037
                                                                                                                                                 |                                                                                                                                               
cTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGc                                                                                                                                               <  2:2122219/148‑1 (MQ=255)
  aCAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTg                                                                                                                                             <  1:1100938/148‑1 (MQ=255)
   cAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTgg                                                                                                                                            >  2:6874/1‑148 (MQ=255)
     aaCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGc                                                                                                                                          >  2:2603907/1‑148 (MQ=255)
     aaCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATCAGCCGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGc                                                                                                                                          <  2:2902056/148‑1 (MQ=255)
       ccGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCgg                                                                                                                                        <  1:757332/148‑1 (MQ=255)
          ccACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAtt                                                                                                                                     >  1:3486042/1‑148 (MQ=255)
          ccACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAtt                                                                                                                                     >  2:1215698/1‑148 (MQ=255)
           cACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAttt                                                                                                                                    <  1:2153191/148‑1 (MQ=255)
           cACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAttt                                                                                                                                    <  1:2719586/148‑1 (MQ=255)
           cACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAttt                                                                                                                                    <  1:2719608/148‑1 (MQ=255)
            aCGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTg                                                                                                                                   <  1:1123345/148‑1 (MQ=255)
            aCGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTg                                                                                                                                   <  1:1403387/148‑1 (MQ=255)
               cgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATc                                                                                                                                <  2:1753572/148‑1 (MQ=255)
                ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATcc                                                                                                                               <  1:1619480/148‑1 (MQ=255)
                ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATcc                                                                                                                               <  1:1475526/148‑1 (MQ=255)
                 gggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATccc                                                                                                                              >  1:1869783/1‑148 (MQ=255)
                         ggTATAAGGGAGAGTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCa                                                                                                                      <  1:1879031/148‑1 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATTAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   >  1:1337510/1‑148 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATTAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   >  1:2508580/1‑148 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   <  1:1432381/148‑1 (MQ=255)
                            ataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAggg                                                                                                                   >  1:591381/1‑148 (MQ=255)
                             taAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGa                                                                                                                  <  2:2346394/148‑1 (MQ=255)
                             taAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTAGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGa                                                                                                                  <  1:981917/148‑1 (MQ=255)
                              aaGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                 <  1:2530308/148‑1 (MQ=255)
                              aaGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                 >  2:355255/1‑148 (MQ=255)
                              aaGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                 <  1:770705/148‑1 (MQ=255)
                              aaGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                 <  1:1133117/148‑1 (MQ=255)
                               aGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATg                                                                                                                <  2:2009615/148‑1 (MQ=255)
                                gggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgc                                                                                                               >  2:1874747/1‑148 (MQ=255)
                                gggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgc                                                                                                               >  2:885348/1‑148 (MQ=255)
                                gggAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgc                                                                                                               >  2:848396/1‑148 (MQ=255)
                                  gagaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcgg                                                                                                             <  1:1897376/148‑1 (MQ=255)
                                    gaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcg                                                                                                           >  2:794130/1‑148 (MQ=255)
                                     aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcgg                                                                                                          >  2:56285/1‑148 (MQ=255)
                                      tttttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcggc                                                                                                         >  2:839466/1‑148 (MQ=255)
                                         ttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgt                                                                                                      <  2:458852/148‑1 (MQ=255)
                                          tttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgtg                                                                                                     <  2:1008436/148‑1 (MQ=255)
                                           ttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgtgt                                                                                                    >  1:1818936/1‑148 (MQ=255)
                                           ttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgtgt                                                                                                    <  1:3042939/148‑1 (MQ=255)
                                           ttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgtgt                                                                                                    <  1:1946242/148‑1 (MQ=255)
                                                ccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTtgtg                                                                                               >  1:909815/1‑148 (MQ=255)
                                                ccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTtgtg                                                                                               >  2:1348495/1‑148 (MQ=255)
                                                ccgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTtgtg                                                                                               <  2:1361542/148‑1 (MQ=255)
                                                 cgcAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGc                                                                                              <  2:2464646/148‑1 (MQ=255)
                                                    aaCCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGt                                                                                           >  2:297684/1‑148 (MQ=255)
                                                         tGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTg                                                                                      <  2:1486819/148‑1 (MQ=255)
                                                             agcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCttt                                                                                  <  2:2824307/148‑1 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                                >  2:1044000/1‑148 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                                >  1:1473313/1‑148 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                                >  2:910630/1‑148 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                                <  1:3215872/148‑1 (MQ=255)
                                                                 gTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACAt                                                                              >  2:2200196/1‑148 (MQ=255)
                                                                   tCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGc                                                                            >  2:2046406/1‑148 (MQ=255)
                                                                     ggTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTGTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCg                                                                          <  2:3483796/148‑1 (MQ=255)
                                                                       tgtAACCACTGATGAGACTCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCt                                                                        <  2:750954/148‑1 (MQ=255)
                                                                           aCCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAAc                                                                    >  2:2660737/1‑148 (MQ=255)
                                                                            ccACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACa                                                                   >  2:1058842/1‑148 (MQ=255)
                                                                             cACTGATGAGACGCTACGCCCGTAACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGTTGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                  <  1:1907243/148‑1 (MQ=255)
                                                                                 gatgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTg                                                              <  2:1943798/148‑1 (MQ=255)
                                                                                  atgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGc                                                             >  1:507141/1‑148 (MQ=255)
                                                                                   tgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGcc                                                            <  1:856633/148‑1 (MQ=255)
                                                                                     agaCGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCa                                                          >  1:1707671/1‑148 (MQ=255)
                                                                                          cTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAAcgcg                                                     >  2:1584081/1‑148 (MQ=255)
                                                                                          cTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAAcgcg                                                     <  1:3312724/148‑1 (MQ=255)
                                                                                            aCGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCa                                                   <  2:2920665/148‑1 (MQ=255)
                                                                                             cGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCag                                                  >  2:1796728/1‑148 (MQ=255)
                                                                                             cGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCag                                                  <  1:1796728/148‑1 (MQ=255)
                                                                                              gCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCagg                                                 >  1:3264864/1‑148 (MQ=255)
                                                                                                 cGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCaggagg                                              >  1:1563596/1‑148 (MQ=255)
                                                                                                   tCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGcc                                            >  1:2287556/1‑148 (MQ=255)
                                                                                                    cacaGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCca                                           <  1:1839180/148‑1 (MQ=255)
                                                                                                       aggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaaca                                        <  1:1430649/148‑1 (MQ=255)
                                                                                                        ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaacaa                                       <  1:2694145/148‑1 (MQ=255)
                                                                                                         gcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGCGGCCAACaat                                      >  1:119039/1‑148 (MQ=255)
                                                                                                         gcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaat                                      <  2:1112148/148‑1 (MQ=255)
                                                                                                           aggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatga                                    <  2:2140969/148‑1 (MQ=255)
                                                                                                                 aaCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGaca                              >  2:3415286/1‑148 (MQ=255)
                                                                                                                   ccATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAcc                            >  1:1331784/1‑148 (MQ=255)
                                                                                                                     aTTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAg                          <  1:900383/148‑1 (MQ=255)
                                                                                                                      ttGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGc                         <  1:209936/148‑1 (MQ=255)
                                                                                                                        gAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCtt                       <  1:248189/148‑1 (MQ=255)
                                                                                                                         aaaaCCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTc                      >  2:2133668/1‑148 (MQ=255)
                                                                                                                             ccTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                  >  1:2591912/1‑148 (MQ=255)
                                                                                                                             ccTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                  <  2:1850371/148‑1 (MQ=255)
                                                                                                                             ccTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                  <  2:3513843/148‑1 (MQ=255)
                                                                                                                             ccTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                  <  2:440109/148‑1 (MQ=255)
                                                                                                                             ccTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                  <  1:627624/148‑1 (MQ=255)
                                                                                                                              cTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaaa                 >  2:1725837/1‑148 (MQ=255)
                                                                                                                              cTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaaa                 >  1:3190176/1‑148 (MQ=255)
                                                                                                                               tGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAAc                >  1:2632240/1‑148 (MQ=255)
                                                                                                                                 tttGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTg              <  2:31012/148‑1 (MQ=255)
                                                                                                                                   tGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCa            <  1:567021/148‑1 (MQ=255)
                                                                                                                                    gCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCAt           <  2:621061/148‑1 (MQ=255)
                                                                                                                                    gCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCAt           <  1:505661/148‑1 (MQ=255)
                                                                                                                                       aTCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTaa        <  2:1223057/148‑1 (MQ=255)
                                                                                                                                        gcggttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGAGTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAg       <  2:2499364/147‑1 (MQ=255)
                                                                                                                                           gttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGc    <  1:744595/148‑1 (MQ=255)
                                                                                                                                            ttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCa   <  2:1337510/148‑1 (MQ=255)
                                                                                                                                             tcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAc  >  2:2561131/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC  >  CP009273/2348749‑2349037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTG  >  CP009273/2348745‑2349043
                                                                                                                                                     |                                                                                                                                                     
TGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGA                                                                                                                                                       <  SRR19973065.1486363/150‑1 (MQ=60)
 GTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAG                                                                                                                                                      <  SRR19973065.2717687/150‑1 (MQ=60)
 GTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAG                                                                                                                                                      <  SRR19973065.2717622/150‑1 (MQ=60)
      ACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGG                                                                                                                                                 <  SRR19973065.1108173/150‑1 (MQ=60)
       CAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGC                                                                                                                                                >  SRR19973065.1862565/1‑150 (MQ=60)
       CAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGC                                                                                                                                                >  SRR19973065.1862543/1‑150 (MQ=60)
          ACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGA                                                                                                                                             >  SRR19973065.3384393/1‑150 (MQ=60)
           CCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAT                                                                                                                                            <  SRR19973065.1191588/150‑1 (MQ=60)
           CCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAT                                                                                                                                            <  SRR19973065.762380/150‑1 (MQ=60)
           CCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAT                                                                                                                                            <  SRR19973065.762290/150‑1 (MQ=60)
           CCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAT                                                                                                                                            <  SRR19973065.761842/150‑1 (MQ=60)
            CGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATT                                                                                                                                           >  SRR19973065.3507245/1‑150 (MQ=60)
              CCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTG                                                                                                                                         >  SRR19973065.2629511/1‑150 (MQ=60)
              CCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTG                                                                                                                                         <  SRR19973065.1904586/150‑1 (MQ=60)
               CACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGA                                                                                                                                        <  SRR19973065.2167039/150‑1 (MQ=60)
               CACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGA                                                                                                                                        <  SRR19973065.2736501/150‑1 (MQ=60)
               CACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGA                                                                                                                                        <  SRR19973065.2736522/150‑1 (MQ=60)
               CACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGA                                                                                                                                        <  SRR19973065.2736545/150‑1 (MQ=60)
               CACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGA                                                                                                                                        <  SRR19973065.2736523/150‑1 (MQ=60)
                ACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGAT                                                                                                                                       <  SRR19973065.1412571/150‑1 (MQ=60)
                ACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGAT                                                                                                                                       <  SRR19973065.1412586/150‑1 (MQ=60)
                ACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGAT                                                                                                                                       <  SRR19973065.1130664/150‑1 (MQ=60)
                ACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGAT                                                                                                                                       <  SRR19973065.3230486/150‑1 (MQ=60)
                 CGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATC                                                                                                                                      >  SRR19973065.1612388/1‑150 (MQ=60)
                   CGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCC                                                                                                                                    >  SRR19973065.1882087/1‑150 (MQ=60)
                    GGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCA                                                                                                                                   <  SRR19973065.1484739/150‑1 (MQ=60)
                    GGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCA                                                                                                                                   <  SRR19973065.1630340/150‑1 (MQ=60)
                    GGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCA                                                                                                                                   <  SRR19973065.1484963/150‑1 (MQ=60)
                        TGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGC                                                                                                                               >  SRR19973065.1003723/1‑150 (MQ=60)
                         GAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCC                                                                                                                              >  SRR19973065.2154741/1‑150 (MQ=60)
                          AATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCC                                                                                                                             <  SRR19973065.950587/150‑1 (MQ=60)
                          AATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCC                                                                                                                             >  SRR19973065.3269749/1‑150 (MQ=60)
                            TGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAG                                                                                                                           <  SRR19973065.1417770/150‑1 (MQ=60)
                             GGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGG                                                                                                                          <  SRR19973065.1891353/150‑1 (MQ=60)
                             GGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGG                                                                                                                          >  SRR19973065.2701639/1‑150 (MQ=60)
                             GGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGG                                                                                                                          <  SRR19973065.430714/150‑1 (MQ=60)
                             GGTATAAGGGAGAGTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGG                                                                                                                          <  SRR19973065.1891402/150‑1 (MQ=60)
                              GTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATTAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGG                                                                                                                         >  SRR19973065.2524341/1‑150 (MQ=60)
                              GTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATTAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGG                                                                                                                         >  SRR19973065.1346369/1‑150 (MQ=60)
                              GTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGG                                                                                                                         >  SRR19973065.595042/1‑150 (MQ=60)
                              GTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGG                                                                                                                         <  SRR19973065.3126679/150‑1 (MQ=60)
                              GTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGG                                                                                                                         >  SRR19973065.1119417/1‑150 (MQ=60)
                                ATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAT                                                                                                                       <  SRR19973065.1441691/150‑1 (MQ=60)
                                 TAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTAGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATG                                                                                                                      <  SRR19973065.988434/150‑1 (MQ=60)
                                  AAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGC                                                                                                                     <  SRR19973065.775817/150‑1 (MQ=60)
                                  AAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGC                                                                                                                     <  SRR19973065.2546191/150‑1 (MQ=60)
                                  AAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGC                                                                                                                     <  SRR19973065.775825/150‑1 (MQ=60)
                                  AAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGC                                                                                                                     <  SRR19973065.1140469/150‑1 (MQ=60)
                                      GAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCG                                                                                                                 <  SRR19973065.1909847/150‑1 (MQ=60)
                                      GAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCG                                                                                                                 <  SRR19973065.2725665/150‑1 (MQ=60)
                                             TTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGT                                                                                                          >  SRR19973065.1830916/1‑150 (MQ=60)
                                               TTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTT                                                                                                        <  SRR19973065.1958964/150‑1 (MQ=60)
                                               TTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTT                                                                                                        <  SRR19973065.3061560/150‑1 (MQ=60)
                                                  CGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTG                                                                                                     >  SRR19973065.915724/1‑150 (MQ=60)
                                                  CGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTG                                                                                                     >  SRR19973065.915695/1‑150 (MQ=60)
                                                    CCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCC                                                                                                   <  SRR19973065.61716/150‑1 (MQ=60)
                                                    CCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCC                                                                                                   <  SRR19973065.58184/150‑1 (MQ=60)
                                                        AACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCA                                                                                               >  SRR19973065.1838407/1‑150 (MQ=60)
                                                            GTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGC                                                                                           >  SRR19973065.355970/1‑150 (MQ=60)
                                                             TGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCT                                                                                          <  SRR19973065.1537514/150‑1 (MQ=60)
                                                             TGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCAGTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCT                                                                                          <  SRR19973065.1910761/150‑1 (MQ=60)
                                                                 AGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAC                                                                                      >  SRR19973065.1482745/1‑150 (MQ=60)
                                                                   CAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTTTTTGTGCCGTCAGTGCTTTACAT                                                                                    <  SRR19973065.1481744/150‑1 (MQ=60)
                                                                   CAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACAT                                                                                    <  SRR19973065.3235213/150‑1 (MQ=60)
                                                                       TCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCG                                                                                <  SRR19973065.67385/150‑1 (MQ=60)
                                                                         GGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCT                                                                              >  SRR19973065.1956271/1‑150 (MQ=60)
                                                                            GTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAA                                                                           >  SRR19973065.2088688/1‑150 (MQ=60)
                                                                              AACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACA                                                                         >  SRR19973065.1714999/1‑150 (MQ=60)
                                                                                CCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGA                                                                       >  SRR19973065.1760127/1‑150 (MQ=60)
                                                                                 CACTGATGAGACGCTACGCCCGTAACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGTTGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGAT                                                                      <  SRR19973065.1919771/150‑1 (MQ=60)
                                                                                    TGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGC                                                                   >  SRR19973065.510520/1‑150 (MQ=60)
                                                                                       TGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCA                                                                <  SRR19973065.862166/150‑1 (MQ=60)
                                                                                       TGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCA                                                                <  SRR19973065.862421/150‑1 (MQ=60)
                                                                                       TGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCA                                                                >  SRR19973065.1719032/1‑150 (MQ=60)
                                                                                       TGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCA                                                                >  SRR19973065.1719033/1‑150 (MQ=60)
                                                                                         AGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAAC                                                              >  SRR19973065.1381600/1‑150 (MQ=60)
                                                                                              CTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCA                                                         <  SRR19973065.3332915/150‑1 (MQ=60)
                                                                                                ACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGG                                                       >  SRR19973065.3284658/1‑150 (MQ=60)
                                                                                                 CGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGA                                                      <  SRR19973065.1808530/150‑1 (MQ=60)
                                                                                                 CGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGA                                                      >  SRR19973065.944178/1‑150 (MQ=60)
                                                                                                   CCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGG                                                    <  SRR19973065.238096/150‑1 (MQ=60)
                                                                                                   CCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGG                                                    >  SRR19973065.1573989/1‑150 (MQ=60)
                                                                                                     CGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCC                                                  >  SRR19973065.2301970/1‑150 (MQ=60)
                                                                                                      GTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCA                                                 >  SRR19973065.2501514/1‑150 (MQ=60)
                                                                                                        CACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAAC                                               <  SRR19973065.1851304/150‑1 (MQ=60)
                                                                                                           AGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGCGGCCAACAAT                                            >  SRR19973065.120091/1‑150 (MQ=60)
                                                                                                           AGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAAT                                            <  SRR19973065.1439973/150‑1 (MQ=60)
                                                                                                           AGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAAT                                            >  SRR19973065.331531/1‑150 (MQ=60)
                                                                                                           AGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAAT                                            <  SRR19973065.1439955/150‑1 (MQ=60)
                                                                                                            GGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATG                                           <  SRR19973065.2710873/150‑1 (MQ=60)
                                                                                                            GGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATG                                           >  SRR19973065.2526619/1‑150 (MQ=60)
                                                                                                                GGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATG                                       <  SRR19973065.1829009/150‑1 (MQ=60)
                                                                                                                   CAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACA                                    >  SRR19973065.1596519/1‑150 (MQ=60)
                                                                                                                     AACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC                                  >  SRR19973065.1340968/1‑150 (MQ=60)
                                                                                                                     AACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC                                  >  SRR19973065.1340576/1‑150 (MQ=60)
                                                                                                                     AACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC                                  <  SRR19973065.2587994/150‑1 (MQ=60)
                                                                                                                     AACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC                                  >  SRR19973065.1340593/1‑150 (MQ=60)
                                                                                                                      ACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCA                                 <  SRR19973065.2287803/150‑1 (MQ=60)
                                                                                                                         ATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCT                              <  SRR19973065.906155/150‑1 (MQ=60)
                                                                                                                          TTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTT                             <  SRR19973065.211588/150‑1 (MQ=60)
                                                                                                                          TTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTT                             <  SRR19973065.211527/150‑1 (MQ=60)
                                                                                                                          TTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTT                             >  SRR19973065.1138990/1‑150 (MQ=60)
                                                                                                                            GAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCG                           >  SRR19973065.2565980/1‑150 (MQ=60)
                                                                                                                            GAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCG                           >  SRR19973065.796144/1‑150 (MQ=60)
                                                                                                                            GAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCG                           <  SRR19973065.249937/150‑1 (MQ=60)
                                                                                                                               AACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAA                        >  SRR19973065.2608035/1‑150 (MQ=60)
                                                                                                                                ACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAA                       >  SRR19973065.3209420/1‑150 (MQ=60)
                                                                                                                                 CCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAAC                      <  SRR19973065.631712/150‑1 (MQ=60)
                                                                                                                                 CCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAAC                      >  SRR19973065.547755/1‑150 (MQ=60)
                                                                                                                                 CCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAAC                      >  SRR19973065.2648508/1‑150 (MQ=60)
                                                                                                                                       TGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATT                <  SRR19973065.570619/150‑1 (MQ=60)
                                                                                                                                        GCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTA               <  SRR19973065.509099/150‑1 (MQ=60)
                                                                                                                                        GCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTA               <  SRR19973065.509033/150‑1 (MQ=60)
                                                                                                                                           ATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTTCCGTCAGTGCTTTACAAGCCGCTCAACAGATTGCCCAAAGCGCAGGAGGCCAACAATCAATGACACCAGCTTCGAAAACTGAATTAAGT            >  SRR19973065.3068200/1‑150 (MQ=60)
                                                                                                                                               GTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCAC        <  SRR19973065.749581/150‑1 (MQ=60)
                                                                                                                                                  CGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGT     >  SRR19973065.183527/1‑150 (MQ=60)
                                                                                                                                                     AGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTG  <  SRR19973065.1223581/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCACCGTCTG  >  CP009273/2348745‑2349043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑