Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,404,059 C→A 90.5% R141S (CGC→AGC)  dusB → tRNA‑dihydrouridine synthase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,404,0590CA90.5% 499.6 / 47.0 189R141S (CGC→AGC) dusBtRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base C (3/15);  new base A (101/70);  total (104/85)
Fisher's exact test for biased strand distribution p-value = 7.74e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 189 total aligned reads displayed.

GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203
                                                                                                                                                 |                                                                                                                                                
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                <  1:2503280/148‑1 (MQ=255)
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                >  2:1884970/1‑148 (MQ=255)
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                <  2:1817951/148‑1 (MQ=255)
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAATGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                >  2:1666311/1‑148 (MQ=255)
gATTATTGATATCAATACGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                <  2:3284005/148‑1 (MQ=255)
  ttattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAc                                                                                                                                              <  1:3065695/148‑1 (MQ=255)
       gATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCt                                                                                                                                         <  1:2998096/148‑1 (MQ=255)
          atCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTggg                                                                                                                                      >  2:829551/1‑148 (MQ=255)
            cAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCa                                                                                                                                    >  1:72222/1‑148 (MQ=255)
            cAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCa                                                                                                                                    >  1:72536/1‑148 (MQ=255)
               tatGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCg                                                                                                                                 >  1:782383/1‑148 (MQ=255)
               tatGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTACTGTTACCCTGAAGATTAGCACCGGCTGGGCACCg                                                                                                                                 >  1:2440651/1‑148 (MQ=255)
                 tGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGa                                                                                                                               <  2:39697/148‑1 (MQ=255)
                 tGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGa                                                                                                                               >  1:1279961/1‑148 (MQ=255)
                  gggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGaa                                                                                                                              >  2:2340717/1‑148 (MQ=255)
                  gggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGaa                                                                                                                              <  1:3298085/148‑1 (MQ=255)
                  gggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGaa                                                                                                                              >  1:2912024/1‑148 (MQ=255)
                    gTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAaca                                                                                                                            <  2:3081519/148‑1 (MQ=255)
                        cccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt                                                                                                                        <  2:1502161/148‑1 (MQ=255)
                            gCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACt                                                                                                                    <  2:1573123/148‑1 (MQ=255)
                                aaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGa                                                                                                                <  1:1562084/148‑1 (MQ=255)
                                aaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGa                                                                                                                <  1:1376401/148‑1 (MQ=255)
                                 aaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGaa                                                                                                               >  2:2015323/1‑148 (MQ=255)
                                  aaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAag                                                                                                              >  2:2288112/1‑148 (MQ=255)
                                  aaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAag                                                                                                              <  2:3229924/148‑1 (MQ=255)
                                  aaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAag                                                                                                              >  2:2080930/1‑148 (MQ=255)
                                  aaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGAACCGGCTGGGCACCGGAACACCGTAACTGCGAag                                                                                                              >  2:779098/1‑148 (MQ=255)
                                    aGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag                                                                                                            >  1:265150/1‑148 (MQ=255)
                                    aGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag                                                                                                            >  2:1307151/1‑148 (MQ=255)
                                    aGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag                                                                                                            >  2:1307006/1‑148 (MQ=255)
                                    aGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag                                                                                                            >  2:1306875/1‑148 (MQ=255)
                                      tGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAt                                                                                                          <  2:2635928/148‑1 (MQ=255)
                                       gAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAtt                                                                                                         >  1:2009430/1‑148 (MQ=255)
                                          tCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGcc                                                                                                      >  2:3220535/1‑148 (MQ=255)
                                          tCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGcc                                                                                                      >  1:2453818/1‑148 (MQ=255)
                                             cAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa                                                                                                   <  2:875690/148‑1 (MQ=255)
                                             cAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa                                                                                                   <  2:111960/148‑1 (MQ=255)
                                               aGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACt                                                                                                 >  1:101427/1‑148 (MQ=255)
                                                 cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTgg                                                                                               >  1:2262992/1‑148 (MQ=255)
                                                  tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGc                                                                                              <  1:1849741/148‑1 (MQ=255)
                                                       ggTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAg                                                                                         <  2:1385455/148‑1 (MQ=255)
                                                        gTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        >  2:2406702/1‑148 (MQ=255)
                                                           cAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtg                                                                                     >  2:655535/1‑148 (MQ=255)
                                                                ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCa                                                                                >  1:982096/1‑148 (MQ=255)
                                                                 tctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAt                                                                               >  1:2212780/1‑148 (MQ=255)
                                                                 tctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATAGCCCAACTGGCTGAAGACTGTGGCAt                                                                               >  2:24371/1‑148 (MQ=255)
                                                                  ctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAtt                                                                              <  1:691229/148‑1 (MQ=255)
                                                                   ttGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTc                                                                             >  2:2467722/1‑148 (MQ=255)
                                                                   ttGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTc                                                                             >  2:1033181/1‑148 (MQ=255)
                                                                        gTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGct                                                                        <  1:1500677/148‑1 (MQ=255)
                                                                         tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGctc                                                                       <  1:2727656/148‑1 (MQ=255)
                                                                         tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGctc                                                                       >  2:544979/1‑148 (MQ=255)
                                                                               gATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCa                                                                 <  1:2931324/148‑1 (MQ=255)
                                                                                 tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAtt                                                               >  1:314260/1‑148 (MQ=255)
                                                                                 tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAtt                                                               >  1:314077/1‑148 (MQ=255)
                                                                                  gTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTc                                                              <  2:919892/148‑1 (MQ=255)
                                                                                   tCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCa                                                             <  2:938519/148‑1 (MQ=255)
                                                                                   tCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCa                                                             <  2:531708/148‑1 (MQ=255)
                                                                                   tCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCa                                                             <  1:766626/148‑1 (MQ=255)
                                                                                       tAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGc                                                         >  2:2615680/1‑148 (MQ=255)
                                                                                           tCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTa                                                     >  1:1752566/1‑148 (MQ=255)
                                                                                            cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTac                                                    >  1:2104265/1‑148 (MQ=255)
                                                                                              aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTacac                                                  >  1:2850340/1‑148 (MQ=255)
                                                                                               tCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcg                                                 >  2:413300/1‑148 (MQ=255)
                                                                                               tCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcg                                                 >  2:1768363/1‑148 (MQ=255)
                                                                                                 cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcgcg                                               >  1:1109284/1‑148 (MQ=255)
                                                                                                 cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcgcg                                               <  2:72331/148‑1 (MQ=255)
                                                                                                   tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCCTTCATGGCCGTACACGCGcc                                             >  2:2660838/1‑148 (MQ=255)
                                                                                                   tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGcc                                             >  2:1007376/1‑148 (MQ=255)
                                                                                                     ccGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtg                                           <  2:625009/148‑1 (MQ=255)
                                                                                                      cGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgt                                          <  2:25310/148‑1 (MQ=255)
                                                                                                         ggTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttg                                       <  1:2339863/148‑1 (MQ=255)
                                                                                                           tcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgtt                                     <  2:373995/148‑1 (MQ=255)
                                                                                                            cgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTc                                    <  2:415130/148‑1 (MQ=255)
                                                                                                            cgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTc                                    >  1:2345749/1‑148 (MQ=255)
                                                                                                              tcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                  <  1:791495/148‑1 (MQ=255)
                                                                                                              tcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                  <  2:2912024/148‑1 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 >  1:1780875/1‑148 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 >  1:2002957/1‑148 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 <  1:2678519/148‑1 (MQ=255)
                                                                                                                aaTGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATg                                <  2:2136329/148‑1 (MQ=255)
                                                                                                                   gCAGTGGACGTTCCTGTTACCCTGAAGATTAGCTCCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgag                             <  2:3245935/148‑1 (MQ=255)
                                                                                                                   gCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgag                             <  2:3232943/148‑1 (MQ=255)
                                                                                                                   gCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgag                             <  2:3232959/148‑1 (MQ=255)
                                                                                                                    cAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgaga                            >  2:2113194/1‑148 (MQ=255)
                                                                                                                    cAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgaga                            <  1:2890817/148‑1 (MQ=255)
                                                                                                                      gTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAg                          <  1:2646311/148‑1 (MQ=255)
                                                                                                                      gTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAg                          <  1:1307151/148‑1 (MQ=255)
                                                                                                                      gTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAg                          <  1:1306893/148‑1 (MQ=255)
                                                                                                                             ttCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGACGCTGAGTa                   >  2:3023200/1‑148 (MQ=255)
                                                                                                                             ttCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTa                   >  1:953225/1‑148 (MQ=255)
                                                                                                                                cTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGa                <  1:1666311/148‑1 (MQ=255)
                                                                                                                                 tGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGAc               <  1:2080930/148‑1 (MQ=255)
                                                                                                                                  gTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACa              <  1:242387/148‑1 (MQ=255)
                                                                                                                                  gTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACa              <  1:413300/148‑1 (MQ=255)
                                                                                                                                  gTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACa              <  1:1768363/148‑1 (MQ=255)
                                                                                                                                   ttACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAg             <  1:2650685/148‑1 (MQ=255)
                                                                                                                                         tGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCg       >  2:134535/1‑148 (MQ=255)
                                                                                                                                             gATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCa   >  1:3083538/1‑148 (MQ=255)
                                                                                                                                             gATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCa   >  1:2300609/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  CP009273/3403912‑3404208
                                                                                                                                                   |                                                                                                                                                     
CAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGC                                                                                                                                                     >  SRR19973070.746918/1‑150 (MQ=60)
  GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   <  SRR19973070.2519536/150‑1 (MQ=60)
    TTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCG                                                                                                                                                 <  SRR19973070.3085342/150‑1 (MQ=60)
         GATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGG                                                                                                                                            <  SRR19973070.3017284/150‑1 (MQ=60)
            ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973070.73111/1‑150 (MQ=60)
            ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973070.2983829/1‑150 (MQ=60)
            ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973070.73002/1‑150 (MQ=60)
            ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973070.73318/1‑150 (MQ=60)
               AATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCG                                                                                                                                      >  SRR19973070.2456525/1‑150 (MQ=60)
               AATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCG                                                                                                                                      >  SRR19973070.787835/1‑150 (MQ=60)
               AATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTACTGTTACCCTGAAGATTAGCACCGGCTGGGCACCG                                                                                                                                      >  SRR19973070.2456644/1‑150 (MQ=60)
                 TATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGA                                                                                                                                    >  SRR19973070.2947582/1‑150 (MQ=60)
                 TATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGA                                                                                                                                    >  SRR19973070.1288905/1‑150 (MQ=60)
                  ATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAA                                                                                                                                   >  SRR19973070.2930847/1‑150 (MQ=60)
                    GGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACA                                                                                                                                 <  SRR19973070.3319216/150‑1 (MQ=60)
                                  AAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAG                                                                                                                   <  SRR19973070.1573076/150‑1 (MQ=60)
                                  AAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAG                                                                                                                   <  SRR19973070.1573078/150‑1 (MQ=60)
                                  AAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAG                                                                                                                   <  SRR19973070.1385745/150‑1 (MQ=60)
                                    AAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAG                                                                                                                 <  SRR19973070.2141960/150‑1 (MQ=60)
                                    AAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAG                                                                                                                 >  SRR19973070.266909/1‑150 (MQ=60)
                                    AAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAG                                                                                                                 >  SRR19973070.266426/1‑150 (MQ=60)
                                       GTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATT                                                                                                              >  SRR19973070.2023038/1‑150 (MQ=60)
                                       GTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATT                                                                                                              >  SRR19973070.2023031/1‑150 (MQ=60)
                                          AATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCC                                                                                                           >  SRR19973070.2469864/1‑150 (MQ=60)
                                               CAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACT                                                                                                      >  SRR19973070.102395/1‑150 (MQ=60)
                                                 AGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGACGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGG                                                                                                    <  SRR19973070.1507509/150‑1 (MQ=60)
                                                 AGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGG                                                                                                    >  SRR19973070.2278002/1‑150 (MQ=60)
                                                 AGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGG                                                                                                    >  SRR19973070.2278008/1‑150 (MQ=60)
                                                    TCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTG                                                                                                 <  SRR19973070.1862419/150‑1 (MQ=60)
                                                                CCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCA                                                                                     >  SRR19973070.989097/1‑150 (MQ=60)
                                                                CCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCA                                                                                     >  SRR19973070.989096/1‑150 (MQ=60)
                                                                 CCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAT                                                                                    >  SRR19973070.2227327/1‑150 (MQ=60)
                                                                    CTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCA                                                                                 <  SRR19973070.696168/150‑1 (MQ=60)
                                                                    CTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCA                                                                                 <  SRR19973070.697205/150‑1 (MQ=60)
                                                                        CAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCT                                                                             <  SRR19973070.276975/150‑1 (MQ=60)
                                                                          GTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCT                                                                           <  SRR19973070.1511188/150‑1 (MQ=60)
                                                                           TACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTG                                                                          <  SRR19973070.2745248/150‑1 (MQ=60)
                                                                           TACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTG                                                                          <  SRR19973070.2744900/150‑1 (MQ=60)
                                                                           TACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTG                                                                          <  SRR19973070.1364160/150‑1 (MQ=60)
                                                                                 GATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATT                                                                    >  SRR19973070.316343/1‑150 (MQ=60)
                                                                                 GATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATT                                                                    <  SRR19973070.2950235/150‑1 (MQ=60)
                                                                                 GATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATT                                                                    >  SRR19973070.316527/1‑150 (MQ=60)
                                                                                 GATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATT                                                                    >  SRR19973070.316367/1‑150 (MQ=60)
                                                                                  ATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTC                                                                   >  SRR19973070.2465663/1‑150 (MQ=60)
                                                                                     TCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATG                                                                <  SRR19973070.772009/150‑1 (MQ=60)
                                                                                       GTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGC                                                              >  SRR19973070.2645836/1‑150 (MQ=60)
                                                                                        TTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCC                                                             <  SRR19973070.185415/150‑1 (MQ=60)
                                                                                           AATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTA                                                          >  SRR19973070.1764671/1‑150 (MQ=60)
                                                                                           AATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTA                                                          >  SRR19973070.1764708/1‑150 (MQ=60)
                                                                                            ATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTAC                                                         >  SRR19973070.2118385/1‑150 (MQ=60)
                                                                                             TCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACA                                                        <  SRR19973070.2888493/150‑1 (MQ=60)
                                                                                              CGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAC                                                       >  SRR19973070.2868800/1‑150 (MQ=60)
                                                                                               GATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACG                                                      >  SRR19973070.3118836/1‑150 (MQ=60)
                                                                                               GATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACG                                                      >  SRR19973070.2693644/1‑150 (MQ=60)
                                                                                               GATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACG                                                      >  SRR19973070.231981/1‑150 (MQ=60)
                                                                                                ATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGC                                                     >  SRR19973070.1546775/1‑150 (MQ=60)
                                                                                                 TCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCG                                                    >  SRR19973070.1116877/1‑150 (MQ=60)
                                                                                                         GAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTG                                            >  SRR19973070.2485839/1‑150 (MQ=60)
                                                                                                          AGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACCCGCGCCTGTTTGT                                           >  SRR19973070.301936/1‑150 (MQ=60)
                                                                                                           GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          <  SRR19973070.2355390/150‑1 (MQ=60)
                                                                                                            GTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTC                                         >  SRR19973070.2361310/1‑150 (MQ=60)
                                                                                                            GTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTC                                         >  SRR19973070.2360802/1‑150 (MQ=60)
                                                                                                               GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973070.2921011/1‑150 (MQ=60)
                                                                                                               GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973070.2018237/1‑150 (MQ=60)
                                                                                                               GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973070.2016517/1‑150 (MQ=60)
                                                                                                               GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973070.3275564/1‑150 (MQ=60)
                                                                                                               GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973070.1793200/1‑150 (MQ=60)
                                                                                                                TCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATG                                     <  SRR19973070.796976/150‑1 (MQ=60)
                                                                                                                TCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATG                                     <  SRR19973070.796962/150‑1 (MQ=60)
                                                                                                                 CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973070.80270/1‑150 (MQ=60)
                                                                                                                 CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    <  SRR19973070.2695906/150‑1 (MQ=60)
                                                                                                                     GCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAA                                <  SRR19973070.1199234/150‑1 (MQ=60)
                                                                                                                     GCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAA                                <  SRR19973070.1910655/150‑1 (MQ=60)
                                                                                                                      CAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAG                               <  SRR19973070.2909527/150‑1 (MQ=60)
                                                                                                                        GTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCT                             <  SRR19973070.1315954/150‑1 (MQ=60)
                                                                                                                        GTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCT                             <  SRR19973070.1315972/150‑1 (MQ=60)
                                                                                                                        GTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCT                             <  SRR19973070.2663544/150‑1 (MQ=60)
                                                                                                                        GTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCT                             <  SRR19973070.1316086/150‑1 (MQ=60)
                                                                                                                        GTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCT                             <  SRR19973070.1316232/150‑1 (MQ=60)
                                                                                                                         TGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTG                            <  SRR19973070.2388815/150‑1 (MQ=60)
                                                                                                                             CGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTA                        >  SRR19973070.960032/1‑150 (MQ=60)
                                                                                                                             CGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTA                        >  SRR19973070.960092/1‑150 (MQ=60)
                                                                                                                               TTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACG                      >  SRR19973070.1787242/1‑150 (MQ=60)
                                                                                                                               TTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACG                      <  SRR19973070.2111793/150‑1 (MQ=60)
                                                                                                                                  CTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACA                   <  SRR19973070.1677758/150‑1 (MQ=60)
                                                                                                                                   TGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAG                  <  SRR19973070.2094932/150‑1 (MQ=60)
                                                                                                                                    GTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGT                 <  SRR19973070.1780603/150‑1 (MQ=60)
                                                                                                                                    GTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGT                 <  SRR19973070.244056/150‑1 (MQ=60)
                                                                                                                                    GTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGT                 <  SRR19973070.416314/150‑1 (MQ=60)
                                                                                                                                    GTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGT                 <  SRR19973070.415838/150‑1 (MQ=60)
                                                                                                                                     TTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTA                <  SRR19973070.518815/150‑1 (MQ=60)
                                                                                                                                     TTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTA                <  SRR19973070.2667932/150‑1 (MQ=60)
                                                                                                                                             AAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCA        >  SRR19973070.2315914/1‑150 (MQ=60)
                                                                                                                                             AAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCA        >  SRR19973070.3103365/1‑150 (MQ=60)
                                                                                                                                                ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     >  SRR19973070.724479/1‑150 (MQ=60)
                                                                                                                                                   AGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  SRR19973070.2819402/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  CP009273/3403912‑3404208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑