Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,404,059 C→A 100% R141S (CGC→AGC)  dusB → tRNA‑dihydrouridine synthase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,404,0590CA98.1% 684.1 / 3.2 213R141S (CGC→AGC) dusBtRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (100/109);  total (103/110)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203
                                                                                                                                                 |                                                                                                                                                
 attattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCa                                                                                                                                               <  1:51934/148‑1 (MQ=255)
 attattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCa                                                                                                                                               <  2:2377518/148‑1 (MQ=255)
  ttattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAc                                                                                                                                              >  1:338687/1‑148 (MQ=255)
  ttattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAc                                                                                                                                              >  1:338283/1‑148 (MQ=255)
   tattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAcc                                                                                                                                             >  1:1722712/1‑148 (MQ=255)
     ttGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCgg                                                                                                                                           >  1:1017751/1‑148 (MQ=255)
     ttGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGAAGTACCCGGATGTCGTTAAATCTATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCgg                                                                                                                                           >  1:2001525/1‑148 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGc                                                                                                                                          >  1:2855149/1‑148 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGc                                                                                                                                          >  2:318631/1‑148 (MQ=255)
       gATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCt                                                                                                                                         <  1:894981/148‑1 (MQ=255)
       gATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCt                                                                                                                                         <  1:348021/148‑1 (MQ=255)
          atCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTggg                                                                                                                                      >  2:2804899/1‑148 (MQ=255)
            cAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCa                                                                                                                                    <  2:21660/148‑1 (MQ=255)
            cAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCa                                                                                                                                    >  1:3154649/1‑148 (MQ=255)
             aaTATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCAc                                                                                                                                   <  1:2112873/148‑1 (MQ=255)
             aaTATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCAc                                                                                                                                   <  1:2157250/148‑1 (MQ=255)
               tatGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCg                                                                                                                                 <  1:247072/148‑1 (MQ=255)
                  gggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGaa                                                                                                                              >  1:2340655/1‑148 (MQ=255)
                  gggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGaa                                                                                                                              <  1:1774485/148‑1 (MQ=255)
                    gTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAaca                                                                                                                            <  2:1488254/148‑1 (MQ=255)
                    gTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAaca                                                                                                                            <  1:300377/148‑1 (MQ=255)
                         ccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTa                                                                                                                       <  2:2929618/148‑1 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  1:267193/148‑1 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  2:2362075/148‑1 (MQ=255)
                              tAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGc                                                                                                                  >  2:2634783/1‑148 (MQ=255)
                                 aaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGaa                                                                                                               <  1:3049217/148‑1 (MQ=255)
                                 aaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCT‑AAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAag                                                                                                              <  2:195553/148‑1 (MQ=255)
                                    aGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag                                                                                                            <  2:1176911/148‑1 (MQ=255)
                                      tGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAt                                                                                                          >  1:2958531/1‑148 (MQ=255)
                                      tGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAt                                                                                                          >  2:662327/1‑148 (MQ=255)
                                             cAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa                                                                                                   >  1:1967475/1‑148 (MQ=255)
                                              aaGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAAc                                                                                                  <  2:1128839/148‑1 (MQ=255)
                                               agttCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACt                                                                                                 >  1:1888859/4‑148 (MQ=255)
                                                gCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg                                                                                                >  1:2481107/1‑148 (MQ=255)
                                                gCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg                                                                                                >  1:2082523/1‑148 (MQ=255)
                                                     cAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGa                                                                                           <  1:2282660/148‑1 (MQ=255)
                                                        gctCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        <  1:1531649/146‑1 (MQ=255)
                                                        gTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        >  2:1372676/1‑148 (MQ=255)
                                                        gTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        >  2:3380034/1‑148 (MQ=255)
                                                            aGCCCTATTGCAGTACACTGATGTCGTTAAATGGATCCTTTCCGTGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAAAACCGTAACTGCGAAGAGTTTGACCAACTTGATGAATACtgt                                                                                    <  2:1717971/148‑1 (MQ=255)
                                                                ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGATCACCGTAACTGCGCAGAGATTGCCCAACTGGCTGAAGACTGTGGCa                                                                                <  2:765552/148‑1 (MQ=255)
                                                                ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCa                                                                                >  2:414833/1‑148 (MQ=255)
                                                                ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCa                                                                                >  1:262932/1‑148 (MQ=255)
                                                                 tctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAt                                                                               <  1:51240/148‑1 (MQ=255)
                                                                   ttGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTc                                                                             <  2:683115/148‑1 (MQ=255)
                                                                      cAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAgg                                                                          <  2:2156151/148‑1 (MQ=255)
                                                                        gTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGct                                                                        <  2:2146018/148‑1 (MQ=255)
                                                                           cccGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTg                                                                     <  1:441477/148‑1 (MQ=255)
                                                                              ggATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGAcc                                                                  <  1:1692571/148‑1 (MQ=255)
                                                                               gATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCa                                                                 <  2:3122571/148‑1 (MQ=255)
                                                                                aTGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAt                                                                >  2:1626192/1‑148 (MQ=255)
                                                                                 tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAtt                                                               >  1:2616340/1‑148 (MQ=255)
                                                                                 tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAtt                                                               >  2:2304689/1‑148 (MQ=255)
                                                                                   tCGTTACATCGATCCGGTCCGAGGTCGTCAATGCAGTGGACGTTCCGGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGACGAACGTACCTGCGAATAGATTGCACAACTGGCGGAAGAATGTGGCATTCAGGCTCTGACAATTCa                                                             <  2:1820333/148‑1 (MQ=255)
                                                                                   tCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCa                                                             >  2:2201859/1‑148 (MQ=255)
                                                                                   tCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCa                                                             <  1:2377558/148‑1 (MQ=255)
                                                                                    cGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCAt                                                            <  1:2071235/148‑1 (MQ=255)
                                                                                      ttAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATgg                                                          <  1:2047706/148‑1 (MQ=255)
                                                                                       tAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGc                                                         >  1:1456927/1‑148 (MQ=255)
                                                                                          aTCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGt                                                      >  2:2923131/1‑148 (MQ=255)
                                                                                            cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTac                                                    >  2:2594541/1‑148 (MQ=255)
                                                                                              aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTacac                                                  >  1:1211243/1‑148 (MQ=255)
                                                                                              aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTacac                                                  >  1:935779/1‑148 (MQ=255)
                                                                                                ccTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcgc                                                >  2:2221298/1‑148 (MQ=255)
                                                                                                  ttACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTACCTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACgcgc                                              <  1:2569826/148‑1 (MQ=255)
                                                                                                  ttACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACgcgc                                              >  2:3278715/1‑148 (MQ=255)
                                                                                                  ttACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACgcgc                                              <  1:3289298/148‑1 (MQ=255)
                                                                                                       gAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtt                                         <  1:341272/148‑1 (MQ=255)
                                                                                                         ggTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttg                                       <  1:57970/148‑1 (MQ=255)
                                                                                                           tcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgtt                                     <  1:77118/148‑1 (MQ=255)
                                                                                                           tcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgtt                                     >  1:297167/1‑148 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 >  1:464357/1‑148 (MQ=255)
                                                                                                                  tGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGga                              <  2:144017/148‑1 (MQ=255)
                                                                                                                    cAGTGGACGTTCCTGTTACCCTGAAGATTAGTACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgaga                            <  1:43411/148‑1 (MQ=255)
                                                                                                                    cAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgaga                            <  2:1640702/148‑1 (MQ=255)
                                                                                                                     aGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGaa                           <  1:1372676/148‑1 (MQ=255)
                                                                                                                      gTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAg                          >  2:2711083/1‑148 (MQ=255)
                                                                                                                      gTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAg                          <  2:2933168/148‑1 (MQ=255)
                                                                                                                       tGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGc                         <  1:630281/148‑1 (MQ=255)
                                                                                                                       tGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGc                         >  1:1711862/1‑148 (MQ=255)
                                                                                                                          aCGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGa                      >  1:1922122/1‑148 (MQ=255)
                                                                                                                            gTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGt                    >  1:1748044/1‑148 (MQ=255)
                                                                                                                             ttCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTa                   >  1:2358342/1‑148 (MQ=255)
                                                                                                                             ttCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTa                   >  1:595587/1‑148 (MQ=255)
                                                                                                                             ttCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTa                   <  1:1773845/148‑1 (MQ=255)
                                                                                                                              tCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTAc                  >  2:1090980/1‑148 (MQ=255)
                                                                                                                              tCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTAc                  >  1:1007405/1‑148 (MQ=255)
                                                                                                                                 tGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACGGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGAc               <  2:3310849/148‑1 (MQ=255)
                                                                                                                                  gTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACa              <  2:1967475/148‑1 (MQ=255)
                                                                                                                                    tACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGt            >  1:1386745/1‑148 (MQ=255)
                                                                                                                                    tACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGt            <  1:1255336/148‑1 (MQ=255)
                                                                                                                                    tACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGt            <  2:486545/148‑1 (MQ=255)
                                                                                                                                     aCCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTa           <  2:727973/148‑1 (MQ=255)
                                                                                                                                      cccTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTAt          <  2:2409182/148‑1 (MQ=255)
                                                                                                                                        cTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTc        <  1:2067295/148‑1 (MQ=255)
                                                                                                                                           aaGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCggg     >  2:1273097/1‑148 (MQ=255)
                                                                                                                                            aGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGc    <  2:1456927/148‑1 (MQ=255)
                                                                                                                                              aTTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAg  <  2:297167/148‑1 (MQ=255)
                                                                                                                                              aTTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAg  <  1:2634783/148‑1 (MQ=255)
                                                                                                                                              aTTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAg  >  1:2797609/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  CP009273/3403910‑3404208
                                                                                                                                                     |                                                                                                                                                     
CCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTA                                                                                                                                                       <  SRR19973065.3151729/150‑1 (MQ=60)
  CAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGC                                                                                                                                                     <  SRR19973065.1057327/150‑1 (MQ=60)
    GATTATTGATATCAATATGGGTTGCCCTGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   <  SRR19973065.2644788/150‑1 (MQ=60)
    GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   >  SRR19973065.340644/1‑150 (MQ=60)
    GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   >  SRR19973065.340648/1‑150 (MQ=60)
    GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   >  SRR19973065.341050/1‑150 (MQ=60)
     ATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACC                                                                                                                                                  >  SRR19973065.1889634/1‑150 (MQ=60)
     ATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACC                                                                                                                                                  <  SRR19973065.52504/150‑1 (MQ=60)
     ATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACC                                                                                                                                                  >  SRR19973065.1734150/1‑150 (MQ=60)
     ATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACC                                                                                                                                                  >  SRR19973065.1733941/1‑150 (MQ=60)
       TATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGG                                                                                                                                                >  SRR19973065.1024496/1‑150 (MQ=60)
       TATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGAAGTACCCGGATGTCGTTAAATCTATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGG                                                                                                                                                >  SRR19973065.2014476/1‑150 (MQ=60)
        ATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGC                                                                                                                                               >  SRR19973065.2872697/1‑150 (MQ=60)
         TTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCT                                                                                                                                              >  SRR19973065.1651722/1‑150 (MQ=60)
          TGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTG                                                                                                                                             <  SRR19973065.2305118/150‑1 (MQ=60)
           GATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGG                                                                                                                                            <  SRR19973065.900675/150‑1 (MQ=60)
           GATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGG                                                                                                                                            <  SRR19973065.350459/150‑1 (MQ=60)
            ATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGG                                                                                                                                           >  SRR19973065.687659/1‑150 (MQ=60)
              ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973065.3172368/1‑150 (MQ=60)
              ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973065.3173783/1‑150 (MQ=60)
               TCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCAC                                                                                                                                        >  SRR19973065.2616844/1‑150 (MQ=60)
               TCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCAC                                                                                                                                        >  SRR19973065.2616902/1‑150 (MQ=60)
                CAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACC                                                                                                                                       >  SRR19973065.1410896/1‑150 (MQ=60)
                CAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACC                                                                                                                                       >  SRR19973065.1649514/1‑150 (MQ=60)
                 AATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCG                                                                                                                                      >  SRR19973065.2289616/1‑150 (MQ=60)
                 AATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCG                                                                                                                                      <  SRR19973065.2126473/150‑1 (MQ=60)
                 AATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCG                                                                                                                                      <  SRR19973065.2171119/150‑1 (MQ=60)
                   TATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGA                                                                                                                                    <  SRR19973065.248818/150‑1 (MQ=60)
                    ATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAA                                                                                                                                   >  SRR19973065.2355269/1‑150 (MQ=60)
                    ATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAA                                                                                                                                   >  SRR19973065.2355289/1‑150 (MQ=60)
                     TGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAAC                                                                                                                                  >  SRR19973065.149569/1‑150 (MQ=60)
                     TGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAAC                                                                                                                                  <  SRR19973065.583447/150‑1 (MQ=60)
                     TGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAACCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAAC                                                                                                                                  <  SRR19973065.2084057/150‑1 (MQ=60)
                      GGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACA                                                                                                                                 <  SRR19973065.1786093/150‑1 (MQ=60)
                      GGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACA                                                                                                                                 <  SRR19973065.1785519/150‑1 (MQ=60)
                        GTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACC                                                                                                                               <  SRR19973065.302328/150‑1 (MQ=60)
                        GTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACC                                                                                                                               <  SRR19973065.302307/150‑1 (MQ=60)
                              CGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACT                                                                                                                         >  SRR19973065.2977812/1‑150 (MQ=60)
                              CGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACT                                                                                                                         <  SRR19973065.269013/150‑1 (MQ=60)
                                    AAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAG                                                                                                                   <  SRR19973065.1756675/150‑1 (MQ=60)
                                     AAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGA                                                                                                                  <  SRR19973065.3067870/150‑1 (MQ=60)
                                        AGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAT                                                                                                               >  SRR19973065.2976596/1‑150 (MQ=60)
                                         GTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATT                                                                                                              <  SRR19973065.1332997/150‑1 (MQ=60)
                                             ATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCC                                                                                                          >  SRR19973065.465752/1‑150 (MQ=60)
                                               CGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAA                                                                                                        >  SRR19973065.1980312/1‑150 (MQ=60)
                                                 CAAGTTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACT                                                                                                      >  SRR19973065.1901280/1‑150 (MQ=60)
                                                  AAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTG                                                                                                     >  SRR19973065.2095869/1‑150 (MQ=60)
                                                  AAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTG                                                                                                     >  SRR19973065.2496726/1‑150 (MQ=60)
                                                  AAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTG                                                                                                     >  SRR19973065.1918518/1‑150 (MQ=60)
                                                     CTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCT                                                                                                  >  SRR19973065.922154/1‑150 (MQ=60)
                                                      TCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTG                                                                                                 <  SRR19973065.2998763/150‑1 (MQ=60)
                                                         CAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAG                                                                                              <  SRR19973065.2297054/150‑1 (MQ=60)
                                                          AGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGA                                                                                             >  SRR19973065.2280470/1‑150 (MQ=60)
                                                            GCTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACT                                                                                           <  SRR19973065.1541740/150‑1 (MQ=60)
                                                                  CCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCA                                                                                     >  SRR19973065.264735/1‑150 (MQ=60)
                                                                     TCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTC                                                                                  <  SRR19973065.52099/150‑1 (MQ=60)
                                                                     TCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTC                                                                                  <  SRR19973065.51808/150‑1 (MQ=60)
                                                                     TCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTC                                                                                  <  SRR19973065.51801/150‑1 (MQ=60)
                                                                      CTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCA                                                                                 >  SRR19973065.2093914/1‑150 (MQ=60)
                                                                       TTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAG                                                                                <  SRR19973065.890020/150‑1 (MQ=60)
                                                                               CCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGAC                                                                        <  SRR19973065.444534/150‑1 (MQ=60)
                                                                                  GGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAT                                                                     <  SRR19973065.1703874/150‑1 (MQ=60)
                                                                                   GATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATT                                                                    >  SRR19973065.2632550/1‑150 (MQ=60)
                                                                                       TCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATG                                                                <  SRR19973065.2392392/150‑1 (MQ=60)
                                                                                       TCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATG                                                                <  SRR19973065.2392417/150‑1 (MQ=60)
                                                                                        CGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGG                                                               >  SRR19973065.3258885/1‑150 (MQ=60)
                                                                                        CGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGG                                                               <  SRR19973065.2084447/150‑1 (MQ=60)
                                                                                         GTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGC                                                              >  SRR19973065.3353495/1‑150 (MQ=60)
                                                                                         GTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGC                                                              >  SRR19973065.1466305/1‑150 (MQ=60)
                                                                                          TTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCC                                                             <  SRR19973065.2060787/150‑1 (MQ=60)
                                                                                          TTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCC                                                             <  SRR19973065.2060797/150‑1 (MQ=60)
                                                                                          TTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCC                                                             <  SRR19973065.2060816/150‑1 (MQ=60)
                                                                                            AAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGT                                                           <  SRR19973065.3028507/150‑1 (MQ=60)
                                                                                                CGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAC                                                       >  SRR19973065.941962/1‑150 (MQ=60)
                                                                                                CGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAC                                                       >  SRR19973065.1219065/1‑150 (MQ=60)
                                                                                                CGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAC                                                       >  SRR19973065.1219584/1‑150 (MQ=60)
                                                                                                     CTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCC                                                  >  SRR19973065.2167933/1‑150 (MQ=60)
                                                                                                      TTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTACCTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCT                                                 <  SRR19973065.2585869/150‑1 (MQ=60)
                                                                                                      TTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCT                                                 <  SRR19973065.3309312/150‑1 (MQ=60)
                                                                                                        ACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGT                                               >  SRR19973065.2456356/1‑150 (MQ=60)
                                                                                                          CGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTT                                             <  SRR19973065.564224/150‑1 (MQ=60)
                                                                                                           GAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTG                                            <  SRR19973065.343707/150‑1 (MQ=60)
                                                                                                           GAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTG                                            <  SRR19973065.343584/150‑1 (MQ=60)
                                                                                                           GAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTG                                            <  SRR19973065.343656/150‑1 (MQ=60)
                                                                                                             GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          <  SRR19973065.2910905/150‑1 (MQ=60)
                                                                                                             GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          <  SRR19973065.258546/150‑1 (MQ=60)
                                                                                                             GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          >  SRR19973065.299085/1‑150 (MQ=60)
                                                                                                             GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          <  SRR19973065.58589/150‑1 (MQ=60)
                                                                                                              GTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTC                                         >  SRR19973065.2115275/1‑150 (MQ=60)
                                                                                                               TCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCA                                        <  SRR19973065.77902/150‑1 (MQ=60)
                                                                                                                 GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973065.467561/1‑150 (MQ=60)
                                                                                                                 GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      <  SRR19973065.2672137/150‑1 (MQ=60)
                                                                                                                   CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973065.385247/1‑150 (MQ=60)
                                                                                                                   CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973065.385037/1‑150 (MQ=60)
                                                                                                                      TGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTAAATGGAGA                                 >  SRR19973065.441490/1‑150 (MQ=60)
                                                                                                                       GCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAA                                <  SRR19973065.1385107/150‑1 (MQ=60)
                                                                                                                        CAGTGGACGTTCCTGTTACCCTGAAGATTAGTACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAG                               <  SRR19973065.43928/150‑1 (MQ=60)
                                                                                                                         AGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGC                              <  SRR19973065.1953338/150‑1 (MQ=60)
                                                                                                                         AGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGC                              <  SRR19973065.1381735/150‑1 (MQ=60)
                                                                                                                         AGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGC                              >  SRR19973065.1723253/1‑150 (MQ=60)
                                                                                                                         AGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCATAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGC                              <  SRR19973065.1711940/150‑1 (MQ=60)
                                                                                                                           TGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTG                            <  SRR19973065.634380/150‑1 (MQ=60)
                                                                                                                           TGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTG                            <  SRR19973065.634379/150‑1 (MQ=60)
                                                                                                                           TGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTG                            <  SRR19973065.619182/150‑1 (MQ=60)
                                                                                                                            GGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGA                           >  SRR19973065.1934727/1‑150 (MQ=60)
                                                                                                                             GACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAG                          >  SRR19973065.1997872/1‑150 (MQ=60)
                                                                                                                              ACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGT                         >  SRR19973065.1759561/1‑150 (MQ=60)
                                                                                                                               CGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTA                        >  SRR19973065.599281/1‑150 (MQ=60)
                                                                                                                               CGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTA                        >  SRR19973065.2373001/1‑150 (MQ=60)
                                                                                                                                GTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTAC                       >  SRR19973065.1014090/1‑150 (MQ=60)
                                                                                                                                GTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTAC                       >  SRR19973065.1014124/1‑150 (MQ=60)
                                                                                                                                 TTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACG                      <  SRR19973065.1785453/150‑1 (MQ=60)
                                                                                                                                  TCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGA                     >  SRR19973065.1097536/1‑150 (MQ=60)
                                                                                                                                      GTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGT                 >  SRR19973065.1395857/1‑150 (MQ=60)
                                                                                                                                       TTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTA                <  SRR19973065.2835921/150‑1 (MQ=60)
                                                                                                                                        TACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTAT               <  SRR19973065.1263600/150‑1 (MQ=60)
                                                                                                                                          CCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTC             >  SRR19973065.1774938/1‑150 (MQ=60)
                                                                                                                                          CCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTC             <  SRR19973065.275628/150‑1 (MQ=60)
                                                                                                                                            CTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGG           <  SRR19973065.2080464/150‑1 (MQ=60)
                                                                                                                                            CTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGG           <  SRR19973065.2496714/150‑1 (MQ=60)
                                                                                                                                             TGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGG          >  SRR19973065.19551/1‑150 (MQ=60)
                                                                                                                                                AGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG       >  SRR19973065.2814942/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     <  SRR19973065.2651060/150‑1 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     >  SRR19973065.1161243/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     <  SRR19973065.2650967/150‑1 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     >  SRR19973065.2921272/1‑150 (MQ=60)
                                                                                                                                                    TAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAA   >  SRR19973065.3179612/1‑150 (MQ=60)
                                                                                                                                                     AGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  <  SRR19973065.1040397/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  CP009273/3403910‑3404208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑