Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,404,059 C→A 100% R141S (CGC→AGC)  dusB → tRNA‑dihydrouridine synthase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,404,0590CA99.5% 757.7 / ‑4.3 231R141S (CGC→AGC) dusBtRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (123/106);  total (125/106)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203
                                                                                                                                                 |                                                                                                                                                
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAGAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                <  1:747302/148‑1 (MQ=255)
gATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGc                                                                                                                                                <  1:747559/148‑1 (MQ=255)
 attattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCa                                                                                                                                               <  1:2279011/148‑1 (MQ=255)
  ttattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAc                                                                                                                                              >  2:681321/1‑148 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTTCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGc                                                                                                                                          >  2:53261/1‑148 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGACGATTAGCACCGGc                                                                                                                                          >  2:3489853/1‑148 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGc                                                                                                                                          >  1:1177325/1‑148 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGc                                                                                                                                          <  1:3276323/148‑1 (MQ=255)
      tGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGc                                                                                                                                          >  2:53806/1‑148 (MQ=255)
        atatCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCGTGAAGTTTAGCACCGGCTg                                                                                                                                        >  1:2198031/1‑148 (MQ=255)
          atCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTggg                                                                                                                                      >  2:585666/1‑148 (MQ=255)
           tCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGc                                                                                                                                     >  1:1419466/1‑148 (MQ=255)
            cAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCa                                                                                                                                    >  2:2723616/1‑148 (MQ=255)
            cAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCa                                                                                                                                    >  2:1249618/1‑148 (MQ=255)
               tatGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCATTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCg                                                                                                                                 >  1:3020546/1‑148 (MQ=255)
                atGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCgg                                                                                                                                >  2:3278415/1‑148 (MQ=255)
                   ggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAac                                                                                                                             >  1:430116/1‑148 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      >  2:3073816/1‑148 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  1:651682/148‑1 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  1:2626468/148‑1 (MQ=255)
                          cGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTaa                                                                                                                      <  2:1177548/148‑1 (MQ=255)
                            gCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACt                                                                                                                    >  1:2954475/1‑148 (MQ=255)
                               aaaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCg                                                                                                                 >  2:1437238/1‑148 (MQ=255)
                               aaaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCg                                                                                                                 >  2:1382846/1‑148 (MQ=255)
                               aaaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCg                                                                                                                 >  2:3396332/1‑148 (MQ=255)
                                aaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGa                                                                                                                <  2:2880588/148‑1 (MQ=255)
                                 aaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGaa                                                                                                               <  2:678914/148‑1 (MQ=255)
                                    aGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag                                                                                                            <  1:3485822/148‑1 (MQ=255)
                                    aGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag                                                                                                            >  2:3205531/1‑148 (MQ=255)
                                     gTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAgaga                                                                                                           <  2:2286358/148‑1 (MQ=255)
                                     gTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAgaga                                                                                                           <  1:580587/148‑1 (MQ=255)
                                     gTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAgaga                                                                                                           <  1:3612958/148‑1 (MQ=255)
                                        aaTCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTg                                                                                                        >  1:3804362/1‑148 (MQ=255)
                                        aaTCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTg                                                                                                        <  1:3514812/148‑1 (MQ=255)
                                          tCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGcc                                                                                                      >  2:3307388/1‑148 (MQ=255)
                                          tCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGcc                                                                                                      <  1:1083627/148‑1 (MQ=255)
                                           cGCAAGCTCGAAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTACTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGccc                                                                                                     >  2:926022/1‑148 (MQ=255)
                                             cAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa                                                                                                   <  2:3553035/148‑1 (MQ=255)
                                                gCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg                                                                                                >  1:1555361/1‑148 (MQ=255)
                                                gCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACGGCGAAGAGAGTGCCCAACTg                                                                                                >  1:2516708/1‑148 (MQ=255)
                                                 cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTgg                                                                                               >  2:2876629/1‑148 (MQ=255)
                                                     cAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGa                                                                                           <  2:103406/148‑1 (MQ=255)
                                                        gTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        <  2:1194784/148‑1 (MQ=255)
                                                        gTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        >  2:350574/1‑148 (MQ=255)
                                                        gTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGa                                                                                        <  1:2013058/148‑1 (MQ=255)
                                                         ttCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGAc                                                                                       >  2:1341425/1‑148 (MQ=255)
                                                           cAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtg                                                                                     <  1:2475340/148‑1 (MQ=255)
                                                             gCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgtg                                                                                   >  1:3390473/1‑148 (MQ=255)
                                                              cccTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg                                                                                  <  1:529157/148‑1 (MQ=255)
                                                                 tctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAt                                                                               >  1:1240675/1‑148 (MQ=255)
                                                                  ctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAtt                                                                              <  1:1963975/148‑1 (MQ=255)
                                                                   ttGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTc                                                                             <  2:255079/148‑1 (MQ=255)
                                                                      cAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAgg                                                                          >  1:173100/1‑148 (MQ=255)
                                                                          aCCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGctct                                                                      <  2:1718038/148‑1 (MQ=255)
                                                                            ccGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGa                                                                    >  2:3783697/1‑148 (MQ=255)
                                                                                aTGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAt                                                                >  2:2050935/1‑148 (MQ=255)
                                                                                 tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAtt                                                               >  2:3377224/1‑148 (MQ=255)
                                                                                  gTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTc                                                              <  2:2008939/148‑1 (MQ=255)
                                                                                  gTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTc                                                              <  1:2500433/148‑1 (MQ=255)
                                                                                     gTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATg                                                           <  2:1795568/148‑1 (MQ=255)
                                                                                      ttAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATgg                                                          >  1:1811186/1‑148 (MQ=255)
                                                                                      ttAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATgg                                                          >  2:3077929/1‑148 (MQ=255)
                                                                                       tAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGc                                                         >  2:888470/1‑148 (MQ=255)
                                                                                         aaTCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGAACCGGATGGGCACCGGAACACCGTAACTGCGAAGAGATAGCCCAACTGGCTGAAGACTGTGGCATTCATGCTCTGACCATTCATGGCCg                                                       >  1:3411176/1‑148 (MQ=255)
                                                                                           tCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTa                                                     >  1:2559237/1‑148 (MQ=255)
                                                                                            cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTac                                                    >  1:1532547/1‑148 (MQ=255)
                                                                                               tCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACAcg                                                 >  2:815334/1‑148 (MQ=255)
                                                                                                    aCCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCt                                            <  1:3566643/148‑1 (MQ=255)
                                                                                                     ccGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCACCTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtg                                           <  1:1723052/148‑1 (MQ=255)
                                                                                                     ccGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtg                                           <  1:921168/148‑1 (MQ=255)
                                                                                                     ccGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtg                                           <  2:26109/148‑1 (MQ=255)
                                                                                                         ggTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttg                                       <  1:3191697/148‑1 (MQ=255)
                                                                                                          gtcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGAGTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgt                                      >  2:2312602/1‑148 (MQ=255)
                                                                                                          gtcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgt                                      >  2:241693/1‑148 (MQ=255)
                                                                                                           tcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgtt                                     >  1:3243377/1‑148 (MQ=255)
                                                                                                           tcgtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCtgtttgtt                                     <  1:681321/148‑1 (MQ=255)
                                                                                                             gtcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCa                                   <  1:312949/148‑1 (MQ=255)
                                                                                                              tcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                  >  2:3066580/1‑148 (MQ=255)
                                                                                                              tcAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCaa                                  >  2:1854427/1‑148 (MQ=255)
                                                                                                               cAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAt                                 >  1:792707/1‑148 (MQ=255)
                                                                                                                 aTGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATgg                               >  1:1663215/1‑148 (MQ=255)
                                                                                                                 aTGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATgg                               >  1:3519242/1‑148 (MQ=255)
                                                                                                                 aTGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATgg                               >  1:269395/1‑148 (MQ=255)
                                                                                                                  tGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGga                              <  2:3616529/148‑1 (MQ=255)
                                                                                                                    cAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGgaga                            <  1:3156156/148‑1 (MQ=255)
                                                                                                                     aGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGaa                           >  1:2960116/1‑148 (MQ=255)
                                                                                                                       tGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGc                         >  1:1452561/1‑148 (MQ=255)
                                                                                                                       tGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGc                         >  1:311662/1‑148 (MQ=255)
                                                                                                                        ggACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCt                        <  1:1217252/148‑1 (MQ=255)
                                                                                                                         gACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTg                       <  1:2473346/148‑1 (MQ=255)
                                                                                                                             ttCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTa                   >  2:294311/1‑148 (MQ=255)
                                                                                                                                 tGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGAc               <  2:457782/148‑1 (MQ=255)
                                                                                                                                  gTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACa              <  2:3478865/148‑1 (MQ=255)
                                                                                                                                    tACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGt            <  1:1382846/148‑1 (MQ=255)
                                                                                                                                      cccTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTAt          <  2:2516259/148‑1 (MQ=255)
                                                                                                                                         tGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCg       <  2:413960/148‑1 (MQ=255)
                                                                                                                                         tGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCg       <  2:2336711/148‑1 (MQ=255)
                                                                                                                                          gAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCgg      >  2:75520/1‑148 (MQ=255)
                                                                                                                                             gATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCa   >  2:1303775/1‑148 (MQ=255)
                                                                                                                                             gATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCa   >  1:2872806/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG  >  CP009273/3403914‑3404203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  CP009273/3403910‑3404208
                                                                                                                                                     |                                                                                                                                                     
CCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTA                                                                                                                                                       <  SRR19973069.3803493/150‑1 (MQ=60)
 CCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAG                                                                                                                                                      <  SRR19973069.1052503/150‑1 (MQ=60)
 CCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAG                                                                                                                                                      <  SRR19973069.1052089/150‑1 (MQ=60)
  CAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGC                                                                                                                                                     <  SRR19973069.107992/150‑1 (MQ=60)
  CAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGC                                                                                                                                                     <  SRR19973069.2390606/150‑1 (MQ=60)
    GATTATTGATATCAATATGGGTTGCCCGGCTAAATAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCTGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   <  SRR19973069.752728/150‑1 (MQ=60)
    GATTATTGATATCAATATGGGTTGCCCGGCTAAAAGAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   <  SRR19973069.752597/150‑1 (MQ=60)
    GATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCAC                                                                                                                                                   <  SRR19973069.752855/150‑1 (MQ=60)
     ATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACC                                                                                                                                                  <  SRR19973069.2294485/150‑1 (MQ=60)
       TATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGG                                                                                                                                                <  SRR19973069.318695/150‑1 (MQ=60)
        ATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGC                                                                                                                                               >  SRR19973069.1185470/1‑150 (MQ=60)
        ATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGC                                                                                                                                               >  SRR19973069.1185694/1‑150 (MQ=60)
          TGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCGTGAAGTTTAGCACCGGCTG                                                                                                                                             >  SRR19973069.2212960/1‑150 (MQ=60)
          TGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTG                                                                                                                                             <  SRR19973069.3297775/150‑1 (MQ=60)
            ATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGG                                                                                                                                           >  SRR19973069.2492378/1‑150 (MQ=60)
             TATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGC                                                                                                                                          >  SRR19973069.1429370/1‑150 (MQ=60)
             TATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGC                                                                                                                                          >  SRR19973069.1429380/1‑150 (MQ=60)
              ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973069.3010637/1‑150 (MQ=60)
              ATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCA                                                                                                                                         >  SRR19973069.1091571/1‑150 (MQ=60)
                 AATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCATTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCG                                                                                                                                      >  SRR19973069.3040578/1‑150 (MQ=60)
                     TGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAAC                                                                                                                                  >  SRR19973069.433281/1‑150 (MQ=60)
                        GTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACC                                                                                                                               <  SRR19973069.1832198/150‑1 (MQ=60)
                              CGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACT                                                                                                                         >  SRR19973069.2974087/1‑150 (MQ=60)
                              CGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACT                                                                                                                         <  SRR19973069.2644045/150‑1 (MQ=60)
                              CGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACT                                                                                                                         <  SRR19973069.2644044/150‑1 (MQ=60)
                              CGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACT                                                                                                                         <  SRR19973069.655632/150‑1 (MQ=60)
                              CGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACT                                                                                                                         <  SRR19973069.656024/150‑1 (MQ=60)
                                       AAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGA                                                                                                                <  SRR19973069.3224869/150‑1 (MQ=60)
                                       AAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGA                                                                                                                >  SRR19973069.1249843/1‑150 (MQ=60)
                                        AGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAT                                                                                                               <  SRR19973069.3520287/150‑1 (MQ=60)
                                        AGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGAT                                                                                                               <  SRR19973069.3508318/150‑1 (MQ=60)
                                         GTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATT                                                                                                              <  SRR19973069.584572/150‑1 (MQ=60)
                                         GTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATT                                                                                                              <  SRR19973069.3636550/150‑1 (MQ=60)
                                          TGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTG                                                                                                             <  SRR19973069.425652/150‑1 (MQ=60)
                                          TGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTG                                                                                                             <  SRR19973069.425902/150‑1 (MQ=60)
                                          TGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTG                                                                                                             >  SRR19973069.3828996/1‑150 (MQ=60)
                                            AATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCC                                                                                                           <  SRR19973069.3537482/150‑1 (MQ=60)
                                              TCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCA                                                                                                         <  SRR19973069.1869774/150‑1 (MQ=60)
                                              TCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCA                                                                                                         <  SRR19973069.1091195/150‑1 (MQ=60)
                                                  AAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTG                                                                                                     >  SRR19973069.2526927/1‑150 (MQ=60)
                                                  AAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTG                                                                                                     >  SRR19973069.1565992/1‑150 (MQ=60)
                                                  AAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACGGCGAAGAGAGTGCCCAACTG                                                                                                     >  SRR19973069.2533456/1‑150 (MQ=60)
                                                     CTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCT                                                                                                  >  SRR19973069.2830520/1‑150 (MQ=60)
                                                            GTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACT                                                                                           <  SRR19973069.2027057/150‑1 (MQ=60)
                                                              TCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGTTGTCGTCAATGCAGTGGACGTTCCTGTTACACTGAAGATTAGCTCCGGCTGGGCACCGGAACAACGTAACTGCGAAGAGATTGCCCACCTGGCTGAAGACTGT                                                                                         <  SRR19973069.2522807/150‑1 (MQ=60)
                                                               CAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTG                                                                                        >  SRR19973069.3412619/1‑150 (MQ=60)
                                                               CAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTG                                                                                        <  SRR19973069.1491599/150‑1 (MQ=60)
                                                               CAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTG                                                                                        <  SRR19973069.2491933/150‑1 (MQ=60)
                                                                AGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGG                                                                                       >  SRR19973069.517253/1‑150 (MQ=60)
                                                                  CCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCA                                                                                     <  SRR19973069.532889/150‑1 (MQ=60)
                                                                   CCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCAT                                                                                    >  SRR19973069.1249108/1‑150 (MQ=60)
                                                                    CTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATT                                                                                   >  SRR19973069.1552982/1‑150 (MQ=60)
                                                                      CTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCA                                                                                 <  SRR19973069.1977598/150‑1 (MQ=60)
                                                                        TGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCACCTGGCTGAAGACTGTGGCATTCAGG                                                                               <  SRR19973069.2653678/150‑1 (MQ=60)
                                                                        TGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGG                                                                               >  SRR19973069.174896/1‑150 (MQ=60)
                                                                        TGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGG                                                                               >  SRR19973069.174565/1‑150 (MQ=60)
                                                                        TGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGG                                                                               >  SRR19973069.174566/1‑150 (MQ=60)
                                                                            GTACCCGGATGTCGTTAAATCGATCCTTTCCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCT                                                                           <  SRR19973069.3543681/150‑1 (MQ=60)
                                                                            GTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCT                                                                           <  SRR19973069.3407685/150‑1 (MQ=60)
                                                                                  GGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCAT                                                                     >  SRR19973069.2415939/1‑150 (MQ=60)
                                                                                   GATGTAGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATT                                                                    <  SRR19973069.3419833/150‑1 (MQ=60)
                                                                                      GTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCAT                                                                 <  SRR19973069.2517132/150‑1 (MQ=60)
                                                                                        CGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGG                                                               <  SRR19973069.1703685/150‑1 (MQ=60)
                                                                                        CGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGG                                                               >  SRR19973069.1823918/1‑150 (MQ=60)
                                                                                        CGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGG                                                               <  SRR19973069.3607894/150‑1 (MQ=60)
                                                                                         GTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGC                                                              >  SRR19973069.2975324/1‑150 (MQ=60)
                                                                                           TAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCG                                                            >  SRR19973069.3433458/1‑150 (MQ=60)
                                                                                           TAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGAACCGGATGGGCACCGGAACACCGTAACTGCGAAGAGATAGCCCAACTGGCTGAAGACTGTGGCATTCATGCTCTGACCATTCATGGCCG                                                            >  SRR19973069.3433400/1‑150 (MQ=60)
                                                                                            AAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTTAAGATTAGCACCCGCTGGGCACCGCAACACCGTAAATGCGAAGAGATTTCCCAACTGGATGAAGACTGTGGCATTCAGGCTCTtagcattaaagtccga                                                           >  SRR19973069.15110/1‑134 (MQ=60)
                                                                                            AAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGT                                                           >  SRR19973069.315643/1‑150 (MQ=60)
                                                                                             AATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTA                                                          >  SRR19973069.2576139/1‑150 (MQ=60)
                                                                                             AATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTA                                                          >  SRR19973069.2576131/1‑150 (MQ=60)
                                                                                             AATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTA                                                          >  SRR19973069.2576104/1‑150 (MQ=60)
                                                                                              ATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTAC                                                         >  SRR19973069.683400/1‑150 (MQ=60)
                                                                                              ATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTAC                                                         >  SRR19973069.1543076/1‑150 (MQ=60)
                                                                                                       TACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTG                                                <  SRR19973069.679352/150‑1 (MQ=60)
                                                                                                       TACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTG                                                <  SRR19973069.896485/150‑1 (MQ=60)
                                                                                                        ACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGT                                               <  SRR19973069.3589866/150‑1 (MQ=60)
                                                                                                         CCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCACCTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTT                                              <  SRR19973069.1735130/150‑1 (MQ=60)
                                                                                                         CCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTT                                              <  SRR19973069.927441/150‑1 (MQ=60)
                                                                                                         CCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATAGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTT                                              <  SRR19973069.3639622/150‑1 (MQ=60)
                                                                                                          CGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTT                                             >  SRR19973069.2379765/1‑150 (MQ=60)
                                                                                                          CGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTT                                             <  SRR19973069.77543/150‑1 (MQ=60)
                                                                                                             GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          <  SRR19973069.3212420/150‑1 (MQ=60)
                                                                                                             GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          <  SRR19973069.165569/150‑1 (MQ=60)
                                                                                                             GGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTT                                          >  SRR19973069.3264603/1‑150 (MQ=60)
                                                                                                               TCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCA                                        <  SRR19973069.2916550/150‑1 (MQ=60)
                                                                                                               TCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCA                                        <  SRR19973069.686056/150‑1 (MQ=60)
                                                                                                                 GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973069.2010234/1‑150 (MQ=60)
                                                                                                                 GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      <  SRR19973069.315031/150‑1 (MQ=60)
                                                                                                                 GTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAAT                                      >  SRR19973069.798335/1‑150 (MQ=60)
                                                                                                                   CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973069.1676608/1‑150 (MQ=60)
                                                                                                                   CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973069.271306/1‑150 (MQ=60)
                                                                                                                   CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973069.3541962/1‑150 (MQ=60)
                                                                                                                   CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973069.1674743/1‑150 (MQ=60)
                                                                                                                   CAATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGG                                    >  SRR19973069.3541961/1‑150 (MQ=60)
                                                                                                                    AATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGA                                   >  SRR19973069.3279266/1‑150 (MQ=60)
                                                                                                                     ATGCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAG                                  <  SRR19973069.79543/150‑1 (MQ=60)
                                                                                                                       GCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAA                                >  SRR19973069.2979754/1‑150 (MQ=60)
                                                                                                                       GCAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAA                                >  SRR19973069.2977585/1‑150 (MQ=60)
                                                                                                                        CAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAT                               <  SRR19973069.3176726/150‑1 (MQ=60)
                                                                                                                        CAGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAG                               <  SRR19973069.3176765/150‑1 (MQ=60)
                                                                                                                         AGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGATAAGC                              >  SRR19973069.313741/1‑150 (MQ=60)
                                                                                                                         AGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGC                              >  SRR19973069.1462702/1‑150 (MQ=60)
                                                                                                                         AGTGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGC                              >  SRR19973069.313948/1‑150 (MQ=60)
                                                                                                                           TGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTG                            <  SRR19973069.2770402/150‑1 (MQ=60)
                                                                                                                           TGGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTG                            <  SRR19973069.3541362/150‑1 (MQ=60)
                                                                                                                            GGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGA                           <  SRR19973069.1258087/150‑1 (MQ=60)
                                                                                                                            GGACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGA                           <  SRR19973069.1225586/150‑1 (MQ=60)
                                                                                                                             GACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAG                          <  SRR19973069.2489933/150‑1 (MQ=60)
                                                                                                                             GACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAG                          <  SRR19973069.558722/150‑1 (MQ=60)
                                                                                                                             GACGTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAG                          <  SRR19973069.2490200/150‑1 (MQ=60)
                                                                                                                                GTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTAC                       >  SRR19973069.2227956/1‑150 (MQ=60)
                                                                                                                                GTTCCTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTAC                       >  SRR19973069.313198/1‑150 (MQ=60)
                                                                                                                                    CTGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACA                   >  SRR19973069.2175798/1‑150 (MQ=60)
                                                                                                                                     TGTTACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAG                  <  SRR19973069.3286732/150‑1 (MQ=60)
                                                                                                                                        TACCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTAT               <  SRR19973069.1392495/150‑1 (MQ=60)
                                                                                                                                          CCCTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTC             <  SRR19973069.1447270/150‑1 (MQ=60)
                                                                                                                                            CTGAAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGG           >  SRR19973069.1248631/1‑150 (MQ=60)
                                                                                                                                               AAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCA        >  SRR19973069.2891672/1‑150 (MQ=60)
                                                                                                                                               AAGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCA        >  SRR19973069.967080/1‑150 (MQ=60)
                                                                                                                                                AGATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAG       <  SRR19973069.709504/150‑1 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     <  SRR19973069.1535205/150‑1 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     >  SRR19973069.3321325/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     >  SRR19973069.3321577/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     <  SRR19973069.3448571/150‑1 (MQ=60)
                                                                                                                                                  ATTAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTT     >  SRR19973069.3482422/1‑150 (MQ=60)
                                                                                                                                                    TAGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAA   >  SRR19973069.2793656/1‑150 (MQ=60)
                                                                                                                                                     AGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGTAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  <  SRR19973069.585673/150‑1 (MQ=60)
                                                                                                                                                     AGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  SRR19973069.741939/1‑150 (MQ=60)
                                                                                                                                                     AGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  SRR19973069.1564026/1‑150 (MQ=60)
                                                                                                                                                     AGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  SRR19973069.2287126/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAG  >  CP009273/3403910‑3404208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑