Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,738,431 C→A 100% P90T (CCA→ACA)  yiaN → 2,3‑diketo‑L‑gulonate TRAP transporter large permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,738,4310CA99.5% 607.4 / ‑4.0 186P90T (CCA→ACA) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (103/82);  total (104/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

GATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGG  >  CP009273/3738286‑3738575
                                                                                                                                                 |                                                                                                                                                
 aTCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAg                                                                                                                                               >  2:3273862/1‑148 (MQ=255)
 aTCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAg                                                                                                                                               >  2:1056973/1‑148 (MQ=255)
  tCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAgg                                                                                                                                              >  2:3087278/1‑148 (MQ=255)
            aaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctgggc                                                                                                                                    <  1:1361304/148‑1 (MQ=255)
            aaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctgggc                                                                                                                                    <  2:2627658/148‑1 (MQ=255)
            aaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctgggc                                                                                                                                    <  1:52115/148‑1 (MQ=255)
             aCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctgggct                                                                                                                                   >  1:1065466/1‑148 (MQ=255)
              cGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCGTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCGGGGCTa                                                                                                                                  >  1:886047/1‑148 (MQ=255)
                cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACg                                                                                                                                <  2:3263708/148‑1 (MQ=255)
                cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACg                                                                                                                                <  2:1521946/148‑1 (MQ=255)
                 tGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGt                                                                                                                               <  2:2893862/148‑1 (MQ=255)
                  ggTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTc                                                                                                                              <  2:1447235/148‑1 (MQ=255)
                    tGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCCAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCgg                                                                                                                            >  2:2894307/1‑148 (MQ=255)
                      aaCGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCg                                                                                                                          >  1:2102266/1‑148 (MQ=255)
                        cGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTg                                                                                                                        <  1:3028425/148‑1 (MQ=255)
                        cGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTg                                                                                                                        <  1:2347319/148‑1 (MQ=255)
                        cGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTg                                                                                                                        <  1:25928/148‑1 (MQ=255)
                        cGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTg                                                                                                                        <  1:1777262/148‑1 (MQ=255)
                           aGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTg                                                                                                                     >  1:1090755/1‑148 (MQ=255)
                            gCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTgg                                                                                                                    <  1:1734947/148‑1 (MQ=255)
                               gATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAg                                                                                                                 >  2:2009154/1‑148 (MQ=255)
                                 tAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt                                                                                                               >  2:2107937/1‑148 (MQ=255)
                                  aGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTa                                                                                                              >  2:2863467/1‑148 (MQ=255)
                                    cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATg                                                                                                            <  1:2242316/148‑1 (MQ=255)
                                     ttCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGa                                                                                                           <  1:936453/148‑1 (MQ=255)
                                      tctcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                                          <  1:3281623/148‑1 (MQ=255)
                                        tcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTa                                                                                                        <  2:884745/148‑1 (MQ=255)
                                         cccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCCTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAt                                                                                                       >  1:3168468/1‑148 (MQ=255)
                                          ccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATg                                                                                                      >  2:1005018/1‑148 (MQ=255)
                                          ccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATg                                                                                                      >  2:636632/1‑148 (MQ=255)
                                            tgctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGc                                                                                                    >  2:1649492/1‑148 (MQ=255)
                                               tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg                                                                                                 <  1:2252895/148‑1 (MQ=255)
                                               tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg                                                                                                 <  1:591538/148‑1 (MQ=255)
                                                 gCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGcc                                                                                               <  1:2248339/148‑1 (MQ=255)
                                                    aTTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCttt                                                                                            <  2:1464671/148‑1 (MQ=255)
                                                    aTTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCttt                                                                                            <  2:319222/148‑1 (MQ=255)
                                                    aTTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCttt                                                                                            <  1:1807697/148‑1 (MQ=255)
                                                     ttCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTc                                                                                           <  2:66952/148‑1 (MQ=255)
                                                      tCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCt                                                                                          >  1:626373/1‑148 (MQ=255)
                                                        cGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTgg                                                                                        <  2:2155042/148‑1 (MQ=255)
                                                         gTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGt                                                                                       <  1:603262/148‑1 (MQ=255)
                                                          ttCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGtt                                                                                      >  1:1460804/1‑148 (MQ=255)
                                                          ttCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGtt                                                                                      >  2:2427551/1‑148 (MQ=255)
                                                          ttCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGtt                                                                                      >  1:2883036/1‑148 (MQ=255)
                                                             tttGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTNGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTg                                                                                   <  1:1678280/148‑1 (MQ=255)
                                                             tttGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTg                                                                                   <  2:1398587/148‑1 (MQ=255)
                                                              ttGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGc                                                                                  >  2:2038712/1‑148 (MQ=255)
                                                              ttGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGc                                                                                  >  2:1296200/1‑148 (MQ=255)
                                                                   cTGGAGGGTGAAATCATGAATGCGGGAGGCTTGTCAAAACGCATTGTTGACCTTCAGATTAAACTGGTGGGGCATAAAACAGGCGGGATGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTg                                                                             <  2:2081348/148‑1 (MQ=255)
                                                                       cGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGa                                                                         <  2:2131874/148‑1 (MQ=255)
                                                                        gggTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGAt                                                                        <  1:335594/148‑1 (MQ=255)
                                                                        gggTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGAt                                                                        <  1:2002712/148‑1 (MQ=255)
                                                                                 cATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgcc                                                               >  2:603436/1‑148 (MQ=255)
                                                                                  aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgccg                                                              <  2:43250/148‑1 (MQ=255)
                                                                                   tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGt                                                             <  2:2927034/148‑1 (MQ=255)
                                                                                    gAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                            >  1:279771/1‑148 (MQ=255)
                                                                                     aaTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTCTGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTg                                                           <  2:2175254/148‑1 (MQ=255)
                                                                                     aaTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTg                                                           >  1:1997160/1‑148 (MQ=255)
                                                                                           ggcggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGctc                                                     >  2:1741546/1‑148 (MQ=255)
                                                                                             cggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTa                                                   <  1:1850695/148‑1 (MQ=255)
                                                                                              ggcTTGTCAAATCGCATTGTTGACCTGCCGATTAAACTGGTGGGGCATATAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTGTGCCGTTGCGGATACCGCCGCCGTTGCGGctcttc                                                  <  2:2886647/148‑3 (MQ=255)
                                                                                               gcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                                                 >  1:581985/1‑148 (MQ=255)
                                                                                                cTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTg                                                >  2:2741485/1‑148 (MQ=255)
                                                                                                cTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTg                                                >  1:790764/1‑148 (MQ=255)
                                                                                                 ttGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTgg                                               >  1:3303317/1‑148 (MQ=255)
                                                                                                     cAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGcc                                           >  2:2529576/1‑148 (MQ=255)
                                                                                                       aaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCga                                         >  1:2809583/1‑148 (MQ=255)
                                                                                                        aaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgat                                        >  2:2099569/1‑148 (MQ=255)
                                                                                                        aaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgat                                        >  2:1177371/1‑148 (MQ=255)
                                                                                                         aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                       >  2:2088701/1‑148 (MQ=255)
                                                                                                         aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                       <  1:822531/148‑1 (MQ=255)
                                                                                                         aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                       >  1:2307895/1‑148 (MQ=255)
                                                                                                         aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                       >  1:2288145/1‑148 (MQ=255)
                                                                                                         aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                       >  2:2552117/1‑148 (MQ=255)
                                                                                                            cATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGatgatg                                    <  2:1503067/148‑1 (MQ=255)
                                                                                                            cATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGatgatg                                    <  1:1056973/148‑1 (MQ=255)
                                                                                                              ttgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcg                                  >  2:478162/1‑148 (MQ=255)
                                                                                                              ttgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcg                                  >  2:1686623/1‑148 (MQ=255)
                                                                                                               tgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcgc                                 >  2:1306334/1‑148 (MQ=255)
                                                                                                               tgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcgc                                 <  1:2952971/148‑1 (MQ=255)
                                                                                                                     ccTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGcc                           >  2:253908/1‑148 (MQ=255)
                                                                                                                           gATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTAt                     <  1:2167413/148‑1 (MQ=255)
                                                                                                                           gATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTAt                     <  2:1084394/148‑1 (MQ=255)
                                                                                                                            aTGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                    >  2:3174855/1‑148 (MQ=255)
                                                                                                                            aTGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                    >  2:1782408/1‑148 (MQ=255)
                                                                                                                            aTGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                    <  2:2315395/148‑1 (MQ=255)
                                                                                                                            aTGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                    >  1:2212294/1‑148 (MQ=255)
                                                                                                                            aTGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                    >  1:2213471/1‑148 (MQ=255)
                                                                                                                            aTGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                    >  1:679555/1‑148 (MQ=255)
                                                                                                                               aaaCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCgg                 >  1:2072549/1‑148 (MQ=255)
                                                                                                                               aaaCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCgg                 >  1:2073035/1‑148 (MQ=255)
                                                                                                                                 aCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTc               >  1:1342274/1‑148 (MQ=255)
                                                                                                                                 aCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTc               >  1:3263376/1‑148 (MQ=255)
                                                                                                                                  cTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCa              >  2:3093669/1‑148 (MQ=255)
                                                                                                                                             tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcg   >  2:3124710/1‑148 (MQ=255)
                                                                                                                                             tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcg   <  2:2295630/148‑1 (MQ=255)
                                                                                                                                             tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcg   <  1:1008403/148‑1 (MQ=255)
                                                                                                                                             tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcg   >  1:3294838/1‑148 (MQ=255)
                                                                                                                                             tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcg   <  1:1005018/148‑1 (MQ=255)
                                                                                                                                              aaaaCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGcggcgg  >  2:814498/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGG  >  CP009273/3738286‑3738575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  >  CP009273/3738283‑3738580
                                                                                                                                                    |                                                                                                                                                     
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTACGTTCTTTGTGCTGGCGGGTGAAATAATGAATGCGGGCGGCTTGTCAAAAAGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAAC                                                                                                                                                      >  SRR19973070.2937207/1‑150 (MQ=60)
   GATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGG                                                                                                                                                   <  SRR19973070.2825001/150‑1 (MQ=60)
              AAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCT                                                                                                                                        >  SRR19973070.1072923/1‑150 (MQ=60)
              AAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCT                                                                                                                                        >  SRR19973070.1072927/1‑150 (MQ=60)
               AACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTA                                                                                                                                       <  SRR19973070.52744/150‑1 (MQ=60)
               AACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTA                                                                                                                                       <  SRR19973070.1370588/150‑1 (MQ=60)
               AACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCGTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCGGGGCTA                                                                                                                                       >  SRR19973070.892303/1‑150 (MQ=60)
                    TGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCG                                                                                                                                  >  SRR19973070.3326573/1‑150 (MQ=60)
                       TGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCG                                                                                                                               >  SRR19973070.2116374/1‑150 (MQ=60)
                          ACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGC                                                                                                                            >  SRR19973070.2706551/1‑150 (MQ=60)
                           CGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCT                                                                                                                           <  SRR19973070.1423786/150‑1 (MQ=60)
                           CGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCT                                                                                                                           <  SRR19973070.3047706/150‑1 (MQ=60)
                           CGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCT                                                                                                                           <  SRR19973070.26283/150‑1 (MQ=60)
                           CGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCT                                                                                                                           <  SRR19973070.2362888/150‑1 (MQ=60)
                           CGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCA                                                                                                                           <  SRR19973070.1789560/150‑1 (MQ=60)
                           CGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGGTCTTTGTGCTGGCGGGTGAAATCAAGAATGCGGGCGGCTTGTCAAAAAGCATTGTTGACCTGCCGATTAAACAGGTGGGGCCTAAAAAAGGCGGGCTGGGCTACGGCGGCGTGCT                                                                                                                           >  SRR19973070.1973979/1‑150 (MQ=60)
                            GGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTG                                                                                                                          >  SRR19973070.1098292/1‑150 (MQ=60)
                              AGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAAAGCATTGTTGACCTGCCGATGAAACTGGTGGGGCCTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGC                                                                                                                        >  SRR19973070.851441/1‑150 (MQ=60)
                               GCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCA                                                                                                                       <  SRR19973070.1746974/150‑1 (MQ=60)
                                 CGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGC                                                                                                                     >  SRR19973070.537479/1‑150 (MQ=60)
                                     AGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATAATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATG                                                                                                                 <  SRR19973070.2011094/150‑1 (MQ=60)
                                       CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAT                                                                                                               <  SRR19973070.2257123/150‑1 (MQ=60)
                                       CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAT                                                                                                               <  SRR19973070.2257386/150‑1 (MQ=60)
                                       CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAT                                                                                                               <  SRR19973070.2257124/150‑1 (MQ=60)
                                       CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAAATGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAT                                                                                                               >  SRR19973070.1360115/1‑150 (MQ=60)
                                        TTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATT                                                                                                              <  SRR19973070.943214/150‑1 (MQ=60)
                                        TTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATT                                                                                                              <  SRR19973070.943128/150‑1 (MQ=60)
                                         TCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTA                                                                                                             <  SRR19973070.3302700/150‑1 (MQ=60)
                                          CTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCCTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAT                                                                                                            >  SRR19973070.3188971/1‑150 (MQ=60)
                                            CCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGG                                                                                                          >  SRR19973070.518961/1‑150 (MQ=60)
                                              CTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCG                                                                                                        <  SRR19973070.2323208/150‑1 (MQ=60)
                                                GCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAG                                                                                                      >  SRR19973070.1046087/1‑150 (MQ=60)
                                                  TGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCC                                                                                                    <  SRR19973070.595630/150‑1 (MQ=60)
                                                  TGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCC                                                                                                    <  SRR19973070.2267823/150‑1 (MQ=60)
                                                    GCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT                                                                                                  <  SRR19973070.2263649/150‑1 (MQ=60)
                                                    GCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT                                                                                                  <  SRR19973070.2263211/150‑1 (MQ=60)
                                                     gctcttccgatcTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTT                                                                                                 <  SRR19973070.2900286/138‑1 (MQ=60)
                                                      GATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTC                                                                                                >  SRR19973070.1081201/1‑150 (MQ=60)
                                                       ATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCT                                                                                               <  SRR19973070.1820172/150‑1 (MQ=60)
                                                       ATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCT                                                                                               >  SRR19973070.630802/1‑150 (MQ=60)
                                                        TTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTG                                                                                              >  SRR19973070.628462/1‑150 (MQ=60)
                                                        TTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTG                                                                                              >  SRR19973070.2297643/1‑150 (MQ=60)
                                                           CGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTT                                                                                           >  SRR19973070.1471001/1‑150 (MQ=60)
                                                           CGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTT                                                                                           >  SRR19973070.2901699/1‑150 (MQ=60)
                                                            GTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTC                                                                                          <  SRR19973070.607472/150‑1 (MQ=60)
                                                              TCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTG                                                                                        <  SRR19973070.3307543/150‑1 (MQ=60)
                                                                TTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTNGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCC                                                                                      <  SRR19973070.1689767/150‑1 (MQ=60)
                                                                TTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTNGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCC                                                                                      <  SRR19973070.1689770/150‑1 (MQ=60)
                                                                      CTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCG                                                                                <  SRR19973070.721489/150‑1 (MQ=60)
                                                                           GGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAC                                                                           <  SRR19973070.338039/150‑1 (MQ=60)
                                                                           GGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAC                                                                           <  SRR19973070.2016272/150‑1 (MQ=60)
                                                                              TGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGC                                                                        >  SRR19973070.906342/1‑150 (MQ=60)
                                                                                     ATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTT                                                                 >  SRR19973070.281582/1‑150 (MQ=60)
                                                                                      TGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                                >  SRR19973070.2010691/1‑150 (MQ=60)
                                                                                                CGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACT                                                      >  SRR19973070.585994/1‑150 (MQ=60)
                                                                                                CGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACT                                                      <  SRR19973070.1862769/150‑1 (MQ=60)
                                                                                                CGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACT                                                      >  SRR19973070.674072/1‑150 (MQ=60)
                                                                                                CGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACT                                                      <  SRR19973070.1863377/150‑1 (MQ=60)
                                                                                                 GGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTG                                                     >  SRR19973070.796244/1‑150 (MQ=60)
                                                                                                  GCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGG                                                    >  SRR19973070.3324470/1‑150 (MQ=60)
                                                                                                      GTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCC                                                >  SRR19973070.1818952/1‑150 (MQ=60)
                                                                                                        CAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGA                                              >  SRR19973070.250114/1‑150 (MQ=60)
                                                                                                        CAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGA                                              >  SRR19973070.2827748/1‑150 (MQ=60)
                                                                                                          AAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATG                                            >  SRR19973070.2303362/1‑150 (MQ=60)
                                                                                                          AAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATG                                            >  SRR19973070.2323240/1‑150 (MQ=60)
                                                                                                            ACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGAT                                          <  SRR19973070.828074/150‑1 (MQ=60)
                                                                                                            ACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGAT                                          <  SRR19973070.828136/150‑1 (MQ=60)
                                                                                                               CATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCG                                       <  SRR19973070.1064397/150‑1 (MQ=60)
                                                                                                                  TGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAG                                    <  SRR19973070.2971974/150‑1 (MQ=60)
                                                                                                                          TGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACT                            <  SRR19973070.2192732/150‑1 (MQ=60)
                                                                                                                             CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                         >  SRR19973070.2226840/1‑150 (MQ=60)
                                                                                                                             CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                         >  SRR19973070.2227987/1‑150 (MQ=60)
                                                                                                                             CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                         >  SRR19973070.684399/1‑150 (MQ=60)
                                                                                                                             CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                         >  SRR19973070.2228019/1‑150 (MQ=60)
                                                                                                                             CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                         >  SRR19973070.3033134/1‑150 (MQ=60)
                                                                                                                              GATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCC                        <  SRR19973070.2181791/150‑1 (MQ=60)
                                                                                                                               ATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCG                       >  SRR19973070.1315340/1‑150 (MQ=60)
                                                                                                                                TGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGG                      >  SRR19973070.2086992/1‑150 (MQ=60)
                                                                                                                                TGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGG                      >  SRR19973070.2950045/1‑150 (MQ=60)
                                                                                                                                TGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGG                      >  SRR19973070.2086880/1‑150 (MQ=60)
                                                                                                                                TGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGG                      >  SRR19973070.587169/1‑150 (MQ=60)
                                                                                                                                TGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGG                      >  SRR19973070.2086504/1‑150 (MQ=60)
                                                                                                                                  AAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTC                    >  SRR19973070.3284381/1‑150 (MQ=60)
                                                                                                                                  AAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTC                    >  SRR19973070.1351490/1‑150 (MQ=60)
                                                                                                                                  AAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTC                    >  SRR19973070.3284657/1‑150 (MQ=60)
                                                                                                                                   AACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCA                   >  SRR19973070.2033395/1‑150 (MQ=60)
                                                                                                                                              CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG        >  SRR19973070.1782276/1‑150 (MQ=60)
                                                                                                                                              CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG        >  SRR19973070.3315976/1‑150 (MQ=60)
                                                                                                                                              CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG        >  SRR19973070.3315962/1‑150 (MQ=60)
                                                                                                                                              CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG        >  SRR19973070.3164698/1‑150 (MQ=60)
                                                                                                                                              CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG        >  SRR19973070.2136118/1‑150 (MQ=60)
                                                                                                                                                TAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGG      <  SRR19973070.1012182/150‑1 (MQ=60)
                                                                                                                                                TAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGG      <  SRR19973070.1015580/150‑1 (MQ=60)
                                                                                                                                                 AAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGG     >  SRR19973070.1969254/1‑150 (MQ=60)
                                                                                                                                                  AAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGC    >  SRR19973070.1704023/1‑150 (MQ=60)
                                                                                                                                                  AAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGC    >  SRR19973070.1619751/1‑150 (MQ=60)
                                                                                                                                                  AAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGC    >  SRR19973070.1619745/1‑150 (MQ=60)
                                                                                                                                                   AACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCT   >  SRR19973070.2695981/1‑150 (MQ=60)
                                                                                                                                                   AACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCT   >  SRR19973070.2291352/1‑150 (MQ=60)
                                                                                                                                                    ACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  <  SRR19973070.2022754/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  >  CP009273/3738283‑3738580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑