Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,738,431 C→A 100% P90T (CCA→ACA)  yiaN → 2,3‑diketo‑L‑gulonate TRAP transporter large permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,738,4310CA98.9% 631.9 / ‑1.8 195P90T (CCA→ACA) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (105/87);  total (106/89)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGG  >  CP009273/3738287‑3738575
                                                                                                                                                |                                                                                                                                                
aTCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAg                                                                                                                                               >  1:3327023/1‑148 (MQ=255)
 tCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAgg                                                                                                                                              <  2:2233682/148‑1 (MQ=255)
 tCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAgg                                                                                                                                              >  2:3255286/1‑148 (MQ=255)
  cATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGc                                                                                                                                             <  1:24044/148‑1 (MQ=255)
  cATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGc                                                                                                                                             >  1:2323430/1‑148 (MQ=255)
        acaAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGTAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctg                                                                                                                                       >  1:721274/1‑148 (MQ=255)
        acaAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctg                                                                                                                                       >  1:290874/1‑148 (MQ=255)
         caAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGGTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCGTGTCAAAACGCATTGTTGACCTGCCGATGTAACTGGTGGGGCCTAAAACAGGCgggctgg                                                                                                                                      >  1:3266908/1‑148 (MQ=255)
          aaaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTGTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctggg                                                                                                                                     >  1:1011757/1‑148 (MQ=255)
          aaaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctggg                                                                                                                                     >  1:1011884/1‑148 (MQ=255)
           aaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctgggc                                                                                                                                    <  2:2585832/148‑1 (MQ=255)
             cGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTGTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTa                                                                                                                                  >  1:2722640/1‑148 (MQ=255)
             cGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTa                                                                                                                                  >  2:1996453/1‑148 (MQ=255)
               cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACg                                                                                                                                <  2:3119556/148‑1 (MQ=255)
               cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACg                                                                                                                                <  2:2803958/148‑1 (MQ=255)
               cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACg                                                                                                                                <  1:274863/148‑1 (MQ=255)
                  gTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCg                                                                                                                             >  2:2009208/1‑148 (MQ=255)
                       cGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTg                                                                                                                        >  2:1159057/1‑148 (MQ=255)
                        ggAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGc                                                                                                                       <  2:313915/148‑1 (MQ=255)
                               aTAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGc                                                                                                                <  2:2881088/148‑1 (MQ=255)
                               aTAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGc                                                                                                                <  2:1130850/148‑1 (MQ=255)
                               aTAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGc                                                                                                                >  1:2461821/1‑148 (MQ=255)
                                tAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt                                                                                                               <  2:1853445/148‑1 (MQ=255)
                                 aGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTa                                                                                                              >  1:2040491/1‑148 (MQ=255)
                                 aGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTa                                                                                                              >  2:2049991/1‑148 (MQ=255)
                                 aGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTa                                                                                                              >  2:3197320/1‑148 (MQ=255)
                                  gCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTAt                                                                                                             >  1:1437267/1‑148 (MQ=255)
                                  gCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTAt                                                                                                             <  2:23318/148‑1 (MQ=255)
                                  gCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTAt                                                                                                             <  2:23251/148‑1 (MQ=255)
                                   cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATg                                                                                                            <  1:3362525/148‑1 (MQ=255)
                                     tctcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                                          <  1:1111179/148‑1 (MQ=255)
                                     tctcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                                          <  1:2861789/148‑1 (MQ=255)
                                       tcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTa                                                                                                        <  1:1141597/148‑1 (MQ=255)
                                        cccTGCTGGCGATTCCGTTCTTTTTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTTACCTGCCGATGAAACTGGTGGGGCATAAAACATGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAt                                                                                                       >  1:2077298/1‑148 (MQ=255)
                                        cccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTTTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAt                                                                                                       <  2:293645/148‑1 (MQ=255)
                                              tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGTTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg                                                                                                 <  2:2951808/148‑1 (MQ=255)
                                              tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg                                                                                                 <  1:954925/148‑1 (MQ=255)
                                              tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg                                                                                                 <  2:3081668/148‑1 (MQ=255)
                                              tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg                                                                                                 <  2:2951472/148‑1 (MQ=255)
                                               ggCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGc                                                                                                <  1:2712440/148‑1 (MQ=255)
                                                gCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGcc                                                                                               <  1:764751/148‑1 (MQ=255)
                                                 cGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCt                                                                                              <  2:1792626/148‑1 (MQ=255)
                                                     tCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCt                                                                                          <  1:2342696/148‑1 (MQ=255)
                                                     tCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCt                                                                                          >  1:37318/1‑148 (MQ=255)
                                                        gTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGt                                                                                       <  1:3032876/148‑1 (MQ=255)
                                                         ttCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGtt                                                                                      <  1:1846101/148‑1 (MQ=255)
                                                         ttCTTTGTGCTGGCGGGTGAAATCAGGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGtt                                                                                      <  2:2384918/148‑1 (MQ=255)
                                                          tCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTc                                                                                     >  1:1659482/1‑148 (MQ=255)
                                                             ttGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGc                                                                                  <  1:3420423/148‑1 (MQ=255)
                                                                  cTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTg                                                                             <  2:1893717/148‑1 (MQ=255)
                                                                  cTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTg                                                                             >  1:3124066/1‑148 (MQ=255)
                                                                     gCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGGTCTGCCGTTGCgg                                                                          >  2:3435087/1‑148 (MQ=255)
                                                                        ggTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCAGGTTCTGCCGTTGCGGATa                                                                       <  2:52879/148‑1 (MQ=255)
                                                                          tGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAcc                                                                     >  2:1605271/1‑148 (MQ=255)
                                                                           gAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccg                                                                    >  2:103172/1‑148 (MQ=255)
                                                                           gAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAACTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccg                                                                    >  2:720470/1‑148 (MQ=255)
                                                                            aaaTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgc                                                                   >  1:1963972/1‑148 (MQ=255)
                                                                            aaaTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgc                                                                   >  2:132484/1‑148 (MQ=255)
                                                                             aaTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgcc                                                                  >  1:1414883/1‑148 (MQ=255)
                                                                              aTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccg                                                                 >  1:304588/1‑148 (MQ=255)
                                                                               tCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgc                                                                >  2:671658/1‑148 (MQ=255)
                                                                                 aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgctgccg                                                              >  1:238523/1‑148 (MQ=255)
                                                                                 aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgccg                                                              >  1:237861/1‑148 (MQ=255)
                                                                                 aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgccg                                                              <  1:1809348/148‑1 (MQ=255)
                                                                                 aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgccg                                                              >  2:2989994/1‑148 (MQ=255)
                                                                                  tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGt                                                             >  2:1786720/1‑148 (MQ=255)
                                                                                  tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGt                                                             >  1:2431315/1‑148 (MQ=255)
                                                                                  tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGt                                                             <  1:824493/148‑1 (MQ=255)
                                                                                  tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGt                                                             >  1:67732/1‑148 (MQ=255)
                                                                                     aTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGc                                                          >  2:1527774/1‑148 (MQ=255)
                                                                                     aTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGc                                                          >  2:2810620/1‑148 (MQ=255)
                                                                                          ggcggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGctc                                                     >  2:23519/1‑148 (MQ=255)
                                                                                              gcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                                                 >  1:641011/1‑148 (MQ=255)
                                                                                              gcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                                                 >  2:208663/1‑148 (MQ=255)
                                                                                               cTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTg                                                <  1:2303084/148‑1 (MQ=255)
                                                                                               cTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTg                                                <  1:537348/148‑1 (MQ=255)
                                                                                                     aaaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCg                                          <  1:1302450/148‑1 (MQ=255)
                                                                                                      aaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCga                                         <  2:291632/148‑1 (MQ=255)
                                                                                                      aaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCga                                         <  2:290874/148‑1 (MQ=255)
                                                                                                        aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                       <  2:927089/148‑1 (MQ=255)
                                                                                                         cGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatga                                      >  2:1457875/1‑148 (MQ=255)
                                                                                                         cGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatga                                      <  1:3043529/148‑1 (MQ=255)
                                                                                                          gCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatgat                                     <  2:789335/148‑1 (MQ=255)
                                                                                                             ttgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcg                                  >  1:2014676/1‑148 (MQ=255)
                                                                                                                   aCCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAgcgc                            >  2:3297641/1‑148 (MQ=255)
                                                                                                                         cGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGACGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATTCCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTa                      <  1:3228433/148‑1 (MQ=255)
                                                                                                                          gATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTAt                     <  1:3435087/148‑1 (MQ=255)
                                                                                                                              aaaCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCgg                 <  1:1688821/148‑1 (MQ=255)
                                                                                                                                aCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTc               >  2:2601697/1‑148 (MQ=255)
                                                                                                                                aCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTc               <  1:2278644/148‑1 (MQ=255)
                                                                                                                                   ggtggGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAAc            <  1:906054/148‑1 (MQ=255)
                                                                                                                                   gggggggCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAAc            <  1:1928083/145‑1 (MQ=255)
                                                                                                                                    gtggGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAAcc           >  1:790805/1‑148 (MQ=255)
                                                                                                                                       gggCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCggg        >  2:27391/1‑148 (MQ=255)
                                                                                                                                       gggCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCggg        <  2:429489/148‑1 (MQ=255)
                                                                                                                                          cATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGggcgg     >  1:3256681/1‑148 (MQ=255)
                                                                                                                                           aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGggcggc    >  2:2843064/1‑148 (MQ=255)
                                                                                                                                            tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcg   >  1:3225139/1‑148 (MQ=255)
                                                                                                                                            tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcg   >  1:1534881/1‑148 (MQ=255)
                                                                                                                                             aaaaCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGcggcgg  <  1:2367382/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGG  >  CP009273/3738287‑3738575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCT  >  CP009273/3738285‑3738579
                                                                                                                                                  |                                                                                                                                                    
AGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAG                                                                                                                                                   <  SRR19973065.1196430/150‑1 (MQ=60)
AGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAG                                                                                                                                                   >  SRR19973065.3347324/1‑150 (MQ=60)
  ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGC                                                                                                                                                 >  SRR19973065.2337982/1‑150 (MQ=60)
    CATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGG                                                                                                                                               <  SRR19973065.24365/150‑1 (MQ=60)
        GCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGTAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTG                                                                                                                                           >  SRR19973065.726100/1‑150 (MQ=60)
        GCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTTACCTGCCGATGAAACAGGTGGGGCATAAAACATGCGGGCTG                                                                                                                                           >  SRR19973065.293524/1‑150 (MQ=60)
        GCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTG                                                                                                                                           <  SRR19973065.602994/150‑1 (MQ=60)
        GCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTG                                                                                                                                           >  SRR19973065.292766/1‑150 (MQ=60)
         CACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATAATTAATGCGGGCGGCGTTTCAAAACGCATTGTTGACCTGCCGATGAAACGGGTGGGGCCTAAAACAGGCGGGCTGG                                                                                                                                          >  SRR19973065.3286255/1‑150 (MQ=60)
         CACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGGTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCGTGTCAAAACGCATTGTTGACCTGCCGATGTAACTGGTGGGGCCTAAAACAGGCGGGCTGG                                                                                                                                          >  SRR19973065.3286714/1‑150 (MQ=60)
          ACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTGTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGG                                                                                                                                         >  SRR19973065.1018466/1‑150 (MQ=60)
          ACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGG                                                                                                                                         >  SRR19973065.1018594/1‑150 (MQ=60)
             AACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTGTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTTTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTA                                                                                                                                      >  SRR19973065.2739576/1‑150 (MQ=60)
               CGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCGGGGCTACG                                                                                                                                    >  SRR19973065.3380393/1‑150 (MQ=60)
                 CTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTC                                                                                                                                  <  SRR19973065.276707/150‑1 (MQ=60)
                         CGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCT                                                                                                                          <  SRR19973065.3345203/150‑1 (MQ=60)
                               CGATAGCTTCTCCCTGCTGGCGATTCCGTTGTTTGTGCTTGCGGGTGAAATAATTAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGTAACTGGTGGGGCATAAAAAAAGCGGGCTGGGCTACGTCGGCGTGCTGGCAGC                                                                                                                    >  SRR19973065.2477332/1‑150 (MQ=60)
                               CGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGC                                                                                                                    >  SRR19973065.2750244/1‑150 (MQ=60)
                               CGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGC                                                                                                                    >  SRR19973065.2477337/1‑150 (MQ=60)
                                 ATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTA                                                                                                                  >  SRR19973065.2053556/1‑150 (MQ=60)
                                 ATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTA                                                                                                                  <  SRR19973065.2838855/150‑1 (MQ=60)
                                  TAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTAT                                                                                                                 >  SRR19973065.1446591/1‑150 (MQ=60)
                                   AGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATG                                                                                                                <  SRR19973065.1634518/150‑1 (MQ=60)
                                   AGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATG                                                                                                                >  SRR19973065.794566/1‑150 (MQ=60)
                                    GCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGA                                                                                                               <  SRR19973065.3106151/150‑1 (MQ=60)
                                    GCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGA                                                                                                               <  SRR19973065.1352805/150‑1 (MQ=60)
                                     CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAT                                                                                                              <  SRR19973065.3383082/150‑1 (MQ=60)
                                       TCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTA                                                                                                            <  SRR19973065.2879358/150‑1 (MQ=60)
                                       TCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTA                                                                                                            <  SRR19973065.1118444/150‑1 (MQ=60)
                                        CTCCCTGCTGGCGATTCCGTTCTTTTTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTTACCTGCCGATGAAACTGGTGGGGCATAAAACATGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAT                                                                                                           >  SRR19973065.2090580/1‑150 (MQ=60)
                                        CTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAT                                                                                                           >  SRR19973065.2621603/1‑150 (MQ=60)
                                         TCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATG                                                                                                          <  SRR19973065.1149097/150‑1 (MQ=60)
                                         TCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATG                                                                                                          <  SRR19973065.1148974/150‑1 (MQ=60)
                                         TCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATG                                                                                                          <  SRR19973065.1148925/150‑1 (MQ=60)
                                          CCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGG                                                                                                         >  SRR19973065.1706088/1‑150 (MQ=60)
                                                TGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCC                                                                                                   <  SRR19973065.961238/150‑1 (MQ=60)
                                                 GGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCT                                                                                                  <  SRR19973065.2729334/150‑1 (MQ=60)
                                                 GGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCT                                                                                                  <  SRR19973065.2729300/150‑1 (MQ=60)
                                                 GGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCT                                                                                                  <  SRR19973065.2349425/150‑1 (MQ=60)
                                                  GCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT                                                                                                 <  SRR19973065.769837/150‑1 (MQ=60)
                                                  GCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT                                                                                                 <  SRR19973065.769695/150‑1 (MQ=60)
                                                   CGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTT                                                                                                <  SRR19973065.1689166/150‑1 (MQ=60)
                                                     ATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCT                                                                                              >  SRR19973065.37776/1‑150 (MQ=60)
                                                     ATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCT                                                                                              >  SRR19973065.1828518/1‑150 (MQ=60)
                                                       TCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGG                                                                                            <  SRR19973065.2357317/150‑1 (MQ=60)
                                                       TCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGG                                                                                            >  SRR19973065.1441602/1‑150 (MQ=60)
                                                       TCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGG                                                                                            <  SRR19973065.699417/150‑1 (MQ=60)
                                                          GTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTC                                                                                         <  SRR19973065.2045373/150‑1 (MQ=60)
                                                          GTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTC                                                                                         <  SRR19973065.3051472/150‑1 (MQ=60)
                                                          GTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTC                                                                                         <  SRR19973065.3051453/150‑1 (MQ=60)
                                                          GTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTC                                                                                         >  SRR19973065.1670600/1‑150 (MQ=60)
                                                          GTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTC                                                                                         >  SRR19973065.3420739/1‑150 (MQ=60)
                                                           TTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCT                                                                                        <  SRR19973065.1858267/150‑1 (MQ=60)
                                                               TTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCG                                                                                    <  SRR19973065.3441318/150‑1 (MQ=60)
                                                                  TGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTG                                                                                 >  SRR19973065.3143090/1‑150 (MQ=60)
                                                                      GGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGA                                                                             <  SRR19973065.3203260/150‑1 (MQ=60)
                                                                            TGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGC                                                                       >  SRR19973065.1976795/1‑150 (MQ=60)
                                                                             GAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCC                                                                      >  SRR19973065.1424125/1‑150 (MQ=60)
                                                                              AAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCG                                                                     >  SRR19973065.2308627/1‑150 (MQ=60)
                                                                              AAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCG                                                                     >  SRR19973065.533262/1‑150 (MQ=60)
                                                                              AAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCG                                                                     >  SRR19973065.306605/1‑150 (MQ=60)
                                                                                 TCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCTGCCG                                                                  >  SRR19973065.240357/1‑150 (MQ=60)
                                                                                 TCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCTGCCG                                                                  >  SRR19973065.240234/1‑150 (MQ=60)
                                                                                 TCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCG                                                                  >  SRR19973065.239723/1‑150 (MQ=60)
                                                                                 TCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCG                                                                  >  SRR19973065.239569/1‑150 (MQ=60)
                                                                                  CATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGT                                                                 >  SRR19973065.68449/1‑150 (MQ=60)
                                                                                  CATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGT                                                                 >  SRR19973065.2446653/1‑150 (MQ=60)
                                                                                  CATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGT                                                                 >  SRR19973065.2446235/1‑150 (MQ=60)
                                                                                  CATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGT                                                                 >  SRR19973065.673544/1‑150 (MQ=60)
                                                                                   ATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTT                                                                <  SRR19973065.1821254/150‑1 (MQ=60)
                                                                                    TGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                               <  SRR19973065.829902/150‑1 (MQ=60)
                                                                                    TGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                               <  SRR19973065.830205/150‑1 (MQ=60)
                                                                                    TGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                               <  SRR19973065.829553/150‑1 (MQ=60)
                                                                                       ATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCG                                                            >  SRR19973065.3272845/1‑150 (MQ=60)
                                                                                              CGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACT                                                     >  SRR19973065.645217/1‑150 (MQ=60)
                                                                                                 CTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGT                                                  <  SRR19973065.540846/150‑1 (MQ=60)
                                                                                                 CTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGT                                                  >  SRR19973065.2912514/1‑150 (MQ=60)
                                                                                                 CTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGT                                                  <  SRR19973065.2317568/150‑1 (MQ=60)
                                                                                                 CTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGT                                                  >  SRR19973065.2391600/1‑150 (MQ=60)
                                                                                                 CTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGT                                                  <  SRR19973065.2317580/150‑1 (MQ=60)
                                                                                                      CAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGA                                             >  SRR19973065.2074721/1‑150 (MQ=60)
                                                                                                       AAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGAT                                            <  SRR19973065.1311026/150‑1 (MQ=60)
                                                                                                       AAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGAT                                            >  SRR19973065.1532713/1‑150 (MQ=60)
                                                                                                        AAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATG                                           >  SRR19973065.922043/1‑150 (MQ=60)
                                                                                                           CGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATG                                        <  SRR19973065.3062156/150‑1 (MQ=60)
                                                                                                             CATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCG                                      >  SRR19973065.2027662/1‑150 (MQ=60)
                                                                                                                    GACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCC                               >  SRR19973065.2286816/1‑150 (MQ=60)
                                                                                                                     ACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCA                              <  SRR19973065.1104573/150‑1 (MQ=60)
                                                                                                                        TGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACT                           <  SRR19973065.1986059/150‑1 (MQ=60)
                                                                                                                           CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                        <  SRR19973065.3247748/150‑1 (MQ=60)
                                                                                                                           CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGACGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATTCCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                        <  SRR19973065.3247810/150‑1 (MQ=60)
                                                                                                                            GATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCC                       <  SRR19973065.3456044/150‑1 (MQ=60)
                                                                                                                             ATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCG                      >  SRR19973065.372828/1‑150 (MQ=60)
                                                                                                                                AAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTC                   <  SRR19973065.1700098/150‑1 (MQ=60)
                                                                                                                                AAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTC                   <  SRR19973065.3447306/150‑1 (MQ=60)
                                                                                                                                  ACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAA                 <  SRR19973065.2293017/150‑1 (MQ=60)
                                                                                                                                  ACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAA                 <  SRR19973065.1866930/150‑1 (MQ=60)
                                                                                                                                    TGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACC               >  SRR19973065.796045/1‑150 (MQ=60)
                                                                                                                                     GGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCG              <  SRR19973065.1940899/150‑1 (MQ=60)
                                                                                                                                     GGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCG              <  SRR19973065.911907/150‑1 (MQ=60)
                                                                                                                                     GGGGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCG              <  SRR19973065.1940717/150‑1 (MQ=60)
                                                                                                                                          GGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGG         >  SRR19973065.3276382/1‑150 (MQ=60)
                                                                                                                                            CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG       >  SRR19973065.1545008/1‑150 (MQ=60)
                                                                                                                                            CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG       >  SRR19973065.3244505/1‑150 (MQ=60)
                                                                                                                                               AAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGG    <  SRR19973065.2382131/150‑1 (MQ=60)
                                                                                                                                               AAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGG    <  SRR19973065.2382132/150‑1 (MQ=60)
                                                                                                                                               AAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGG    >  SRR19973065.1181830/1‑150 (MQ=60)
                                                                                                                                                 AACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCT  <  SRR19973065.3400249/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
AGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCT  >  CP009273/3738285‑3738579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑