Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,738,431 C→A 100% P90T (CCA→ACA)  yiaN → 2,3‑diketo‑L‑gulonate TRAP transporter large permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,738,4310CA99.6% 897.4 / ‑4.2 254P90T (CCA→ACA) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (139/114);  total (139/115)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 255 total aligned reads displayed.

CAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGG  >  CP009273/3738284‑3738577
                                                                                                                                                   |                                                                                                                                                  
cAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACa                                                                                                                                                  <  1:3201606/150‑1 (MQ=255)
cAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACa                                                                                                                                                  >  1:3960853/1‑150 (MQ=255)
cAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACa                                                                                                                                                  >  2:3906071/1‑150 (MQ=255)
 aGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAg                                                                                                                                                 >  1:2969665/1‑150 (MQ=255)
  taTCATGGCACAAACGCGGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCTTTATTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAgg                                                                                                                                                <  1:3581676/149‑1 (MQ=255)
  gATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAgg                                                                                                                                                >  1:2678484/1‑150 (MQ=255)
     cATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgg                                                                                                                                             <  2:322370/150‑1 (MQ=255)
     cATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgg                                                                                                                                             >  2:2141087/1‑150 (MQ=255)
      aTGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCggg                                                                                                                                            >  2:453426/1‑150 (MQ=255)
              aaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTa                                                                                                                                    <  2:2445547/150‑1 (MQ=255)
                cGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACg                                                                                                                                  <  1:3472123/150‑1 (MQ=255)
                  cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATTATTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAAAAGGCGGGCTGGGCTACGTc                                                                                                                                >  1:539430/1‑150 (MQ=255)
                  cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTc                                                                                                                                <  1:3270395/150‑1 (MQ=255)
                  cTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTc                                                                                                                                >  1:630868/1‑150 (MQ=255)
                   tGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCg                                                                                                                               <  2:759340/150‑1 (MQ=255)
                   tGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCg                                                                                                                               >  1:1099900/1‑150 (MQ=255)
                   tGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCg                                                                                                                               <  1:1652194/150‑1 (MQ=255)
                    ggTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTACGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAAGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCgg                                                                                                                              >  1:4022161/1‑150 (MQ=255)
                      tGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCg                                                                                                                            >  1:865103/1‑150 (MQ=255)
                       gAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGt                                                                                                                           >  1:3067976/1‑150 (MQ=255)
                        aaCGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTg                                                                                                                          <  2:1691857/150‑1 (MQ=255)
                           ggAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTg                                                                                                                       >  1:1018660/1‑150 (MQ=255)
                           ggAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTg                                                                                                                       >  1:91067/1‑150 (MQ=255)
                               ccGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAg                                                                                                                   <  1:279610/150‑1 (MQ=255)
                                 gATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGACTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt                                                                                                                 <  2:1849719/150‑1 (MQ=255)
                                 gATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt                                                                                                                 >  2:2114860/1‑150 (MQ=255)
                                      cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                                            <  2:3034284/150‑1 (MQ=255)
                                      cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                                            <  1:1200489/150‑1 (MQ=255)
                                      cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                                            <  2:1760112/150‑1 (MQ=255)
                                       ttCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAtt                                                                                                           <  2:2250793/150‑1 (MQ=255)
                                         ctcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAt                                                                                                         >  2:2565489/1‑150 (MQ=255)
                                          tcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATg                                                                                                        >  1:1170212/1‑150 (MQ=255)
                                            ccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGc                                                                                                      <  2:4050895/150‑1 (MQ=255)
                                            ccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGc                                                                                                      <  1:1878040/150‑1 (MQ=255)
                                             ctgctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCg                                                                                                     <  2:2375846/150‑1 (MQ=255)
                                              tgctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGa                                                                                                    <  2:3388997/150‑1 (MQ=255)
                                                 tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGcc                                                                                                 >  2:3445174/1‑150 (MQ=255)
                                                 tgGCGAGTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGcc                                                                                                 <  1:2833367/150‑1 (MQ=255)
                                                    cGATTCCGTTCTTTGTGCTGGCGGGTGAAAACATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCttt                                                                                              >  1:3861569/1‑150 (MQ=255)
                                                      aTTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCt                                                                                            <  1:4153914/150‑1 (MQ=255)
                                                        tCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTgg                                                                                          <  2:2671378/150‑1 (MQ=255)
                                                        tCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTgg                                                                                          <  1:2622415/150‑1 (MQ=255)
                                                          cGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGTGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGtt                                                                                        >  2:803748/1‑150 (MQ=255)
                                                           gTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTc                                                                                       >  2:3676283/1‑150 (MQ=255)
                                                           gTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTc                                                                                       >  2:3695323/1‑150 (MQ=255)
                                                           gTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTc                                                                                       >  2:1456253/1‑150 (MQ=255)
                                                            ttCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCt                                                                                      >  2:4110942/1‑150 (MQ=255)
                                                             tCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCANCTATGATTATGGCGAGCCTTTCTGGTTCTg                                                                                     <  1:3852174/150‑1 (MQ=255)
                                                             tCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTg                                                                                     <  2:3540536/150‑1 (MQ=255)
                                                                   tgCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTg                                                                               >  2:719696/1‑150 (MQ=255)
                                                                     cTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCg                                                                             <  1:3168932/150‑1 (MQ=255)
                                                                      tGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCgg                                                                            <  1:1062284/150‑1 (MQ=255)
                                                                       ggCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGa                                                                           <  2:759746/150‑1 (MQ=255)
                                                                         cGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATa                                                                         >  2:1017926/1‑150 (MQ=255)
                                                                           ggTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAcc                                                                       <  2:2029962/150‑1 (MQ=255)
                                                                              gAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgcc                                                                    >  1:424756/1‑150 (MQ=255)
                                                                                aaTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgc                                                                  >  1:982992/1‑150 (MQ=255)
                                                                                 aTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgcc                                                                 <  2:1411332/150‑1 (MQ=255)
                                                                                  tCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgccg                                                                >  2:3285994/1‑150 (MQ=255)
                                                                                   cATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGt                                                               >  1:1684586/1‑150 (MQ=255)
                                                                                    aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                              <  1:1949117/150‑1 (MQ=255)
                                                                                    aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                              <  2:1860089/150‑1 (MQ=255)
                                                                                     tGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTg                                                             >  1:1547326/1‑150 (MQ=255)
                                                                                     tGAATGCGGGCGGCTTGTCAAAACGCAGTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTg                                                             <  1:3849529/150‑1 (MQ=255)
                                                                                       actGCGGGCGGCTTGTCTAATCGCATTGTTGACCTGCCGATGAAACTGGTGGGTCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGcc                                                           <  1:3683553/148‑1 (MQ=255)
                                                                                            gggcggCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGctct                                                      <  2:1169951/150‑1 (MQ=255)
                                                                                               cggcTTGTCAAAACACATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                                                   <  1:2319953/150‑1 (MQ=255)
                                                                                                ggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTg                                                  >  2:3338313/1‑150 (MQ=255)
                                                                                                   ttGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTg                                               <  1:1743493/150‑1 (MQ=255)
                                                                                                   ttGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTg                                               <  2:3786787/150‑1 (MQ=255)
                                                                                                      tCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCg                                            >  2:3381622/1‑150 (MQ=255)
                                                                                                       cAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCga                                           >  2:2327285/1‑150 (MQ=255)
                                                                                                       cAAAACGCATTGTTGACCAGCCGATGAAACTGGTGGTGCATAAAACAGTCGTGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTGACTGGTACTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACGGGTGCCga                                           >  2:1985070/1‑150 (MQ=255)
                                                                                                        aaaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgat                                          >  1:3424006/1‑150 (MQ=255)
                                                                                                         aaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                         >  1:1124336/1‑150 (MQ=255)
                                                                                                         aaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                         <  2:3952323/150‑1 (MQ=255)
                                                                                                              cATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcg                                    >  2:3702816/1‑150 (MQ=255)
                                                                                                               aTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcgc                                   >  1:1309259/1‑150 (MQ=255)
                                                                                                                ttgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCa                                  <  2:28092/150‑1 (MQ=255)
                                                                                                                     gACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGcc                             >  1:2446619/1‑150 (MQ=255)
                                                                                                                     gACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGcc                             >  1:1744037/1‑150 (MQ=255)
                                                                                                                      aCCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGTTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCa                            <  1:1309434/150‑1 (MQ=255)
                                                                                                                      aCCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCa                            >  2:55710/1‑150 (MQ=255)
                                                                                                                        cTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAAc                          >  1:2446107/1‑150 (MQ=255)
                                                                                                                         tGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACt                         <  1:2229831/150‑1 (MQ=255)
                                                                                                                            cGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                      >  2:2517859/1‑150 (MQ=255)
                                                                                                                            cGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                      >  2:1633881/1‑150 (MQ=255)
                                                                                                                            cGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGATGCCGCTCTACTGGTGCCGATGAAGCGCAGCGCCAACTATc                      >  1:2932992/1‑150 (MQ=255)
                                                                                                                              aTGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCg                    <  2:3396020/150‑1 (MQ=255)
                                                                                                                              aTGAAACTGGAGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCg                    >  2:1433853/1‑150 (MQ=255)
                                                                                                                                  aaCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCa                >  2:1089917/1‑150 (MQ=255)
                                                                                                                                  aaCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCa                >  2:1401399/1‑150 (MQ=255)
                                                                                                                                  aaCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCa                >  2:1401385/1‑150 (MQ=255)
                                                                                                                                      ggtggGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCg            >  1:1428922/1‑150 (MQ=255)
                                                                                                                                        tggGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCggg          >  2:3527762/1‑150 (MQ=255)
                                                                                                                                              aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTCTCCGGTCAACCGGGcggcgg    >  2:1513949/1‑150 (MQ=255)
                                                                                                                                              aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGcggcgg    <  1:2212985/150‑1 (MQ=255)
                                                                                                                                              aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGcggcgg    >  1:3264094/1‑150 (MQ=255)
                                                                                                                                               tAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCggg   >  2:1243727/1‑150 (MQ=255)
                                                                                                                                                aaaaCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCgggg  >  1:1389739/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGG  >  CP009273/3738284‑3738577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  >  CP009273/3738283‑3738580
                                                                                                                                                    |                                                                                                                                                     
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAAC                                                                                                                                                      <  SRR19973074.982021/150‑1 (MQ=60)
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAAC                                                                                                                                                      >  SRR19973074.2063091/1‑150 (MQ=60)
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAAC                                                                                                                                                      >  SRR19973074.2063076/1‑150 (MQ=60)
 CAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACA                                                                                                                                                     <  SRR19973074.3207494/150‑1 (MQ=60)
 CAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACA                                                                                                                                                     <  SRR19973074.3207510/150‑1 (MQ=60)
 CAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACA                                                                                                                                                     >  SRR19973074.3968153/1‑150 (MQ=60)
  AGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAG                                                                                                                                                    >  SRR19973074.2975180/1‑150 (MQ=60)
   TATCATGGCACAAACGCGGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCTTTATTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGG                                                                                                                                                   <  SRR19973074.3588282/150‑1 (MQ=60)
   GATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGG                                                                                                                                                   >  SRR19973074.2683466/1‑150 (MQ=60)
    ATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGC                                                                                                                                                  >  SRR19973074.1772980/1‑150 (MQ=60)
       ATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGG                                                                                                                                               >  SRR19973074.3220005/1‑150 (MQ=60)
           CACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGG                                                                                                                                           <  SRR19973074.1151822/150‑1 (MQ=60)
           CACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGG                                                                                                                                           >  SRR19973074.4035021/1‑150 (MQ=60)
               AACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTA                                                                                                                                       >  SRR19973074.2437131/1‑150 (MQ=60)
               AACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTA                                                                                                                                       <  SRR19973074.2274364/150‑1 (MQ=60)
                 CGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACG                                                                                                                                     <  SRR19973074.3478502/150‑1 (MQ=60)
                 CGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACG                                                                                                                                     <  SRR19973074.3534398/150‑1 (MQ=60)
                   CTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATTATTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAAAAGGCGGGCTGGGCTACGTC                                                                                                                                   >  SRR19973074.540507/1‑150 (MQ=60)
                   CTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTC                                                                                                                                   >  SRR19973074.632051/1‑150 (MQ=60)
                   CTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTC                                                                                                                                   <  SRR19973074.3276461/150‑1 (MQ=60)
                    TGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCG                                                                                                                                  <  SRR19973074.1655313/150‑1 (MQ=60)
                    TGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCG                                                                                                                                  >  SRR19973074.1101961/1‑150 (MQ=60)
                     GGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGG                                                                                                                                 >  SRR19973074.4029737/1‑150 (MQ=60)
                     GGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTACGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAAGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGG                                                                                                                                 >  SRR19973074.4029594/1‑150 (MQ=60)
                       TGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCG                                                                                                                               >  SRR19973074.866771/1‑150 (MQ=60)
                        GAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGT                                                                                                                              <  SRR19973074.1959321/150‑1 (MQ=60)
                        GAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGT                                                                                                                              >  SRR19973074.3073626/1‑150 (MQ=60)
                            GGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTG                                                                                                                          >  SRR19973074.91329/1‑150 (MQ=60)
                            GGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTG                                                                                                                          >  SRR19973074.1020550/1‑150 (MQ=60)
                            GGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTG                                                                                                                          >  SRR19973074.91291/1‑150 (MQ=60)
                            GGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTG                                                                                                                          <  SRR19973074.1239485/150‑1 (MQ=60)
                              AGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGC                                                                                                                        >  SRR19973074.1752178/1‑150 (MQ=60)
                                CCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAG                                                                                                                      >  SRR19973074.2624993/1‑150 (MQ=60)
                                CCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAG                                                                                                                      <  SRR19973074.280139/150‑1 (MQ=60)
                                       CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAT                                                                                                               <  SRR19973074.1202785/150‑1 (MQ=60)
                                       CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAT                                                                                                               <  SRR19973074.1202932/150‑1 (MQ=60)
                                        TTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATT                                                                                                              <  SRR19973074.1401672/150‑1 (MQ=60)
                                          CTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAT                                                                                                            <  SRR19973074.3262688/150‑1 (MQ=60)
                                           TCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATG                                                                                                           >  SRR19973074.1172438/1‑150 (MQ=60)
                                           TCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATG                                                                                                           <  SRR19973074.2317129/150‑1 (MQ=60)
                                             CCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGC                                                                                                         >  SRR19973074.3191652/1‑150 (MQ=60)
                                             CCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGC                                                                                                         <  SRR19973074.1881632/150‑1 (MQ=60)
                                             CCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGC                                                                                                         >  SRR19973074.1096085/1‑150 (MQ=60)
                                              CTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGNCG                                                                                                        <  SRR19973074.2068799/150‑1 (MQ=60)
                                                  TGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCC                                                                                                    >  SRR19973074.1355570/1‑150 (MQ=60)
                                                  TGGCGAGTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCC                                                                                                    <  SRR19973074.2838608/150‑1 (MQ=60)
                                                   GGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCT                                                                                                   <  SRR19973074.1461247/150‑1 (MQ=60)
                                                     CGATTCCGTTCTTTGTGCTGGCGGGTGAAAACATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTT                                                                                                 >  SRR19973074.3868619/1‑150 (MQ=60)
                                                      GATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTC                                                                                                <  SRR19973074.2835667/150‑1 (MQ=60)
                                                       ATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCT                                                                                               <  SRR19973074.4161562/150‑1 (MQ=60)
                                                        TTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAGTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTG                                                                                              >  SRR19973074.1722585/1‑150 (MQ=60)
                                                         TCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGG                                                                                             <  SRR19973074.2627320/150‑1 (MQ=60)
                                                         TCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGG                                                                                             <  SRR19973074.2626086/150‑1 (MQ=60)
                                                         TCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGG                                                                                             <  SRR19973074.539206/150‑1 (MQ=60)
                                                             TTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCT                                                                                         <  SRR19973074.2631873/150‑1 (MQ=60)
                                                              TCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCANCTATGATTATGGCGAGCCTTTCTGGTTCTG                                                                                        <  SRR19973074.3859121/150‑1 (MQ=60)
                                                              TCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCANCTATGATTATGGCGAGCCTTTCTGGTTCTG                                                                                        <  SRR19973074.3859203/150‑1 (MQ=60)
                                                              TCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTG                                                                                        <  SRR19973074.3986181/150‑1 (MQ=60)
                                                               CTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGC                                                                                       <  SRR19973074.732708/150‑1 (MQ=60)
                                                                   GTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTT                                                                                   <  SRR19973074.3059684/150‑1 (MQ=60)
                                                                      CTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCG                                                                                <  SRR19973074.3174767/150‑1 (MQ=60)
                                                                       TGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGG                                                                               <  SRR19973074.1064250/150‑1 (MQ=60)
                                                                       TGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGG                                                                               >  SRR19973074.1618858/1‑150 (MQ=60)
                                                                            GGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACC                                                                          >  SRR19973074.3887680/1‑150 (MQ=60)
                                                                             GTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCG                                                                         <  SRR19973074.1890323/150‑1 (MQ=60)
                                                                              TGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGC                                                                        >  SRR19973074.311741/1‑150 (MQ=60)
                                                                               GAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCC                                                                       >  SRR19973074.3433768/1‑150 (MQ=60)
                                                                               GAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCC                                                                       >  SRR19973074.425578/1‑150 (MQ=60)
                                                                                 AATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGC                                                                     >  SRR19973074.984831/1‑150 (MQ=60)
                                                                                    CATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGT                                                                  >  SRR19973074.2763665/1‑150 (MQ=60)
                                                                                    CATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGT                                                                  >  SRR19973074.1687739/1‑150 (MQ=60)
                                                                                    CATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGT                                                                  >  SRR19973074.1687655/1‑150 (MQ=60)
                                                                                     ATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTT                                                                 <  SRR19973074.1952838/150‑1 (MQ=60)
                                                                                      TGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                                <  SRR19973074.3856526/150‑1 (MQ=60)
                                                                                      TGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                                <  SRR19973074.3716292/150‑1 (MQ=60)
                                                                                      TGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                                >  SRR19973074.1550265/1‑150 (MQ=60)
                                                                                      TGAATGCGGGCGGCTTGTCAAAACGCAGTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTG                                                                <  SRR19973074.3856552/150‑1 (MQ=60)
                                                                                       GAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGC                                                               <  SRR19973074.2864908/150‑1 (MQ=60)
                                                                                        ACTGCGGGCGGCTTGTCTAATCGCATTGTTGACCTGCCGATGAAACTGGTGGGTCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCC                                                              <  SRR19973074.3690362/150‑1 (MQ=60)
                                                                                        AATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCC                                                              <  SRR19973074.3959052/150‑1 (MQ=60)
                                                                                                CGGCTTGTCAAAACACATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACT                                                      <  SRR19973074.2324287/150‑1 (MQ=60)
                                                                                                 GGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTG                                                     <  SRR19973074.4166663/150‑1 (MQ=60)
                                                                                                 GGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTG                                                     >  SRR19973074.3381582/1‑150 (MQ=60)
                                                                                                 GGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTG                                                     >  SRR19973074.3381578/1‑150 (MQ=60)
                                                                                                    TTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTG                                                  <  SRR19973074.1746730/150‑1 (MQ=60)
                                                                                                     TGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGC                                                 <  SRR19973074.1386016/150‑1 (MQ=60)
                                                                                                         AAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGAT                                             >  SRR19973074.3430326/1‑150 (MQ=60)
                                                                                                          AAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATG                                            >  SRR19973074.1126451/1‑150 (MQ=60)
                                                                                                          AAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATG                                            >  SRR19973074.1126460/1‑150 (MQ=60)
                                                                                                          AAACGCATTGTTGACCTGCAGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATG                                            <  SRR19973074.1553551/150‑1 (MQ=60)
                                                                                                            ACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGAT                                          <  SRR19973074.1184919/150‑1 (MQ=60)
                                                                                                             CGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATG                                         >  SRR19973074.361973/1‑150 (MQ=60)
                                                                                                              GCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGC                                        <  SRR19973074.1709898/150‑1 (MQ=60)
                                                                                                              GCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGC                                        <  SRR19973074.1152671/150‑1 (MQ=60)
                                                                                                                ATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGC                                      >  SRR19973074.288428/1‑150 (MQ=60)
                                                                                                                ATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGC                                      >  SRR19973074.1311725/1‑150 (MQ=60)
                                                                                                                ATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGC                                      >  SRR19973074.233220/1‑150 (MQ=60)
                                                                                                                    TTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCG                                  <  SRR19973074.1078637/150‑1 (MQ=60)
                                                                                                                     TGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGC                                 >  SRR19973074.154339/1‑150 (MQ=60)
                                                                                                                      GACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCC                                >  SRR19973074.1747274/1‑150 (MQ=60)
                                                                                                                      GACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCC                                >  SRR19973074.2451147/1‑150 (MQ=60)
                                                                                                                      GACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCC                                >  SRR19973074.1747257/1‑150 (MQ=60)
                                                                                                                       ACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGTTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCA                               <  SRR19973074.1311901/150‑1 (MQ=60)
                                                                                                                       ACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCA                               <  SRR19973074.1311848/150‑1 (MQ=60)
                                                                                                                         CTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAAC                             >  SRR19973074.2450635/1‑150 (MQ=60)
                                                                                                                         CTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAAC                             >  SRR19973074.2450643/1‑150 (MQ=60)
                                                                                                                          TGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACT                            >  SRR19973074.3533650/1‑150 (MQ=60)
                                                                                                                          TGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACT                            <  SRR19973074.2233789/150‑1 (MQ=60)
                                                                                                                          TGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACT                            <  SRR19973074.2234025/150‑1 (MQ=60)
                                                                                                                             CGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGATGCCGCTCTACTGGTGCCGATGAAGCGCAGCGCCAACTATC                         >  SRR19973074.2938442/1‑150 (MQ=60)
                                                                                                                             CGATGAAACTGGTGGGGAATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATC                         >  SRR19973074.2938461/1‑150 (MQ=60)
                                                                                                                              GATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCC                        >  SRR19973074.2686239/1‑150 (MQ=60)
                                                                                                                              GATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCC                        >  SRR19973074.850529/1‑150 (MQ=60)
                                                                                                                               ATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCG                       >  SRR19973074.1022177/1‑150 (MQ=60)
                                                                                                                                TGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGG                      >  SRR19973074.2976068/1‑150 (MQ=60)
                                                                                                                                  AAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTC                    >  SRR19973074.543722/1‑150 (MQ=60)
                                                                                                                                     CTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAAC                 >  SRR19973074.876723/1‑150 (MQ=60)
                                                                                                                                       GGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCG               >  SRR19973074.1431569/1‑150 (MQ=60)
                                                                                                                                         TGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGG             <  SRR19973074.958346/150‑1 (MQ=60)
                                                                                                                                              CATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCG        <  SRR19973074.454309/150‑1 (MQ=60)
                                                                                                                                               ATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGG       <  SRR19973074.2217150/150‑1 (MQ=60)
                                                                                                                                               ATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGG       >  SRR19973074.3270158/1‑150 (MQ=60)
                                                                                                                                               ATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGG       >  SRR19973074.3270135/1‑150 (MQ=60)
                                                                                                                                                TAAAACAGGCGGGCTGGGCTACGTCGGCGTNCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGG      >  SRR19973074.3866213/1‑150 (MQ=60)
                                                                                                                                                TAAAACAGGCGGGCTGGGCTACGTCGGCGTNCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGG      >  SRR19973074.3866215/1‑150 (MQ=60)
                                                                                                                                                 AAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGG     >  SRR19973074.1392294/1‑150 (MQ=60)
                                                                                                                                                   AACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCT   <  SRR19973074.2190542/150‑1 (MQ=60)
                                                                                                                                                    ACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  >  SRR19973074.2572109/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  >  CP009273/3738283‑3738580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑