Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,789,381 C→A 90.1% L15I (CTC→ATC)  waaC → ADP‑heptose:LPS heptosyl transferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,789,3810CA90.1% 444.2 / 44.5 172L15I (CTC→ATC) waaCADP‑heptose:LPS heptosyl transferase I
Reads supporting (aligned to +/- strand):  ref base C (8/9);  new base A (67/88);  total (75/97)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 172 total aligned reads displayed.

GGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGG  >  CP009273/3789237‑3789524
                                                                                                                                                |                                                                                                                                               
ggTGACGCTGCGGAGGGTTATCACCAGAGCTTACTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATcc                                                                                                                                              <  2:738627/148‑1 (MQ=255)
 gTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCa                                                                                                                                             <  2:549361/148‑1 (MQ=255)
  tGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCAt                                                                                                                                            <  1:2987881/148‑1 (MQ=255)
  tGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCAt                                                                                                                                            <  2:2152024/148‑1 (MQ=255)
     cGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACg                                                                                                                                         <  1:2157805/148‑1 (MQ=255)
      gCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGt                                                                                                                                        <  1:679330/148‑1 (MQ=255)
      gCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGt                                                                                                                                        <  2:1003631/148‑1 (MQ=255)
      gCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGt                                                                                                                                        <  1:3215834/148‑1 (MQ=255)
       cTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGtt                                                                                                                                       >  2:1981556/1‑148 (MQ=255)
         gCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGc                                                                                                                                     <  1:1746376/148‑1 (MQ=255)
                   aTCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcactcac                                                                                                                           >  2:3009677/1‑148 (MQ=255)
                    tCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcactcact                                                                                                                          >  2:1979267/1‑148 (MQ=255)
                     cACCAGAGCTTACTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTg                                                                                                                         <  1:563473/148‑1 (MQ=255)
                        cAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATg                                                                                                                      <  2:2967918/148‑1 (MQ=255)
                        cAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATg                                                                                                                      >  1:3031920/1‑148 (MQ=255)
                        cAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATg                                                                                                                      >  1:3031891/1‑148 (MQ=255)
                         agagCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGc                                                                                                                     <  2:2646697/148‑1 (MQ=255)
                            gCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCca                                                                                                                  >  2:1346953/1‑148 (MQ=255)
                              ttAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagc                                                                                                                <  2:2668544/148‑1 (MQ=255)
                              ttAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagc                                                                                                                <  2:2668455/148‑1 (MQ=255)
                              ttAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagc                                                                                                                >  2:111972/1‑148 (MQ=255)
                               tAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagca                                                                                                               >  2:2255265/1‑148 (MQ=255)
                               tAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagca                                                                                                               >  2:1429998/1‑148 (MQ=255)
                               aaaTCGACAGTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagca                                                                                                               <  2:1011710/147‑1 (MQ=255)
                                   cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                           >  2:788754/1‑148 (MQ=255)
                                   cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                           <  2:3120630/148‑1 (MQ=255)
                                    gACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCaa                                                                                                          <  1:2463194/148‑1 (MQ=255)
                                      cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATc                                                                                                        >  1:825044/1‑148 (MQ=255)
                                      cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATc                                                                                                        >  1:2277882/1‑148 (MQ=255)
                                      cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATc                                                                                                        >  1:2085959/1‑148 (MQ=255)
                                       aTTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATcc                                                                                                       <  1:1569091/148‑1 (MQ=255)
                                         tACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCa                                                                                                     <  2:1779486/148‑1 (MQ=255)
                                         tACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCa                                                                                                     <  2:310112/148‑1 (MQ=255)
                                            tCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAggg                                                                                                  <  2:1022448/148‑1 (MQ=255)
                                               ccAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGAtt                                                                                               >  2:743460/1‑148 (MQ=255)
                                                cAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCACTCCCAGGGATTa                                                                                              >  1:2550372/1‑148 (MQ=255)
                                                cAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTa                                                                                              >  1:896127/1‑148 (MQ=255)
                                                cAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTa                                                                                              <  1:1379030/148‑1 (MQ=255)
                                                cAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTa                                                                                              <  2:2742767/148‑1 (MQ=255)
                                                 aGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTaa                                                                                             <  1:2931327/148‑1 (MQ=255)
                                                 aGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTaa                                                                                             <  1:1278678/148‑1 (MQ=255)
                                                  gcgcGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCGTGACGGATGGGGGTTTTGATCTTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAg                                                                                            <  2:3075614/148‑1 (MQ=255)
                                                  gcgcGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAg                                                                                            <  1:2404708/148‑1 (MQ=255)
                                                   cgcgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGt                                                                                           >  1:1648466/1‑148 (MQ=255)
                                                   cgcgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGt                                                                                           <  2:291602/148‑1 (MQ=255)
                                                       tACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGa                                                                                       <  1:448723/148‑1 (MQ=255)
                                                        aCTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGAc                                                                                      >  2:1107193/1‑148 (MQ=255)
                                                        aCTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGAc                                                                                      <  2:3130784/148‑1 (MQ=255)
                                                         cTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACt                                                                                     <  2:1204987/148‑1 (MQ=255)
                                                          tGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTg                                                                                    <  2:346188/148‑1 (MQ=255)
                                                          tGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTg                                                                                    <  2:2105964/148‑1 (MQ=255)
                                                               gaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtgg                                                                               >  1:1219830/1‑148 (MQ=255)
                                                               gaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtgg                                                                               >  2:2588032/1‑148 (MQ=255)
                                                                aaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtggt                                                                              <  2:1131263/148‑1 (MQ=255)
                                                                    cAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaa                                                                          >  1:3278402/1‑148 (MQ=255)
                                                                    cAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaa                                                                          <  1:2762609/148‑1 (MQ=255)
                                                                      aCGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaaga                                                                        <  1:1170826/148‑1 (MQ=255)
                                                                      aCGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaaga                                                                        >  1:1489014/1‑148 (MQ=255)
                                                                       cgcgCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaagaa                                                                       >  1:3133040/1‑148 (MQ=255)
                                                                          gcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAggg                                                                    <  1:1282796/148‑1 (MQ=255)
                                                                          gcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAggg                                                                    <  1:3084581/148‑1 (MQ=255)
                                                                           cTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGt                                                                   <  1:2100323/148‑1 (MQ=255)
                                                                           cTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGt                                                                   >  2:2764762/1‑148 (MQ=255)
                                                                            tATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGtt                                                                  <  1:403847/148‑1 (MQ=255)
                                                                              ttGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCg                                                                >  2:1356667/1‑148 (MQ=255)
                                                                                 ttACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGcac                                                             <  1:546956/148‑1 (MQ=255)
                                                                                   aCAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAg                                                           <  1:727219/148‑1 (MQ=255)
                                                                                    cAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGa                                                          <  1:1403583/148‑1 (MQ=255)
                                                                                    cAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGa                                                          <  1:2752061/148‑1 (MQ=255)
                                                                                      agagGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAtt                                                        >  2:2454747/1‑148 (MQ=255)
                                                                                      agagGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAtt                                                        >  2:2454169/1‑148 (MQ=255)
                                                                                         ggAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttcct                                                     <  2:607443/148‑1 (MQ=255)
                                                                                           aaGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttc                                                   <  2:36990/148‑1 (MQ=255)
                                                                                           aaGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttc                                                   <  1:433487/148‑1 (MQ=255)
                                                                                                  aCGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACGGGGTGGTGGCAGAAGGGTTCGCCCAGATTCCTGCCTGGCAc                                            >  2:1978297/1‑148 (MQ=255)
                                                                                                   cGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACg                                           <  2:2277882/148‑1 (MQ=255)
                                                                                                       tGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGc                                       <  1:2628940/148‑1 (MQ=255)
                                                                                                       tGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGc                                       <  1:2628923/148‑1 (MQ=255)
                                                                                                         cGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCg                                     >  2:3022467/1‑148 (MQ=255)
                                                                                                         cGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCg                                     <  1:2561967/148‑1 (MQ=255)
                                                                                                          gggTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGt                                    <  2:2916829/148‑1 (MQ=255)
                                                                                                              tttGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAg                                >  2:1788017/1‑148 (MQ=255)
                                                                                                              tttGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAg                                >  2:1788097/1‑148 (MQ=255)
                                                                                                                 gATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGa                             <  1:1106970/148‑1 (MQ=255)
                                                                                                                      ttAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAt                        <  1:2313652/148‑1 (MQ=255)
                                                                                                                        aaaaCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTc                      <  1:1346953/148‑1 (MQ=255)
                                                                                                                          aaCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCt                    >  1:2735536/1‑148 (MQ=255)
                                                                                                                           aCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtg                   >  1:549355/1‑148 (MQ=255)
                                                                                                                           aCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtg                   >  2:1530660/1‑148 (MQ=255)
                                                                                                                            cATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtgt                  <  1:1532735/148‑1 (MQ=255)
                                                                                                                                gtcgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCa              <  2:825192/148‑1 (MQ=255)
                                                                                                                                  cgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGTGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAt            >  1:2490423/1‑148 (MQ=255)
                                                                                                                                  cgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAACAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAt            >  1:2725197/1‑148 (MQ=255)
                                                                                                                                    aTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc          >  2:3306290/1‑148 (MQ=255)
                                                                                                                                    aTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc          >  2:1370949/1‑148 (MQ=255)
                                                                                                                                     tGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACg         <  1:65387/148‑1 (MQ=255)
                                                                                                                                     tGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACg         >  1:1874999/1‑148 (MQ=255)
                                                                                                                                       ggCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt                       <  2:256821/132‑1 (MQ=255)
                                                                                                                                            tGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTgg  >  1:2513637/1‑148 (MQ=255)
                                                                                                                                            tGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTgg  >  2:1664854/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGG  >  CP009273/3789237‑3789524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGC  >  CP009273/3789235‑3789525
                                                                                                                                                  |                                                                                                                                                
AAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCC                                                                                                                                               >  SRR19973070.2129133/1‑150 (MQ=60)
    TGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATAC                                                                                                                                           <  SRR19973070.3007029/150‑1 (MQ=60)
       CGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTT                                                                                                                                        <  SRR19973070.2711282/150‑1 (MQ=60)
       CGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTT                                                                                                                                        <  SRR19973070.2172151/150‑1 (MQ=60)
        GCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATCCGTTG                                                                                                                                       <  SRR19973070.683255/150‑1 (MQ=60)
        GCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTG                                                                                                                                       <  SRR19973070.684172/150‑1 (MQ=60)
        GCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTG                                                                                                                                       <  SRR19973070.3236628/150‑1 (MQ=60)
        GCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTG                                                                                                                                       <  SRR19973070.3236625/150‑1 (MQ=60)
           GCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCC                                                                                                                                    <  SRR19973070.1758485/150‑1 (MQ=60)
             GGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGC                                                                                                                                  >  SRR19973070.2571178/1‑150 (MQ=60)
             GGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGC                                                                                                                                  >  SRR19973070.54853/1‑150 (MQ=60)
                       CACCAGAGCTTACTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGAT                                                                                                                        <  SRR19973070.567231/150‑1 (MQ=60)
                        ACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATG                                                                                                                       >  SRR19973070.3051193/1‑150 (MQ=60)
                        ACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATG                                                                                                                       >  SRR19973070.3051212/1‑150 (MQ=60)
                        ACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATG                                                                                                                       >  SRR19973070.3051182/1‑150 (MQ=60)
                          CAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCC                                                                                                                     <  SRR19973070.1544708/150‑1 (MQ=60)
                            GAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCA                                                                                                                   <  SRR19973070.2967703/150‑1 (MQ=60)
                                      GACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATC                                                                                                         <  SRR19973070.2479274/150‑1 (MQ=60)
                                      GACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATC                                                                                                         >  SRR19973070.2293013/1‑150 (MQ=60)
                                      GACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATC                                                                                                         >  SRR19973070.830657/1‑150 (MQ=60)
                                      GACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATC                                                                                                         >  SRR19973070.830806/1‑150 (MQ=60)
                                      GACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATC                                                                                                         >  SRR19973070.2099990/1‑150 (MQ=60)
                                       ACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCC                                                                                                        >  SRR19973070.1408976/1‑150 (MQ=60)
                                         ATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCA                                                                                                      <  SRR19973070.1580121/150‑1 (MQ=60)
                                           TACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGG                                                                                                    >  SRR19973070.1377501/1‑150 (MQ=60)
                                               CCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAGAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCAAGGGATT                                                                                                <  SRR19973070.623240/150‑1 (MQ=60)
                                               CCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATT                                                                                                <  SRR19973070.907748/150‑1 (MQ=60)
                                                CCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCACTCCCAGGGATTA                                                                                               >  SRR19973070.2567053/1‑150 (MQ=60)
                                                CCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTA                                                                                               >  SRR19973070.902470/1‑150 (MQ=60)
                                                CCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTA                                                                                               <  SRR19973070.27620/150‑1 (MQ=60)
                                                  CAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAG                                                                                             <  SRR19973070.1388383/150‑1 (MQ=60)
                                                   AGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGT                                                                                            <  SRR19973070.2950238/150‑1 (MQ=60)
                                                   AGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGT                                                                                            >  SRR19973070.1659847/1‑150 (MQ=60)
                                                   AGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGT                                                                                            <  SRR19973070.1287608/150‑1 (MQ=60)
                                                    GCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTT                                                                                           <  SRR19973070.2420569/150‑1 (MQ=60)
                                                    GCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTT                                                                                           <  SRR19973070.1215081/150‑1 (MQ=60)
                                                     CGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTT                                                                                          >  SRR19973070.124266/1‑150 (MQ=60)
                                                         TACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACT                                                                                      <  SRR19973070.451961/150‑1 (MQ=60)
                                                         TACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACT                                                                                      <  SRR19973070.451847/150‑1 (MQ=60)
                                                               AAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGG                                                                                >  SRR19973070.1228379/1‑150 (MQ=60)
                                                                 GAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTG                                                                              <  SRR19973070.1696296/150‑1 (MQ=60)
                                                                    CTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAA                                                                           >  SRR19973070.3299469/1‑150 (MQ=60)
                                                                    CTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAA                                                                           <  SRR19973070.183297/150‑1 (MQ=60)
                                                                      CAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGA                                                                         <  SRR19973070.2095158/150‑1 (MQ=60)
                                                                      CAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGA                                                                         >  SRR19973070.1499435/1‑150 (MQ=60)
                                                                      CAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGA                                                                         <  SRR19973070.2780313/150‑1 (MQ=60)
                                                                      CAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGACCAGCAGGCAATCCCAGGGATTAAGTTTGACTGTGTGGTGGAAGA                                                                         >  SRR19973070.1499765/1‑150 (MQ=60)
                                                                       AACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAA                                                                        >  SRR19973070.3153278/1‑150 (MQ=60)
                                                                        ACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAG                                                                       <  SRR19973070.1179023/150‑1 (MQ=60)
                                                                          GCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGG                                                                     <  SRR19973070.2433866/150‑1 (MQ=60)
                                                                            GCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTT                                                                   <  SRR19973070.1291754/150‑1 (MQ=60)
                                                                            GCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTT                                                                   <  SRR19973070.3104420/150‑1 (MQ=60)
                                                                             CTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTC                                                                  <  SRR19973070.2114417/150‑1 (MQ=60)
                                                                              TATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCG                                                                 <  SRR19973070.406812/150‑1 (MQ=60)
                                                                              TATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCG                                                                 <  SRR19973070.406803/150‑1 (MQ=60)
                                                                                   TTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAG                                                            <  SRR19973070.550604/150‑1 (MQ=60)
                                                                                     ACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAT                                                          <  SRR19973070.732378/150‑1 (MQ=60)
                                                                                      CAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATT                                                         <  SRR19973070.2769739/150‑1 (MQ=60)
                                                                                      CAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATT                                                         <  SRR19973070.734738/150‑1 (MQ=60)
                                                                                      CAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATT                                                         <  SRR19973070.1413087/150‑1 (MQ=60)
                                                                                      CAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATT                                                         >  SRR19973070.219376/1‑150 (MQ=60)
                                                                                      CAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATT                                                         <  SRR19973070.3070406/150‑1 (MQ=60)
                                                                                          AGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTT                                                     <  SRR19973070.3286344/150‑1 (MQ=60)
                                                                                             AAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCT                                                  <  SRR19973070.436638/150‑1 (MQ=60)
                                                                                                  TGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCAC                                             >  SRR19973070.2777543/1‑150 (MQ=60)
                                                                                                         TGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCG                                      <  SRR19973070.2646087/150‑1 (MQ=60)
                                                                                                         TGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCG                                      <  SRR19973070.2646070/150‑1 (MQ=60)
                                                                                                           CGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTT                                    <  SRR19973070.2550494/150‑1 (MQ=60)
                                                                                                           CGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTT                                    <  SRR19973070.2578711/150‑1 (MQ=60)
                                                                                                                   GATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGT                            <  SRR19973070.1114554/150‑1 (MQ=60)
                                                                                                                        TTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTC                       <  SRR19973070.2329027/150‑1 (MQ=60)
                                                                                                                          AAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCT                     <  SRR19973070.1356195/150‑1 (MQ=60)
                                                                                                                          AAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCT                     >  SRR19973070.2753156/1‑150 (MQ=60)
                                                                                                                           AAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTG                    >  SRR19973070.553008/1‑150 (MQ=60)
                                                                                                                              CATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGG                 <  SRR19973070.1543497/150‑1 (MQ=60)
                                                                                                                              CATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTACCGTTGAGCGAGTTATTCCTGTGG                 >  SRR19973070.287088/1‑150 (MQ=60)
                                                                                                                                  GTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGTGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAT             >  SRR19973070.2506590/1‑150 (MQ=60)
                                                                                                                                  GTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAT             <  SRR19973070.553641/150‑1 (MQ=60)
                                                                                                                                  GTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAT             >  SRR19973070.1340405/1‑150 (MQ=60)
                                                                                                                                  GTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAACAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAT             >  SRR19973070.2742777/1‑150 (MQ=60)
                                                                                                                                    CGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAC           >  SRR19973070.249334/1‑150 (MQ=60)
                                                                                                                                     GATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACG          >  SRR19973070.1887774/1‑150 (MQ=60)
                                                                                                                                       TGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTC        <  SRR19973070.66118/150‑1 (MQ=60)
                                                                                                                                       TGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCagatcggaagagca        >  SRR19973070.258552/1‑136 (MQ=60)
                                                                                                                                            GATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGG   >  SRR19973070.2530012/1‑150 (MQ=60)
                                                                                                                                             ATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGC  <  SRR19973070.2374940/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                
AAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGC  >  CP009273/3789235‑3789525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑