Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,789,381 C→A 100% L15I (CTC→ATC)  waaC → ADP‑heptose:LPS heptosyl transferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,789,3810CA97.4% 633.0 / 6.1 204L15I (CTC→ATC) waaCADP‑heptose:LPS heptosyl transferase I
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (108/90);  total (113/91)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

AGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGC  >  CP009273/3789236‑3789525
                                                                                                                                                 |                                                                                                                                                
aGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtatc                                                                                                                                                <  1:2112081/148‑1 (MQ=255)
   tGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCAt                                                                                                                                             <  2:2067616/148‑1 (MQ=255)
   tGACGCTGCGGAGGGTTATCACCAGAGATTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCAt                                                                                                                                             <  1:3215957/148‑1 (MQ=255)
     aCGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATAc                                                                                                                                           >  1:2893805/1‑148 (MQ=255)
         tGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTg                                                                                                                                       <  1:3446428/148‑1 (MQ=255)
          gCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTAGATGGGCGATGTTATCCATACGTTGc                                                                                                                                      <  1:2004191/148‑1 (MQ=255)
             gAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCg                                                                                                                                   >  2:2833333/1‑148 (MQ=255)
               gggTTATCACCAGAGCTTACTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGca                                                                                                                                 <  2:1697158/148‑1 (MQ=255)
                  ttATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcactc                                                                                                                              >  1:2328218/1‑148 (MQ=255)
                      cACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTg                                                                                                                          >  2:1655617/1‑148 (MQ=255)
                      cACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTg                                                                                                                          >  1:1232803/1‑148 (MQ=255)
                      cACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTg                                                                                                                          <  2:2605617/148‑1 (MQ=255)
                       aCCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGGACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGa                                                                                                                         <  1:1347219/148‑1 (MQ=255)
                           gagCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGcc                                                                                                                     <  2:3183427/148‑1 (MQ=255)
                            agCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGccc                                                                                                                    >  1:1150747/1‑148 (MQ=255)
                              cTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcag                                                                                                                  >  1:1228758/1‑148 (MQ=255)
                              cTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcag                                                                                                                  >  2:1398697/1‑148 (MQ=255)
                                tAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagca                                                                                                                >  1:374875/1‑148 (MQ=255)
                                  ctCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAgg                                                                                                              <  1:618490/147‑1 (MQ=255)
                                  aTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAgg                                                                                                              <  1:3100228/148‑1 (MQ=255)
                                  aTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAgg                                                                                                              >  2:1685929/1‑148 (MQ=255)
                                   tCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGc                                                                                                             >  2:3293564/1‑148 (MQ=255)
                                    cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                            >  1:2530336/1‑148 (MQ=255)
                                    cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                            <  1:1461832/148‑1 (MQ=255)
                                       cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATc                                                                                                         >  2:493148/1‑148 (MQ=255)
                                         ttACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATccc                                                                                                       >  1:411843/1‑148 (MQ=255)
                                           aCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAg                                                                                                     <  1:2875429/148‑1 (MQ=255)
                                           aCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAg                                                                                                     <  2:597870/148‑1 (MQ=255)
                                             tCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAggg                                                                                                   <  2:2463312/148‑1 (MQ=255)
                                             tCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAggg                                                                                                   <  1:1031043/148‑1 (MQ=255)
                                             tCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAggg                                                                                                   <  2:311943/148‑1 (MQ=255)
                                             tCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAggg                                                                                                   >  2:1069991/1‑148 (MQ=255)
                                              ccccAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGa                                                                                                  <  2:2726734/148‑1 (MQ=255)
                                               cccAGCGCGTACTGGAAGTACTCATCGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCTGGGAt                                                                                                 <  2:504946/148‑1 (MQ=255)
                                                 cAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTa                                                                                               <  1:1614357/148‑1 (MQ=255)
                                                  aGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTaa                                                                                              >  1:3142928/1‑148 (MQ=255)
                                                   gcgcGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAg                                                                                             >  1:2962857/1‑148 (MQ=255)
                                                    cgcgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGt                                                                                            >  2:2618386/1‑148 (MQ=255)
                                                    cgcgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGAGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGt                                                                                            <  1:2366088/148‑1 (MQ=255)
                                                        tACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGa                                                                                        >  1:2151649/1‑148 (MQ=255)
                                                        tACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGa                                                                                        <  2:1008313/148‑1 (MQ=255)
                                                        tACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGa                                                                                        <  2:856403/148‑1 (MQ=255)
                                                         aCTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGAc                                                                                       <  2:469183/148‑1 (MQ=255)
                                                          cttgaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACt                                                                                      <  2:2185187/145‑1 (MQ=255)
                                                          cTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGTGGGTGTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACt                                                                                      >  2:393300/1‑148 (MQ=255)
                                                             gaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTggg                                                                                   <  1:846446/148‑1 (MQ=255)
                                                             gaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGGTCGCCATACGTTGCCCGAACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTggg                                                                                   <  1:3447523/148‑1 (MQ=255)
                                                              aagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggt                                                                                  >  2:1657557/1‑148 (MQ=255)
                                                               agaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtg                                                                                 <  1:2514915/148‑1 (MQ=255)
                                                                gaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtgg                                                                                <  1:897535/148‑1 (MQ=255)
                                                                gaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtgg                                                                                <  1:474708/148‑1 (MQ=255)
                                                                gaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtgg                                                                                >  1:1352661/1‑148 (MQ=255)
                                                                gaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtgg                                                                                <  1:1858207/148‑1 (MQ=255)
                                                                 aaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtggt                                                                               >  2:1765446/1‑148 (MQ=255)
                                                                  aCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGgtggtg                                                                              <  1:2630581/148‑1 (MQ=255)
                                                                   cTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGtggtgg                                                                             >  1:717833/1‑148 (MQ=255)
                                                                     cAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaa                                                                           >  1:141806/1‑148 (MQ=255)
                                                                          cgcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAgg                                                                      <  2:1817823/148‑1 (MQ=255)
                                                                           gcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAggg                                                                     <  2:239707/148‑1 (MQ=255)
                                                                            cTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGt                                                                    >  2:2324847/1‑148 (MQ=255)
                                                                             tATTGTTACAAGAGGAAGCCTGACGGATGCGTGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATACATACGTTGCCCGCACTCACTGATTCCCAGCAGGCAATCACAGGGATTAAGTTTGACTGGGTGGTGGAAGAATGGtt                                                                   >  1:951022/1‑148 (MQ=255)
                                                                                 gTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGca                                                               <  1:2675211/148‑1 (MQ=255)
                                                                                 gTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGca                                                               <  2:2687478/148‑1 (MQ=255)
                                                                                   tACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGcaca                                                             >  2:1634080/1‑148 (MQ=255)
                                                                                      aaGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAt                                                          >  2:878612/1‑148 (MQ=255)
                                                                                       agagGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAtt                                                         >  2:1963433/1‑148 (MQ=255)
                                                                                         agGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttcc                                                       >  2:923439/1‑148 (MQ=255)
                                                                                           gAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttcctt                                                     >  1:701075/1‑148 (MQ=255)
                                                                                           gAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttcctt                                                     >  1:2606644/1‑148 (MQ=255)
                                                                                              gCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATtccttcct                                                  >  1:2523513/1‑148 (MQ=255)
                                                                                                    cGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACg                                            <  2:3068681/148‑1 (MQ=255)
                                                                                                       aTGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTg                                         <  1:1988897/148‑1 (MQ=255)
                                                                                                       aTGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTg                                         >  2:1513182/1‑148 (MQ=255)
                                                                                                              ttttGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGa                                  >  1:1880702/1‑148 (MQ=255)
                                                                                                                ttGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGc                                <  2:2953721/148‑1 (MQ=255)
                                                                                                                    tCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                            >  1:3503170/1‑148 (MQ=255)
                                                                                                                    tCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                            >  2:1470492/1‑148 (MQ=255)
                                                                                                                    tCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                            >  1:1200610/1‑148 (MQ=255)
                                                                                                                     cGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGtt                           <  1:3293564/148‑1 (MQ=255)
                                                                                                                      gTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTa                          <  2:2781125/148‑1 (MQ=255)
                                                                                                                      gTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTa                          <  2:2893805/148‑1 (MQ=255)
                                                                                                                       ttAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAt                         >  2:587554/1‑148 (MQ=255)
                                                                                                                        tAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt                        >  1:466364/1‑148 (MQ=255)
                                                                                                                         aaaaCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTc                       <  2:3134685/148‑1 (MQ=255)
                                                                                                                          aaaCATCGTCGATGGGCGATGTTATCCAAACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTcc                      >  2:968327/1‑148 (MQ=255)
                                                                                                                           aaCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCt                     >  2:3459334/1‑148 (MQ=255)
                                                                                                                            aCATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtg                    >  2:1989331/1‑148 (MQ=255)
                                                                                                                             cATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtgt                   >  2:193800/1‑148 (MQ=255)
                                                                                                                               tcgtcgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTgg                 <  2:2382498/148‑1 (MQ=255)
                                                                                                                                cgtcgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGc                >  2:2918692/1‑148 (MQ=255)
                                                                                                                                 gtcgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCa               <  2:919110/148‑1 (MQ=255)
                                                                                                                                       gggCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGt         >  2:689122/1‑148 (MQ=255)
                                                                                                                                       gggCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGt         >  1:2844183/1‑148 (MQ=255)
                                                                                                                                        ggCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTc        >  2:1039248/1‑148 (MQ=255)
                                                                                                                                            aTGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTg    >  2:2158731/1‑148 (MQ=255)
                                                                                                                                             tGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTgg   >  1:1525046/1‑148 (MQ=255)
                                                                                                                                              gTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTgtc  >  2:2244929/1‑146 (MQ=255)
                                                                                                                                              gTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGc  >  2:2523837/1‑148 (MQ=255)
                                                                                                                                              gTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGc  >  2:1376224/1‑148 (MQ=255)
                                                                                                                                              gTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGc  >  2:1109276/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGC  >  CP009273/3789236‑3789525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAA  >  CP009273/3789232‑3789529
                                                                                                                                                     |                                                                                                                                                    
GTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTA                                                                                                                                                      <  SRR19973065.1984855/150‑1 (MQ=60)
GTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTA                                                                                                                                                      <  SRR19973065.1987001/150‑1 (MQ=60)
   AAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCC                                                                                                                                                   <  SRR19973065.3202891/150‑1 (MQ=60)
   AAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCC                                                                                                                                                   <  SRR19973065.1329581/150‑1 (MQ=60)
    TGGTGACGCTGCGGTGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCA                                                                                                                                                  <  SRR19973065.2125685/150‑1 (MQ=60)
    AGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCA                                                                                                                                                  <  SRR19973065.2125673/150‑1 (MQ=60)
       TGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATAC                                                                                                                                               >  SRR19973065.2911479/1‑150 (MQ=60)
       TGACGCTGCGGAGGGTTATCACCAGAGATTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATAC                                                                                                                                               <  SRR19973065.3235298/150‑1 (MQ=60)
             TGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCC                                                                                                                                         <  SRR19973065.1421817/150‑1 (MQ=60)
             TGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCC                                                                                                                                         >  SRR19973065.2022158/1‑150 (MQ=60)
             TGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCC                                                                                                                                         <  SRR19973065.3467437/150‑1 (MQ=60)
              GCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTAGATGGGCGATGTTATCCATACGTTGCCC                                                                                                                                        <  SRR19973065.2017145/150‑1 (MQ=60)
              GCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTAGATGGGCGATGTTATCCATACGTTGCCC                                                                                                                                        <  SRR19973065.2017147/150‑1 (MQ=60)
                    GGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTC                                                                                                                                  >  SRR19973065.2342782/1‑150 (MQ=60)
                    GGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTC                                                                                                                                  >  SRR19973065.2982579/1‑150 (MQ=60)
                        ATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTG                                                                                                                              >  SRR19973065.1240876/1‑150 (MQ=60)
                        ATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTG                                                                                                                              >  SRR19973065.1240830/1‑150 (MQ=60)
                        ATCACAAGAGATTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGTGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTG                                                                                                                              <  SRR19973065.29171/150‑1 (MQ=60)
                           ACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGGACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATG                                                                                                                           <  SRR19973065.1356128/150‑1 (MQ=60)
                              AGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCC                                                                                                                        >  SRR19973065.2919880/1‑150 (MQ=60)
                              AGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCC                                                                                                                        >  SRR19973065.2798381/1‑150 (MQ=60)
                              AGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCC                                                                                                                        >  SRR19973065.1158159/1‑150 (MQ=60)
                               GAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCTCTGATGCCCA                                                                                                                       <  SRR19973065.1429402/150‑1 (MQ=60)
                                AGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAG                                                                                                                      >  SRR19973065.1236783/1‑150 (MQ=60)
                                  CTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCA                                                                                                                    >  SRR19973065.377493/1‑150 (MQ=60)
                                     AATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGC                                                                                                                 >  SRR19973065.26037/1‑150 (MQ=60)
                                      CTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCA                                                                                                                <  SRR19973065.622465/150‑1 (MQ=60)
                                      ATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCA                                                                                                                >  SRR19973065.2546219/1‑150 (MQ=60)
                                      ATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCA                                                                                                                <  SRR19973065.3119154/150‑1 (MQ=60)
                                        CGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAAT                                                                                                              >  SRR19973065.1522993/1‑150 (MQ=60)
                                        CGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAAT                                                                                                              <  SRR19973065.1471226/150‑1 (MQ=60)
                                           CATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCC                                                                                                           >  SRR19973065.414724/1‑150 (MQ=60)
                                           CATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCC                                                                                                           >  SRR19973065.925127/1‑150 (MQ=60)
                                              TACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGG                                                                                                        <  SRR19973065.2683336/150‑1 (MQ=60)
                                               ACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGG                                                                                                       <  SRR19973065.2893044/150‑1 (MQ=60)
                                                 TCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGAT                                                                                                     <  SRR19973065.1037873/150‑1 (MQ=60)
                                                  CCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATT                                                                                                    <  SRR19973065.3467873/150‑1 (MQ=60)
                                                  CCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATT                                                                                                    <  SRR19973065.3468169/150‑1 (MQ=60)
                                                    CCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAA                                                                                                  >  SRR19973065.3162024/1‑150 (MQ=60)
                                                     CAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAG                                                                                                 >  SRR19973065.2980958/1‑150 (MQ=60)
                                                     CAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAG                                                                                                 <  SRR19973065.1077504/150‑1 (MQ=60)
                                                     CAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAG                                                                                                 <  SRR19973065.1625188/150‑1 (MQ=60)
                                                        CGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTT                                                                                              >  SRR19973065.3274627/1‑150 (MQ=60)
                                                        CGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTT                                                                                              >  SRR19973065.861512/1‑150 (MQ=60)
                                                        CGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTT                                                                                              <  SRR19973065.2380759/150‑1 (MQ=60)
                                                        CGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGAGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTT                                                                                              <  SRR19973065.2380833/150‑1 (MQ=60)
                                                          CGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGA                                                                                            >  SRR19973065.2165482/1‑150 (MQ=60)
                                                                 GAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTG                                                                                     <  SRR19973065.851934/150‑1 (MQ=60)
                                                                 GAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTG                                                                                     <  SRR19973065.851912/150‑1 (MQ=60)
                                                                 GAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGGTCGCCATACGTTGCCCGAACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTG                                                                                     <  SRR19973065.3468538/150‑1 (MQ=60)
                                                                  AAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGG                                                                                    >  SRR19973065.1361615/1‑150 (MQ=60)
                                                                   AGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGT                                                                                   <  SRR19973065.2530715/150‑1 (MQ=60)
                                                                    GAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTG                                                                                  <  SRR19973065.477950/150‑1 (MQ=60)
                                                                    GAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTG                                                                                  <  SRR19973065.1870687/150‑1 (MQ=60)
                                                                    GAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTG                                                                                  <  SRR19973065.1870431/150‑1 (MQ=60)
                                                                    GAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTG                                                                                  <  SRR19973065.903273/150‑1 (MQ=60)
                                                                     AACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGG                                                                                 >  SRR19973065.722637/1‑150 (MQ=60)
                                                                      ACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGA                                                                                <  SRR19973065.2646845/150‑1 (MQ=60)
                                                                       CTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAA                                                                               >  SRR19973065.143013/1‑150 (MQ=60)
                                                                        TCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAG                                                                              <  SRR19973065.1361610/150‑1 (MQ=60)
                                                                              CGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGT                                                                        >  SRR19973065.2491730/1‑150 (MQ=60)
                                                                               GCTATTGTTACAAGAGGAAGCCTGACGGATGCGTGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATACATACGTTGCCCGCACTCACTGATTCCCAGCAGGCAATCACAGGGATTAAGTTTGACTGGGTGGTGGAAGAATGGTT                                                                       >  SRR19973065.957307/1‑150 (MQ=60)
                                                                                CTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTC                                                                      >  SRR19973065.3155675/1‑150 (MQ=60)
                                                                                     GTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACA                                                                 <  SRR19973065.2691790/150‑1 (MQ=60)
                                                                                      TTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAG                                                                <  SRR19973065.3207991/150‑1 (MQ=60)
                                                                                      TTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAG                                                                <  SRR19973065.1326829/150‑1 (MQ=60)
                                                                                         CAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATT                                                             <  SRR19973065.1667591/150‑1 (MQ=60)
                                                                                             AGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTT                                                         >  SRR19973065.2622820/1‑150 (MQ=60)
                                                                                             AGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTT                                                         >  SRR19973065.705739/1‑150 (MQ=60)
                                                                                             AGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTT                                                         >  SRR19973065.705897/1‑150 (MQ=60)
                                                                                                AAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCT                                                      >  SRR19973065.2539468/1‑150 (MQ=60)
                                                                                                AAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCT                                                      >  SRR19973065.2539355/1‑150 (MQ=60)
                                                                                                AAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCT                                                      >  SRR19973065.2537389/1‑150 (MQ=60)
                                                                                                AAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCT                                                      <  SRR19973065.3502486/150‑1 (MQ=60)
                                                                                                        CGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCT                                              <  SRR19973065.2642647/150‑1 (MQ=60)
                                                                                                           ATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCC                                           >  SRR19973065.1893707/1‑150 (MQ=60)
                                                                                                           ATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCC                                           <  SRR19973065.2001802/150‑1 (MQ=60)
                                                                                                                GGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGA                                      >  SRR19973065.1893082/1‑150 (MQ=60)
                                                                                                                    TTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGA                                  >  SRR19973065.2284636/1‑150 (MQ=60)
                                                                                                                      GATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGT                                >  SRR19973065.3524441/1‑150 (MQ=60)
                                                                                                                      GATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGT                                <  SRR19973065.971746/150‑1 (MQ=60)
                                                                                                                      GATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGT                                >  SRR19973065.1208097/1‑150 (MQ=60)
                                                                                                                      GATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGT                                >  SRR19973065.1208285/1‑150 (MQ=60)
                                                                                                                      GATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGT                                >  SRR19973065.1208322/1‑150 (MQ=60)
                                                                                                                         CGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAT                             <  SRR19973065.3313620/150‑1 (MQ=60)
                                                                                                                          GTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATT                            >  SRR19973065.469574/1‑150 (MQ=60)
                                                                                                                           TTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTC                           <  SRR19973065.644448/150‑1 (MQ=60)
                                                                                                                              AAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTTACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGAGAGTTATTCCTG                        >  SRR19973065.470105/1‑150 (MQ=60)
                                                                                                                              AAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTG                        >  SRR19973065.1583678/1‑150 (MQ=60)
                                                                                                                                      TCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATA                >  SRR19973065.375514/1‑150 (MQ=60)
                                                                                                                                       CGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAC               <  SRR19973065.3447345/150‑1 (MQ=60)
                                                                                                                                        GATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACG              <  SRR19973065.481641/150‑1 (MQ=60)
                                                                                                                                         ATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGT             >  SRR19973065.2861687/1‑150 (MQ=60)
                                                                                                                                         ATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGT             >  SRR19973065.2861692/1‑150 (MQ=60)
                                                                                                                                          TGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTC            >  SRR19973065.2336957/1‑150 (MQ=60)
                                                                                                                                           GGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCG           <  SRR19973065.431779/150‑1 (MQ=60)
                                                                                                                                            GGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGC          <  SRR19973065.380572/150‑1 (MQ=60)
                                                                                                                                               GATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGG       >  SRR19973065.1535061/1‑150 (MQ=60)
                                                                                                                                                    TATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAA  >  SRR19973065.865365/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGCGTAA  >  CP009273/3789232‑3789529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑