Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,600,091 G→T 40.4% E262* (GAA→TAA)  lsrB → autoinducer 2‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,600,0910GT40.4% 210.4 / 194.8 188E262* (GAA→TAA) lsrBautoinducer 2‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (50/62);  new base T (37/39);  total (87/101)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 188 total aligned reads displayed.

TCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAG  >  CP009273/1599946‑1600237
                                                                                                                                                 |                                                                                                                                                  
  tcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAtt                                                                                                                                                <  2:2082216/148‑1 (MQ=255)
   cGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAttt                                                                                                                                               >  2:1056974/1‑148 (MQ=255)
     aCGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTgg                                                                                                                                             >  2:1301898/1‑148 (MQ=255)
      cGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGc                                                                                                                                            <  1:1177842/148‑1 (MQ=255)
           ttATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCtgtg                                                                                                                                       <  2:1419414/148‑1 (MQ=255)
            tATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTgg                                                                                                                                      <  1:2744102/148‑1 (MQ=255)
            tATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTgg                                                                                                                                      <  1:2085049/148‑1 (MQ=255)
            tATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGACAAATTTGATGAGCCCGTATGTATAGCGCGGCACGTTGAAAGAATTTGGCCtgtgt                                                                                                                                      >  1:1692442/1‑147 (MQ=255)
             aTCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTggg                                                                                                                                     <  1:1700757/148‑1 (MQ=255)
                  ccccGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGAtgtg                                                                                                                                <  2:1362624/148‑1 (MQ=255)
                  ccccGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGAtgtg                                                                                                                                <  2:1362635/148‑1 (MQ=255)
                   cccGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTgg                                                                                                                               >  1:588472/1‑148 (MQ=255)
                      gATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTc                                                                                                                            <  2:3409775/148‑1 (MQ=255)
                         gCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGAGGTGGTTCAGc                                                                                                                         >  1:1567818/1‑148 (MQ=255)
                           cAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCaa                                                                                                                       <  1:69807/148‑1 (MQ=255)
                              cGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGc                                                                                                                    <  1:2377886/148‑1 (MQ=255)
                                 ccTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCaaa                                                                                                                 <  2:2340053/148‑1 (MQ=255)
                                 ccTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCaaa                                                                                                                 <  1:2974706/148‑1 (MQ=255)
                                  cTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCaaaa                                                                                                                >  2:1687763/1‑148 (MQ=255)
                                   tGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAAt                                                                                                               >  2:732211/1‑148 (MQ=255)
                                   tGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAAt                                                                                                               >  2:1231370/1‑148 (MQ=255)
                                    gCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAAtt                                                                                                              <  2:2008423/148‑1 (MQ=255)
                                     cccGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAAttt                                                                                                             >  2:2834882/1‑148 (MQ=255)
                                      ccGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTc                                                                                                            <  1:689407/148‑1 (MQ=255)
                                         cTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAgt                                                                                                         <  2:2295747/148‑1 (MQ=255)
                                            ccGCACAAGTCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTa                                                                                                      >  1:1295007/1‑148 (MQ=255)
                                              gCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATg                                                                                                    <  2:696050/148‑1 (MQ=255)
                                              gCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATg                                                                                                    <  2:301657/148‑1 (MQ=255)
                                               cacaAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGt                                                                                                   <  2:2417178/148‑1 (MQ=255)
                                               cacaAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGt                                                                                                   <  2:3541818/148‑1 (MQ=255)
                                               cacaAGCCGCAGAAAACTTGAAAAATGACAAAGGAGCGAGTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGt                                                                                                   <  2:2417242/148‑1 (MQ=255)
                                                acaAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTc                                                                                                  >  1:842218/1‑148 (MQ=255)
                                                 caAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTcg                                                                                                 <  2:3200514/148‑1 (MQ=255)
                                                  aaGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTcgc                                                                                                >  1:1049255/1‑148 (MQ=255)
                                                    gCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCgg                                                                                              <  2:3512039/148‑1 (MQ=255)
                                                    gCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCgg                                                                                              <  2:2495214/148‑1 (MQ=255)
                                                    gCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCgg                                                                                              >  2:2986315/1‑148 (MQ=255)
                                                        cAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGc                                                                                          <  2:459114/148‑1 (MQ=255)
                                                        cAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGc                                                                                          >  2:2739314/1‑148 (MQ=255)
                                                          gAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCat                                                                                        <  1:233127/148‑1 (MQ=255)
                                                          gAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCat                                                                                        >  1:2052422/1‑148 (MQ=255)
                                                           aaacCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatt                                                                                       <  2:3136639/148‑1 (MQ=255)
                                                           aaaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatt                                                                                       >  1:3181703/1‑148 (MQ=255)
                                                           aaaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatt                                                                                       >  1:421455/1‑148 (MQ=255)
                                                              aCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattatt                                                                                    >  1:971303/1‑148 (MQ=255)
                                                              aCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattatt                                                                                    >  1:1968749/1‑148 (MQ=255)
                                                                ttGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGa                                                                                  <  2:676323/148‑1 (MQ=255)
                                                                 tGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGaa                                                                                 <  1:3557193/148‑1 (MQ=255)
                                                                     aaaTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGaaaaaa                                                                             <  1:637180/148‑1 (MQ=255)
                                                                     aaaTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGaaaaaa                                                                             <  1:636690/148‑1 (MQ=255)
                                                                       aTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGATCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAgg                                                                           <  2:2993236/148‑1 (MQ=255)
                                                                       aTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAgg                                                                           >  1:3322575/1‑148 (MQ=255)
                                                                        tGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGa                                                                          <  1:2869936/148‑1 (MQ=255)
                                                                        tGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGa                                                                          <  1:1304893/148‑1 (MQ=255)
                                                                         gACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGAt                                                                         >  1:2071126/1‑148 (MQ=255)
                                                                         gACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGAt                                                                         >  1:2071195/1‑148 (MQ=255)
                                                                          aCAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATc                                                                        >  2:1803672/1‑148 (MQ=255)
                                                                           cAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCa                                                                       <  1:2429874/148‑1 (MQ=255)
                                                                             aaGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAAt                                                                     <  1:1576797/148‑1 (MQ=255)
                                                                               gTAGCGATTGTCGGAGTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGa                                                                   <  2:87998/148‑1 (MQ=255)
                                                                                tAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGaa                                                                  <  2:1220155/148‑1 (MQ=255)
                                                                                tAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGaa                                                                  >  2:182185/1‑148 (MQ=255)
                                                                                    gATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACg                                                              >  1:1467403/1‑148 (MQ=255)
                                                                                     aTTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACgg                                                             >  1:2123592/1‑148 (MQ=255)
                                                                                       tGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                           <  2:2665146/148‑1 (MQ=255)
                                                                                         tCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGa                                                         >  2:802821/1‑148 (MQ=255)
                                                                                         tCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGa                                                         <  2:2101258/148‑1 (MQ=255)
                                                                                           ggATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACa                                                       <  2:2953888/148‑1 (MQ=255)
                                                                                             aTTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAg                                                     <  2:3259698/148‑1 (MQ=255)
                                                                                              ttCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGc                                                    <  2:3450707/148‑1 (MQ=255)
                                                                                                 aGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTgg                                                 >  2:368741/1‑148 (MQ=255)
                                                                                                  gTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                >  1:2279842/1‑148 (MQ=255)
                                                                                                   tACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGat                                               >  2:2118664/1‑148 (MQ=255)
                                                                                                      gCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATc                                            >  2:787408/1‑148 (MQ=255)
                                                                                                       ccAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCa                                           <  1:2467936/148‑1 (MQ=255)
                                                                                                         aaaTGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAg                                         <  1:1231336/148‑1 (MQ=255)
                                                                                                         aaaTGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAg                                         <  1:732398/148‑1 (MQ=255)
                                                                                                         aaaTGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAg                                         >  2:3040716/1‑148 (MQ=255)
                                                                                                            tgtgATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGc                                      >  1:3509303/1‑148 (MQ=255)
                                                                                                              tgATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATTTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGt                                    >  1:1951209/1‑148 (MQ=255)
                                                                                                                  gcgcCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGt                                <  2:562176/148‑1 (MQ=255)
                                                                                                                  gcgcCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTTGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGt                                <  2:2048596/148‑1 (MQ=255)
                                                                                                                     cccGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAg                             <  2:2433152/148‑1 (MQ=255)
                                                                                                                       cGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGt                           <  2:2876900/148‑1 (MQ=255)
                                                                                                                       cGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGt                           <  1:3505424/148‑1 (MQ=255)
                                                                                                                          aTGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                        <  2:1692467/148‑1 (MQ=255)
                                                                                                                          aTGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                        >  2:2136676/1‑148 (MQ=255)
                                                                                                                            gTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAg                      >  2:3046455/1‑148 (MQ=255)
                                                                                                                            gTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAg                      >  2:1635224/1‑148 (MQ=255)
                                                                                                                                agCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                  >  1:3078224/1‑148 (MQ=255)
                                                                                                                                       cACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAAc           >  1:1026124/1‑148 (MQ=255)
                                                                                                                                        aCGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACa          <  1:2834882/148‑1 (MQ=255)
                                                                                                                                           gTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCg       >  2:2368090/1‑148 (MQ=255)
                                                                                                                                           gTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCg       >  1:3433257/1‑148 (MQ=255)
                                                                                                                                            tGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCTGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGt      >  1:2886585/1‑148 (MQ=255)
                                                                                                                                            tGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGt      >  2:331652/1‑148 (MQ=255)
                                                                                                                                             gAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt     <  2:588472/148‑1 (MQ=255)
                                                                                                                                             gAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt     >  1:145744/1‑148 (MQ=255)
                                                                                                                                             gAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAACCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt     >  1:1387562/1‑148 (MQ=255)
                                                                                                                                                aGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAg  <  2:972613/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAG  >  CP009273/1599946‑1600237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A