Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,600,091 G→T 18.5% E262* (GAA→TAA)  lsrB → autoinducer 2‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,600,0910GT18.5% 665.6 / 93.2 237E262* (GAA→TAA) lsrBautoinducer 2‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (84/109);  new base T (15/29);  total (99/138)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 243 total aligned reads displayed.

ATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGG  >  CP009273/1599945‑1600238
                                                                                                                                                  |                                                                                                                                                   
aTCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGa                                                                                                                                                    >  2:3380034/1‑148 (MQ=255)
 tctcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGaa                                                                                                                                                   >  1:2510982/1‑148 (MQ=255)
 tctcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGaa                                                                                                                                                   <  1:2188922/148‑1 (MQ=255)
  ctcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAaataat                                                                                                                                                  >  1:173581/1‑148 (MQ=255)
  ctcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAt                                                                                                                                                  <  2:514932/148‑1 (MQ=255)
   tcGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAtt                                                                                                                                                 <  2:2785501/148‑1 (MQ=255)
    cGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAttt                                                                                                                                                <  2:1932498/148‑1 (MQ=255)
    cGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAAttt                                                                                                                                                <  2:2394950/148‑1 (MQ=255)
       cGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGc                                                                                                                                             <  2:2129905/148‑1 (MQ=255)
          cATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCtg                                                                                                                                          >  1:2123050/1‑148 (MQ=255)
            ttATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCACATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCtgtg                                                                                                                                        >  1:802317/1‑148 (MQ=255)
            ttATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCtgtg                                                                                                                                        >  2:2249809/1‑148 (MQ=255)
               tCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGa                                                                                                                                     <  2:1733408/148‑1 (MQ=255)
               tCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGa                                                                                                                                     <  2:3553961/148‑1 (MQ=255)
                 gCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGAtg                                                                                                                                   <  1:717783/148‑1 (MQ=255)
                 gCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGAtg                                                                                                                                   <  2:3592171/148‑1 (MQ=255)
                      cGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGtt                                                                                                                              >  2:3523563/1‑148 (MQ=255)
                              aCGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAgg                                                                                                                      <  2:2886413/148‑1 (MQ=255)
                                   cTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCaaaa                                                                                                                 >  1:1060726/1‑148 (MQ=255)
                                     gCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTCGAGCGCGGCACGGTGACAGAATTTGGCGTGTGGGAGGTGGTTCAGCAAGGCAAAAtt                                                                                                               >  1:973200/1‑148 (MQ=255)
                                       ccGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTc                                                                                                             <  2:3737962/148‑1 (MQ=255)
                                        cGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCa                                                                                                            <  1:1876651/148‑1 (MQ=255)
                                         gCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAg                                                                                                           <  2:3088769/148‑1 (MQ=255)
                                         gCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAg                                                                                                           <  1:1943910/148‑1 (MQ=255)
                                            gCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAgtgt                                                                                                        >  2:813449/1‑148 (MQ=255)
                                             ccGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGAGTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTa                                                                                                       <  1:2207435/148‑1 (MQ=255)
                                                  caAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTAAGCCAAATGGGATGCGCCCGTATGTAGAGCGCGGAACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATATAAGTGTATGtag                                                                                                  >  2:1271134/1‑146 (MQ=255)
                                                     gtcGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCgg                                                                                               >  2:2630226/3‑148 (MQ=255)
                                                     gCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCgg                                                                                               <  1:2417107/148‑1 (MQ=255)
                                                           gAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCat                                                                                         <  1:1067656/148‑1 (MQ=255)
                                                            aaaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatt                                                                                        <  2:1359190/148‑1 (MQ=255)
                                                            aaaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatt                                                                                        <  1:845189/148‑1 (MQ=255)
                                                             aaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatta                                                                                       <  2:173589/148‑1 (MQ=255)
                                                             aaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatta                                                                                       <  1:1172748/148‑1 (MQ=255)
                                                             aaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatta                                                                                       <  2:297451/148‑1 (MQ=255)
                                                             aaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatta                                                                                       <  2:1070828/148‑1 (MQ=255)
                                                             aaaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCatta                                                                                       <  2:2694585/148‑1 (MQ=255)
                                                              aaCTTTAAAAATGACAAAGTAGCGAGTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                                                                                      <  1:1628616/148‑1 (MQ=255)
                                                              aaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                                                                                      <  1:3485843/148‑1 (MQ=255)
                                                              aaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                                                                                      <  2:692904/148‑1 (MQ=255)
                                                              aaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                                                                                      <  1:3608332/148‑1 (MQ=255)
                                                              aaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                                                                                      <  1:1491714/148‑1 (MQ=255)
                                                              aaCTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                                                                                      <  1:1519140/148‑1 (MQ=255)
                                                                cTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTg                                                                                    >  1:516674/1‑148 (MQ=255)
                                                                 ttGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGa                                                                                   <  2:626807/148‑1 (MQ=255)
                                                                  tGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGaa                                                                                  >  2:1606150/1‑148 (MQ=255)
                                                                      aaaTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGaaaaaa                                                                              >  2:1848661/1‑148 (MQ=255)
                                                                      aaaTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGaaaaaa                                                                              >  2:3202410/1‑148 (MQ=255)
                                                                        aTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAgg                                                                            <  2:1193248/148‑1 (MQ=255)
                                                                        aTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAgg                                                                            <  1:3093474/148‑1 (MQ=255)
                                                                        aTGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAgg                                                                            <  1:3472215/148‑1 (MQ=255)
                                                                        aTGACAAAGTAGCGAGTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAgg                                                                            <  1:2055450/148‑1 (MQ=255)
                                                                         tGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGa                                                                           <  1:3117688/148‑1 (MQ=255)
                                                                         tGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGa                                                                           <  2:1095295/148‑1 (MQ=255)
                                                                         tGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGa                                                                           >  2:2141582/1‑148 (MQ=255)
                                                                          gACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGAt                                                                          <  2:1272158/148‑1 (MQ=255)
                                                                            cAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCa                                                                        <  1:2743855/148‑1 (MQ=255)
                                                                            cAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCa                                                                        <  2:442110/148‑1 (MQ=255)
                                                                             aaaGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCaa                                                                       <  2:1317096/148‑1 (MQ=255)
                                                                              aaGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAAt                                                                      <  2:2914692/148‑1 (MQ=255)
                                                                              aaGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAAt                                                                      <  1:161886/148‑1 (MQ=255)
                                                                               aGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATg                                                                     >  1:1683918/1‑148 (MQ=255)
                                                                                  aGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGaaa                                                                  <  2:1632256/148‑1 (MQ=255)
                                                                                   gCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGaaaa                                                                 <  2:1420768/148‑1 (MQ=255)
                                                                                   gCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGaaaa                                                                 <  2:1923482/148‑1 (MQ=255)
                                                                                      aTTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACgg                                                              >  2:3057672/1‑148 (MQ=255)
                                                                                        tGTCGGATTCAGTACTCCAAAAGTGATGCGCACGTATGTAGATCGCTGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTTTATGTAGCGGATGCATTATTGAAAAAAGGATCAATGAAAActcgc                                                            >  2:1050381/1‑144 (MQ=255)
                                                                                        tGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCTCGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                            >  2:248812/1‑148 (MQ=255)
                                                                                        tGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                            >  2:1945017/1‑148 (MQ=255)
                                                                                          tCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCAATATTGAAAAAAGGATCAATGAAAACGGGCGa                                                          >  2:828461/1‑148 (MQ=255)
                                                                                          tCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGa                                                          >  2:3668038/1‑148 (MQ=255)
                                                                                              aTTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAg                                                      >  1:3411575/1‑148 (MQ=255)
                                                                                                tCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCt                                                    >  1:229023/1‑148 (MQ=255)
                                                                                                 cAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTg                                                   >  2:1883799/1‑148 (MQ=255)
                                                                                                   gTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                 <  2:2281946/148‑1 (MQ=255)
                                                                                                    tACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGat                                                >  2:2208511/1‑148 (MQ=255)
                                                                                                      cGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGatat                                              <  1:3244899/148‑1 (MQ=255)
                                                                                                         cAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCaa                                           <  1:2989213/148‑1 (MQ=255)
                                                                                                         cAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCaa                                           <  1:185786/148‑1 (MQ=255)
                                                                                                           aaTGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAgg                                         <  1:2675157/148‑1 (MQ=255)
                                                                                                             tgtgATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGc                                       >  1:3512267/1‑148 (MQ=255)
                                                                                                                gATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCACAATTTCAGTGTATGTCGCGGATGCATGAATGAAAAAAGGATCAATGACAACGGGCGACAAGATGGATATCAAGGGCGTa                                    >  2:3524242/1‑148 (MQ=255)
                                                                                                                gATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTa                                    >  1:2774754/1‑148 (MQ=255)
                                                                                                                        cGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGt                            >  1:1234021/1‑148 (MQ=255)
                                                                                                                           aTGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                         <  1:2448459/148‑1 (MQ=255)
                                                                                                                           aTGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                         >  2:3797445/1‑148 (MQ=255)
                                                                                                                           aTGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGa                         <  1:271075/148‑1 (MQ=255)
                                                                                                                            tGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                        <  2:2049881/148‑1 (MQ=255)
                                                                                                                             gTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAg                       >  2:1604638/1‑148 (MQ=255)
                                                                                                                               agagCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtc                     <  1:664197/148‑1 (MQ=255)
                                                                                                                               agagCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtc                     <  2:1448529/148‑1 (MQ=255)
                                                                                                                                gagCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtct                    <  1:2565144/148‑1 (MQ=255)
                                                                                                                                gagCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtct                    <  2:3675110/148‑1 (MQ=255)
                                                                                                                                       gCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaaa             <  2:755677/148‑1 (MQ=255)
                                                                                                                                       gCACGGTGAAATAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaaa             <  2:758672/148‑1 (MQ=255)
                                                                                                                                          cGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAg          >  1:424773/1‑148 (MQ=255)
                                                                                                                                            gTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCg        <  1:3315932/148‑1 (MQ=255)
                                                                                                                                              gAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGtt      <  1:1235712/148‑1 (MQ=255)
                                                                                                                                                aaGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCa    >  1:2140257/1‑148 (MQ=255)
                                                                                                                                                  gAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAgg  >  1:826486/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGG  >  CP009273/1599945‑1600238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A