Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F368 I0 R1	1472	214.4	7116750	95.9%	6824963	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,621,733	T→A	9.0%	intergenic (‑96/‑41)	dcp ← / → ydfG	dipeptidyl carboxypeptidase II/NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,621,733	0	T	A	9.0%	698.8 / 46.9	232	intergenic (‑96/‑41)	dcp/ydfG	dipeptidyl carboxypeptidase II/NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase
Reads supporting (aligned to +/- strand): ref base T (104/107); new base A (11/10); total (115/117)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 233 total aligned reads displayed.

GAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAAC  >  CP009273/1621586‑1621879

 aGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTg                                                                                                                                                   <  2:848745/148‑1 (MQ=255)

 aGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTg                                                                                                                                                   <  2:543318/148‑1 (MQ=255)

  gCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGc                                                                                                                                                  >  1:1117231/1‑148 (MQ=255)

   ccAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCt                                                                                                                                                 >  1:593020/1‑148 (MQ=255)

   ccAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCt                                                                                                                                                 >  1:2377700/1‑148 (MQ=255)

   ccAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCt                                                                                                                                                 <  2:2724140/148‑1 (MQ=255)

      gATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATg                                                                                                                                              <  1:2927879/148‑1 (MQ=255)

      gATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATg                                                                                                                                              <  1:2464315/148‑1 (MQ=255)

        tACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGtt                                                                                                                                            >  1:1186545/1‑148 (MQ=255)

           ggCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTAtt                                                                                                                                         <  1:2529947/148‑1 (MQ=255)

             cAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGa                                                                                                                                       >  1:48666/1‑148 (MQ=255)

             cAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGa                                                                                                                                       >  1:1040642/1‑148 (MQ=255)

               gtgtGCTTTGCGCAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGaca                                                                                                                                     <  2:2656556/148‑1 (MQ=255)

                 gtgCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGacaca                                                                                                                                   >  2:462360/1‑148 (MQ=255)

                 gtgCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGacaca                                                                                                                                   <  1:2637953/148‑1 (MQ=255)

                   gCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGAcacaca                                                                                                                                 >  1:1845147/1‑148 (MQ=255)

                   gCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGAcacaca                                                                                                                                 >  1:3277333/1‑148 (MQ=255)

                   gCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGCAGTGCAATCTTGCAATCCAGTATTACAATCTCTTAAGCATCCCTTGCTATGTTATTGAcacaca                                                                                                                                 >  1:665708/1‑148 (MQ=255)

                    cTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACaa                                                                                                                                >  2:766856/1‑148 (MQ=255)

                      ttGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACaaaa                                                                                                                              <  1:3073726/148‑1 (MQ=255)

                      ttGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACaaaa                                                                                                                              <  1:1261867/148‑1 (MQ=255)

                       tGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAg                                                                                                                             >  1:624316/1‑148 (MQ=255)

                       tGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAg                                                                                                                             >  1:624521/1‑148 (MQ=255)

                          acaAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGt                                                                                                                          <  2:1208396/148‑1 (MQ=255)

                           caAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGtt                                                                                                                         >  2:1068092/1‑148 (MQ=255)

                              ggaaaggaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAg                                                                                                                      >  2:2961888/1‑148 (MQ=255)

                               gaaaggaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAgg                                                                                                                     <  2:1372414/148‑1 (MQ=255)

                                    gaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAg                                                                                                                >  1:810876/1‑148 (MQ=255)

                                    gaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAg                                                                                                                >  2:1705460/1‑148 (MQ=255)

                                    gaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAg                                                                                                                >  2:2572074/1‑148 (MQ=255)

                                      ttCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTg                                                                                                              >  1:3398519/1‑148 (MQ=255)

                                      ttCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTg                                                                                                              >  2:1177048/1‑148 (MQ=255)

                                      ttCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTg                                                                                                              >  2:155945/1‑148 (MQ=255)

                                      ttCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTg                                                                                                              >  2:2457380/1‑148 (MQ=255)

                                      ttCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTg                                                                                                              >  2:3372459/1‑148 (MQ=255)

                                       tCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTga                                                                                                             <  2:727246/148‑1 (MQ=255)

                                        cATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTgag                                                                                                            <  2:1843960/148‑1 (MQ=255)

                                         aTTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTgaga                                                                                                           >  1:875536/1‑148 (MQ=255)

                                           tgttgtCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAgatg                                                                                                         <  2:407607/148‑1 (MQ=255)

                                           tgttgtCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGGGAgatg                                                                                                         >  1:279239/1‑148 (MQ=255)

                                            gttgtCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAgatga                                                                                                        <  1:572173/148‑1 (MQ=255)

                                             ttgtCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAgatgat                                                                                                       <  2:2376189/148‑1 (MQ=255)

                                                tCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGt                                                                                                    <  2:3221326/148‑1 (MQ=255)

                                                 cATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGAACGtt                                                                                                   >  2:534598/1‑148 (MQ=255)

                                                    tttCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTa                                                                                                >  2:522550/1‑148 (MQ=255)

                                                            ccTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTgg                                                                                        >  2:1792354/1‑148 (MQ=255)

                                                            ccTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTgg                                                                                        >  2:1616030/1‑148 (MQ=255)

                                                            ccTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTgg                                                                                        <  1:2891368/148‑1 (MQ=255)

                                                             cTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGa                                                                                       >  2:2817271/1‑148 (MQ=255)

                                                              tGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAg                                                                                      >  2:1121179/1‑148 (MQ=255)

                                                               gAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGc                                                                                     >  2:1123706/1‑148 (MQ=255)

                                                                 aCGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCaa                                                                                   >  2:621611/1‑148 (MQ=255)

                                                                 aCGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCaa                                                                                   <  2:2280548/148‑1 (MQ=255)

                                                                  cgcgAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAAc                                                                                  >  1:3342002/1‑148 (MQ=255)

                                                                       ggTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAg                                                                             >  1:3035143/1‑148 (MQ=255)

                                                                        gtgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAgg                                                                            >  2:47434/1‑148 (MQ=255)

                                                                        gtgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAgg                                                                            >  2:48154/1‑148 (MQ=255)

                                                                         tgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGt                                                                           <  2:386478/148‑1 (MQ=255)

                                                                           ttCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGttt                                                                         <  2:315149/148‑1 (MQ=255)

                                                                            tCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtttt                                                                        <  1:3410195/148‑1 (MQ=255)

                                                                             ccATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTg                                                                       <  1:507431/148‑1 (MQ=255)

                                                                                  gCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGAAACTCATATGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGaa                                                                  <  1:3545941/148‑1 (MQ=255)

                                                                                    gTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATg                                                                <  2:1991457/148‑1 (MQ=255)

                                                                                     tAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGc                                                               >  2:2821336/1‑148 (MQ=255)

                                                                                     tAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGc                                                               <  1:1293377/148‑1 (MQ=255)

                                                                                     tAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGc                                                               <  1:1255561/148‑1 (MQ=255)

                                                                                      aGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCa                                                              <  2:1911579/148‑1 (MQ=255)

                                                                                       ggCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCAt                                                             >  1:1923982/1‑148 (MQ=255)

                                                                                        gCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCAtt                                                            >  1:124544/1‑148 (MQ=255)

                                                                                         cTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTa                                                           >  1:3318190/1‑148 (MQ=255)

                                                                                          ttACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTAc                                                          <  1:414868/148‑1 (MQ=255)

                                                                                          ttACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTAc                                                          <  1:1454608/148‑1 (MQ=255)

                                                                                               gATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACtcgtc                                                     >  1:2192429/1‑148 (MQ=255)

                                                                                                     ggAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTa                                               <  2:3320965/148‑1 (MQ=255)

                                                                                                          gCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTcaa                                          <  2:3298191/148‑1 (MQ=255)

                                                                                                          gCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTcaa                                          >  1:2262853/1‑148 (MQ=255)

                                                                                                           cAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTcaac                                         >  2:3446566/1‑148 (MQ=255)

                                                                                                              tCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTGACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAg                                      <  2:2591858/148‑1 (MQ=255)

                                                                                                              tCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTGTTATTCAACAAg                                      >  2:2780946/1‑148 (MQ=255)

                                                                                                                     aTCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATaa                               <  1:1483612/148‑1 (MQ=255)

                                                                                                                       ccAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAg                             <  1:2079859/148‑1 (MQ=255)

                                                                                                                        cAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGt                            >  1:585324/1‑148 (MQ=255)

                                                                                                                        cAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGt                            <  2:3234924/148‑1 (MQ=255)

                                                                                                                        cAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTGAACAAGGGCATAAAGt                            >  1:1615126/1‑148 (MQ=255)

                                                                                                                        cAGTATGACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGACTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGt                            <  2:2031020/148‑1 (MQ=255)

                                                                                                                          gTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTAAACAAGGGCATAAAGTTa                          >  1:1883985/1‑148 (MQ=255)

                                                                                                                           tATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTAt                         >  2:745877/1‑148 (MQ=255)

                                                                                                                             ttACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCg                       <  1:1616030/148‑1 (MQ=255)

                                                                                                                             ttACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCg                       >  2:2744839/1‑148 (MQ=255)

                                                                                                                             ttACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCg                       <  1:1792354/148‑1 (MQ=255)

                                                                                                                                cccGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGTAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCa                    <  1:462360/148‑1 (MQ=255)

                                                                                                                                cccGCTCTTAAGCATCCCGTGCTATGTTATTGACACAAAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCa                    <  2:665708/148‑1 (MQ=255)

                                                                                                                                            cATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGcc        <  1:1705460/148‑1 (MQ=255)

                                                                                                                                              tCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCCTAAAGTTATCGCCACTGGCCGTCGCCAg      >  1:3244498/1‑148 (MQ=255)

                                                                                                                                               cccGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAgg     >  1:2441891/1‑148 (MQ=255)

                                                                                                                                               cccGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAgg     >  1:2679127/1‑148 (MQ=255)

                                                                                                                                               cccGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAgg     >  1:1498746/1‑148 (MQ=255)

                                                                                                                                                ccGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGa    <  2:2066046/148‑1 (MQ=255)

                                                                                                                                                ccGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGa    <  1:369588/148‑1 (MQ=255)

                                                                                                                                                 cGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTAAACAAGGGCAGAAAGTTATAGCCACTGGCCGTCGCCAGGaa   >  1:71943/1‑148 (MQ=255)

GAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAAC  >  CP009273/1621586‑1621879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A