Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,621,733 T→A 26.0% intergenic (‑96/‑41) dcp ← / → ydfG dipeptidyl carboxypeptidase II/NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,621,7330TA26.0% 660.6 / 272.4 368intergenic (‑96/‑41)dcp/ydfGdipeptidyl carboxypeptidase II/NADP‑dependent 3‑hydroxy acid dehydrogenase; malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (132/140);  new base A (44/52);  total (176/192)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 372 total aligned reads displayed.

GGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGT  >  CP009273/1621584‑1621882
                                                                                                                                                     |                                                                                                                                                     
gggAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGt                                                                                                                                                       >  1:1698662/1‑150 (MQ=255)
 ggAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTg                                                                                                                                                      >  1:1093340/1‑150 (MQ=255)
 ggAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTg                                                                                                                                                      >  2:3090137/1‑150 (MQ=255)
     ccAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTAt                                                                                                                                                  >  1:575825/1‑150 (MQ=255)
        gATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGtt                                                                                                                                               <  2:5168026/150‑1 (MQ=255)
        gATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGtt                                                                                                                                               <  2:2179068/150‑1 (MQ=255)
           aCGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTAtt                                                                                                                                            <  1:4116066/150‑1 (MQ=255)
            cGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTg                                                                                                                                           >  1:352841/1‑150 (MQ=255)
             ggCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGa                                                                                                                                          >  1:3631401/1‑150 (MQ=255)
               cAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGaca                                                                                                                                        >  2:2593282/1‑150 (MQ=255)
                aGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGacac                                                                                                                                       >  2:4277949/1‑150 (MQ=255)
                      cTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACaaaa                                                                                                                                 <  1:4486225/150‑1 (MQ=255)
                      cTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACaaaa                                                                                                                                 <  1:3208044/150‑1 (MQ=255)
                       tttGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAg                                                                                                                                <  2:3633649/150‑1 (MQ=255)
                          gCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGt                                                                                                                             >  1:2711491/1‑150 (MQ=255)
                             caAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGa                                                                                                                          <  1:929701/150‑1 (MQ=255)
                              aaggaaaggaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAg                                                                                                                         >  1:3128219/1‑150 (MQ=255)
                                   aaggaTTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACa                                                                                                                    <  1:1033941/150‑1 (MQ=255)
                                        ttCAGTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTgag                                                                                                               <  2:4209910/150‑1 (MQ=255)
                                           aTTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAgatg                                                                                                            <  1:2307191/150‑1 (MQ=255)
                                           aTTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAgatg                                                                                                            >  2:2360087/1‑150 (MQ=255)
                                           aTTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAgatg                                                                                                            >  1:4692090/1‑150 (MQ=255)
                                           aTTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAgatg                                                                                                            <  2:801011/150‑1 (MQ=255)
                                                 gtCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGtt                                                                                                      >  1:209252/1‑150 (MQ=255)
                                                  tCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGttt                                                                                                     <  2:3364863/150‑1 (MQ=255)
                                                   cATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGtttt                                                                                                    <  2:1514583/150‑1 (MQ=255)
                                                   cATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGtttt                                                                                                    >  1:3800995/1‑150 (MQ=255)
                                                    aTTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTa                                                                                                   >  2:3488214/1‑150 (MQ=255)
                                                    aTTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTa                                                                                                   >  2:228935/1‑150 (MQ=255)
                                                    aTTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTa                                                                                                   >  2:968265/1‑150 (MQ=255)
                                                     ttttCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAg                                                                                                  <  2:2859961/150‑1 (MQ=255)
                                                        tcttctCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTaa                                                                                               >  1:5188274/1‑150 (MQ=255)
                                                         cttctCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAAc                                                                                              <  2:4071588/150‑1 (MQ=255)
                                                          ttctCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACt                                                                                             <  2:4152389/150‑1 (MQ=255)
                                                          ttctCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACt                                                                                             <  2:4472934/150‑1 (MQ=255)
                                                          ttctCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACt                                                                                             >  2:3372961/1‑150 (MQ=255)
                                                          ttctCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACt                                                                                             <  1:97488/150‑1 (MQ=255)
                                                              ccTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAg                                                                                         >  2:1002206/1‑150 (MQ=255)
                                                                 gAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCaa                                                                                      <  2:4659850/150‑1 (MQ=255)
                                                                  aaCGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAAc                                                                                     <  1:4369403/150‑1 (MQ=255)
                                                                  aaCGCGAGGTGATCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAAc                                                                                     <  2:2649884/150‑1 (MQ=255)
                                                                     gcgAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGc                                                                                  <  1:4821238/150‑1 (MQ=255)
                                                                     gcgAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGc                                                                                  <  1:1962587/150‑1 (MQ=255)
                                                                      cgAGGTGGTCCAGAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTTACACACAAAAGCGTTGAGGAACAGTGAGATTATCGTTTTAGTTACTGGAGCAACGGCa                                                                                 <  2:1144302/150‑1 (MQ=255)
                                                                       gAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAg                                                                                <  1:367743/150‑1 (MQ=255)
                                                                        aGGTGGTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAgg                                                                               <  1:5323541/150‑1 (MQ=255)
                                                                         ggTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGt                                                                              >  1:4962896/1‑150 (MQ=255)
                                                                          gtgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtt                                                                             >  2:1702774/1‑150 (MQ=255)
                                                                          gtgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtt                                                                             <  2:1930338/150‑1 (MQ=255)
                                                                           tgtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGttt                                                                            <  1:893584/150‑1 (MQ=255)
                                                                            gtTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGtttt                                                                           <  1:3023715/150‑1 (MQ=255)
                                                                              tacATAGCGTAGGATTACTGATAACGGAGTGCAATCTTGAAATCCAGTATTACACGCTCTTAAGCATCCAGTGCTATGTTATTGACACACAAAATCGTTGAGGAACAGTGAGATGATCTTTTTAGTAACTGTAGCAACTGCAGGTTTTgg                                                                         >  2:3581822/3‑150 (MQ=255)
                                                                              tCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTgg                                                                         <  2:1633463/150‑1 (MQ=255)
                                                                               ccATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTAAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGt                                                                        >  2:849774/1‑150 (MQ=255)
                                                                                cATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTg                                                                       >  2:4936738/1‑150 (MQ=255)
                                                                                cATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTg                                                                       <  2:742787/150‑1 (MQ=255)
                                                                                    gCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATg                                                                   <  1:1363034/150‑1 (MQ=255)
                                                                                    gCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATg                                                                   <  1:2534010/150‑1 (MQ=255)
                                                                                     cGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGc                                                                  >  2:1445691/1‑150 (MQ=255)
                                                                                     cGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGc                                                                  >  1:2945563/1‑150 (MQ=255)
                                                                                        aGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCTTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCAtt                                                               >  1:4446847/1‑150 (MQ=255)
                                                                                        aGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCAtt                                                               <  1:418539/150‑1 (MQ=255)
                                                                                          gCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTAc                                                             <  1:4650674/150‑1 (MQ=255)
                                                                                           cTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACt                                                            <  2:107502/150‑1 (MQ=255)
                                                                                           cTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTCTTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGGTGTAGTAACTGGAGCAACGGCAGGTTTTGGTGACTGCATTACt                                                            >  2:1606514/1‑150 (MQ=255)
                                                                                            ttACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACtc                                                           >  1:2445335/1‑150 (MQ=255)
                                                                                            ttACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACtc                                                           <  1:4300093/150‑1 (MQ=255)
                                                                                               cTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACtcgtc                                                        <  1:3366380/150‑1 (MQ=255)
                                                                                                tGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACtcgtcg                                                       <  2:4108655/150‑1 (MQ=255)
                                                                                                  aTAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGtt                                                     >  1:1460383/1‑150 (MQ=255)
                                                                                                  aTAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGtt                                                     >  2:3900858/1‑150 (MQ=255)
                                                                                                  aTAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGtt                                                     >  1:1880357/1‑150 (MQ=255)
                                                                                                  aTAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGtt                                                     <  2:366510/150‑1 (MQ=255)
                                                                                                     aCGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTa                                                  >  2:1278959/1‑150 (MQ=255)
                                                                                                        gAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTc                                               <  1:2593282/150‑1 (MQ=255)
                                                                                                          gTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTcaa                                             <  1:4872956/150‑1 (MQ=255)
                                                                                                           tGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTcaac                                            <  2:1719792/150‑1 (MQ=255)
                                                                                                              aaTCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAg                                         >  2:4806371/1‑150 (MQ=255)
                                                                                                              aaTCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAg                                         <  2:4744249/150‑1 (MQ=255)
                                                                                                              aaTCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAg                                         <  2:2091862/150‑1 (MQ=255)
                                                                                                               aTCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGCAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAAAAAgg                                        >  2:2458641/1‑150 (MQ=255)
                                                                                                                  ttGCAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCa                                     <  1:760106/150‑1 (MQ=255)
                                                                                                                    gCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATa                                   <  1:1296895/150‑1 (MQ=255)
                                                                                                                     cAATCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATaa                                  <  2:3962933/150‑1 (MQ=255)
                                                                                                                        tCCAGTATTACCCGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGt                               <  2:4721968/150‑1 (MQ=255)
                                                                                                                           aGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTAt                            <  1:2191995/150‑1 (MQ=255)
                                                                                                                             tATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCg                          >  2:2835428/1‑150 (MQ=255)
                                                                                                                               ttACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGcc                        >  1:2024813/1‑150 (MQ=255)
                                                                                                                                  cccGCTCTTAAGCATCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACt                     <  2:2544096/150‑1 (MQ=255)
                                                                                                                                   ccGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTg                    <  2:3257097/150‑1 (MQ=255)
                                                                                                                                    cGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTgg                   >  2:2752256/1‑150 (MQ=255)
                                                                                                                                      ctctTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGcc                 >  2:4595758/1‑150 (MQ=255)
                                                                                                                                      ctctTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGcc                 <  1:3090137/150‑1 (MQ=255)
                                                                                                                                       tctTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCg                >  1:2608452/1‑150 (MQ=255)
                                                                                                                                              cATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAg         >  2:5051002/1‑150 (MQ=255)
                                                                                                                                               aTCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAgg        >  2:4765119/1‑150 (MQ=255)
                                                                                                                                               aTCCCGAGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAgg        >  2:677146/1‑150 (MQ=255)
                                                                                                                                                  ccGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAAc     <  2:2390962/150‑1 (MQ=255)
                                                                                                                                                   cGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACg    <  1:5116970/150‑1 (MQ=255)
                                                                                                                                                    gTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACgg   <  2:3128219/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGAGCCAGATACGGCAGTGTGCTTTGCACAAGGAAAGGATTCATTGTTGTCATTTTCTTCTCCTGAACGCGAGGTGTTCCATAGCGTAGGCTTACTGATAACGGAGTGCAATCTTGCAATCCAGTATTACCCGCTCTTAAGCATCCCGTGCTATGTTATTGACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGT  >  CP009273/1621584‑1621882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A