Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,303,070 T→G 100% Q272P (CAG→CCG)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,303,0700TG99.0% 749.5 / ‑3.9 184Q272P (CAG→CCG) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (96/86);  total (98/86)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

GGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCG  >  CP009273/2302925‑2303213
                                                                                                                                                 |                                                                                                                                               
gggTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGa                                                                                                                                               >  1:233381/1‑148 (MQ=255)
gggTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGa                                                                                                                                               >  1:1293000/1‑148 (MQ=255)
 ggTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGaa                                                                                                                                              >  2:3388156/1‑148 (MQ=255)
  gTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGaaa                                                                                                                                             >  1:249957/1‑148 (MQ=255)
  gTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGaaa                                                                                                                                             <  1:1818007/148‑1 (MQ=255)
  gTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGaaa                                                                                                                                             <  1:1817677/148‑1 (MQ=255)
  gTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGaaa                                                                                                                                             <  1:17357/148‑1 (MQ=255)
    attatGATAGCCAGCTTGTAGGCGGCACAGGCGCTGGAAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGATAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAg                                                                                                                                           >  2:2804466/1‑148 (MQ=255)
     ttatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGc                                                                                                                                          <  2:2080422/148‑1 (MQ=255)
      tatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCa                                                                                                                                         >  2:2957249/1‑148 (MQ=255)
      tatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCa                                                                                                                                         >  1:3291014/1‑148 (MQ=255)
      tatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCa                                                                                                                                         >  1:2511802/1‑148 (MQ=255)
      tatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCa                                                                                                                                         <  1:2404192/148‑1 (MQ=255)
        tGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGt                                                                                                                                       <  2:2749443/148‑1 (MQ=255)
         gATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTg                                                                                                                                      >  1:3435779/1‑148 (MQ=255)
         gATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTg                                                                                                                                      >  1:1032294/1‑148 (MQ=255)
            aGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcg                                                                                                                                   >  2:1662647/1‑148 (MQ=255)
              ccAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcgcg                                                                                                                                 >  2:2755398/1‑148 (MQ=255)
              ccAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcgcg                                                                                                                                 >  2:1437777/1‑148 (MQ=255)
               cAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGGAAAGCAGTGCCGCGa                                                                                                                                <  2:3512143/148‑1 (MQ=255)
                  cTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATg                                                                                                                             >  1:3428009/1‑148 (MQ=255)
                   ttgttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGt                                                                                                                            <  2:3222444/148‑1 (MQ=255)
                    tgttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTc                                                                                                                           >  2:2363620/1‑148 (MQ=255)
                    tgttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTc                                                                                                                           >  1:692930/1‑148 (MQ=255)
                     gttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTcc                                                                                                                          <  1:1073316/148‑1 (MQ=255)
                         gcggcACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCg                                                                                                                      >  2:1549916/1‑148 (MQ=255)
                         gcggcACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCg                                                                                                                      >  2:1550677/1‑148 (MQ=255)
                         gcggcACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCg                                                                                                                      <  1:2627998/148‑1 (MQ=255)
                          cggcACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCgg                                                                                                                     >  1:1546613/1‑148 (MQ=255)
                              acaGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAAc                                                                                                                 <  2:576510/148‑1 (MQ=255)
                                aGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGt                                                                                                               >  1:2622457/1‑148 (MQ=255)
                                aGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGt                                                                                                               <  2:2423907/148‑1 (MQ=255)
                                aGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGt                                                                                                               >  1:1940675/1‑148 (MQ=255)
                                aGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGt                                                                                                               <  2:695099/148‑1 (MQ=255)
                                aGGCGCGGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGt                                                                                                               <  2:695087/148‑1 (MQ=255)
                                 ggCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTc                                                                                                              >  1:2930559/1‑148 (MQ=255)
                                   cgcTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAg                                                                                                            >  1:501402/1‑148 (MQ=255)
                                        gCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAgcggcg                                                                                                       >  2:1512856/1‑148 (MQ=255)
                                         cAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGt                                                                                                      <  1:1874163/148‑1 (MQ=255)
                                         cAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGt                                                                                                      >  2:3407432/1‑148 (MQ=255)
                                           aCGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAt                                                                                                    <  1:2219185/148‑1 (MQ=255)
                                           aCGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAt                                                                                                    >  1:3180469/1‑148 (MQ=255)
                                           aCGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAt                                                                                                    <  2:3502293/148‑1 (MQ=255)
                                           aCGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAt                                                                                                    >  1:202307/1‑148 (MQ=255)
                                             ggCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATcg                                                                                                  >  2:725809/1‑148 (MQ=255)
                                              gCCCGTGCCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATcgc                                                                                                 >  2:333138/1‑148 (MQ=255)
                                               cccGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATcgcg                                                                                                >  2:1497599/1‑148 (MQ=255)
                                                ccGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGt                                                                                               <  1:1658984/148‑1 (MQ=255)
                                                 cGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGtt                                                                                              >  2:1864995/1‑148 (MQ=255)
                                                 cGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGtt                                                                                              >  2:891920/1‑148 (MQ=255)
                                                   tACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGttga                                                                                            <  2:767131/148‑1 (MQ=255)
                                                    aCCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGttgat                                                                                           <  2:3519762/148‑1 (MQ=255)
                                                       gCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGttgattga                                                                                        <  1:3509705/148‑1 (MQ=255)
                                                        cTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAg                                                                                       <  1:1260084/148‑1 (MQ=255)
                                                         tttCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGACTGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAgg                                                                                      >  2:2574378/1‑148 (MQ=255)
                                                             ggTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTgg                                                                                  <  2:386209/148‑1 (MQ=255)
                                                               tttGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCg                                                                                >  2:177925/1‑148 (MQ=255)
                                                                ttGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGa                                                                               >  2:1630719/1‑148 (MQ=255)
                                                                 tGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGAt                                                                              <  1:1372030/148‑1 (MQ=255)
                                                                          cGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACg                                                                     >  1:1419458/1‑148 (MQ=255)
                                                                              aGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGcaca                                                                 <  2:1269913/148‑1 (MQ=255)
                                                                               gCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACAt                                                                >  1:356639/1‑148 (MQ=255)
                                                                               gCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGGATCGCGGTGATTGAGGCTGGCGATCACTTCACGCACAt                                                                >  2:2059961/1‑148 (MQ=255)
                                                                                ccAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATg                                                               >  1:2658923/1‑148 (MQ=255)
                                                                                ccAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATg                                                               >  2:2885003/1‑148 (MQ=255)
                                                                                  aGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGtt                                                             >  2:1162833/1‑148 (MQ=255)
                                                                                   gTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTg                                                            <  2:704865/148‑1 (MQ=255)
                                                                                   gTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTg                                                            <  2:761290/148‑1 (MQ=255)
                                                                                         gATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGaaa                                                      <  2:160491/148‑1 (MQ=255)
                                                                                          aTAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAAc                                                     >  1:2689599/1‑148 (MQ=255)
                                                                                             aCTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATc                                                  <  1:891924/148‑1 (MQ=255)
                                                                                             aCTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATc                                                  <  1:1864995/148‑1 (MQ=255)
                                                                                                 aCGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGAt                                              >  1:2522163/1‑148 (MQ=255)
                                                                                                     tttCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGc                                          >  1:2249013/1‑148 (MQ=255)
                                                                                                       tCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAg                                        <  2:1383893/148‑1 (MQ=255)
                                                                                                       tCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAg                                        <  2:1383871/148‑1 (MQ=255)
                                                                                                             cAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGt                                  <  2:2842028/148‑1 (MQ=255)
                                                                                                             cAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGt                                  >  2:1394516/1‑148 (MQ=255)
                                                                                                             cAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGt                                  >  2:2316063/1‑148 (MQ=255)
                                                                                                              aaGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGtt                                 <  1:1312929/148‑1 (MQ=255)
                                                                                                              aaGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGtt                                 <  1:792747/148‑1 (MQ=255)
                                                                                                              aaGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGtt                                 >  2:1872331/1‑148 (MQ=255)
                                                                                                               aGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTg                                <  2:2512119/148‑1 (MQ=255)
                                                                                                                ggTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGt                               >  2:1059034/1‑148 (MQ=255)
                                                                                                                   aTCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCgg                            >  1:2059629/1‑148 (MQ=255)
                                                                                                                   aTCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCgg                            <  1:790079/148‑1 (MQ=255)
                                                                                                                     cGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCTCGAATGTCCAGCGGTAACGTCAGCGGCGTATCTCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCa                          <  2:2316261/148‑1 (MQ=255)
                                                                                                                     cGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCa                          <  2:2510714/148‑1 (MQ=255)
                                                                                                                            aGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGaa                   >  2:3092705/1‑148 (MQ=255)
                                                                                                                              tgcctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAAc                 >  2:2264798/1‑148 (MQ=255)
                                                                                                                              tgcctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAAc                 <  2:3430871/148‑1 (MQ=255)
                                                                                                                                cctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACAt               >  1:263797/1‑148 (MQ=255)
                                                                                                                                  tgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATct             <  2:692930/148‑1 (MQ=255)
                                                                                                                                    ccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATctgc           >  1:306013/1‑148 (MQ=255)
                                                                                                                                       gACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCtgctgc        >  1:2137179/1‑148 (MQ=255)
                                                                                                                                       gACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCtgctgc        >  1:2457828/1‑148 (MQ=255)
                                                                                                                                        aCTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCa       <  2:1972276/148‑1 (MQ=255)
                                                                                                                                         cTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCaa      <  1:877461/148‑1 (MQ=255)
                                                                                                                                          ttgttgCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAAc     >  1:1134583/1‑148 (MQ=255)
                                                                                                                                          ttgttgCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAAc     >  1:1134366/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCG  >  CP009273/2302925‑2303213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGAT  >  CP009273/2302923‑2303218
                                                                                                                                                   |                                                                                                                                                    
CAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGA                                                                                                                                                    >  SRR19973067.235036/1‑150 (MQ=60)
CAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGA                                                                                                                                                    >  SRR19973067.1301828/1‑150 (MQ=60)
  GGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAA                                                                                                                                                  >  SRR19973067.251684/1‑150 (MQ=60)
    GTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAG                                                                                                                                                <  SRR19973067.1829980/150‑1 (MQ=60)
    GTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAG                                                                                                                                                <  SRR19973067.1830312/150‑1 (MQ=60)
    GTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAG                                                                                                                                                <  SRR19973067.17354/150‑1 (MQ=60)
    GTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAG                                                                                                                                                <  SRR19973067.17620/150‑1 (MQ=60)
      ATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCA                                                                                                                                              >  SRR19973067.3311703/1‑150 (MQ=60)
      ATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCA                                                                                                                                              >  SRR19973067.2528286/1‑150 (MQ=60)
        TATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGT                                                                                                                                            <  SRR19973067.2419893/150‑1 (MQ=60)
         ATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTG                                                                                                                                           >  SRR19973067.3457654/1‑150 (MQ=60)
         ATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTG                                                                                                                                           >  SRR19973067.2528603/1‑150 (MQ=60)
         ATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTG                                                                                                                                           >  SRR19973067.1039403/1‑150 (MQ=60)
                  AGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATG                                                                                                                                  <  SRR19973067.3155890/150‑1 (MQ=60)
                  AGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATG                                                                                                                                  >  SRR19973067.3449823/1‑150 (MQ=60)
                  AGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATG                                                                                                                                  <  SRR19973067.1504036/150‑1 (MQ=60)
                    CTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTC                                                                                                                                >  SRR19973067.1304959/1‑150 (MQ=60)
                    CTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTC                                                                                                                                >  SRR19973067.697706/1‑150 (MQ=60)
                      TGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTACCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCA                                                                                                                              >  SRR19973067.1094021/1‑150 (MQ=60)
                       GTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAG                                                                                                                             <  SRR19973067.1080677/150‑1 (MQ=60)
                          GGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGG                                                                                                                          >  SRR19973067.1557126/1‑150 (MQ=60)
                           GCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGT                                                                                                                         <  SRR19973067.2645027/150‑1 (MQ=60)
                            CGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTA                                                                                                                        >  SRR19973067.997739/1‑150 (MQ=60)
                            CGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTA                                                                                                                        >  SRR19973067.1202539/1‑150 (MQ=60)
                             GGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAA                                                                                                                       >  SRR19973067.2435477/1‑150 (MQ=60)
                             GGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAA                                                                                                                       <  SRR19973067.3275408/150‑1 (MQ=60)
                               CACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACG                                                                                                                     >  SRR19973067.2408507/1‑150 (MQ=60)
                                ACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGT                                                                                                                    >  SRR19973067.2639468/1‑150 (MQ=60)
                                ACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGT                                                                                                                    >  SRR19973067.1953908/1‑150 (MQ=60)
                                 CAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTC                                                                                                                   >  SRR19973067.2949172/1‑150 (MQ=60)
                                   GGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAG                                                                                                                 >  SRR19973067.2303859/1‑150 (MQ=60)
                                   GGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAG                                                                                                                 >  SRR19973067.2654195/1‑150 (MQ=60)
                                   GGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAG                                                                                                                 >  SRR19973067.504843/1‑150 (MQ=60)
                                     CGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCG                                                                                                               >  SRR19973067.2481515/1‑150 (MQ=60)
                                           CAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAT                                                                                                         >  SRR19973067.203844/1‑150 (MQ=60)
                                           CAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAT                                                                                                         >  SRR19973067.3200592/1‑150 (MQ=60)
                                           CAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAT                                                                                                         <  SRR19973067.1886944/150‑1 (MQ=60)
                                           CAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAT                                                                                                         <  SRR19973067.79159/150‑1 (MQ=60)
                                             ACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCG                                                                                                       <  SRR19973067.2233959/150‑1 (MQ=60)
                                                  CCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTG                                                                                                  <  SRR19973067.1670428/150‑1 (MQ=60)
                                                    GTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGAG                                                                                                >  SRR19973067.1916204/1‑150 (MQ=60)
                                                      ACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGNGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTG                                                                                              <  SRR19973067.1815452/150‑1 (MQ=60)
                                                         GCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGAGTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGtgt                                                                                           <  SRR19973067.3531944/150‑4 (MQ=60)
                                                         GCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGG                                                                                           <  SRR19973067.3531934/150‑1 (MQ=60)
                                                         GCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGG                                                                                           <  SRR19973067.2072900/150‑1 (MQ=60)
                                                          CTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGC                                                                                          <  SRR19973067.1268623/150‑1 (MQ=60)
                                                               GGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCG                                                                                     <  SRR19973067.1869856/150‑1 (MQ=60)
                                                               GGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGAAGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCG                                                                                     <  SRR19973067.3490113/150‑1 (MQ=60)
                                                                   TGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCA                                                                                 <  SRR19973067.1381397/150‑1 (MQ=60)
                                                                   TGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCA                                                                                 <  SRR19973067.2641104/150‑1 (MQ=60)
                                                                       GATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTC                                                                             <  SRR19973067.1291111/150‑1 (MQ=60)
                                                                          GGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACG                                                                          >  SRR19973067.1429053/1‑150 (MQ=60)
                                                                          GGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACG                                                                          >  SRR19973067.1429000/1‑150 (MQ=60)
                                                                          GGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACG                                                                          <  SRR19973067.2021514/150‑1 (MQ=60)
                                                                               TAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACAT                                                                     <  SRR19973067.3385964/150‑1 (MQ=60)
                                                                               TAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACAT                                                                     >  SRR19973067.359112/1‑150 (MQ=60)
                                                                                AGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATG                                                                    <  SRR19973067.1881742/150‑1 (MQ=60)
                                                                                AGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATG                                                                    >  SRR19973067.267415/1‑150 (MQ=60)
                                                                                AGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATG                                                                    >  SRR19973067.2676032/1‑150 (MQ=60)
                                                                                          TGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAAC                                                          >  SRR19973067.2706874/1‑150 (MQ=60)
                                                                                              AACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCA                                                      >  SRR19973067.160027/1‑150 (MQ=60)
                                                                                               ACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAG                                                     <  SRR19973067.897825/150‑1 (MQ=60)
                                                                                               ACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAG                                                     <  SRR19973067.897821/150‑1 (MQ=60)
                                                                                               ACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAG                                                     <  SRR19973067.1877719/150‑1 (MQ=60)
                                                                                                 TGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGAT                                                   >  SRR19973067.2538698/1‑150 (MQ=60)
                                                                                                     GGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGC                                               >  SRR19973067.2263953/1‑150 (MQ=60)
                                                                                                      GTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCA                                              <  SRR19973067.429392/150‑1 (MQ=60)
                                                                                                      GTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCA                                              <  SRR19973067.2230987/150‑1 (MQ=60)
                                                                                                       TTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAG                                             >  SRR19973067.2255664/1‑150 (MQ=60)
                                                                                                             CGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGT                                       >  SRR19973067.2883380/1‑150 (MQ=60)
                                                                                                                AAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGT                                    >  SRR19973067.594633/1‑150 (MQ=60)
                                                                                                                AAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGT                                    <  SRR19973067.1321895/150‑1 (MQ=60)
                                                                                                                AAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGT                                    <  SRR19973067.798221/150‑1 (MQ=60)
                                                                                                                   GTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGG                                 >  SRR19973067.2073350/1‑150 (MQ=60)
                                                                                                                     ATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCA                               <  SRR19973067.795542/150‑1 (MQ=60)
                                                                                                                                TGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACAT                    >  SRR19973067.265588/1‑150 (MQ=60)
                                                                                                                                 GCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATC                   <  SRR19973067.2582720/150‑1 (MQ=60)
                                                                                                                                  CCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCT                  <  SRR19973067.867896/150‑1 (MQ=60)
                                                                                                                                    TGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGC                >  SRR19973067.307992/1‑150 (MQ=60)
                                                                                                                                    TGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGC                >  SRR19973067.307956/1‑150 (MQ=60)
                                                                                                                                       CAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGC             >  SRR19973067.2151461/1‑150 (MQ=60)
                                                                                                                                       CAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGC             >  SRR19973067.2473987/1‑150 (MQ=60)
                                                                                                                                          ACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAAC          >  SRR19973067.1142052/1‑150 (MQ=60)
                                                                                                                                          ACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAAC          >  SRR19973067.1142225/1‑150 (MQ=60)
                                                                                                                                          ACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAAC          >  SRR19973067.1142008/1‑150 (MQ=60)
                                                                                                                                           CTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACT         <  SRR19973067.883315/150‑1 (MQ=60)
                                                                                                                                                  CGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGAT  <  SRR19973067.2773204/150‑1 (MQ=60)
                                                                                                                                                  CGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGAT  <  SRR19973067.1447449/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTGAT  >  CP009273/2302923‑2303218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑