Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,825,702 C→A 100% K732N (AAG→AAT)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,825,7020CA99.4% 586.9 / ‑4.0 180K732N (AAG→AAT) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (93/85);  total (94/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 180 total aligned reads displayed.

CCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCT  >  CP009273/3825560‑3825846
                                                                                                                                              |                                                                                                                                                
 cGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcc                                                                                                                                            <  2:3087093/148‑1 (MQ=255)
 cGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcc                                                                                                                                            <  1:1717313/148‑1 (MQ=255)
   gCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcctc                                                                                                                                          >  2:2288637/1‑148 (MQ=255)
    cATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcctcg                                                                                                                                         >  2:3145158/1‑148 (MQ=255)
          aTCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCa                                                                                                                                   >  1:2416463/1‑148 (MQ=255)
          aTCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCa                                                                                                                                   >  2:241336/1‑148 (MQ=255)
           tCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAc                                                                                                                                  <  1:3414545/148‑1 (MQ=255)
            cAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAcc                                                                                                                                 >  1:3548769/1‑148 (MQ=255)
            cAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAcc                                                                                                                                 >  1:3548740/1‑148 (MQ=255)
              aTAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAg                                                                                                                               >  2:1073699/1‑148 (MQ=255)
                aGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTc                                                                                                                             >  1:749977/1‑148 (MQ=255)
                aGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTc                                                                                                                             >  1:1875768/1‑148 (MQ=255)
                   ttACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTcaca                                                                                                                          <  1:2666436/148‑1 (MQ=255)
                   ttACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTcaca                                                                                                                          >  2:132800/1‑148 (MQ=255)
                      cAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                       <  1:542588/148‑1 (MQ=255)
                      cAACGTAATTGTCAGCGCAGTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                       <  1:204252/148‑1 (MQ=255)
                           tAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgt                                                                                                                  >  2:2612241/1‑148 (MQ=255)
                           tAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgt                                                                                                                  >  2:2612169/1‑148 (MQ=255)
                            aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgtg                                                                                                                 <  1:2817213/148‑1 (MQ=255)
                            aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgtg                                                                                                                 <  1:3162660/148‑1 (MQ=255)
                              ttGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGtt                                                                                                               >  2:3022652/1‑148 (MQ=255)
                                 tCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGcc                                                                                                            >  1:3089525/1‑148 (MQ=255)
                                   aGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAg                                                                                                          >  1:3457341/1‑148 (MQ=255)
                                    gcgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGt                                                                                                         >  2:2003357/1‑148 (MQ=255)
                                    gcgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGt                                                                                                         >  2:3146212/1‑148 (MQ=255)
                                     cgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTa                                                                                                        >  1:3109326/1‑148 (MQ=255)
                                          tCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgc                                                                                                   <  2:2504101/148‑1 (MQ=255)
                                             cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctgc                                                                                                <  2:2711825/148‑1 (MQ=255)
                                             cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctgc                                                                                                <  1:3165166/148‑1 (MQ=255)
                                              ttGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTgctgct                                                                                               <  1:3477244/148‑1 (MQ=255)
                                                gAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCttt                                                                                             <  2:2725251/148‑1 (MQ=255)
                                                gAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCttt                                                                                             <  2:2883014/148‑1 (MQ=255)
                                                gAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCttt                                                                                             <  1:869155/148‑1 (MQ=255)
                                                 aGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCtttt                                                                                            >  2:1628337/1‑148 (MQ=255)
                                                 aGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCtttt                                                                                            >  2:1628695/1‑148 (MQ=255)
                                                  ggCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTta                                                                                           >  1:833624/1‑148 (MQ=255)
                                                  ggCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTta                                                                                           >  1:833623/1‑148 (MQ=255)
                                                    cTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaa                                                                                         <  2:251824/148‑1 (MQ=255)
                                                    cTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaa                                                                                         <  1:3234997/148‑1 (MQ=255)
                                                    cTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaa                                                                                         >  1:565069/1‑148 (MQ=255)
                                                     ttCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaag                                                                                        <  1:2560754/148‑1 (MQ=255)
                                                          caccaGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagtaaag                                                                                   >  1:312880/1‑148 (MQ=255)
                                                           accaGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGa                                                                                  <  2:793708/148‑1 (MQ=255)
                                                            ccaGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGAt                                                                                 >  2:463297/1‑148 (MQ=255)
                                                             caGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATc                                                                                <  2:1800938/148‑1 (MQ=255)
                                                             caGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATc                                                                                >  2:3328447/1‑148 (MQ=255)
                                                              aGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCa                                                                               <  1:1461445/148‑1 (MQ=255)
                                                              aGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCa                                                                               >  1:2074084/1‑148 (MQ=255)
                                                               gCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAc                                                                              <  2:2074084/148‑1 (MQ=255)
                                                               gCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAc                                                                              >  1:1867466/1‑148 (MQ=255)
                                                                 cccTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCg                                                                            >  1:1490758/1‑148 (MQ=255)
                                                                   cTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGAt                                                                          <  2:1461029/148‑1 (MQ=255)
                                                                     gCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATcc                                                                        <  2:1895053/148‑1 (MQ=255)
                                                                     gCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATcc                                                                        <  2:1895051/148‑1 (MQ=255)
                                                                       tCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGt                                                                      <  1:1997913/148‑1 (MQ=255)
                                                                       tCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGt                                                                      <  2:3548769/148‑1 (MQ=255)
                                                                       tCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGt                                                                      <  2:3548740/148‑1 (MQ=255)
                                                                          cTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagtaaag                                                                                   >  1:2481741/1‑132 (MQ=255)
                                                                          cTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcag                                                                   <  1:105195/148‑1 (MQ=255)
                                                                          cTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcag                                                                   <  2:2176599/148‑1 (MQ=255)
                                                                           tttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagc                                                                  >  2:1892872/1‑148 (MQ=255)
                                                                           tttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagc                                                                  <  1:531594/148‑1 (MQ=255)
                                                                           tttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagc                                                                  >  1:233444/1‑148 (MQ=255)
                                                                           tttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagc                                                                  <  1:268733/148‑1 (MQ=255)
                                                                             tCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagcag                                                                >  2:1261210/1‑148 (MQ=255)
                                                                              cAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCagcagc                                                               <  1:645156/148‑1 (MQ=255)
                                                                               aGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCg                                                              <  1:1208144/148‑1 (MQ=255)
                                                                                   tGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCa                                                          <  2:511985/148‑1 (MQ=255)
                                                                                   tGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCa                                                          <  1:2859424/148‑1 (MQ=255)
                                                                                   tGCGAACCGTCTTGCAGACCATTTACTTTCACAACAGTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCa                                                          <  2:3086195/148‑1 (MQ=255)
                                                                                     cGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACt                                                        >  2:2999164/1‑148 (MQ=255)
                                                                                        aCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTca                                                     >  1:1174454/1‑148 (MQ=255)
                                                                                            tCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGGGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGAACCGTCAGCAGCGGGCACTTCCCAGa                                                 >  2:1736512/1‑148 (MQ=255)
                                                                                             cTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAc                                                >  2:3147551/1‑148 (MQ=255)
                                                                                               tGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACgg                                              >  2:1372672/1‑148 (MQ=255)
                                                                                                gCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGc                                             <  1:1130424/148‑1 (MQ=255)
                                                                                                   gACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTc                                          >  1:3513063/1‑148 (MQ=255)
                                                                                                   gACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTc                                          <  2:3034082/148‑1 (MQ=255)
                                                                                                   gACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTc                                          >  2:1232281/1‑148 (MQ=255)
                                                                                                    aCCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCa                                         >  1:1077140/1‑148 (MQ=255)
                                                                                                    aCCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCa                                         >  2:2715869/1‑148 (MQ=255)
                                                                                                       aTTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGc                                      <  2:3457341/148‑1 (MQ=255)
                                                                                                       aTTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGc                                      <  2:1589462/148‑1 (MQ=255)
                                                                                                        tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCTCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGcc                                     <  2:3326888/148‑1 (MQ=255)
                                                                                                        tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGcc                                     >  2:277860/1‑148 (MQ=255)
                                                                                                         ttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGccc                                    <  1:1751358/148‑1 (MQ=255)
                                                                                                         ttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGccc                                    >  1:1703903/1‑148 (MQ=255)
                                                                                                           aCTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGt                                  >  1:423410/1‑148 (MQ=255)
                                                                                                           aCTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGt                                  >  1:3062383/1‑148 (MQ=255)
                                                                                                           aCTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGt                                  <  2:858386/148‑1 (MQ=255)
                                                                                                                       ttGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGa                      <  1:1979299/148‑1 (MQ=255)
                                                                                                                            cAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAgg                 >  2:2191043/1‑148 (MQ=255)
                                                                                                                               gCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTgg              <  2:410905/148‑1 (MQ=255)
                                                                                                                               gCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTgg              >  1:1097902/1‑148 (MQ=255)
                                                                                                                                cacaGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa             <  1:2181487/148‑1 (MQ=255)
                                                                                                                                cacaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa             <  1:3485372/148‑1 (MQ=255)
                                                                                                                                cacaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa             <  1:2050577/148‑1 (MQ=255)
                                                                                                                                cacaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa             <  1:132800/148‑1 (MQ=255)
                                                                                                                                cacaGTGTCCAGTTAGTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa             <  1:2181799/148‑1 (MQ=255)
                                                                                                                                    gtgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGc         >  1:1851251/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCT  >  CP009273/3825560‑3825846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTG  >  CP009273/3825555‑3825843
                                                                                                                                                   |                                                                                                                                             
CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATT                                                                                                                                             <  SRR19973067.1600168/150‑1 (MQ=60)
CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATT                                                                                                                                             <  SRR19973067.1600165/150‑1 (MQ=60)
CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATT                                                                                                                                             <  SRR19973067.1598731/150‑1 (MQ=60)
      CGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTC                                                                                                                                       <  SRR19973067.3499050/150‑1 (MQ=60)
      CGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTC                                                                                                                                       <  SRR19973067.1729082/150‑1 (MQ=60)
         CATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCC                                                                                                                                    >  SRR19973067.413766/1‑150 (MQ=60)
             CCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCA                                                                                                                                >  SRR19973067.2432294/1‑150 (MQ=60)
             CCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCA                                                                                                                                >  SRR19973067.27904/1‑150 (MQ=60)
               ATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACC                                                                                                                              >  SRR19973067.3571152/1‑150 (MQ=60)
               ATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACC                                                                                                                              >  SRR19973067.3571123/1‑150 (MQ=60)
               ATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACC                                                                                                                              >  SRR19973067.3571130/1‑150 (MQ=60)
                TCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCA                                                                                                                             <  SRR19973067.3436281/150‑1 (MQ=60)
                  AATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGT                                                                                                                           <  SRR19973067.614852/150‑1 (MQ=60)
                   ATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTC                                                                                                                          >  SRR19973067.755184/1‑150 (MQ=60)
                   ATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTC                                                                                                                          >  SRR19973067.1888567/1‑150 (MQ=60)
                        TTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGT                                                                                                                     <  SRR19973067.2683565/150‑1 (MQ=60)
                         TACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTA                                                                                                                    <  SRR19973067.2054491/150‑1 (MQ=60)
                           CAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAAT                                                                                                                  <  SRR19973067.546191/150‑1 (MQ=60)
                           CAACGTAATTGTCAGCGCAGTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAAT                                                                                                                  <  SRR19973067.205797/150‑1 (MQ=60)
                                TAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGT                                                                                                             <  SRR19973067.3111312/150‑1 (MQ=60)
                                 AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                            <  SRR19973067.3182692/150‑1 (MQ=60)
                                 AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                            <  SRR19973067.2835356/150‑1 (MQ=60)
                                 AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                            >  SRR19973067.3251436/1‑150 (MQ=60)
                                  ATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTG                                                                                                           <  SRR19973067.809058/150‑1 (MQ=60)
                                    TGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCC                                                                                                         >  SRR19973067.3109183/1‑150 (MQ=60)
                                      TCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAG                                                                                                       >  SRR19973067.3479325/1‑150 (MQ=60)
                                        AGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTA                                                                                                     >  SRR19973067.3129104/1‑150 (MQ=60)
                                        AGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTA                                                                                                     >  SRR19973067.1349844/1‑150 (MQ=60)
                                        AGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGACACGCCCGCACCCGACACAGTAATCGTGTTGCCAGTA                                                                                                     >  SRR19973067.3380256/1‑150 (MQ=60)
                                         GCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTAC                                                                                                    <  SRR19973067.2131690/150‑1 (MQ=60)
                                                CCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGC                                                                                             >  SRR19973067.2562598/1‑150 (MQ=60)
                                                  CTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTT                                                                                           <  SRR19973067.2491821/150‑1 (MQ=60)
                                                  CTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTT                                                                                           <  SRR19973067.3185210/150‑1 (MQ=60)
                                                   TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                          <  SRR19973067.3499327/150‑1 (MQ=60)
                                                   TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                          <  SRR19973067.3499322/150‑1 (MQ=60)
                                                     GAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTA                                                                                        >  SRR19973067.839295/1‑150 (MQ=60)
                                                     GAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTA                                                                                        >  SRR19973067.839296/1‑150 (MQ=60)
                                                     GAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTA                                                                                        <  SRR19973067.874965/150‑1 (MQ=60)
                                                      AGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGAGCTGCTTTTAA                                                                                       >  SRR19973067.864157/1‑150 (MQ=60)
                                                       GGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAA                                                                                      >  SRR19973067.568749/1‑150 (MQ=60)
                                                         CTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGT                                                                                    <  SRR19973067.3255289/150‑1 (MQ=60)
                                                          TTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTA                                                                                   <  SRR19973067.2577486/150‑1 (MQ=60)
                                                             ACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAG                                                                                >  SRR19973067.315795/1‑150 (MQ=60)
                                                             ACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAG                                                                                >  SRR19973067.314950/1‑150 (MQ=60)
                                                             ACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAG                                                                                <  SRR19973067.3041896/150‑1 (MQ=60)
                                                             ACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAG                                                                                >  SRR19973067.314948/1‑150 (MQ=60)
                                                             ACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAG                                                                                <  SRR19973067.2318971/150‑1 (MQ=60)
                                                               CACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGAT                                                                              <  SRR19973067.550359/150‑1 (MQ=60)
                                                                 CCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCA                                                                            >  SRR19973067.2087856/1‑150 (MQ=60)
                                                                  CAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAC                                                                           >  SRR19973067.1880207/1‑150 (MQ=60)
                                                                  CAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAC                                                                           >  SRR19973067.1880201/1‑150 (MQ=60)
                                                                   AGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACC                                                                          <  SRR19973067.1471206/150‑1 (MQ=60)
                                                                   AGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACC                                                                          <  SRR19973067.1108824/150‑1 (MQ=60)
                                                                    GCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCG                                                                         <  SRR19973067.28941/150‑1 (MQ=60)
                                                                    GCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCG                                                                         >  SRR19973067.1500620/1‑150 (MQ=60)
                                                                         TGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCG                                                                    <  SRR19973067.1477258/150‑1 (MQ=60)
                                                                            TCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCA                                                                 <  SRR19973067.2011400/150‑1 (MQ=60)
                                                                            TCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCA                                                                 <  SRR19973067.1523107/150‑1 (MQ=60)
                                                                             CACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATagatcggaagagca                                                                >  SRR19973067.2498058/1‑136 (MQ=60)
                                                                             CACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATagatcggaagagca                                                                >  SRR19973067.701748/1‑136 (MQ=60)
                                                                              ACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGC                                                               >  SRR19973067.235099/1‑150 (MQ=60)
                                                                               CTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCA                                                              <  SRR19973067.106238/150‑1 (MQ=60)
                                                                                TTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAG                                                             <  SRR19973067.270542/150‑1 (MQ=60)
                                                                                TTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAG                                                             <  SRR19973067.535158/150‑1 (MQ=60)
                                                                                   CAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGG                                                          <  SRR19973067.649519/150‑1 (MQ=60)
                                                                                    AGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGG                                                         <  SRR19973067.1216082/150‑1 (MQ=60)
                                                                                    AGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGG                                                         <  SRR19973067.1216035/150‑1 (MQ=60)
                                                                                        TGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACT                                                     <  SRR19973067.2877755/150‑1 (MQ=60)
                                                                                        TGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACT                                                     <  SRR19973067.1092418/150‑1 (MQ=60)
                                                                                          CGACCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGACCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTGTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTC                                                   >  SRR19973067.1914083/1‑150 (MQ=60)
                                                                                           GAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCA                                                  >  SRR19973067.1182230/1‑150 (MQ=60)
                                                                                           GAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCA                                                  >  SRR19973067.1181627/1‑150 (MQ=60)
                                                                                                GTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAC                                             <  SRR19973067.932911/150‑1 (MQ=60)
                                                                                                    TGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCT                                         <  SRR19973067.2068927/150‑1 (MQ=60)
                                                                                                     GCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTT                                        <  SRR19973067.1138052/150‑1 (MQ=60)
                                                                                                      CAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTC                                       >  SRR19973067.3535308/1‑150 (MQ=60)
                                                                                                      CAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTC                                       >  SRR19973067.3535647/1‑150 (MQ=60)
                                                                                                       AGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCA                                      >  SRR19973067.1084521/1‑150 (MQ=60)
                                                                                                           CATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCC                                  >  SRR19973067.2366570/1‑150 (MQ=60)
                                                                                                            ATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCC                                 >  SRR19973067.1715599/1‑150 (MQ=60)
                                                                                                            ATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCC                                 >  SRR19973067.1715634/1‑150 (MQ=60)
                                                                                                              TTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGT                               >  SRR19973067.3081875/1‑150 (MQ=60)
                                                                                                              TTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGT                               <  SRR19973067.1763298/150‑1 (MQ=60)
                                                                                                              TTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGT                               >  SRR19973067.426449/1‑150 (MQ=60)
                                                                                                              TTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGT                               >  SRR19973067.426354/1‑150 (MQ=60)
                                                                                                                      ACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGA                       <  SRR19973067.3459260/150‑1 (MQ=60)
                                                                                                                         ACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTG                    >  SRR19973067.2059971/1‑150 (MQ=60)
                                                                                                                          CATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGA                   >  SRR19973067.2695421/1‑150 (MQ=60)
                                                                                                                            TTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAG                 <  SRR19973067.1992703/150‑1 (MQ=60)
                                                                                                                            TTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAG                 <  SRR19973067.187172/150‑1 (MQ=60)
                                                                                                                                  AGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGG           >  SRR19973067.1105379/1‑150 (MQ=60)
                                                                                                                                     CACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAAT        <  SRR19973067.2196076/150‑1 (MQ=60)
                                                                                                                                     CACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAAT        <  SRR19973067.2064269/150‑1 (MQ=60)
                                                                                                                                     CACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAAT        <  SRR19973067.133996/150‑1 (MQ=60)
                                                                                                                                     CACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAAT        <  SRR19973067.3507483/150‑1 (MQ=60)
                                                                                                                                     CACAGTGTCCAGTTAGTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAAT        <  SRR19973067.2196389/150‑1 (MQ=60)
                                                                                                                                       CAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGC      >  SRR19973067.1863870/1‑150 (MQ=60)
                                                                                                                                           GTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTG  <  SRR19973067.176756/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                             
CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTG  >  CP009273/3825555‑3825843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑