Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,825,702 C→A 100% K732N (AAG→AAT)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,825,7020CA99.3% 954.4 / ‑1.7 271K732N (AAG→AAT) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (143/125);  total (145/126)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC  >  CP009273/3825555‑3825848
                                                                                                                                                   |                                                                                                                                                  
cGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGttatt                                                                                                                                                  <  2:79024/150‑1 (MQ=255)
cGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGttatt                                                                                                                                                  >  2:1016400/1‑150 (MQ=255)
 gTACCCGGCATTCCCTCAAGAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGttcttc                                                                                                                                                 <  2:751958/150‑1 (MQ=255)
 gTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGttattc                                                                                                                                                 >  1:424986/1‑150 (MQ=255)
     ccGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcct                                                                                                                                             <  1:3772958/150‑1 (MQ=255)
     ccGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcct                                                                                                                                             >  2:3415345/1‑150 (MQ=255)
     ccGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCAGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTAACTTTAACAACATTGCGCAGGCACAGAGTCCAGTTATtcgcct                                                                                                                                             >  2:914448/1‑150 (MQ=255)
      cGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcctc                                                                                                                                            <  2:401348/150‑1 (MQ=255)
      cGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcctc                                                                                                                                            >  2:4074820/1‑150 (MQ=255)
          aTTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGccc                                                                                                                                        <  2:1918353/150‑1 (MQ=255)
                 cAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAg                                                                                                                                 <  1:3961802/150‑1 (MQ=255)
                  aaTAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGt                                                                                                                                <  2:3077516/150‑1 (MQ=255)
                      gATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTcaca                                                                                                                            <  1:3987926/150‑1 (MQ=255)
                        ttACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGt                                                                                                                          <  1:2137719/150‑1 (MQ=255)
                        ttACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGt                                                                                                                          <  2:1167955/150‑1 (MQ=255)
                         tACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACCTTGCGCAGGCACAGTGTCCAGTTAGTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                         <  2:7179/150‑1 (MQ=255)
                         tACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                         <  1:1576211/150‑1 (MQ=255)
                          aCAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTaa                                                                                                                        <  2:2829465/150‑1 (MQ=255)
                            aaCGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATc                                                                                                                      >  2:1903524/1‑150 (MQ=255)
                             aCGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCg                                                                                                                     <  2:3507393/150‑1 (MQ=255)
                                tAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACACCATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgtgt                                                                                                                  <  2:1724966/150‑1 (MQ=255)
                                tAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgtgt                                                                                                                  <  2:1440533/150‑1 (MQ=255)
                                 aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGtt                                                                                                                 <  2:2222088/150‑1 (MQ=255)
                                 aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGtt                                                                                                                 >  2:1961868/1‑150 (MQ=255)
                                 aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGtt                                                                                                                 >  1:2725709/1‑150 (MQ=255)
                                  aTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTg                                                                                                                <  2:3175401/150‑1 (MQ=255)
                                  aTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTg                                                                                                                <  1:1262806/150‑1 (MQ=255)
                                    tGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGcc                                                                                                              >  2:957547/1‑150 (MQ=255)
                                    tGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGcc                                                                                                              >  1:1449787/1‑150 (MQ=255)
                                     gTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCa                                                                                                             <  2:2776827/150‑1 (MQ=255)
                                        aGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTa                                                                                                          >  2:2008004/1‑150 (MQ=255)
                                        aGCGCATTCCCTTGAGGCTACACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTa                                                                                                          <  2:1003453/150‑1 (MQ=255)
                                         gcgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTAc                                                                                                         <  2:425742/150‑1 (MQ=255)
                                          cgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCGGTACg                                                                                                        >  2:380054/1‑150 (MQ=255)
                                           gcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGt                                                                                                       >  2:3443075/1‑150 (MQ=255)
                                               tCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctg                                                                                                   >  2:3982883/1‑150 (MQ=255)
                                                 ccTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTgctgct                                                                                                 >  1:1643401/1‑150 (MQ=255)
                                                 ccTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTgctgct                                                                                                 >  1:2886288/1‑150 (MQ=255)
                                                 ccTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTgctgct                                                                                                 >  1:4079705/1‑150 (MQ=255)
                                                  cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCtt                                                                                                <  1:831138/150‑1 (MQ=255)
                                                   ttGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCttt                                                                                               <  1:4015213/150‑1 (MQ=255)
                                                      aGGCTTCACCACCAGCCCCTGCTCCCTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACCTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCAAAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaa                                                                                            <  1:2323879/150‑1 (MQ=255)
                                                           tCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagtaa                                                                                       <  1:2947546/150‑1 (MQ=255)
                                                                   aGCCCCTTCTCACTTTCAGACTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGTCACAGTGTCCAGTTATTCGCCTCGCCCGCACAATTCACATTATTCGTGTTGCCATTACTTGCTGCTTTTAAAGTAAAGATCAcc                                                                               <  1:4912/150‑1 (MQ=255)
                                                                   aGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAcc                                                                               >  1:2213976/1‑150 (MQ=255)
                                                                    gCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCg                                                                              <  2:505759/150‑1 (MQ=255)
                                                                      cccTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGAt                                                                            >  2:2501912/1‑150 (MQ=255)
                                                                           cTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTc                                                                       <  1:2874190/150‑1 (MQ=255)
                                                                            tCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTca                                                                      <  1:670188/150‑1 (MQ=255)
                                                                             cACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcag                                                                     >  1:3418491/1‑150 (MQ=255)
                                                                               cTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCTCAGTAATCGTGTTGACATTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagca                                                                   <  1:2233778/150‑1 (MQ=255)
                                                                                tttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagcag                                                                  <  1:567278/150‑1 (MQ=255)
                                                                                 ttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCagcagc                                                                 <  2:3931779/150‑1 (MQ=255)
                                                                                    aGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACCTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCggg                                                              <  2:62049/150‑1 (MQ=255)
                                                                                    aGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCggg                                                              <  2:3335047/150‑1 (MQ=255)
                                                                                    aGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCggg                                                              >  1:2629914/1‑150 (MQ=255)
                                                                                      ccTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCa                                                            >  1:2379006/1‑150 (MQ=255)
                                                                                       cTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCAc                                                           >  2:1358059/1‑150 (MQ=255)
                                                                                            aaCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTcac                                                      <  2:3375585/150‑1 (MQ=255)
                                                                                             aCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTcaca                                                     >  2:1575943/1‑150 (MQ=255)
                                                                                              ccGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAg                                                    >  2:86418/1‑150 (MQ=255)
                                                                                                gTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAc                                                  <  1:4090820/150‑1 (MQ=255)
                                                                                                 tCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACg                                                 <  2:2655628/150‑1 (MQ=255)
                                                                                                  cTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACgg                                                <  2:482576/150‑1 (MQ=255)
                                                                                                    tGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCt                                              <  1:2434031/150‑1 (MQ=255)
                                                                                                    tGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCt                                              >  1:3094081/1‑150 (MQ=255)
                                                                                                     gCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCtt                                             <  1:894231/150‑1 (MQ=255)
                                                                                                      cAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTc                                            <  1:2734368/150‑1 (MQ=255)
                                                                                                       aGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCa                                           <  2:3748663/150‑1 (MQ=255)
                                                                                                       aGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCa                                           <  2:1494835/150‑1 (MQ=255)
                                                                                                         aCCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATg                                         >  1:3984960/1‑150 (MQ=255)
                                                                                                             tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCg                                     >  1:1890098/1‑150 (MQ=255)
                                                                                                             tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCg                                     >  1:1890351/1‑150 (MQ=255)
                                                                                                               tACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTc                                   >  1:1172454/1‑150 (MQ=255)
                                                                                                                 cTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCtt                                 >  2:166584/1‑150 (MQ=255)
                                                                                                                 cTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCtt                                 <  1:774776/150‑1 (MQ=255)
                                                                                                                     cacaACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAg                             >  1:2718101/1‑150 (MQ=255)
                                                                                                                       caacaTTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAt                           >  2:4096931/1‑150 (MQ=255)
                                                                                                                        aacaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAtt                          >  2:3614534/1‑150 (MQ=255)
                                                                                                                        aacaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAtt                          <  1:4037517/150‑1 (MQ=255)
                                                                                                                        aacaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAtt                          >  1:1785140/1‑150 (MQ=255)
                                                                                                                          caTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGa                        >  2:2325424/1‑150 (MQ=255)
                                                                                                                           aTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACATACGGCTTCATGCCCGTCTTGCAGATTGaa                       >  2:2120131/1‑150 (MQ=255)
                                                                                                                             tGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAaga                     <  2:2423543/150‑1 (MQ=255)
                                                                                                                                gcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGt                  <  2:799693/150‑1 (MQ=255)
                                                                                                                                 cAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTg                 <  1:2021814/150‑1 (MQ=255)
                                                                                                                                  aGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTgg                >  2:129320/1‑150 (MQ=255)
                                                                                                                                  aGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTgg                >  1:3121394/1‑150 (MQ=255)
                                                                                                                                  aGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTgg                >  2:3181888/1‑150 (MQ=255)
                                                                                                                                    gCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGaa              <  1:1346892/150‑1 (MQ=255)
                                                                                                                                     cacaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAAt             <  2:1777531/150‑1 (MQ=255)
                                                                                                                                        aGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGcc          >  1:1005821/1‑150 (MQ=255)
                                                                                                                                        aGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGcc          >  1:1005813/1‑150 (MQ=255)
                                                                                                                                        aGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGcc          >  2:1070046/1‑150 (MQ=255)
                                                                                                                                         gtgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCg         >  2:314023/1‑150 (MQ=255)
                                                                                                                                          tgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGt        >  1:3174389/1‑150 (MQ=255)
                                                                                                                                           gtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTg       <  2:631983/150‑1 (MQ=255)
                                                                                                                                           gtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTg       <  2:2039228/150‑1 (MQ=255)
                                                                                                                                           gtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGGTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTg       <  2:2717159/150‑1 (MQ=255)
                                                                                                                                                gTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTc  >  2:942332/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC  >  CP009273/3825555‑3825848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGT  >  CP009273/3825553‑3825850
                                                                                                                                                     |                                                                                                                                                    
GACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTA                                                                                                                                                      <  SRR19973074.2068867/150‑1 (MQ=60)
  CGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATT                                                                                                                                                    >  SRR19973074.1246794/1‑150 (MQ=60)
   GTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTC                                                                                                                                                   >  SRR19973074.425808/1‑150 (MQ=60)
    TACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCG                                                                                                                                                  >  SRR19973074.2043095/1‑150 (MQ=60)
    TACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTATCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCG                                                                                                                                                  >  SRR19973074.2043071/1‑150 (MQ=60)
     ACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGC                                                                                                                                                 <  SRR19973074.2332051/150‑1 (MQ=60)
      CCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCC                                                                                                                                                >  SRR19973074.1518210/1‑150 (MQ=60)
      CCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCC                                                                                                                                                >  SRR19973074.1908771/1‑150 (MQ=60)
       CCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCT                                                                                                                                               <  SRR19973074.2727409/150‑1 (MQ=60)
       CCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCT                                                                                                                                               <  SRR19973074.3779883/150‑1 (MQ=60)
         GGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCG                                                                                                                                             >  SRR19973074.409808/1‑150 (MQ=60)
              TCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGC                                                                                                                                        >  SRR19973074.3987483/1‑150 (MQ=60)
                CATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAC                                                                                                                                      >  SRR19973074.2732301/1‑150 (MQ=60)
                CATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAC                                                                                                                                      >  SRR19973074.4089919/1‑150 (MQ=60)
                   CAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAG                                                                                                                                   <  SRR19973074.3969102/150‑1 (MQ=60)
                        GATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACA                                                                                                                              <  SRR19973074.3995289/150‑1 (MQ=60)
                        GATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACA                                                                                                                              <  SRR19973074.3995267/150‑1 (MQ=60)
                         ATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAG                                                                                                                             >  SRR19973074.874323/1‑150 (MQ=60)
                          TTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGT                                                                                                                            <  SRR19973074.2141761/150‑1 (MQ=60)
                          TTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGT                                                                                                                            <  SRR19973074.2141732/150‑1 (MQ=60)
                           TACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTA                                                                                                                           <  SRR19973074.1579208/150‑1 (MQ=60)
                                   AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                   >  SRR19973074.2730746/1‑150 (MQ=60)
                                   AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                   <  SRR19973074.827573/150‑1 (MQ=60)
                                   AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                   <  SRR19973074.1562648/150‑1 (MQ=60)
                                    ATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTG                                                                                                                  <  SRR19973074.1265230/150‑1 (MQ=60)
                                      TGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCC                                                                                                                >  SRR19973074.1452498/1‑150 (MQ=60)
                                      TGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCC                                                                                                                >  SRR19973074.111315/1‑150 (MQ=60)
                                      TGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCC                                                                                                                >  SRR19973074.653950/1‑150 (MQ=60)
                                       GTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCG                                                                                                               <  SRR19973074.2732261/150‑1 (MQ=60)
                                              CATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTG                                                                                                        >  SRR19973074.2186277/1‑150 (MQ=60)
                                                TTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCT                                                                                                      <  SRR19973074.2728363/150‑1 (MQ=60)
                                                TTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCAAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCT                                                                                                      >  SRR19973074.2929417/1‑150 (MQ=60)
                                                 TCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTG                                                                                                     >  SRR19973074.4036359/1‑150 (MQ=60)
                                                  CCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGC                                                                                                    >  SRR19973074.573359/1‑150 (MQ=60)
                                                   CCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCT                                                                                                   >  SRR19973074.2891642/1‑150 (MQ=60)
                                                   CCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCT                                                                                                   >  SRR19973074.4087254/1‑150 (MQ=60)
                                                   CCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCT                                                                                                   <  SRR19973074.2308844/150‑1 (MQ=60)
                                                   CCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCT                                                                                                   <  SRR19973074.2308851/150‑1 (MQ=60)
                                                   CCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCT                                                                                                   >  SRR19973074.4087073/1‑150 (MQ=60)
                                                   CCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCT                                                                                                   >  SRR19973074.1646510/1‑150 (MQ=60)
                                                    CTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTT                                                                                                  <  SRR19973074.832735/150‑1 (MQ=60)
                                                     TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                                 <  SRR19973074.4022611/150‑1 (MQ=60)
                                                     TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                                 <  SRR19973074.4022638/150‑1 (MQ=60)
                                                     TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                                 <  SRR19973074.1774471/150‑1 (MQ=60)
                                                      TGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTT                                                                                                >  SRR19973074.2952195/1‑150 (MQ=60)
                                                       GAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTA                                                                                               >  SRR19973074.52028/1‑150 (MQ=60)
                                                        AGGCTTCACCACCAGCCCCTGCTCCCTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACCTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCAAAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAA                                                                                              <  SRR19973074.2328218/150‑1 (MQ=60)
                                                        AGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAA                                                                                              <  SRR19973074.2328220/150‑1 (MQ=60)
                                                        AGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAA                                                                                              >  SRR19973074.950003/1‑150 (MQ=60)
                                                          GCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAG                                                                                            >  SRR19973074.2773495/1‑150 (MQ=60)
                                                           CTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGT                                                                                           <  SRR19973074.4157691/150‑1 (MQ=60)
                                                           CTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGT                                                                                           >  SRR19973074.3634658/1‑150 (MQ=60)
                                                             TCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAA                                                                                         <  SRR19973074.2953025/150‑1 (MQ=60)
                                                                  ACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATC                                                                                    <  SRR19973074.4062647/150‑1 (MQ=60)
                                                                   CCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCA                                                                                   <  SRR19973074.562518/150‑1 (MQ=60)
                                                                     AGCCCCTTCTCACTTTCAGACTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGTCACAGTGTCCAGTTATTCGCCTCGCCCGCACAATTCACATTATTCGTGTTGCCATTACTTGCTGCTTTTAAAGTAAAGATCACC                                                                                 <  SRR19973074.4922/150‑1 (MQ=60)
                                                                     AGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACC                                                                                 >  SRR19973074.2619032/1‑150 (MQ=60)
                                                                     AGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACC                                                                                 >  SRR19973074.2218145/1‑150 (MQ=60)
                                                                     AGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACC                                                                                 >  SRR19973074.2218205/1‑150 (MQ=60)
                                                                      GCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCG                                                                                >  SRR19973074.3920955/1‑150 (MQ=60)
                                                                       CCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGA                                                                               >  SRR19973074.1295532/1‑150 (MQ=60)
                                                                            GCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGT                                                                          >  SRR19973074.4030821/1‑150 (MQ=60)
                                                                             CTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTC                                                                         <  SRR19973074.2879543/150‑1 (MQ=60)
                                                                             CTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTC                                                                         <  SRR19973074.2879524/150‑1 (MQ=60)
                                                                              TCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCA                                                                        <  SRR19973074.671423/150‑1 (MQ=60)
                                                                               CACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAG                                                                       >  SRR19973074.3424800/1‑150 (MQ=60)
                                                                               CACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAG                                                                       >  SRR19973074.3424812/1‑150 (MQ=60)
                                                                                 CTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCTCAGTAATCGTGTTGACATTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCA                                                                     <  SRR19973074.2237977/150‑1 (MQ=60)
                                                                                 CTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCA                                                                     <  SRR19973074.466418/150‑1 (MQ=60)
                                                                                  TTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAG                                                                    <  SRR19973074.568392/150‑1 (MQ=60)
                                                                                      CGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCTCCGATCCGTCAGCAGCGGG                                                                <  SRR19973074.3272793/150‑1 (MQ=60)
                                                                                      AGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGG                                                                >  SRR19973074.2634832/1‑150 (MQ=60)
                                                                                       GCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGC                                                               <  SRR19973074.2391623/150‑1 (MQ=60)
                                                                                        CCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCA                                                              >  SRR19973074.2551621/1‑150 (MQ=60)
                                                                                        CCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCA                                                              >  SRR19973074.2383429/1‑150 (MQ=60)
                                                                                              AACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCAC                                                        >  SRR19973074.1903599/1‑150 (MQ=60)
                                                                                                  GTCTTGCAGACCATTTACTTTCACACCATTGCGCAGGCACAGTGTCCAGTTTTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCTCTTCACAGAC                                                    <  SRR19973074.2424510/150‑1 (MQ=60)
                                                                                                  GTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAC                                                    <  SRR19973074.3135849/150‑1 (MQ=60)
                                                                                                  GTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAC                                                    <  SRR19973074.4098383/150‑1 (MQ=60)
                                                                                                   TCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACG                                                   <  SRR19973074.3991342/150‑1 (MQ=60)
                                                                                                      TGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCT                                                >  SRR19973074.3099764/1‑150 (MQ=60)
                                                                                                      TGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCT                                                <  SRR19973074.1615614/150‑1 (MQ=60)
                                                                                                      TGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCT                                                <  SRR19973074.2438534/150‑1 (MQ=60)
                                                                                                      TGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCT                                                >  SRR19973074.3099783/1‑150 (MQ=60)
                                                                                                       GCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTT                                               <  SRR19973074.895938/150‑1 (MQ=60)
                                                                                                        CAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTC                                              <  SRR19973074.2739415/150‑1 (MQ=60)
                                                                                                         AGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCA                                             <  SRR19973074.907800/150‑1 (MQ=60)
                                                                                                         AGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCA                                             <  SRR19973074.1796356/150‑1 (MQ=60)
                                                                                                         AGACCATTTACTTACACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAAGGCTTCA                                             >  SRR19973074.1346102/1‑150 (MQ=60)
                                                                                                          GACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCAT                                            >  SRR19973074.2957935/1‑150 (MQ=60)
                                                                                                          GACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCAT                                            >  SRR19973074.2957944/1‑150 (MQ=60)
                                                                                                           ACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATG                                           <  SRR19973074.1100066/150‑1 (MQ=60)
                                                                                                           ACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATG                                           >  SRR19973074.3992311/1‑150 (MQ=60)
                                                                                                           ACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATG                                           >  SRR19973074.3996298/1‑150 (MQ=60)
                                                                                                           ACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATG                                           >  SRR19973074.1892671/1‑150 (MQ=60)
                                                                                                               TTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCG                                       >  SRR19973074.1893971/1‑150 (MQ=60)
                                                                                                               TTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCG                                       >  SRR19973074.1893718/1‑150 (MQ=60)
                                                                                                               TTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCG                                       >  SRR19973074.1893933/1‑150 (MQ=60)
                                                                                                                 TACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTC                                     >  SRR19973074.1174684/1‑150 (MQ=60)
                                                                                                                   CTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTT                                   <  SRR19973074.776877/150‑1 (MQ=60)
                                                                                                                   CTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTT                                   <  SRR19973074.776262/150‑1 (MQ=60)
                                                                                                                   CTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTT                                   <  SRR19973074.466324/150‑1 (MQ=60)
                                                                                                                       CACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAG                               <  SRR19973074.1769678/150‑1 (MQ=60)
                                                                                                                       CACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAG                               >  SRR19973074.2723129/1‑150 (MQ=60)
                                                                                                                         CAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAT                             <  SRR19973074.1606983/150‑1 (MQ=60)
                                                                                                                          AACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATT                            <  SRR19973074.4044979/150‑1 (MQ=60)
                                                                                                                          AACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATT                            >  SRR19973074.1788521/1‑150 (MQ=60)
                                                                                                                            CATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGAGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGA                          >  SRR19973074.67419/1‑150 (MQ=60)
                                                                                                                                   CAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTG                   <  SRR19973074.2025638/150‑1 (MQ=60)
                                                                                                                                    AGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGG                  >  SRR19973074.3127118/1‑150 (MQ=60)
                                                                                                                                     GGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGA                 >  SRR19973074.3801298/1‑150 (MQ=60)
                                                                                                                                      GCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAA                <  SRR19973074.1349402/150‑1 (MQ=60)
                                                                                                                                      GCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAA                <  SRR19973074.1349823/150‑1 (MQ=60)
                                                                                                                                        ACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATG              >  SRR19973074.2829695/1‑150 (MQ=60)
                                                                                                                                          AGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCC            >  SRR19973074.1007680/1‑150 (MQ=60)
                                                                                                                                          AGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCC            >  SRR19973074.1007688/1‑150 (MQ=60)
                                                                                                                                          AGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCC            >  SRR19973074.1007685/1‑150 (MQ=60)
                                                                                                                                            TGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGT          >  SRR19973074.1220062/1‑150 (MQ=60)
                                                                                                                                            TGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGT          >  SRR19973074.3180231/1‑150 (MQ=60)
                                                                                                                                            TGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGT          >  SRR19973074.2686201/1‑150 (MQ=60)
                                                                                                                                            TGTCCAGTTATTCGCCACGCCCGCACCAGTAACAGTAATCGTGTTGCCAGAACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGT          >  SRR19973074.3180155/1‑150 (MQ=60)
                                                                                                                                             GTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTG         >  SRR19973074.3114815/1‑150 (MQ=60)
                                                                                                                                             GTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTG         <  SRR19973074.879278/150‑1 (MQ=60)
                                                                                                                                                    TATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGT  <  SRR19973074.2463802/150‑1 (MQ=60)
                                                                                                                                                    TATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGT  <  SRR19973074.2463786/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGT  >  CP009273/3825553‑3825850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑