Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,825,702 C→A 100% K732N (AAG→AAT)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,825,7020CA99.5% 628.8 / ‑4.2 189K732N (AAG→AAT) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (100/88);  total (100/89)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

TACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCC  >  CP009273/3825557‑3825845
                                                                                                                                                 |                                                                                                                                               
gaCCCTGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGAGAACCGTCTTGCAGACCATTTACTTTCACAACCGTGCGCAGGCACAGTGTCCAGttatt                                                                                                                                               <  2:1998789/147‑1 (MQ=255)
 aCCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGttattc                                                                                                                                              >  1:3044576/1‑148 (MQ=255)
 aCCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGttattc                                                                                                                                              >  1:1806382/1‑148 (MQ=255)
   ccGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgc                                                                                                                                            <  2:3514161/148‑1 (MQ=255)
    cGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcc                                                                                                                                           <  1:598331/148‑1 (MQ=255)
         ttCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcctcgcc                                                                                                                                      >  2:1734908/1‑148 (MQ=255)
          tCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGccc                                                                                                                                     >  2:513040/1‑148 (MQ=255)
             aTCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCa                                                                                                                                  >  1:3390156/1‑148 (MQ=255)
              tCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAc                                                                                                                                 >  1:1800150/1‑148 (MQ=255)
               cAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAcc                                                                                                                                >  1:2080092/1‑148 (MQ=255)
                aaTAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCa                                                                                                                               <  1:2625271/148‑1 (MQ=255)
                aaTAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCa                                                                                                                               <  1:3506393/148‑1 (MQ=255)
                  tAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGt                                                                                                                             <  2:2693418/148‑1 (MQ=255)
                   aGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTc                                                                                                                            >  1:3784647/1‑148 (MQ=255)
                      ttACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTcaca                                                                                                                         >  2:1276322/1‑148 (MQ=255)
                       tACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAg                                                                                                                        >  2:1104119/1‑148 (MQ=255)
                         cAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                      <  2:3159473/148‑1 (MQ=255)
                         cAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                      <  2:2039998/148‑1 (MQ=255)
                            cGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATc                                                                                                                   >  2:3336966/1‑148 (MQ=255)
                               aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACACCAGTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgtg                                                                                                                <  1:2127429/148‑1 (MQ=255)
                               aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgtg                                                                                                                <  1:1885343/148‑1 (MQ=255)
                                aTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgtgt                                                                                                               >  1:1625031/1‑148 (MQ=255)
                                 ttGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGtt                                                                                                              >  2:782249/1‑148 (MQ=255)
                                 ttGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGtt                                                                                                              >  2:2326251/1‑148 (MQ=255)
                                   gTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGc                                                                                                            <  1:2073458/148‑1 (MQ=255)
                                       gcgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTTCAGACCATTTAATTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGt                                                                                                        <  2:1267754/148‑1 (MQ=255)
                                       gcgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGt                                                                                                        <  2:1270615/148‑1 (MQ=255)
                                          cATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACg                                                                                                     <  1:3380458/148‑1 (MQ=255)
                                            ttCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtg                                                                                                   <  1:3101018/148‑1 (MQ=255)
                                             tCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgc                                                                                                  <  2:1889828/148‑1 (MQ=255)
                                               ccTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCCCAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTACTCGTGTTGCCAGTCCGtgctg                                                                                                >  2:2576330/1‑148 (MQ=255)
                                                cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACCTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATAGTGTTGCCAGTACGtgctgc                                                                                               <  1:3345980/148‑1 (MQ=255)
                                                cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctgc                                                                                               >  1:1734508/1‑148 (MQ=255)
                                                cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctgc                                                                                               >  1:185163/1‑148 (MQ=255)
                                                cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctgc                                                                                               <  1:560408/148‑1 (MQ=255)
                                                cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctgc                                                                                               <  2:2910595/148‑1 (MQ=255)
                                                cTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgctgc                                                                                               >  1:3118943/1‑148 (MQ=255)
                                                 ttGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTgctgct                                                                                              <  1:1588209/148‑1 (MQ=255)
                                                  tGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCtt                                                                                             <  2:3416496/148‑1 (MQ=255)
                                                     ggCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTta                                                                                          >  1:908790/1‑148 (MQ=255)
                                                      gCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaa                                                                                         <  2:3294155/148‑1 (MQ=255)
                                                         tCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTGCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagt                                                                                      >  1:1988996/1‑148 (MQ=255)
                                                               ccaGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGAt                                                                                >  1:2932612/1‑148 (MQ=255)
                                                               ccaGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGAt                                                                                >  1:2932506/1‑148 (MQ=255)
                                                                caGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATc                                                                               <  1:219604/148‑1 (MQ=255)
                                                                  gCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAc                                                                             >  1:2058489/1‑148 (MQ=255)
                                                                       tGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATc                                                                        >  1:3518373/1‑148 (MQ=255)
                                                                               ttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGTTCCGTcagca                                                                >  2:1068768/1‑148 (MQ=255)
                                                                               ttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGTTCCGTcagca                                                                >  2:1068738/1‑148 (MQ=255)
                                                                               ttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagca                                                                <  2:1665419/148‑1 (MQ=255)
                                                                               ttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcagca                                                                <  1:3186584/148‑1 (MQ=255)
                                                                                 cAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCagcagc                                                              >  2:1443994/1‑148 (MQ=255)
                                                                                       gCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCAc                                                        <  2:238769/148‑1 (MQ=255)
                                                                                          aaCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTc                                                     >  2:857106/1‑148 (MQ=255)
                                                                                          aaCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTc                                                     >  2:1965781/1‑148 (MQ=255)
                                                                                                cTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAc                                               >  1:640634/1‑148 (MQ=255)
                                                                                                 ttGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACg                                              >  2:2442814/1‑148 (MQ=255)
                                                                                                  tGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACgg                                             <  2:288918/148‑1 (MQ=255)
                                                                                                   gCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGc                                            <  2:956639/148‑1 (MQ=255)
                                                                                                   gCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGc                                            <  2:1117959/148‑1 (MQ=255)
                                                                                                   gCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGc                                            <  1:513040/148‑1 (MQ=255)
                                                                                                   gCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGc                                            <  1:2294036/148‑1 (MQ=255)
                                                                                                    cAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCATAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCt                                           <  1:2221246/148‑1 (MQ=255)
                                                                                                     aGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCtt                                          <  2:3654184/148‑1 (MQ=255)
                                                                                                     aGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCtt                                          >  1:345747/1‑148 (MQ=255)
                                                                                                       aCCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCa                                        >  2:2773934/1‑148 (MQ=255)
                                                                                                        ccATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCAt                                       >  2:920705/1‑148 (MQ=255)
                                                                                                         cATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATg                                      <  1:2993538/148‑1 (MQ=255)
                                                                                                         cATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATg                                      >  2:3357302/1‑148 (MQ=255)
                                                                                                         cATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATg                                      >  1:1896464/1‑148 (MQ=255)
                                                                                                         cATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATg                                      >  1:1169773/1‑148 (MQ=255)
                                                                                                         cATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATg                                      <  1:1088112/148‑1 (MQ=255)
                                                                                                           tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGcc                                    <  2:3067640/148‑1 (MQ=255)
                                                                                                           tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGcc                                    <  2:3068491/148‑1 (MQ=255)
                                                                                                           tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGcc                                    >  1:2537467/1‑148 (MQ=255)
                                                                                                           tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGcc                                    <  2:1600785/148‑1 (MQ=255)
                                                                                                           tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGcc                                    >  1:1987891/1‑148 (MQ=255)
                                                                                                              aCTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGt                                 <  1:3611366/148‑1 (MQ=255)
                                                                                                              aCTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGt                                 <  1:3693744/148‑1 (MQ=255)
                                                                                                              aCTTTCACAACAGTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGt                                 <  2:2943154/148‑1 (MQ=255)
                                                                                                                tttCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCt                               >  1:895694/1‑148 (MQ=255)
                                                                                                                  tCACAACAGTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCAGGCCCGTCTTg                             <  2:1374271/148‑1 (MQ=255)
                                                                                                                   cacaACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGc                            <  1:1443994/148‑1 (MQ=255)
                                                                                                                       acaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAt                        <  1:1527884/148‑1 (MQ=255)
                                                                                                                       acaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAt                        >  1:712269/1‑148 (MQ=255)
                                                                                                                          ttGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGa                     >  1:2375443/1‑148 (MQ=255)
                                                                                                                            gcgcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAag                   <  1:3675048/148‑1 (MQ=255)
                                                                                                                             cgcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAaga                  <  2:2224561/148‑1 (MQ=255)
                                                                                                                                  gCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTgg             >  1:2568249/1‑148 (MQ=255)
                                                                                                                                   cacaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa            >  2:1312620/1‑148 (MQ=255)
                                                                                                                                   cacaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa            >  2:458766/1‑148 (MQ=255)
                                                                                                                                   cacaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa            >  2:2052332/1‑148 (MQ=255)
                                                                                                                                    acaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGaa           <  1:2538882/148‑1 (MQ=255)
                                                                                                                                    acaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGACTTGCAGATTGAAGAGGTGGaa           <  2:301018/148‑1 (MQ=255)
                                                                                                                                       gtgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGc        <  2:176323/148‑1 (MQ=255)
                                                                                                                                       gtgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGc        >  1:1314639/1‑148 (MQ=255)
                                                                                                                                        tgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGcc       >  2:2343783/1‑148 (MQ=255)
                                                                                                                                             aGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGcc  >  2:1063788/1‑148 (MQ=255)
                                                                                                                                             aGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGcc  <  2:1625031/148‑1 (MQ=255)
                                                                                                                                             aGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGcc  <  1:1104119/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCC  >  CP009273/3825557‑3825845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTG  >  CP009273/3825556‑3825851
                                                                                                                                                  |                                                                                                                                                     
GTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTC                                                                                                                                                    >  SRR19973069.1819083/1‑150 (MQ=60)
GTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTC                                                                                                                                                    >  SRR19973069.3064746/1‑150 (MQ=60)
GTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTC                                                                                                                                                    >  SRR19973069.2239599/1‑150 (MQ=60)
     CGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTC                                                                                                                                               <  SRR19973069.602370/150‑1 (MQ=60)
      GGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCG                                                                                                                                              >  SRR19973069.313668/1‑150 (MQ=60)
        CATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCC                                                                                                                                            >  SRR19973069.3065516/1‑150 (MQ=60)
            CCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCA                                                                                                                                        >  SRR19973069.3412300/1‑150 (MQ=60)
             CATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAC                                                                                                                                       >  SRR19973069.3678114/1‑150 (MQ=60)
             CATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCAC                                                                                                                                       >  SRR19973069.1812826/1‑150 (MQ=60)
              ATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACC                                                                                                                                      >  SRR19973069.1195119/1‑150 (MQ=60)
              ATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACC                                                                                                                                      >  SRR19973069.2094484/1‑150 (MQ=60)
               TCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCA                                                                                                                                     <  SRR19973069.353484/150‑1 (MQ=60)
                 AATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGT                                                                                                                                   <  SRR19973069.2642836/150‑1 (MQ=60)
                 AATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGT                                                                                                                                   <  SRR19973069.3528950/150‑1 (MQ=60)
                 AATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGT                                                                                                                                   <  SRR19973069.3528955/150‑1 (MQ=60)
                  ATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTC                                                                                                                                  >  SRR19973069.3809204/1‑150 (MQ=60)
                     GATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACA                                                                                                                               >  SRR19973069.1611575/1‑150 (MQ=60)
                      ATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAG                                                                                                                              >  SRR19973069.3378229/1‑150 (MQ=60)
                               TAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGT                                                                                                                     >  SRR19973069.3175480/1‑150 (MQ=60)
                               TAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGT                                                                                                                     >  SRR19973069.1636078/1‑150 (MQ=60)
                                AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACACCAGTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                    <  SRR19973069.2142065/150‑1 (MQ=60)
                                AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                    <  SRR19973069.2999435/150‑1 (MQ=60)
                                AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                    <  SRR19973069.1898388/150‑1 (MQ=60)
                                AATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                    <  SRR19973069.1898430/150‑1 (MQ=60)
                                AATTGTCAGCGCAGTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTT                                                                                                                    <  SRR19973069.2167007/150‑1 (MQ=60)
                                    GTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCA                                                                                                                <  SRR19973069.2087834/150‑1 (MQ=60)
                                    GTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCA                                                                                                                <  SRR19973069.2087816/150‑1 (MQ=60)
                                    GTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCA                                                                                                                <  SRR19973069.2087642/150‑1 (MQ=60)
                                     TCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAG                                                                                                               >  SRR19973069.1765659/1‑150 (MQ=60)
                                      CAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGT                                                                                                              >  SRR19973069.2383400/1‑150 (MQ=60)
                                          GCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGT                                                                                                          >  SRR19973069.1903761/1‑150 (MQ=60)
                                          GCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGT                                                                                                          >  SRR19973069.3804568/1‑150 (MQ=60)
                                           CATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTG                                                                                                         <  SRR19973069.3402539/150‑1 (MQ=60)
                                            ATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGC                                                                                                        >  SRR19973069.718070/1‑150 (MQ=60)
                                             TTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCT                                                                                                       <  SRR19973069.3121441/150‑1 (MQ=60)
                                               CCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGC                                                                                                     >  SRR19973069.1746671/1‑150 (MQ=60)
                                               CCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGC                                                                                                     >  SRR19973069.3139440/1‑150 (MQ=60)
                                               CCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGC                                                                                                     >  SRR19973069.186695/1‑150 (MQ=60)
                                                 CTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACCTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATAGTGTTGCCAGTACGTGCTGCTT                                                                                                   <  SRR19973069.3367879/150‑1 (MQ=60)
                                                 CTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTT                                                                                                   <  SRR19973069.6998/150‑1 (MQ=60)
                                                 CTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTT                                                                                                   <  SRR19973069.564314/150‑1 (MQ=60)
                                                  TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                                  <  SRR19973069.489385/150‑1 (MQ=60)
                                                  TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                                  <  SRR19973069.1598963/150‑1 (MQ=60)
                                                  TTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTT                                                                                                  <  SRR19973069.1598899/150‑1 (MQ=60)
                                                   TGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTT                                                                                                 <  SRR19973069.2559252/150‑1 (MQ=60)
                                                   TGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTT                                                                                                 >  SRR19973069.3047818/1‑150 (MQ=60)
                                                    GAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTA                                                                                                >  SRR19973069.915007/1‑150 (MQ=60)
                                                    GAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTA                                                                                                >  SRR19973069.915017/1‑150 (MQ=60)
                                                        CTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTGCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGT                                                                                            >  SRR19973069.2002852/1‑150 (MQ=60)
                                                              CACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGAT                                                                                      >  SRR19973069.2952047/1‑150 (MQ=60)
                                                              CACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGAT                                                                                      >  SRR19973069.2951939/1‑150 (MQ=60)
                                                              CACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGAT                                                                                      >  SRR19973069.183198/1‑150 (MQ=60)
                                                               ACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATC                                                                                     <  SRR19973069.519788/150‑1 (MQ=60)
                                                               ACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATC                                                                                     <  SRR19973069.2453026/150‑1 (MQ=60)
                                                                CCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCA                                                                                    <  SRR19973069.2630515/150‑1 (MQ=60)
                                                                CCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCA                                                                                    <  SRR19973069.3471767/150‑1 (MQ=60)
                                                                 CAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAC                                                                                   <  SRR19973069.221395/150‑1 (MQ=60)
                                                                 CAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAC                                                                                   >  SRR19973069.2072764/1‑150 (MQ=60)
                                                                 CAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAC                                                                                   <  SRR19973069.221311/150‑1 (MQ=60)
                                                                      CCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATC                                                                              >  SRR19973069.3541078/1‑150 (MQ=60)
                                                                            CACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAG                                                                        >  SRR19973069.2772009/1‑150 (MQ=60)
                                                                                TTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGC                                                                    <  SRR19973069.3207287/150‑1 (MQ=60)
                                                                                          GAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCA                                                          >  SRR19973069.2139182/1‑150 (MQ=60)
                                                                                          GAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCA                                                          <  SRR19973069.113756/150‑1 (MQ=60)
                                                                                          GAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCA                                                          >  SRR19973069.1442166/1‑150 (MQ=60)
                                                                                               GTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAC                                                     >  SRR19973069.644841/1‑150 (MQ=60)
                                                                                               GTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAC                                                     >  SRR19973069.1532811/1‑150 (MQ=60)
                                                                                               GTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGAC                                                     >  SRR19973069.644795/1‑150 (MQ=60)
                                                                                                   TGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCT                                                 <  SRR19973069.928222/150‑1 (MQ=60)
                                                                                                    GCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTT                                                >  SRR19973069.348203/1‑150 (MQ=60)
                                                                                                    GCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTT                                                <  SRR19973069.516691/150‑1 (MQ=60)
                                                                                                    GCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTT                                                <  SRR19973069.2309625/150‑1 (MQ=60)
                                                                                                     CAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCATAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTC                                               <  SRR19973069.2236272/150‑1 (MQ=60)
                                                                                                     CAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTC                                               >  SRR19973069.3561209/1‑150 (MQ=60)
                                                                                                       GACCATGTACTTTCACAACCTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCAT                                             <  SRR19973069.1658511/150‑1 (MQ=60)
                                                                                                        ACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATG                                            >  SRR19973069.1177889/1‑150 (MQ=60)
                                                                                                        ACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATG                                            >  SRR19973069.1909593/1‑150 (MQ=60)
                                                                                                          CATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCC                                          <  SRR19973069.3013413/150‑1 (MQ=60)
                                                                                                          CATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCC                                          <  SRR19973069.1095711/150‑1 (MQ=60)
                                                                                                          CATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCC                                          >  SRR19973069.1817990/1‑150 (MQ=60)
                                                                                                          CATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCC                                          >  SRR19973069.2554274/1‑150 (MQ=60)
                                                                                                          CATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCC                                          >  SRR19973069.2554273/1‑150 (MQ=60)
                                                                                                          CATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCC                                          >  SRR19973069.2001739/1‑150 (MQ=60)
                                                                                                            TTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCG                                        <  SRR19973069.3358817/150‑1 (MQ=60)
                                                                                                               ACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCT                                     >  SRR19973069.901868/1‑150 (MQ=60)
                                                                                                               ACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCT                                     <  SRR19973069.3717897/150‑1 (MQ=60)
                                                                                                               ACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCT                                     <  SRR19973069.3634947/150‑1 (MQ=60)
                                                                                                                CTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTT                                    >  SRR19973069.205210/1‑150 (MQ=60)
                                                                                                                    CACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAG                                <  SRR19973069.1454067/150‑1 (MQ=60)
                                                                                                                    CACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGACCGTCTTGCAG                                <  SRR19973069.3407952/150‑1 (MQ=60)
                                                                                                                      CAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAT                              >  SRR19973069.717278/1‑150 (MQ=60)
                                                                                                                      CAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAT                              >  SRR19973069.585670/1‑150 (MQ=60)
                                                                                                                        ACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTG                            <  SRR19973069.1538393/150‑1 (MQ=60)
                                                                                                                         CATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGA                           >  SRR19973069.2391558/1‑150 (MQ=60)
                                                                                                                             GCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAG                       <  SRR19973069.3699089/150‑1 (MQ=60)
                                                                                                                                 AGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGG                   >  SRR19973069.198830/1‑150 (MQ=60)
                                                                                                                                 AGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGG                   >  SRR19973069.2585247/1‑150 (MQ=60)
                                                                                                                                     ACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATG               <  SRR19973069.2555693/150‑1 (MQ=60)
                                                                                                                                      CAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGC              >  SRR19973069.1323643/1‑150 (MQ=60)
                                                                                                                                              AGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTT      >  SRR19973069.481467/1‑150 (MQ=60)
                                                                                                                                              AGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTT      <  SRR19973069.1111832/150‑1 (MQ=60)
                                                                                                                                               GTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC     >  SRR19973069.2250552/1‑150 (MQ=60)
                                                                                                                                               GTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC     >  SRR19973069.899075/1‑150 (MQ=60)
                                                                                                                                               GTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC     >  SRR19973069.2250489/1‑150 (MQ=60)
                                                                                                                                               GTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC     >  SRR19973069.2824860/1‑150 (MQ=60)
                                                                                                                                               GTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC     >  SRR19973069.2958555/1‑150 (MQ=60)
                                                                                                                                                  ATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTG  >  SRR19973069.3818405/1‑150 (MQ=60)
                                                                                                                                                  ATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTG  >  SRR19973069.3818376/1‑150 (MQ=60)
                                                                                                                                                  ATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCAGTCAGCAGCGGGCACTTCACAGACGGCTACATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCTTGCCTTCGTG  >  SRR19973069.830294/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
GTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTG  >  CP009273/3825556‑3825851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑