Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,224,391 G→T 34.1% V236F (GTT→TTT)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,224,3910GT34.1% 379.5 / 199.9 234V236F (GTT→TTT) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (84/70);  new base T (48/32);  total (132/102)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

ACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCT  >  CP009273/4224244‑4224535
                                                                                                                                                   |                                                                                                                                                
aCTCACATCGCGGAAGTGCTGAAAAAATAAAACCCGCAAACCCCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACg                                                                                                                                                  <  1:678365/148‑1 (MQ=255)
aCTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACg                                                                                                                                                  <  2:919643/148‑1 (MQ=255)
aCTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACg                                                                                                                                                  <  2:2279451/148‑1 (MQ=255)
   cacaTCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTg                                                                                                                                               >  1:1545669/1‑148 (MQ=255)
        cgcgGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCaaaa                                                                                                                                          >  2:627610/1‑148 (MQ=255)
          cgtaaGTGTTTAAAAAATTAAACCCGCAAACCACGCTGTTGTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAaca                                                                                                                                        <  1:3357313/145‑1 (MQ=255)
            gAAGTGCTTTAAAAAGTAAACCCGGAAACCACGCTGTTATTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACt                                                                                                                                      <  2:890671/148‑1 (MQ=255)
            gAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATCGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACt                                                                                                                                      >  1:1015314/1‑148 (MQ=255)
            gAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACt                                                                                                                                      <  2:1447977/148‑1 (MQ=255)
             aaGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACtt                                                                                                                                     >  2:1082230/1‑148 (MQ=255)
             aaGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACtt                                                                                                                                     >  1:766686/1‑148 (MQ=255)
             aaGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACtt                                                                                                                                     >  1:272963/1‑148 (MQ=255)
               gTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTg                                                                                                                                   >  1:540896/1‑148 (MQ=255)
               gTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTg                                                                                                                                   >  1:2375149/1‑148 (MQ=255)
                  cTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTgcgg                                                                                                                                >  1:1410566/1‑148 (MQ=255)
                     aaaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACCCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGgcgc                                                                                                                             <  2:189010/148‑1 (MQ=255)
                      aaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCt                                                                                                                            <  2:536149/148‑1 (MQ=255)
                          aGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTcc                                                                                                                        <  2:784669/148‑1 (MQ=255)
                           ttAACCCCTGAAACCACGGTGTTCGTCTAACCATCTAAAACCTACACCACTCTGTAAAATAGGACAAACGCCATAAGCGGGCGGGAATGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTcca                                                                                                                       <  1:2691095/147‑1 (MQ=255)
                              aaCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTccacca                                                                                                                    <  1:1698960/148‑1 (MQ=255)
                                 ccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATg                                                                                                                 >  1:893904/1‑148 (MQ=255)
                                 ccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATg                                                                                                                 >  1:3328030/1‑148 (MQ=255)
                                 ccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATg                                                                                                                 >  1:1567020/1‑148 (MQ=255)
                                     aaaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                                                                             >  2:1643571/1‑148 (MQ=255)
                                     aaaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                                                                             >  1:2401582/1‑148 (MQ=255)
                                       aCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAg                                                                                                           <  1:1366781/148‑1 (MQ=255)
                                        ccACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGc                                                                                                          >  1:3181360/1‑148 (MQ=255)
                                         cACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGcc                                                                                                         >  1:2777233/1‑148 (MQ=255)
                                          aCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAGCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCg                                                                                                        <  1:3529790/148‑1 (MQ=255)
                                          aCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCg                                                                                                        <  2:3406567/148‑1 (MQ=255)
                                           cGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                                                                                       >  1:2561932/1‑148 (MQ=255)
                                            gCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGtt                                                                                                      <  2:3221603/148‑1 (MQ=255)
                                             cTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTg                                                                                                     >  2:1297598/1‑148 (MQ=255)
                                                ttCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                                                                                  >  1:2861413/1‑148 (MQ=255)
                                                ttCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                                                                                  >  2:208414/1‑148 (MQ=255)
                                                 tCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGa                                                                                                 >  2:35393/1‑148 (MQ=255)
                                                      gTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                                                                            <  1:2720880/148‑1 (MQ=255)
                                                         gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGtc                                                                                         >  2:496148/1‑147 (MQ=255)
                                                         gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                                                                         >  2:1964407/1‑148 (MQ=255)
                                                          cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                        >  1:1662645/1‑148 (MQ=255)
                                                          cATCTAAAACATTCACCACACAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGTTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                        <  1:1322581/148‑1 (MQ=255)
                                                           aTCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAtt                                                                                       >  1:696449/1‑148 (MQ=255)
                                                              tAAAACCTTCACCACTCAGGAAACTATGACCCACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAt                                                                                    <  2:300112/148‑1 (MQ=255)
                                                                aaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                                                                                  <  2:3557148/148‑1 (MQ=255)
                                                                   ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGc                                                                               <  1:1567670/148‑1 (MQ=255)
                                                                   ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGc                                                                               >  2:586813/1‑148 (MQ=255)
                                                                    cTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGcc                                                                              <  1:865239/148‑1 (MQ=255)
                                                                     ttCACCACTCAGGAAACTATGACCACCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCa                                                                             <  1:2204169/148‑1 (MQ=255)
                                                                     ttCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTATGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCa                                                                             >  2:2344586/1‑148 (MQ=255)
                                                                      tCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTCTTGATACTGCCaa                                                                            >  2:752488/1‑148 (MQ=255)
                                                                      tCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCaa                                                                            >  1:1623996/1‑148 (MQ=255)
                                                                         ccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACAt                                                                         >  2:2252368/1‑148 (MQ=255)
                                                                          cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATg                                                                        <  2:1801314/148‑1 (MQ=255)
                                                                           acTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGt                                                                       <  1:2055162/148‑1 (MQ=255)
                                                                             tCAGGAAACTATGACCAACGCCCCTAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc                                                                     >  2:2231737/1‑148 (MQ=255)
                                                                             tCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc                                                                     >  2:1509732/1‑148 (MQ=255)
                                                                             tCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc                                                                     >  2:2119029/1‑148 (MQ=255)
                                                                                 gAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGt                                                                 >  1:807897/1‑148 (MQ=255)
                                                                                  aaaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGtt                                                                >  1:3136984/1‑148 (MQ=255)
                                                                                  aaaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGtt                                                                <  2:547554/148‑1 (MQ=255)
                                                                                   aaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTc                                                               >  1:402926/1‑148 (MQ=255)
                                                                                     cTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTg                                                             <  2:3375318/148‑1 (MQ=255)
                                                                                     cTATGACCAACGCCCAGAGCGCGCGGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTg                                                             <  2:1036850/148‑1 (MQ=255)
                                                                                      tATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTgg                                                            <  1:683355/148‑1 (MQ=255)
                                                                                       aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTggg                                                           >  1:1703084/1‑148 (MQ=255)
                                                                                       aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTggg                                                           >  1:2523689/1‑148 (MQ=255)
                                                                                        tGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGa                                                          >  1:958503/1‑148 (MQ=255)
                                                                                        tGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGa                                                          <  2:3209952/148‑1 (MQ=255)
                                                                                           ccAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTg                                                       <  1:1082230/148‑1 (MQ=255)
                                                                                              aCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                    <  1:1311578/148‑1 (MQ=255)
                                                                                              aCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                    <  1:710864/148‑1 (MQ=255)
                                                                                               cGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGtt                                                   <  2:2379988/148‑1 (MQ=255)
                                                                                                gCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTg                                                  <  1:520569/148‑1 (MQ=255)
                                                                                                 cccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTgg                                                 >  1:3519060/1‑148 (MQ=255)
                                                                                                  ccttAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggc                                                <  2:797945/145‑1 (MQ=255)
                                                                                                     tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggcggc                                             <  2:1089984/148‑1 (MQ=255)
                                                                                                      aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGcc                                            >  2:1662866/1‑148 (MQ=255)
                                                                                                        cgcgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGt                                          >  2:1795366/1‑148 (MQ=255)
                                                                                                          cgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTa                                        <  2:1205322/148‑1 (MQ=255)
                                                                                                              tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAcgct                                    >  1:1999850/1‑148 (MQ=255)
                                                                                                                 cTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtg                                 <  2:2820513/148‑1 (MQ=255)
                                                                                                                  tGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTTCTCTTtgt                                >  1:917372/1‑148 (MQ=255)
                                                                                                                   ggTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGTCTCTTtgtg                               >  1:3022948/1‑148 (MQ=255)
                                                                                                                           aaaGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGa                       <  2:1545669/148‑1 (MQ=255)
                                                                                                                             aGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCGTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAtt                     <  1:627610/148‑1 (MQ=255)
                                                                                                                                 gCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGcc                 >  1:216052/1‑148 (MQ=255)
                                                                                                                                    ggTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGt              <  2:1722194/148‑1 (MQ=255)
                                                                                                                                     gTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCGTTGTGGTCAGCGATTGGCCTGTc             >  2:2146800/1‑148 (MQ=255)
                                                                                                                                       gatgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa           <  2:272919/148‑1 (MQ=255)
                                                                                                                                       gatgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa           >  2:2611908/1‑148 (MQ=255)
                                                                                                                                        atgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt          >  2:2322622/1‑148 (MQ=255)
                                                                                                                                        atgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGTCTCTTTGTGGTGAGCGATTGGCCTGTCGAt          >  1:637618/1‑148 (MQ=255)
                                                                                                                                          gaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTg        <  1:1924507/148‑1 (MQ=255)
                                                                                                                                          gaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTg        <  1:826086/148‑1 (MQ=255)
                                                                                                                                          gaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTg        <  1:1108746/148‑1 (MQ=255)
                                                                                                                                          gaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTg        >  2:2139985/1‑148 (MQ=255)
                                                                                                                                              tacacTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtct    >  2:401071/2‑148 (MQ=255)
                                                                                                                                              aaCACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtct    >  2:3171099/1‑148 (MQ=255)
                                                                                                                                              aaCACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtct    >  1:2280217/1‑148 (MQ=255)
                                                                                                                                                cacGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctct  <  1:2686896/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCT  >  CP009273/4224244‑4224535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A