Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,224,391 G→T 60.7% V236F (GTT→TTT)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,224,3910GT60.7% 54.6 / 264.0 194V236F (GTT→TTT) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (39/37);  new base T (58/60);  total (97/97)
Fisher's exact test for biased strand distribution p-value = 8.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 199 total aligned reads displayed.

CTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTC  >  CP009273/4224245‑4224536
                                                                                                                                                  |                                                                                                                                                 
cTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGt                                                                                                                                                  <  2:473434/148‑1 (MQ=255)
 tCACATCGCGGAAGTGATTAAAAAAGTAAACCCGCAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACttt                                                                                                                                                 <  1:97197/148‑1 (MQ=255)
    caTCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCa                                                                                                                                              >  1:1606328/1‑148 (MQ=255)
    caTCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCa                                                                                                                                              >  1:1606025/1‑148 (MQ=255)
       cgcgGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCaaaa                                                                                                                                           >  1:651860/1‑148 (MQ=255)
         cgtaaGTGCTGAAAAAATTAAACCCCCAAACCACGGTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAaca                                                                                                                                         <  1:1642098/145‑1 (MQ=255)
         cgGAAGTGCTTAAAAAATTAAACCCGCAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAaca                                                                                                                                         <  1:3402850/148‑1 (MQ=255)
            aaGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACtt                                                                                                                                      >  2:1706575/1‑148 (MQ=255)
               tGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTgc                                                                                                                                   >  2:596004/1‑148 (MQ=255)
                gCTGAAAAAAGTAAACCCGTAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTgag                                                                                                                                  >  2:526934/1‑146 (MQ=255)
                    aaaaaaTTAAACCCGGAAACCACGCTGTTGTTGGTAGCATATAAAACCTTCACCACACAGGAAAATATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGgcgc                                                                                                                              <  1:818006/148‑1 (MQ=255)
                    aaaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGgcgc                                                                                                                              >  1:1946647/1‑148 (MQ=255)
                      aaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCtt                                                                                                                            >  1:2204242/1‑148 (MQ=255)
                        aaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTc                                                                                                                          <  2:3048259/148‑1 (MQ=255)
                           tAAACCCGGAAACCACGATTTTCTTGTTAGCATCTAAAACCTTCACCACTCAGGAAACTAGGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTccac                                                                                                                       <  1:452218/148‑1 (MQ=255)
                            aaaCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTccacc                                                                                                                      >  1:476511/1‑148 (MQ=255)
                               cccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAAt                                                                                                                   >  1:1510272/1‑148 (MQ=255)
                                ccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATg                                                                                                                  <  2:128203/148‑1 (MQ=255)
                                ccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATg                                                                                                                  >  1:361363/1‑148 (MQ=255)
                                    aaaCCACGCTGTTGGTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATCGCGCGCGTGACGGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                                                                              <  1:744748/148‑1 (MQ=255)
                                      aCCACGCTGTTGTTGGTAGCATCTAAAACCTTCACCACTCAGGAAAATATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAg                                                                                                            <  1:1558614/148‑1 (MQ=255)
                                      aCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAg                                                                                                            <  2:2443758/148‑1 (MQ=255)
                                          cGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                                                                                        <  2:1669458/148‑1 (MQ=255)
                                          cGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGt                                                                                                        >  1:2790723/1‑148 (MQ=255)
                                             tGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                                                                                     <  2:3381607/148‑1 (MQ=255)
                                             tGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                                                                                     <  2:1615274/148‑1 (MQ=255)
                                                  ttGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGt                                                                                                <  2:1049919/148‑1 (MQ=255)
                                                   tGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGtt                                                                                               <  2:3136328/148‑1 (MQ=255)
                                                      tAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTgg                                                                                            >  2:2139855/1‑148 (MQ=255)
                                                      tAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTgg                                                                                            >  2:344662/1‑148 (MQ=255)
                                                       aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                           >  1:3094009/1‑148 (MQ=255)
                                                       aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                           >  2:2829376/1‑148 (MQ=255)
                                                       aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                           <  1:1765003/148‑1 (MQ=255)
                                                       aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                           <  1:812507/148‑1 (MQ=255)
                                                        gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                                                                          <  1:2461790/148‑1 (MQ=255)
                                                         cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                         >  2:2173176/1‑148 (MQ=255)
                                                         cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                         <  1:1616399/148‑1 (MQ=255)
                                                         cATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                         >  1:2220956/1‑148 (MQ=255)
                                                           tCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTg                                                                                       >  2:3233190/1‑148 (MQ=255)
                                                           tCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTg                                                                                       <  2:3345409/148‑1 (MQ=255)
                                                            cTAAAACCTTCCCCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                                                                                      <  2:643510/148‑1 (MQ=255)
                                                            cTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGa                                                                                      <  2:1215119/148‑1 (MQ=255)
                                                              aaaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATa                                                                                    <  2:37890/148‑1 (MQ=255)
                                                               aaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                                                                                   >  1:1678258/1‑148 (MQ=255)
                                                                  ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGc                                                                                >  2:1110223/1‑148 (MQ=255)
                                                                       accacTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACa                                                                           <  1:1822967/148‑1 (MQ=255)
                                                                       accacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACa                                                                           <  1:1564246/148‑1 (MQ=255)
                                                                        ccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACAt                                                                          >  1:309965/1‑148 (MQ=255)
                                                                           cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTCTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt                                                                       >  2:458176/1‑148 (MQ=255)
                                                                                gAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGt                                                                  <  2:3011981/148‑1 (MQ=255)
                                                                                gAAACTATGACCAACGCCCAGAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGt                                                                  <  1:2255365/148‑1 (MQ=255)
                                                                                 aaaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGtt                                                                 <  1:1158111/148‑1 (MQ=255)
                                                                                      aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTggg                                                            <  2:1024704/148‑1 (MQ=255)
                                                                                        gACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGAc                                                          <  2:1374708/148‑1 (MQ=255)
                                                                                          ccAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTg                                                        >  1:568224/1‑148 (MQ=255)
                                                                                          ccAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTg                                                        <  1:1251561/148‑1 (MQ=255)
                                                                                           cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTgg                                                       >  2:2867709/1‑148 (MQ=255)
                                                                                           cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTgg                                                       >  2:2723656/1‑148 (MQ=255)
                                                                                           cAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTgg                                                       >  2:2719912/1‑148 (MQ=255)
                                                                                            aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTggg                                                      <  2:5840/148‑1 (MQ=255)
                                                                                            aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTggg                                                      >  2:3308789/1‑148 (MQ=255)
                                                                                            aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTggg                                                      <  2:1460289/148‑1 (MQ=255)
                                                                                             aCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAACACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                     <  2:3070060/148‑1 (MQ=255)
                                                                                             aCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                     >  1:2722135/1‑148 (MQ=255)
                                                                                              cGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAGTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGtt                                                    >  2:3286360/1‑148 (MQ=255)
                                                                                                cccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCACTGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTgg                                                  <  2:2338842/148‑1 (MQ=255)
                                                                                                    tcgcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggcggc                                              <  1:1025538/146‑1 (MQ=255)
                                                                                                     aGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGcc                                             >  1:2113172/1‑148 (MQ=255)
                                                                                                      gcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCg                                            <  1:3248610/148‑1 (MQ=255)
                                                                                                       cgcgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGt                                           >  2:3080281/1‑148 (MQ=255)
                                                                                                            gTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTActc                                      <  2:2679399/148‑1 (MQ=255)
                                                                                                             tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTActct                                     <  1:1985049/148‑1 (MQ=255)
                                                                                                             tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTActct                                     <  1:3346792/148‑1 (MQ=255)
                                                                                                             tGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTActct                                     >  1:1273792/1‑148 (MQ=255)
                                                                                                              gACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCtt                                    >  2:732220/1‑148 (MQ=255)
                                                                                                               aCTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCttt                                   >  1:1337357/1‑148 (MQ=255)
                                                                                                                  ggTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtgtg                                >  1:3312197/1‑148 (MQ=255)
                                                                                                                   gTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTgg                               <  1:245392/148‑1 (MQ=255)
                                                                                                                     tCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                             <  2:801012/148‑1 (MQ=255)
                                                                                                                         gAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCg                         >  1:3520958/1‑148 (MQ=255)
                                                                                                                          aaaGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGa                        <  2:675837/148‑1 (MQ=255)
                                                                                                                          aaaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGa                        <  2:2388453/148‑1 (MQ=255)
                                                                                                                           acgCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                       <  2:326440/146‑1 (MQ=255)
                                                                                                                           aaGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                       >  1:3113265/1‑148 (MQ=255)
                                                                                                                             gCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTg                     <  2:814630/148‑1 (MQ=255)
                                                                                                                               ggCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGc                   <  1:2173176/148‑1 (MQ=255)
                                                                                                                                gCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGcc                  <  2:159857/148‑1 (MQ=255)
                                                                                                                                 cAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCt                 >  1:2377288/1‑148 (MQ=255)
                                                                                                                                   ggTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGt               <  2:3496947/148‑1 (MQ=255)
                                                                                                                                     tgatgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCg             <  1:3396390/148‑1 (MQ=255)
                                                                                                                                      gatgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa            >  2:207659/1‑148 (MQ=255)
                                                                                                                                      gatgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTGTGTGGTCAGCGATTGGCCTGTCGa            >  2:3033899/1‑148 (MQ=255)
                                                                                                                                       atgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt           <  2:3539103/148‑1 (MQ=255)
                                                                                                                                         gaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTg         <  2:2204282/148‑1 (MQ=255)
                                                                                                                                           aaaacactttgcaaaacactttgcGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGtt       <  2:476511/148‑1 (MQ=255)
                                                                                                                                             aaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtct     >  1:1737074/1‑148 (MQ=255)
                                                                                                                                              acacTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctc    >  1:3488132/1‑148 (MQ=255)
                                                                                                                                              acacTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAATCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctc    >  2:2146896/1‑148 (MQ=255)
                                                                                                                                                acGTTGCATAACACTTTGCGGCGCTTTCCACCAATTCCAAAGCCGTTGGCGTTTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGTCTGGGTTGGCGGCCGGTTCTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctctc  <  1:2647313/148‑1 (MQ=255)
                                                                                                                                                acGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctctc  <  1:2139855/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTC  >  CP009273/4224245‑4224536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCA  >  CP009273/4224242‑4224538
                                                                                                                                                     |                                                                                                                                                   
GGACTCACATCGCGGAAGTGCTTAAAAAATTAAACCCGCAAACCACGGTGTTCTTGGTAGAAAATAAAACCTACACCACTCAGGAAACTATCACCAACGCCCATCGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACG                                                                                                                                                     <  SRR19973066.664755/150‑1 (MQ=60)
    TCACATCGCGGAAGTGCTGAAAAAATTAAACCCGCAAACCACGGGGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACGGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGC                                                                                                                                                 <  SRR19973066.97727/150‑1 (MQ=60)
    TCACATCGCGGAAGTGATTAAAAAAGTAAACCCGCAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGC                                                                                                                                                 <  SRR19973066.98241/150‑1 (MQ=60)
     CACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCA                                                                                                                                                >  SRR19973066.1617987/1‑150 (MQ=60)
     CACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCA                                                                                                                                                >  SRR19973066.1617980/1‑150 (MQ=60)
     CACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCA                                                                                                                                                >  SRR19973066.1617681/1‑150 (MQ=60)
     CACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCA                                                                                                                                                >  SRR19973066.1617677/1‑150 (MQ=60)
        ATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAA                                                                                                                                             >  SRR19973066.656604/1‑150 (MQ=60)
            CGTAAGTGCTGAAAAAATTAAACCCCCAAACCACGGTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACT                                                                                                                                         <  SRR19973066.1654046/150‑1 (MQ=60)
            CGGAAGTGCTTAAAAAATTAAACCCGCAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACT                                                                                                                                         <  SRR19973066.3425571/150‑1 (MQ=60)
            CGGAAGTGCTTAAAAAATTAAACCCGCAAACCACGCTGTTATTGGTAGAATCTAAAACCTTCACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACT                                                                                                                                         <  SRR19973066.1652938/150‑1 (MQ=60)
               atgtgattAAAAAATTAAACCCGGAAACCACGCTGTTTTTGGTAGAATCAAAAACCTTCACCACTAAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTG                                                                                                                                      <  SRR19973066.29179/142‑1 (MQ=60)
               AAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTG                                                                                                                                      >  SRR19973066.177604/1‑150 (MQ=60)
                  TGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCACCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGG                                                                                                                                   <  SRR19973066.461107/150‑1 (MQ=60)
                     TGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGC                                                                                                                                >  SRR19973066.1960435/1‑150 (MQ=60)
                       AAAAAATTAAACCCGGAAACCACGCTGTTGTTGGTAGCATATAAAACCTTCACCACACAGGAAAATATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTT                                                                                                                              <  SRR19973066.823989/150‑1 (MQ=60)
                       AAAAAATTAAACCCGGAAACCACGCTGTTCTTGGTAGAATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTT                                                                                                                              <  SRR19973066.822929/150‑1 (MQ=60)
                       AAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTT                                                                                                                              >  SRR19973066.2219560/1‑150 (MQ=60)
                       AAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTT                                                                                                                              >  SRR19973066.2219520/1‑150 (MQ=60)
                             GTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACC                                                                                                                        >  SRR19973066.480103/1‑150 (MQ=60)
                              TAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCA                                                                                                                       <  SRR19973066.455143/150‑1 (MQ=60)
                              TAAACCCGGAAACCACGATTTTCTTGTTAGCATCTAAAACCTTCACCACTCAGGAAACTAGGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCA                                                                                                                       <  SRR19973066.455695/150‑1 (MQ=60)
                                AACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAAT                                                                                                                     >  SRR19973066.1520841/1‑150 (MQ=60)
                                AACCCGCAAACCACGGTGTTCTTGTTAGCAACTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAAT                                                                                                                     <  SRR19973066.937477/150‑1 (MQ=60)
                                 ACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATG                                                                                                                    >  SRR19973066.364192/1‑150 (MQ=60)
                                  CCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGC                                                                                                                   >  SRR19973066.590366/1‑150 (MQ=60)
                                   CCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCC                                                                                                                  >  SRR19973066.2151780/1‑150 (MQ=60)
                                       AAACCACGCTGTTGGTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATCGCGCGCGTGACGGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAG                                                                                                              <  SRR19973066.750276/150‑1 (MQ=60)
                                        AACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGC                                                                                                             >  SRR19973066.3562622/1‑150 (MQ=60)
                                         ACCACGCTGTTGTTGGTAGCATCTAAAACCTTCACCACTCAGGAAAATATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCC                                                                                                            <  SRR19973066.1569822/150‑1 (MQ=60)
                                         ACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCACCGCCCATCGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCC                                                                                                            <  SRR19973066.1569830/150‑1 (MQ=60)
                                          CCACGCTGTTCTTGGTAGCATCTAAAACCTACACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCG                                                                                                           <  SRR19973066.1262984/150‑1 (MQ=60)
                                           CACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGT                                                                                                          >  SRR19973066.680781/1‑150 (MQ=60)
                                           CACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGT                                                                                                          >  SRR19973066.2809503/1‑150 (MQ=60)
                                           CACGCTGTTCTTGGTAGCATCTAAAACCTACACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGT                                                                                                          <  SRR19973066.2426291/150‑1 (MQ=60)
                                                 GTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGA                                                                                                    >  SRR19973066.3355868/1‑150 (MQ=60)
                                                       GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGG                                                                                              >  SRR19973066.1291150/1‑150 (MQ=60)
                                                        GTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGT                                                                                             >  SRR19973066.3115019/1‑150 (MQ=60)
                                                        GTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGT                                                                                             >  SRR19973066.3114674/1‑150 (MQ=60)
                                                        GTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGT                                                                                             >  SRR19973066.982209/1‑150 (MQ=60)
                                                          AGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAT                                                                                           <  SRR19973066.1777661/150‑1 (MQ=60)
                                                          AGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAT                                                                                           >  SRR19973066.1682474/1‑150 (MQ=60)
                                                          AGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAT                                                                                           >  SRR19973066.2236319/1‑150 (MQ=60)
                                                          AGCATCTAAAACCTTCACCACACAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAT                                                                                           <  SRR19973066.818464/150‑1 (MQ=60)
                                                           GCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATT                                                                                          <  SRR19973066.2478569/150‑1 (MQ=60)
                                                           GCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATT                                                                                          <  SRR19973066.2478557/150‑1 (MQ=60)
                                                            CATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTG                                                                                         <  SRR19973066.1628126/150‑1 (MQ=60)
                                                               CTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATA                                                                                      >  SRR19973066.2776714/1‑150 (MQ=60)
                                                                TAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAC                                                                                     >  SRR19973066.1692362/1‑150 (MQ=60)
                                                                TAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAC                                                                                     >  SRR19973066.1690477/1‑150 (MQ=60)
                                                                         CACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACAT                                                                            >  SRR19973066.312233/1‑150 (MQ=60)
                                                                          ACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATG                                                                           <  SRR19973066.1835965/150‑1 (MQ=60)
                                                                          ACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATG                                                                           <  SRR19973066.1575513/150‑1 (MQ=60)
                                                                                   GAAACTATGACCAACGCCCAGAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTC                                                                  <  SRR19973066.2270911/150‑1 (MQ=60)
                                                                                    AAACTATGACCAACGCCCATCGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCT                                                                 <  SRR19973066.1843464/150‑1 (MQ=60)
                                                                                    AAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCT                                                                 <  SRR19973066.1166340/150‑1 (MQ=60)
                                                                                     AACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTG                                                                <  SRR19973066.795788/150‑1 (MQ=60)
                                                                                         ATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGAC                                                            >  SRR19973066.2728596/1‑150 (MQ=60)
                                                                                           GACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTG                                                          >  SRR19973066.572244/1‑150 (MQ=60)
                                                                                             CCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGG                                                        <  SRR19973066.1260555/150‑1 (MQ=60)
                                                                                              CAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGT                                                       >  SRR19973066.2740446/1‑150 (MQ=60)
                                                                                                     CATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGC                                                >  SRR19973066.1972478/1‑150 (MQ=60)
                                                                                                     CATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGC                                                >  SRR19973066.2267175/1‑150 (MQ=60)
                                                                                                      ATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCC                                               >  SRR19973066.1832584/1‑150 (MQ=60)
                                                                                                      ATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCC                                               >  SRR19973066.2127779/1‑150 (MQ=60)
                                                                                                       TCGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCG                                              <  SRR19973066.1033089/150‑1 (MQ=60)
                                                                                                        CGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGT                                             <  SRR19973066.2377762/150‑1 (MQ=60)
                                                                                                         GCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTT                                            <  SRR19973066.3270018/150‑1 (MQ=60)
                                                                                                         GCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTT                                            <  SRR19973066.2173759/150‑1 (MQ=60)
                                                                                                         GCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGCTCGAGTTCTGGGACTGGGTTGGCGGCCGTT                                            <  SRR19973066.3270067/150‑1 (MQ=60)
                                                                                                              CGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCT                                       >  SRR19973066.1282988/1‑150 (MQ=60)
                                                                                                                TGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTT                                     >  SRR19973066.1347062/1‑150 (MQ=60)
                                                                                                                TGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTT                                     <  SRR19973066.3369073/150‑1 (MQ=60)
                                                                                                                TGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTT                                     <  SRR19973066.1999045/150‑1 (MQ=60)
                                                                                                                 GACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTG                                    <  SRR19973066.2980031/150‑1 (MQ=60)
                                                                                                                   CTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTG                                  >  SRR19973066.3334172/1‑150 (MQ=60)
                                                                                                                      GTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTC                               <  SRR19973066.1553528/150‑1 (MQ=60)
                                                                                                                      GTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTC                               <  SRR19973066.247298/150‑1 (MQ=60)
                                                                                                                          CTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCG                           >  SRR19973066.3544400/1‑150 (MQ=60)
                                                                                                                            GAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAT                         >  SRR19973066.3132944/1‑150 (MQ=60)
                                                                                                                            GAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAT                         >  SRR19973066.3134057/1‑150 (MQ=60)
                                                                                                                             AAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATT                        <  SRR19973066.315334/150‑1 (MQ=60)
                                                                                                                                  GGCAGGTGATGAAAAACACTTTGCAAACCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCT                   <  SRR19973066.2188268/150‑1 (MQ=60)
                                                                                                                                  GGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCT                   >  SRR19973066.2393338/1‑150 (MQ=60)
                                                                                                                                   GCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTGAGCGATTGGCCTG                  >  SRR19973066.2213896/1‑150 (MQ=60)
                                                                                                                                        TGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAGGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAT             <  SRR19973066.3419068/150‑1 (MQ=60)
                                                                                                                                        TGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAT             <  SRR19973066.3419042/150‑1 (MQ=60)
                                                                                                                                        TGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAT             <  SRR19973066.3419048/150‑1 (MQ=60)
                                                                                                                                        TGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTTGTGGTCAGCGATTGGCCTGTCGAT             >  SRR19973066.147814/1‑150 (MQ=60)
                                                                                                                                            GAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTT         >  SRR19973066.262102/1‑150 (MQ=60)
                                                                                                                                            GAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTT         <  SRR19973066.2090498/150‑1 (MQ=60)
                                                                                                                                              AAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCT       >  SRR19973066.1749614/1‑150 (MQ=60)
                                                                                                                                               AAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGATTtttata      >  SRR19973066.3511649/1‑144 (MQ=60)
                                                                                                                                               AAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTATC      >  SRR19973066.3511401/1‑150 (MQ=60)
                                                                                                                                               AAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTTTC      >  SRR19973066.493203/1‑150 (MQ=60)
                                                                                                                                               AAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGTCTCGTTGTGGTCAGCGATTGGCCTGTCGATTGTTATC      >  SRR19973066.1255879/1‑150 (MQ=60)
                                                                                                                                                 ACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTC    <  SRR19973066.1608342/150‑1 (MQ=60)
                                                                                                                                                   ACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGGATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTGTGTGGTGAGCGAGTGGGCTGGCGATTGTTgtgtgca  >  SRR19973066.1565047/1‑143 (MQ=60)
                                                                                                                                                   ACGTTGCATAACACTTTGCGGCGCTTTCCACCAATTCCAAAGCCGTTGGCGTTTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGTCTGGGTTGGCGGCCGGTTCTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCA  <  SRR19973066.2665095/150‑1 (MQ=60)
                                                                                                                                                   ACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCA  <  SRR19973066.347336/150‑1 (MQ=60)
                                                                                                                                                   ACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCA  <  SRR19973066.2154702/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCA  >  CP009273/4224242‑4224538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑