Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,224,391 G→T 100% V236F (GTT→TTT)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,224,3910GT95.8% 625.7 / 16.4 212V236F (GTT→TTT) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (3/6);  new base T (116/87);  total (119/93)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 218 total aligned reads displayed.

CTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTC  >  CP009273/4224245‑4224534
                                                                                                                                                  |                                                                                                                                               
cTCACATCGCGGAAGTGCTTAAAAAATTAAACCCGCACACCACGCTTTTCTTGGTAGCATCAAAAACCTTCACCCCTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGt                                                                                                                                                <  1:2444429/148‑1 (MQ=255)
 tCACATCGCGTATGTGATTAAAAAAGAAAACCCGCAAACCACGCTGTTCTTGGTAGCAACTAAACCCTACACCACTCAGTAAACTATCACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAACGCGGCAGGTGATGAAAAACACttt                                                                                                                                               <  1:906090/148‑1 (MQ=255)
  cacaTCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGACAGCGGCAGGTGATGAAAAACACTTTg                                                                                                                                              >  2:1814860/1‑148 (MQ=255)
    caTCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCa                                                                                                                                            >  2:2650372/1‑148 (MQ=255)
     aTCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCaa                                                                                                                                           >  1:386422/1‑148 (MQ=255)
       cgcgGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCaaaa                                                                                                                                         >  1:569877/1‑148 (MQ=255)
            aaGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACtt                                                                                                                                    <  2:688249/148‑1 (MQ=255)
             agagCTGAAAAAAGTAAACCAGGAAACCACCCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATCACCAACGCCCATAGCGCGCGTGACTGGATCCTGAAAGCGGCAGCTGATGAAAAACACTTTGCAAAACACtat                                                                                                                                   >  1:2940378/4‑146 (MQ=255)
             aGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACttt                                                                                                                                   >  2:886777/1‑148 (MQ=255)
                gCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTgcg                                                                                                                                >  1:1541002/1‑148 (MQ=255)
                   taaaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTgcggcg                                                                                                                             <  1:3321424/147‑1 (MQ=255)
                     aaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCt                                                                                                                           <  2:2561289/148‑1 (MQ=255)
                      aaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCtt                                                                                                                          >  2:2353241/1‑148 (MQ=255)
                        aaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTc                                                                                                                        <  1:1131876/148‑1 (MQ=255)
                        aaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTc                                                                                                                        >  1:1162719/1‑148 (MQ=255)
                         aGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTcc                                                                                                                       >  1:734577/1‑148 (MQ=255)
                          gTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCAAAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTcca                                                                                                                      >  2:1448910/1‑148 (MQ=255)
                           tAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAACCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTccac                                                                                                                     <  1:1436075/148‑1 (MQ=255)
                              aCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCaa                                                                                                                  <  1:3199862/148‑1 (MQ=255)
                              aCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCaa                                                                                                                  >  1:3494866/1‑148 (MQ=255)
                              aCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCaa                                                                                                                  >  2:2196512/1‑148 (MQ=255)
                              aCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTGTGCGGCGCTTTCCACCaa                                                                                                                  >  1:1991317/1‑148 (MQ=255)
                               cccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAAt                                                                                                                 >  1:982039/1‑148 (MQ=255)
                                   gAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCa                                                                                                             >  1:859060/1‑148 (MQ=255)
                                    aaaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                                                                            <  1:2417519/148‑1 (MQ=255)
                                    aaaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                                                                            <  1:3398426/148‑1 (MQ=255)
                                    aaaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCaa                                                                                                            >  2:2028814/1‑148 (MQ=255)
                                       ccACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGc                                                                                                         >  1:1403089/1‑148 (MQ=255)
                                            cTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTg                                                                                                    >  2:2686310/1‑148 (MQ=255)
                                              gTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                  >  1:1469598/1‑148 (MQ=255)
                                              gTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                  >  2:184253/1‑148 (MQ=255)
                                              gTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                  >  2:184067/1‑148 (MQ=255)
                                               ttCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                                                                                 >  2:837962/1‑148 (MQ=255)
                                                 cTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAg                                                                                               >  2:1495194/1‑148 (MQ=255)
                                                 cTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAg                                                                                               >  1:276240/1‑148 (MQ=255)
                                                     gTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                                                                           >  1:639322/1‑148 (MQ=255)
                                                      tAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTgg                                                                                          >  2:3187177/1‑148 (MQ=255)
                                                      tAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTGTgg                                                                                          >  2:474808/1‑148 (MQ=255)
                                                        gCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGGTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                                                                        <  2:2149268/148‑1 (MQ=255)
                                                           tCTAAAACCTTCACCACTCAGGAAACTATGACCACCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGATGGCGAGTTTGGTATTg                                                                                     <  2:3334229/148‑1 (MQ=255)
                                                             tAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAt                                                                                   <  1:728596/148‑1 (MQ=255)
                                                               aaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                                                                                 >  2:2462554/1‑148 (MQ=255)
                                                                  ccTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGc                                                                              >  1:171450/1‑148 (MQ=255)
                                                                   cTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGcc                                                                             >  2:2790529/1‑148 (MQ=255)
                                                                      caccccTCAGGAAACTATCACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACGGCCAAc                                                                          <  2:2391720/148‑1 (MQ=255)
                                                                      caccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAAc                                                                          <  2:628550/148‑1 (MQ=255)
                                                                       cccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACa                                                                         <  2:707460/147‑1 (MQ=255)
                                                                           cTCAGTAAACTATGACAAACGCCCATTGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCAACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt                                                                     <  1:1793585/148‑1 (MQ=255)
                                                                           cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt                                                                     <  2:1357930/148‑1 (MQ=255)
                                                                           cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt                                                                     >  1:2718891/1‑148 (MQ=255)
                                                                           cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt                                                                     >  1:3475108/1‑148 (MQ=255)
                                                                           cTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGtt                                                                     <  2:1617572/148‑1 (MQ=255)
                                                                             cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCg                                                                   >  1:3013523/1‑148 (MQ=255)
                                                                              aGGAAACTATGACCACCGCCCATAGCGCGCTTGACTGGTTCCTTAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGa                                                                  <  1:3077153/148‑1 (MQ=255)
                                                                                gAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGt                                                                >  1:1460254/1‑148 (MQ=255)
                                                                                 aaaCTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGtt                                                               >  1:2958793/1‑148 (MQ=255)
                                                                                    cTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTg                                                            <  2:3011707/148‑1 (MQ=255)
                                                                                      aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTggg                                                          >  2:522256/1‑148 (MQ=255)
                                                                                      aTGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTggg                                                          <  2:3424120/148‑1 (MQ=255)
                                                                                       tGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGa                                                         <  1:1046423/148‑1 (MQ=255)
                                                                                          ccAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTg                                                      >  1:980163/1‑148 (MQ=255)
                                                                                            aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTggg                                                    >  2:262721/1‑148 (MQ=255)
                                                                                            aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTggg                                                    <  2:1451914/148‑1 (MQ=255)
                                                                                             aCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                   >  2:2889734/1‑148 (MQ=255)
                                                                                             aCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                   >  2:1134689/1‑148 (MQ=255)
                                                                                             aCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGt                                                   >  2:1134328/1‑148 (MQ=255)
                                                                                               gCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTg                                                 <  1:2633243/148‑1 (MQ=255)
                                                                                                  cATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGCCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTGCTGGGACTGGGTTggcg                                              >  2:3240517/1‑148 (MQ=255)
                                                                                                    tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggcggc                                            <  1:1938760/148‑1 (MQ=255)
                                                                                                    tAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggcggc                                            >  2:2489198/1‑148 (MQ=255)
                                                                                                      gcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCg                                          >  2:1906550/1‑148 (MQ=255)
                                                                                                      gcgcgcGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCg                                          <  1:3045450/148‑1 (MQ=255)
                                                                                                       cgcgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGt                                         >  1:2572807/1‑148 (MQ=255)
                                                                                                           cgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAct                                     >  1:3115449/1‑148 (MQ=255)
                                                                                                                cTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtg                                >  1:2295761/1‑148 (MQ=255)
                                                                                                                cTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtg                                <  1:2333663/148‑1 (MQ=255)
                                                                                                                  ggTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTtgtg                              >  1:3227627/1‑148 (MQ=255)
                                                                                                                   gTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTgg                             <  2:2106619/148‑1 (MQ=255)
                                                                                                                    ttCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGt                            >  1:770922/1‑148 (MQ=255)
                                                                                                                           aaGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                     <  2:1403089/148‑1 (MQ=255)
                                                                                                                               ggCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGc                 <  1:1134647/148‑1 (MQ=255)
                                                                                                                                gCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGcc                >  1:1140465/1‑148 (MQ=255)
                                                                                                                                   ggTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGt             <  1:3021386/148‑1 (MQ=255)
                                                                                                                                    gTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTc            >  1:779896/1‑148 (MQ=255)
                                                                                                                                    gTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTc            >  2:3509914/1‑148 (MQ=255)
                                                                                                                                    gTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTc            >  2:1231142/1‑148 (MQ=255)
                                                                                                                                      gatgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa          <  1:162693/148‑1 (MQ=255)
                                                                                                                                      gatgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa          <  2:859060/148‑1 (MQ=255)
                                                                                                                                        tgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGAtt        >  1:2639286/1‑148 (MQ=255)
                                                                                                                                        tgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAtt        >  2:3248792/1‑148 (MQ=255)
                                                                                                                                         gaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTGATCGATTTGCCTGTCGAttt       >  1:226821/1‑147 (MQ=255)
                                                                                                                                         gaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCGTGTGGGTGAGCGGTTTGGCTGTCGATTg       >  1:1220197/1‑148 (MQ=255)
                                                                                                                                          aaaaaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGt      >  2:1192147/1‑148 (MQ=255)
                                                                                                                                          aaaaaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGt      >  2:2019023/1‑148 (MQ=255)
                                                                                                                                          aaaaaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAATCTTTGTGGTCAGCGATTGGCCTGTCGATTGt      >  2:2941698/1‑148 (MQ=255)
                                                                                                                                           aaaacactttgcaaaacactttgcGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGtt     >  2:3054918/1‑148 (MQ=255)
                                                                                                                                           aaaacactttgcaaaacactttgcGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGtt     >  1:1475977/1‑148 (MQ=255)
                                                                                                                                            aaaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtc    <  2:569877/148‑1 (MQ=255)
                                                                                                                                             aaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACGCTTTGTGGTGAGCGGTTGTGCTGTCGATGGttag   >  1:627171/1‑146 (MQ=255)
                                                                                                                                             aaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAAAGCCAAACCCCTTGGCGAGATTCGAATTGATACTGCCAAAAAGTTCGAGTTCTGGGACCGGGTTGGAGGCCGTAACTCTTTGGGGTCAGCCATTCGCACGTCGagattact   >  1:2218827/1‑141 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTC  >  CP009273/4224245‑4224534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATC  >  CP009273/4224244‑4224540
                                                                                                                                                   |                                                                                                                                                     
actcactgggcgtatGTGCTGAAAAAAGTAAACCCGGAACCCACGGTGTTCTTGTTAGCATCTAAACCCTTCACCCCTCTGGAAACTAGGACCAACGCCCATAGCGCGCGGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTT                                                                                                                                                     <  SRR19973065.64016/135‑1 (MQ=60)
ACTCACATCGCGGAAGTGCTGAAAAAATTAAACCCGCAAACCACGCTGTTCTTGGTAGCATCTAAAACCTCCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTT                                                                                                                                                     <  SRR19973065.815157/150‑1 (MQ=60)
ACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTT                                                                                                                                                     <  SRR19973065.243310/150‑1 (MQ=60)
 CTCACATCGCGGAAGTGCTTAAAAAATTAAACCCGCACACCACGCTTTTCTTGGTAGCATCAAAAACCTTCACCCCTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTG                                                                                                                                                    <  SRR19973065.2459832/150‑1 (MQ=60)
  tcacagcgcgtatttgctaaaaaaataAAACCCGCAAACCACGCTGTTCTTGGTACAAACAAAAACCTACACCACTCAGCAAACTATGACCACCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGC                                                                                                                                                   <  SRR19973065.912559/123‑1 (MQ=60)
  TCACATCGCGTATGTGATTAAAAAAGAAAACCCGCAAACCACGCTGTTCTTGGTAGCAACTAAACCCTACACCACTCAGTAAACTATCACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAACGCGGCAGGTGATGAAAAACACTTTGC                                                                                                                                                   <  SRR19973065.911944/150‑1 (MQ=60)
    ACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAA                                                                                                                                                 >  SRR19973065.389118/1‑150 (MQ=60)
      ATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAA                                                                                                                                               >  SRR19973065.1101464/1‑150 (MQ=60)
      ATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAA                                                                                                                                               >  SRR19973065.573480/1‑150 (MQ=60)
           GGAAGTGCTGAAAAAATTAAACCCGGAAACCACGCTGTTGTTGGTAGCATCTAAAACCTTCACCACTCTGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTT                                                                                                                                          <  SRR19973065.1547508/150‑1 (MQ=60)
            GAAGAGCTGAAAAAAGTAAACCAGGAAACCACCCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATCACCAACGCCCATAGCGCGCGTGACTGGATCCTGAAAGCGGCAGCTGATGAAAAACACTTTGCAAAACACTAT                                                                                                                                         >  SRR19973065.2958220/1‑150 (MQ=60)
             AAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTG                                                                                                                                        >  SRR19973065.1787101/1‑150 (MQ=60)
               GTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCG                                                                                                                                      >  SRR19973065.1551187/1‑150 (MQ=60)
                    TAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCT                                                                                                                                 <  SRR19973065.3341685/150‑1 (MQ=60)
                      AAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTT                                                                                                                               >  SRR19973065.3445022/1‑150 (MQ=60)
                       AAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTC                                                                                                                              >  SRR19973065.1170157/1‑150 (MQ=60)
                        AAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCC                                                                                                                             >  SRR19973065.739405/1‑150 (MQ=60)
                        AAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCC                                                                                                                             >  SRR19973065.739490/1‑150 (MQ=60)
                        AAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCC                                                                                                                             >  SRR19973065.741556/1‑150 (MQ=60)
                         AAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCA                                                                                                                            >  SRR19973065.3030148/1‑150 (MQ=60)
                         AAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCA                                                                                                                            <  SRR19973065.1139223/150‑1 (MQ=60)
                          AGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCAC                                                                                                                           >  SRR19973065.2402656/1‑150 (MQ=60)
                            TAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAACCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCA                                                                                                                         <  SRR19973065.1445397/150‑1 (MQ=60)
                             AAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAA                                                                                                                        >  SRR19973065.3516140/1‑150 (MQ=60)
                             AAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAA                                                                                                                        >  SRR19973065.3516109/1‑150 (MQ=60)
                             AAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTGTGCGGCGCTTTCCACCAA                                                                                                                        >  SRR19973065.2004231/1‑150 (MQ=60)
                              AACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAAT                                                                                                                       >  SRR19973065.988556/1‑150 (MQ=60)
                               ACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATG                                                                                                                      <  SRR19973065.3425018/150‑1 (MQ=60)
                               ACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATG                                                                                                                      <  SRR19973065.3219131/150‑1 (MQ=60)
                                  CGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCA                                                                                                                   >  SRR19973065.864599/1‑150 (MQ=60)
                                    GAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAA                                                                                                                 <  SRR19973065.196164/150‑1 (MQ=60)
                                     AAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCCCCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAG                                                                                                                <  SRR19973065.2432748/150‑1 (MQ=60)
                                     AAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAG                                                                                                                <  SRR19973065.3419200/150‑1 (MQ=60)
                                     AAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAG                                                                                                                <  SRR19973065.2321996/150‑1 (MQ=60)
                                     AAACCACGCTGTTATTGTTAGCATATAAAACCTTCACCACTCAGTAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAG                                                                                                                <  SRR19973065.2434821/150‑1 (MQ=60)
                                      AACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGC                                                                                                               >  SRR19973065.1412286/1‑150 (MQ=60)
                                        CCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCG                                                                                                             >  SRR19973065.2500653/1‑150 (MQ=60)
                                          ACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTT                                                                                                           <  SRR19973065.1427346/150‑1 (MQ=60)
                                            GCTGTTCTGGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGG                                                                                                         <  SRR19973065.699319/150‑1 (MQ=60)
                                             CTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGC                                                                                                        >  SRR19973065.1479020/1‑150 (MQ=60)
                                                TTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAG                                                                                                     >  SRR19973065.278089/1‑150 (MQ=60)
                                                   TTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCACCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTT                                                                                                  <  SRR19973065.1927791/150‑1 (MQ=60)
                                                    TGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTG                                                                                                 >  SRR19973065.643511/1‑150 (MQ=60)
                                                    TGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTG                                                                                                 >  SRR19973065.1165852/1‑150 (MQ=60)
                                                     GGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGG                                                                                                >  SRR19973065.1222504/1‑150 (MQ=60)
                                                           ATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGA                                                                                          <  SRR19973065.2661630/150‑1 (MQ=60)
                                                            TCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAT                                                                                         <  SRR19973065.291046/150‑1 (MQ=60)
                                                              TAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAC                                                                                       <  SRR19973065.731166/150‑1 (MQ=60)
                                                              TAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGGGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAC                                                                                       <  SRR19973065.733476/150‑1 (MQ=60)
                                                                AAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTG                                                                                     <  SRR19973065.3143901/150‑1 (MQ=60)
                                                                 AACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGC                                                                                    >  SRR19973065.172822/1‑150 (MQ=60)
                                                                  ACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCC                                                                                   <  SRR19973065.2563334/150‑1 (MQ=60)
                                                                  ACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCC                                                                                   <  SRR19973065.2916799/150‑1 (MQ=60)
                                                                          CACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTT                                                                           >  SRR19973065.3496250/1‑150 (MQ=60)
                                                                          CACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTT                                                                           >  SRR19973065.2735805/1‑150 (MQ=60)
                                                                          CACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTT                                                                           >  SRR19973065.2735801/1‑150 (MQ=60)
                                                                           ACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTC                                                                          <  SRR19973065.1470453/150‑1 (MQ=60)
                                                                            CTCAGTAAACTATGACAAACGCCCATTGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCAACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG                                                                         <  SRR19973065.1805352/150‑1 (MQ=60)
                                                                            CTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG                                                                         >  SRR19973065.33455/1‑150 (MQ=60)
                                                                            CTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG                                                                         >  SRR19973065.3031978/1‑150 (MQ=60)
                                                                            CTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCG                                                                         <  SRR19973065.123782/150‑1 (MQ=60)
                                                                               AGGAAACTATGACCACCGCCCATAGCGCGCTTGACTGGTTCCTTAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGT                                                                      <  SRR19973065.3095948/150‑1 (MQ=60)
                                                                               AGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGT                                                                      >  SRR19973065.3222418/1‑150 (MQ=60)
                                                                               AGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGT                                                                      >  SRR19973065.1469642/1‑150 (MQ=60)
                                                                                GGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTT                                                                     >  SRR19973065.2976170/1‑150 (MQ=60)
                                                                                GGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTT                                                                     >  SRR19973065.2976861/1‑150 (MQ=60)
                                                                                     CTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGG                                                                <  SRR19973065.1602036/150‑1 (MQ=60)
                                                                                      TATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGA                                                               >  SRR19973065.3062191/1‑150 (MQ=60)
                                                                                        TGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACT                                                             <  SRR19973065.636022/150‑1 (MQ=60)
                                                                                        TGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACT                                                             <  SRR19973065.1053343/150‑1 (MQ=60)
                                                                                         GACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTG                                                            >  SRR19973065.986667/1‑150 (MQ=60)
                                                                                         GACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTG                                                            >  SRR19973065.987440/1‑150 (MQ=60)
                                                                                                GCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGC                                                     <  SRR19973065.2653906/150‑1 (MQ=60)
                                                                                                GCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGC                                                     <  SRR19973065.2649516/150‑1 (MQ=60)
                                                                                                 CCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCG                                                    >  SRR19973065.1315232/1‑150 (MQ=60)
                                                                                                     TAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCG                                                <  SRR19973065.1951445/150‑1 (MQ=60)
                                                                                                      AGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGT                                               >  SRR19973065.2588862/1‑150 (MQ=60)
                                                                                                      AGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGT                                               >  SRR19973065.453170/1‑150 (MQ=60)
                                                                                                       GCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTT                                              <  SRR19973065.3064086/150‑1 (MQ=60)
                                                                                                       GCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTT                                              <  SRR19973065.3064638/150‑1 (MQ=60)
                                                                                                          CGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACT                                           <  SRR19973065.777300/150‑1 (MQ=60)
                                                                                                          CGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACT                                           >  SRR19973065.3134339/1‑150 (MQ=60)
                                                                                                          CGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACT                                           >  SRR19973065.3134437/1‑150 (MQ=60)
                                                                                                               GACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTG                                      >  SRR19973065.2310217/1‑150 (MQ=60)
                                                                                                               GACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTG                                      >  SRR19973065.2309935/1‑150 (MQ=60)
                                                                                                                ACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGT                                     <  SRR19973065.906680/150‑1 (MQ=60)
                                                                                                                ACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGT                                     <  SRR19973065.906673/150‑1 (MQ=60)
                                                                                                                ACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGT                                     <  SRR19973065.2041843/150‑1 (MQ=60)
                                                                                                                 CTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTTGTG                                    >  SRR19973065.3247001/1‑150 (MQ=60)
                                                                                                                 CTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTG                                    <  SRR19973065.2348245/150‑1 (MQ=60)
                                                                                                                   GGTTCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGT                                  >  SRR19973065.776045/1‑150 (MQ=60)
                                                                                                                      TCCTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAG                               >  SRR19973065.3503249/1‑150 (MQ=60)
                                                                                                                        CTGAAAGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCG                             <  SRR19973065.1447371/150‑1 (MQ=60)
                                                                                                                             AGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGG                        <  SRR19973065.3039870/150‑1 (MQ=60)
                                                                                                                               CGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCC                      >  SRR19973065.3346111/1‑150 (MQ=60)
                                                                                                                               CGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCC                      >  SRR19973065.2645748/1‑150 (MQ=60)
                                                                                                                               CGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCC                      >  SRR19973065.1147841/1‑150 (MQ=60)
                                                                                                                                GGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCT                     <  SRR19973065.1141680/150‑1 (MQ=60)
                                                                                                                                GGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCT                     <  SRR19973065.1142002/150‑1 (MQ=60)
                                                                                                                                GGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCT                     <  SRR19973065.1142044/150‑1 (MQ=60)
                                                                                                                                 GCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTCTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTG                    <  SRR19973065.2152931/150‑1 (MQ=60)
                                                                                                                                   AGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCCGCGATTGGCCTGTC                  >  SRR19973065.785074/1‑150 (MQ=60)
                                                                                                                                    GGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTTCTCTTTGTGGTCAGCGATTGGCCTGTCG                 >  SRR19973065.3102332/1‑150 (MQ=60)
                                                                                                                                    GGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCG                 <  SRR19973065.3039898/150‑1 (MQ=60)
                                                                                                                                       GATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGATT              >  SRR19973065.2655582/1‑150 (MQ=60)
                                                                                                                                       GATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATT              <  SRR19973065.164026/150‑1 (MQ=60)
                                                                                                                                       GATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATT              <  SRR19973065.164028/150‑1 (MQ=60)
                                                                                                                                       GATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATT              <  SRR19973065.163502/150‑1 (MQ=60)
                                                                                                                                        ATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCgtgtgggtgagcggtttggctgtcgattg             >  SRR19973065.1228125/1‑121 (MQ=60)
                                                                                                                                        ATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTGATCGATTTGCCTGTCGATTT             >  SRR19973065.228483/1‑150 (MQ=60)
                                                                                                                                        ATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTG             >  SRR19973065.227508/1‑150 (MQ=60)
                                                                                                                                          GAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTT           >  SRR19973065.1485417/1‑150 (MQ=60)
                                                                                                                                          GAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTT           >  SRR19973065.1484491/1‑150 (MQ=60)
                                                                                                                                           AAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTTGTCGTTGTGGTCAGCGATTTGCCTGTCGGTTGTTA          >  SRR19973065.57651/1‑150 (MQ=60)
                                                                                                                                            AAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACGCTTTGTGGTgagcggttgtgctgtcgatggttag         >  SRR19973065.631250/1‑125 (MQ=60)
                                                                                                                                            AAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAAAGCCAAACCCCTTGGCGAGATTCGAATTGATACTGCCAAAAAGTTCGAGTTCTGGGACCGGGTTGGAGGCCGTAACTCTTTGGGGTCAGCcattcgcacgtcgagattact         >  SRR19973065.2232994/1‑129 (MQ=60)
                                                                                                                                               ACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTC      <  SRR19973065.85486/150‑1 (MQ=60)
                                                                                                                                                  CTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAT   <  SRR19973065.2586006/150‑1 (MQ=60)
                                                                                                                                                   TTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATC  <  SRR19973065.2210584/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
ACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATC  >  CP009273/4224244‑4224540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑