Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 300,770 C→A 92.3% V49V (GTG→GTT)  ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273300,7700CA92.3% 458.7 / 30.0 168V49V (GTG→GTT) ecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (4/9);  new base A (75/80);  total (79/89)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 169 total aligned reads displayed.

ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAC  >  CP009273/300625‑300914
                                                                                                                                                 |                                                                                                                                                
aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAc                                                                                                                                                >  2:3308831/1‑148 (MQ=255)
  tCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTa                                                                                                                                              >  2:114219/1‑148 (MQ=255)
   cGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTac                                                                                                                                             <  2:3200240/148‑1 (MQ=255)
    gCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                            <  1:2195706/148‑1 (MQ=255)
     ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTacac                                                                                                                                           <  1:2942330/148‑1 (MQ=255)
      tctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGTAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACTGTATATCCGTGCGCTTTTGTTGTTATTAACTACACg                                                                                                                                          <  1:2697671/148‑1 (MQ=255)
       ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                         <  1:1978512/148‑1 (MQ=255)
        tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGtt                                                                                                                                        >  1:806628/1‑148 (MQ=255)
        tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGtt                                                                                                                                        >  2:886813/1‑148 (MQ=255)
        tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGtt                                                                                                                                        <  2:3283229/148‑1 (MQ=255)
           ccAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGc                                                                                                                                     <  2:2022393/148‑1 (MQ=255)
           ccAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGc                                                                                                                                     >  2:1050378/1‑148 (MQ=255)
           ccAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCGTTTGTTGTTATTAACTACACGTTTGc                                                                                                                                     >  2:1049766/1‑148 (MQ=255)
            cAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCt                                                                                                                                    >  2:590377/1‑148 (MQ=255)
               ccTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGAc                                                                                                                                 >  2:941867/1‑148 (MQ=255)
                   cAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAag                                                                                                                             >  1:1767614/1‑148 (MQ=255)
                   cAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAag                                                                                                                             >  2:3535830/1‑148 (MQ=255)
                     aCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtc                                                                                                                           >  1:787791/1‑148 (MQ=255)
                       gCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCGATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcag                                                                                                                         <  1:1010673/148‑1 (MQ=255)
                        ccAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagt                                                                                                                        >  2:1068652/1‑148 (MQ=255)
                        ccAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagt                                                                                                                        <  1:2133327/148‑1 (MQ=255)
                         cAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagtc                                                                                                                       <  2:376461/148‑1 (MQ=255)
                          aGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtct                                                                                                                      <  2:2925364/148‑1 (MQ=255)
                          aGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtct                                                                                                                      <  2:1148177/148‑1 (MQ=255)
                             tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCCATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCgg                                                                                                                   <  2:1459953/148‑1 (MQ=255)
                             tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCgg                                                                                                                   <  2:2629461/148‑1 (MQ=255)
                             tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCgg                                                                                                                   <  2:3462294/148‑1 (MQ=255)
                             tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCgg                                                                                                                   <  1:529594/148‑1 (MQ=255)
                              ggCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                  <  1:122843/148‑1 (MQ=255)
                              ggCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                  <  2:2678127/148‑1 (MQ=255)
                              ggCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                  <  2:2678769/148‑1 (MQ=255)
                              ggCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGATTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                  <  1:3311286/148‑1 (MQ=255)
                                  gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGc                                                                                                              >  1:1904330/1‑148 (MQ=255)
                                   ggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCa                                                                                                             <  2:2627435/148‑1 (MQ=255)
                                     ggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGc                                                                                                           <  2:1244492/148‑1 (MQ=255)
                                     ggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGc                                                                                                           <  2:560416/148‑1 (MQ=255)
                                            aGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                    <  2:259936/148‑1 (MQ=255)
                                            aGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                    >  1:2014393/1‑148 (MQ=255)
                                            aGCAGGTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                    <  2:2060054/148‑1 (MQ=255)
                                              cAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTc                                                                                                  <  2:1577214/148‑1 (MQ=255)
                                               aGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCa                                                                                                 <  1:1913299/148‑1 (MQ=255)
                                                 ttCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGa                                                                                               >  1:163874/1‑148 (MQ=255)
                                                    aCCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTg                                                                                            >  1:3471046/1‑148 (MQ=255)
                                                      cATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGcc                                                                                          <  2:870539/148‑1 (MQ=255)
                                                       aTCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccg                                                                                         <  1:3364804/148‑1 (MQ=255)
                                                       aTCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccg                                                                                         >  2:1808136/1‑148 (MQ=255)
                                                         ccACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgac                                                                                       >  1:1857803/1‑148 (MQ=255)
                                                           aCCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccg                                                                                     >  1:1452487/1‑148 (MQ=255)
                                                             cGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGaa                                                                                   <  1:2238486/148‑1 (MQ=255)
                                                                tCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATc                                                                                >  2:276674/1‑148 (MQ=255)
                                                                   ggTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGcc                                                                             <  1:3412182/148‑1 (MQ=255)
                                                                      gcgcAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCtgt                                                                          >  2:3018829/1‑148 (MQ=255)
                                                                        gcAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGAAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTgtgt                                                                        <  1:284262/148‑1 (MQ=255)
                                                                                ctgctgCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTg                                                                >  2:2440997/1‑148 (MQ=255)
                                                                                ctgctgCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTg                                                                >  2:1710908/1‑148 (MQ=255)
                                                                                    tgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccgc                                                            <  1:1759626/148‑1 (MQ=255)
                                                                                       ccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAg                                                         >  1:2184680/1‑148 (MQ=255)
                                                                                          ggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAt                                                      <  1:3422935/148‑1 (MQ=255)
                                                                                          ggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAt                                                      <  2:423075/148‑1 (MQ=255)
                                                                                           gCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAACTCGCCTGTGTCAAAGGTGCCGCCAGCATa                                                     <  1:405712/148‑1 (MQ=255)
                                                                                            cTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAc                                                    >  2:2328240/1‑148 (MQ=255)
                                                                                             tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACa                                                   >  1:23541/1‑148 (MQ=255)
                                                                                             tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACa                                                   >  1:1828484/1‑148 (MQ=255)
                                                                                               tCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGa                                                 >  2:2969045/1‑148 (MQ=255)
                                                                                               tCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGa                                                 <  1:886813/148‑1 (MQ=255)
                                                                                                cAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                >  2:671441/1‑148 (MQ=255)
                                                                                                cAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                >  1:949888/1‑148 (MQ=255)
                                                                                                  aTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCg                                              >  2:599078/1‑148 (MQ=255)
                                                                                                  aTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCg                                              >  2:599023/1‑148 (MQ=255)
                                                                                                   tAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAATGTTGCCGCCAGCATACAGACCGc                                             >  2:3084195/1‑148 (MQ=255)
                                                                                                   tAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGc                                             <  2:3074048/148‑1 (MQ=255)
                                                                                                    aGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCt                                            <  1:155456/148‑1 (MQ=255)
                                                                                                      cACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGt                                          <  1:1317516/148‑1 (MQ=255)
                                                                                                       aCTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTc                                         <  2:1748044/148‑1 (MQ=255)
                                                                                                          gATGGCAATACGGTATATCCGTGCTCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagc                                      <  1:1732295/148‑1 (MQ=255)
                                                                                                          gATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagc                                      >  2:2327676/1‑148 (MQ=255)
                                                                                                           aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagca                                     <  2:2180280/148‑1 (MQ=255)
                                                                                                           aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagca                                     >  1:2469578/1‑148 (MQ=255)
                                                                                                              gCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAggg                                  <  2:477657/148‑1 (MQ=255)
                                                                                                              gCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAggg                                  >  2:1519969/1‑148 (MQ=255)
                                                                                                               cAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGc                                 <  1:1068652/148‑1 (MQ=255)
                                                                                                                  tACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCtt                              >  1:1467076/1‑148 (MQ=255)
                                                                                                                  tACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCtt                              >  2:1540437/1‑148 (MQ=255)
                                                                                                                   aCGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTa                             <  2:806628/148‑1 (MQ=255)
                                                                                                                    cGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAg                            >  1:1505259/1‑148 (MQ=255)
                                                                                                                     ggTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGt                           <  2:1139140/148‑1 (MQ=255)
                                                                                                                       tataTCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTa                         >  2:1218760/1‑148 (MQ=255)
                                                                                                                         tatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAAt                       <  2:2249151/148‑1 (MQ=255)
                                                                                                                           tCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGt                     <  2:1904330/148‑1 (MQ=255)
                                                                                                                           tCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGt                     <  2:1904336/148‑1 (MQ=255)
                                                                                                                           tCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGt                     <  1:561882/148‑1 (MQ=255)
                                                                                                                           tCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGt                     >  2:258265/1‑148 (MQ=255)
                                                                                                                            ccGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                    >  1:2993041/1‑148 (MQ=255)
                                                                                                                            ccGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                    >  1:1198419/1‑148 (MQ=255)
                                                                                                                                gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAATGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGc                >  2:1885559/1‑148 (MQ=255)
                                                                                                                                  gcTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCa              <  1:3027925/148‑1 (MQ=255)
                                                                                                                                    ttttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACg            <  1:465321/148‑1 (MQ=255)
                                                                                                                                    ttttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACg            >  1:2771200/1‑148 (MQ=255)
                                                                                                                                     tttGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGt           >  1:3288654/1‑148 (MQ=255)
                                                                                                                                              aTTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAc  <  2:726877/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAC  >  CP009273/300625‑300914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  CP009273/300623‑300919
                                                                                                                                                   |                                                                                                                                                     
ATACTCGCTCTCATCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAC                                                                                                                                                     >  SRR19973066.841925/1‑150 (MQ=60)
 TACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACT                                                                                                                                                    <  SRR19973066.1659011/150‑1 (MQ=60)
      GCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACG                                                                                                                                               <  SRR19973066.2210933/150‑1 (MQ=60)
      GCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACG                                                                                                                                               >  SRR19973066.238589/1‑150 (MQ=60)
       CTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGT                                                                                                                                              <  SRR19973066.2961881/150‑1 (MQ=60)
        TCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGTAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACTGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTT                                                                                                                                             <  SRR19973066.2715710/150‑1 (MQ=60)
        TCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTT                                                                                                                                             >  SRR19973066.1128295/1‑150 (MQ=60)
        TCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTT                                                                                                                                             >  SRR19973066.812552/1‑150 (MQ=60)
         CTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTT                                                                                                                                            <  SRR19973066.1993316/150‑1 (MQ=60)
         CTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTT                                                                                                                                            <  SRR19973066.1992485/150‑1 (MQ=60)
             CCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTG                                                                                                                                        <  SRR19973066.1856805/150‑1 (MQ=60)
                 GCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAA                                                                                                                                    <  SRR19973066.1911013/150‑1 (MQ=60)
                 CCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAA                                                                                                                                    <  SRR19973066.2993917/150‑1 (MQ=60)
                 CCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAA                                                                                                                                    <  SRR19973066.1909119/150‑1 (MQ=60)
                   TGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAG                                                                                                                                  >  SRR19973066.1780285/1‑150 (MQ=60)
                     CAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTC                                                                                                                                >  SRR19973066.793597/1‑150 (MQ=60)
                         GCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCGATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTC                                                                                                                            <  SRR19973066.1018121/150‑1 (MQ=60)
                          CCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCT                                                                                                                           <  SRR19973066.2148115/150‑1 (MQ=60)
                               TGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAC                                                                                                                      <  SRR19973066.533464/150‑1 (MQ=60)
                                GGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACG                                                                                                                     <  SRR19973066.124078/150‑1 (MQ=60)
                                GGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGATTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACG                                                                                                                     <  SRR19973066.3333252/150‑1 (MQ=60)
                                  CGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGC                                                                                                                   >  SRR19973066.1917912/1‑150 (MQ=60)
                                  CGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGC                                                                                                                   >  SRR19973066.1917906/1‑150 (MQ=60)
                                   GGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCA                                                                                                                  >  SRR19973066.725781/1‑150 (MQ=60)
                                   GGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCA                                                                                                                  <  SRR19973066.46042/150‑1 (MQ=60)
                                            AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         >  SRR19973066.2028503/1‑150 (MQ=60)
                                                 AGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGA                                                                                                    <  SRR19973066.1926924/150‑1 (MQ=60)
                                                 AGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGA                                                                                                    >  SRR19973066.165357/1‑150 (MQ=60)
                                                 AGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGA                                                                                                    >  SRR19973066.164993/1‑150 (MQ=60)
                                                    TCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTG                                                                                                 >  SRR19973066.3494232/1‑150 (MQ=60)
                                                         ATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGAC                                                                                            >  SRR19973066.1871089/1‑150 (MQ=60)
                                                         ATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGAC                                                                                            <  SRR19973066.3387415/150‑1 (MQ=60)
                                                         ATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGAC                                                                                            <  SRR19973066.3387237/150‑1 (MQ=60)
                                                           CCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCG                                                                                          >  SRR19973066.1462791/1‑150 (MQ=60)
                                                           CCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCG                                                                                          >  SRR19973066.1462795/1‑150 (MQ=60)
                                                               CGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAAT                                                                                      <  SRR19973066.2253923/150‑1 (MQ=60)
                                                                     GGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTG                                                                                <  SRR19973066.3434993/150‑1 (MQ=60)
                                                                        GCGCACTTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGT                                                                             <  SRR19973066.1775797/150‑1 (MQ=60)
                                                                          GCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGAAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCA                                                                           <  SRR19973066.286317/150‑1 (MQ=60)
                                                                                  CTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCC                                                                   >  SRR19973066.408627/1‑150 (MQ=60)
                                                                                      TGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCA                                                               <  SRR19973066.1772264/150‑1 (MQ=60)
                                                                                      TGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCA                                                               <  SRR19973066.1747755/150‑1 (MQ=60)
                                                                                       GCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAG                                                              >  SRR19973066.2199838/1‑150 (MQ=60)
                                                                                       GCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAG                                                              >  SRR19973066.2199859/1‑150 (MQ=60)
                                                                                            GGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAC                                                         <  SRR19973066.3445827/150‑1 (MQ=60)
                                                                                             GCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAACTCGCCTGTGTCAAAGGTGCCGCCAGCATACA                                                        <  SRR19973066.408905/150‑1 (MQ=60)
                                                                                             GCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACA                                                        >  SRR19973066.23886/1‑150 (MQ=60)
                                                                                             GCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACA                                                        >  SRR19973066.1841538/1‑150 (MQ=60)
                                                                                             GCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACA                                                        >  SRR19973066.209918/1‑150 (MQ=60)
                                                                                                ATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAC                                                     >  SRR19973066.956830/1‑150 (MQ=60)
                                                                                                ATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAC                                                     >  SRR19973066.12378/1‑150 (MQ=60)
                                                                                                 TCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACC                                                    <  SRR19973066.893110/150‑1 (MQ=60)
                                                                                                      AGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGT                                               <  SRR19973066.156896/150‑1 (MQ=60)
                                                                                                        CACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCA                                             <  SRR19973066.948730/150‑1 (MQ=60)
                                                                                                        CACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCA                                             <  SRR19973066.1327063/150‑1 (MQ=60)
                                                                                                          CTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGC                                           >  SRR19973066.2831555/1‑150 (MQ=60)
                                                                                                          CTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGC                                           <  SRR19973066.3162817/150‑1 (MQ=60)
                                                                                                           TGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCA                                          >  SRR19973066.2486417/1‑150 (MQ=60)
                                                                                                            GATGGCAATACGGTATATCCGTGCTCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAG                                         <  SRR19973066.1744807/150‑1 (MQ=60)
                                                                                                            GATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAG                                         <  SRR19973066.3438177/150‑1 (MQ=60)
                                                                                                                 CAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCT                                    <  SRR19973066.1076484/150‑1 (MQ=60)
                                                                                                                  AATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTT                                   <  SRR19973066.2953617/150‑1 (MQ=60)
                                                                                                                  AATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTT                                   >  SRR19973066.1477431/1‑150 (MQ=60)
                                                                                                                    TACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAG                                 <  SRR19973066.1563843/150‑1 (MQ=60)
                                                                                                                    TACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAG                                 >  SRR19973066.1515743/1‑150 (MQ=60)
                                                                                                                       GGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTA                              >  SRR19973066.1344209/1‑150 (MQ=60)
                                                                                                                            ATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTT                         >  SRR19973066.1206781/1‑150 (MQ=60)
                                                                                                                            ATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTT                         >  SRR19973066.3012878/1‑150 (MQ=60)
                                                                                                                             TCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTA                        <  SRR19973066.565890/150‑1 (MQ=60)
                                                                                                                             TCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTA                        <  SRR19973066.3330758/150‑1 (MQ=60)
                                                                                                                             TCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGCGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTA                        <  SRR19973066.564483/150‑1 (MQ=60)
                                                                                                                              CCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTAC                       <  SRR19973066.2604382/150‑1 (MQ=60)
                                                                                                                                    GCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACG                 <  SRR19973066.3048150/150‑1 (MQ=60)
                                                                                                                                    GCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACG                 >  SRR19973066.2789862/1‑150 (MQ=60)
                                                                                                                                     CTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGT                >  SRR19973066.3310335/1‑150 (MQ=60)
                                                                                                                                      TTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTC               <  SRR19973066.468870/150‑1 (MQ=60)
                                                                                                                                              TTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAC       <  SRR19973066.2250635/150‑1 (MQ=60)
                                                                                                                                                 TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    <  SRR19973066.722415/150‑1 (MQ=60)
                                                                                                                                                 TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    <  SRR19973066.447500/150‑1 (MQ=60)
                                                                                                                                                 TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    <  SRR19973066.2843396/150‑1 (MQ=60)
                                                                                                                                                 TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    <  SRR19973066.115386/150‑1 (MQ=60)
                                                                                                                                                  TAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGA   >  SRR19973066.2081152/1‑150 (MQ=60)
                                                                                                                                                   AACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  SRR19973066.1064013/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  CP009273/300623‑300919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑