Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 300,770 C→A 100% V49V (GTG→GTT)  ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273300,7700CA98.5% 860.3 / 2.2 263V49V (GTG→GTT) ecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (133/126);  total (137/126)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 264 total aligned reads displayed.

ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACAT  >  CP009273/300623‑300916
                                                                                                                                                   |                                                                                                                                                  
 tACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACt                                                                                                                                                 >  1:1963421/1‑150 (MQ=255)
  aCTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTa                                                                                                                                                >  1:1185294/1‑150 (MQ=255)
   cTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTac                                                                                                                                               >  2:3848299/1‑150 (MQ=255)
    tCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                              >  2:4192416/1‑150 (MQ=255)
     cGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTacac                                                                                                                                             <  2:4664557/150‑1 (MQ=255)
       ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                           <  2:2350526/150‑1 (MQ=255)
       ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                           >  1:3637629/1‑150 (MQ=255)
        tctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGtt                                                                                                                                          >  2:1358134/1‑150 (MQ=255)
         ctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGttt                                                                                                                                         <  2:961708/150‑1 (MQ=255)
          tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTg                                                                                                                                        >  1:3833472/1‑150 (MQ=255)
                   tGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAag                                                                                                                               >  1:737785/1‑150 (MQ=255)
                      aaCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtca                                                                                                                            >  1:1631814/1‑150 (MQ=255)
                      aaCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtca                                                                                                                            >  1:1280955/1‑150 (MQ=255)
                      aaCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtca                                                                                                                            >  2:194683/1‑150 (MQ=255)
                         gCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagtc                                                                                                                         <  2:2422037/150‑1 (MQ=255)
                           cAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtctc                                                                                                                       >  1:5041219/1‑150 (MQ=255)
                            aGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCg                                                                                                                      >  1:1133455/1‑150 (MQ=255)
                              cTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                    <  2:752310/150‑1 (MQ=255)
                              cTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                    <  2:4673508/150‑1 (MQ=255)
                               tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                   >  2:1600608/1‑150 (MQ=255)
                               tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                   <  2:1300330/150‑1 (MQ=255)
                               tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                   >  1:448004/1‑150 (MQ=255)
                               tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAc                                                                                                                   >  1:359947/1‑150 (MQ=255)
                                ggCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCATTACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACg                                                                                                                  <  2:4352762/150‑1 (MQ=255)
                                    gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAg                                                                                                              <  1:888983/150‑1 (MQ=255)
                                    gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAg                                                                                                              <  1:3076077/150‑1 (MQ=255)
                                      gggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCg                                                                                                            <  1:770146/150‑1 (MQ=255)
                                      gggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCg                                                                                                            <  1:4181094/150‑1 (MQ=255)
                                       ggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGa                                                                                                           <  2:2799407/150‑1 (MQ=255)
                                        gCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAAGTCACTGCTGCCCGGGCGATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaa                                                                                                          <  2:1005950/150‑1 (MQ=255)
                                          gAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaaaa                                                                                                        >  2:1032477/1‑150 (MQ=255)
                                          gAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaaaa                                                                                                        >  1:5037384/1‑150 (MQ=255)
                                            aaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                      <  1:3925701/150‑1 (MQ=255)
                                            aaaGCAGTACACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATCGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                      <  1:4392244/150‑1 (MQ=255)
                                                cAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAg                                                                                                  <  2:5019482/150‑1 (MQ=255)
                                                 aGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGa                                                                                                 >  1:3360502/1‑150 (MQ=255)
                                                  gTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGAt                                                                                                <  2:1161556/150‑1 (MQ=255)
                                                       ccATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGcca                                                                                           <  1:5132037/150‑2 (MQ=255)
                                                           ccACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccg                                                                                       <  2:3974019/150‑1 (MQ=255)
                                                            cACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccga                                                                                      >  1:1838870/1‑150 (MQ=255)
                                                            cACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccga                                                                                      >  1:2395494/1‑150 (MQ=255)
                                                            cACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccg                                                                                       <  1:2449953/149‑1 (MQ=255)
                                                             aCCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGaa                                                                                     >  2:1479555/1‑150 (MQ=255)
                                                              ccGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTGTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGaaa                                                                                    <  2:1848932/150‑1 (MQ=255)
                                                                ggTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATc                                                                                  >  1:4429879/1‑150 (MQ=255)
                                                                ggTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATc                                                                                  >  2:1860278/1‑150 (MQ=255)
                                                                ggTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATc                                                                                  >  2:1186572/1‑150 (MQ=255)
                                                                    gggTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCt                                                                              >  1:410087/1‑150 (MQ=255)
                                                                    gggTGCGCAATTCACGGCTGCCCGGGCTATCAATCGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCt                                                                              <  1:2940927/150‑1 (MQ=255)
                                                                        gcgcAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTgtgt                                                                          <  2:3422524/150‑1 (MQ=255)
                                                                         cgcAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTc                                                                         <  2:267805/150‑1 (MQ=255)
                                                                            aaTTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaaa                                                                      <  1:1618072/150‑1 (MQ=255)
                                                                            aaTTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaaa                                                                      >  1:3757205/1‑150 (MQ=255)
                                                                                 aCTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgc                                                                 >  2:936523/1‑150 (MQ=255)
                                                                                  ctgctgCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgcc                                                                >  2:4360332/1‑150 (MQ=255)
                                                                                   tgctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccg                                                               >  2:947128/1‑150 (MQ=255)
                                                                                   tgctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccg                                                               >  2:1380527/1‑150 (MQ=255)
                                                                                   tgctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccg                                                               >  2:791886/1‑150 (MQ=255)
                                                                                    gctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccgc                                                              >  1:318356/1‑150 (MQ=255)
                                                                                      tgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCa                                                            <  1:4941370/150‑1 (MQ=255)
                                                                                        cccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGCTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGc                                                          >  2:2839313/1‑150 (MQ=255)
                                                                                         ccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCa                                                         <  1:1104092/150‑1 (MQ=255)
                                                                                           gggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATa                                                       <  2:3666762/150‑1 (MQ=255)
                                                                                            ggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAc                                                      >  1:4642895/1‑150 (MQ=255)
                                                                                            ggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAc                                                      <  1:4806108/150‑1 (MQ=255)
                                                                                              cTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAg                                                    <  1:3143899/150‑1 (MQ=255)
                                                                                               tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGa                                                   >  1:1718694/1‑150 (MQ=255)
                                                                                               tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGa                                                   >  2:3093033/1‑150 (MQ=255)
                                                                                               tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGa                                                   >  1:1900708/1‑150 (MQ=255)
                                                                                                 tCAAGAGCACTGATGGCAATACGGTATAGCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAcc                                                 <  2:4754548/150‑1 (MQ=255)
                                                                                                    aTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCt                                              <  2:3546679/150‑1 (MQ=255)
                                                                                                     tAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTg                                             >  2:2336088/1‑150 (MQ=255)
                                                                                                         aCTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcag                                         >  2:54071/1‑150 (MQ=255)
                                                                                                         aCTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcag                                         <  1:2481094/150‑1 (MQ=255)
                                                                                                         aCTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcag                                         >  1:1982997/1‑150 (MQ=255)
                                                                                                         aCTGATGGCAATACGGTATATCCGTGCGCGTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAACTCGCCTGTGTCACAGGTGCCGCCAGCATACAGACCGCTGTcag                                         >  2:2626729/1‑150 (MQ=255)
                                                                                                           tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagca                                       >  2:2521342/1‑150 (MQ=255)
                                                                                                            gATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagcag                                      <  1:311158/150‑1 (MQ=255)
                                                                                                            gATGGCAATACGGTATATCCGTGCGCTTGTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagcag                                      >  2:4735903/1‑150 (MQ=255)
                                                                                                                  aacccGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGNTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCtt                                <  1:2679570/146‑1 (MQ=255)
                                                                                                                  aaTACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCTGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCtt                                <  2:1963421/150‑1 (MQ=255)
                                                                                                                     aCGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGt                             <  2:2903057/150‑1 (MQ=255)
                                                                                                                     aCGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGt                             <  2:373746/150‑1 (MQ=255)
                                                                                                                      cGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGtt                            <  1:1441199/150‑1 (MQ=255)
                                                                                                                       ggTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTa                           >  1:2792332/1‑150 (MQ=255)
                                                                                                                        gTATATCCGTGCGCGTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTaa                          >  2:461065/1‑150 (MQ=255)
                                                                                                                         tataTCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAAt                         <  1:1041738/150‑1 (MQ=255)
                                                                                                                           tatCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGt                       <  2:1661393/150‑1 (MQ=255)
                                                                                                                            atCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                      >  2:3008713/1‑150 (MQ=255)
                                                                                                                            atCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                      <  2:193267/150‑1 (MQ=255)
                                                                                                                                    gcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCAca              <  2:113020/150‑2 (MQ=255)
                                                                                                                                    gcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACg              >  2:3335209/1‑150 (MQ=255)
                                                                                                                                       tttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCg           <  2:4900164/150‑1 (MQ=255)
                                                                                                                                       tttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCg           <  2:2615652/150‑1 (MQ=255)
                                                                                                                                       tttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCg           <  2:663873/150‑1 (MQ=255)
                                                                                                                                         tgttgtTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGcc         >  2:2120519/1‑150 (MQ=255)
                                                                                                                                          gttgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCt        <  1:2629067/150‑1 (MQ=255)
                                                                                                                                              ttattaACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAAc    >  2:4784971/1‑150 (MQ=255)
                                                                                                                                               tATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACa   >  2:3205741/1‑150 (MQ=255)
                                                                                                                                                aTTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACAt  <  2:22800/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACAT  >  CP009273/300623‑300916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  CP009273/300622‑300919
                                                                                                                                                    |                                                                                                                                                     
AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAA                                                                                                                                                      <  SRR19973063.1893221/150‑1 (MQ=60)
 ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAC                                                                                                                                                     >  SRR19973063.4586931/1‑150 (MQ=60)
 ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAC                                                                                                                                                     >  SRR19973063.4915984/1‑150 (MQ=60)
  TACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACT                                                                                                                                                    >  SRR19973063.1969543/1‑150 (MQ=60)
  TACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACT                                                                                                                                                    >  SRR19973063.1969567/1‑150 (MQ=60)
  TACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACT                                                                                                                                                    >  SRR19973063.2892727/1‑150 (MQ=60)
  TACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACT                                                                                                                                                    >  SRR19973063.236935/1‑150 (MQ=60)
   ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTA                                                                                                                                                   >  SRR19973063.1188790/1‑150 (MQ=60)
   ACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTA                                                                                                                                                   >  SRR19973063.1188861/1‑150 (MQ=60)
     TCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAAAACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACA                                                                                                                                                 >  SRR19973063.1961928/1‑150 (MQ=60)
       GCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACG                                                                                                                                               <  SRR19973063.1132599/150‑1 (MQ=60)
        CTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGT                                                                                                                                              >  SRR19973063.3649300/1‑150 (MQ=60)
         TCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTT                                                                                                                                             <  SRR19973063.763366/150‑1 (MQ=60)
          CTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTT                                                                                                                                            >  SRR19973063.1086700/1‑150 (MQ=60)
           TCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTG                                                                                                                                           >  SRR19973063.2784535/1‑150 (MQ=60)
           TCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTG                                                                                                                                           >  SRR19973063.3845735/1‑150 (MQ=60)
           TCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTG                                                                                                                                           >  SRR19973063.3845666/1‑150 (MQ=60)
                AGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGAC                                                                                                                                      <  SRR19973063.1115238/150‑1 (MQ=60)
                AGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGAC                                                                                                                                      >  SRR19973063.3687496/1‑150 (MQ=60)
                    TGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAG                                                                                                                                  >  SRR19973063.739948/1‑150 (MQ=60)
                    TGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAG                                                                                                                                  >  SRR19973063.1272118/1‑150 (MQ=60)
                     GAAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGT                                                                                                                                 <  SRR19973063.2667120/150‑1 (MQ=60)
                      CAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTC                                                                                                                                >  SRR19973063.4812525/1‑150 (MQ=60)
                       AACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCA                                                                                                                               >  SRR19973063.1284775/1‑150 (MQ=60)
                       AACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCA                                                                                                                               >  SRR19973063.1636799/1‑150 (MQ=60)
                          GCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTC                                                                                                                            >  SRR19973063.3114760/1‑150 (MQ=60)
                          GCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTC                                                                                                                            >  SRR19973063.4437720/1‑150 (MQ=60)
                           CCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCT                                                                                                                           >  SRR19973063.4182400/1‑150 (MQ=60)
                            CAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTC                                                                                                                          >  SRR19973063.3947724/1‑150 (MQ=60)
                            CAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTC                                                                                                                          >  SRR19973063.5057463/1‑150 (MQ=60)
                            CAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTC                                                                                                                          >  SRR19973063.5057464/1‑150 (MQ=60)
                            CAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTC                                                                                                                          >  SRR19973063.5166145/1‑150 (MQ=60)
                             AGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCG                                                                                                                         <  SRR19973063.4454360/150‑1 (MQ=60)
                             AGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCG                                                                                                                         >  SRR19973063.1136892/1‑150 (MQ=60)
                                TGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAC                                                                                                                      >  SRR19973063.360985/1‑150 (MQ=60)
                                TGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAC                                                                                                                      >  SRR19973063.449267/1‑150 (MQ=60)
                                TGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGAC                                                                                                                      >  SRR19973063.360948/1‑150 (MQ=60)
                                 GGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACG                                                                                                                     >  SRR19973063.2113473/1‑150 (MQ=60)
                                    GGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCA                                                                                                                  <  SRR19973063.2552626/150‑1 (MQ=60)
                                     GGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAG                                                                                                                 <  SRR19973063.3085960/150‑1 (MQ=60)
                                     GGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAG                                                                                                                 <  SRR19973063.891554/150‑1 (MQ=60)
                                      GGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGC                                                                                                                <  SRR19973063.957087/150‑1 (MQ=60)
                                       GGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCG                                                                                                               <  SRR19973063.4194446/150‑1 (MQ=60)
                                       GGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCG                                                                                                               <  SRR19973063.772390/150‑1 (MQ=60)
                                        GGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGA                                                                                                              <  SRR19973063.3823671/150‑1 (MQ=60)
                                        GGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGA                                                                                                              <  SRR19973063.5001643/150‑1 (MQ=60)
                                           GAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAA                                                                                                           >  SRR19973063.5053619/1‑150 (MQ=60)
                                           GAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAA                                                                                                           >  SRR19973063.3913212/1‑150 (MQ=60)
                                             AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973063.3938231/150‑1 (MQ=60)
                                             AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973063.4406356/150‑1 (MQ=60)
                                             AAAGCAGTACACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATCGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973063.4406308/150‑1 (MQ=60)
                                                GCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCA                                                                                                      <  SRR19973063.945013/150‑1 (MQ=60)
                                                  AGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGA                                                                                                    >  SRR19973063.3371203/1‑150 (MQ=60)
                                                        CCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCA                                                                                              <  SRR19973063.5148536/150‑1 (MQ=60)
                                                        CCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCA                                                                                              <  SRR19973063.5148545/150‑1 (MQ=60)
                                                            TCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCG                                                                                          <  SRR19973063.2457778/150‑1 (MQ=60)
                                                            CCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCG                                                                                          >  SRR19973063.5132172/1‑150 (MQ=60)
                                                             CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGA                                                                                         >  SRR19973063.1844692/1‑150 (MQ=60)
                                                             CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGA                                                                                         >  SRR19973063.1844665/1‑150 (MQ=60)
                                                             CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGA                                                                                         >  SRR19973063.2403159/1‑150 (MQ=60)
                                                             CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGA                                                                                         >  SRR19973063.1986158/1‑150 (MQ=60)
                                                             CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGA                                                                                         >  SRR19973063.1844607/1‑150 (MQ=60)
                                                             CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGA                                                                                         >  SRR19973063.84152/1‑150 (MQ=60)
                                                             CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATAAAATACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAAAGTCAGATTGCCGACCGA                                                                                         >  SRR19973063.1844552/1‑150 (MQ=60)
                                                              ACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAA                                                                                        >  SRR19973063.1648242/1‑150 (MQ=60)
                                                                 GGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATC                                                                                     >  SRR19973063.4444094/1‑150 (MQ=60)
                                                                  GTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCG                                                                                    >  SRR19973063.314314/1‑150 (MQ=60)
                                                                     GGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCT                                                                                 >  SRR19973063.411243/1‑150 (MQ=60)
                                                                     GGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCT                                                                                 >  SRR19973063.2416778/1‑150 (MQ=60)
                                                                     GGGTGCGCAATTCACGGCTGCCCGGGCTATCAATCGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCT                                                                                 <  SRR19973063.2950318/150‑1 (MQ=60)
                                                                       GTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGT                                                                               <  SRR19973063.1362215/150‑1 (MQ=60)
                                                                        TGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTG                                                                              <  SRR19973063.1438283/150‑1 (MQ=60)
                                                                        TGCGCAATTCACTGCTGCCCGGGCTATCAAGAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTG                                                                              <  SRR19973063.4258877/150‑1 (MQ=60)
                                                                            CAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAA                                                                          >  SRR19973063.1666407/1‑150 (MQ=60)
                                                                             AATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAA                                                                         <  SRR19973063.1623020/150‑1 (MQ=60)
                                                                             AATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAA                                                                         <  SRR19973063.1623021/150‑1 (MQ=60)
                                                                             AATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAA                                                                         >  SRR19973063.3769204/1‑150 (MQ=60)
                                                                              ATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAG                                                                        <  SRR19973063.2725155/150‑1 (MQ=60)
                                                                               TTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGG                                                                       >  SRR19973063.1633870/1‑150 (MQ=60)
                                                                                TCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGT                                                                      >  SRR19973063.3448947/1‑150 (MQ=60)
                                                                                 CACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTG                                                                     >  SRR19973063.3212757/1‑150 (MQ=60)
                                                                                     GCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGC                                                                 >  SRR19973063.319288/1‑150 (MQ=60)
                                                                                       TGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCA                                                               <  SRR19973063.4957347/150‑1 (MQ=60)
                                                                                       TGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCA                                                               <  SRR19973063.4957442/150‑1 (MQ=60)
                                                                                          CCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCA                                                            <  SRR19973063.1107454/150‑1 (MQ=60)
                                                                                           CGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAT                                                           >  SRR19973063.2459973/1‑150 (MQ=60)
                                                                                           CGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAT                                                           >  SRR19973063.2461283/1‑150 (MQ=60)
                                                                                             GGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAC                                                         <  SRR19973063.4821473/150‑1 (MQ=60)
                                                                                             GGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAC                                                         >  SRR19973063.4657727/1‑150 (MQ=60)
                                                                                              GCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACA                                                        <  SRR19973063.3164862/150‑1 (MQ=60)
                                                                                              GCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTGGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACA                                                        <  SRR19973063.2848777/150‑1 (MQ=60)
                                                                                               CTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAG                                                       <  SRR19973063.3154007/150‑1 (MQ=60)
                                                                                                TATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGA                                                      >  SRR19973063.1723938/1‑150 (MQ=60)
                                                                                                TATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGA                                                      >  SRR19973063.1906654/1‑150 (MQ=60)
                                                                                                  TCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACC                                                    <  SRR19973063.4055839/150‑1 (MQ=60)
                                                                                                   CAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCG                                                   >  SRR19973063.586286/1‑150 (MQ=60)
                                                                                                    AATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGC                                                  >  SRR19973063.3289688/1‑150 (MQ=60)
                                                                                                        GCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTC                                              <  SRR19973063.3096050/150‑1 (MQ=60)
                                                                                                        GCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTC                                              >  SRR19973063.455352/1‑150 (MQ=60)
                                                                                                          ACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAG                                            <  SRR19973063.2489023/150‑1 (MQ=60)
                                                                                                          ACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAG                                            >  SRR19973063.1989196/1‑150 (MQ=60)
                                                                                                             GATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAG                                         <  SRR19973063.312070/150‑1 (MQ=60)
                                                                                                             GATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAG                                         <  SRR19973063.312069/150‑1 (MQ=60)
                                                                                                                GGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGC                                      <  SRR19973063.3757102/150‑1 (MQ=60)
                                                                                                                   aaccCGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGNTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTT                                   <  SRR19973063.2688196/146‑1 (MQ=60)
                                                                                                                   AATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTT                                   >  SRR19973063.472126/1‑150 (MQ=60)
                                                                                                                    ATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTA                                  <  SRR19973063.3629449/150‑1 (MQ=60)
                                                                                                                     TACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAG                                 <  SRR19973063.4231364/150‑1 (MQ=60)
                                                                                                                       CGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTT                               <  SRR19973063.1445599/150‑1 (MQ=60)
                                                                                                                        GGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTA                              >  SRR19973063.2801315/1‑150 (MQ=60)
                                                                                                                          TATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAAT                            <  SRR19973063.1044892/150‑1 (MQ=60)
                                                                                                                           ATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATG                           >  SRR19973063.1202070/1‑150 (MQ=60)
                                                                                                                           ATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATG                           <  SRR19973063.808739/150‑1 (MQ=60)
                                                                                                                            TATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGT                          >  SRR19973063.15455/1‑150 (MQ=60)
                                                                                                                             ATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTT                         <  SRR19973063.2556570/150‑1 (MQ=60)
                                                                                                                                    CGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCAC                  >  SRR19973063.2998920/1‑150 (MQ=60)
                                                                                                                                           GTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCT           <  SRR19973063.2637452/150‑1 (MQ=60)
                                                                                                                                                  TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    <  SRR19973063.2393722/150‑1 (MQ=60)
                                                                                                                                                  TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    <  SRR19973063.1342564/150‑1 (MQ=60)
                                                                                                                                                  TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    <  SRR19973063.1872808/150‑1 (MQ=60)
                                                                                                                                                    AACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  SRR19973063.3776607/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  CP009273/300622‑300919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑