Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 300,770 C→A 91.3% V49V (GTG→GTT)  ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273300,7700CA91.3% 677.4 / 55.4 242V49V (GTG→GTT) ecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (10/11);  new base A (111/110);  total (121/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 243 total aligned reads displayed.

ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGA  >  CP009273/300623‑300918
                                                                                                                                                   |                                                                                                                                                    
aTACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCa                                                                                                                                                     >  1:484536/1‑149 (MQ=255)
 tACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCAc                                                                                                                                                    >  1:2476773/1‑149 (MQ=255)
     cGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTaca                                                                                                                                                <  1:598547/149‑1 (MQ=255)
       ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACg                                                                                                                                              <  1:1067493/149‑1 (MQ=255)
       ctctCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACg                                                                                                                                              >  1:3741986/1‑149 (MQ=255)
        tctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                             >  2:506442/1‑149 (MQ=255)
        tctcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGt                                                                                                                                             >  1:2749793/1‑149 (MQ=255)
          tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGttt                                                                                                                                           >  1:3274401/1‑149 (MQ=255)
          tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGttt                                                                                                                                           <  2:1206391/149‑1 (MQ=255)
          tcACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGttt                                                                                                                                           >  2:2076020/1‑149 (MQ=255)
           cACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTg                                                                                                                                          <  1:657784/149‑1 (MQ=255)
            aCCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGc                                                                                                                                         >  2:4116360/1‑149 (MQ=255)
            aCCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGGTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCACTAGCACTGATGGCAATACGGTATATCCGTGCGCTGTTGTTGTTATTAACTACACGTTTGc                                                                                                                                         <  2:2316335/149‑1 (MQ=255)
             ccAGCCTGCAACGCCAGCTGGTGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCt                                                                                                                                        <  2:1716467/149‑1 (MQ=255)
              cAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTg                                                                                                                                       <  1:3010226/149‑1 (MQ=255)
                  cTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACaa                                                                                                                                   >  2:1005705/1‑149 (MQ=255)
                      aaCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAagtc                                                                                                                               >  1:234777/1‑149 (MQ=255)
                       aCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtca                                                                                                                              <  2:1474562/149‑1 (MQ=255)
                       aCGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtca                                                                                                                              <  2:2480421/149‑1 (MQ=255)
                         gCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagt                                                                                                                            >  1:2310572/1‑149 (MQ=255)
                         gCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagt                                                                                                                            >  1:2310350/1‑149 (MQ=255)
                          ccAGCTGTCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagtc                                                                                                                           >  1:343205/1‑149 (MQ=255)
                          ccAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagtc                                                                                                                           >  1:2546057/1‑149 (MQ=255)
                          ccAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAagtcagtc                                                                                                                           >  1:3810636/1‑149 (MQ=255)
                           cAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtct                                                                                                                          <  1:643519/149‑1 (MQ=255)
                            aGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtctc                                                                                                                         <  1:3372658/149‑1 (MQ=255)
                            aGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGtctc                                                                                                                         <  1:862281/149‑1 (MQ=255)
                               tGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGa                                                                                                                      >  2:2587046/1‑149 (MQ=255)
                                    gggggCGAACAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATCGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCa                                                                                                                 <  2:4579376/149‑1 (MQ=255)
                                    gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTTCTGACAAAGTCAGTCTCGGACGGCa                                                                                                                 >  2:283940/1‑149 (MQ=255)
                                    gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCa                                                                                                                 >  2:2677390/1‑149 (MQ=255)
                                    gggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCa                                                                                                                 >  1:2488935/1‑149 (MQ=255)
                                     ggggCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAg                                                                                                                <  1:2169165/149‑1 (MQ=255)
                                          gAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGaaa                                                                                                           <  1:2076020/149‑1 (MQ=255)
                                            aaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAAt                                                                                                         <  1:4124115/149‑1 (MQ=255)
                                            aaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAAt                                                                                                         <  1:4298871/149‑1 (MQ=255)
                                            aaaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTAAACGTTTTCTGACAAAGTCAGTCTCGGAAGGCAGCGAAAAt                                                                                                         >  2:4156881/1‑149 (MQ=255)
                                            aaaGCAGTTCACCATACACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGATATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAAt                                                                                                         <  1:3921003/149‑1 (MQ=255)
                                             aaGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATg                                                                                                        >  1:2185270/1‑149 (MQ=255)
                                               gCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTc                                                                                                      <  2:3963810/149‑1 (MQ=255)
                                                 aGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAg                                                                                                    >  2:3765591/1‑149 (MQ=255)
                                                  gTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGa                                                                                                   <  1:4445698/149‑1 (MQ=255)
                                                   ttCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGAt                                                                                                  >  1:303850/1‑149 (MQ=255)
                                                    tCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGAtt                                                                                                 <  2:4784253/149‑1 (MQ=255)
                                                      aCCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGc                                                                                               <  1:3522717/149‑1 (MQ=255)
                                                      aCCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGc                                                                                               >  1:4613580/1‑149 (MQ=255)
                                                      aCCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGc                                                                                               >  1:1284665/1‑149 (MQ=255)
                                                          tCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgac                                                                                           >  2:2595716/1‑149 (MQ=255)
                                                           ccACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgacc                                                                                          <  2:3845460/149‑1 (MQ=255)
                                                            cACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccg                                                                                         >  1:4083283/1‑149 (MQ=255)
                                                             aCCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccga                                                                                        >  1:1964340/1‑149 (MQ=255)
                                                             aCCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGccgaccga                                                                                        >  2:1962425/1‑149 (MQ=255)
                                                               cGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGaaa                                                                                      <  1:948291/149‑1 (MQ=255)
                                                                ggTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAAt                                                                                     <  1:3014572/149‑1 (MQ=255)
                                                                       tGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCtgt                                                                              <  1:1167878/149‑1 (MQ=255)
                                                                           cAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCa                                                                          >  2:3492563/1‑149 (MQ=255)
                                                                             aTTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCaaa                                                                        >  1:914976/1‑149 (MQ=255)
                                                                                cACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGt                                                                     <  1:1924627/149‑1 (MQ=255)
                                                                                     ctgcCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTgccgc                                                                <  1:2280048/149‑1 (MQ=255)
                                                                                        cccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAg                                                             >  2:4646080/1‑149 (MQ=255)
                                                                                           gggCTATCAATCGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAt                                                          <  2:132886/149‑1 (MQ=255)
                                                                                           gggCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCAt                                                          <  1:166633/149‑1 (MQ=255)
                                                                                               tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAg                                                      >  2:3359774/1‑149 (MQ=255)
                                                                                                aTCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGa                                                     <  2:2999232/149‑1 (MQ=255)
                                                                                                 tCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                    >  1:4066820/1‑149 (MQ=255)
                                                                                                 tCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAc                                                    <  1:4578361/149‑1 (MQ=255)
                                                                                                   aaTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCg                                                  >  2:3823526/1‑149 (MQ=255)
                                                                                                      aGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTg                                               >  2:860030/1‑149 (MQ=255)
                                                                                                       gCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGt                                              <  2:3148470/149‑1 (MQ=255)
                                                                                                        cACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTc                                             <  1:2695203/149‑1 (MQ=255)
                                                                                                           tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagc                                          >  1:564269/1‑149 (MQ=255)
                                                                                                           tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagc                                          >  1:564392/1‑149 (MQ=255)
                                                                                                           tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagc                                          >  2:1608857/1‑149 (MQ=255)
                                                                                                            gATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagca                                         <  2:308178/149‑1 (MQ=255)
                                                                                                             aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagcag                                        >  2:2608546/1‑149 (MQ=255)
                                                                                                             aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagcag                                        >  1:321453/1‑149 (MQ=255)
                                                                                                             aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagcag                                        >  2:423365/1‑149 (MQ=255)
                                                                                                             aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTcagcag                                        >  1:4769758/1‑149 (MQ=255)
                                                                                                              tGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAgg                                       >  2:2353371/1‑149 (MQ=255)
                                                                                                              tGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAgg                                       >  1:4531942/1‑149 (MQ=255)
                                                                                                                 cAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGcc                                    <  2:1713985/149‑1 (MQ=255)
                                                                                                                 cAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGcc                                    <  1:4206853/149‑1 (MQ=255)
                                                                                                                 cAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCACTGTCAGCAGGGcc                                    <  2:4536831/149‑1 (MQ=255)
                                                                                                                  aaTACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCt                                   <  2:343205/149‑1 (MQ=255)
                                                                                                                    tACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTa                                 <  2:3913272/149‑1 (MQ=255)
                                                                                                                    tACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTa                                 <  2:1259398/149‑1 (MQ=255)
                                                                                                                    tACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTa                                 >  2:1922552/1‑149 (MQ=255)
                                                                                                                    tACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTa                                 <  2:649351/149‑1 (MQ=255)
                                                                                                                        gTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTa                             >  1:956701/1‑149 (MQ=255)
                                                                                                                         tataTCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTaa                            <  2:4246659/149‑1 (MQ=255)
                                                                                                                             tCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGtt                        <  2:3274401/149‑1 (MQ=255)
                                                                                                                              ccGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTa                       <  1:3583017/149‑1 (MQ=255)
                                                                                                                               cGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTAc                      <  2:3805613/149‑1 (MQ=255)
                                                                                                                                  gcgcTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGcc                   >  1:1592971/1‑149 (MQ=255)
                                                                                                                                     cTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACg                >  2:2200205/1‑149 (MQ=255)
                                                                                                                                       tttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTc              <  2:2728855/149‑1 (MQ=255)
                                                                                                                                       tttGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTc              <  2:3637038/149‑1 (MQ=255)
                                                                                                                                          gttgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGcc           <  2:689974/149‑1 (MQ=255)
                                                                                                                                           ttgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCt          >  2:597794/1‑149 (MQ=255)
                                                                                                                                           ttgttATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCt          <  2:2749793/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGA  >  CP009273/300623‑300918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  CP009273/300621‑300919
                                                                                                                                                     |                                                                                                                                                     
AAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTA                                                                                                                                                       <  SRR19973071.1094641/150‑1 (MQ=60)
AAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTA                                                                                                                                                       <  SRR19973071.2391239/150‑1 (MQ=60)
AAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTA                                                                                                                                                       >  SRR19973071.2076839/1‑150 (MQ=60)
AAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTA                                                                                                                                                       >  SRR19973071.2076696/1‑150 (MQ=60)
AAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTA                                                                                                                                                       <  SRR19973071.1884057/150‑1 (MQ=60)
 AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCA                                                                                                                                                      >  SRR19973071.486322/1‑150 (MQ=60)
 AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAA                                                                                                                                                      <  SRR19973071.2551628/150‑1 (MQ=60)
 AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAA                                                                                                                                                      >  SRR19973071.3439150/1‑150 (MQ=60)
 AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAA                                                                                                                                                      >  SRR19973071.1260866/1‑150 (MQ=60)
 AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAA                                                                                                                                                      >  SRR19973071.161976/1‑150 (MQ=60)
 AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAA                                                                                                                                                      >  SRR19973071.162052/1‑150 (MQ=60)
 AATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAA                                                                                                                                                      >  SRR19973071.3439051/1‑150 (MQ=60)
  ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCAC                                                                                                                                                     >  SRR19973071.2485784/1‑150 (MQ=60)
  ATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAAC                                                                                                                                                     <  SRR19973071.210649/150‑1 (MQ=60)
   TACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACT                                                                                                                                                    >  SRR19973071.921115/1‑150 (MQ=60)
     CTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTAC                                                                                                                                                  >  SRR19973071.1293505/1‑150 (MQ=60)
      TCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACA                                                                                                                                                 <  SRR19973071.1992457/150‑1 (MQ=60)
       CGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACAC                                                                                                                                                <  SRR19973071.600775/150‑1 (MQ=60)
        GCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACG                                                                                                                                               >  SRR19973071.3755261/1‑150 (MQ=60)
        GCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACG                                                                                                                                               <  SRR19973071.2298830/150‑1 (MQ=60)
         CTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGT                                                                                                                                              <  SRR19973071.1071304/150‑1 (MQ=60)
         CTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGT                                                                                                                                              >  SRR19973071.2759527/1‑150 (MQ=60)
          TCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTT                                                                                                                                             <  SRR19973071.2178998/150‑1 (MQ=60)
           CTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTT                                                                                                                                            >  SRR19973071.3285990/1‑150 (MQ=60)
             CACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGC                                                                                                                                          <  SRR19973071.660172/150‑1 (MQ=60)
              ACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCT                                                                                                                                         >  SRR19973071.332104/1‑150 (MQ=60)
                CAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGA                                                                                                                                       <  SRR19973071.3020971/150‑1 (MQ=60)
                 AGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGAC                                                                                                                                      >  SRR19973071.605606/1‑150 (MQ=60)
                 AGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGAC                                                                                                                                      >  SRR19973071.843226/1‑150 (MQ=60)
                 AGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGAC                                                                                                                                      >  SRR19973071.3240901/1‑150 (MQ=60)
                      GCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAAGTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGT                                                                                                                                 <  SRR19973071.3611339/150‑1 (MQ=60)
                       CAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTC                                                                                                                                >  SRR19973071.236207/1‑150 (MQ=60)
                       CAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTC                                                                                                                                >  SRR19973071.235683/1‑150 (MQ=60)
                          CGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGT                                                                                                                             >  SRR19973071.2318777/1‑150 (MQ=60)
                          CGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGT                                                                                                                             >  SRR19973071.2318999/1‑150 (MQ=60)
                          CGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGT                                                                                                                             >  SRR19973071.2318992/1‑150 (MQ=60)
                          CGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGT                                                                                                                             >  SRR19973071.2318953/1‑150 (MQ=60)
                           GCCAGCTGTCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTATGCTGACAAAGTCAGTC                                                                                                                            >  SRR19973071.344338/1‑150 (MQ=60)
                           GCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTC                                                                                                                            >  SRR19973071.2555315/1‑150 (MQ=60)
                           GCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTC                                                                                                                            >  SRR19973071.3824159/1‑150 (MQ=60)
                            CCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCT                                                                                                                           >  SRR19973071.3029206/1‑150 (MQ=60)
                            CCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCT                                                                                                                           >  SRR19973071.628586/1‑150 (MQ=60)
                             CAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTC                                                                                                                          <  SRR19973071.2570168/150‑1 (MQ=60)
                             CAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTC                                                                                                                          <  SRR19973071.645873/150‑1 (MQ=60)
                             CAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTC                                                                                                                          <  SRR19973071.645945/150‑1 (MQ=60)
                              AGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCG                                                                                                                         <  SRR19973071.865363/150‑1 (MQ=60)
                              AGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCG                                                                                                                         <  SRR19973071.3384625/150‑1 (MQ=60)
                                     GGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCA                                                                                                                  >  SRR19973071.2497992/1‑150 (MQ=60)
                                     GGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCA                                                                                                                  <  SRR19973071.3811758/150‑1 (MQ=60)
                                     GGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCA                                                                                                                  >  SRR19973071.2497968/1‑150 (MQ=60)
                                       GGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGC                                                                                                                <  SRR19973071.4583265/150‑1 (MQ=60)
                                       GGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGC                                                                                                                <  SRR19973071.2177191/150‑1 (MQ=60)
                                         GGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGA                                                                                                              >  SRR19973071.3572202/1‑150 (MQ=60)
                                          GCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCACTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAA                                                                                                             <  SRR19973071.2347376/150‑1 (MQ=60)
                                            GAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAA                                                                                                           <  SRR19973071.4489619/150‑1 (MQ=60)
                                            GAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAA                                                                                                           <  SRR19973071.2083666/150‑1 (MQ=60)
                                             AAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAAT                                                                                                          <  SRR19973071.2422207/150‑1 (MQ=60)
                                              AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973071.4138796/150‑1 (MQ=60)
                                              AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973071.4138773/150‑1 (MQ=60)
                                              AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973071.4314083/150‑1 (MQ=60)
                                              AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         >  SRR19973071.2193354/1‑150 (MQ=60)
                                              AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973071.3934746/150‑1 (MQ=60)
                                              AAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         >  SRR19973071.1132760/1‑150 (MQ=60)
                                              AAAGCAGTTCACCATACACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGATATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATG                                                                                                         <  SRR19973071.3934821/150‑1 (MQ=60)
                                                AGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTC                                                                                                       >  SRR19973071.4240335/1‑150 (MQ=60)
                                                  CAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAG                                                                                                     >  SRR19973071.4722177/1‑150 (MQ=60)
                                                    GTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGAT                                                                                                   >  SRR19973071.304919/1‑150 (MQ=60)
                                                    GTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGAT                                                                                                   <  SRR19973071.4461412/150‑1 (MQ=60)
                                                       CACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGC                                                                                                >  SRR19973071.1289333/1‑150 (MQ=60)
                                                       CACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGC                                                                                                >  SRR19973071.4630027/1‑150 (MQ=60)
                                                       CACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTGTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGC                                                                                                >  SRR19973071.4630001/1‑150 (MQ=60)
                                                        ACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCC                                                                                               <  SRR19973071.3535059/150‑1 (MQ=60)
                                                         CCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCG                                                                                              <  SRR19973071.507285/150‑1 (MQ=60)
                                                         CCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCG                                                                                              <  SRR19973071.255179/150‑1 (MQ=60)
                                                           ATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGAC                                                                                            >  SRR19973071.364308/1‑150 (MQ=60)
                                                             CCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCG                                                                                          >  SRR19973071.4097775/1‑150 (MQ=60)
                                                              CACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGA                                                                                         >  SRR19973071.1971469/1‑150 (MQ=60)
                                                                 CGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAAT                                                                                      <  SRR19973071.951733/150‑1 (MQ=60)
                                                                 CGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAAT                                                                                      >  SRR19973071.2776788/1‑150 (MQ=60)
                                                                  GGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATC                                                                                     <  SRR19973071.3025326/150‑1 (MQ=60)
                                                                  GGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATC                                                                                     <  SRR19973071.3024774/150‑1 (MQ=60)
                                                                       GGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTG                                                                                <  SRR19973071.432562/150‑1 (MQ=60)
                                                                        GTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGT                                                                               <  SRR19973071.4306124/150‑1 (MQ=60)
                                                                         TGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTG                                                                              <  SRR19973071.952124/150‑1 (MQ=60)
                                                                         TGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTG                                                                              <  SRR19973071.1171911/150‑1 (MQ=60)
                                                                          GCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGT                                                                             <  SRR19973071.1382722/150‑1 (MQ=60)
                                                                              AATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAA                                                                         >  SRR19973071.918286/1‑150 (MQ=60)
                                                                                 TAACTGCTGCCCGGGCTATAAATAGCACTGATGGCAATACTGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCTGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGT                                                                      <  SRR19973071.3036941/150‑1 (MQ=60)
                                                                                  CACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTG                                                                     <  SRR19973071.1931655/150‑1 (MQ=60)
                                                                                     TGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCG                                                                  <  SRR19973071.1750647/150‑1 (MQ=60)
                                                                                       CTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCC                                                                <  SRR19973071.2288418/150‑1 (MQ=60)
                                                                                       CTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCC                                                                <  SRR19973071.2288271/150‑1 (MQ=60)
                                                                                             GGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATA                                                          <  SRR19973071.167354/150‑1 (MQ=60)
                                                                                              GGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAC                                                         >  SRR19973071.908620/1‑150 (MQ=60)
                                                                                              GGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATAC                                                         <  SRR19973071.315800/150‑1 (MQ=60)
                                                                                                  ATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAC                                                     <  SRR19973071.2612296/150‑1 (MQ=60)
                                                                                                  ATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAC                                                     >  SRR19973071.4081221/1‑150 (MQ=60)
                                                                                                   TCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACC                                                    <  SRR19973071.4594682/150‑1 (MQ=60)
                                                                                                    CAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGATGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGAACG                                                   >  SRR19973071.2034103/1‑150 (MQ=60)
                                                                                                      ATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCT                                                 >  SRR19973071.3297447/1‑150 (MQ=60)
                                                                                                          CACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACTTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCA                                             <  SRR19973071.4095682/150‑1 (MQ=60)
                                                                                                          CACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCA                                             <  SRR19973071.2747042/150‑1 (MQ=60)
                                                                                                          CACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCA                                             <  SRR19973071.346872/150‑1 (MQ=60)
                                                                                                          CACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCA                                             <  SRR19973071.2704825/150‑1 (MQ=60)
                                                                                                           ACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAG                                            >  SRR19973071.949649/1‑150 (MQ=60)
                                                                                                           ACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAG                                            <  SRR19973071.4685/150‑1 (MQ=60)
                                                                                                            CTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGC                                           >  SRR19973071.3448273/1‑150 (MQ=60)
                                                                                                            CTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGC                                           >  SRR19973071.566510/1‑150 (MQ=60)
                                                                                                            CTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGC                                           >  SRR19973071.566466/1‑150 (MQ=60)
                                                                                                            CTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGC                                           >  SRR19973071.566387/1‑150 (MQ=60)
                                                                                                              GATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAG                                         >  SRR19973071.4786659/1‑150 (MQ=60)
                                                                                                              GATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAG                                         >  SRR19973071.4786603/1‑150 (MQ=60)
                                                                                                              GATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAG                                         >  SRR19973071.322552/1‑150 (MQ=60)
                                                                                                               ATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGG                                        >  SRR19973071.4023890/1‑150 (MQ=60)
                                                                                                               ATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGG                                        >  SRR19973071.4548083/1‑150 (MQ=60)
                                                                                                                  GCAAGACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCC                                     <  SRR19973071.589561/150‑1 (MQ=60)
                                                                                                                   CAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCT                                    <  SRR19973071.4221821/150‑1 (MQ=60)
                                                                                                                   CAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCT                                    >  SRR19973071.4103870/1‑150 (MQ=60)
                                                                                                                   CAATACGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCT                                    <  SRR19973071.2877526/150‑1 (MQ=60)
                                                                                                                     ATACGTTATATCCGGGCGCTTTTGTTGTTATTACCTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCTGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTA                                  <  SRR19973071.522414/150‑1 (MQ=60)
                                                                                                                        CGGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTT                               >  SRR19973071.221977/1‑150 (MQ=60)
                                                                                                                         GGTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTA                              >  SRR19973071.960176/1‑150 (MQ=60)
                                                                                                                          GTATATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAA                             >  SRR19973071.1752959/1‑150 (MQ=60)
                                                                                                                              ATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTT                         <  SRR19973071.4171685/150‑1 (MQ=60)
                                                                                                                              ATCCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTT                         >  SRR19973071.1608842/1‑150 (MQ=60)
                                                                                                                               tttctaagtctaaGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTA                        <  SRR19973071.2039376/137‑1 (MQ=60)
                                                                                                                                CCGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTAC                       <  SRR19973071.3595460/150‑1 (MQ=60)
                                                                                                                                 CGTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGAGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACG                      >  SRR19973071.4563195/1‑150 (MQ=60)
                                                                                                                                  GTGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGC                     >  SRR19973071.3388802/1‑150 (MQ=60)
                                                                                                                                   TGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCC                    >  SRR19973071.1598595/1‑150 (MQ=60)
                                                                                                                                   TGCGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCC                    <  SRR19973071.211818/150‑1 (MQ=60)
                                                                                                                                     CGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCAC                  >  SRR19973071.717410/1‑150 (MQ=60)
                                                                                                                                     CGCTTTTGTTGTTATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCAC                  >  SRR19973071.1950082/1‑150 (MQ=60)
                                                                                                                                      GCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACG                 >  SRR19973071.3997093/1‑150 (MQ=60)
                                                                                                                                                 TATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACA      >  SRR19973071.1234095/1‑150 (MQ=60)
                                                                                                                                                  ATTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACAT     >  SRR19973071.222054/1‑150 (MQ=60)
                                                                                                                                                   TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    >  SRR19973071.246543/1‑150 (MQ=60)
                                                                                                                                                   TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    >  SRR19973071.246727/1‑150 (MQ=60)
                                                                                                                                                   TTAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATG    >  SRR19973071.4281296/1‑150 (MQ=60)
                                                                                                                                                    TAACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGA   >  SRR19973071.4562632/1‑150 (MQ=60)
                                                                                                                                                     AACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  <  SRR19973071.4786390/150‑1 (MQ=60)
                                                                                                                                                     AACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  SRR19973071.1655171/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AAATACTCGCTCTCACCAGCCTGCAACGCCAGCTGGCGGGGGGCGAAAAGCAGTTCACCATCCACCGGTCGGGTGCGCAATTCACTGCTGCCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTCGGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAA  >  CP009273/300621‑300919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑