Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,631 C→A 100% R348L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,6310CA100.0% 652.9 / NA 196R348L (CGC→CTC) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (103/93);  total (103/93)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

GGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAC  >  CP009273/662486‑662775
                                                                                                                                                 |                                                                                                                                                
ggCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgagc                                                                                                                                                >  2:2121157/1‑148 (MQ=255)
ggCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgagc                                                                                                                                                >  2:1425768/1‑148 (MQ=255)
ggCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgagc                                                                                                                                                <  2:1795934/148‑1 (MQ=255)
 gCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGagcg                                                                                                                                               <  2:2709576/148‑1 (MQ=255)
 gCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGagcg                                                                                                                                               >  2:1935762/1‑148 (MQ=255)
 gCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGagcg                                                                                                                                               >  2:2495413/1‑148 (MQ=255)
 gCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGagcg                                                                                                                                               <  1:1920141/148‑1 (MQ=255)
  ccACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGt                                                                                                                                              <  1:3040099/148‑1 (MQ=255)
    aCCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGa                                                                                                                                            <  2:2575476/148‑1 (MQ=255)
    aCCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGa                                                                                                                                            <  1:387565/148‑1 (MQ=255)
    aCCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGAGTGa                                                                                                                                            >  2:2195894/1‑148 (MQ=255)
     ccTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAt                                                                                                                                           >  1:1532061/1‑148 (MQ=255)
     ccTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAt                                                                                                                                           >  2:2637774/1‑148 (MQ=255)
     ccTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAt                                                                                                                                           <  1:2694753/148‑1 (MQ=255)
      ctcacagaagaAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATc                                                                                                                                          <  1:3383949/143‑1 (MQ=255)
      cTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATc                                                                                                                                          <  1:114624/148‑1 (MQ=255)
       tGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCa                                                                                                                                         >  1:756959/1‑148 (MQ=255)
               gaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcg                                                                                                                                 <  1:2970123/148‑1 (MQ=255)
               gaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcg                                                                                                                                 >  2:192267/1‑148 (MQ=255)
                aagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcgc                                                                                                                                >  1:3204595/1‑148 (MQ=255)
                 agGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCt                                                                                                                               <  1:851312/148‑1 (MQ=255)
                  ggTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTc                                                                                                                              >  2:2855495/1‑148 (MQ=255)
                   gTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCa                                                                                                                             <  1:2643265/148‑1 (MQ=255)
                   gTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCa                                                                                                                             <  1:2669020/148‑1 (MQ=255)
                   gTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCa                                                                                                                             <  1:2669037/148‑1 (MQ=255)
                    tATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGTGTGATCACCCCGGCGCTCaa                                                                                                                            >  2:804921/1‑148 (MQ=255)
                      tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATg                                                                                                                          >  1:2313434/1‑148 (MQ=255)
                      tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATg                                                                                                                          >  2:957456/1‑148 (MQ=255)
                      tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATg                                                                                                                          >  1:3360065/1‑148 (MQ=255)
                       ccGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGc                                                                                                                         <  2:670358/148‑1 (MQ=255)
                           aGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGaa                                                                                                                     <  2:1292413/148‑1 (MQ=255)
                             aTTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAc                                                                                                                   >  2:2531745/1‑148 (MQ=255)
                               tcttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCg                                                                                                                 >  1:2228665/1‑148 (MQ=255)
                                cttcCAGCGATCTTGCGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGc                                                                                                                <  1:3132326/148‑1 (MQ=255)
                                     aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt                                                                                                           <  1:2376079/148‑1 (MQ=255)
                                     aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACAAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt                                                                                                           >  2:172609/1‑148 (MQ=255)
                                      gCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATa                                                                                                          >  1:2774447/1‑148 (MQ=255)
                                       cGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAg                                                                                                         >  1:101099/1‑148 (MQ=255)
                                       cGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTAACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAg                                                                                                         >  2:1167665/1‑148 (MQ=255)
                                          tCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGt                                                                                                      >  2:1642875/1‑148 (MQ=255)
                                          tCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGt                                                                                                      <  2:2527695/148‑1 (MQ=255)
                                                gACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACt                                                                                                <  2:2732514/148‑1 (MQ=255)
                                                 aCATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTg                                                                                               <  2:1818703/148‑1 (MQ=255)
                                                    ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAg                                                                                            >  2:1599555/1‑148 (MQ=255)
                                                    ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAg                                                                                            >  2:978773/1‑148 (MQ=255)
                                                       aGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcg                                                                                         <  2:2355819/148‑1 (MQ=255)
                                                         aCGCCCGTGGCCCCATTTTTTCCAGTCTCGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgcg                                                                                       <  1:1849264/148‑1 (MQ=255)
                                                         aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgcg                                                                                       <  1:1418899/148‑1 (MQ=255)
                                                          cGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCgg                                                                                      >  2:3185452/1‑148 (MQ=255)
                                                             ccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggag                                                                                   <  2:2381481/148‑1 (MQ=255)
                                                                  gCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaa                                                                              <  2:2862815/148‑1 (MQ=255)
                                                                  gCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaa                                                                              >  2:1444750/1‑148 (MQ=255)
                                                                  cacccATTTTTTCCAGTCACGATAACTTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaa                                                                              >  2:1445371/3‑148 (MQ=255)
                                                                   ccccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaaa                                                                             <  2:2282562/148‑1 (MQ=255)
                                                                   ccccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaaa                                                                             <  1:2154851/148‑1 (MQ=255)
                                                                    cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAc                                                                            >  1:815720/1‑148 (MQ=255)
                                                                    cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAc                                                                            >  2:336427/1‑148 (MQ=255)
                                                                    cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAc                                                                            >  2:556070/1‑148 (MQ=255)
                                                                      cATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccc                                                                          >  2:3349652/1‑148 (MQ=255)
                                                                       aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAcccc                                                                         >  2:529279/1‑148 (MQ=255)
                                                                       aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAcccc                                                                         >  1:3059935/1‑148 (MQ=255)
                                                                       aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAcccc                                                                         <  2:756959/148‑1 (MQ=255)
                                                                         tttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCCCATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                       >  2:2131548/1‑148 (MQ=255)
                                                                            ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtg                                                                    <  1:2236860/148‑1 (MQ=255)
                                                                             tCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgt                                                                   <  1:1697126/148‑1 (MQ=255)
                                                                             tCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgt                                                                   >  2:325237/1‑148 (MQ=255)
                                                                              ccAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                  <  1:1191534/148‑1 (MQ=255)
                                                                              ccAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                  >  2:824963/1‑148 (MQ=255)
                                                                                   cACGATAACGTTTGTCCGCACCTGGCAGGTGCCACCCGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGACACCGCCACATCGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGcgcg                                                             <  2:409034/148‑1 (MQ=255)
                                                                                    aCGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCgg                                                            <  2:1057238/148‑1 (MQ=255)
                                                                                     cGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGt                                                           >  1:467056/1‑148 (MQ=255)
                                                                                      gATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGtt                                                          <  2:2447515/148‑1 (MQ=255)
                                                                                      gATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGtt                                                          <  2:652419/148‑1 (MQ=255)
                                                                                         aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGAGCGGTTCAc                                                       >  2:376489/1‑148 (MQ=255)
                                                                                             tttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGc                                                   >  1:24106/1‑148 (MQ=255)
                                                                                              ttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTCTGTGGCGCGGTTCACCAGCg                                                  >  2:2828698/1‑148 (MQ=255)
                                                                                                     aaCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAtt                                           >  1:1692428/1‑148 (MQ=255)
                                                                                                              gTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGtt                                  >  1:2600016/1‑148 (MQ=255)
                                                                                                               ttGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTc                                 >  1:2994934/1‑148 (MQ=255)
                                                                                                                tGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTca                                <  2:101099/148‑1 (MQ=255)
                                                                                                                tGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTca                                <  2:2982082/148‑1 (MQ=255)
                                                                                                                   caccaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaaca                             <  1:1455129/148‑1 (MQ=255)
                                                                                                                     ccaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAg                           >  1:1090114/1‑148 (MQ=255)
                                                                                                                      caGCCTGGGTCAAACAGCGTCTTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAgg                          <  1:172609/148‑1 (MQ=255)
                                                                                                                      caGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAgg                          <  2:2228665/148‑1 (MQ=255)
                                                                                                                        gCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCg                        <  1:2685978/148‑1 (MQ=255)
                                                                                                                          cTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGa                      <  1:1579555/148‑1 (MQ=255)
                                                                                                                                cAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAATGCGGAATAATc                >  1:2848844/1‑148 (MQ=255)
                                                                                                                                cAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATc                >  1:1343425/1‑148 (MQ=255)
                                                                                                                                cAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATc                >  2:663035/1‑148 (MQ=255)
                                                                                                                                   aCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCttt             >  1:1089393/1‑148 (MQ=255)
                                                                                                                                   aCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCttt             <  1:2851464/148‑1 (MQ=255)
                                                                                                                                    cAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg            >  2:2717441/1‑148 (MQ=255)
                                                                                                                                    cAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg            <  1:2121157/148‑1 (MQ=255)
                                                                                                                                     aGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGc           <  2:1170737/148‑1 (MQ=255)
                                                                                                                                        gtcgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTgg        <  1:2531684/148‑1 (MQ=255)
                                                                                                                                          cgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGgag      <  1:951297/148‑1 (MQ=255)
                                                                                                                                           gtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAATGCGGAATAATCTTTGCTGgaga     <  2:3115942/148‑1 (MQ=255)
                                                                                                                                           gtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGgaga     <  1:619599/148‑1 (MQ=255)
                                                                                                                                              ttGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAc  >  1:3154594/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAC  >  CP009273/662486‑662775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  CP009273/662483‑662780
                                                                                                                                                    |                                                                                                                                                     
GTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAG                                                                                                                                                      <  SRR19973066.1180584/150‑1 (MQ=60)
    GCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTG                                                                                                                                                  <  SRR19973066.1933800/150‑1 (MQ=60)
     CCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGA                                                                                                                                                 <  SRR19973066.3060416/150‑1 (MQ=60)
      CACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAT                                                                                                                                                >  SRR19973066.1543015/1‑150 (MQ=60)
      CACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAT                                                                                                                                                >  SRR19973066.1542963/1‑150 (MQ=60)
       ACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATC                                                                                                                                               <  SRR19973066.390634/150‑1 (MQ=60)
        CCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCA                                                                                                                                              <  SRR19973066.2712761/150‑1 (MQ=60)
        CCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCA                                                                                                                                              >  SRR19973066.762567/1‑150 (MQ=60)
         ctcacAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAC                                                                                                                                             <  SRR19973066.3406525/145‑1 (MQ=60)
         CTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAC                                                                                                                                             <  SRR19973066.115794/150‑1 (MQ=60)
           GATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCC                                                                                                                                           >  SRR19973066.2662925/1‑150 (MQ=60)
            ATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCC                                                                                                                                          <  SRR19973066.1677609/150‑1 (MQ=60)
                 AGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGC                                                                                                                                     >  SRR19973066.3225858/1‑150 (MQ=60)
                 AGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGC                                                                                                                                     >  SRR19973066.3225666/1‑150 (MQ=60)
                  GAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCT                                                                                                                                    <  SRR19973066.2989758/150‑1 (MQ=60)
                    AGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCA                                                                                                                                  <  SRR19973066.857468/150‑1 (MQ=60)
                    AGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCA                                                                                                                                  <  SRR19973066.1068607/150‑1 (MQ=60)
                      GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAT                                                                                                                                <  SRR19973066.2686886/150‑1 (MQ=60)
                      GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAT                                                                                                                                <  SRR19973066.2686881/150‑1 (MQ=60)
                      GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAT                                                                                                                                <  SRR19973066.2686869/150‑1 (MQ=60)
                      GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAT                                                                                                                                <  SRR19973066.2686852/150‑1 (MQ=60)
                      GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAT                                                                                                                                <  SRR19973066.2661036/150‑1 (MQ=60)
                       TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                               >  SRR19973066.3382435/1‑150 (MQ=60)
                       TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                               >  SRR19973066.3382468/1‑150 (MQ=60)
                       TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                               >  SRR19973066.3386613/1‑150 (MQ=60)
                       TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                               >  SRR19973066.2329265/1‑150 (MQ=60)
                         TCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCC                                                                                                                             <  SRR19973066.1164196/150‑1 (MQ=60)
                          CCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCG                                                                                                                            <  SRR19973066.2635636/150‑1 (MQ=60)
                                ATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCG                                                                                                                      >  SRR19973066.2244062/1‑150 (MQ=60)
                                 TTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGC                                                                                                                     >  SRR19973066.2710161/1‑150 (MQ=60)
                                   CTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCA                                                                                                                   <  SRR19973066.2394154/150‑1 (MQ=60)
                                   CTTCCAGCGATCTTGCGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCA                                                                                                                   <  SRR19973066.3153235/150‑1 (MQ=60)
                                       CAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATA                                                                                                               >  SRR19973066.2793125/1‑150 (MQ=60)
                                        AGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAG                                                                                                              >  SRR19973066.102176/1‑150 (MQ=60)
                                        AGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAG                                                                                                              <  SRR19973066.2392126/150‑1 (MQ=60)
                                           GATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGT                                                                                                           >  SRR19973066.2740038/1‑150 (MQ=60)
                                           GATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGT                                                                                                           >  SRR19973066.3062865/1‑150 (MQ=60)
                                            ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                          <  SRR19973066.2996957/150‑1 (MQ=60)
                                                        TCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGTGTCAAACAGCGTCGTATTGAGCGTGTTCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTTACTGTAGACG                                                                                              <  SRR19973066.3435384/150‑1 (MQ=60)
                                                            ACGCCCGTGGCCCCATTTTTTCCAGTCTCGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGG                                                                                          <  SRR19973066.1862500/150‑1 (MQ=60)
                                                            ACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGG                                                                                          <  SRR19973066.1429061/150‑1 (MQ=60)
                                                                    GGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAA                                                                                  >  SRR19973066.231424/1‑150 (MQ=60)
                                                                     GCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAC                                                                                 >  SRR19973066.821697/1‑150 (MQ=60)
                                                                      CCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACC                                                                                <  SRR19973066.2169815/150‑1 (MQ=60)
                                                                       CCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCC                                                                               >  SRR19973066.1748759/1‑150 (MQ=60)
                                                                        CCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCC                                                                              >  SRR19973066.3080400/1‑150 (MQ=60)
                                                                               TTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTG                                                                       <  SRR19973066.2252289/150‑1 (MQ=60)
                                                                                TCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGG                                                                      <  SRR19973066.1709460/150‑1 (MQ=60)
                                                                                 CCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGC                                                                     <  SRR19973066.1199877/150‑1 (MQ=60)
                                                                                  CAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCG                                                                    <  SRR19973066.315017/150‑1 (MQ=60)
                                                                                      CACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGT                                                                >  SRR19973066.470609/1‑150 (MQ=60)
                                                                                        CGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTC                                                              <  SRR19973066.964462/150‑1 (MQ=60)
                                                                                          ATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCAC                                                            >  SRR19973066.2343673/1‑150 (MQ=60)
                                                                                            AACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCA                                                          >  SRR19973066.1117163/1‑150 (MQ=60)
                                                                                              CGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGC                                                        >  SRR19973066.24457/1‑150 (MQ=60)
                                                                                              CGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGC                                                        >  SRR19973066.1393195/1‑150 (MQ=60)
                                                                                               GTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCG                                                       >  SRR19973066.1736726/1‑150 (MQ=60)
                                                                                                      CGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATT                                                >  SRR19973066.1704734/1‑150 (MQ=60)
                                                                                                      CGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATT                                                >  SRR19973066.822823/1‑150 (MQ=60)
                                                                                                             GGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCG                                         >  SRR19973066.3164110/1‑150 (MQ=60)
                                                                                                               CAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTT                                       >  SRR19973066.2617613/1‑150 (MQ=60)
                                                                                                               CAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTT                                       >  SRR19973066.819815/1‑150 (MQ=60)
                                                                                                                AGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTC                                      >  SRR19973066.3014792/1‑150 (MQ=60)
                                                                                                                AGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTC                                      >  SRR19973066.3014832/1‑150 (MQ=60)
                                                                                                                      CACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAG                                <  SRR19973066.1465444/150‑1 (MQ=60)
                                                                                                                      CACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAG                                >  SRR19973066.1097904/1‑150 (MQ=60)
                                                                                                                      CACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAG                                >  SRR19973066.1098060/1‑150 (MQ=60)
                                                                                                                      CACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAG                                <  SRR19973066.1465455/150‑1 (MQ=60)
                                                                                                                         CAGCCTGGGTCAAACAGCGTCTTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCG                             <  SRR19973066.174144/150‑1 (MQ=60)
                                                                                                                           GCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGA                           <  SRR19973066.2703892/150‑1 (MQ=60)
                                                                                                                           GCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGA                           <  SRR19973066.935026/150‑1 (MQ=60)
                                                                                                                             CTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAAT                         <  SRR19973066.2233072/150‑1 (MQ=60)
                                                                                                                             CTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAAT                         <  SRR19973066.1590963/150‑1 (MQ=60)
                                                                                                                                GGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAAT                      <  SRR19973066.758356/150‑1 (MQ=60)
                                                                                                                                GGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAAT                      <  SRR19973066.1876275/150‑1 (MQ=60)
                                                                                                                                 GTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAATGCGGAATAATC                     >  SRR19973066.2867987/1‑150 (MQ=60)
                                                                                                                                 GTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATC                     >  SRR19973066.2867478/1‑150 (MQ=60)
                                                                                                                                 GTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATC                     >  SRR19973066.2867990/1‑150 (MQ=60)
                                                                                                                                 GTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATC                     >  SRR19973066.1353172/1‑150 (MQ=60)
                                                                                                                                  TCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCT                    >  SRR19973066.1996799/1‑150 (MQ=60)
                                                                                                                                  TCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCT                    <  SRR19973066.170830/150‑1 (MQ=60)
                                                                                                                                    AAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTT                  >  SRR19973066.1097337/1‑150 (MQ=60)
                                                                                                                                    AAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTT                  >  SRR19973066.428483/1‑150 (MQ=60)
                                                                                                                                     AACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTG                 <  SRR19973066.2538492/150‑1 (MQ=60)
                                                                                                                                     AACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAATCCGAAAACGCCACATACTGTTTAACTGAATACGCTGGATGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTTTATTCCGATCGTTCAACAATCAGGAATAATCTTTT                 >  SRR19973066.269382/1‑150 (MQ=60)
                                                                                                                                      ACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGC                <  SRR19973066.2870626/150‑1 (MQ=60)
                                                                                                                                      ACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGC                <  SRR19973066.1206017/150‑1 (MQ=60)
                                                                                                                                       CAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCT               <  SRR19973066.2135827/150‑1 (MQ=60)
                                                                                                                                       CAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCT               <  SRR19973066.603899/150‑1 (MQ=60)
                                                                                                                                       CAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCT               <  SRR19973066.1435956/150‑1 (MQ=60)
                                                                                                                                          CGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGA            >  SRR19973066.214510/1‑150 (MQ=60)
                                                                                                                                           GTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAG           <  SRR19973066.2548958/150‑1 (MQ=60)
                                                                                                                                           GTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAG           <  SRR19973066.2548897/150‑1 (MQ=60)
                                                                                                                                             CGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAT         >  SRR19973066.2244969/1‑150 (MQ=60)
                                                                                                                                             CGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAT         <  SRR19973066.958251/150‑1 (MQ=60)
                                                                                                                                              GTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATA        <  SRR19973066.623984/150‑1 (MQ=60)
                                                                                                                                               TATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAC       >  SRR19973066.2967434/1‑150 (MQ=60)
                                                                                                                                               TATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAC       >  SRR19973066.3175626/1‑150 (MQ=60)
                                                                                                                                                  TGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGACTAATCTTTGCTGGAGATACCGT    >  SRR19973066.2539011/1‑150 (MQ=60)
                                                                                                                                                   GAGCGTGATCACCCCGGCGCTCAATTCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAACGTC   >  SRR19973066.672282/1‑150 (MQ=60)
                                                                                                                                                   GAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTC   >  SRR19973066.673197/1‑150 (MQ=60)
                                                                                                                                                    AGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  SRR19973066.1519184/1‑150 (MQ=60)
                                                                                                                                                    AGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  SRR19973066.1378143/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  CP009273/662483‑662780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑