Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 662,631 C→A R348L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,6310CA99.5% 635.2 / NA 190R348L (CGC→CTC) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (86/103);  minor base T (0/1);  total (86/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

AGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCG  >  CP009273/662485‑662777
                                                                                                                                                  |                                                                                                                                                  
aGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGagcg                                                                                                                                                 >  2:229196/1‑150 (MQ=255)
 ggCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGt                                                                                                                                                <  1:589423/150‑1 (MQ=255)
 ggCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGt                                                                                                                                                >  2:3272919/1‑150 (MQ=255)
    cACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAt                                                                                                                                             >  1:3702715/1‑150 (MQ=255)
         gATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAccc                                                                                                                                        <  1:1011662/150‑1 (MQ=255)
           tataagaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCg                                                                                                                                      >  1:2380606/4‑150 (MQ=255)
           tAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCg                                                                                                                                      <  2:1413503/150‑1 (MQ=255)
             gaagaaGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgc                                                                                                                                    <  2:1267189/150‑1 (MQ=255)
               agaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcgc                                                                                                                                  >  1:364997/1‑150 (MQ=255)
                  agGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCa                                                                                                                               >  1:1866682/1‑150 (MQ=255)
                    gTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAt                                                                                                                             <  1:819082/150‑1 (MQ=255)
                     tATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATg                                                                                                                            <  1:3369651/150‑1 (MQ=255)
                       tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGcc                                                                                                                          <  2:827583/150‑1 (MQ=255)
                           cAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGaaa                                                                                                                      <  1:735945/150‑1 (MQ=255)
                           cAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGaaa                                                                                                                      <  1:735956/150‑1 (MQ=255)
                            aGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAc                                                                                                                     >  2:136279/1‑150 (MQ=255)
                             gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTTATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                    >  1:2947931/1‑150 (MQ=255)
                             gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                    <  2:639554/150‑1 (MQ=255)
                             gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                    <  2:1808480/150‑1 (MQ=255)
                             gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                    <  1:53974/150‑1 (MQ=255)
                                tcttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGcc                                                                                                                 <  2:791012/150‑1 (MQ=255)
                                   tcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcaca                                                                                                              >  1:2452345/1‑150 (MQ=255)
                                    ccAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt                                                                                                             >  2:1864921/1‑150 (MQ=255)
                                     cAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATa                                                                                                            >  2:1647145/1‑150 (MQ=255)
                                      aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAg                                                                                                           >  1:291919/1‑150 (MQ=255)
                                         gATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGt                                                                                                        >  2:3421004/1‑150 (MQ=255)
                                          aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                       >  1:931711/1‑150 (MQ=255)
                                          aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                       >  1:1190196/1‑150 (MQ=255)
                                          aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                       <  1:1556513/150‑1 (MQ=255)
                                              tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAc                                                                                                   <  1:2669731/150‑1 (MQ=255)
                                              tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAc                                                                                                   <  1:1645503/150‑1 (MQ=255)
                                               gtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACt                                                                                                  <  1:2804141/150‑1 (MQ=255)
                                                       cAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgc                                                                                          <  1:1286356/150‑1 (MQ=255)
                                                       cAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgc                                                                                          >  1:1647357/1‑150 (MQ=255)
                                                         gACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCgg                                                                                        >  1:2481030/1‑150 (MQ=255)
                                                          aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCggg                                                                                       <  1:2728590/150‑1 (MQ=255)
                                                          aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCggg                                                                                       <  2:1939706/150‑1 (MQ=255)
                                                             cccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggagg                                                                                    >  1:1554112/1‑150 (MQ=255)
                                                             cccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggagg                                                                                    >  1:615274/1‑150 (MQ=255)
                                                             cccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggagg                                                                                    >  1:2063322/1‑150 (MQ=255)
                                                             cccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggagg                                                                                    >  2:69525/1‑150 (MQ=255)
                                                               cGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAt                                                                                  <  1:2860446/150‑1 (MQ=255)
                                                               cGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAt                                                                                  <  1:1419975/150‑1 (MQ=255)
                                                                gTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATa                                                                                 <  2:2752993/150‑1 (MQ=255)
                                                                gTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATa                                                                                 <  1:435042/150‑1 (MQ=255)
                                                                 tGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaa                                                                                <  2:1125599/150‑1 (MQ=255)
                                                                   gCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAc                                                                              <  1:3255015/150‑1 (MQ=255)
                                                                   gCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAc                                                                              >  2:1351509/1‑150 (MQ=255)
                                                                    ccccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGACACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAcc                                                                             >  2:3211560/1‑150 (MQ=255)
                                                                       cATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccccc                                                                          >  1:1575301/1‑150 (MQ=255)
                                                                       cATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccccc                                                                          <  2:3525646/150‑1 (MQ=255)
                                                                              tCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTgg                                                                   >  1:1097954/1‑150 (MQ=255)
                                                                              tCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTgg                                                                   >  1:2169405/1‑150 (MQ=255)
                                                                                  gTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGcgcg                                                               >  1:159201/1‑150 (MQ=255)
                                                                                    cACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGt                                                             <  2:2905968/150‑1 (MQ=255)
                                                                                         tAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCAcc                                                        >  2:2967893/1‑150 (MQ=255)
                                                                                          aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCa                                                       <  1:1864921/150‑1 (MQ=255)
                                                                                            cGTTTTTCCGAACCTGGCAGGTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGc                                                     <  1:3584852/150‑1 (MQ=255)
                                                                                             gTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCg                                                    >  1:950612/1‑150 (MQ=255)
                                                                                             gTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCg                                                    <  1:1537536/150‑1 (MQ=255)
                                                                                                tttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGgtg                                                 <  2:1927238/150‑1 (MQ=255)
                                                                                                tttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGgtg                                                 >  1:394097/1‑150 (MQ=255)
                                                                                                  tCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTa                                               <  2:1060690/150‑1 (MQ=255)
                                                                                                    cGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAtt                                             >  2:1450088/1‑150 (MQ=255)
                                                                                                     gAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTc                                            <  1:1078166/150‑1 (MQ=255)
                                                                                                       aCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCgg                                          <  2:2941319/150‑1 (MQ=255)
                                                                                                        ccTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGa                                         <  1:1599886/150‑1 (MQ=255)
                                                                                                        ccTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGa                                         >  1:3170035/1‑150 (MQ=255)
                                                                                                        ccTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGa                                         >  1:373878/1‑150 (MQ=255)
                                                                                                         cTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGAt                                        <  2:1542466/150‑1 (MQ=255)
                                                                                                         cTGGCAGGTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGAt                                        <  2:2984334/150‑1 (MQ=255)
                                                                                                             cAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGtt                                    >  1:775969/1‑150 (MQ=255)
                                                                                                               gTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTca                                  <  2:3023302/150‑1 (MQ=255)
                                                                                                               gTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTca                                  >  1:3742494/1‑150 (MQ=255)
                                                                                                                ttGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaa                                 <  1:876100/150‑1 (MQ=255)
                                                                                                                  gCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaaca                               >  2:675925/1‑150 (MQ=255)
                                                                                                                    caccaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAg                             <  2:3263759/150‑1 (MQ=255)
                                                                                                                       caGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCg                          <  2:473614/150‑1 (MQ=255)
                                                                                                                        aGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCgg                         <  1:1783466/150‑1 (MQ=255)
                                                                                                                        aGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCgg                         >  1:445810/1‑150 (MQ=255)
                                                                                                                        aGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCgg                         <  1:2789531/150‑1 (MQ=255)
                                                                                                                         gCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACTTAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGa                        <  1:526130/150‑1 (MQ=255)
                                                                                                                         gCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGa                        <  1:229196/150‑1 (MQ=255)
                                                                                                                          ccTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaa                       >  1:2148058/1‑150 (MQ=255)
                                                                                                                             gggTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataa                    <  1:69525/150‑1 (MQ=255)
                                                                                                                              ggTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataat                   <  2:200576/150‑1 (MQ=255)
                                                                                                                              ggTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataat                   >  2:1324117/1‑150 (MQ=255)
                                                                                                                               gTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATc                  <  2:3065142/150‑1 (MQ=255)
                                                                                                                                tCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCt                 <  1:3264275/150‑1 (MQ=255)
                                                                                                                                tCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCt                 <  1:1364508/150‑1 (MQ=255)
                                                                                                                                 cAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCtt                <  1:2383226/150‑1 (MQ=255)
                                                                                                                                 cAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCtt                <  2:2727208/150‑1 (MQ=255)
                                                                                                                                   aaCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg              <  2:2066123/150‑1 (MQ=255)
                                                                                                                                    aCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGc             >  1:56059/1‑150 (MQ=255)
                                                                                                                                      aGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTg           >  1:3517391/1‑150 (MQ=255)
                                                                                                                                      aGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTg           >  2:945058/1‑150 (MQ=255)
                                                                                                                                           cgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAt      <  2:3662823/150‑1 (MQ=255)
                                                                                                                                           cgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAt      <  1:188674/150‑1 (MQ=255)
                                                                                                                                             tATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAc    <  1:294628/150‑1 (MQ=255)
                                                                                                                                              aTTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAcc   <  1:3274649/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCG  >  CP009273/662485‑662777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGT  >  CP009273/662486‑662778
                                                                                                                                                 |                                                                                                                                                   
GGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGT                                                                                                                                                 <  SRR19973061.590958/150‑1 (MQ=60)
 GCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTG                                                                                                                                                >  SRR19973061.3534676/1‑150 (MQ=60)
   CACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAT                                                                                                                                              >  SRR19973061.3713831/1‑150 (MQ=60)
   CACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAT                                                                                                                                              >  SRR19973061.3712179/1‑150 (MQ=60)
        GATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCC                                                                                                                                         <  SRR19973061.1014240/150‑1 (MQ=60)
          TATAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCG                                                                                                                                       >  SRR19973061.2386669/1‑150 (MQ=60)
          TAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCG                                                                                                                                       >  SRR19973061.2386679/1‑150 (MQ=60)
              AGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGC                                                                                                                                   >  SRR19973061.365985/1‑150 (MQ=60)
                 AGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCA                                                                                                                                >  SRR19973061.1871290/1‑150 (MQ=60)
                 AGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCA                                                                                                                                >  SRR19973061.1871337/1‑150 (MQ=60)
                   GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAT                                                                                                                              <  SRR19973061.821222/150‑1 (MQ=60)
                    TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                             <  SRR19973061.3378314/150‑1 (MQ=60)
                    TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                             >  SRR19973061.793088/1‑150 (MQ=60)
                     ATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGC                                                                                                                            >  SRR19973061.575475/1‑150 (MQ=60)
                         GCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAA                                                                                                                        >  SRR19973061.743859/1‑150 (MQ=60)
                          CAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAA                                                                                                                       <  SRR19973061.737887/150‑1 (MQ=60)
                          CAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAA                                                                                                                       <  SRR19973061.737876/150‑1 (MQ=60)
                           AGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAC                                                                                                                      >  SRR19973061.46765/1‑150 (MQ=60)
                           AGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAC                                                                                                                      >  SRR19973061.3220547/1‑150 (MQ=60)
                            GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTTATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                     >  SRR19973061.2955524/1‑150 (MQ=60)
                            GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                     <  SRR19973061.54181/150‑1 (MQ=60)
                            GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                     <  SRR19973061.54167/150‑1 (MQ=60)
                            GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                     <  SRR19973061.55456/150‑1 (MQ=60)
                              TTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGC                                                                                                                   >  SRR19973061.694337/1‑150 (MQ=60)
                                CTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCA                                                                                                                 <  SRR19973061.382940/150‑1 (MQ=60)
                                 TTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCAC                                                                                                                >  SRR19973061.403616/1‑150 (MQ=60)
                                 TTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCAC                                                                                                                >  SRR19973061.443773/1‑150 (MQ=60)
                                  TCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACA                                                                                                               >  SRR19973061.2459424/1‑150 (MQ=60)
                                  TCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACA                                                                                                               >  SRR19973061.2458594/1‑150 (MQ=60)
                                   CCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAT                                                                                                              >  SRR19973061.201173/1‑150 (MQ=60)
                                     AGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAG                                                                                                            >  SRR19973061.292777/1‑150 (MQ=60)
                                     AGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAG                                                                                                            >  SRR19973061.292700/1‑150 (MQ=60)
                                         ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                        >  SRR19973061.934088/1‑150 (MQ=60)
                                         ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                        <  SRR19973061.1560761/150‑1 (MQ=60)
                                         ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                        <  SRR19973061.1560379/150‑1 (MQ=60)
                                         ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                        >  SRR19973061.1193194/1‑150 (MQ=60)
                                             TGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAC                                                                                                    <  SRR19973061.2676607/150‑1 (MQ=60)
                                             TGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAC                                                                                                    <  SRR19973061.2676601/150‑1 (MQ=60)
                                             TGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAC                                                                                                    <  SRR19973061.1649617/150‑1 (MQ=60)
                                             TGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAC                                                                                                    <  SRR19973061.1649636/150‑1 (MQ=60)
                                              GTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACT                                                                                                   <  SRR19973061.2811179/150‑1 (MQ=60)
                                              GTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACT                                                                                                   <  SRR19973061.2811345/150‑1 (MQ=60)
                                                  CATTCAGACGCCCGTGGCCCTATTTTTTCCAGTCTCGATAACGTTTTTCAGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGATATTGATCACCACGGCGCTCAATGCCGATACCGCCACATAGGGTTTAACTGTAG                                                                                               <  SRR19973061.3652882/150‑1 (MQ=60)
                                                      CAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGC                                                                                           <  SRR19973061.1289545/150‑1 (MQ=60)
                                                      CAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGC                                                                                           >  SRR19973061.1651495/1‑150 (MQ=60)
                                                        GACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGG                                                                                         >  SRR19973061.2487345/1‑150 (MQ=60)
                                                         ACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGG                                                                                        <  SRR19973061.2736622/150‑1 (MQ=60)
                                                         ACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGG                                                                                        <  SRR19973061.2735605/150‑1 (MQ=60)
                                                            CCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGG                                                                                     >  SRR19973061.2068516/1‑150 (MQ=60)
                                                            CCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGG                                                                                     >  SRR19973061.1557973/1‑150 (MQ=60)
                                                            CCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGG                                                                                     >  SRR19973061.616853/1‑150 (MQ=60)
                                                            CCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGG                                                                                     >  SRR19973061.616855/1‑150 (MQ=60)
                                                            CCCGTGGCCCCATTTTTCCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGG                                                                                     >  SRR19973061.1558416/1‑150 (MQ=60)
                                                              CGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAT                                                                                   <  SRR19973061.2867771/150‑1 (MQ=60)
                                                              CGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAT                                                                                   <  SRR19973061.1423555/150‑1 (MQ=60)
                                                               GTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATA                                                                                  >  SRR19973061.845977/1‑150 (MQ=60)
                                                               GTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATA                                                                                  <  SRR19973061.436230/150‑1 (MQ=60)
                                                                  GCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAC                                                                               >  SRR19973061.2068996/1‑150 (MQ=60)
                                                                  GCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAC                                                                               <  SRR19973061.3263388/150‑1 (MQ=60)
                                                                      CATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCC                                                                           >  SRR19973061.1579215/1‑150 (MQ=60)
                                                                             TCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGG                                                                    >  SRR19973061.2174851/1‑150 (MQ=60)
                                                                             TCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGG                                                                    >  SRR19973061.1100766/1‑150 (MQ=60)
                                                                             TCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGG                                                                    <  SRR19973061.72157/150‑1 (MQ=60)
                                                                                 GTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCG                                                                >  SRR19973061.159699/1‑150 (MQ=60)
                                                                                  TCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGG                                                               >  SRR19973061.3272160/1‑150 (MQ=60)
                                                                                       ATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCAC                                                          >  SRR19973061.1441192/1‑150 (MQ=60)
                                                                                         AACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCA                                                        >  SRR19973061.915770/1‑150 (MQ=60)
                                                                                         AACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCA                                                        >  SRR19973061.2823777/1‑150 (MQ=60)
                                                                                         AACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCA                                                        >  SRR19973061.980908/1‑150 (MQ=60)
                                                                                         AACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCA                                                        <  SRR19973061.1869572/150‑1 (MQ=60)
                                                                                           CGTTTTTCCGAACCTGGCAGGTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGC                                                      <  SRR19973061.3594044/150‑1 (MQ=60)
                                                                                            GTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCG                                                     >  SRR19973061.953010/1‑150 (MQ=60)
                                                                                            GTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCG                                                     >  SRR19973061.952395/1‑150 (MQ=60)
                                                                                            GTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCG                                                     <  SRR19973061.1541366/150‑1 (MQ=60)
                                                                                               TTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTG                                                  >  SRR19973061.395169/1‑150 (MQ=60)
                                                                                                TTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGT                                                 <  SRR19973061.2662978/150‑1 (MQ=60)
                                                                                                 TCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACNGTAGACGCNGGAGGATAANCNCCNNGANTGNCNCGGTTNACCNGNGGTGTA                                                >  SRR19973061.615360/1‑150 (MQ=60)
                                                                                                   CGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATT                                              >  SRR19973061.1282661/1‑150 (MQ=60)
                                                                                                    GAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTC                                             <  SRR19973061.1080932/150‑1 (MQ=60)
                                                                                                    GAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTC                                             <  SRR19973061.2209508/150‑1 (MQ=60)
                                                                                                    GAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTC                                             >  SRR19973061.2425104/1‑150 (MQ=60)
                                                                                                       CCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGA                                          <  SRR19973061.1603861/150‑1 (MQ=60)
                                                                                                       CCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGA                                          >  SRR19973061.374893/1‑150 (MQ=60)
                                                                                                       CCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGA                                          >  SRR19973061.3178165/1‑150 (MQ=60)
                                                                                                            CAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTT                                     >  SRR19973061.778009/1‑150 (MQ=60)
                                                                                                            CAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTT                                     <  SRR19973061.1651283/150‑1 (MQ=60)
                                                                                                            CAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTT                                     >  SRR19973061.2880441/1‑150 (MQ=60)
                                                                                                              GTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCA                                   >  SRR19973061.3479672/1‑150 (MQ=60)
                                                                                                              GTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCA                                   >  SRR19973061.3752073/1‑150 (MQ=60)
                                                                                                               TTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAA                                  <  SRR19973061.878372/150‑1 (MQ=60)
                                                                                                                   CACCAGCCTGGGTCAAACAGCGTCGTATTGAGCATGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAG                              <  SRR19973061.335052/150‑1 (MQ=60)
                                                                                                                       AGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGG                          <  SRR19973061.2796701/150‑1 (MQ=60)
                                                                                                                       AGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGG                          >  SRR19973061.1586204/1‑150 (MQ=60)
                                                                                                                       AGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGG                          <  SRR19973061.1787936/150‑1 (MQ=60)
                                                                                                                       AGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGG                          >  SRR19973061.447020/1‑150 (MQ=60)
                                                                                                                        GCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACTTAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGA                         <  SRR19973061.527521/150‑1 (MQ=60)
                                                                                                                        GCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGA                         <  SRR19973061.229867/150‑1 (MQ=60)
                                                                                                                         CCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAA                        >  SRR19973061.2153455/1‑150 (MQ=60)
                                                                                                                         CCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAA                        >  SRR19973061.2153474/1‑150 (MQ=60)
                                                                                                                          CTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAAT                       <  SRR19973061.1154033/150‑1 (MQ=60)
                                                                                                                           TGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATA                      <  SRR19973061.3057224/150‑1 (MQ=60)
                                                                                                                           TGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATA                      >  SRR19973061.1705019/1‑150 (MQ=60)
                                                                                                                            GGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAA                     <  SRR19973061.69783/150‑1 (MQ=60)
                                                                                                                             GGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAAT                    <  SRR19973061.3681800/150‑1 (MQ=60)
                                                                                                                              GTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATC                   <  SRR19973061.1556524/150‑1 (MQ=60)
                                                                                                                               TCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCT                  <  SRR19973061.1367951/150‑1 (MQ=60)
                                                                                                                               TCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCT                  <  SRR19973061.1367949/150‑1 (MQ=60)
                                                                                                                               TCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCT                  <  SRR19973061.1089247/150‑1 (MQ=60)
                                                                                                                               TCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCT                  <  SRR19973061.3272678/150‑1 (MQ=60)
                                                                                                                                CAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTT                 <  SRR19973061.2389298/150‑1 (MQ=60)
                                                                                                                                  AACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTG               <  SRR19973061.1858057/150‑1 (MQ=60)
                                                                                                                                  AACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTG               <  SRR19973061.1887530/150‑1 (MQ=60)
                                                                                                                                   ACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGC              >  SRR19973061.56280/1‑150 (MQ=60)
                                                                                                                                     AGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTG            >  SRR19973061.3526401/1‑150 (MQ=60)
                                                                                                                                          CGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAT       <  SRR19973061.189246/150‑1 (MQ=60)
                                                                                                                                            TATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAC     <  SRR19973061.295416/150‑1 (MQ=60)
                                                                                                                                             ATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC    <  SRR19973061.3283084/150‑1 (MQ=60)
                                                                                                                                             ATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC    <  SRR19973061.3281349/150‑1 (MQ=60)
                                                                                                                                              TTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCG   <  SRR19973061.136715/150‑1 (MQ=60)
                                                                                                                                               TGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGT  >  SRR19973061.2404554/1‑150 (MQ=60)
                                                                                                                                               TGAGCGTGATCACACCGGCGATCAATGACGAACCCGCCACATAGGGTTTAACTGTATACGCGGTAGGATAAACCCCCTGTTTGGCGCGGTTAACCCGCGGTGTATTCGGAACGTTCAACAAGGCGGACTAAACATTGCTGTAGATACCGT  >  SRR19973061.2404550/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
GGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGT  >  CP009273/662486‑662778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑