Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,631 C→A 92.9% R348L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,6310CA92.9% 743.9 / 37.7 226R348L (CGC→CTC) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base C (9/7);  new base A (88/122);  total (97/129)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

TAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC  >  CP009273/662484‑662776
                                                                                                                                                   |                                                                                                                                                 
  ggCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGt                                                                                                                                               >  1:269535/1‑150 (MQ=255)
    ccACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGa                                                                                                                                             >  1:590930/1‑150 (MQ=255)
     cACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAt                                                                                                                                            >  2:4943198/1‑150 (MQ=255)
     cACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAt                                                                                                                                            <  2:680339/150‑1 (MQ=255)
      aCCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATc                                                                                                                                           <  1:2752039/150‑1 (MQ=255)
        cTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAc                                                                                                                                         <  1:5013077/150‑1 (MQ=255)
        cTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAc                                                                                                                                         >  2:263914/1‑150 (MQ=255)
            tAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCg                                                                                                                                     <  2:623454/150‑1 (MQ=255)
               aagaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGgcg                                                                                                                                  >  1:3845394/1‑150 (MQ=255)
                    ggTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCaa                                                                                                                             >  1:1770410/1‑150 (MQ=255)
                     gTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAAt                                                                                                                            >  1:2570744/1‑150 (MQ=255)
                     gTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAt                                                                                                                            >  2:2483290/1‑150 (MQ=255)
                        tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGcc                                                                                                                         <  2:372597/150‑1 (MQ=255)
                        tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGcc                                                                                                                         >  1:763690/1‑150 (MQ=255)
                        tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGcc                                                                                                                         <  2:2983422/150‑1 (MQ=255)
                              gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                   <  1:4444944/150‑1 (MQ=255)
                              gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                   <  2:1537464/150‑1 (MQ=255)
                                ttcttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGc                                                                                                                 >  1:826050/1‑150 (MQ=255)
                                ttcttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGc                                                                                                                 >  1:3526815/1‑150 (MQ=255)
                                   ttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACTATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcac                                                                                                              <  2:3800144/150‑1 (MQ=255)
                                   ttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCcac                                                                                                              <  1:3278538/150‑1 (MQ=255)
                                       aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAg                                                                                                          >  2:1949155/1‑150 (MQ=255)
                                       aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAg                                                                                                          <  2:5121571/150‑1 (MQ=255)
                                       aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAg                                                                                                          >  2:4106569/1‑150 (MQ=255)
                                         cGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAggg                                                                                                        >  1:2228204/1‑150 (MQ=255)
                                           aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                      <  2:1680043/150‑1 (MQ=255)
                                           aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                      <  1:1169966/150‑1 (MQ=255)
                                           aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                      <  1:3184148/150‑1 (MQ=255)
                                           aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                      <  1:1169758/150‑1 (MQ=255)
                                             cTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTa                                                                                                    <  1:4070449/150‑1 (MQ=255)
                                             cTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTa                                                                                                    >  1:676928/1‑150 (MQ=255)
                                              ttGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAGCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTaa                                                                                                   >  1:2085302/1‑150 (MQ=255)
                                               tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAc                                                                                                  >  2:397618/1‑150 (MQ=255)
                                               tgtgACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAc                                                                                                  >  1:1818385/1‑150 (MQ=255)
                                                      ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAc                                                                                           <  1:3803377/150‑1 (MQ=255)
                                                      ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAc                                                                                           <  2:1655208/150‑1 (MQ=255)
                                                       tCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTGTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcg                                                                                          <  2:1084268/150‑1 (MQ=255)
                                                        cAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgc                                                                                         <  1:2386109/150‑1 (MQ=255)
                                                         aGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgcg                                                                                        >  2:5023487/1‑150 (MQ=255)
                                                         aGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgcg                                                                                        <  1:4836584/150‑1 (MQ=255)
                                                         aGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTGTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgcg                                                                                        <  2:4858872/150‑1 (MQ=255)
                                                           aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCggg                                                                                      >  1:2114458/1‑150 (MQ=255)
                                                               ccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggagga                                                                                  >  1:427521/1‑150 (MQ=255)
                                                                cGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAt                                                                                 <  2:3497605/150‑1 (MQ=255)
                                                                cGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAt                                                                                 >  2:1854008/1‑150 (MQ=255)
                                                                cGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAt                                                                                 <  1:2470132/150‑1 (MQ=255)
                                                                        cATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAATCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccccc                                                                         >  2:4344406/1‑150 (MQ=255)
                                                                         aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACCGCGTCGTATTGAGCGTGATCACCCCGGCGCGAAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                        >  1:2036558/1‑150 (MQ=255)
                                                                         aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                        >  1:125671/1‑150 (MQ=255)
                                                                         aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                        <  2:2527502/150‑1 (MQ=255)
                                                                         aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                        <  2:853724/150‑1 (MQ=255)
                                                                            ttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtg                                                                     <  2:664471/150‑1 (MQ=255)
                                                                            ttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtg                                                                     >  2:5097293/1‑150 (MQ=255)
                                                                             tttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgt                                                                    <  2:658250/150‑1 (MQ=255)
                                                                              ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                   <  1:394114/150‑1 (MQ=255)
                                                                              ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                   <  1:2828809/150‑1 (MQ=255)
                                                                              ttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                   <  2:1770410/150‑1 (MQ=255)
                                                                                 cAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcg                                                                >  2:1882159/1‑150 (MQ=255)
                                                                                 cAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcg                                                                <  2:3331391/150‑1 (MQ=255)
                                                                                  aGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcgc                                                               >  2:3675362/1‑150 (MQ=255)
                                                                                   gTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGcgcg                                                              >  2:3062670/1‑150 (MQ=255)
                                                                                   gTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGcgcg                                                              <  1:2347636/150‑1 (MQ=255)
                                                                                   gTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGcgcg                                                              >  2:4485365/1‑150 (MQ=255)
                                                                                   gTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGcgcg                                                              >  1:4905718/1‑150 (MQ=255)
                                                                                      aCGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGtt                                                           <  2:4036124/150‑1 (MQ=255)
                                                                                         aTAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCAc                                                        >  2:808139/1‑150 (MQ=255)
                                                                                           aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCa                                                      <  2:816421/150‑1 (MQ=255)
                                                                                           aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCa                                                      >  2:3783839/1‑150 (MQ=255)
                                                                                               tttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCgg                                                  >  2:3907994/1‑150 (MQ=255)
                                                                                                    ccGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAt                                             <  2:269535/150‑1 (MQ=255)
                                                                                                      gAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTc                                           >  2:2221427/1‑150 (MQ=255)
                                                                                                         ccTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGa                                        >  2:2553296/1‑150 (MQ=255)
                                                                                                          cTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGAt                                       >  2:1452458/1‑150 (MQ=255)
                                                                                                           tGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATc                                      <  1:3976700/150‑1 (MQ=255)
                                                                                                           tGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATc                                      <  1:46480/150‑1 (MQ=255)
                                                                                                            ggCAGGTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCg                                     <  1:108385/150‑1 (MQ=255)
                                                                                                               aGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTGTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTc                                  >  2:4813483/1‑150 (MQ=255)
                                                                                                                gTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTca                                 <  2:2287282/150‑1 (MQ=255)
                                                                                                                  tGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaac                               <  2:2000985/150‑1 (MQ=255)
                                                                                                                    ccaccaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaacaa                             >  1:1628503/1‑150 (MQ=255)
                                                                                                                    ccaccaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaacaa                             <  1:2608184/150‑1 (MQ=255)
                                                                                                                    ccaccaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaacaa                             >  1:4222813/1‑150 (MQ=255)
                                                                                                                        caGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCg                         <  2:3135037/150‑1 (MQ=255)
                                                                                                                         aGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCgg                        <  2:1370718/150‑1 (MQ=255)
                                                                                                                           ccTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaa                      <  1:4497132/150‑1 (MQ=255)
                                                                                                                            cTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaat                     <  1:1043467/150‑1 (MQ=255)
                                                                                                                                   aaaCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCttt              <  1:263914/150‑1 (MQ=255)
                                                                                                                                    aaCAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg             <  1:1206111/150‑1 (MQ=255)
                                                                                                                                    aaCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg             <  2:3467641/150‑1 (MQ=255)
                                                                                                                                      cAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCt           <  1:4508221/150‑1 (MQ=255)
                                                                                                                                       aGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCTCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTg          <  1:4802804/150‑1 (MQ=255)
                                                                                                                                       aGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTg          <  2:2214467/150‑1 (MQ=255)
                                                                                                                                         cgtcgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGga        >  1:4578274/1‑150 (MQ=255)
                                                                                                                                            cgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAt     <  1:4332525/150‑1 (MQ=255)
                                                                                                                                            cgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAt     <  2:4451447/150‑1 (MQ=255)
                                                                                                                                            cgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAt     <  1:2999266/150‑1 (MQ=255)
                                                                                                                                             gtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATa    >  1:3133930/1‑150 (MQ=255)
                                                                                                                                              tATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAc   >  2:4644262/1‑150 (MQ=255)
                                                                                                                                               aTTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAcc  >  2:485245/1‑150 (MQ=255)
                                                                                                                                               aTTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAcc  >  1:4533357/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC  >  CP009273/662484‑662776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  CP009273/662482‑662780
                                                                                                                                                     |                                                                                                                                                     
CGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGA                                                                                                                                                       >  SRR19973083.5063872/1‑150 (MQ=60)
    GGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGT                                                                                                                                                   >  SRR19973083.270958/1‑150 (MQ=60)
      CCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGA                                                                                                                                                 >  SRR19973083.593903/1‑150 (MQ=60)
       CACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGAT                                                                                                                                                >  SRR19973083.2987422/1‑150 (MQ=60)
        ACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATC                                                                                                                                               <  SRR19973083.2765740/150‑1 (MQ=60)
         CCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCA                                                                                                                                              >  SRR19973083.2638092/1‑150 (MQ=60)
          CTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAC                                                                                                                                             <  SRR19973083.5034580/150‑1 (MQ=60)
             ATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCC                                                                                                                                          >  SRR19973083.4539676/1‑150 (MQ=60)
             ATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTATCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGATCGTGATCACCCC                                                                                                                                          >  SRR19973083.1767522/1‑150 (MQ=60)
               AGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGG                                                                                                                                        >  SRR19973083.2677068/1‑150 (MQ=60)
               AGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGG                                                                                                                                        >  SRR19973083.2677257/1‑150 (MQ=60)
                 AAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCG                                                                                                                                      >  SRR19973083.3862630/1‑150 (MQ=60)
                  AGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGC                                                                                                                                     >  SRR19973083.857742/1‑150 (MQ=60)
                    AAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTC                                                                                                                                   >  SRR19973083.1108352/1‑150 (MQ=60)
                      GGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAA                                                                                                                                 >  SRR19973083.1779189/1‑150 (MQ=60)
                       GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAAT                                                                                                                                >  SRR19973083.2583717/1‑150 (MQ=60)
                       GTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAAT                                                                                                                                <  SRR19973083.2769557/150‑1 (MQ=60)
                        TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                               <  SRR19973083.4890738/150‑1 (MQ=60)
                        TATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATG                                                                                                                               <  SRR19973083.1891240/150‑1 (MQ=60)
                          TCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCC                                                                                                                             >  SRR19973083.767424/1‑150 (MQ=60)
                               AGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAC                                                                                                                        <  SRR19973083.2145671/150‑1 (MQ=60)
                                GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                       >  SRR19973083.2081556/1‑150 (MQ=60)
                                GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                       >  SRR19973083.3921548/1‑150 (MQ=60)
                                GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                       <  SRR19973083.4472809/150‑1 (MQ=60)
                                GATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACC                                                                                                                       <  SRR19973083.4464272/150‑1 (MQ=60)
                                 ATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCG                                                                                                                      <  SRR19973083.2254663/150‑1 (MQ=60)
                                  TTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGC                                                                                                                     >  SRR19973083.3542998/1‑150 (MQ=60)
                                  TTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGC                                                                                                                     >  SRR19973083.829975/1‑150 (MQ=60)
                                     TTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCAC                                                                                                                  <  SRR19973083.4695291/150‑1 (MQ=60)
                                     TTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCAC                                                                                                                  <  SRR19973083.3294193/150‑1 (MQ=60)
                                     TTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCAC                                                                                                                  <  SRR19973083.3293981/150‑1 (MQ=60)
                                        CAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATA                                                                                                               <  SRR19973083.1790431/150‑1 (MQ=60)
                                        CAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTACAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATA                                                                                                               <  SRR19973083.1790727/150‑1 (MQ=60)
                                           CGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGG                                                                                                            >  SRR19973083.2239488/1‑150 (MQ=60)
                                            GATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGT                                                                                                           <  SRR19973083.2458796/150‑1 (MQ=60)
                                             ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                          <  SRR19973083.3199309/150‑1 (MQ=60)
                                             ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                          <  SRR19973083.1175575/150‑1 (MQ=60)
                                             ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                          <  SRR19973083.1175705/150‑1 (MQ=60)
                                             ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTT                                                                                                          <  SRR19973083.1175785/150‑1 (MQ=60)
                                               CTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTA                                                                                                        >  SRR19973083.680314/1‑150 (MQ=60)
                                               CTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTA                                                                                                        <  SRR19973083.4088515/150‑1 (MQ=60)
                                                TTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAGCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAA                                                                                                       >  SRR19973083.2095891/1‑150 (MQ=60)
                                                 TGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAC                                                                                                      >  SRR19973083.1827523/1‑150 (MQ=60)
                                                 TGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAAC                                                                                                      <  SRR19973083.3757581/150‑1 (MQ=60)
                                                   TGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTG                                                                                                    <  SRR19973083.4964446/150‑1 (MQ=60)
                                                       ATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGA                                                                                                >  SRR19973083.259455/1‑150 (MQ=60)
                                                        TTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAC                                                                                               <  SRR19973083.1063779/150‑1 (MQ=60)
                                                        TTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAC                                                                                               <  SRR19973083.3820493/150‑1 (MQ=60)
                                                        TTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAC                                                                                               <  SRR19973083.3820504/150‑1 (MQ=60)
                                                         TCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACG                                                                                              >  SRR19973083.4298138/1‑150 (MQ=60)
                                                          CAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGC                                                                                             <  SRR19973083.2398385/150‑1 (MQ=60)
                                                           AGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCG                                                                                            <  SRR19973083.4857341/150‑1 (MQ=60)
                                                             ACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGG                                                                                          >  SRR19973083.2125143/1‑150 (MQ=60)
                                                              CGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGA                                                                                         <  SRR19973083.645609/150‑1 (MQ=60)
                                                                 CCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGA                                                                                      >  SRR19973083.429426/1‑150 (MQ=60)
                                                                 CCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGA                                                                                      >  SRR19973083.2712953/1‑150 (MQ=60)
                                                                 CCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATAGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTGTAACTGTAGACGCGGGAGGA                                                                                      >  SRR19973083.429430/1‑150 (MQ=60)
                                                                  CGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAT                                                                                     <  SRR19973083.2482692/150‑1 (MQ=60)
                                                                  CGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAT                                                                                     <  SRR19973083.2482773/150‑1 (MQ=60)
                                                                      GCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAC                                                                                 <  SRR19973083.4243110/150‑1 (MQ=60)
                                                                      GCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAC                                                                                 <  SRR19973083.1182835/150‑1 (MQ=60)
                                                                         CCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCC                                                                              >  SRR19973083.581073/1‑150 (MQ=60)
                                                                           ATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACCGCGTCGTATTGAGCGTGATCACCCCGGCGCGAAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCT                                                                            >  SRR19973083.2046960/1‑150 (MQ=60)
                                                                           ATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCT                                                                            >  SRR19973083.126461/1‑150 (MQ=60)
                                                                              TTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTG                                                                         <  SRR19973083.1203500/150‑1 (MQ=60)
                                                                                TTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTG                                                                       <  SRR19973083.395915/150‑1 (MQ=60)
                                                                                TTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTG                                                                       <  SRR19973083.2842808/150‑1 (MQ=60)
                                                                                     GTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCG                                                                  >  SRR19973083.4926808/1‑150 (MQ=60)
                                                                                     GTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCG                                                                  <  SRR19973083.2359719/150‑1 (MQ=60)
                                                                                         CGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTC                                                              <  SRR19973083.4650757/150‑1 (MQ=60)
                                                                                         CGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTC                                                              >  SRR19973083.4232492/1‑150 (MQ=60)
                                                                                                GTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCG                                                       >  SRR19973083.219749/1‑150 (MQ=60)
                                                                                                GTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCG                                                       >  SRR19973083.554960/1‑150 (MQ=60)
                                                                                                GTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCG                                                       >  SRR19973083.3175012/1‑150 (MQ=60)
                                                                                                        GAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTC                                               <  SRR19973083.1525106/150‑1 (MQ=60)
                                                                                                           CCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGA                                            >  SRR19973083.3142426/1‑150 (MQ=60)
                                                                                                           CCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGA                                            >  SRR19973083.2124513/1‑150 (MQ=60)
                                                                                                            CTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGAT                                           >  SRR19973083.3959984/1‑150 (MQ=60)
                                                                                                             TGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATC                                          <  SRR19973083.3994397/150‑1 (MQ=60)
                                                                                                             TGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATC                                          <  SRR19973083.3994323/150‑1 (MQ=60)
                                                                                                             TGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATC                                          <  SRR19973083.46844/150‑1 (MQ=60)
                                                                                                              GGCAGGTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCG                                         <  SRR19973083.109097/150‑1 (MQ=60)
                                                                                                                    TGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAAC                                   <  SRR19973083.4355937/150‑1 (MQ=60)
                                                                                                                     GCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACA                                  <  SRR19973083.4018645/150‑1 (MQ=60)
                                                                                                                      CCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAA                                 >  SRR19973083.4241330/1‑150 (MQ=60)
                                                                                                                      CCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAA                                 <  SRR19973083.2621308/150‑1 (MQ=60)
                                                                                                                      CCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAA                                 >  SRR19973083.1636622/1‑150 (MQ=60)
                                                                                                                       CACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAG                                <  SRR19973083.2389910/150‑1 (MQ=60)
                                                                                                                         CCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGC                              <  SRR19973083.3541193/150‑1 (MQ=60)
                                                                                                                             CCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAA                          <  SRR19973083.4516657/150‑1 (MQ=60)
                                                                                                                             CCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAA                          <  SRR19973083.4516664/150‑1 (MQ=60)
                                                                                                                              CTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAAT                         <  SRR19973083.1048713/150‑1 (MQ=60)
                                                                                                                               TGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATA                        <  SRR19973083.812001/150‑1 (MQ=60)
                                                                                                                                GGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAA                       <  SRR19973083.1221903/150‑1 (MQ=60)
                                                                                                                                GGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAA                       <  SRR19973083.3052860/150‑1 (MQ=60)
                                                                                                                                GGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAA                       <  SRR19973083.4831809/150‑1 (MQ=60)
                                                                                                                                    CAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTT                   <  SRR19973083.2555668/150‑1 (MQ=60)
                                                                                                                                     AAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTT                  <  SRR19973083.265317/150‑1 (MQ=60)
                                                                                                                                      AACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTG                 <  SRR19973083.1212069/150‑1 (MQ=60)
                                                                                                                                       ACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGC                >  SRR19973083.720024/1‑150 (MQ=60)
                                                                                                                                        CAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCT               <  SRR19973083.4527787/150‑1 (MQ=60)
                                                                                                                                        CAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCT               <  SRR19973083.4527944/150‑1 (MQ=60)
                                                                                                                                         AGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCTCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTG              <  SRR19973083.4823348/150‑1 (MQ=60)
                                                                                                                                           CGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGA            >  SRR19973083.4598069/1‑150 (MQ=60)
                                                                                                                                              CGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAT         <  SRR19973083.4351317/150‑1 (MQ=60)
                                                                                                                                              CGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAT         <  SRR19973083.3013767/150‑1 (MQ=60)
                                                                                                                                              CGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAT         <  SRR19973083.4757001/150‑1 (MQ=60)
                                                                                                                                               GTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATA        >  SRR19973083.3148913/1‑150 (MQ=60)
                                                                                                                                                TATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAC       <  SRR19973083.3237018/150‑1 (MQ=60)
                                                                                                                                                 ATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC      >  SRR19973083.4553604/1‑150 (MQ=60)
                                                                                                                                                 ATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACC      >  SRR19973083.4553017/1‑150 (MQ=60)
                                                                                                                                                     AGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  <  SRR19973083.1177768/150‑1 (MQ=60)
                                                                                                                                                     AGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  SRR19973083.4737855/1‑150 (MQ=60)
                                                                                                                                                     AGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  SRR19973083.4737993/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCA  >  CP009273/662482‑662780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑