Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 805,678 G→T 63.5% T293T (ACG→ACT)  bioB → biotin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273805,6780GT63.5% 94.0 / 229.1 184T293T (ACG→ACT) bioBbiotin synthase
Reads supporting (aligned to +/- strand):  ref base G (25/42);  new base T (58/59);  total (83/101)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 187 total aligned reads displayed.

TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA  >  CP009273/805531‑805821
                                                                                                                                                   |                                                                                                                                               
tCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACg                                                                                                                                                 >  1:123829/1‑148 (MQ=255)
tCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACg                                                                                                                                                 >  1:3317130/1‑148 (MQ=255)
    aCCAGTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGa                                                                                                                                             <  1:473083/148‑1 (MQ=255)
     ccATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGaa                                                                                                                                            <  1:1643021/148‑1 (MQ=255)
          gCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg                                                                                                                                       <  1:2562231/148‑1 (MQ=255)
          gCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg                                                                                                                                       <  1:1728388/148‑1 (MQ=255)
          gCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCgg                                                                                                                                       <  1:2562163/148‑1 (MQ=255)
           cGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGga                                                                                                                                      <  1:3325202/148‑1 (MQ=255)
               cgcgcgGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGgaagaa                                                                                                                                  <  2:17610/148‑1 (MQ=255)
                gcgcgGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGaagaag                                                                                                                                 <  2:1033263/148‑1 (MQ=255)
                 cgcgGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACAACTCCGAATCAGGAagaaga                                                                                                                                >  1:2987167/1‑148 (MQ=255)
                     gATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATaaa                                                                                                                            >  1:1837648/1‑148 (MQ=255)
                       tCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGa                                                                                                                          >  2:2446547/1‑148 (MQ=255)
                        cATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGAc                                                                                                                         <  1:634972/148‑1 (MQ=255)
                        cATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGAc                                                                                                                         <  1:3199362/148‑1 (MQ=255)
                        cATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGAc                                                                                                                         <  1:635133/148‑1 (MQ=255)
                         atgatgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGAcc                                                                                                                        <  2:559462/148‑1 (MQ=255)
                             tgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCa                                                                                                                    <  2:3128283/148‑1 (MQ=255)
                              gCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGACGAAGATAAAGACCTGCaa                                                                                                                   >  2:3088757/1‑148 (MQ=255)
                              gCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCaa                                                                                                                   <  2:690320/148‑1 (MQ=255)
                              gCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCaa                                                                                                                   >  2:1314591/1‑148 (MQ=255)
                              gCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCaa                                                                                                                   >  2:494506/1‑148 (MQ=255)
                              gCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCaa                                                                                                                   <  2:1194877/148‑1 (MQ=255)
                               ccAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAAc                                                                                                                  >  1:1674099/1‑148 (MQ=255)
                                cAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACt                                                                                                                 >  2:42907/1‑148 (MQ=255)
                                cAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACt                                                                                                                 >  1:2822926/1‑148 (MQ=255)
                                  aCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGt                                                                                                               <  2:751016/148‑1 (MQ=255)
                                   ccTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGtt                                                                                                              <  1:1076134/148‑1 (MQ=255)
                                     tctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTcc                                                                                                            >  1:164412/1‑148 (MQ=255)
                                      ctTACGTGCGCCTTTCTGCAGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                           >  2:2231856/1‑148 (MQ=255)
                                         aCGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCaa                                                                                                        <  1:394502/148‑1 (MQ=255)
                                         aCGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCaa                                                                                                        >  2:2893611/1‑148 (MQ=255)
                                         aCGTGCGACTTTCTGGCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCaa                                                                                                        <  1:688324/148‑1 (MQ=255)
                                                 cTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGc                                                                                                >  1:3112628/1‑148 (MQ=255)
                                                 cTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTGCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGc                                                                                                >  1:2101483/1‑148 (MQ=255)
                                                   ttCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTa                                                                                              >  2:628158/1‑148 (MQ=255)
                                                      tGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAAt                                                                                           >  2:2168223/1‑148 (MQ=255)
                                                        ccGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATcc                                                                                         >  2:3132859/1‑148 (MQ=255)
                                                                   cAGATTAAAGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGAAAACTCTATTTTCTACTGTTGCAAACTGCTTACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACtt                                                                              <  2:1437696/148‑2 (MQ=255)
                                                                   cAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACtt                                                                              <  2:2832004/148‑2 (MQ=255)
                                                                   cAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTg                                                                              >  1:2674421/1‑148 (MQ=255)
                                                                   cAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTg                                                                              <  2:3209757/148‑1 (MQ=255)
                                                                    aGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGc                                                                             <  1:2235579/148‑1 (MQ=255)
                                                                     gATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGcc                                                                            >  2:619548/1‑148 (MQ=255)
                                                                        gaacgaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTg                                                                         <  2:260722/148‑1 (MQ=255)
                                                                        gaacgaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTg                                                                         <  1:2407239/148‑1 (MQ=255)
                                                                          acgaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCt                                                                       >  1:2315036/1‑148 (MQ=255)
                                                                            gaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTgg                                                                     >  2:2585642/1‑148 (MQ=255)
                                                                              acAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCa                                                                   <  1:1449219/148‑1 (MQ=255)
                                                                               cAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAg                                                                  >  2:1448989/1‑148 (MQ=255)
                                                                               cAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAg                                                                  >  1:938088/1‑148 (MQ=255)
                                                                                   cTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGa                                                              <  2:1567019/148‑1 (MQ=255)
                                                                                   cTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGa                                                              <  2:2407541/148‑1 (MQ=255)
                                                                                      aGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATaa                                                           <  2:1706750/148‑1 (MQ=255)
                                                                                       ggCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAAc                                                          >  1:1564220/1‑148 (MQ=255)
                                                                                        gCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACg                                                         >  1:1348815/1‑148 (MQ=255)
                                                                                          gATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaa                                                       <  1:3531967/148‑1 (MQ=255)
                                                                                             gtgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaacaa                                                    >  2:3248536/1‑148 (MQ=255)
                                                                                              tgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTAGCAGGGGATAACGaacaac                                                   >  2:946853/1‑148 (MQ=255)
                                                                                              tgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTAGCAGGGGATAACGaacaac                                                   >  2:443146/1‑148 (MQ=255)
                                                                                                 tttATGGCAGGCTCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAAGTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGc                                                <  2:402087/148‑1 (MQ=255)
                                                                                                     tGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACg                                            <  1:2783254/148‑1 (MQ=255)
                                                                                                       gCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTc                                          >  1:2507171/1‑148 (MQ=255)
                                                                                                       gCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTc                                          <  1:856809/148‑1 (MQ=255)
                                                                                                        cAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCt                                         <  2:2917534/148‑1 (MQ=255)
                                                                                                           gcgcAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGa                                      <  2:1837648/148‑1 (MQ=255)
                                                                                                             tcAAACTCGATTTTCTACGGTTGCAAACGGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAAc                                    <  2:2375306/147‑1 (MQ=255)
                                                                                                             gcAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAAc                                    <  2:1238335/148‑1 (MQ=255)
                                                                                                               aaaCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAg                                  <  2:517120/148‑1 (MQ=255)
                                                                                                               aaaCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAg                                  <  2:2785248/148‑1 (MQ=255)
                                                                                                               aaaCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAg                                  <  1:1897903/148‑1 (MQ=255)
                                                                                                                aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAgg                                 <  1:463483/148‑1 (MQ=255)
                                                                                                                 aCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgc                                >  2:2562192/1‑148 (MQ=255)
                                                                                                                 aCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgc                                >  2:2562207/1‑148 (MQ=255)
                                                                                                                 aCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTAGAACACgc                                >  2:3545477/1‑148 (MQ=255)
                                                                                                                  cTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcg                               <  1:619548/148‑1 (MQ=255)
                                                                                                                  cTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcg                               <  2:845235/148‑1 (MQ=255)
                                                                                                                   tCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcgc                              <  1:2125126/148‑1 (MQ=255)
                                                                                                                    cGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCt                             >  2:3446027/1‑148 (MQ=255)
                                                                                                                     gATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtg                            <  1:3433977/148‑1 (MQ=255)
                                                                                                                       ttttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgat                          >  1:512252/1‑148 (MQ=255)
                                                                                                                       ttttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgat                          <  2:1674099/148‑1 (MQ=255)
                                                                                                                        tttCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatg                         <  1:727294/148‑1 (MQ=255)
                                                                                                                        tttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatg                         >  2:2058160/1‑148 (MQ=255)
                                                                                                                         ttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatga                        <  1:2168223/148‑1 (MQ=255)
                                                                                                                          tCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAc                       >  2:2268765/1‑148 (MQ=255)
                                                                                                                            tACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAccc                     <  2:517390/148‑1 (MQ=255)
                                                                                                                             aCGGTTGCAAACTGCTGCCCACTCCGAATCCGGAAGTAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAcccc                    <  1:1128305/148‑1 (MQ=255)
                                                                                                                             aCGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAcccc                    >  2:387566/1‑148 (MQ=255)
                                                                                                                                    cAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCCACGTCTTGAACAGGCGCTGATGACCCCGGACAcc             >  2:1855241/1‑148 (MQ=255)
                                                                                                                                    cAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACAcc             >  2:2373061/1‑148 (MQ=255)
                                                                                                                                      aaCTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcga           >  1:1190533/1‑148 (MQ=255)
                                                                                                                                       aCTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgac          >  2:3145518/1‑148 (MQ=255)
                                                                                                                                       aCTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgac          >  2:3145493/1‑148 (MQ=255)
                                                                                                                                       aCTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgac          >  2:1002836/1‑148 (MQ=255)
                                                                                                                                           ctgACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAat      <  2:254095/148‑1 (MQ=255)
                                                                                                                                              aCCACTCCGAATCCAGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATAtt   >  2:1263244/1‑148 (MQ=255)
                                                                                                                                              aCCACGCCGAATCCGGAAGAAGATATAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATAtt   <  1:256959/148‑1 (MQ=255)
                                                                                                                                               ccACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTa  >  1:3295069/1‑148 (MQ=255)
                                                                                                                                               ccACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTa  >  1:3489978/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA  >  CP009273/805531‑805821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAC  >  CP009273/805529‑805825
                                                                                                                                                     |                                                                                                                                                   
ATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACT                                                                                                                                                     >  SRR19973066.119484/1‑150 (MQ=60)
ATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACG                                                                                                                                                     >  SRR19973066.3338833/1‑150 (MQ=60)
ATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACG                                                                                                                                                     >  SRR19973066.3339152/1‑150 (MQ=60)
ATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACG                                                                                                                                                     >  SRR19973066.125070/1‑150 (MQ=60)
  TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGANCGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCC                                                                                                                                                   <  SRR19973066.1824677/150‑1 (MQ=60)
  TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCC                                                                                                                                                   <  SRR19973066.2016445/150‑1 (MQ=60)
   CGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCG                                                                                                                                                  <  SRR19973066.2584632/150‑1 (MQ=60)
      ACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAAT                                                                                                                                               <  SRR19973066.477073/150‑1 (MQ=60)
      ACCAGTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAAT                                                                                                                                               <  SRR19973066.476666/150‑1 (MQ=60)
       CCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATC                                                                                                                                              <  SRR19973066.1654977/150‑1 (MQ=60)
        CATTGCGGTCGCGCGGATCATTATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGGGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGACTCC                                                                                                                                             >  SRR19973066.474128/1‑150 (MQ=60)
           TGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGA                                                                                                                                          >  SRR19973066.783267/1‑150 (MQ=60)
           TGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGA                                                                                                                                          <  SRR19973066.2171038/150‑1 (MQ=60)
            GCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAA                                                                                                                                         <  SRR19973066.2579623/150‑1 (MQ=60)
            GCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAA                                                                                                                                         <  SRR19973066.1740882/150‑1 (MQ=60)
            GCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAA                                                                                                                                         <  SRR19973066.2579555/150‑1 (MQ=60)
            GCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAA                                                                                                                                         <  SRR19973066.2579577/150‑1 (MQ=60)
             CGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAG                                                                                                                                        <  SRR19973066.3347295/150‑1 (MQ=60)
             CGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAG                                                                                                                                        <  SRR19973066.1568623/150‑1 (MQ=60)
             CGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAG                                                                                                                                        <  SRR19973066.3349269/150‑1 (MQ=60)
                 CGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACAACTCCGAATCAGGAAGAAGA                                                                                                                                    >  SRR19973066.3006921/1‑150 (MQ=60)
                     CGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAA                                                                                                                                >  SRR19973066.1850775/1‑150 (MQ=60)
                     CGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAA                                                                                                                                <  SRR19973066.2669499/150‑1 (MQ=60)
                         TCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACC                                                                                                                            <  SRR19973066.1244075/150‑1 (MQ=60)
                          CATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCT                                                                                                                           <  SRR19973066.241392/150‑1 (MQ=60)
                          CATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCT                                                                                                                           <  SRR19973066.639704/150‑1 (MQ=60)
                          CATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCT                                                                                                                           <  SRR19973066.3220355/150‑1 (MQ=60)
                          CATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCT                                                                                                                           <  SRR19973066.3220606/150‑1 (MQ=60)
                          CATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCT                                                                                                                           <  SRR19973066.639543/150‑1 (MQ=60)
                          CATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCT                                                                                                                           <  SRR19973066.639579/150‑1 (MQ=60)
                           ATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTG                                                                                                                          <  SRR19973066.2915343/150‑1 (MQ=60)
                               TGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGACTCCGGAAGAAGATAAAGACCTGCAAC                                                                                                                      >  SRR19973066.1686296/1‑150 (MQ=60)
                                GCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACT                                                                                                                     >  SRR19973066.2841897/1‑150 (MQ=60)
                                  CAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGT                                                                                                                   >  SRR19973066.3084344/1‑150 (MQ=60)
                                  CAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGT                                                                                                                   >  SRR19973066.2554332/1‑150 (MQ=60)
                                   AACCTCTTCCGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTT                                                                                                                  <  SRR19973066.2804085/150‑1 (MQ=60)
                                   AACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTT                                                                                                                  <  SRR19973066.1722145/150‑1 (MQ=60)
                                     CCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCC                                                                                                                <  SRR19973066.1084006/150‑1 (MQ=60)
                                     CCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCC                                                                                                                >  SRR19973066.165899/1‑150 (MQ=60)
                                     CATCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCC                                                                                                                >  SRR19973066.166050/1‑150 (MQ=60)
                                           ACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAAC                                                                                                          <  SRR19973066.397722/150‑1 (MQ=60)
                                           ACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAAC                                                                                                          <  SRR19973066.397609/150‑1 (MQ=60)
                                           ACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAAC                                                                                                          <  SRR19973066.3276934/150‑1 (MQ=60)
                                           ACGTGCGACTTTCTGGCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAAC                                                                                                          <  SRR19973066.693383/150‑1 (MQ=60)
                                                 GCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAATGACCTGCAACTGTTCCGCAAACTGGGGC                                                                                                    <  SRR19973066.1870411/150‑1 (MQ=60)
                                                 GCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGC                                                                                                    <  SRR19973066.1792043/150‑1 (MQ=60)
                                                 GCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGC                                                                                                    >  SRR19973066.3133416/1‑150 (MQ=60)
                                                 GCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTGCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGC                                                                                                    >  SRR19973066.2115884/1‑150 (MQ=60)
                                                 GCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTGCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGC                                                                                                    >  SRR19973066.2115956/1‑150 (MQ=60)
                                                 GCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTGCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGC                                                                                                    >  SRR19973066.2117050/1‑150 (MQ=60)
                                                  CCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCT                                                                                                   >  SRR19973066.251074/1‑150 (MQ=60)
                                                      TCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAAT                                                                                               <  SRR19973066.1186977/150‑1 (MQ=60)
                                                       CTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATC                                                                                              <  SRR19973066.1255219/150‑1 (MQ=60)
                                                                   AGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTG                                                                                  >  SRR19973066.2692287/1‑150 (MQ=60)
                                                                      AGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCG                                                                               <  SRR19973066.2251002/150‑1 (MQ=60)
                                                                          GAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCT                                                                           >  SRR19973066.2330873/1‑150 (MQ=60)
                                                                          GAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCT                                                                           <  SRR19973066.2423527/150‑1 (MQ=60)
                                                                               AACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAG                                                                      >  SRR19973066.944913/1‑150 (MQ=60)
                                                                               AACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAG                                                                      >  SRR19973066.944894/1‑150 (MQ=60)
                                                                                ACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGG                                                                     <  SRR19973066.1459513/150‑1 (MQ=60)
                                                                                      TCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAA                                                               <  SRR19973066.3550941/150‑1 (MQ=60)
                                                                                       CAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAAC                                                              >  SRR19973066.1575487/1‑150 (MQ=60)
                                                                                        AGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACG                                                             >  SRR19973066.1358595/1‑150 (MQ=60)
                                                                                            GATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACA                                                         >  SRR19973066.1360714/1‑150 (MQ=60)
                                                                                            GATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACA                                                         >  SRR19973066.360386/1‑150 (MQ=60)
                                                                                            GATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACA                                                         <  SRR19973066.3555453/150‑1 (MQ=60)
                                                                                                     TATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACG                                                <  SRR19973066.2286382/150‑1 (MQ=60)
                                                                                                       TGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTC                                              <  SRR19973066.2801994/150‑1 (MQ=60)
                                                                                                       TGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTC                                              >  SRR19973066.2524245/1‑150 (MQ=60)
                                                                                                         GCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTT                                            <  SRR19973066.862987/150‑1 (MQ=60)
                                                                                                                 AAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGC                                    <  SRR19973066.1911442/150‑1 (MQ=60)
                                                                                                                  AACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCG                                   <  SRR19973066.467025/150‑1 (MQ=60)
                                                                                                                    CTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCT                                 <  SRR19973066.623932/150‑1 (MQ=60)
                                                                                                                    CTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCT                                 <  SRR19973066.623949/150‑1 (MQ=60)
                                                                                                                     TCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTG                                <  SRR19973066.2139842/150‑1 (MQ=60)
                                                                                                                       GATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGAT                              <  SRR19973066.3456951/150‑1 (MQ=60)
                                                                                                                       GATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGAT                              >  SRR19973066.516037/1‑150 (MQ=60)
                                                                                                                          TTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAC                           <  SRR19973066.732574/150‑1 (MQ=60)
                                                                                                                          TTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAC                           <  SRR19973066.732682/150‑1 (MQ=60)
                                                                                                                           TTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACC                          <  SRR19973066.640897/150‑1 (MQ=60)
                                                                                                                           TTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACC                          <  SRR19973066.1644949/150‑1 (MQ=60)
                                                                                                                           TTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACC                          <  SRR19973066.2183281/150‑1 (MQ=60)
                                                                                                                               ACGGTTGCAAACTGCTGCCCACTCCGAATCCGGAAGTAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGG                      <  SRR19973066.1136409/150‑1 (MQ=60)
                                                                                                                                      CAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGA               >  SRR19973066.3509340/1‑150 (MQ=60)
                                                                                                                                      CAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGA               >  SRR19973066.1198873/1‑150 (MQ=60)
                                                                                                                                             CTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATAT        <  SRR19973066.970460/150‑1 (MQ=60)
                                                                                                                                               GACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA      >  SRR19973066.3316827/1‑150 (MQ=60)
                                                                                                                                               GACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA      >  SRR19973066.3513262/1‑150 (MQ=60)
                                                                                                                                                ACCACGCCGAATCCGGAAGAAGATATAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTAC     <  SRR19973066.258905/150‑1 (MQ=60)
                                                                                                                                                   ACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAC  <  SRR19973066.2463212/150‑1 (MQ=60)
                                                                                                                                                   ACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAC  >  SRR19973066.2383166/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
ATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAC  >  CP009273/805529‑805825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑