Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 809,212 G→T 5.6% M77I (ATG→ATT)  uvrB → excinulease of nucleotide excision repair, DNA damage recognition component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273809,2120GT 5.6% 1098.9 / 26.3 284M77I (ATG→ATT) uvrBexcinulease of nucleotide excision repair, DNA damage recognition component
Reads supporting (aligned to +/- strand):  ref base G (105/163);  new base T (9/7);  total (114/170)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 287 total aligned reads displayed.

CTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCAC  >  CP009273/809063‑809361
                                                                                                                                                     |                                                                                                                                                     
cTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATg                                                                                                                                                       >  2:4223800/1‑150 (MQ=255)
 tGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGa                                                                                                                                                      >  1:2208624/1‑150 (MQ=255)
    aaGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAag                                                                                                                                                   >  1:1934247/1‑150 (MQ=255)
        tGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGtt                                                                                                                                               <  2:1710578/150‑1 (MQ=255)
          gCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGttct                                                                                                                                             <  2:3842607/150‑1 (MQ=255)
           ccTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGttctt                                                                                                                                            >  1:1610957/1‑150 (MQ=255)
            cTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATTAAAGAGttcttc                                                                                                                                           <  2:4579737/150‑1 (MQ=255)
            cTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGttcttc                                                                                                                                           <  2:2398527/150‑1 (MQ=255)
             tGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTcc                                                                                                                                          >  1:909147/1‑150 (MQ=255)
               gcgcACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCg                                                                                                                                        <  2:3124159/150‑1 (MQ=255)
                cgcACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAATCCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCTTGGTACTTGCGCCCAACAAAACGCTGGCGGCCCTGCTGTATGGCGAAATGAAAGAGTTCTTCCCgt                                                                                                                                       <  2:2557000/150‑2 (MQ=255)
                    ccAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGaaaa                                                                                                                                   >  1:3421896/1‑150 (MQ=255)
                        aCGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAAcgcg                                                                                                                               <  2:1491947/150‑1 (MQ=255)
                         cGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCgg                                                                                                                              >  1:1786271/1‑150 (MQ=255)
                           ttACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTg                                                                                                                            >  2:4421076/1‑150 (MQ=255)
                             aCTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGa                                                                                                                          <  1:1005282/150‑1 (MQ=255)
                               ttGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAat                                                                                                                        >  1:1929833/1‑150 (MQ=255)
                                     tGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCg                                                                                                                  <  1:3546010/150‑1 (MQ=255)
                                      gACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGt                                                                                                                 >  2:1479265/1‑150 (MQ=255)
                                       aCTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGtt                                                                                                                <  1:1760925/150‑1 (MQ=255)
                                        cTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGttt                                                                                                               >  2:3393047/1‑150 (MQ=255)
                                        cTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGttt                                                                                                               <  1:4068683/150‑1 (MQ=255)
                                            cTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCcta                                                                                                           <  1:2895773/150‑1 (MQ=255)
                                              cAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCctact                                                                                                         <  2:2618924/150‑1 (MQ=255)
                                              cAGGGAAAACCTTCACCAATGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCctact                                                                                                         <  1:3658385/150‑1 (MQ=255)
                                               aGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAACCGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCctacta                                                                                                        <  1:3381021/150‑1 (MQ=255)
                                                gggAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCtactac                                                                                                       <  1:1049385/150‑1 (MQ=255)
                                                gggAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCtaatac                                                                                                       >  2:905836/1‑150 (MQ=255)
                                                   aaaaCCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGac                                                                                                    <  2:2323829/150‑1 (MQ=255)
                                                   aaaaCCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGac                                                                                                    >  2:4293233/1‑150 (MQ=255)
                                                     aaCCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATTAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGacta                                                                                                  >  1:2451559/1‑150 (MQ=255)
                                                       ccTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCGTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGactact                                                                                                <  2:4139127/150‑1 (MQ=255)
                                                        cTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGActacta                                                                                               <  2:4006455/150‑1 (MQ=255)
                                                        cTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGActacta                                                                                               >  1:2710260/1‑150 (MQ=255)
                                                         ttCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTAt                                                                                              <  2:3246252/150‑1 (MQ=255)
                                                          tCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATc                                                                                             >  2:4505197/1‑150 (MQ=255)
                                                           cACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCa                                                                                            >  1:2358492/1‑150 (MQ=255)
                                                           cACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCa                                                                                            <  2:4271529/150‑1 (MQ=255)
                                                            aCCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAg                                                                                           <  1:663533/150‑1 (MQ=255)
                                                                      aTGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCt                                                                                 >  1:3295856/1‑150 (MQ=255)
                                                                      aTGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACACGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCt                                                                                 >  2:3009656/1‑150 (MQ=255)
                                                                        gTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATTAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTAt                                                                               <  1:2424454/150‑1 (MQ=255)
                                                                        gTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTAt                                                                               <  2:187962/150‑1 (MQ=255)
                                                                        gTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTAt                                                                               >  2:585639/1‑150 (MQ=255)
                                                                          cATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGATTCCTACTACGACTACTATCAGCCGGAAGCCTAGGt                                                                             >  2:652164/1‑150 (MQ=255)
                                                                            ttGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTAc                                                                           <  1:2929270/150‑1 (MQ=255)
                                                                             tGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTAcc                                                                          <  2:82772/150‑1 (MQ=255)
                                                                               cTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGa                                                                        <  1:598950/150‑1 (MQ=255)
                                                                                  aCCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGtt                                                                     <  1:4360402/150‑1 (MQ=255)
                                                                                   ccTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTc                                                                    <  2:4478981/150‑1 (MQ=255)
                                                                                    cTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTcc                                                                   >  2:2219394/1‑150 (MQ=255)
                                                                                     ttCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCg                                                                  <  2:1013230/150‑1 (MQ=255)
                                                                                     ttCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCg                                                                  <  1:842731/150‑1 (MQ=255)
                                                                                      tCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGa                                                                 >  2:4371839/1‑150 (MQ=255)
                                                                                       cAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGac                                                                >  1:3733364/1‑150 (MQ=255)
                                                                                        aGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGGTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGaca                                                               <  1:1677829/150‑1 (MQ=255)
                                                                                          cgcCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACt                                                             <  2:1727375/150‑1 (MQ=255)
                                                                                           gcCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACtt                                                            >  2:2845519/1‑150 (MQ=255)
                                                                                            cccAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACttt                                                           <  1:2096813/150‑1 (MQ=255)
                                                                                            cccAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACttt                                                           >  2:2499451/1‑150 (MQ=255)
                                                                                              cAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCa                                                         >  1:3825910/1‑150 (MQ=255)
                                                                                              cAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCa                                                         <  2:1314008/150‑1 (MQ=255)
                                                                                                aCCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCAtt                                                       <  1:3049242/150‑1 (MQ=255)
                                                                                                      gTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGaaa                                                 <  1:2831638/150‑1 (MQ=255)
                                                                                                      gTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGaaa                                                 <  2:433182/150‑1 (MQ=255)
                                                                                                      gTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGaaa                                                 <  1:569882/150‑1 (MQ=255)
                                                                                                       tACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAg                                                >  2:1132236/1‑150 (MQ=255)
                                                                                                        aCTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATTAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGa                                               >  1:49536/1‑150 (MQ=255)
                                                                                                          ttGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTANCGAGTTCCGACACTTTCATTGAGAAAGATg                                             <  1:4279808/150‑1 (MQ=255)
                                                                                                          ttGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATg                                             <  1:1269574/150‑1 (MQ=255)
                                                                                                             cgcCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATTAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCt                                          >  1:1458664/1‑150 (MQ=255)
                                                                                                             cgcCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCt                                          <  2:3776543/150‑1 (MQ=255)
                                                                                                              gcCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTc                                         >  2:2391456/1‑150 (MQ=255)
                                                                                                                ccAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCgg                                       >  2:3347803/1‑150 (MQ=255)
                                                                                                                 caacaaCTCGCTGGCGGCCCTGCTGTATGGCGAAATGAAAGAGGTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGt                                      <  2:476400/150‑1 (MQ=255)
                                                                                                                 caacaaAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGt                                      >  1:4382826/1‑150 (MQ=255)
                                                                                                                 caacaaAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGt                                      <  2:1944901/150‑1 (MQ=255)
                                                                                                                 caacaaAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGt                                      <  2:3680548/150‑1 (MQ=255)
                                                                                                                     aacaCGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAAc                                  <  2:735688/147‑1 (MQ=255)
                                                                                                                     aaaaCGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAAc                                  >  2:1586747/1‑150 (MQ=255)
                                                                                                                     aaaaCGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAAc                                  <  1:137316/150‑1 (MQ=255)
                                                                                                                      aaaCGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACg                                 >  1:2293318/1‑150 (MQ=255)
                                                                                                                       aaCGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGa                                <  2:1719152/150‑1 (MQ=255)
                                                                                                                           cTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACat                            >  1:401544/1‑150 (MQ=255)
                                                                                                                            tGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACata                           <  2:1368768/150‑1 (MQ=255)
                                                                                                                               cggcCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTCTCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTg                        <  2:564593/150‑1 (MQ=255)
                                                                                                                                  cccAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGc                     <  2:1267545/150‑1 (MQ=255)
                                                                                                                                     aGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGa                  >  1:2538626/1‑150 (MQ=255)
                                                                                                                                      gCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGAt                 <  1:1995916/150‑1 (MQ=255)
                                                                                                                                      gCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGAt                 <  2:4577275/150‑1 (MQ=255)
                                                                                                                                       cTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATg                <  1:4549989/150‑1 (MQ=255)
                                                                                                                                        tGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGc               >  2:4471375/1‑150 (MQ=255)
                                                                                                                                        tGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGc               >  2:3503358/1‑150 (MQ=255)
                                                                                                                                           aTGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGtt            >  1:4184574/1‑150 (MQ=255)
                                                                                                                                            tGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGttt           >  1:2271222/1‑150 (MQ=255)
                                                                                                                                            tGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGttt           <  2:3212184/150‑1 (MQ=255)
                                                                                                                                               cGAAATTAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTc        >  1:2670265/1‑150 (MQ=255)
                                                                                                                                               cGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTc        <  1:4617501/150‑1 (MQ=255)
                                                                                                                                                  aaTGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGc     >  2:753789/1‑150 (MQ=255)
                                                                                                                                                   aTGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGcc    >  1:2751536/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGAAGATGGCCTGGCGCACCAGACGTTACTTGGCGTGACTGGCTCAGGGAAAACCTTCACCATTGCCAATGTCATTGCTGACCTTCAGCGCCCAACCATGGTACTTGCGCCCAACAAAACGCTGGCGGCCCAGCTGTATGGCGAAATGAAAGAGTTCTTCCCGGAAAACGCGGTGGAATATTTCGTTTCCTACTACGACTACTATCAGCCGGAAGCCTATGTACCGAGTTCCGACACTTTCATTGAGAAAGATGCCTCGGTTAACGAACATATTGAGCAGATGCGTTTGTCCGCCAC  >  CP009273/809063‑809361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A