Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 805,678 G→T 100% T293T (ACG→ACT)  bioB → biotin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273805,6780GT98.4% 603.2 / 0.8 188T293T (ACG→ACT) bioBbiotin synthase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (100/85);  total (102/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

GCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTAC  >  CP009273/805533‑805822
                                                                                                                                                 |                                                                                                                                                
gCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTcc                                                                                                                                                >  2:1552476/1‑148 (MQ=255)
  aCCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGa                                                                                                                                              <  2:1718297/148‑1 (MQ=255)
   ccATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGaa                                                                                                                                             <  1:3018651/148‑1 (MQ=255)
     aTTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGACTc                                                                                                                                           >  2:3316358/1‑148 (MQ=255)
     aTTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATc                                                                                                                                           >  2:1810431/1‑148 (MQ=255)
      ttGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATcc                                                                                                                                          >  2:258450/1‑148 (MQ=255)
       tGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCg                                                                                                                                         <  1:1033277/148‑1 (MQ=255)
        gCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCgg                                                                                                                                        <  1:1087594/148‑1 (MQ=255)
            tCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGgaaga                                                                                                                                    <  2:919881/148‑1 (MQ=255)
               cgcgGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAagaaga                                                                                                                                 <  2:878889/148‑1 (MQ=255)
                gcgGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGAt                                                                                                                                <  2:476309/148‑1 (MQ=255)
                 cgGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATa                                                                                                                               <  1:2167223/148‑1 (MQ=255)
                   gATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATaaa                                                                                                                             <  2:1723989/148‑1 (MQ=255)
                   gATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATaaa                                                                                                                             <  2:420505/148‑1 (MQ=255)
                   gATCATGATGCCAACCTCTTACGTGCGCCTTACTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATaaa                                                                                                                             >  2:789026/1‑148 (MQ=255)
                     tCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGa                                                                                                                           >  1:981930/1‑148 (MQ=255)
                      cATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGAc                                                                                                                          <  1:1706165/148‑1 (MQ=255)
                      cATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGAc                                                                                                                          >  2:2312088/1‑148 (MQ=255)
                       atgatgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGAcc                                                                                                                         <  1:2005883/148‑1 (MQ=255)
                        tgatgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCt                                                                                                                        >  2:681664/1‑148 (MQ=255)
                            gCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCATATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCaa                                                                                                                    <  1:2120437/148‑1 (MQ=255)
                             ccAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAAc                                                                                                                   >  2:1343265/1‑148 (MQ=255)
                                  ctctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTc                                                                                                              <  1:184209/148‑1 (MQ=255)
                                  ctctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTc                                                                                                              >  1:1777878/1‑148 (MQ=255)
                                   tctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTcc                                                                                                             >  1:1594168/1‑148 (MQ=255)
                                    ctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                            >  2:269335/1‑148 (MQ=255)
                                    ctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                            >  2:688227/1‑148 (MQ=255)
                                    ctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                            >  1:3106692/1‑148 (MQ=255)
                                    ctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                            <  2:67736/148‑1 (MQ=255)
                                      tACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCa                                                                                                          >  1:1993469/1‑148 (MQ=255)
                                           gcgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTg                                                                                                     <  2:3111666/148‑1 (MQ=255)
                                             gcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTggg                                                                                                   <  2:3264512/148‑1 (MQ=255)
                                               cTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGc                                                                                                 >  2:824948/1‑148 (MQ=255)
                                               cTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGc                                                                                                 >  1:1666687/1‑148 (MQ=255)
                                                  tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTaa                                                                                              >  1:813322/1‑148 (MQ=255)
                                                     gCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATc                                                                                           <  2:2197875/148‑1 (MQ=255)
                                                     gCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATc                                                                                           <  2:2197920/148‑1 (MQ=255)
                                                        ggACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCgc                                                                                        <  1:260523/148‑1 (MQ=255)
                                                         gACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCgca                                                                                       >  1:2194992/1‑148 (MQ=255)
                                                         gACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCgca                                                                                       <  1:3006402/148‑1 (MQ=255)
                                                               aGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAAc                                                                                 >  1:2926202/1‑148 (MQ=255)
                                                                gCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACt                                                                                <  2:894560/148‑1 (MQ=255)
                                                                gCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACt                                                                                >  2:2799208/1‑148 (MQ=255)
                                                                 cAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTg                                                                               <  2:1141798/148‑1 (MQ=255)
                                                                 cAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTg                                                                               >  1:919021/1‑148 (MQ=255)
                                                                   gATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGcc                                                                             <  1:476237/148‑1 (MQ=255)
                                                                   gATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGcc                                                                             >  1:689106/1‑148 (MQ=255)
                                                                    aTGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCg                                                                            >  2:1957260/1‑148 (MQ=255)
                                                                    aTGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCg                                                                            <  1:1237031/148‑1 (MQ=255)
                                                                    aTGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCg                                                                            >  2:2381313/1‑148 (MQ=255)
                                                                      gaacgaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTg                                                                          <  1:2312088/148‑1 (MQ=255)
                                                                      gaacgaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTg                                                                          <  2:1913052/148‑1 (MQ=255)
                                                                        acgaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCt                                                                        >  1:1195081/1‑148 (MQ=255)
                                                                             cAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAg                                                                   >  2:463845/1‑148 (MQ=255)
                                                                               gACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGACTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAggg                                                                 >  1:1973262/1‑148 (MQ=255)
                                                                               gACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAggg                                                                 <  2:3443859/148‑1 (MQ=255)
                                                                                  tCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGAt                                                              <  2:3187341/148‑1 (MQ=255)
                                                                                   cAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATa                                                             >  2:2923100/1‑148 (MQ=255)
                                                                                   cAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATa                                                             <  1:1981870/148‑1 (MQ=255)
                                                                                     ggCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAAc                                                           >  1:114058/1‑148 (MQ=255)
                                                                                         aTGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaac                                                       >  1:560647/1‑148 (MQ=255)
                                                                                          tgtgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaaca                                                      >  2:2458587/1‑148 (MQ=255)
                                                                                           gtgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaacaa                                                     >  1:623169/1‑148 (MQ=255)
                                                                                           gtgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaacaa                                                     >  2:1358064/1‑148 (MQ=255)
                                                                                                    ggCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGt                                            >  2:1277289/1‑148 (MQ=255)
                                                                                                    ggCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGt                                            >  1:2183399/1‑148 (MQ=255)
                                                                                                     gCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTc                                           <  1:1427764/148‑1 (MQ=255)
                                                                                                      cAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCt                                          <  2:2011073/148‑1 (MQ=255)
                                                                                                              aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAgg                                  >  2:562482/1‑148 (MQ=255)
                                                                                                              aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAgg                                  <  2:1651337/148‑1 (MQ=255)
                                                                                                              aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAgg                                  <  2:3106692/148‑1 (MQ=255)
                                                                                                              aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAgg                                  >  2:2651344/1‑148 (MQ=255)
                                                                                                                ctctATTTTCTTCGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGATAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAAAAACAGCAACGTCTTGAACAGgcg                                <  1:3318992/148‑1 (MQ=255)
                                                                                                                cTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcg                                <  1:1580891/148‑1 (MQ=255)
                                                                                                                cTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcg                                >  1:1496721/1‑148 (MQ=255)
                                                                                                                  cGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCt                              <  2:1555314/148‑1 (MQ=255)
                                                                                                                  cGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCt                              >  2:1270106/1‑148 (MQ=255)
                                                                                                                   gATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtg                             <  1:2415031/148‑1 (MQ=255)
                                                                                                                   gATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtg                             <  2:3120974/148‑1 (MQ=255)
                                                                                                                    aTTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtga                            >  1:2502468/1‑148 (MQ=255)
                                                                                                                    aTTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtga                            >  1:2502582/1‑148 (MQ=255)
                                                                                                                    aTTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtga                            >  2:3405657/1‑148 (MQ=255)
                                                                                                                     ttttCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgat                           >  2:1507070/1‑148 (MQ=255)
                                                                                                                     ttttCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgat                           >  2:911978/1‑148 (MQ=255)
                                                                                                                       ttCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatga                         <  2:2848225/148‑1 (MQ=255)
                                                                                                                       ttCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatga                         >  2:671810/1‑148 (MQ=255)
                                                                                                                           aCGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAcccc                     >  1:2786995/1‑148 (MQ=255)
                                                                                                                            cGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCg                    >  1:3251923/1‑148 (MQ=255)
                                                                                                                               ttGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGac                 <  1:331302/148‑1 (MQ=255)
                                                                                                                               ttGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGac                 >  2:1245384/1‑148 (MQ=255)
                                                                                                                                tGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGaca                <  2:1208992/148‑1 (MQ=255)
                                                                                                                                     aCTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgac           >  2:2084574/1‑148 (MQ=255)
                                                                                                                                     aCTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgac           >  1:2474451/1‑148 (MQ=255)
                                                                                                                                      ctgctgACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgacg          >  1:756966/1‑148 (MQ=255)
                                                                                                                                        gctgACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgacgac        >  2:2442172/1‑147 (MQ=255)
                                                                                                                                         ctgACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAat       >  1:658643/1‑148 (MQ=255)
                                                                                                                                          tgACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAata      <  1:2044939/148‑1 (MQ=255)
                                                                                                                                           gACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAatat     <  1:3441435/148‑1 (MQ=255)
                                                                                                                                            aCCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGACTAtt    >  2:1800978/1‑148 (MQ=255)
                                                                                                                                              cACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTAc  <  1:3145401/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTAC  >  CP009273/805533‑805822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGC  >  CP009273/805531‑805827
                                                                                                                                                   |                                                                                                                                                     
TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCC                                                                                                                                                     <  SRR19973065.3284461/150‑1 (MQ=60)
     CCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATC                                                                                                                                                <  SRR19973065.3037145/150‑1 (MQ=60)
      CATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCC                                                                                                                                               >  SRR19973065.1216783/1‑150 (MQ=60)
       ATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCG                                                                                                                                              <  SRR19973065.496232/150‑1 (MQ=60)
         TGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGA                                                                                                                                            <  SRR19973065.1040116/150‑1 (MQ=60)
          GCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAA                                                                                                                                           <  SRR19973065.1094763/150‑1 (MQ=60)
            GGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGA                                                                                                                                         >  SRR19973065.1824868/1‑150 (MQ=60)
               CGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGA                                                                                                                                      <  SRR19973065.1990169/150‑1 (MQ=60)
                   CGGATCATGATGCCAACCTCTTCCGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAA                                                                                                                                  <  SRR19973065.2181146/150‑1 (MQ=60)
                     GATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGA                                                                                                                                >  SRR19973065.988447/1‑150 (MQ=60)
                     GATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGA                                                                                                                                >  SRR19973065.987532/1‑150 (MQ=60)
                       TCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACC                                                                                                                              <  SRR19973065.810155/150‑1 (MQ=60)
                        CATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCT                                                                                                                             <  SRR19973065.1717522/150‑1 (MQ=60)
                         ATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTG                                                                                                                            <  SRR19973065.2018843/150‑1 (MQ=60)
                              GCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCATATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACT                                                                                                                       <  SRR19973065.2134086/150‑1 (MQ=60)
                              GCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACT                                                                                                                       <  SRR19973065.1740101/150‑1 (MQ=60)
                                 AACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTT                                                                                                                    >  SRR19973065.3289236/1‑150 (MQ=60)
                                  ACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTC                                                                                                                   >  SRR19973065.1789496/1‑150 (MQ=60)
                                   CCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCC                                                                                                                  >  SRR19973065.1604716/1‑150 (MQ=60)
                                   CCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCC                                                                                                                  >  SRR19973065.1604815/1‑150 (MQ=60)
                                    CTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCG                                                                                                                 <  SRR19973065.185662/150‑1 (MQ=60)
                                    CTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCG                                                                                                                 >  SRR19973065.1368747/1‑150 (MQ=60)
                                    CTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCG                                                                                                                 <  SRR19973065.2184580/150‑1 (MQ=60)
                                    CTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCG                                                                                                                 <  SRR19973065.1970909/150‑1 (MQ=60)
                                    CTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCG                                                                                                                 >  SRR19973065.3125646/1‑150 (MQ=60)
                                      CNTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCA                                                                                                               >  SRR19973065.2006391/1‑150 (MQ=60)
                                            TGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGG                                                                                                         <  SRR19973065.3138300/150‑1 (MQ=60)
                                               GCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGC                                                                                                      >  SRR19973065.1677840/1‑150 (MQ=60)
                                                  TTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAA                                                                                                   >  SRR19973065.818685/1‑150 (MQ=60)
                                                         CGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCA                                                                                            >  SRR19973065.2209060/1‑150 (MQ=60)
                                                          GGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAG                                                                                           <  SRR19973065.260989/150‑1 (MQ=60)
                                                          GGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAG                                                                                           <  SRR19973065.262321/150‑1 (MQ=60)
                                                           GACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGC                                                                                          <  SRR19973065.3025899/150‑1 (MQ=60)
                                                           GACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGC                                                                                          <  SRR19973065.3024816/150‑1 (MQ=60)
                                                               CGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAAC                                                                                      >  SRR19973065.2943977/1‑150 (MQ=60)
                                                                 AGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTG                                                                                    >  SRR19973065.925038/1‑150 (MQ=60)
                                                                   CAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCC                                                                                  >  SRR19973065.693689/1‑150 (MQ=60)
                                                                     GATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGT                                                                                <  SRR19973065.479485/150‑1 (MQ=60)
                                                                      ATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTG                                                                               <  SRR19973065.1245145/150‑1 (MQ=60)
                                                                        GAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCT                                                                             <  SRR19973065.2326600/150‑1 (MQ=60)
                                                                        GAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCT                                                                             >  SRR19973065.1202174/1‑150 (MQ=60)
                                                                        GAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCT                                                                             >  SRR19973065.1202683/1‑150 (MQ=60)
                                                                             AACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAG                                                                        <  SRR19973065.2001549/150‑1 (MQ=60)
                                                                               CAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGACTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGG                                                                      >  SRR19973065.1986114/1‑150 (MQ=60)
                                                                                AGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGG                                                                     >  SRR19973065.2977464/1‑150 (MQ=60)
                                                                                AGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGG                                                                     >  SRR19973065.2933508/1‑150 (MQ=60)
                                                                                AGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGG                                                                     >  SRR19973065.3097022/1‑150 (MQ=60)
                                                                                    TCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAA                                                                 <  SRR19973065.2206176/150‑1 (MQ=60)
                                                                                     CAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAAC                                                                >  SRR19973065.114728/1‑150 (MQ=60)
                                                                                     CAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAAC                                                                <  SRR19973065.1994753/150‑1 (MQ=60)
                                                                                     CAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAAC                                                                >  SRR19973065.115070/1‑150 (MQ=60)
                                                                                         CGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAAC                                                            >  SRR19973065.564216/1‑150 (MQ=60)
                                                                                          GATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACA                                                           <  SRR19973065.2079585/150‑1 (MQ=60)
                                                                                          GATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACA                                                           >  SRR19973065.625116/1‑150 (MQ=60)
                                                                                           ATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAA                                                          >  SRR19973065.627200/1‑150 (MQ=60)
                                                                                           ATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAA                                                          >  SRR19973065.627474/1‑150 (MQ=60)
                                                                                                  TTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAAC                                                   <  SRR19973065.2440344/150‑1 (MQ=60)
                                                                                                    ATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGT                                                 >  SRR19973065.2197408/1‑150 (MQ=60)
                                                                                                       GCAGGCGCAATCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGAAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTT                                              <  SRR19973065.1437582/150‑1 (MQ=60)
                                                                                                       GCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTT                                              <  SRR19973065.1437060/150‑1 (MQ=60)
                                                                                                                AACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCG                                     >  SRR19973065.1506356/1‑150 (MQ=60)
                                                                                                                AACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCG                                     >  SRR19973065.1506351/1‑150 (MQ=60)
                                                                                                                  CTCTATTTTCTTCGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGATAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAAAAACAGCAACGTCTTGAACAGGCGCT                                   <  SRR19973065.3339239/150‑1 (MQ=60)
                                                                                                                  CTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCT                                   <  SRR19973065.1591432/150‑1 (MQ=60)
                                                                                                                    CGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGA                                 >  SRR19973065.2518195/1‑150 (MQ=60)
                                                                                                                    CGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGA                                 >  SRR19973065.2518309/1‑150 (MQ=60)
                                                                                                                     GATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGAT                                >  SRR19973065.885131/1‑150 (MQ=60)
                                                                                                                     GATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGAT                                <  SRR19973065.2430245/150‑1 (MQ=60)
                                                                                                                           CTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCC                          >  SRR19973065.2804231/1‑150 (MQ=60)
                                                                                                                           CTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCC                          >  SRR19973065.2804276/1‑150 (MQ=60)
                                                                                                                            TACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCG                         >  SRR19973065.3271582/1‑150 (MQ=60)
                                                                                                                                 TTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACAC                    <  SRR19973065.333622/150‑1 (MQ=60)
                                                                                                                                 TTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACAC                    <  SRR19973065.333711/150‑1 (MQ=60)
                                                                                                                                     AAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGAC                >  SRR19973065.2490030/1‑150 (MQ=60)
                                                                                                                                     AAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGAC                >  SRR19973065.2490742/1‑150 (MQ=60)
                                                                                                                                      AACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACG               >  SRR19973065.762012/1‑150 (MQ=60)
                                                                                                                                      AACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACG               >  SRR19973065.762007/1‑150 (MQ=60)
                                                                                                                                        CTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAA             >  SRR19973065.2258172/1‑150 (MQ=60)
                                                                                                                                         TGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAAT            <  SRR19973065.457231/150‑1 (MQ=60)
                                                                                                                                         TGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAAT            >  SRR19973065.662996/1‑150 (MQ=60)
                                                                                                                                            TGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATT         <  SRR19973065.2058023/150‑1 (MQ=60)
                                                                                                                                            TGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATT         <  SRR19973065.202058/150‑1 (MQ=60)
                                                                                                                                            TGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATT         >  SRR19973065.1690061/1‑150 (MQ=60)
                                                                                                                                             GACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA        >  SRR19973065.2424776/1‑150 (MQ=60)
                                                                                                                                             GACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA        <  SRR19973065.3462423/150‑1 (MQ=60)
                                                                                                                                             GACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA        <  SRR19973065.2172071/150‑1 (MQ=60)
                                                                                                                                             GACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA        <  SRR19973065.3462414/150‑1 (MQ=60)
                                                                                                                                                CACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAA     >  SRR19973065.601864/1‑150 (MQ=60)
                                                                                                                                                CACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAA     >  SRR19973065.2371345/1‑150 (MQ=60)
                                                                                                                                                CACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAA     <  SRR19973065.3164506/150‑1 (MQ=60)
                                                                                                                                                 ACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAAC    >  SRR19973065.2855278/1‑150 (MQ=60)
                                                                                                                                                  CTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGTATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACG   <  SRR19973065.414999/150‑1 (MQ=60)
                                                                                                                                                  CTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACG   <  SRR19973065.415033/150‑1 (MQ=60)
                                                                                                                                                  CTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACG   <  SRR19973065.3336579/150‑1 (MQ=60)
                                                                                                                                                  CTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACG   <  SRR19973065.3336559/150‑1 (MQ=60)
                                                                                                                                                   TCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGC  >  SRR19973065.2864265/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTACAACGC  >  CP009273/805531‑805827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑