Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,257,232 G→T 100% R34S (CGC→AGC)  prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,257,2320GT97.4% 631.4 / 6.2 206R34S (CGC→AGC) prsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (110/90);  total (114/92)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 207 total aligned reads displayed.

GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCC  >  CP009273/1257087‑1257375
                                                                                                                                                 |                                                                                                                                               
gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAc                                                                                                                                               <  2:1553100/148‑1 (MQ=255)
gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAc                                                                                                                                               <  2:1553051/148‑1 (MQ=255)
   gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTa                                                                                                                                            <  1:1475044/148‑1 (MQ=255)
    aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGctacctac                                                                                                                                           <  2:239246/148‑1 (MQ=255)
     aGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACa                                                                                                                                          <  1:2108608/148‑1 (MQ=255)
     aGCACGACGCAGCGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACa                                                                                                                                          <  2:3202006/148‑1 (MQ=255)
      gCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAg                                                                                                                                         >  1:1171571/1‑148 (MQ=255)
      gCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAg                                                                                                                                         >  2:522597/1‑148 (MQ=255)
         cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcgg                                                                                                                                      >  1:2979471/1‑148 (MQ=255)
         cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcgg                                                                                                                                      >  1:2755254/1‑148 (MQ=255)
         cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAgcgg                                                                                                                                      >  1:265600/1‑148 (MQ=255)
           acgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcggcg                                                                                                                                    >  2:2026220/1‑148 (MQ=255)
              cAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCg                                                                                                                                 >  1:1597829/1‑148 (MQ=255)
                gggCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGcc                                                                                                                               >  2:1968371/1‑148 (MQ=255)
                   cATCAACCATAACGACTACTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAg                                                                                                                            <  2:2558419/148‑1 (MQ=255)
                   cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAg                                                                                                                            >  2:3228975/1‑148 (MQ=255)
                      cAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGa                                                                                                                         <  2:1295979/148‑1 (MQ=255)
                          cATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                     <  1:192608/148‑1 (MQ=255)
                          cATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                     >  2:2854850/1‑148 (MQ=255)
                               cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                >  1:19658/1‑148 (MQ=255)
                                 aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGc                                                                                                              >  2:473773/1‑148 (MQ=255)
                                  cTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                             <  2:182303/148‑1 (MQ=255)
                                   tAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                            <  1:208552/148‑1 (MQ=255)
                                   tAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                            >  1:3473680/1‑148 (MQ=255)
                                     aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGtt                                                                                                          <  2:1341944/148‑1 (MQ=255)
                                     aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTAATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGtt                                                                                                          >  2:39959/1‑148 (MQ=255)
                                       tCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTgg                                                                                                        <  2:3287023/148‑1 (MQ=255)
                                         cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCa                                                                                                      <  2:2787943/148‑1 (MQ=255)
                                         cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCa                                                                                                      <  2:332075/148‑1 (MQ=255)
                                            cAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATa                                                                                                   <  1:891623/148‑1 (MQ=255)
                                                     gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCt                                                                                          <  1:3358860/148‑1 (MQ=255)
                                                      ttAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTa                                                                                         <  1:489939/148‑1 (MQ=255)
                                                      ttAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTa                                                                                         <  1:1115246/148‑1 (MQ=255)
                                                       tagtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAg                                                                                        <  2:2966932/148‑1 (MQ=255)
                                                         gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGtt                                                                                      <  2:1771703/148‑1 (MQ=255)
                                                         gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGtt                                                                                      <  2:801844/148‑1 (MQ=255)
                                                          tagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTc                                                                                     <  1:1742422/148‑1 (MQ=255)
                                                            ggggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCg                                                                                   <  1:1913131/148‑1 (MQ=255)
                                                                 acaAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTg                                                                              >  2:2624236/1‑148 (MQ=255)
                                                                   aaGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGc                                                                            >  1:1458887/1‑148 (MQ=255)
                                                                    aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCACTACGTTGTGCTAGTTCCGGGGTGGCg                                                                           >  1:3370778/1‑148 (MQ=255)
                                                                    aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCg                                                                           >  1:1952522/1‑148 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTCCCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       >  2:698401/1‑148 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       <  1:257450/148‑1 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       <  2:2755254/148‑1 (MQ=255)
                                                                        gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAc                                                                       >  2:1038754/1‑148 (MQ=255)
                                                                           tGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAg                                                                    >  1:3481632/1‑148 (MQ=255)
                                                                                 atgaAAATATCACCACCGCGTACATTTTCAGTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaaaa                                                              >  1:2452360/1‑148 (MQ=255)
                                                                                  tgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAg                                                             >  2:737600/1‑148 (MQ=255)
                                                                                    aaaaTATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCt                                                           >  2:110602/1‑148 (MQ=255)
                                                                                      aaTATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTc                                                         <  1:1244622/148‑1 (MQ=255)
                                                                                       atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCa                                                        >  2:2808417/1‑148 (MQ=255)
                                                                                        tatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCat                                                       <  1:51685/148‑1 (MQ=255)
                                                                                        tatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCat                                                       >  2:2070185/1‑148 (MQ=255)
                                                                                         atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                                      >  2:3547225/1‑148 (MQ=255)
                                                                                          tCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCatat                                                     <  2:3501849/148‑1 (MQ=255)
                                                                                           caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                    >  2:175856/1‑148 (MQ=255)
                                                                                            accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCa                                                   >  2:1535705/1‑148 (MQ=255)
                                                                                            accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGAGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCa                                                   <  1:2232422/148‑1 (MQ=255)
                                                                                             ccaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                  >  1:235581/1‑148 (MQ=255)
                                                                                             ccaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                  >  1:1020094/1‑148 (MQ=255)
                                                                                             ccaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                  >  2:264292/1‑148 (MQ=255)
                                                                                              caccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAgg                                                 >  1:728666/1‑148 (MQ=255)
                                                                                                 cgcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAc                                              >  1:2601450/1‑148 (MQ=255)
                                                                                                 cgcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAc                                              <  1:262361/148‑1 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGTTTGGCAATACGTTGTGCTAGTTCAGGGGTGGCGATACCAGCAAAAAGCTTCATATCAGGCACgaga                                          >  2:823929/1‑148 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                          <  2:2758290/148‑1 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                          >  2:270717/1‑148 (MQ=255)
                                                                                                        aTTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaaga                                       <  1:2291950/148‑1 (MQ=255)
                                                                                                         ttttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAgaagaa                                      >  2:559872/1‑148 (MQ=255)
                                                                                                         ttttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAgaagaa                                      <  2:105891/148‑1 (MQ=255)
                                                                                                          tttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAc                                     >  2:3136416/1‑148 (MQ=255)
                                                                                                           ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAcc                                    >  1:684494/1‑148 (MQ=255)
                                                                                                           ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAcc                                    >  1:24700/1‑148 (MQ=255)
                                                                                                           ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAcc                                    >  1:684585/1‑148 (MQ=255)
                                                                                                                 aaTTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGc                              >  2:1347242/1‑148 (MQ=255)
                                                                                                                 aaTTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGc                              <  1:2854850/148‑1 (MQ=255)
                                                                                                                   tttGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCAt                            >  2:1821306/1‑148 (MQ=255)
                                                                                                                    ttGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATg                           >  1:2169482/1‑148 (MQ=255)
                                                                                                                       tACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGt                        >  1:3452804/1‑148 (MQ=255)
                                                                                                                       tACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGt                        >  1:3452259/1‑148 (MQ=255)
                                                                                                                         cGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTcc                      <  2:3342770/148‑1 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     <  1:1347229/148‑1 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     <  1:1347290/148‑1 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     >  1:2328946/1‑148 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                     <  1:892881/148‑1 (MQ=255)
                                                                                                                               cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                >  1:2437866/1‑148 (MQ=255)
                                                                                                                               cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                >  1:648477/1‑148 (MQ=255)
                                                                                                                               cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                >  2:2294522/1‑148 (MQ=255)
                                                                                                                                      aTCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGa         >  2:2847444/1‑148 (MQ=255)
                                                                                                                                      aTCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGa         <  1:926933/148‑1 (MQ=255)
                                                                                                                                       tCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGaa        <  1:3438998/148‑1 (MQ=255)
                                                                                                                                        cGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAt       <  1:3134090/148‑1 (MQ=255)
                                                                                                                                        cGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAt       >  2:3053638/1‑148 (MQ=255)
                                                                                                                                        cGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAt       >  2:2891961/1‑148 (MQ=255)
                                                                                                                                         gCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATTCGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATc      <  1:1866958/148‑1 (MQ=255)
                                                                                                                                         gCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATc      <  1:2765371/148‑1 (MQ=255)
                                                                                                                                           tAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTg    >  1:2460200/1‑148 (MQ=255)
                                                                                                                                            aaaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGc   <  1:2846257/148‑1 (MQ=255)
                                                                                                                                             aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGcc  >  2:1922690/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCC  >  CP009273/1257087‑1257375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG  >  CP009273/1257085‑1257376
                                                                                                                                                   |                                                                                                                                                
CTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAC                                                                                                                                                >  SRR19973066.3365023/1‑150 (MQ=60)
 TGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACC                                                                                                                                               <  SRR19973066.3224172/150‑1 (MQ=60)
     GAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACA                                                                                                                                           <  SRR19973066.1485432/150‑1 (MQ=60)
      AAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAG                                                                                                                                          >  SRR19973066.1179843/1‑150 (MQ=60)
       AGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGC                                                                                                                                         <  SRR19973066.2123159/150‑1 (MQ=60)
        GCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCG                                                                                                                                        <  SRR19973066.1154695/150‑1 (MQ=60)
         CACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGG                                                                                                                                       >  SRR19973066.2999150/1‑150 (MQ=60)
         CACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGG                                                                                                                                       >  SRR19973066.2773807/1‑150 (MQ=60)
         CACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGG                                                                                                                                       >  SRR19973066.2462089/1‑150 (MQ=60)
         CACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGG                                                                                                                                       >  SRR19973066.267588/1‑150 (MQ=60)
              CGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCG                                                                                                                                  >  SRR19973066.1609409/1‑150 (MQ=60)
                CAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTCAAGCTACCTACAGCGGCGTCGCC                                                                                                                                >  SRR19973066.942559/1‑150 (MQ=60)
                          ACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTG                                                                                                                      >  SRR19973066.74203/1‑150 (MQ=60)
                          ACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTG                                                                                                                      >  SRR19973066.1968259/1‑150 (MQ=60)
                           CCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGT                                                                                                                     <  SRR19973066.2961945/150‑1 (MQ=60)
                           CCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGT                                                                                                                     <  SRR19973066.1474352/150‑1 (MQ=60)
                           CCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGT                                                                                                                     <  SRR19973066.210390/150‑1 (MQ=60)
                            CATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTA                                                                                                                    <  SRR19973066.1111820/150‑1 (MQ=60)
                            CATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTA                                                                                                                    <  SRR19973066.194264/150‑1 (MQ=60)
                               AACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAG                                                                                                                 >  SRR19973066.3077530/1‑150 (MQ=60)
                               AACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAG                                                                                                                 >  SRR19973066.21948/1‑150 (MQ=60)
                               AACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAG                                                                                                                 >  SRR19973066.19952/1‑150 (MQ=60)
                                   ACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGG                                                                                                             >  SRR19973066.3496874/1‑150 (MQ=60)
                                   ACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGG                                                                                                             >  SRR19973066.2603350/1‑150 (MQ=60)
                                   ACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGG                                                                                                             >  SRR19973066.3496488/1‑150 (MQ=60)
                                     TAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTT                                                                                                           <  SRR19973066.210296/150‑1 (MQ=60)
                                              CAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACG                                                                                                  <  SRR19973066.2695320/150‑1 (MQ=60)
                                              CAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACG                                                                                                  <  SRR19973066.897938/150‑1 (MQ=60)
                                                 GTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTG                                                                                               >  SRR19973066.121461/1‑150 (MQ=60)
                                                       GTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAG                                                                                         >  SRR19973066.657134/1‑150 (MQ=60)
                                                       GTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAG                                                                                         <  SRR19973066.3381258/150‑1 (MQ=60)
                                                        TTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGT                                                                                        <  SRR19973066.1954897/150‑1 (MQ=60)
                                                        TTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGT                                                                                        <  SRR19973066.2300175/150‑1 (MQ=60)
                                                        TTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGT                                                                                        <  SRR19973066.493609/150‑1 (MQ=60)
                                                        TTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGT                                                                                        <  SRR19973066.1123297/150‑1 (MQ=60)
                                                          AGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTC                                                                                      <  SRR19973066.1929250/150‑1 (MQ=60)
                                                            TAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCG                                                                                    <  SRR19973066.1754977/150‑1 (MQ=60)
                                                              GGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGG                                                                                  <  SRR19973066.1926756/150‑1 (MQ=60)
                                                                   ACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGC                                                                             >  SRR19973066.1462544/1‑150 (MQ=60)
                                                                   ACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGC                                                                             >  SRR19973066.1469217/1‑150 (MQ=60)
                                                                    CAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCACTACGTTGTGCTAGTTCCGGGGTGGCG                                                                            >  SRR19973066.3393264/1‑150 (MQ=60)
                                                                    CAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCG                                                                            >  SRR19973066.1966342/1‑150 (MQ=60)
                                                                    CAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCG                                                                            <  SRR19973066.2544094/150‑1 (MQ=60)
                                                                      AGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTT                                                                          >  SRR19973066.2010930/1‑150 (MQ=60)
                                                                      AGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTT                                                                          >  SRR19973066.719369/1‑150 (MQ=60)
                                                                          GACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCA                                                                      <  SRR19973066.259397/150‑1 (MQ=60)
                                                                           ACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAG                                                                     >  SRR19973066.3504862/1‑150 (MQ=60)
                                                                           ACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAG                                                                     <  SRR19973066.3499526/150‑1 (MQ=60)
                                                                           ACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAG                                                                     <  SRR19973066.1724517/150‑1 (MQ=60)
                                                                               GATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAA                                                                 >  SRR19973066.357330/1‑150 (MQ=60)
                                                                               GATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAA                                                                 >  SRR19973066.1553275/1‑150 (MQ=60)
                                                                                 TGATGAAAATATCACCACCGCGTACATTTTCAGTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAA                                                               >  SRR19973066.2469073/1‑150 (MQ=60)
                                                                                    TGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCT                                                            >  SRR19973066.453463/1‑150 (MQ=60)
                                                                                       tggtagtgactggagttcagacgtgtgctcttccgatctTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCA                                                         <  SRR19973066.2323407/111‑1 (MQ=60)
                                                                                        AATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCAT                                                        <  SRR19973066.1253540/150‑1 (MQ=60)
                                                                                          TATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATAT                                                      >  SRR19973066.1298631/1‑150 (MQ=60)
                                                                                          TATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATAT                                                      <  SRR19973066.52345/150‑1 (MQ=60)
                                                                                           ATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATC                                                     <  SRR19973066.2793522/150‑1 (MQ=60)
                                                                                             CACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAG                                                   >  SRR19973066.237466/1‑150 (MQ=60)
                                                                                             CACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAG                                                   >  SRR19973066.1027599/1‑150 (MQ=60)
                                                                                             CACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAG                                                   >  SRR19973066.237436/1‑150 (MQ=60)
                                                                                              ACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGG                                                  >  SRR19973066.734069/1‑150 (MQ=60)
                                                                                              ACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGAGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGG                                                  <  SRR19973066.2247832/150‑1 (MQ=60)
                                                                                                CACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCA                                                <  SRR19973066.526433/150‑1 (MQ=60)
                                                                                                 ACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAC                                               >  SRR19973066.2619058/1‑150 (MQ=60)
                                                                                                 ACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAC                                               >  SRR19973066.2619052/1‑150 (MQ=60)
                                                                                                   CGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGA                                             <  SRR19973066.264330/150‑1 (MQ=60)
                                                                                                     CGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGA                                           <  SRR19973066.904286/150‑1 (MQ=60)
                                                                                                          ATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAC                                      <  SRR19973066.2307665/150‑1 (MQ=60)
                                                                                                           TTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACC                                     >  SRR19973066.689522/1‑150 (MQ=60)
                                                                                                           TTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACC                                     >  SRR19973066.25056/1‑150 (MQ=60)
                                                                                                           TTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACC                                     >  SRR19973066.689614/1‑150 (MQ=60)
                                                                                                           TTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACC                                     >  SRR19973066.689559/1‑150 (MQ=60)
                                                                                                                ATTTATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGG                                <  SRR19973066.2140848/150‑1 (MQ=60)
                                                                                                                   AATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCAT                             <  SRR19973066.2874031/150‑1 (MQ=60)
                                                                                                                    ATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATG                            >  SRR19973066.2184545/1‑150 (MQ=60)
                                                                                                                    ATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATG                            >  SRR19973066.2367910/1‑150 (MQ=60)
                                                                                                                       TGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGT                         >  SRR19973066.3475890/1‑150 (MQ=60)
                                                                                                                       TGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGT                         >  SRR19973066.508977/1‑150 (MQ=60)
                                                                                                                       TGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGT                         >  SRR19973066.3475257/1‑150 (MQ=60)
                                                                                                                       TGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGT                         >  SRR19973066.3475342/1‑150 (MQ=60)
                                                                                                                       TGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGT                         >  SRR19973066.3475349/1‑150 (MQ=60)
                                                                                                                         TACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCC                       <  SRR19973066.3392529/150‑1 (MQ=60)
                                                                                                                          ACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA                      >  SRR19973066.2344845/1‑150 (MQ=60)
                                                                                                                            GCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATT                    <  SRR19973066.899200/150‑1 (MQ=60)
                                                                                                                            GCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATT                    <  SRR19973066.1356998/150‑1 (MQ=60)
                                                                                                                            GCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATT                    <  SRR19973066.1357011/150‑1 (MQ=60)
                                                                                                                            GCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATT                    <  SRR19973066.1357061/150‑1 (MQ=60)
                                                                                                                              TGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGG                  >  SRR19973066.1796761/1‑150 (MQ=60)
                                                                                                                               GACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGT                 >  SRR19973066.653179/1‑150 (MQ=60)
                                                                                                                               GACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGT                 >  SRR19973066.2454472/1‑150 (MQ=60)
                                                                                                                                     GCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAG           <  SRR19973066.2040379/150‑1 (MQ=60)
                                                                                                                                      CCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGA          >  SRR19973066.898622/1‑150 (MQ=60)
                                                                                                                                        ATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAT        <  SRR19973066.266272/150‑1 (MQ=60)
                                                                                                                                        ATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAT        <  SRR19973066.266270/150‑1 (MQ=60)
                                                                                                                                        ATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAT        <  SRR19973066.933639/150‑1 (MQ=60)
                                                                                                                                         TCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATC       <  SRR19973066.3462002/150‑1 (MQ=60)
                                                                                                                                         TCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATC       <  SRR19973066.3461885/150‑1 (MQ=60)
                                                                                                                                          CGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCT      <  SRR19973066.3155008/150‑1 (MQ=60)
                                                                                                                                          CGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCT      <  SRR19973066.2208004/150‑1 (MQ=60)
                                                                                                                                           GCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATTCGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTG     <  SRR19973066.1880297/150‑1 (MQ=60)
                                                                                                                                           GCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTG     <  SRR19973066.2783994/150‑1 (MQ=60)
                                                                                                                                           GCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTG     >  SRR19973066.2477727/1‑150 (MQ=60)
                                                                                                                                           GCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTG     >  SRR19973066.2476967/1‑150 (MQ=60)
                                                                                                                                           GCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTG     >  SRR19973066.2476951/1‑150 (MQ=60)
                                                                                                                                           GCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTG     <  SRR19973066.1880306/150‑1 (MQ=60)
                                                                                                                                           GCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAGTGGTGGAAAGAATCTG     >  SRR19973066.2774445/1‑150 (MQ=60)
                                                                                                                                              AAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG  <  SRR19973066.2865393/150‑1 (MQ=60)
                                                                                                                                              AAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG  <  SRR19973066.2865382/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                
CTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG  >  CP009273/1257085‑1257376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑