Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,257,232 G→T 100% R34S (CGC→AGC)  prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,257,2320GT98.1% 853.4 / 4.8 269R34S (CGC→AGC) prsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (147/117);  total (148/121)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 271 total aligned reads displayed.

CTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAA  >  CP009273/1257085‑1257378
                                                                                                                                                   |                                                                                                                                                  
    ggAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGctacctac                                                                                                                                              <  1:4392973/150‑1 (MQ=255)
     gAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACa                                                                                                                                             <  1:4577474/150‑1 (MQ=255)
      aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAg                                                                                                                                            <  1:3309956/150‑1 (MQ=255)
       aGCACGACGCAGGGCATCAACCATAACGCCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgc                                                                                                                                           <  2:409243/150‑1 (MQ=255)
       aGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgc                                                                                                                                           <  1:376397/150‑1 (MQ=255)
        gCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcg                                                                                                                                          <  2:1526363/150‑1 (MQ=255)
         cACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcgg                                                                                                                                         <  2:2892687/150‑1 (MQ=255)
            gacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGt                                                                                                                                      >  2:2379939/1‑150 (MQ=255)
            gacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGt                                                                                                                                      >  2:3125010/1‑150 (MQ=255)
             acgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTc                                                                                                                                     <  1:92714/150‑1 (MQ=255)
              cgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCg                                                                                                                                    >  2:1723408/1‑150 (MQ=255)
              cgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCg                                                                                                                                    >  2:3328905/1‑150 (MQ=255)
                     cATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTg                                                                                                                             >  1:3530561/1‑150 (MQ=255)
                       tCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGaa                                                                                                                           >  2:5075545/1‑150 (MQ=255)
                          aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                        <  2:2081394/150‑1 (MQ=255)
                          aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtg                                                                                                                        >  2:2746673/1‑150 (MQ=255)
                           ccATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAgtgt                                                                                                                       <  2:5110285/150‑1 (MQ=255)
                               aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                   <  2:5033163/150‑1 (MQ=255)
                               aaCGACTAATTCCATCAGGTTGTCGGTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATATTCATTAATTTGTACGCTGACTAAGCCAACGCTAAAGATACATACACCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                   >  1:3652122/1‑150 (MQ=255)
                                  gACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                                >  2:4640176/1‑150 (MQ=255)
                                   aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                               >  2:3453619/1‑150 (MQ=255)
                                   aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTAAGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCgg                                                                                                               >  1:3573568/1‑150 (MQ=255)
                                     tAAGTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGtt                                                                                                             <  2:2963351/150‑1 (MQ=255)
                                       tttCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTgg                                                                                                           >  2:4527133/2‑150 (MQ=255)
                                       aTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTgg                                                                                                           >  1:4823903/1‑150 (MQ=255)
                                         tCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCa                                                                                                         <  1:4362972/150‑1 (MQ=255)
                                           cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                                       >  1:2764546/1‑150 (MQ=255)
                                            aTCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATa                                                                                                      <  1:5148168/150‑1 (MQ=255)
                                                ggTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGtt                                                                                                  <  1:1616315/150‑1 (MQ=255)
                                                  ttGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTtgt                                                                                                <  2:1783980/150‑1 (MQ=255)
                                                       gTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAg                                                                                           >  2:1248485/1‑150 (MQ=255)
                                                           gtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTcc                                                                                       <  1:1801684/150‑1 (MQ=255)
                                                            tagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCg                                                                                      >  1:572820/1‑150 (MQ=255)
                                                             agGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgg                                                                                     >  2:2338746/1‑150 (MQ=255)
                                                             agGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgg                                                                                     >  2:3723201/1‑150 (MQ=255)
                                                               gggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgggg                                                                                   <  2:2296551/150‑1 (MQ=255)
                                                               gggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCgggg                                                                                   <  1:3098645/150‑1 (MQ=255)
                                                                ggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGt                                                                                  <  1:2162342/150‑1 (MQ=255)
                                                                 gCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTg                                                                                 >  2:1993149/1‑150 (MQ=255)
                                                                 gCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTg                                                                                 >  2:6120/1‑150 (MQ=255)
                                                                   acaAGTGTACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGc                                                                               >  2:265329/1‑150 (MQ=255)
                                                                    caAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCg                                                                              >  1:3175967/1‑150 (MQ=255)
                                                                     aaGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGt                                                                             >  2:956265/1‑150 (MQ=255)
                                                                      aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGtt                                                                            <  1:4021721/150‑1 (MQ=255)
                                                                       gTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTa                                                                           >  2:1494097/1‑150 (MQ=255)
                                                                         ggACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAcc                                                                         >  2:1519468/1‑150 (MQ=255)
                                                                          gACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCa                                                                        <  2:4935399/150‑1 (MQ=255)
                                                                           aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAg                                                                       <  1:808139/150‑1 (MQ=255)
                                                                           aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAg                                                                       <  1:1929639/150‑1 (MQ=255)
                                                                            cTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGc                                                                      >  2:4020672/1‑150 (MQ=255)
                                                                            cTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGc                                                                      >  2:466459/1‑150 (MQ=255)
                                                                              ggATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaa                                                                    >  1:1078916/1‑150 (MQ=255)
                                                                               gatgatGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaa                                                                   >  2:3375894/1‑150 (MQ=255)
                                                                                  gatgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAg                                                                >  2:3292764/1‑150 (MQ=255)
                                                                                  gatgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAg                                                                >  2:710521/1‑150 (MQ=255)
                                                                                    tgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCt                                                              <  2:84133/150‑1 (MQ=255)
                                                                                         atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                                         >  1:4162857/1‑150 (MQ=255)
                                                                                         atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                                         <  2:1132729/150‑1 (MQ=255)
                                                                                           atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                       >  1:4917661/1‑150 (MQ=255)
                                                                                           atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                       >  1:1752242/1‑150 (MQ=255)
                                                                                           atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTACAGGGGTGGCGTTACCAGCAAAAAGCTTCATATc                                                       >  2:4148487/1‑150 (MQ=255)
                                                                                             caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                     >  1:1868406/1‑150 (MQ=255)
                                                                                             caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAg                                                     >  1:3692005/1‑150 (MQ=255)
                                                                                              accaccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAgg                                                    <  2:1664000/150‑1 (MQ=255)
                                                                                                 accGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAc                                                 >  1:5038469/1‑150 (MQ=255)
                                                                                                  ccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACg                                                <  1:541640/150‑1 (MQ=255)
                                                                                                    gcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgag                                              >  1:2375413/1‑150 (MQ=255)
                                                                                                     cgTACATTGTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                             <  1:2682589/150‑1 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            >  2:3888239/1‑150 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            <  1:1187901/150‑1 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            <  1:3590622/150‑1 (MQ=255)
                                                                                                      gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                            <  2:2163798/150‑1 (MQ=255)
                                                                                                       tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaag                                           <  2:5116885/150‑1 (MQ=255)
                                                                                                        aCATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaaga                                          <  1:131763/150‑1 (MQ=255)
                                                                                                              tCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCa                                    <  1:1396872/150‑1 (MQ=255)
                                                                                                              tCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCa                                    <  1:2078024/150‑1 (MQ=255)
                                                                                                                aTTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAgg                                  <  1:1324463/150‑1 (MQ=255)
                                                                                                                    aTTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAGGCTTCATATCAGGCACGAGAAGAACCTCAGGCATg                              >  2:757651/1‑150 (MQ=255)
                                                                                                                        gTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTc                          >  1:3610754/1‑150 (MQ=255)
                                                                                                                           cGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAt                       >  1:4106475/1‑150 (MQ=255)
                                                                                                                           cGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGGTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAt                       >  2:4618134/1‑150 (MQ=255)
                                                                                                                            gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAtt                      <  1:4280676/150‑1 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     >  2:1124441/1‑150 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     >  2:1263873/1‑150 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     <  2:2562983/150‑1 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     >  2:3538343/1‑150 (MQ=255)
                                                                                                                             cTGACTTCGCCATCGCTAAAGCTACCGACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                     <  2:4824037/150‑1 (MQ=255)
                                                                                                                              tGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGATCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtgg                    >  1:4619872/1‑150 (MQ=255)
                                                                                                                               gACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt                   <  1:2591265/150‑1 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtgg                 >  2:1824999/1‑150 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtgg                 >  1:2487325/1‑150 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtgg                 <  2:2366856/150‑1 (MQ=255)
                                                                                                                                       cATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGaa           >  1:471415/1‑150 (MQ=255)
                                                                                                                                       cATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGaa           >  2:4192532/1‑150 (MQ=255)
                                                                                                                                          cacTACAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTGACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCt        <  2:275822/148‑1 (MQ=255)
                                                                                                                                            cTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGc      <  2:1893619/150‑1 (MQ=255)
                                                                                                                                              aaaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCg    <  2:3651697/150‑1 (MQ=255)
                                                                                                                                               aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGa   >  2:4752730/1‑150 (MQ=255)
                                                                                                                                               aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGa   >  1:911988/1‑150 (MQ=255)
                                                                                                                                                aGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGaa  <  1:703620/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAA  >  CP009273/1257085‑1257378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAA  >  CP009273/1257084‑1257378
                                                                                                                                                    |                                                                                                                                                  
CCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTA                                                                                                                                                   >  SRR19973063.2585234/1‑150 (MQ=60)
   GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCT                                                                                                                                                <  SRR19973063.3596968/150‑1 (MQ=60)
   GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCT                                                                                                                                                <  SRR19973063.3597990/150‑1 (MQ=60)
   GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCT                                                                                                                                                <  SRR19973063.3849395/150‑1 (MQ=60)
     GGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTAC                                                                                                                                              <  SRR19973063.4407039/150‑1 (MQ=60)
      GAAGCACGACGCAGTGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACA                                                                                                                                             <  SRR19973063.4592131/150‑1 (MQ=60)
      GAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACA                                                                                                                                             <  SRR19973063.4592115/150‑1 (MQ=60)
       AAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAG                                                                                                                                            <  SRR19973063.3320543/150‑1 (MQ=60)
        AGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGC                                                                                                                                           <  SRR19973063.377438/150‑1 (MQ=60)
        AGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGC                                                                                                                                           <  SRR19973063.377471/150‑1 (MQ=60)
          CACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGG                                                                                                                                         >  SRR19973063.3663407/1‑150 (MQ=60)
           ACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGC                                                                                                                                        <  SRR19973063.5149920/150‑1 (MQ=60)
            CGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGTACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCTCTAAAGCTACCTACAGCGGCG                                                                                                                                       >  SRR19973063.4361951/1‑150 (MQ=60)
            CGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCG                                                                                                                                       >  SRR19973063.4361870/1‑150 (MQ=60)
             GACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGT                                                                                                                                      <  SRR19973063.1636760/150‑1 (MQ=60)
             GACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGT                                                                                                                                      >  SRR19973063.2603532/1‑150 (MQ=60)
              ACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTC                                                                                                                                     <  SRR19973063.93029/150‑1 (MQ=60)
                    GGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAG                                                                                                                               >  SRR19973063.2922645/1‑150 (MQ=60)
                     GCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGT                                                                                                                              <  SRR19973063.876732/150‑1 (MQ=60)
                      CATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTG                                                                                                                             >  SRR19973063.3541871/1‑150 (MQ=60)
                         CAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAG                                                                                                                          >  SRR19973063.3534889/1‑150 (MQ=60)
                             CATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTA                                                                                                                      <  SRR19973063.1778228/150‑1 (MQ=60)
                                AACGACTAATTCCATCAGGTTGTCGGTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATATTCATTAATTTGTACGCTGACTAAGCCAACGCTAAAGATACATACACCGGCGTCGCCGAGTGAAGTGTACAG                                                                                                                   >  SRR19973063.3663833/1‑150 (MQ=60)
                                  CGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGC                                                                                                                 >  SRR19973063.4188642/1‑150 (MQ=60)
                                   GACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCG                                                                                                                <  SRR19973063.3213525/150‑1 (MQ=60)
                                    ACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTAAGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGG                                                                                                               >  SRR19973063.3585027/1‑150 (MQ=60)
                                       AATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTG                                                                                                            >  SRR19973063.5166402/1‑150 (MQ=60)
                                       AATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATAAGAAAGCTGACTTCGCCATCGATAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTG                                                                                                            >  SRR19973063.1356623/1‑150 (MQ=60)
                                        ATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGG                                                                                                           >  SRR19973063.4839349/1‑150 (MQ=60)
                                        ATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGG                                                                                                           >  SRR19973063.1228743/1‑150 (MQ=60)
                                          TCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCA                                                                                                         <  SRR19973063.4104788/150‑1 (MQ=60)
                                          TCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCA                                                                                                         <  SRR19973063.4376925/150‑1 (MQ=60)
                                            CATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAT                                                                                                       >  SRR19973063.2773459/1‑150 (MQ=60)
                                            CATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAT                                                                                                       >  SRR19973063.2773227/1‑150 (MQ=60)
                                             ATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATA                                                                                                      >  SRR19973063.4545588/1‑150 (MQ=60)
                                             ATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATA                                                                                                      <  SRR19973063.5164739/150‑1 (MQ=60)
                                             ATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATA                                                                                                      <  SRR19973063.5164865/150‑1 (MQ=60)
                                               CAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACG                                                                                                    <  SRR19973063.267492/150‑1 (MQ=60)
                                               CAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACG                                                                                                    >  SRR19973063.777650/1‑150 (MQ=60)
                                                AGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGT                                                                                                   >  SRR19973063.4839680/1‑150 (MQ=60)
                                                AGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGT                                                                                                   >  SRR19973063.4839690/1‑150 (MQ=60)
                                                AGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATAACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGT                                                                                                   >  SRR19973063.941661/1‑150 (MQ=60)
                                                 GGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTT                                                                                                  <  SRR19973063.1621259/150‑1 (MQ=60)
                                                  GTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACACTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTG                                                                                                 >  SRR19973063.688169/1‑150 (MQ=60)
                                                    TGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTG                                                                                               >  SRR19973063.950928/1‑150 (MQ=60)
                                                     GTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGC                                                                                              <  SRR19973063.4951433/150‑1 (MQ=60)
                                                            GTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC                                                                                       <  SRR19973063.1807267/150‑1 (MQ=60)
                                                             TAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCG                                                                                      <  SRR19973063.1535677/150‑1 (MQ=60)
                                                             TAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCG                                                                                      >  SRR19973063.574511/1‑150 (MQ=60)
                                                                GGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGG                                                                                   <  SRR19973063.3108617/150‑1 (MQ=60)
                                                                 GGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGT                                                                                  <  SRR19973063.2169113/150‑1 (MQ=60)
                                                                    ACAAGTGGANTGGATGATGAAAATATCNCNACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGC                                                                               >  SRR19973063.3728290/1‑150 (MQ=60)
                                                                     CAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCG                                                                              >  SRR19973063.3186185/1‑150 (MQ=60)
                                                                       AGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTT                                                                            <  SRR19973063.4034623/150‑1 (MQ=60)
                                                                            ACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAG                                                                       <  SRR19973063.1935671/150‑1 (MQ=60)
                                                                            ACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAG                                                                       <  SRR19973063.1845478/150‑1 (MQ=60)
                                                                            ACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAG                                                                       <  SRR19973063.810469/150‑1 (MQ=60)
                                                                               GGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAA                                                                    >  SRR19973063.1082237/1‑150 (MQ=60)
                                                                               GGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAA                                                                    >  SRR19973063.1082199/1‑150 (MQ=60)
                                                                                          ATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATC                                                         >  SRR19973063.4176169/1‑150 (MQ=60)
                                                                                          ATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATA                                                         >  SRR19973063.1899543/1‑150 (MQ=60)
                                                                                          ATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATA                                                         >  SRR19973063.4176167/1‑150 (MQ=60)
                                                                                            ATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATC                                                       >  SRR19973063.1757620/1‑150 (MQ=60)
                                                                                            ATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATC                                                       >  SRR19973063.4933539/1‑150 (MQ=60)
                                                                                             TCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCA                                                      <  SRR19973063.1946343/150‑1 (MQ=60)
                                                                                              CACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGTCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAG                                                     >  SRR19973063.3703783/1‑150 (MQ=60)
                                                                                              CACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAG                                                     >  SRR19973063.1874243/1‑150 (MQ=60)
                                                                                              CACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAG                                                     >  SRR19973063.1874253/1‑150 (MQ=60)
                                                                                              CACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAG                                                     >  SRR19973063.3703826/1‑150 (MQ=60)
                                                                                                 CACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCA                                                  <  SRR19973063.201351/150‑1 (MQ=60)
                                                                                                 CACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCA                                                  <  SRR19973063.2387565/150‑1 (MQ=60)
                                                                                                  ACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCAC                                                 >  SRR19973063.5054707/1‑150 (MQ=60)
                                                                                                   CCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACG                                                <  SRR19973063.543242/150‑1 (MQ=60)
                                                                                                   CCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACG                                                <  SRR19973063.543290/150‑1 (MQ=60)
                                                                                                   CCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACG                                                <  SRR19973063.543475/150‑1 (MQ=60)
                                                                                                   CCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACG                                                >  SRR19973063.5161825/1‑150 (MQ=60)
                                                                                                     GCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAG                                              >  SRR19973063.2383023/1‑150 (MQ=60)
                                                                                                     GCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAG                                              >  SRR19973063.2383026/1‑150 (MQ=60)
                                                                                                      CGTACATTGTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGA                                             <  SRR19973063.2691233/150‑1 (MQ=60)
                                                                                                       GTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAA                                            <  SRR19973063.1728663/150‑1 (MQ=60)
                                                                                                       GTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAA                                            <  SRR19973063.3602137/150‑1 (MQ=60)
                                                                                                       GTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAA                                            <  SRR19973063.1191473/150‑1 (MQ=60)
                                                                                                       GTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAA                                            <  SRR19973063.2356419/150‑1 (MQ=60)
                                                                                                         ACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGA                                          <  SRR19973063.132183/150‑1 (MQ=60)
                                                                                                             TTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTATCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCT                                      <  SRR19973063.2848414/150‑1 (MQ=60)
                                                                                                             TTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCT                                      <  SRR19973063.1305958/150‑1 (MQ=60)
                                                                                                               TCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCA                                    <  SRR19973063.1401099/150‑1 (MQ=60)
                                                                                                               TCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCA                                    <  SRR19973063.2084519/150‑1 (MQ=60)
                                                                                                                 ATTAATTTGTACTCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGG                                  <  SRR19973063.735233/150‑1 (MQ=60)
                                                                                                                 ATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGG                                  <  SRR19973063.1328425/150‑1 (MQ=60)
                                                                                                                 ATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGG                                  <  SRR19973063.1328385/150‑1 (MQ=60)
                                                                                                                      TTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGC                             >  SRR19973063.4000536/1‑150 (MQ=60)
                                                                                                                       TTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCG                            >  SRR19973063.4126116/1‑150 (MQ=60)
                                                                                                                        TGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGT                           >  SRR19973063.4398848/1‑150 (MQ=60)
                                                                                                                        TGTACGCTGACTTCGCCATAGCTAAAGCTACCTACAGCGTCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTATTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGT                           >  SRR19973063.1597204/1‑150 (MQ=60)
                                                                                                                         GTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTC                          >  SRR19973063.3622348/1‑150 (MQ=60)
                                                                                                                          TACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCC                         >  SRR19973063.3289447/1‑150 (MQ=60)
                                                                                                                           ACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCA                        >  SRR19973063.4484000/1‑150 (MQ=60)
                                                                                                                            CGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCAT                       >  SRR19973063.4119626/1‑150 (MQ=60)
                                                                                                                             GCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATT                      <  SRR19973063.4294277/150‑1 (MQ=60)
                                                                                                                              CTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTG                     <  SRR19973063.1871358/150‑1 (MQ=60)
                                                                                                                               TGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGG                    >  SRR19973063.4634434/1‑150 (MQ=60)
                                                                                                                               TGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGG                    >  SRR19973063.3610020/1‑150 (MQ=60)
                                                                                                                               TGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGATCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGG                    >  SRR19973063.4634633/1‑150 (MQ=60)
                                                                                                                                GACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGT                   <  SRR19973063.2599502/150‑1 (MQ=60)
                                                                                                                                 ACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTG                  <  SRR19973063.3646292/150‑1 (MQ=60)
                                                                                                                                  CTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCCTTGGTGG                 >  SRR19973063.1327949/1‑150 (MQ=60)
                                                                                                                                  CTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGG                 >  SRR19973063.2495272/1‑150 (MQ=60)
                                                                                                                                  CTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGG                 <  SRR19973063.4818076/150‑1 (MQ=60)
                                                                                                                                  CTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGG                 <  SRR19973063.4818059/150‑1 (MQ=60)
                                                                                                                                   TTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGA                >  SRR19973063.2714166/1‑150 (MQ=60)
                                                                                                                                   TTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGA                >  SRR19973063.827588/1‑150 (MQ=60)
                                                                                                                                    TCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAA               <  SRR19973063.3611999/150‑1 (MQ=60)
                                                                                                                                    TCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAA               >  SRR19973063.4135376/1‑150 (MQ=60)
                                                                                                                                      GCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAG             <  SRR19973063.3549680/150‑1 (MQ=60)
                                                                                                                                        CATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAA           >  SRR19973063.472744/1‑150 (MQ=60)
                                                                                                                                        CATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAA           >  SRR19973063.472718/1‑150 (MQ=60)
                                                                                                                                         ATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAT          >  SRR19973063.2160138/1‑150 (MQ=60)
                                                                                                                                          TCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATC         <  SRR19973063.3400536/150‑1 (MQ=60)
                                                                                                                                             CTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGC      <  SRR19973063.3135073/150‑1 (MQ=60)
                                                                                                                                               AAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG    <  SRR19973063.2588439/150‑1 (MQ=60)
                                                                                                                                                AAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGA   >  SRR19973063.914670/1‑150 (MQ=60)
                                                                                                                                                AAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGA   >  SRR19973063.915216/1‑150 (MQ=60)
                                                                                                                                                AAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGA   >  SRR19973063.2271799/1‑150 (MQ=60)
                                                                                                                                                 AGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAA  <  SRR19973063.705688/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCGAA  >  CP009273/1257084‑1257378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑