Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,514,858 G→A 100% E114K (GAA→AAA)  mcbR → colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,514,8580GA99.6% 791.5 / ‑4.6 237E114K (GAA→AAA) mcbRcolanic acid and biofilm gene transcriptional regulator, MqsR‑controlled
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (100/136);  total (101/136)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

TGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA  >  CP009273/1514713‑1515002
                                                                                                                                                 |                                                                                                                                                
tGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGaaa                                                                                                                                                <  1:2810920/148‑1 (MQ=255)
 gTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAAt                                                                                                                                               <  1:2156049/148‑1 (MQ=255)
  tCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGaaagt                                                                                                                                              >  2:2853846/1‑146 (MQ=255)
   cGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTg                                                                                                                                             >  2:1717879/1‑148 (MQ=255)
   cGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTg                                                                                                                                             <  1:360957/148‑1 (MQ=255)
        cTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCga                                                                                                                                        >  2:844085/1‑148 (MQ=255)
             caAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAAt                                                                                                                                   <  2:1262009/148‑1 (MQ=255)
             caAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAAt                                                                                                                                   <  2:3544029/148‑1 (MQ=255)
               aGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTa                                                                                                                                 <  2:3343527/148‑1 (MQ=255)
               aGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTa                                                                                                                                 <  2:2378864/148‑1 (MQ=255)
               aGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTa                                                                                                                                 <  2:2494127/148‑1 (MQ=255)
                gCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAc                                                                                                                                >  2:1902586/1‑148 (MQ=255)
                gCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAc                                                                                                                                >  2:804231/1‑148 (MQ=255)
                        aGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGgcg                                                                                                                        >  2:3561320/1‑148 (MQ=255)
                          ttCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                                      >  2:1232090/1‑148 (MQ=255)
                           tCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCaa                                                                                                                     >  1:1993233/1‑148 (MQ=255)
                                aaGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGACTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAACTTGCTCGAGAAAGTACAGCAGGCGCAAGaaaa                                                                                                                >  2:2273981/1‑148 (MQ=255)
                                aaGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa                                                                                                                <  2:1341844/148‑1 (MQ=255)
                                aaGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa                                                                                                                <  2:944159/148‑1 (MQ=255)
                                 aGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAg                                                                                                               <  2:1396397/148‑1 (MQ=255)
                                   tGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAggg                                                                                                             >  1:3316885/1‑148 (MQ=255)
                                     gggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCg                                                                                                           <  2:2076815/148‑1 (MQ=255)
                                      ggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTAGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGa                                                                                                          <  1:2030709/148‑1 (MQ=255)
                                       gAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAc                                                                                                         >  1:3091183/1‑148 (MQ=255)
                                        aaaCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACa                                                                                                        <  2:1590905/148‑1 (MQ=255)
                                          aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg                                                                                                      >  1:556303/1‑148 (MQ=255)
                                            gTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGa                                                                                                    <  1:1254958/148‑1 (MQ=255)
                                            gTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGa                                                                                                    <  1:844085/148‑1 (MQ=255)
                                                aTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaa                                                                                                >  1:3226824/1‑148 (MQ=255)
                                                 ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                               >  1:3356839/1‑148 (MQ=255)
                                                 ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                               <  1:1406752/148‑1 (MQ=255)
                                                 ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                               <  1:2690698/148‑1 (MQ=255)
                                                 ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                               >  1:2537170/1‑148 (MQ=255)
                                                    gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                                            <  1:3059119/148‑1 (MQ=255)
                                                    gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                                            <  1:2458185/148‑1 (MQ=255)
                                                    gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                                            <  1:1148034/148‑1 (MQ=255)
                                                     aCGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAt                                                                                           <  2:2123930/148‑1 (MQ=255)
                                                        aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                                        <  2:2030878/148‑1 (MQ=255)
                                                           tcaatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGACCAAATCATTAATGt                                                                                     >  2:700182/1‑148 (MQ=255)
                                                              atcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTaaa                                                                                  >  1:980505/1‑148 (MQ=255)
                                                               tcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAc                                                                                 >  1:3408321/1‑148 (MQ=255)
                                                               tcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAc                                                                                 >  2:2899776/1‑148 (MQ=255)
                                                               tcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAc                                                                                 >  1:521130/1‑148 (MQ=255)
                                                                  gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                              <  2:1769023/148‑1 (MQ=255)
                                                                  gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                              <  2:3117366/148‑1 (MQ=255)
                                                                   aTCCGCTACGAACTGGAATTAATGGCAGTAGCTCTGGCTGTTTAAAACCTCACCCCGCAAGACCTTGAGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACATGc                                                                             >  1:229765/1‑148 (MQ=255)
                                                                    tCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCt                                                                            >  2:3020854/1‑148 (MQ=255)
                                                                    tCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACAGGGAACAAATCATTAATGTAAACAGGCt                                                                            >  2:1507441/1‑148 (MQ=255)
                                                                     ccGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACa                                                                                >  2:3081515/1‑143 (MQ=255)
                                                                     ccGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTa                                                                           <  1:2870855/148‑1 (MQ=255)
                                                                      cGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAt                                                                          <  2:1691751/148‑1 (MQ=255)
                                                                        cTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAttt                                                                        >  2:451895/1‑148 (MQ=255)
                                                                          aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg                                                                      >  2:103071/1‑148 (MQ=255)
                                                                           cGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGc                                                                     >  1:596751/1‑148 (MQ=255)
                                                                            gAACTGGAATTAATGGCAGTTGCTCTGTCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt                                                                    <  1:2065709/148‑1 (MQ=255)
                                                                            gAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt                                                                    <  1:3031481/148‑1 (MQ=255)
                                                                             aaCTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCtt                                                                   <  1:1088887/148‑1 (MQ=255)
                                                                               cTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAg                                                                 >  1:1670775/1‑148 (MQ=255)
                                                                                  gAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGa                                                              <  2:842503/148‑1 (MQ=255)
                                                                                   aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                                             <  2:2480275/148‑1 (MQ=255)
                                                                                      tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa                                                          >  2:1965637/1‑148 (MQ=255)
                                                                                       aaTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTat                                                         <  2:1632938/148‑1 (MQ=255)
                                                                                        aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        >  1:1370727/1‑148 (MQ=255)
                                                                                        aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        <  1:1777691/148‑1 (MQ=255)
                                                                                        aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        >  1:2974165/1‑148 (MQ=255)
                                                                                        aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        <  2:193547/148‑1 (MQ=255)
                                                                                         tGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatca                                                       >  2:837217/1‑148 (MQ=255)
                                                                                          ggCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcat                                                      >  1:2640063/1‑148 (MQ=255)
                                                                                          ggCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcat                                                      >  1:1050989/1‑148 (MQ=255)
                                                                                            cAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCg                                                    <  2:1654573/148‑1 (MQ=255)
                                                                                               ttGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                                 >  2:1856189/1‑148 (MQ=255)
                                                                                                 gCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCaa                                               <  1:833200/148‑1 (MQ=255)
                                                                                                 gCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCaa                                               <  1:676029/148‑1 (MQ=255)
                                                                                                 gCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCaa                                               <  1:3480260/148‑1 (MQ=255)
                                                                                                  ctctGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCaaa                                              <  1:89862/148‑1 (MQ=255)
                                                                                                      ggCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATg                                          >  2:2413004/1‑148 (MQ=255)
                                                                                                       gCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGc                                         >  1:3170266/1‑148 (MQ=255)
                                                                                                         tGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGccc                                       <  1:286402/148‑1 (MQ=255)
                                                                                                          gTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa                                      >  2:2825107/1‑148 (MQ=255)
                                                                                                           ttGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCAt                                     <  1:2589570/148‑1 (MQ=255)
                                                                                                            tGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATc                                    <  1:1294835/148‑1 (MQ=255)
                                                                                                                aaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgt                                <  1:3061950/148‑1 (MQ=255)
                                                                                                                aaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgt                                >  2:1792200/1‑148 (MQ=255)
                                                                                                                 aCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtg                               <  1:247867/148‑1 (MQ=255)
                                                                                                                  ccTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgt                              >  2:1679790/1‑148 (MQ=255)
                                                                                                                  ccTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgt                              <  1:707577/148‑1 (MQ=255)
                                                                                                                     cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag                           <  2:3226824/148‑1 (MQ=255)
                                                                                                                     cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag                           <  2:3400675/148‑1 (MQ=255)
                                                                                                                      aCCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga                          <  2:73505/148‑1 (MQ=255)
                                                                                                                         tcGCAAGACCTTGCTGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatga                       <  2:2889480/147‑1 (MQ=255)
                                                                                                                         ccGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatga                       >  1:1956012/1‑148 (MQ=255)
                                                                                                                          cGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatgat                      >  1:2223822/1‑148 (MQ=255)
                                                                                                                          cGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatgat                      >  1:1110785/1‑148 (MQ=255)
                                                                                                                              aGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAg                  <  2:2766383/148‑1 (MQ=255)
                                                                                                                               gACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGc                 >  2:2668957/1‑148 (MQ=255)
                                                                                                                                aCCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCa                <  2:145601/148‑1 (MQ=255)
                                                                                                                                aCCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCa                <  2:3229728/148‑1 (MQ=255)
                                                                                                                                    tGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtg            <  1:1286873/148‑1 (MQ=255)
                                                                                                                                    tGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtg            <  1:2756056/148‑1 (MQ=255)
                                                                                                                                              cAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGa  <  1:1143610/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA  >  CP009273/1514713‑1515002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGG  >  CP009273/1514709‑1515005
                                                                                                                                                     |                                                                                                                                                   
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGA                                                                                                                                                     <  SRR19973066.3586467/150‑1 (MQ=60)
    TGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATT                                                                                                                                                 <  SRR19973066.2829829/150‑1 (MQ=60)
     GTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTG                                                                                                                                                <  SRR19973066.2171022/150‑1 (MQ=60)
       CGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCT                                                                                                                                              <  SRR19973066.363776/150‑1 (MQ=60)
         CACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCG                                                                                                                                            >  SRR19973066.1351643/1‑150 (MQ=60)
         CACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCG                                                                                                                                            >  SRR19973066.1351584/1‑150 (MQ=60)
              GCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAA                                                                                                                                       <  SRR19973066.2035475/150‑1 (MQ=60)
                ACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATT                                                                                                                                     >  SRR19973066.198343/1‑150 (MQ=60)
                ACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATT                                                                                                                                     >  SRR19973066.198359/1‑150 (MQ=60)
                             GTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAA                                                                                                                        >  SRR19973066.2007258/1‑150 (MQ=60)
                                 CGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAA                                                                                                                    >  SRR19973066.2784412/1‑150 (MQ=60)
                                     AGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGG                                                                                                                >  SRR19973066.3130974/1‑150 (MQ=60)
                                     AGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGG                                                                                                                >  SRR19973066.3338906/1‑150 (MQ=60)
                                       TGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCG                                                                                                              <  SRR19973066.673170/150‑1 (MQ=60)
                                         GGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAC                                                                                                            >  SRR19973066.2153220/1‑150 (MQ=60)
                                         GGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAC                                                                                                            >  SRR19973066.1272230/1‑150 (MQ=60)
                                         GGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAC                                                                                                            >  SRR19973066.1272202/1‑150 (MQ=60)
                                         GGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAC                                                                                                            >  SRR19973066.3111834/1‑150 (MQ=60)
                                          GGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTAGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACA                                                                                                           <  SRR19973066.2044890/150‑1 (MQ=60)
                                            AAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATG                                                                                                         >  SRR19973066.560291/1‑150 (MQ=60)
                                                GTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGACC                                                                                                     >  SRR19973066.2655625/1‑150 (MQ=60)
                                                GTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAAC                                                                                                     <  SRR19973066.3339258/150‑1 (MQ=60)
                                                GTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAAC                                                                                                     <  SRR19973066.1263994/150‑1 (MQ=60)
                                                GTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAAC                                                                                                     <  SRR19973066.850111/150‑1 (MQ=60)
                                                GTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAAC                                                                                                     <  SRR19973066.850214/150‑1 (MQ=60)
                                                  CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAA                                                                                                   >  SRR19973066.3423379/1‑150 (MQ=60)
                                                  CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAA                                                                                                   >  SRR19973066.3248153/1‑150 (MQ=60)
                                                   AATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAA                                                                                                  >  SRR19973066.2554416/1‑150 (MQ=60)
                                                   AATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAA                                                                                                  >  SRR19973066.3379210/1‑150 (MQ=60)
                                                    ATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAAT                                                                                                 <  SRR19973066.1713197/150‑1 (MQ=60)
                                                     TTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATC                                                                                                <  SRR19973066.146739/150‑1 (MQ=60)
                                                     TTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATC                                                                                                <  SRR19973066.1416867/150‑1 (MQ=60)
                                                     TTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATC                                                                                                <  SRR19973066.2708661/150‑1 (MQ=60)
                                                      TAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCGAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCA                                                                                               <  SRR19973066.2392654/150‑1 (MQ=60)
                                                       AGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAT                                                                                              >  SRR19973066.2785011/1‑150 (MQ=60)
                                                        GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                             <  SRR19973066.2474941/150‑1 (MQ=60)
                                                        GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                             <  SRR19973066.3079580/150‑1 (MQ=60)
                                                        GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                             <  SRR19973066.1156224/150‑1 (MQ=60)
                                                        GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                             <  SRR19973066.3080752/150‑1 (MQ=60)
                                                           GAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAT                                                                                          <  SRR19973066.555403/150‑1 (MQ=60)
                                                           GAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAT                                                                                          <  SRR19973066.888864/150‑1 (MQ=60)
                                                           GAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAT                                                                                          <  SRR19973066.555480/150‑1 (MQ=60)
                                                           GAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAT                                                                                          <  SRR19973066.3013649/150‑1 (MQ=60)
                                                           GAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAT                                                                                          <  SRR19973066.360917/150‑1 (MQ=60)
                                                                CAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAA                                                                                     >  SRR19973066.2741122/1‑150 (MQ=60)
                                                                CAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAA                                                                                     >  SRR19973066.987737/1‑150 (MQ=60)
                                                                CAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAA                                                                                     >  SRR19973066.987762/1‑150 (MQ=60)
                                                                 AATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAC                                                                                    >  SRR19973066.3431097/1‑150 (MQ=60)
                                                                 AATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAC                                                                                    >  SRR19973066.524964/1‑150 (MQ=60)
                                                                 AATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAC                                                                                    <  SRR19973066.847090/150‑1 (MQ=60)
                                                                    tctATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGG                                                                                 <  SRR19973066.3102099/147‑1 (MQ=60)
                                                                    CGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGG                                                                                 >  SRR19973066.2841345/1‑150 (MQ=60)
                                                                     GGATCCGCTACGAACTGGAATTAATGGCAGTAGCTCTGGCTGTTTAAAACCTCACCCCGCAAGACCTTGAGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACATGC                                                                                >  SRR19973066.231596/1‑150 (MQ=60)
                                                                      GATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCT                                                                               <  SRR19973066.1867133/150‑1 (MQ=60)
                                                                       ATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTA                                                                              <  SRR19973066.640365/150‑1 (MQ=60)
                                                                        TCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAT                                                                             <  SRR19973066.2668994/150‑1 (MQ=60)
                                                                         CCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATT                                                                            <  SRR19973066.2890109/150‑1 (MQ=60)
                                                                         CCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATT                                                                            <  SRR19973066.2889990/150‑1 (MQ=60)
                                                                             TACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGC                                                                        >  SRR19973066.2276469/1‑150 (MQ=60)
                                                                             TACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGC                                                                        >  SRR19973066.600877/1‑150 (MQ=60)
                                                                                GAACTGGAATTAATGGCAGTTGCTCTGTCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTA                                                                     <  SRR19973066.2080016/150‑1 (MQ=60)
                                                                                GAACTGGAATTAATGGCAGTTGCTCTGGCTGTTTAAAACCTCACCCCGCAAGGCCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTA                                                                     <  SRR19973066.3052259/150‑1 (MQ=60)
                                                                                GAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTA                                                                     <  SRR19973066.266388/150‑1 (MQ=60)
                                                                                GAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTA                                                                     <  SRR19973066.1137898/150‑1 (MQ=60)
                                                                                GAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTA                                                                     <  SRR19973066.3051734/150‑1 (MQ=60)
                                                                                 AACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAG                                                                    >  SRR19973066.1682947/1‑150 (MQ=60)
                                                                                 AACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAG                                                                    <  SRR19973066.1096828/150‑1 (MQ=60)
                                                                                   CTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCG                                                                  >  SRR19973066.1074173/1‑150 (MQ=60)
                                                                                     GGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAT                                                                <  SRR19973066.2499364/150‑1 (MQ=60)
                                                                                          TAATGGCAGTTTCTCTGGCTGTTGAAAACCTGTCCGCGCTAGATCTTGCGGAACTCCAGACATGGCTCGAGAACTTACAGCAGTCGCATGAAAAGGGCGACATGGAACAAATCATTAATGTAACCAGGCTATTTCGCTTAGCGATTTATC                                                           <  SRR19973066.628615/150‑1 (MQ=60)
                                                                                          TAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATC                                                           >  SRR19973066.1380943/1‑150 (MQ=60)
                                                                                          TAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATC                                                           >  SRR19973066.2993823/1‑150 (MQ=60)
                                                                                          TAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATC                                                           >  SRR19973066.1380645/1‑150 (MQ=60)
                                                                                          TAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATC                                                           >  SRR19973066.2074923/1‑150 (MQ=60)
                                                                                            ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                         <  SRR19973066.2162838/150‑1 (MQ=60)
                                                                                            ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                         >  SRR19973066.2657825/1‑150 (MQ=60)
                                                                                            ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                         <  SRR19973066.1790401/150‑1 (MQ=60)
                                                                                            ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                         >  SRR19973066.1058716/1‑150 (MQ=60)
                                                                                             TGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATC                                                        <  SRR19973066.3227899/150‑1 (MQ=60)
                                                                                                     GCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAAT                                                <  SRR19973066.3503484/150‑1 (MQ=60)
                                                                                                     GCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAAT                                                <  SRR19973066.839274/150‑1 (MQ=60)
                                                                                                     GCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAAT                                                <  SRR19973066.680976/150‑1 (MQ=60)
                                                                                                      CTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATA                                               <  SRR19973066.90978/150‑1 (MQ=60)
                                                                                                      CTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATA                                               <  SRR19973066.90838/150‑1 (MQ=60)
                                                                                                         TGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGC                                            >  SRR19973066.3191382/1‑150 (MQ=60)
                                                                                                           GCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCC                                          <  SRR19973066.3293345/150‑1 (MQ=60)
                                                                                                             TGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCAT                                        >  SRR19973066.2573951/1‑150 (MQ=60)
                                                                                                             TGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCAT                                        >  SRR19973066.774254/1‑150 (MQ=60)
                                                                                                             TGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCAT                                        <  SRR19973066.288468/150‑1 (MQ=60)
                                                                                                             TGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCAT                                        <  SRR19973066.2919155/150‑1 (MQ=60)
                                                                                                               TTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCC                                      <  SRR19973066.2607114/150‑1 (MQ=60)
                                                                                                                TGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCT                                     <  SRR19973066.1304216/150‑1 (MQ=60)
                                                                                                                TGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCT                                     <  SRR19973066.113684/150‑1 (MQ=60)
                                                                                                                TGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCT                                     <  SRR19973066.584346/150‑1 (MQ=60)
                                                                                                                   AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                  <  SRR19973066.2239710/150‑1 (MQ=60)
                                                                                                                   AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                  <  SRR19973066.52115/150‑1 (MQ=60)
                                                                                                                    AACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGT                                 <  SRR19973066.3082431/150‑1 (MQ=60)
                                                                                                                    AACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGT                                 <  SRR19973066.3052564/150‑1 (MQ=60)
                                                                                                                     ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                <  SRR19973066.249789/150‑1 (MQ=60)
                                                                                                                     ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                <  SRR19973066.249778/150‑1 (MQ=60)
                                                                                                                     ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                >  SRR19973066.926283/1‑150 (MQ=60)
                                                                                                                     ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                <  SRR19973066.249781/150‑1 (MQ=60)
                                                                                                                     ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                <  SRR19973066.170523/150‑1 (MQ=60)
                                                                                                                      CCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGA                               <  SRR19973066.712813/150‑1 (MQ=60)
                                                                                                                       CTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAG                              >  SRR19973066.2905801/1‑150 (MQ=60)
                                                                                                                         CACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGAT                            >  SRR19973066.1214282/1‑150 (MQ=60)
                                                                                                                           CCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGA                          >  SRR19973066.1969850/1‑150 (MQ=60)
                                                                                                                            CCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAT                         >  SRR19973066.1118768/1‑150 (MQ=60)
                                                                                                                            CCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAT                         >  SRR19973066.1118817/1‑150 (MQ=60)
                                                                                                                            CCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAT                         >  SRR19973066.695138/1‑150 (MQ=60)
                                                                                                                            CCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAT                         >  SRR19973066.2239192/1‑150 (MQ=60)
                                                                                                                              CGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTG                       >  SRR19973066.312775/1‑150 (MQ=60)
                                                                                                                                        TGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTG             <  SRR19973066.1296343/150‑1 (MQ=60)
                                                                                                                                        TGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTG             <  SRR19973066.1296176/150‑1 (MQ=60)
                                                                                                                                        TGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTG             <  SRR19973066.2774619/150‑1 (MQ=60)
                                                                                                                                           GGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGT          <  SRR19973066.3442785/150‑1 (MQ=60)
                                                                                                                                           GGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGT          <  SRR19973066.3442815/150‑1 (MQ=60)
                                                                                                                                               CTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGG      >  SRR19973066.332355/1‑150 (MQ=60)
                                                                                                                                               CTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGG      >  SRR19973066.2189425/1‑150 (MQ=60)
                                                                                                                                                  CAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATG   >  SRR19973066.392426/1‑150 (MQ=60)
                                                                                                                                                  CAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATG   <  SRR19973066.1151779/150‑1 (MQ=60)
                                                                                                                                                  CAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATG   <  SRR19973066.1152694/150‑1 (MQ=60)
                                                                                                                                                   AGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGG  >  SRR19973066.2720144/1‑150 (MQ=60)
                                                                                                                                                   AGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGG  >  SRR19973066.539512/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGG  >  CP009273/1514709‑1515005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑