Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,514,858 G→A 100% E114K (GAA→AAA)  mcbR → colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,514,8580GA98.4% 842.5 / 2.4 255E114K (GAA→AAA) mcbRcolanic acid and biofilm gene transcriptional regulator, MqsR‑controlled
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (116/135);  total (117/138)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 255 total aligned reads displayed.

GTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA  >  CP009273/1514714‑1515002
                                                                                                                                                |                                                                                                                                                
gTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAAt                                                                                                                                               <  1:3029816/148‑1 (MQ=255)
    cACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCt                                                                                                                                           <  2:1104953/148‑1 (MQ=255)
     aCCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTc                                                                                                                                          <  1:2024752/148‑1 (MQ=255)
         gCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCgaga                                                                                                                                      <  2:1268268/148‑1 (MQ=255)
           acaAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGaaa                                                                                                                                    >  2:3164581/1‑148 (MQ=255)
                   ttACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAcagca                                                                                                                            >  2:1777279/1‑148 (MQ=255)
                     aCAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAgg                                                                                                                          <  1:3232269/148‑1 (MQ=255)
                       aGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGgcg                                                                                                                        <  1:1333988/148‑1 (MQ=255)
                         ttCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                                      >  2:664614/1‑148 (MQ=255)
                         ttCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                                      <  1:501884/148‑1 (MQ=255)
                              gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaa                                                                                                                 <  2:2886918/148‑1 (MQ=255)
                               aaGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa                                                                                                                >  2:3445098/1‑148 (MQ=255)
                               aaGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa                                                                                                                >  1:2649978/1‑148 (MQ=255)
                               aaGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa                                                                                                                <  1:2372013/148‑1 (MQ=255)
                                aGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAg                                                                                                               <  1:2453556/148‑1 (MQ=255)
                                aGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAg                                                                                                               <  2:1343924/148‑1 (MQ=255)
                                aGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAg                                                                                                               <  2:2508178/148‑1 (MQ=255)
                                    gggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCg                                                                                                           >  1:464210/1‑148 (MQ=255)
                                      gAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAc                                                                                                         >  1:916894/1‑148 (MQ=255)
                                         aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg                                                                                                      <  2:2089921/148‑1 (MQ=255)
                                         aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg                                                                                                      >  2:1936606/1‑148 (MQ=255)
                                         aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg                                                                                                      >  1:1498126/1‑148 (MQ=255)
                                         aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg                                                                                                      <  1:3164628/148‑1 (MQ=255)
                                           gTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGa                                                                                                    <  1:416764/148‑1 (MQ=255)
                                                ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTATTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaat                                                                                               <  2:3271237/148‑2 (MQ=255)
                                                ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                               >  2:2239907/1‑148 (MQ=255)
                                                ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                               >  2:2968679/1‑148 (MQ=255)
                                                   gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                                            <  1:2561169/148‑1 (MQ=255)
                                                       aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                                        >  2:16827/1‑148 (MQ=255)
                                                          tcaatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGt                                                                                     >  1:2535158/1‑148 (MQ=255)
                                                          tcaatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGt                                                                                     <  2:1421197/148‑1 (MQ=255)
                                                               cGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACa                                                                                <  2:2716263/148‑1 (MQ=255)
                                                               cGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACa                                                                                <  1:1469903/148‑1 (MQ=255)
                                                                ggATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAg                                                                               <  1:923895/148‑1 (MQ=255)
                                                                 gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                              <  1:1415949/148‑1 (MQ=255)
                                                                 gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                              >  1:888773/1‑148 (MQ=255)
                                                                  aTCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGc                                                                             <  2:1404180/148‑1 (MQ=255)
                                                                    ccGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTa                                                                           <  1:3450992/148‑1 (MQ=255)
                                                                       cTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAttt                                                                        >  2:2603406/1‑148 (MQ=255)
                                                                       cTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAttt                                                                        >  2:2684155/1‑148 (MQ=255)
                                                                        tACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTc                                                                       >  2:936369/1‑148 (MQ=255)
                                                                         aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg                                                                      >  1:681571/1‑148 (MQ=255)
                                                                         aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg                                                                      <  1:332747/148‑1 (MQ=255)
                                                                          cGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGc                                                                     >  1:2219578/1‑148 (MQ=255)
                                                                          cGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGc                                                                     <  2:532727/148‑1 (MQ=255)
                                                                          cGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGc                                                                     <  1:1040522/148‑1 (MQ=255)
                                                                            aaCGGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCtt                                                                   <  2:1815303/148‑1 (MQ=255)
                                                                             aCTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTa                                                                  <  1:2403666/148‑1 (MQ=255)
                                                                              cTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAg                                                                 <  1:881675/148‑1 (MQ=255)
                                                                                 gAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGa                                                              >  1:1882933/1‑148 (MQ=255)
                                                                                 gAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGa                                                              <  2:2649978/148‑1 (MQ=255)
                                                                                 gAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGa                                                              <  1:590496/148‑1 (MQ=255)
                                                                                  aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                                             <  1:664614/148‑1 (MQ=255)
                                                                                  aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                                             <  1:728702/148‑1 (MQ=255)
                                                                                   aTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt                                                            <  2:2364210/148‑1 (MQ=255)
                                                                                   aTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt                                                            <  1:2926122/148‑1 (MQ=255)
                                                                                    ttAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt                                                           >  2:1565296/1‑148 (MQ=255)
                                                                                     tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa                                                          <  2:1751727/148‑1 (MQ=255)
                                                                                      aaTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTat                                                         <  1:2312970/148‑1 (MQ=255)
                                                                                       aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGTAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        <  1:2163635/148‑1 (MQ=255)
                                                                                       aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        <  1:1196294/148‑1 (MQ=255)
                                                                                       aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        <  2:470771/148‑1 (MQ=255)
                                                                                       aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                                        <  1:1932993/148‑1 (MQ=255)
                                                                                          gCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcatc                                                     <  2:1533851/148‑1 (MQ=255)
                                                                                             gTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCt                                                  <  2:807753/148‑1 (MQ=255)
                                                                                             gTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCt                                                  <  2:1867446/148‑1 (MQ=255)
                                                                                              ttGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                                 >  2:2873871/1‑148 (MQ=255)
                                                                                              ttGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                                 >  1:1787986/1‑148 (MQ=255)
                                                                                              ttGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                                 >  1:548962/1‑148 (MQ=255)
                                                                                                      gCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGc                                         <  2:1128218/148‑1 (MQ=255)
                                                                                                      gCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGc                                         >  1:753856/1‑148 (MQ=255)
                                                                                                       cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc                                        >  2:3480397/1‑148 (MQ=255)
                                                                                                        tGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGccc                                       <  1:1632304/148‑1 (MQ=255)
                                                                                                        tGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGccc                                       <  2:2289721/148‑1 (MQ=255)
                                                                                                              aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                                 <  1:681304/148‑1 (MQ=255)
                                                                                                              aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                                 <  1:681019/148‑1 (MQ=255)
                                                                                                              aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATAAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                                 >  1:1092346/1‑148 (MQ=255)
                                                                                                              aaaCATCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                                 >  1:2873481/1‑148 (MQ=255)
                                                                                                               aaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgt                                >  1:2820255/1‑148 (MQ=255)
                                                                                                                aCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtg                               <  1:3334554/148‑1 (MQ=255)
                                                                                                                  cTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgtg                             <  1:1876127/148‑1 (MQ=255)
                                                                                                                     aCCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga                          <  2:1194500/148‑1 (MQ=255)
                                                                                                                      ccccGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgat                         >  2:914523/1‑148 (MQ=255)
                                                                                                                        ccGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatga                       >  1:3426320/1‑148 (MQ=255)
                                                                                                                        ccGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatga                       >  2:2959420/1‑148 (MQ=255)
                                                                                                                           cAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTg                    >  1:1007235/1‑148 (MQ=255)
                                                                                                                            aaGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGa                   >  2:1970063/1‑148 (MQ=255)
                                                                                                                            aaGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGa                   <  1:2334567/148‑1 (MQ=255)
                                                                                                                                ccTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCaa               >  2:57124/1‑148 (MQ=255)
                                                                                                                                   tGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtg            >  2:3014880/1‑148 (MQ=255)
                                                                                                                                   tGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtg            <  1:2956000/148‑1 (MQ=255)
                                                                                                                                   tGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtg            >  2:1021749/1‑148 (MQ=255)
                                                                                                                                     cGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtgtg          <  2:145506/148‑1 (MQ=255)
                                                                                                                                       gAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTggg        >  1:2498526/1‑148 (MQ=255)
                                                                                                                                        aaCTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGt       >  2:2405247/1‑148 (MQ=255)
                                                                                                                                         aCTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTc      <  1:935875/148‑1 (MQ=255)
                                                                                                                                          cTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCa     <  1:1637294/148‑1 (MQ=255)
                                                                                                                                           tCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAAGTAAACAGGCTATTTCGCTTAGCGAGTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAg    >  2:309686/1‑148 (MQ=255)
                                                                                                                                            ccAGAAATTGCTCGAGATATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAgg   <  2:3198410/148‑1 (MQ=255)
                                                                                                                                             cAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGa  <  2:2102924/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA  >  CP009273/1514714‑1515002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGG  >  CP009273/1514712‑1515007
                                                                                                                                                  |                                                                                                                                                     
CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAAT                                                                                                                                                    <  SRR19973065.3312267/150‑1 (MQ=60)
CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAAT                                                                                                                                                    <  SRR19973065.1601572/150‑1 (MQ=60)
  GTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTG                                                                                                                                                  <  SRR19973065.3048379/150‑1 (MQ=60)
     GCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGACCTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTC                                                                                                                                               <  SRR19973065.2083662/150‑1 (MQ=60)
      CACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCG                                                                                                                                              >  SRR19973065.1891622/1‑150 (MQ=60)
       ACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGA                                                                                                                                             <  SRR19973065.250919/150‑1 (MQ=60)
       ACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGA                                                                                                                                             <  SRR19973065.2037768/150‑1 (MQ=60)
         CTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGA                                                                                                                                           <  SRR19973065.893053/150‑1 (MQ=60)
              CAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTA                                                                                                                                      >  SRR19973065.2393047/1‑150 (MQ=60)
              CAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTA                                                                                                                                      >  SRR19973065.2686256/1‑150 (MQ=60)
                   ANTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCA                                                                                                                                 >  SRR19973065.1391681/1‑150 (MQ=60)
                      TACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGC                                                                                                                              <  SRR19973065.95550/150‑1 (MQ=60)
                       ACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCG                                                                                                                             <  SRR19973065.3251701/150‑1 (MQ=60)
                         AGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAGAGCAGGCGCA                                                                                                                           <  SRR19973065.1909685/150‑1 (MQ=60)
                         AGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCA                                                                                                                           <  SRR19973065.1342822/150‑1 (MQ=60)
                           TTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAG                                                                                                                         <  SRR19973065.505244/150‑1 (MQ=60)
                              CGGAAGTGGGGAAACGTCAATTAGACTAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAA                                                                                                                      >  SRR19973065.2558229/1‑150 (MQ=60)
                              CGGAAGTGGGGAAACGTCAATTAGACTAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAA                                                                                                                      >  SRR19973065.2558644/1‑150 (MQ=60)
                               GGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAA                                                                                                                     >  SRR19973065.2666318/1‑150 (MQ=60)
                               GGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAA                                                                                                                     >  SRR19973065.2920082/1‑150 (MQ=60)
                                 AAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGG                                                                                                                   <  SRR19973065.2386817/150‑1 (MQ=60)
                                 AAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGG                                                                                                                   <  SRR19973065.1771612/150‑1 (MQ=60)
                                 AAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGG                                                                                                                   <  SRR19973065.3517408/150‑1 (MQ=60)
                                  AGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGG                                                                                                                  <  SRR19973065.2468967/150‑1 (MQ=60)
                                  AGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGG                                                                                                                  <  SRR19973065.2469017/150‑1 (MQ=60)
                                    TGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCG                                                                                                                >  SRR19973065.467414/1‑150 (MQ=60)
                                    TGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCG                                                                                                                >  SRR19973065.467410/1‑150 (MQ=60)
                                    TGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCG                                                                                                                >  SRR19973065.467436/1‑150 (MQ=60)
                                      GGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCTCAAGAAAAGGGCGAC                                                                                                              >  SRR19973065.924450/1‑150 (MQ=60)
                                      GGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAC                                                                                                              >  SRR19973065.922883/1‑150 (MQ=60)
                                       GGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACA                                                                                                             <  SRR19973065.1202827/150‑1 (MQ=60)
                                         AAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATG                                                                                                           >  SRR19973065.1507774/1‑150 (MQ=60)
                                          AACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGG                                                                                                          >  SRR19973065.476614/1‑150 (MQ=60)
                                          AACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGG                                                                                                          >  SRR19973065.3412060/1‑150 (MQ=60)
                                           ACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGA                                                                                                         <  SRR19973065.3181354/150‑1 (MQ=60)
                                           ACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGA                                                                                                         <  SRR19973065.3183797/150‑1 (MQ=60)
                                             GTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAAC                                                                                                       <  SRR19973065.419682/150‑1 (MQ=60)
                                               CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAA                                                                                                     >  SRR19973065.1644171/1‑150 (MQ=60)
                                                AATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAA                                                                                                    >  SRR19973065.901631/1‑150 (MQ=60)
                                                 ATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAAT                                                                                                   <  SRR19973065.2967275/150‑1 (MQ=60)
                                                 ATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAAT                                                                                                   <  SRR19973065.1206754/150‑1 (MQ=60)
                                                  TTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATC                                                                                                  >  SRR19973065.1527123/1‑150 (MQ=60)
                                                   TAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCA                                                                                                 <  SRR19973065.2504832/150‑1 (MQ=60)
                                                     GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                               <  SRR19973065.2577604/150‑1 (MQ=60)
                                                     GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                               <  SRR19973065.2577181/150‑1 (MQ=60)
                                                       CGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAA                                                                                             >  SRR19973065.1033211/1‑150 (MQ=60)
                                                          AATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGT                                                                                          >  SRR19973065.2551063/1‑150 (MQ=60)
                                                               ATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACA                                                                                     <  SRR19973065.1586371/150‑1 (MQ=60)
                                                                 CGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGG                                                                                   <  SRR19973065.1479553/150‑1 (MQ=60)
                                                                 CGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGG                                                                                   <  SRR19973065.1479325/150‑1 (MQ=60)
                                                                 CGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGG                                                                                   >  SRR19973065.894413/1‑150 (MQ=60)
                                                                 CGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGG                                                                                   >  SRR19973065.2282966/1‑150 (MQ=60)
                                                                 CGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGG                                                                                   >  SRR19973065.2232415/1‑150 (MQ=60)
                                                                  GGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGC                                                                                  <  SRR19973065.929930/150‑1 (MQ=60)
                                                                  GGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGC                                                                                  <  SRR19973065.931958/150‑1 (MQ=60)
                                                                  GGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGC                                                                                  <  SRR19973065.929956/150‑1 (MQ=60)
                                                                   GATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCT                                                                                 <  SRR19973065.235255/150‑1 (MQ=60)
                                                                   GATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCT                                                                                 <  SRR19973065.1425199/150‑1 (MQ=60)
                                                                      CCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATT                                                                              <  SRR19973065.3472033/150‑1 (MQ=60)
                                                                         CTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCG                                                                           >  SRR19973065.686066/1‑150 (MQ=60)
                                                                         CTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCG                                                                           >  SRR19973065.686105/1‑150 (MQ=60)
                                                                          TACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGC                                                                          >  SRR19973065.2233746/1‑150 (MQ=60)
                                                                           ACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCT                                                                         <  SRR19973065.335073/150‑1 (MQ=60)
                                                                           ACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCT                                                                         <  SRR19973065.334890/150‑1 (MQ=60)
                                                                           ACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCT                                                                         <  SRR19973065.334810/150‑1 (MQ=60)
                                                                            CGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTT                                                                        >  SRR19973065.728585/1‑150 (MQ=60)
                                                                            CGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTT                                                                        >  SRR19973065.3240588/1‑150 (MQ=60)
                                                                            CGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTT                                                                        <  SRR19973065.1047410/150‑1 (MQ=60)
                                                                              AACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAG                                                                      <  SRR19973065.2900949/150‑1 (MQ=60)
                                                                               ACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGC                                                                     <  SRR19973065.2418780/150‑1 (MQ=60)
                                                                                CTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCG                                                                    <  SRR19973065.887274/150‑1 (MQ=60)
                                                                                CTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCG                                                                    <  SRR19973065.887291/150‑1 (MQ=60)
                                                                                CTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCG                                                                    >  SRR19973065.1867080/1‑150 (MQ=60)
                                                                                 TGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGA                                                                   >  SRR19973065.1895699/1‑150 (MQ=60)
                                                                                 TGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGA                                                                   >  SRR19973065.1895329/1‑150 (MQ=60)
                                                                                  GGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCTAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAT                                                                  >  SRR19973065.3348164/1‑150 (MQ=60)
                                                                                   GAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATT                                                                 >  SRR19973065.3038298/1‑150 (MQ=60)
                                                                                   GAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATT                                                                 >  SRR19973065.3038301/1‑150 (MQ=60)
                                                                                   GAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATT                                                                 <  SRR19973065.594744/150‑1 (MQ=60)
                                                                                   GAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATT                                                                 <  SRR19973065.594155/150‑1 (MQ=60)
                                                                                    AATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTT                                                                <  SRR19973065.2294422/150‑1 (MQ=60)
                                                                                    AATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTT                                                                <  SRR19973065.733582/150‑1 (MQ=60)
                                                                                    AATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTT                                                                <  SRR19973065.669026/150‑1 (MQ=60)
                                                                                    AATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTT                                                                <  SRR19973065.669025/150‑1 (MQ=60)
                                                                                    AATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTT                                                                <  SRR19973065.668604/150‑1 (MQ=60)
                                                                                     ATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTA                                                               <  SRR19973065.2943897/150‑1 (MQ=60)
                                                                                     ATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTA                                                               <  SRR19973065.3084470/150‑1 (MQ=60)
                                                                                       TAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAAAGGCTATTTCGCTTAGCGATTTATC                                                             <  SRR19973065.275340/150‑1 (MQ=60)
                                                                                        AATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCA                                                            <  SRR19973065.2327483/150‑1 (MQ=60)
                                                                                         ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGTAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                           <  SRR19973065.2177540/150‑1 (MQ=60)
                                                                                         ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                           <  SRR19973065.1203916/150‑1 (MQ=60)
                                                                                         ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                           <  SRR19973065.1204093/150‑1 (MQ=60)
                                                                                         ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                           <  SRR19973065.3369351/150‑1 (MQ=60)
                                                                                         ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                           <  SRR19973065.1945647/150‑1 (MQ=60)
                                                                                          TGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATC                                                          >  SRR19973065.518197/1‑150 (MQ=60)
                                                                                              AGTTGCTCTGGCTGTTGAAAACCTCACCCCGCGAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTC                                                      >  SRR19973065.1800363/1‑150 (MQ=60)
                                                                                              AGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTC                                                      >  SRR19973065.552497/1‑150 (MQ=60)
                                                                                              AGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTC                                                      >  SRR19973065.2516204/1‑150 (MQ=60)
                                                                                              AGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTC                                                      >  SRR19973065.1799694/1‑150 (MQ=60)
                                                                                              AGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTC                                                      >  SRR19973065.170006/1‑150 (MQ=60)
                                                                                                 TGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAA                                                   >  SRR19973065.377674/1‑150 (MQ=60)
                                                                                                     CTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATG                                               <  SRR19973065.286172/150‑1 (MQ=60)
                                                                                                      TGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGC                                              >  SRR19973065.758884/1‑150 (MQ=60)
                                                                                                      TGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGC                                              >  SRR19973065.2411373/1‑150 (MQ=60)
                                                                                                      TGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGC                                              >  SRR19973065.3465885/1‑150 (MQ=60)
                                                                                                       GGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCC                                             >  SRR19973065.728803/1‑150 (MQ=60)
                                                                                                         CTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCA                                           >  SRR19973065.1459487/1‑150 (MQ=60)
                                                                                                          TGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCAT                                          <  SRR19973065.1643258/150‑1 (MQ=60)
                                                                                                             TGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCT                                       >  SRR19973065.743933/1‑150 (MQ=60)
                                                                                                             TGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCT                                       >  SRR19973065.216302/1‑150 (MQ=60)
                                                                                                              GAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTG                                      >  SRR19973065.1098709/1‑150 (MQ=60)
                                                                                                              GAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTG                                      >  SRR19973065.1098825/1‑150 (MQ=60)
                                                                                                              GAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATAAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTG                                      >  SRR19973065.1099539/1‑150 (MQ=60)
                                                                                                              GAAAACATCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCATG                                      >  SRR19973065.2891088/1‑150 (MQ=60)
                                                                                                               AAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGT                                     >  SRR19973065.2837678/1‑150 (MQ=60)
                                                                                                                AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                    <  SRR19973065.685551/150‑1 (MQ=60)
                                                                                                                AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                    <  SRR19973065.685649/150‑1 (MQ=60)
                                                                                                                AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                    <  SRR19973065.685753/150‑1 (MQ=60)
                                                                                                                AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                    <  SRR19973065.685837/150‑1 (MQ=60)
                                                                                                                  ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                  <  SRR19973065.3354912/150‑1 (MQ=60)
                                                                                                                  ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                  <  SRR19973065.82352/150‑1 (MQ=60)
                                                                                                                  ACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTG                                  <  SRR19973065.2357386/150‑1 (MQ=60)
                                                                                                                    CTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAG                                <  SRR19973065.1888480/150‑1 (MQ=60)
                                                                                                                    CTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAG                                >  SRR19973065.2339976/1‑150 (MQ=60)
                                                                                                                       ACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATG                             <  SRR19973065.52135/150‑1 (MQ=60)
                                                                                                                        CCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGA                            >  SRR19973065.3447229/1‑150 (MQ=60)
                                                                                                                           CGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTG                         >  SRR19973065.1013920/1‑150 (MQ=60)
                                                                                                                              AAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGC                      >  SRR19973065.592247/1‑150 (MQ=60)
                                                                                                                              AAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGC                      <  SRR19973065.2349150/150‑1 (MQ=60)
                                                                                                                                 ACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATTATTGAGCAAC                   >  SRR19973065.1474019/1‑150 (MQ=60)
                                                                                                                                  CCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACT                  <  SRR19973065.912898/150‑1 (MQ=60)
                                                                                                                                     TGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTG               <  SRR19973065.2974037/150‑1 (MQ=60)
                                                                                                                                       CGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGG             >  SRR19973065.2514241/1‑150 (MQ=60)
                                                                                                                                         GAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTC           <  SRR19973065.1960149/150‑1 (MQ=60)
                                                                                                                                           ACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAG         <  SRR19973065.942059/150‑1 (MQ=60)
                                                                                                                                           ACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAG         <  SRR19973065.942556/150‑1 (MQ=60)
                                                                                                                                            CTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGG        <  SRR19973065.1648287/150‑1 (MQ=60)
                                                                                                                                                  AAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGG  <  SRR19973065.2390778/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGG  >  CP009273/1514712‑1515007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑