Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,514,858 G→A 100% E114K (GAA→AAA)  mcbR → colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,514,8580GA95.7% 820.1 / 20.8 253E114K (GAA→AAA) mcbRcolanic acid and biofilm gene transcriptional regulator, MqsR‑controlled
Reads supporting (aligned to +/- strand):  ref base G (8/3);  new base A (90/152);  total (98/155)
Fisher's exact test for biased strand distribution p-value = 2.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 255 total aligned reads displayed.

CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA  >  CP009273/1514712‑1515002
                                                                                                                                                  |                                                                                                                                                
 tGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGaaa                                                                                                                                                <  2:3445535/148‑1 (MQ=255)
 tGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGaaa                                                                                                                                                <  1:504021/148‑1 (MQ=255)
     gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACTAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGc                                                                                                                                            >  2:1158343/1‑148 (MQ=255)
     gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGc                                                                                                                                            <  2:3097070/148‑1 (MQ=255)
     gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGc                                                                                                                                            <  2:1327196/148‑1 (MQ=255)
        ccTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCg                                                                                                                                         >  2:1162159/1‑148 (MQ=255)
         cTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCga                                                                                                                                        <  1:3399356/148‑1 (MQ=255)
          tGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCgag                                                                                                                                       <  1:3269394/148‑1 (MQ=255)
             acaAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGaaa                                                                                                                                    <  2:3162984/148‑1 (MQ=255)
              caAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAAt                                                                                                                                   >  1:1452763/1‑148 (MQ=255)
               aaGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAAtt                                                                                                                                  <  1:1048518/148‑1 (MQ=255)
                    tttACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAcagc                                                                                                                             <  2:1237674/148‑1 (MQ=255)
                      tACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAcagcag                                                                                                                           >  1:913024/1‑148 (MQ=255)
                           ttCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                                      <  2:3362155/148‑1 (MQ=255)
                           ttCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                                      >  1:2022439/1‑148 (MQ=255)
                            tCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGATACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCaa                                                                                                                     <  2:601234/148‑1 (MQ=255)
                               ggAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGAACTTGCGGAACTCCAGAAATTGATCGAGAAATTACAGCAGGCGCAAGaa                                                                                                                  >  1:2425416/1‑148 (MQ=255)
                                gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaa                                                                                                                 <  1:641234/148‑1 (MQ=255)
                                gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaa                                                                                                                 <  2:1694081/148‑1 (MQ=255)
                                   gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg                                                                                                              >  1:254041/1‑148 (MQ=255)
                                   gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg                                                                                                              <  2:1984575/148‑1 (MQ=255)
                                   gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCAGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg                                                                                                              <  2:687752/148‑1 (MQ=255)
                                    tGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAggg                                                                                                             >  2:1218943/1‑148 (MQ=255)
                                     ggggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGc                                                                                                            <  1:3047882/148‑1 (MQ=255)
                                       ggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGa                                                                                                          >  2:2138507/1‑148 (MQ=255)
                                        gAAACGTCAATGAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAc                                                                                                         <  1:1863836/148‑1 (MQ=255)
                                           aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg                                                                                                      >  2:467351/1‑148 (MQ=255)
                                            cGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATgg                                                                                                     >  1:833864/1‑148 (MQ=255)
                                              tCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGaa                                                                                                   >  2:341465/1‑148 (MQ=255)
                                                 aTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaa                                                                                                >  1:2789713/1‑148 (MQ=255)
                                                 aTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaa                                                                                                <  2:2647130/148‑1 (MQ=255)
                                                  ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                               >  2:2854443/1‑148 (MQ=255)
                                                   tAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAAt                                                                                              <  1:2192996/148‑1 (MQ=255)
                                                     gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                                            <  1:1162159/148‑1 (MQ=255)
                                                     gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                                            <  1:2201115/148‑1 (MQ=255)
                                                        gAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTa                                                                                         <  1:2280366/148‑1 (MQ=255)
                                                         aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                                        <  2:246138/148‑1 (MQ=255)
                                                         aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                                        <  2:2247151/148‑1 (MQ=255)
                                                         aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                                        <  2:1315189/148‑1 (MQ=255)
                                                          aatcaatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAt                                                                                       <  1:611701/148‑1 (MQ=255)
                                                          aatcaatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAt                                                                                       <  2:1815634/148‑1 (MQ=255)
                                                             caatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTa                                                                                    <  2:531323/148‑1 (MQ=255)
                                                               atcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTaaa                                                                                  <  1:1038912/148‑1 (MQ=255)
                                                                tcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAc                                                                                 <  1:3002327/148‑1 (MQ=255)
                                                                  ggATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAg                                                                               >  2:587175/1‑148 (MQ=255)
                                                                  ggATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAg                                                                               >  2:887976/1‑148 (MQ=255)
                                                                   gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                              >  2:2504265/1‑148 (MQ=255)
                                                                   gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                              <  2:1770012/148‑1 (MQ=255)
                                                                    aTCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGc                                                                             <  1:1929654/148‑1 (MQ=255)
                                                                      ccGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTa                                                                           <  1:443629/148‑1 (MQ=255)
                                                                        tcTACGAACTGGAATTAATTGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAtt                                                                         <  2:3337810/147‑1 (MQ=255)
                                                                        gCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAtt                                                                         <  1:157158/148‑1 (MQ=255)
                                                                         cTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAttt                                                                        <  1:1904266/148‑1 (MQ=255)
                                                                           aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGATATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg                                                                      <  1:3421663/148‑1 (MQ=255)
                                                                           aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg                                                                      >  2:3320789/1‑148 (MQ=255)
                                                                             gAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAACAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt                                                                    <  2:2022448/148‑1 (MQ=255)
                                                                             gAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt                                                                    <  2:2737900/148‑1 (MQ=255)
                                                                              aaCTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCtt                                                                   <  1:1339033/148‑1 (MQ=255)
                                                                                    aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                                             >  2:3494359/1‑148 (MQ=255)
                                                                                    aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                                             >  1:3506624/1‑148 (MQ=255)
                                                                                     aTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt                                                            >  1:1254041/1‑148 (MQ=255)
                                                                                      ttAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt                                                           >  1:2036916/1‑148 (MQ=255)
                                                                                      ttAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt                                                           <  2:1418795/148‑1 (MQ=255)
                                                                                       tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa                                                          <  2:1145178/148‑1 (MQ=255)
                                                                                       tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa                                                          >  1:722268/1‑148 (MQ=255)
                                                                                          tGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatca                                                       <  1:290057/148‑1 (MQ=255)
                                                                                             cAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCg                                                    <  2:867562/148‑1 (MQ=255)
                                                                                             cAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCg                                                    <  2:2589440/148‑1 (MQ=255)
                                                                                              aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGc                                                   >  2:2413874/1‑148 (MQ=255)
                                                                                              aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCTCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGc                                                   >  2:3239975/1‑148 (MQ=255)
                                                                                              aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGc                                                   <  1:920998/148‑1 (MQ=255)
                                                                                                ttGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                                 >  1:1690969/1‑148 (MQ=255)
                                                                                                         cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc                                        <  2:1846316/148‑1 (MQ=255)
                                                                                                             tGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATc                                    >  2:1968776/1‑148 (MQ=255)
                                                                                                             tGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATc                                    >  2:176448/1‑148 (MQ=255)
                                                                                                               aaaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCt                                  <  1:717557/148‑1 (MQ=255)
                                                                                                                aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                                 >  2:2015026/1‑148 (MQ=255)
                                                                                                                aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                                 <  1:2994502/148‑1 (MQ=255)
                                                                                                                     tCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTga                            <  1:896133/148‑1 (MQ=255)
                                                                                                                      cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag                           <  2:3455814/148‑1 (MQ=255)
                                                                                                                      cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag                           <  2:913024/148‑1 (MQ=255)
                                                                                                                      cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag                           <  1:2333304/148‑1 (MQ=255)
                                                                                                                      cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag                           <  2:1694591/148‑1 (MQ=255)
                                                                                                                       aCCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga                          >  2:25615/1‑148 (MQ=255)
                                                                                                                         cccGCAAGACCTTGCGGAACTCCAGATATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatg                        <  2:73950/148‑1 (MQ=255)
                                                                                                                           cGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatgat                      >  2:102655/1‑148 (MQ=255)
                                                                                                                           cGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTGATCATCGCTCAAATATGACCATCCTGTGTGAgatgat                      >  2:3294553/1‑148 (MQ=255)
                                                                                                                            gCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAtt                     >  2:400676/1‑148 (MQ=255)
                                                                                                                             cAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTg                    <  2:1382822/148‑1 (MQ=255)
                                                                                                                                gACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGc                 >  1:3297720/1‑148 (MQ=255)
                                                                                                                                  ccTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCaa               >  2:1426768/1‑148 (MQ=255)
                                                                                                                                   cTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAAc              >  2:1568576/1‑148 (MQ=255)
                                                                                                                                   cTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAAc              >  2:2813847/1‑148 (MQ=255)
                                                                                                                                       cGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtgtg          <  1:1218943/148‑1 (MQ=255)
                                                                                                                                         gAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTggg        <  2:1199301/148‑1 (MQ=255)
                                                                                                                                           aCTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTc      <  2:1795642/148‑1 (MQ=255)
                                                                                                                                            cTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCa     >  2:3141538/1‑148 (MQ=255)
                                                                                                                                            cTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCa     >  1:773347/1‑148 (MQ=255)
                                                                                                                                            cTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCa     >  1:163271/1‑148 (MQ=255)
                                                                                                                                               cAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGa  <  2:841451/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA  >  CP009273/1514712‑1515002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGG  >  CP009273/1514709‑1515007
                                                                                                                                                     |                                                                                                                                                     
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGA                                                                                                                                                       >  SRR19973067.3559456/1‑150 (MQ=60)
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGA                                                                                                                                                       >  SRR19973067.3277388/1‑150 (MQ=60)
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGA                                                                                                                                                       >  SRR19973067.2077725/1‑150 (MQ=60)
   CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAAT                                                                                                                                                    <  SRR19973067.1052246/150‑1 (MQ=60)
    TGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATT                                                                                                                                                   <  SRR19973067.507473/150‑1 (MQ=60)
          ACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGA                                                                                                                                             >  SRR19973067.2002504/1‑150 (MQ=60)
            CTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGA                                                                                                                                           <  SRR19973067.3420999/150‑1 (MQ=60)
             TGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAA                                                                                                                                          <  SRR19973067.3289810/150‑1 (MQ=60)
             TGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAA                                                                                                                                          <  SRR19973067.529127/150‑1 (MQ=60)
               CACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAAT                                                                                                                                        >  SRR19973067.1462490/1‑150 (MQ=60)
                  AAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAC                                                                                                                                     <  SRR19973067.616018/150‑1 (MQ=60)
                  AAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAC                                                                                                                                     <  SRR19973067.1055715/150‑1 (MQ=60)
                  AAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAC                                                                                                                                     <  SRR19973067.1575063/150‑1 (MQ=60)
                     CATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGC                                                                                                                                  <  SRR19973067.911804/150‑1 (MQ=60)
                       TTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAG                                                                                                                                >  SRR19973067.919105/1‑150 (MQ=60)
                       TTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAG                                                                                                                                <  SRR19973067.424205/150‑1 (MQ=60)
                       TTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAG                                                                                                                                <  SRR19973067.425789/150‑1 (MQ=60)
                       TTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAG                                                                                                                                <  SRR19973067.50988/150‑1 (MQ=60)
                       TTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAG                                                                                                                                >  SRR19973067.919102/1‑150 (MQ=60)
                            AGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCA                                                                                                                           >  SRR19973067.2036018/1‑150 (MQ=60)
                            AGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCA                                                                                                                           >  SRR19973067.2036027/1‑150 (MQ=60)
                            AGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCA                                                                                                                           >  SRR19973067.3398342/1‑150 (MQ=60)
                                CCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGAACTTGCGGAACTCCAGAAATTGATCGAGAAATTACAGCAGGCGCAAGAA                                                                                                                       >  SRR19973067.2441330/1‑150 (MQ=60)
                                   GAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAG                                                                                                                    <  SRR19973067.645552/150‑1 (MQ=60)
                                    AAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGG                                                                                                                   >  SRR19973067.255781/1‑150 (MQ=60)
                                        GGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGA                                                                                                               <  SRR19973067.3067298/150‑1 (MQ=60)
                                        GGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGA                                                                                                               <  SRR19973067.3067293/150‑1 (MQ=60)
                                           GAAACGTCAATGAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACAT                                                                                                            <  SRR19973067.1876550/150‑1 (MQ=60)
                                            AAACGTCAATTGGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATG                                                                                                           >  SRR19973067.1994243/1‑150 (MQ=60)
                                            AAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATG                                                                                                           <  SRR19973067.1647542/150‑1 (MQ=60)
                                             AACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGG                                                                                                          >  SRR19973067.839538/1‑150 (MQ=60)
                                                 TCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACA                                                                                                      <  SRR19973067.173318/150‑1 (MQ=60)
                                                  CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAA                                                                                                     >  SRR19973067.2807835/1‑150 (MQ=60)
                                                  CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAA                                                                                                     >  SRR19973067.1877950/1‑150 (MQ=60)
                                                  CAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAA                                                                                                     >  SRR19973067.2807710/1‑150 (MQ=60)
                                                      TAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCA                                                                                                 <  SRR19973067.2207626/150‑1 (MQ=60)
                                                      TAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCA                                                                                                 <  SRR19973067.2207652/150‑1 (MQ=60)
                                                       AGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAT                                                                                                <  SRR19973067.1845249/150‑1 (MQ=60)
                                                        GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                               <  SRR19973067.2215798/150‑1 (MQ=60)
                                                        GACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATT                                                                                               <  SRR19973067.1169891/150‑1 (MQ=60)
                                                           GAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAT                                                                                            <  SRR19973067.2295442/150‑1 (MQ=60)
                                                           GAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAT                                                                                            <  SRR19973067.2295447/150‑1 (MQ=60)
                                                             AATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGT                                                                                          >  SRR19973067.3233793/1‑150 (MQ=60)
                                                             AATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGT                                                                                          <  SRR19973067.615663/150‑1 (MQ=60)
                                                                 AATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAC                                                                                      <  SRR19973067.2530926/150‑1 (MQ=60)
                                                                  ATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACA                                                                                     <  SRR19973067.1046060/150‑1 (MQ=60)
                                                                   TCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAG                                                                                    <  SRR19973067.3021388/150‑1 (MQ=60)
                                                                   TCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAG                                                                                    <  SRR19973067.83158/150‑1 (MQ=60)
                                                                   TCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAG                                                                                    <  SRR19973067.2631196/150‑1 (MQ=60)
                                                                   TCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAG                                                                                    <  SRR19973067.2425201/150‑1 (MQ=60)
                                                                     GGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGC                                                                                  <  SRR19973067.2131610/150‑1 (MQ=60)
                                                                       ATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTA                                                                                <  SRR19973067.133349/150‑1 (MQ=60)
                                                                       ATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTA                                                                                <  SRR19973067.1942822/150‑1 (MQ=60)
                                                                         CCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATT                                                                              <  SRR19973067.2292035/150‑1 (MQ=60)
                                                                         CCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATT                                                                              <  SRR19973067.446705/150‑1 (MQ=60)
                                                                           GCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTC                                                                            <  SRR19973067.158722/150‑1 (MQ=60)
                                                                           GCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTC                                                                            <  SRR19973067.158472/150‑1 (MQ=60)
                                                                           GCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTC                                                                            <  SRR19973067.1232635/150‑1 (MQ=60)
                                                                            CTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCG                                                                           <  SRR19973067.1917250/150‑1 (MQ=60)
                                                                              ACGTACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGATATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCT                                                                         <  SRR19973067.3443437/150‑1 (MQ=60)
                                                                               CGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTT                                                                        <  SRR19973067.1615326/150‑1 (MQ=60)
                                                                                 AACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAG                                                                      <  SRR19973067.1348196/150‑1 (MQ=60)
                                                                                 AACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATGACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAG                                                                      <  SRR19973067.1348214/150‑1 (MQ=60)
                                                                                     GGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAT                                                                  >  SRR19973067.3528837/1‑150 (MQ=60)
                                                                                      GAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATT                                                                 >  SRR19973067.1262524/1‑150 (MQ=60)
                                                                                       AATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTT                                                                >  SRR19973067.2050551/1‑150 (MQ=60)
                                                                                       AATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTT                                                                >  SRR19973067.2499878/1‑150 (MQ=60)
                                                                                        ATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTA                                                               >  SRR19973067.684758/1‑150 (MQ=60)
                                                                                        ATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTA                                                               >  SRR19973067.727285/1‑150 (MQ=60)
                                                                                         TTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTAT                                                              <  SRR19973067.2872754/150‑1 (MQ=60)
                                                                                            ATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCAT                                                           <  SRR19973067.873637/150‑1 (MQ=60)
                                                                                             TGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATC                                                          <  SRR19973067.291931/150‑1 (MQ=60)
                                                                                              GGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCG                                                         <  SRR19973067.2129922/150‑1 (MQ=60)
                                                                                              GGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCG                                                         <  SRR19973067.3284093/150‑1 (MQ=60)
                                                                                              GGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCG                                                         <  SRR19973067.2326391/150‑1 (MQ=60)
                                                                                               GCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGC                                                        >  SRR19973067.916218/1‑150 (MQ=60)
                                                                                                 AGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTC                                                      <  SRR19973067.927172/150‑1 (MQ=60)
                                                                                                 AGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTC                                                      >  SRR19973067.1702608/1‑150 (MQ=60)
                                                                                                      CTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATA                                                 >  SRR19973067.892588/1‑150 (MQ=60)
                                                                                                      CTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATA                                                 >  SRR19973067.1119549/1‑150 (MQ=60)
                                                                                                      CTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATA                                                 <  SRR19973067.1169536/150‑1 (MQ=60)
                                                                                                        CTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATG                                               >  SRR19973067.345321/1‑150 (MQ=60)
                                                                                                             TGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCAT                                          >  SRR19973067.1927543/1‑150 (MQ=60)
                                                                                                              GTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCCAATATGCCCATC                                         >  SRR19973067.1230094/1‑150 (MQ=60)
                                                                                                                  AAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGT                                     <  SRR19973067.722535/150‑1 (MQ=60)
                                                                                                                  AAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGT                                     <  SRR19973067.722530/150‑1 (MQ=60)
                                                                                                                   AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                    <  SRR19973067.3013485/150‑1 (MQ=60)
                                                                                                                   AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                    <  SRR19973067.3013483/150‑1 (MQ=60)
                                                                                                                   AAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTG                                    <  SRR19973067.3013489/150‑1 (MQ=60)
                                                                                                                      CCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGA                                 >  SRR19973067.237430/1‑150 (MQ=60)
                                                                                                                        TCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGA                               <  SRR19973067.902051/150‑1 (MQ=60)
                                                                                                                         CACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGAT                              <  SRR19973067.1896152/150‑1 (MQ=60)
                                                                                                                         CACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGAT                              <  SRR19973067.2348602/150‑1 (MQ=60)
                                                                                                                         CACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGAT                              >  SRR19973067.742476/1‑150 (MQ=60)
                                                                                                                            CCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAT                           <  SRR19973067.343819/150‑1 (MQ=60)
                                                                                                                                 AAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGC                      >  SRR19973067.3480807/1‑150 (MQ=60)
                                                                                                                                 AAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGC                      >  SRR19973067.3318483/1‑150 (MQ=60)
                                                                                                                                 AAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGC                      >  SRR19973067.3318485/1‑150 (MQ=60)
                                                                                                                                     CCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACT                  <  SRR19973067.718127/150‑1 (MQ=60)
                                                                                                                                     CCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACT                  >  SRR19973067.1203316/1‑150 (MQ=60)
                                                                                                                                     CCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACT                  >  SRR19973067.560198/1‑150 (MQ=60)
                                                                                                                                     CCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACT                  <  SRR19973067.14498/150‑1 (MQ=60)
                                                                                                                                        TGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTG               <  SRR19973067.2928765/150‑1 (MQ=60)
                                                                                                                                          CGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGG             <  SRR19973067.1227048/150‑1 (MQ=60)
                                                                                                                                          CGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGG             >  SRR19973067.3293782/1‑150 (MQ=60)
                                                                                                                                             AACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCA          >  SRR19973067.778706/1‑150 (MQ=60)
                                                                                                                                             AACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCA          >  SRR19973067.778704/1‑150 (MQ=60)
                                                                                                                                             AACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCA          >  SRR19973067.164612/1‑150 (MQ=60)
                                                                                                                                                  CAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATG     >  SRR19973067.2389733/1‑150 (MQ=60)
                                                                                                                                                   AGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGG    >  SRR19973067.2436466/1‑150 (MQ=60)
                                                                                                                                                     AAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGG  >  SRR19973067.494106/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGG  >  CP009273/1514709‑1515007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑