Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,531,029 G→T 100% R180S (CGC→AGC)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,531,0290GT95.2% 614.6 / 20.6 220R180S (CGC→AGC) narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base G (5/6);  new base T (117/92);  total (122/98)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 221 total aligned reads displayed.

CCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAT  >  CP009273/1530885‑1531173
                                                                                                                                                |                                                                                                                                                
  gCACTGATGTCGACATATTGCGGCGCGAAATCCTGGCTAACGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGgtgt                                                                                                                                             <  1:306977/148‑1 (MQ=255)
    aCTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCa                                                                                                                                           <  2:3331280/148‑1 (MQ=255)
     cTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCAt                                                                                                                                          <  2:2587718/148‑1 (MQ=255)
      tGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAATCGCCTGGCTGGTGTCATc                                                                                                                                         >  1:309316/1‑148 (MQ=255)
        aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATcgc                                                                                                                                       >  1:2326455/1‑148 (MQ=255)
        aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATcgc                                                                                                                                       >  1:640137/1‑148 (MQ=255)
             gACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctct                                                                                                                                  <  1:1685846/148‑1 (MQ=255)
             gACATAGTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctct                                                                                                                                  <  2:290230/148‑1 (MQ=255)
              aCATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCtt                                                                                                                                 <  2:1973278/148‑1 (MQ=255)
               cATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTc                                                                                                                                >  1:262660/1‑148 (MQ=255)
                  atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCt                                                                                                                             >  1:363783/1‑148 (MQ=255)
                    tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGt                                                                                                                           >  2:15206/1‑148 (MQ=255)
                    tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGt                                                                                                                           >  2:1301081/1‑148 (MQ=255)
                    tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGt                                                                                                                           <  2:1280966/148‑1 (MQ=255)
                      cggcgCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGttt                                                                                                                         <  1:1056053/148‑1 (MQ=255)
                            gACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGt                                                                                                                   <  1:1829347/148‑1 (MQ=255)
                            gACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGt                                                                                                                   <  1:1828810/148‑1 (MQ=255)
                             aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtt                                                                                                                  <  2:428648/148‑1 (MQ=255)
                             aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTGTACTTGtt                                                                                                                  >  2:2246260/1‑148 (MQ=255)
                             aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGtt                                                                                                                  <  2:1043371/148‑1 (MQ=255)
                              cATCCTGGCTAAAGCGTCGCTGATATTTATTAATCGGCGAACTGTCACACGCCGTGGCGTTAACTTCAATAAACTTCACCTTTTCCTCTTCCCACACAGCATCAAGCGCCAGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGttt                                                                                                                 >  1:752453/1‑148 (MQ=255)
                                tCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtg                                                                                                               <  1:139976/148‑1 (MQ=255)
                                tCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTCTtg                                                                                                               >  1:2198475/1‑148 (MQ=255)
                                 ccTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgt                                                                                                              >  1:517857/1‑148 (MQ=255)
                                 ccTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgt                                                                                                              <  2:3136465/148‑1 (MQ=255)
                                   tGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGa                                                                                                            <  1:2458804/148‑1 (MQ=255)
                                   tGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCACTCTTCGCTGTTTACTTGTTTTGTGa                                                                                                            >  2:2659621/1‑148 (MQ=255)
                                   tGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGa                                                                                                            <  2:1661050/148‑1 (MQ=255)
                                    ggCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGac                                                                                                           >  2:1478190/1‑148 (MQ=255)
                                    ggCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGGTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGac                                                                                                           >  1:3352617/1‑148 (MQ=255)
                                      cTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGacac                                                                                                         <  1:839086/148‑1 (MQ=255)
                                      cTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGacac                                                                                                         <  1:1577351/148‑1 (MQ=255)
                                       tAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACt                                                                                                        >  1:3156129/1‑148 (MQ=255)
                                        aaaGCGTCGCTGATATTGATTAAGCTGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                       <  2:853457/148‑1 (MQ=255)
                                        aaaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                       <  2:3387416/148‑1 (MQ=255)
                                         aaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGt                                                                                                      >  2:548137/1‑148 (MQ=255)
                                         aaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGt                                                                                                      >  1:3515164/1‑148 (MQ=255)
                                         aaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCCTGGCGTTATCTTCAATAAACTTCACCAGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGACACTGt                                                                                                      >  1:653385/1‑148 (MQ=255)
                                          aGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTc                                                                                                     >  1:2623910/1‑148 (MQ=255)
                                             gTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACt                                                                                                  <  1:15097/148‑1 (MQ=255)
                                             gTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACt                                                                                                  <  1:1301084/148‑1 (MQ=255)
                                             gTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACt                                                                                                  <  1:2507061/148‑1 (MQ=255)
                                                   gATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAg                                                                                            >  2:1908112/1‑148 (MQ=255)
                                                   gATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAg                                                                                            <  2:2872685/148‑1 (MQ=255)
                                                    atatTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAgg                                                                                           <  1:2772239/148‑1 (MQ=255)
                                                    atatTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAgg                                                                                           <  1:2328529/148‑1 (MQ=255)
                                                     tatTGATTAAGCGGCGAGCTGTCACACGCCGTTGCGTTATCTTCAATAAACTTCAACTTTTCCTCTTCCCACACTGCATAAAGCGCCTGTCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAggg                                                                                          <  1:3317141/148‑1 (MQ=255)
                                                      atTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGa                                                                                         >  2:2174743/1‑148 (MQ=255)
                                                        tGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGc                                                                                       >  1:3385587/1‑148 (MQ=255)
                                                           ttAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTc                                                                                    <  1:163438/148‑1 (MQ=255)
                                                            tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                   >  2:935022/1‑148 (MQ=255)
                                                            tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                   <  1:19731/148‑1 (MQ=255)
                                                            tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                   >  2:1691799/1‑148 (MQ=255)
                                                            tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                   >  2:1940340/1‑148 (MQ=255)
                                                                     aGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTg                                                                          >  2:993604/1‑148 (MQ=255)
                                                                      gCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgc                                                                         >  2:1728890/1‑148 (MQ=255)
                                                                       cTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgca                                                                        >  2:2059821/1‑148 (MQ=255)
                                                                       cTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgca                                                                        >  1:1192727/1‑148 (MQ=255)
                                                                         gTCACACGCCGTGGCGTTATCTTCAATAAACTTCCCCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagc                                                                      <  2:1128446/148‑1 (MQ=255)
                                                                         gTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagc                                                                      >  2:277313/1‑148 (MQ=255)
                                                                          tCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca                                                                     <  2:3099289/148‑1 (MQ=255)
                                                                           cacaCGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGcagcag                                                                    <  1:1255109/148‑1 (MQ=255)
                                                                              acGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGc                                                                 <  1:2872561/148‑1 (MQ=255)
                                                                                      gCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaaca                                                         <  2:2326455/148‑1 (MQ=255)
                                                                                      gCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaaca                                                         >  1:102888/1‑148 (MQ=255)
                                                                                           aTCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAcgcg                                                    <  2:3540845/148‑1 (MQ=255)
                                                                                            tCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGt                                                   >  1:2922866/1‑148 (MQ=255)
                                                                                             cTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTa                                                  >  1:1105045/1‑148 (MQ=255)
                                                                                             cTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTa                                                  <  1:3388398/148‑1 (MQ=255)
                                                                                                cAATAAACTTCAACTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGAAGCAGAGCATCAAACAACGAGTACCa                                               >  2:2441823/1‑148 (MQ=255)
                                                                                                   tAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACgg                                            <  1:2962598/148‑1 (MQ=255)
                                                                                                      aCTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGc                                         >  1:1769089/1‑148 (MQ=255)
                                                                                                        ttCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCt                                       >  2:2087293/1‑148 (MQ=255)
                                                                                                        ttCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCt                                       >  2:2336870/1‑148 (MQ=255)
                                                                                                         tCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTc                                      >  2:1776214/1‑148 (MQ=255)
                                                                                                         tCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTc                                      >  2:3537506/1‑148 (MQ=255)
                                                                                                            ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcgcg                                   >  1:431691/1‑148 (MQ=255)
                                                                                                             cTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                  >  1:1739024/1‑148 (MQ=255)
                                                                                                               gTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttg                                <  1:1478190/148‑1 (MQ=255)
                                                                                                               gTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttg                                >  1:142562/1‑148 (MQ=255)
                                                                                                               gTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttg                                >  2:2427005/1‑148 (MQ=255)
                                                                                                                 tCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgtt                              <  1:1924017/148‑1 (MQ=255)
                                                                                                                 tCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgtt                              >  2:1848055/1‑148 (MQ=255)
                                                                                                                  ccTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                             >  2:1178084/1‑148 (MQ=255)
                                                                                                                   ctctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGtttt                            >  2:1740717/1‑148 (MQ=255)
                                                                                                                   ctctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGtttt                            >  2:3505330/1‑148 (MQ=255)
                                                                                                                    tctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTa                           >  2:1589179/1‑148 (MQ=255)
                                                                                                                     cgtCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTaa                          <  1:1183892/146‑1 (MQ=255)
                                                                                                                       tCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGc                        >  2:1608957/1‑148 (MQ=255)
                                                                                                                            cacTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCg                   >  2:3042956/1‑148 (MQ=255)
                                                                                                                             acTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGc                  >  2:1165439/1‑148 (MQ=255)
                                                                                                                             acTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGc                  >  1:1573402/1‑148 (MQ=255)
                                                                                                                              cTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGcc                 <  1:1795702/148‑1 (MQ=255)
                                                                                                                               tGCATCACGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTGGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCa                <  2:441832/148‑1 (MQ=255)
                                                                                                                                 cATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAg              <  2:2089165/148‑1 (MQ=255)
                                                                                                                                   tCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCa            >  1:1802913/1‑148 (MQ=255)
                                                                                                                                      aGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg         >  1:1268448/1‑148 (MQ=255)
                                                                                                                                            tGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa   >  2:616066/1‑148 (MQ=255)
                                                                                                                                             ggCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAt  <  2:2469092/148‑1 (MQ=255)
                                                                                                                                             ggCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGGGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAt  <  2:262660/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAT  >  CP009273/1530885‑1531173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCG  >  CP009273/1530883‑1531178
                                                                                                                                                  |                                                                                                                                                     
TCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGT                                                                                                                                                    <  SRR19973066.2059809/150‑1 (MQ=60)
 CCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTG                                                                                                                                                   >  SRR19973066.937585/1‑150 (MQ=60)
  CCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGT                                                                                                                                                  >  SRR19973066.2485929/1‑150 (MQ=60)
  CCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGT                                                                                                                                                  <  SRR19973066.2344116/150‑1 (MQ=60)
    GCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCA                                                                                                                                                <  SRR19973066.839931/150‑1 (MQ=60)
    GCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCA                                                                                                                                                >  SRR19973066.2904240/1‑150 (MQ=60)
    GCACTGATGTCGACATATTGCGGCGCGAAATCCTGGCTAACGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCA                                                                                                                                                <  SRR19973066.309195/150‑1 (MQ=60)
     CACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCANACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCAT                                                                                                                                               <  SRR19973066.1820330/150‑1 (MQ=60)
      ACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAATCGCCTGGCTGGTGTCATC                                                                                                                                              >  SRR19973066.311575/1‑150 (MQ=60)
      ACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATC                                                                                                                                              >  SRR19973066.3410021/1‑150 (MQ=60)
        TGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGC                                                                                                                                            >  SRR19973066.2342338/1‑150 (MQ=60)
        TGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGC                                                                                                                                            >  SRR19973066.2447550/1‑150 (MQ=60)
        TGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTACCACACTGCATCAAGCGCCTGGCTGGTGTCATCGC                                                                                                                                            >  SRR19973066.644762/1‑150 (MQ=60)
           TGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACATCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCT                                                                                                                                         >  SRR19973066.339595/1‑150 (MQ=60)
            GTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTC                                                                                                                                        >  SRR19973066.510780/1‑150 (MQ=60)
               GACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTC                                                                                                                                     >  SRR19973066.264631/1‑150 (MQ=60)
               GACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTC                                                                                                                                     <  SRR19973066.3332456/150‑1 (MQ=60)
               GACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTC                                                                                                                                     <  SRR19973066.1698122/150‑1 (MQ=60)
                  ATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCT                                                                                                                                  >  SRR19973066.366639/1‑150 (MQ=60)
                  ATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCT                                                                                                                                  >  SRR19973066.2956476/1‑150 (MQ=60)
                     TTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTT                                                                                                                               <  SRR19973066.529034/150‑1 (MQ=60)
                        CGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTAC                                                                                                                            <  SRR19973066.1063929/150‑1 (MQ=60)
                        CGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTAC                                                                                                                            <  SRR19973066.1063814/150‑1 (MQ=60)
                         GGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACT                                                                                                                           <  SRR19973066.1936007/150‑1 (MQ=60)
                              GACATCCTGGCTAAAGCGTCGCTGATATTTATTAATCGGCGAACTGTCACACGCCGTGGCGTTAACTTCAATAAACTTCACCTTTTCCTCTTCCCACACAGCATCAAGCGCCAGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTT                                                                                                                      >  SRR19973066.758034/1‑150 (MQ=60)
                              GACATCCTGGCTAAAGCGTCGCTGATATTGATTAATCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAANTTCACCTGTTCCTCTTACCACACTGCATCAAGCGCCTGGCTGGTGTCATAGCGCTCTTCGGTGTTTACTTGTTT                                                                                                                      >  SRR19973066.1820721/1‑150 (MQ=60)
                              GACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTT                                                                                                                      <  SRR19973066.1841868/150‑1 (MQ=60)
                              GACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTT                                                                                                                      <  SRR19973066.1842136/150‑1 (MQ=60)
                              GACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTT                                                                                                                      <  SRR19973066.1842407/150‑1 (MQ=60)
                                CATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTCTTG                                                                                                                    >  SRR19973066.2213717/1‑150 (MQ=60)
                                CATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTAACCTGTTCCTCGTCCCACACTGCAACAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTAATTGTCTTG                                                                                                                    >  SRR19973066.407133/1‑150 (MQ=60)
                                 ATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGT                                                                                                                   >  SRR19973066.1813838/1‑150 (MQ=60)
                                 ATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGT                                                                                                                   <  SRR19973066.2542811/150‑1 (MQ=60)
                                 ATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGT                                                                                                                   >  SRR19973066.521671/1‑150 (MQ=60)
                                  TCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTG                                                                                                                  <  SRR19973066.141327/150‑1 (MQ=60)
                                  TCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTG                                                                                                                  <  SRR19973066.2642250/150‑1 (MQ=60)
                                    CTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGAC                                                                                                                >  SRR19973066.3373978/1‑150 (MQ=60)
                                    CTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGGTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGAC                                                                                                                >  SRR19973066.3374960/1‑150 (MQ=60)
                                     TGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACA                                                                                                               <  SRR19973066.2475565/150‑1 (MQ=60)
                                     TGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGAGACA                                                                                                               >  SRR19973066.170612/1‑150 (MQ=60)
                                       GCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACT                                                                                                             >  SRR19973066.3177166/1‑150 (MQ=60)
                                        CTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTG                                                                                                            <  SRR19973066.845194/150‑1 (MQ=60)
                                        CTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTG                                                                                                            >  SRR19973066.2583399/1‑150 (MQ=60)
                                        CTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTG                                                                                                            <  SRR19973066.1588730/150‑1 (MQ=60)
                                        CTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTG                                                                                                            >  SRR19973066.1689898/1‑150 (MQ=60)
                                        CTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTG                                                                                                            >  SRR19973066.1689887/1‑150 (MQ=60)
                                         TAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGT                                                                                                           >  SRR19973066.3538574/1‑150 (MQ=60)
                                         TAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCCTGGCGTTATCTTCAATAAACTTCACCAGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGACACTGT                                                                                                           >  SRR19973066.658153/1‑150 (MQ=60)
                                          AAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTC                                                                                                          >  SRR19973066.2641605/1‑150 (MQ=60)
                                              CGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTT                                                                                                      <  SRR19973066.2318298/150‑1 (MQ=60)
                                               GTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTG                                                                                                     <  SRR19973066.1310511/150‑1 (MQ=60)
                                               GTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTG                                                                                                     <  SRR19973066.1310514/150‑1 (MQ=60)
                                               GTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTG                                                                                                     <  SRR19973066.2524134/150‑1 (MQ=60)
                                               GTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTG                                                                                                     <  SRR19973066.15439/150‑1 (MQ=60)
                                               GTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTG                                                                                                     <  SRR19973066.15330/150‑1 (MQ=60)
                                                 CGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAA                                                                                                   <  SRR19973066.3482776/150‑1 (MQ=60)
                                                    TGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGG                                                                                                <  SRR19973066.3350145/150‑1 (MQ=60)
                                                    TGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGG                                                                                                >  SRR19973066.1500476/1‑150 (MQ=60)
                                                    TGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGG                                                                                                >  SRR19973066.1390190/1‑150 (MQ=60)
                                                     GATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGG                                                                                               >  SRR19973066.1800560/1‑150 (MQ=60)
                                                      ATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGA                                                                                              <  SRR19973066.2790777/150‑1 (MQ=60)
                                                      ATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGA                                                                                              <  SRR19973066.2344425/150‑1 (MQ=60)
                                                      ATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGA                                                                                              <  SRR19973066.2790906/150‑1 (MQ=60)
                                                       TATTGATTAAGCGGCGAGCTGTCACACGCCGTTGCGTTATCTTCAATAAACTTCAACTTTTCCTCTTCCCACACTGCATAAAGCGCCTGTCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAG                                                                                             <  SRR19973066.3339163/150‑1 (MQ=60)
                                                        ATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGC                                                                                            >  SRR19973066.3408179/1‑150 (MQ=60)
                                                           GATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTC                                                                                         >  SRR19973066.1602001/1‑150 (MQ=60)
                                                            ATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCC                                                                                        >  SRR19973066.1952091/1‑150 (MQ=60)
                                                             TTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCC                                                                                       <  SRR19973066.1267189/150‑1 (MQ=60)
                                                             TTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCC                                                                                       <  SRR19973066.164920/150‑1 (MQ=60)
                                                              TAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCG                                                                                      <  SRR19973066.20028/150‑1 (MQ=60)
                                                                 GCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCA                                                                                   <  SRR19973066.2125851/150‑1 (MQ=60)
                                                                   GGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGT                                                                                 >  SRR19973066.116802/1‑150 (MQ=60)
                                                                      GAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGC                                                                              >  SRR19973066.1483807/1‑150 (MQ=60)
                                                                       AGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCA                                                                             >  SRR19973066.1201041/1‑150 (MQ=60)
                                                                       AGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCA                                                                             >  SRR19973066.1201071/1‑150 (MQ=60)
                                                                          TGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCA                                                                          <  SRR19973066.3113660/150‑1 (MQ=60)
                                                                             CACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAG                                                                       <  SRR19973066.1264145/150‑1 (MQ=60)
                                                                             CACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAG                                                                       <  SRR19973066.1264121/150‑1 (MQ=60)
                                                                                ACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCAT                                                                    <  SRR19973066.2891825/150‑1 (MQ=60)
                                                                                ACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCAT                                                                    <  SRR19973066.684968/150‑1 (MQ=60)
                                                                                ACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCAT                                                                    <  SRR19973066.685011/150‑1 (MQ=60)
                                                                                      TGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACA                                                              >  SRR19973066.103975/1‑150 (MQ=60)
                                                                                           TTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCG                                                         >  SRR19973066.1253158/1‑150 (MQ=60)
                                                                                            TATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGT                                                        >  SRR19973066.2942346/1‑150 (MQ=60)
                                                                                             ATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTA                                                       >  SRR19973066.1113052/1‑150 (MQ=60)
                                                                                             ATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTA                                                       >  SRR19973066.3073645/1‑150 (MQ=60)
                                                                                               CTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACC                                                     <  SRR19973066.3411010/150‑1 (MQ=60)
                                                                                                   AATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGG                                                 >  SRR19973066.1414822/1‑150 (MQ=60)
                                                                                                     TAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTG                                               <  SRR19973066.2982211/150‑1 (MQ=60)
                                                                                                      AAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGC                                              >  SRR19973066.1781764/1‑150 (MQ=60)
                                                                                                        ACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCT                                            <  SRR19973066.1461317/150‑1 (MQ=60)
                                                                                                            CACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTTTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCG                                        >  SRR19973066.65327/1‑150 (MQ=60)
                                                                                                            CACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCG                                        >  SRR19973066.435036/1‑150 (MQ=60)
                                                                                                             ACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGT                                       >  SRR19973066.1751573/1‑150 (MQ=60)
                                                                                                               CTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTG                                     >  SRR19973066.143935/1‑150 (MQ=60)
                                                                                                                 GTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTT                                   <  SRR19973066.1488590/150‑1 (MQ=60)
                                                                                                                   TCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTT                                 <  SRR19973066.3156036/150‑1 (MQ=60)
                                                                                                                   TCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTT                                 <  SRR19973066.1937698/150‑1 (MQ=60)
                                                                                                                   TCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTT                                 <  SRR19973066.2000398/150‑1 (MQ=60)
                                                                                                                       CTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGC                             >  SRR19973066.3552567/1‑150 (MQ=60)
                                                                                                                       CTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGC                             >  SRR19973066.3552467/1‑150 (MQ=60)
                                                                                                                       CGTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGC                             <  SRR19973066.1192204/150‑1 (MQ=60)
                                                                                                                             ACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGC                       >  SRR19973066.1585155/1‑150 (MQ=60)
                                                                                                                             ACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGC                       >  SRR19973066.1584743/1‑150 (MQ=60)
                                                                                                                              CACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCC                      >  SRR19973066.1063066/1‑150 (MQ=60)
                                                                                                                                CTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAA                    <  SRR19973066.1808476/150‑1 (MQ=60)
                                                                                                                                   CATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCA                 >  SRR19973066.1815719/1‑150 (MQ=60)
                                                                                                                                   CATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCA                 >  SRR19973066.2949243/1‑150 (MQ=60)
                                                                                                                                      CAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGG              >  SRR19973066.1277606/1‑150 (MQ=60)
                                                                                                                                           GCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGG         <  SRR19973066.1778898/150‑1 (MQ=60)
                                                                                                                                              TGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATC      >  SRR19973066.1078734/1‑150 (MQ=60)
                                                                                                                                               GGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCG     <  SRR19973066.279338/150‑1 (MQ=60)
                                                                                                                                                GCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGG    <  SRR19973066.3241328/150‑1 (MQ=60)
                                                                                                                                                  TGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCG  >  SRR19973066.389085/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
TCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCG  >  CP009273/1530883‑1531178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑