Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,531,029 G→T 100% R180S (CGC→AGC)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,531,0290GT96.3% 533.7 / 7.5 188R180S (CGC→AGC) narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base G (6/3);  new base T (93/86);  total (99/89)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 194 total aligned reads displayed.

CCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGG  >  CP009273/1530884‑1531176
                                                                                                                                                 |                                                                                                                                                   
cccGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCctggctgg                                                                                                                                                   <  2:1218165/148‑1 (MQ=255)
   gCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGgtgt                                                                                                                                                <  1:799732/148‑1 (MQ=255)
      cTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCAt                                                                                                                                             >  1:2293332/1‑148 (MQ=255)
      cTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACAGCATCAAGCGCCTGGCTGGTGTCAt                                                                                                                                             >  1:720746/1‑148 (MQ=255)
      cTGATGTCGACATATGGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCAt                                                                                                                                             <  1:958963/148‑1 (MQ=255)
        gATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATcg                                                                                                                                           >  1:3408934/1‑148 (MQ=255)
         aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATcgc                                                                                                                                          >  1:370985/1‑148 (MQ=255)
              gACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctct                                                                                                                                     <  2:2672021/148‑1 (MQ=255)
               aCATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCtt                                                                                                                                    <  2:22803/148‑1 (MQ=255)
                cATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTc                                                                                                                                   >  2:2211855/1‑148 (MQ=255)
                cATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTc                                                                                                                                   >  2:287291/1‑148 (MQ=255)
                cATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTc                                                                                                                                   >  2:1992188/1‑148 (MQ=255)
                cATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTc                                                                                                                                   >  2:2788284/1‑148 (MQ=255)
                cATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTc                                                                                                                                   >  2:336009/1‑148 (MQ=255)
                 attttGCGGCGCTACTGCGTGCCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCg                                                                                                                                  <  1:3238639/145‑1 (MQ=255)
                 atatTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCg                                                                                                                                  <  1:1960871/148‑1 (MQ=255)
                   atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCt                                                                                                                                >  2:2753129/1‑148 (MQ=255)
                   atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCt                                                                                                                                >  1:428214/1‑148 (MQ=255)
                   atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCt                                                                                                                                >  2:552982/1‑148 (MQ=255)
                     tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCGGt                                                                                                                              >  1:3411693/1‑148 (MQ=255)
                     tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGt                                                                                                                              >  2:2677340/1‑148 (MQ=255)
                     tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATATTCAATAAAATTCACCTGTTCCTCTTCCCACACAGAATCAAGAGCGTGGCGGGTGTCCTCGCGCTCTTCGCTGt                                                                                                                              >  2:590266/1‑148 (MQ=255)
                           gcgACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCGTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACtt                                                                                                                        <  1:3421902/148‑1 (MQ=255)
                            cgACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTg                                                                                                                       <  1:644105/148‑1 (MQ=255)
                              aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtt                                                                                                                     <  2:1595872/148‑1 (MQ=255)
                              aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGGTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtt                                                                                                                     <  2:2090535/148‑1 (MQ=255)
                                  ccTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgt                                                                                                                 >  1:2760510/1‑148 (MQ=255)
                                  ccTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgt                                                                                                                 >  2:1079123/1‑148 (MQ=255)
                                   cTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgtg                                                                                                                <  1:1527718/148‑1 (MQ=255)
                                    tGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGa                                                                                                               <  1:1339391/148‑1 (MQ=255)
                                     ggCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGac                                                                                                              >  2:735613/1‑148 (MQ=255)
                                       cTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGacac                                                                                                            <  1:1445779/148‑1 (MQ=255)
                                       cTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCGTCGCTGTTTACTTGTTTTGTGacac                                                                                                            >  1:1702693/1‑148 (MQ=255)
                                        tataGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATTAACTTCAGCTGTTCCTATTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACt                                                                                                           <  2:2353063/145‑1 (MQ=255)
                                         aaaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                          <  1:2443609/148‑1 (MQ=255)
                                            gCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTACATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCa                                                                                                       <  2:1797574/148‑1 (MQ=255)
                                            gCGTCGCTGATATTGAGTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTACATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCa                                                                                                       <  2:1796983/148‑1 (MQ=255)
                                               tCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACtt                                                                                                    >  1:429482/1‑148 (MQ=255)
                                               tCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCCGTTTACTTGTTTTGTGACACTGTCACtt                                                                                                    >  1:429714/1‑148 (MQ=255)
                                                cGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTg                                                                                                   >  1:1297081/1‑148 (MQ=255)
                                                cGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTg                                                                                                   >  1:2884606/1‑148 (MQ=255)
                                                  cTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaa                                                                                                 >  2:438508/1‑148 (MQ=255)
                                                  cTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaa                                                                                                 >  1:2775052/1‑148 (MQ=255)
                                                   tGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaaa                                                                                                <  2:2132918/148‑1 (MQ=255)
                                                   tGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaaa                                                                                                <  2:1937856/148‑1 (MQ=255)
                                                   tGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaaa                                                                                                <  2:163120/148‑1 (MQ=255)
                                                   tGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCGTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaaa                                                                                                <  2:240829/148‑1 (MQ=255)
                                                      tatTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAggg                                                                                             <  2:457490/148‑1 (MQ=255)
                                                       atTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGa                                                                                            >  2:3131647/1‑148 (MQ=255)
                                                        ttGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAg                                                                                           >  2:1731078/1‑148 (MQ=255)
                                                             tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                      >  2:2856570/1‑148 (MQ=255)
                                                             tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                      <  1:2017198/148‑1 (MQ=255)
                                                                gcggcgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGc                                                                                   <  1:3144959/148‑1 (MQ=255)
                                                                gcggcgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGc                                                                                   <  2:2992871/148‑1 (MQ=255)
                                                                  ggcgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCa                                                                                 <  2:252610/148‑1 (MQ=255)
                                                                      aGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTg                                                                             <  1:2610129/148‑1 (MQ=255)
                                                                      aGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTg                                                                             <  1:1816725/148‑1 (MQ=255)
                                                                         tGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcag                                                                          <  1:3061627/148‑1 (MQ=255)
                                                                          gTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagc                                                                         <  1:2768687/148‑1 (MQ=255)
                                                                                cGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCa                                                                   >  1:1038742/1‑148 (MQ=255)
                                                                                        cGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaacaa                                                           <  2:1881756/148‑1 (MQ=255)
                                                                                        cGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaacaa                                                           <  2:1516220/148‑1 (MQ=255)
                                                                                        cGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaacaa                                                           <  1:2208153/148‑1 (MQ=255)
                                                                                           tATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAcgc                                                        >  1:1754515/1‑148 (MQ=255)
                                                                                              cTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTa                                                     >  2:771343/1‑148 (MQ=255)
                                                                                              cTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTa                                                     >  2:1307431/1‑148 (MQ=255)
                                                                                              cTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTa                                                     <  1:2151248/148‑1 (MQ=255)
                                                                                                tCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTAcc                                                   >  1:713024/1‑148 (MQ=255)
                                                                                                tCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTAcc                                                   >  2:1298293/1‑148 (MQ=255)
                                                                                                 cAATAAACTTCACCTGTTCCTCGTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCa                                                  <  2:3440742/148‑1 (MQ=255)
                                                                                                   aTAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACg                                                <  1:1912369/148‑1 (MQ=255)
                                                                                                         ttCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCt                                          >  2:508957/1‑148 (MQ=255)
                                                                                                             ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcgcg                                      >  1:286422/1‑148 (MQ=255)
                                                                                                              cTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                     >  1:277884/1‑148 (MQ=255)
                                                                                                                gTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCGCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttg                                   >  2:1012727/1‑148 (MQ=255)
                                                                                                                  tCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgtt                                 >  1:522182/1‑148 (MQ=255)
                                                                                                                  tCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgtt                                 >  1:2522796/1‑148 (MQ=255)
                                                                                                                   ccTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                                >  2:281571/1‑148 (MQ=255)
                                                                                                                   ccTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                                >  2:158994/1‑148 (MQ=255)
                                                                                                                     tctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTa                              >  1:1356236/1‑148 (MQ=255)
                                                                                                                      ctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTaa                             <  2:1385097/148‑1 (MQ=255)
                                                                                                                      cgtCCCACCCTGCCACAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTaa                             <  2:3131506/146‑1 (MQ=255)
                                                                                                                         cccACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCg                          <  1:1004394/148‑1 (MQ=255)
                                                                                                                          ccACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                         <  2:1027577/148‑1 (MQ=255)
                                                                                                                          ccACACTGCATCAAGCGCCTGGCTGGTGTCATCGCACTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                         >  1:2095592/1‑148 (MQ=255)
                                                                                                                           cacaCTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAc                        >  2:3154680/1‑148 (MQ=255)
                                                                                                                              acTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGc                     <  2:70459/148‑1 (MQ=255)
                                                                                                                               cTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGcc                    <  2:1712172/148‑1 (MQ=255)
                                                                                                                                  cATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAg                 <  1:3167707/148‑1 (MQ=255)
                                                                                                                                  cATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAg                 >  2:126237/1‑148 (MQ=255)
                                                                                                                                  cATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAg                 <  1:552982/148‑1 (MQ=255)
                                                                                                                                    tCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCa               >  2:2314410/1‑148 (MQ=255)
                                                                                                                                     cAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAg              >  1:967181/1‑148 (MQ=255)
                                                                                                                                     cAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAg              >  1:540790/1‑148 (MQ=255)
                                                                                                                                       aGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg            >  1:988773/1‑148 (MQ=255)
                                                                                                                                       aGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg            >  1:1356005/1‑148 (MQ=255)
                                                                                                                                        gcgcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGAGACCGCCAAGCAggcc           >  1:2677619/1‑146 (MQ=255)
                                                                                                                                         cgcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCg          <  1:295144/148‑1 (MQ=255)
                                                                                                                                             tGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa      >  1:3014760/1‑148 (MQ=255)
                                                                                                                                                 gggTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCgg  <  1:1126315/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGG  >  CP009273/1530884‑1531176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCG  >  CP009273/1530883‑1531178
                                                                                                                                                  |                                                                                                                                                     
TCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGT                                                                                                                                                    <  SRR19973065.2611030/150‑1 (MQ=60)
    GCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCA                                                                                                                                                <  SRR19973065.804906/150‑1 (MQ=60)
    GCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCA                                                                                                                                                <  SRR19973065.805017/150‑1 (MQ=60)
     CACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCAT                                                                                                                                               >  SRR19973065.2307782/1‑150 (MQ=60)
     CACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACAGCATCAAGCGCCTGGCTGGTGTCAT                                                                                                                                               >  SRR19973065.725568/1‑150 (MQ=60)
     CACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTAAATAAACTTAACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCAT                                                                                                                                               >  SRR19973065.725542/1‑150 (MQ=60)
      ACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATC                                                                                                                                              <  SRR19973065.3461073/150‑1 (MQ=60)
       CTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCG                                                                                                                                             >  SRR19973065.3429764/1‑150 (MQ=60)
       CTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCG                                                                                                                                             >  SRR19973065.2747322/1‑150 (MQ=60)
       CTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCG                                                                                                                                             <  SRR19973065.965359/150‑1 (MQ=60)
       CTGATGTCGACATATGGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCG                                                                                                                                             <  SRR19973065.965315/150‑1 (MQ=60)
        TGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATAGC                                                                                                                                            >  SRR19973065.373582/1‑150 (MQ=60)
          ATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGC                                                                                                                                          >  SRR19973065.459603/1‑150 (MQ=60)
              CGACATCTTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCAGCAAGCGCCTGGCTGGTGTCATCGCGCTCTT                                                                                                                                      <  SRR19973065.2605263/150‑1 (MQ=60)
                  attttgcggcgctactgcgtgcCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCT                                                                                                                                  <  SRR19973065.3258142/128‑1 (MQ=60)
                  ATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAATCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTAACCTGTTCCTCTTACCACACTGCATCAATCGCCTGGCTGGTGTCATAGCGCTCTTCGCT                                                                                                                                  >  SRR19973065.1823464/1‑150 (MQ=60)
                  ATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCT                                                                                                                                  >  SRR19973065.431188/1‑150 (MQ=60)
                  ATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCT                                                                                                                                  <  SRR19973065.1973676/150‑1 (MQ=60)
                   TATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTG                                                                                                                                 >  SRR19973065.71197/1‑150 (MQ=60)
                    ATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCGGT                                                                                                                                >  SRR19973065.3432538/1‑150 (MQ=60)
                           CGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTG                                                                                                                         <  SRR19973065.2274381/150‑1 (MQ=60)
                            GCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCGTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGT                                                                                                                        <  SRR19973065.3442801/150‑1 (MQ=60)
                             CGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTT                                                                                                                       <  SRR19973065.647877/150‑1 (MQ=60)
                             CGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTT                                                                                                                       <  SRR19973065.648331/150‑1 (MQ=60)
                                 ATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGT                                                                                                                   >  SRR19973065.2780402/1‑150 (MQ=60)
                                 ATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGT                                                                                                                   >  SRR19973065.2777671/1‑150 (MQ=60)
                                    CTGGCTAAAGCGTCGCTGATATTGTTTAAGCGGCGAGCTGTCACACGCCGTTGCGTTATCTTCAATAAACTTCACCTGTTACTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGAC                                                                                                                <  SRR19973065.3430068/150‑1 (MQ=60)
                                    CTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGAC                                                                                                                <  SRR19973065.1537763/150‑1 (MQ=60)
                                    CTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGAC                                                                                                                >  SRR19973065.242651/1‑150 (MQ=60)
                                     TGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACA                                                                                                               <  SRR19973065.1348256/150‑1 (MQ=60)
                                      GGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCGTCGCTGTTTACTTGTTTTGTGACAC                                                                                                              >  SRR19973065.1714039/1‑150 (MQ=60)
                                        CTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTG                                                                                                            <  SRR19973065.1455128/150‑1 (MQ=60)
                                          AAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTC                                                                                                          <  SRR19973065.2459036/150‑1 (MQ=60)
                                          AAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTC                                                                                                          <  SRR19973065.2459009/150‑1 (MQ=60)
                                           AAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCA                                                                                                         <  SRR19973065.1326940/150‑1 (MQ=60)
                                           AAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAAATTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCGGTTTACTTGTTTTGTGACACTGTCA                                                                                                         >  SRR19973065.2115039/1‑150 (MQ=60)
                                              CGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTT                                                                                                      >  SRR19973065.432463/1‑150 (MQ=60)
                                              CGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTT                                                                                                      >  SRR19973065.432531/1‑150 (MQ=60)
                                              CGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCCGTTTACTTGTTTTGTGACACTGTCACTT                                                                                                      >  SRR19973065.432696/1‑150 (MQ=60)
                                               GTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTG                                                                                                     >  SRR19973065.1305626/1‑150 (MQ=60)
                                               GTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTG                                                                                                     >  SRR19973065.2902249/1‑150 (MQ=60)
                                                 CGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAA                                                                                                   >  SRR19973065.2792281/1‑150 (MQ=60)
                                                    TGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGG                                                                                                <  SRR19973065.421496/150‑1 (MQ=60)
                                                              TAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCG                                                                                      <  SRR19973065.2030191/150‑1 (MQ=60)
                                                              TAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCG                                                                                      <  SRR19973065.2030408/150‑1 (MQ=60)
                                                                AGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCC                                                                                    >  SRR19973065.1959472/1‑150 (MQ=60)
                                                                AGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTACCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCC                                                                                    >  SRR19973065.1048122/1‑150 (MQ=60)
                                                                 GCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCA                                                                                   <  SRR19973065.3164063/150‑1 (MQ=60)
                                                                       AGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCA                                                                             <  SRR19973065.1828690/150‑1 (MQ=60)
                                                                       AGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCA                                                                             <  SRR19973065.2626312/150‑1 (MQ=60)
                                                                        GCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAG                                                                            <  SRR19973065.956613/150‑1 (MQ=60)
                                                                          TGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCA                                                                          <  SRR19973065.432377/150‑1 (MQ=60)
                                                                          TGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCA                                                                          <  SRR19973065.3080346/150‑1 (MQ=60)
                                                                          TGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCA                                                                          <  SRR19973065.1506417/150‑1 (MQ=60)
                                                                           GTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAG                                                                         <  SRR19973065.2785919/150‑1 (MQ=60)
                                                                           GTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAG                                                                         <  SRR19973065.2785888/150‑1 (MQ=60)
                                                                               CACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCA                                                                     >  SRR19973065.1045620/1‑150 (MQ=60)
                                                                                         CGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACG                                                           <  SRR19973065.1761869/150‑1 (MQ=60)
                                                                                         CGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACG                                                           <  SRR19973065.2222275/150‑1 (MQ=60)
                                                                                          GTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGC                                                          >  SRR19973065.1766058/1‑150 (MQ=60)
                                                                                               CTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACC                                                     <  SRR19973065.2165079/150‑1 (MQ=60)
                                                                                               CTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACC                                                     >  SRR19973065.717796/1‑150 (MQ=60)
                                                                                                    ATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGT                                                <  SRR19973065.1924926/150‑1 (MQ=60)
                                                                                                       AACTTCACCTGTTCCTCTTCCCACACTGCATCAACCGCCTGGCTGGTGTCATCGCTCTCTTCGCTGTTTACTTGTTTTGTGAAACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAACCACTGTGCC                                             >  SRR19973065.2994231/1‑150 (MQ=60)
                                                                                                         CTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTC                                           <  SRR19973065.289175/150‑1 (MQ=60)
                                                                                                         CTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTC                                           <  SRR19973065.2005107/150‑1 (MQ=60)
                                                                                                          TTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCG                                          >  SRR19973065.1442158/1‑150 (MQ=60)
                                                                                                            CACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCG                                        >  SRR19973065.288304/1‑150 (MQ=60)
                                                                                                             ACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGT                                       >  SRR19973065.279738/1‑150 (MQ=60)
                                                                                                             ACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGT                                       >  SRR19973065.279726/1‑150 (MQ=60)
                                                                                                               CTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTG                                     >  SRR19973065.2856923/1‑150 (MQ=60)
                                                                                                                 GTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTT                                   >  SRR19973065.2538636/1‑150 (MQ=60)
                                                                                                                 GTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTT                                   >  SRR19973065.525620/1‑150 (MQ=60)
                                                                                                                    CCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTA                                >  SRR19973065.1365224/1‑150 (MQ=60)
                                                                                                                    CCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTA                                >  SRR19973065.1365219/1‑150 (MQ=60)
                                                                                                                    CCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTA                                <  SRR19973065.1903621/150‑1 (MQ=60)
                                                                                                                     CTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAA                               >  SRR19973065.3304845/1‑150 (MQ=60)
                                                                                                                      TCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAG                              >  SRR19973065.1274905/1‑150 (MQ=60)
                                                                                                                         TCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCACTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGA                           >  SRR19973065.2109060/1‑150 (MQ=60)
                                                                                                                          CCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAC                          <  SRR19973065.1011063/150‑1 (MQ=60)
                                                                                                                                 TGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAG                   >  SRR19973065.3157035/1‑150 (MQ=60)
                                                                                                                                   CATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTTTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCA                 <  SRR19973065.3186678/150‑1 (MQ=60)
                                                                                                                                   CATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCA                 <  SRR19973065.556528/150‑1 (MQ=60)
                                                                                                                                   CATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCA                 <  SRR19973065.3186889/150‑1 (MQ=60)
                                                                                                                                   CAGCAAGCGCGTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCA                 <  SRR19973065.556116/150‑1 (MQ=60)
                                                                                                                                    ATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAG                >  SRR19973065.543917/1‑150 (MQ=60)
                                                                                                                                    ATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAG                >  SRR19973065.544300/1‑150 (MQ=60)
                                                                                                                                    ATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAG                >  SRR19973065.973599/1‑150 (MQ=60)
                                                                                                                                      CAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGG              >  SRR19973065.1364992/1‑150 (MQ=60)
                                                                                                                                      CAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGG              >  SRR19973065.1364744/1‑150 (MQ=60)
                                                                                                                                      CAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGG              >  SRR19973065.1634218/1‑150 (MQ=60)
                                                                                                                                      CAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGG              >  SRR19973065.995341/1‑150 (MQ=60)
                                                                                                                                      CAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGG              >  SRR19973065.3037466/1‑150 (MQ=60)
                                                                                                                                       AAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGC             >  SRR19973065.2690768/1‑150 (MQ=60)
                                                                                                                                       AAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGAGACCGCCAAGCAGGCC             >  SRR19973065.2694221/1‑150 (MQ=60)
                                                                                                                                          CGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAG          <  SRR19973065.297042/150‑1 (MQ=60)
                                                                                                                                            CCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGA        >  SRR19973065.2954788/1‑150 (MQ=60)
                                                                                                                                            CCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGA        >  SRR19973065.3033224/1‑150 (MQ=60)
                                                                                                                                              TGGCTGGTGTCATCGCGCTCTTCGGTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCAGGGTGCCCCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATC      <  SRR19973065.3440867/150‑1 (MQ=60)
                                                                                                                                              TGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGCGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATC      >  SRR19973065.694878/1‑150 (MQ=60)
                                                                                                                                                  GGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCG  <  SRR19973065.1133644/150‑1 (MQ=60)
                                                                                                                                                  GGGTGTCATCGCGCTCTGCGGGGTTTAGTTTTTTTGGCACACGGTCACTTGAACGGGAGCTTCCCGCCAGTTGCAGCAGAGAATCAAACAACGCGTACCAGGGTGCCGCGGTTTGTTTTACGCGACCGCCAAGCAGGGCGAGGATCGGCG  <  SRR19973065.3250884/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
TCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCG  >  CP009273/1530883‑1531178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑