Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,424,363 T→A 100% T138S (ACC→TCC)  tsaC ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,424,3630TA97.9% 624.8 / 2.2 192T138S (ACC→TCC) tsaCtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (103/85);  total (106/86)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 194 total aligned reads displayed.

AGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGG  >  CP009273/3424218‑3424507
                                                                                                                                                 |                                                                                                                                                
  ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                              >  1:343620/1‑148 (MQ=255)
  ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCAACTCAAGTTGGCACTGgaagaa                                                                                                                                              >  1:344788/1‑148 (MQ=255)
   tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGaaa                                                                                                                                             >  2:190337/1‑148 (MQ=255)
   tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                             <  2:1287088/148‑1 (MQ=255)
   tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                             >  1:3130730/1‑148 (MQ=255)
   tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                             <  2:3498523/148‑1 (MQ=255)
   tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                             >  1:1743385/1‑148 (MQ=255)
    cACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAAc                                                                                                                                            >  1:352589/1‑148 (MQ=255)
     aCCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAAcc                                                                                                                                           >  1:1345075/1‑148 (MQ=255)
           cAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAg                                                                                                                                         <  1:820517/144‑1 (MQ=255)
           cAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAg                                                                                                                                         >  2:820259/1‑144 (MQ=255)
            aGGGCATCGCGGATTTCTGAAGTATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGtt                                                                                                                                    <  1:2486386/148‑1 (MQ=255)
              ggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTa                                                                                                                                  >  1:671028/1‑148 (MQ=255)
              ggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTa                                                                                                                                  >  1:2229967/1‑148 (MQ=255)
                cATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTAcc                                                                                                                                <  1:1502633/148‑1 (MQ=255)
                  tCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAt                                                                                                                              >  2:191555/1‑148 (MQ=255)
                  tCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAt                                                                                                                              >  2:191697/1‑148 (MQ=255)
                  tCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAt                                                                                                                              >  2:1991528/1‑148 (MQ=255)
                    gcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATaa                                                                                                                            <  1:1490026/148‑1 (MQ=255)
                       gATTTCTTATGGATTTAAACTCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGcc                                                                                                                         <  2:2993371/148‑1 (MQ=255)
                       gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGcc                                                                                                                         <  2:1966983/148‑1 (MQ=255)
                        aTTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                        <  1:462146/148‑1 (MQ=255)
                        aTTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                        >  2:1187750/1‑148 (MQ=255)
                               aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                 <  1:219887/148‑1 (MQ=255)
                                 ggATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAg                                                                                                               <  2:155403/148‑1 (MQ=255)
                                 ggATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAg                                                                                                               >  2:140307/1‑148 (MQ=255)
                                   aTTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCa                                                                                                             >  2:2398068/1‑148 (MQ=255)
                                        aaCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccac                                                                                                        <  1:3339588/148‑1 (MQ=255)
                                        aaCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccac                                                                                                        <  2:2471681/148‑1 (MQ=255)
                                         aCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccacc                                                                                                       >  2:772719/1‑148 (MQ=255)
                                         aCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccacc                                                                                                       >  1:1636382/1‑148 (MQ=255)
                                          cGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAccacca                                                                                                      >  1:2422973/1‑148 (MQ=255)
                                          cGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAccacca                                                                                                      >  1:832400/1‑148 (MQ=255)
                                          cGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAccacca                                                                                                      >  2:640387/1‑148 (MQ=255)
                                          cGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAccacca                                                                                                      >  1:3328884/1‑148 (MQ=255)
                                               ccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGa                                                                                                 <  1:2460095/148‑1 (MQ=255)
                                               ccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGa                                                                                                 <  1:2742529/148‑1 (MQ=255)
                                                cccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGAt                                                                                                >  2:373257/1‑148 (MQ=255)
                                                cccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGAt                                                                                                >  2:3278605/1‑148 (MQ=255)
                                                cccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGAt                                                                                                <  1:2856652/148‑1 (MQ=255)
                                                 ccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATg                                                                                               >  2:345826/1‑148 (MQ=255)
                                                  cGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATgg                                                                                              >  2:661483/1‑148 (MQ=255)
                                                     ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCg                                                                                           >  1:1303864/1‑148 (MQ=255)
                                                        aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTg                                                                                        >  2:3041866/1‑148 (MQ=255)
                                                        aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTg                                                                                        >  1:760829/1‑148 (MQ=255)
                                                             gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                   >  2:893/1‑148 (MQ=255)
                                                             gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                   >  2:1873542/1‑148 (MQ=255)
                                                             gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                   >  1:3446589/1‑148 (MQ=255)
                                                             gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                   >  2:2606071/1‑148 (MQ=255)
                                                                caACCGGGAACGCCGCGCCAACTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTAc                                                                                <  2:106433/148‑1 (MQ=255)
                                                                caACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTAc                                                                                >  2:1715682/1‑148 (MQ=255)
                                                                caACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTAc                                                                                <  2:106411/148‑1 (MQ=255)
                                                                caACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTAc                                                                                <  2:107006/148‑1 (MQ=255)
                                                                   ccGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGc                                                                             >  2:1993533/1‑148 (MQ=255)
                                                                    cGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCa                                                                            >  2:2529199/1‑148 (MQ=255)
                                                                               cgcCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCaaa                                                                 <  2:1743900/148‑1 (MQ=255)
                                                                                   aaaTTGTGCGCGATTTTCGTCTACTTTTCTAAATGTTGTAAATCAACTAAAGTTGTCACTGGAATAAACCAGCGGTTTACCATAAGCCGGGCACAATGCAACAACCAACGGTTGTTCGGTGACTCGTACAGCAAGCTAATCAAAGCgg                                                             <  2:3426508/148‑1 (MQ=255)
                                                                                     aTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGcc                                                           >  2:287870/1‑148 (MQ=255)
                                                                                     aTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGcc                                                           >  1:2504597/1‑148 (MQ=255)
                                                                                       tgtgCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCg                                                         >  2:1301594/1‑148 (MQ=255)
                                                                                       tgtgCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCg                                                         >  2:1675598/1‑148 (MQ=255)
                                                                                        gtgCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGt                                                        <  2:1129080/148‑1 (MQ=255)
                                                                                          gcgcGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCa                                                      >  1:1748739/1‑148 (MQ=255)
                                                                                               aaCTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAg                                                 >  1:590107/1‑148 (MQ=255)
                                                                                               aaCTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAg                                                 >  1:3221416/1‑148 (MQ=255)
                                                                                                 attCGTCTACTGTTCTACATGGTGTCAATCCACTCAAGTTGGCACTGTAATAAACCAGGGTTTTACCATAAGCCTGGCACAACGCAACCACCAACGTTTGGTCGTTGACTCGTACCGCAAGCTAATCAAAGCGGCCCGTCAACCAgcg                                               <  1:2728999/147‑1 (MQ=255)
                                                                                                    cGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCgg                                            <  2:1345075/148‑1 (MQ=255)
                                                                                                     gTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCACGCGAATCAAAGCGGCCCGTCAACCAGCGCGgt                                           <  2:2718808/148‑1 (MQ=255)
                                                                                                       cTACTTTTCGACATGGTGGCAATCCACTCAAGTTGGCACTGGAACAAACCAGCGTTTTACAATAAGCCTGCCACAAACCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtgt                                         <  1:1851888/148‑1 (MQ=255)
                                                                                                       cTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtgt                                         >  1:2497277/1‑148 (MQ=255)
                                                                                                         aCTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGtgttg                                       <  1:2007486/148‑1 (MQ=255)
                                                                                                         aCTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttg                                       <  2:3156658/148‑1 (MQ=255)
                                                                                                          cTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttgt                                      >  2:1005272/1‑148 (MQ=255)
                                                                                                          cTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttgt                                      <  2:787432/148‑1 (MQ=255)
                                                                                                           tGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTc                                     >  2:2335748/1‑148 (MQ=255)
                                                                                                              tCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCa                                  >  1:2421654/1‑148 (MQ=255)
                                                                                                                 aCAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGgc                               >  2:2495234/1‑148 (MQ=255)
                                                                                                                  cAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGgcg                              >  2:1437437/1‑148 (MQ=255)
                                                                                                                   atggTGGCAATCCACTCAATTTGGCACTGGAAGAAACCAGGGGTTTACCATACGCCGGGCACAAAGCAACCACCACCGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGgcgc                             <  1:1002281/146‑1 (MQ=255)
                                                                                                                    aGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGcgcg                            >  1:1047134/1‑148 (MQ=255)
                                                                                                                     ggTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCgg                           >  1:1136980/1‑148 (MQ=255)
                                                                                                                      gTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCggg                          >  1:3249075/1‑148 (MQ=255)
                                                                                                                         gCAATCAACTCAAGTGGGCACTGTAATAAACCAGCGTTTTACCATACGCCGGGCACAAACCAACCCCCACCGGTGGGTGGGTGACTCGTACAGCACGCTAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaaa                       <  1:1206607/148‑1 (MQ=255)
                                                                                                                          cAATCCACTCCAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGTTGGTCGGTGACTCGTACCGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAg                      <  1:1767439/148‑1 (MQ=255)
                                                                                                                          cAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAg                      >  1:1920558/1‑148 (MQ=255)
                                                                                                                                 cTCAAGTTGGCAGTGGAAGAAACCAGCGTTTTACCATACGCCTGGCACAACGCAACCACCAACGGTTGGGCGGTGACTCTTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAgg               <  1:2837830/148‑1 (MQ=255)
                                                                                                                                 cTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAgg               <  2:2647029/148‑1 (MQ=255)
                                                                                                                                 cTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAgg               >  1:3369038/1‑148 (MQ=255)
                                                                                                                                  tCAAGTTGCCAGTGTAAGAAACCAGGGGTTTACAATACGCCTGGCACAAACCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAAAAAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGt              <  1:84230/148‑1 (MQ=255)
                                                                                                                                   cAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTg             <  2:3393693/148‑1 (MQ=255)
                                                                                                                                     tgtgggCACTGGAAGAAACCAGCGGTTTACCATACGCCTGGCACAAAGCAACCACCACCGGTTGGTCGGTGACTCGTACCGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGAc           <  1:716109/144‑1 (MQ=255)
                                                                                                                                     aGTTGGCACTGGAAGAAACCTGCGTTTTACCATACGCCTGGCACAAAGCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGAc           <  1:2137155/148‑1 (MQ=255)
                                                                                                                                      gTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAACGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACa          <  2:2101467/148‑1 (MQ=255)
                                                                                                                                      gTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACa          <  2:2103565/148‑1 (MQ=255)
                                                                                                                                       ttGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAg         >  2:525452/1‑148 (MQ=255)
                                                                                                                                           cACTGTAAGAAACCAGCGTTTTACCATAAGCCTGGCACAAAGCAACCACAAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAcc     <  2:1262553/148‑1 (MQ=255)
                                                                                                                                           cACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAcc     <  1:2939985/148‑1 (MQ=255)
                                                                                                                                             cTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTg   <  2:394501/148‑1 (MQ=255)
                                                                                                                                             cTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTg   >  1:799717/1‑148 (MQ=255)
                                                                                                                                              tGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTgg  <  1:140307/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGG  >  CP009273/3424218‑3424507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC  >  CP009273/3424215‑3424509
                                                                                                                                                    |                                                                                                                                                  
AACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAA                                                                                                                                                   >  SRR19973066.1937082/1‑150 (MQ=60)
 ACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAG                                                                                                                                                  >  SRR19973066.1924333/1‑150 (MQ=60)
   AGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAA                                                                                                                                                >  SRR19973066.346281/1‑150 (MQ=60)
   AGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCAACTCAAGTTGGCACTGGAAGAA                                                                                                                                                >  SRR19973066.347462/1‑150 (MQ=60)
    GTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAA                                                                                                                                               >  SRR19973066.3151628/1‑150 (MQ=60)
    GTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAA                                                                                                                                               >  SRR19973066.1756461/1‑150 (MQ=60)
    GTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAA                                                                                                                                               >  SRR19973066.1755945/1‑150 (MQ=60)
     TTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAAC                                                                                                                                              >  SRR19973066.355338/1‑150 (MQ=60)
     TTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAAC                                                                                                                                              >  SRR19973066.3473994/1‑150 (MQ=60)
      TCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACC                                                                                                                                             >  SRR19973066.1354828/1‑150 (MQ=60)
      TCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACC                                                                                                                                             >  SRR19973066.973106/1‑150 (MQ=60)
          gtGTCAGGGCATCGGGTATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCG                                                                                                                                         <  SRR19973066.826256/148‑1 (MQ=60)
          ctttccGGGCATCGGGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCG                                                                                                                                         <  SRR19973066.826516/144‑1 (MQ=60)
           CGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGG                                                                                                                                        >  SRR19973066.6761/1‑150 (MQ=60)
               AGGGCATCGCGGATTTCTGAAGTATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                    <  SRR19973066.2503349/150‑1 (MQ=60)
               AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                    >  SRR19973066.675934/1‑150 (MQ=60)
               AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                    >  SRR19973066.2245366/1‑150 (MQ=60)
               AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                    >  SRR19973066.328095/1‑150 (MQ=60)
               AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                    >  SRR19973066.676105/1‑150 (MQ=60)
               AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                    >  SRR19973066.737180/1‑150 (MQ=60)
                   CATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAT                                                                                                                                <  SRR19973066.1513100/150‑1 (MQ=60)
                    ATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATA                                                                                                                               >  SRR19973066.3465999/1‑150 (MQ=60)
                      CGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAG                                                                                                                             <  SRR19973066.783019/150‑1 (MQ=60)
                       GCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGC                                                                                                                            <  SRR19973066.1500457/150‑1 (MQ=60)
                       GCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGC                                                                                                                            <  SRR19973066.1500470/150‑1 (MQ=60)
                       GCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGC                                                                                                                            <  SRR19973066.1497231/150‑1 (MQ=60)
                          GATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGGGCAACCGGGGACGCCGCGCCCACTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTG                                                                                                                         >  SRR19973066.3421429/1‑150 (MQ=60)
                           ATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGG                                                                                                                        <  SRR19973066.465680/150‑1 (MQ=60)
                               CTGATGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACA                                                                                                                    <  SRR19973066.1739620/150‑1 (MQ=60)
                               CTGAAGTATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACA                                                                                                                    <  SRR19973066.2768961/150‑1 (MQ=60)
                                  AAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAG                                                                                                                 <  SRR19973066.2928978/150‑1 (MQ=60)
                                  AAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAG                                                                                                                 <  SRR19973066.221680/150‑1 (MQ=60)
                                          AAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACC                                                                                                         >  SRR19973066.1648277/1‑150 (MQ=60)
                                           AACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCA                                                                                                        >  SRR19973066.837955/1‑150 (MQ=60)
                                           AACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCA                                                                                                        >  SRR19973066.838469/1‑150 (MQ=60)
                                           AACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCA                                                                                                        >  SRR19973066.3351002/1‑150 (MQ=60)
                                           AACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCA                                                                                                        >  SRR19973066.2439423/1‑150 (MQ=60)
                                           AACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCA                                                                                                        <  SRR19973066.3361820/150‑1 (MQ=60)
                                                  CCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATG                                                                                                 <  SRR19973066.2476858/150‑1 (MQ=60)
                                                  CCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATG                                                                                                 <  SRR19973066.2760936/150‑1 (MQ=60)
                                                  CCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATG                                                                                                 <  SRR19973066.2760982/150‑1 (MQ=60)
                                                  CCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATG                                                                                                 <  SRR19973066.2476862/150‑1 (MQ=60)
                                                  CCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATG                                                                                                 <  SRR19973066.2089044/150‑1 (MQ=60)
                                                   CCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGG                                                                                                <  SRR19973066.2875840/150‑1 (MQ=60)
                                                     CGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTC                                                                                              <  SRR19973066.420832/150‑1 (MQ=60)
                                                     CGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTC                                                                                              <  SRR19973066.2227512/150‑1 (MQ=60)
                                                      GNTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCG                                                                                             >  SRR19973066.1313316/1‑150 (MQ=60)
                                                         TCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTG                                                                                          >  SRR19973066.766470/1‑150 (MQ=60)
                                                              AGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCG                                                                                     >  SRR19973066.3469639/1‑150 (MQ=60)
                                                              AGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCG                                                                                     >  SRR19973066.3469658/1‑150 (MQ=60)
                                                                    AACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGC                                                                               >  SRR19973066.3383320/1‑150 (MQ=60)
                                                                    AACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGC                                                                               >  SRR19973066.1603973/1‑150 (MQ=60)
                                                                             CGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATC                                                                      <  SRR19973066.472157/150‑1 (MQ=60)
                                                                                   GCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCG                                                                <  SRR19973066.1859852/150‑1 (MQ=60)
                                                                                     CAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGC                                                              >  SRR19973066.2326876/1‑150 (MQ=60)
                                                                                      AAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCC                                                             >  SRR19973066.2521654/1‑150 (MQ=60)
                                                                                      AAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCC                                                             >  SRR19973066.2521880/1‑150 (MQ=60)
                                                                                           GTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCA                                                        >  SRR19973066.1761321/1‑150 (MQ=60)
                                                                                                CGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAG                                                   >  SRR19973066.594213/1‑150 (MQ=60)
                                                                                                CGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAG                                                   >  SRR19973066.3242727/1‑150 (MQ=60)
                                                                                                CGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAG                                                   >  SRR19973066.3242563/1‑150 (MQ=60)
                                                                                                    ATTCGTCTACTGTTCTACATGGTGTCAATCCACTCAAGTTGGCACTGTAATAAACCAGGGTTTTACCATAAGCCTGGCACAACGCAACCACCAACGTTTGGTCGTTGACTCGTACCGCAAGCTAATCAAAGCGGCCCGTCAACCAGCGCG                                               <  SRR19973066.2747353/150‑1 (MQ=60)
                                                                                                      GCGTCTACTGTTCGACATGGTGCAAAGCCACACAAGTTGGCACTGCAACAAACCAGCGTTTTACCATACGCCTGCCACAACGCAACCACCACCGGTTGGTCGGTGACTCGTACAGAAAGCGAATAAAAGCGGCCCGTCAACCAGCGCGGT                                             <  SRR19973066.2445037/150‑1 (MQ=60)
                                                                                                        GTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGT                                           >  SRR19973066.2514297/1‑150 (MQ=60)
                                                                                                        GTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGT                                           >  SRR19973066.2514468/1‑150 (MQ=60)
                                                                                                          CTACTTTTCGACATGGTGGCAATCCACTCAAGTTGGCACTGGAACAAACCAGCGTTTTACAATAAGCCTGCCACAAACCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTG                                         <  SRR19973066.1865141/150‑1 (MQ=60)
                                                                                                            ACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTC                                       <  SRR19973066.2021570/150‑1 (MQ=60)
                                                                                                             CTTTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGTAAGAAACCAGCGGTTTACCTTAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCTAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCG                                      <  SRR19973066.1325059/150‑1 (MQ=60)
                                                                                                               GTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCA                                    >  SRR19973066.2438117/1‑150 (MQ=60)
                                                                                                               GTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCA                                    >  SRR19973066.2438091/1‑150 (MQ=60)
                                                                                                                    ACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGC                               >  SRR19973066.3137860/1‑150 (MQ=60)
                                                                                                                     CAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCG                              >  SRR19973066.1054830/1‑150 (MQ=60)
                                                                                                                      ATGGTGGCAATCCACTCAATTTGGCACTGGAAGAAACCAGGGGTTTACCATACGCCGGGCACAAAGCAACCACCACCGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGG                             <  SRR19973066.1009670/150‑1 (MQ=60)
                                                                                                                      AAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGG                             >  SRR19973066.1145123/1‑150 (MQ=60)
                                                                                                                      AAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGG                             >  SRR19973066.1145374/1‑150 (MQ=60)
                                                                                                                       AGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGG                            >  SRR19973066.3270397/1‑150 (MQ=60)
                                                                                                                       AGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGG                            >  SRR19973066.3270485/1‑150 (MQ=60)
                                                                                                                           GGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAG                        >  SRR19973066.1934218/1‑150 (MQ=60)
                                                                                                                            GCAATCAACTCAAGTGGGCACTGTAATAAACCAGCGTTTTACCATACGCCGGGCACAAACCAACCCCCACCGGTGGGTGGGTGACTCGTACAGCACGCTAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGA                       <  SRR19973066.1215024/150‑1 (MQ=60)
                                                                                                                             CAATCCACTCCAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGTTGGTCGGTGACTCGTACCGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGAC                      <  SRR19973066.1780110/150‑1 (MQ=60)
                                                                                                                             CAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGAC                      <  SRR19973066.1779752/150‑1 (MQ=60)
                                                                                                                                 CCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAG                  <  SRR19973066.1385548/150‑1 (MQ=60)
                                                                                                                                  CACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGG                 <  SRR19973066.1988026/150‑1 (MQ=60)
                                                                                                                                  CACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGG                 >  SRR19973066.3391511/1‑150 (MQ=60)
                                                                                                                                    CTCAAGTTGGCAGTGGAAGAAACCAGCGTTTTACCATACGCCTGGCACAACGCAACCACCAACGGTTGGGCGGTGACTCTTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTG               <  SRR19973066.2856906/150‑1 (MQ=60)
                                                                                                                                    CTCAAGTTGGAATTGTAAGAAACCAGGGTTTTACAATAAGCCTGGAAAAAAGCAACCACCAAGGGTTGGTGGGTTACTCTTACAGAAAGCTAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTG               <  SRR19973066.1265274/150‑1 (MQ=60)
                                                                                                                                     TCAAGTTGCCAGTGTAAGAAACCAGGGGTTTACAATACGCCTGGCACAAACCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAAAAAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGA              <  SRR19973066.85170/150‑1 (MQ=60)
                                                                                                                                        tgtgGGCACTGGAAGAAACCAGCGGTTTACCATACGCCTGGCACAAAGCAACCACCACCGGTTGGTCGGTGACTCGTACCGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAG           <  SRR19973066.721410/146‑1 (MQ=60)
                                                                                                                                        AGTTGGCACTGGAAGAAACCTGCGTTTTACCATACGCCTGGCACAAAGCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAG           <  SRR19973066.2151981/150‑1 (MQ=60)
                                                                                                                                        AGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGACAGACAAAGGTGACAG           >  SRR19973066.2867757/1‑150 (MQ=60)
                                                                                                                                          TTGGAACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTTACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGA         <  SRR19973066.1084497/150‑1 (MQ=60)
                                                                                                                                           TGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAC        >  SRR19973066.249208/1‑150 (MQ=60)
                                                                                                                                           TGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAC        >  SRR19973066.250997/1‑150 (MQ=60)
                                                                                                                                              CACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG     <  SRR19973066.2959529/150‑1 (MQ=60)
                                                                                                                                              CACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG     >  SRR19973066.805589/1‑150 (MQ=60)
                                                                                                                                               ACTGGAAGAAACCAGCGTTTTACCATAAGCCTGGCACAAAGCACCCCCCAACGGATGGTCGGTTACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGG    <  SRR19973066.2268326/150‑1 (MQ=60)
                                                                                                                                                 TGGAAGAAACCAGCGTTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC  <  SRR19973066.141662/150‑1 (MQ=60)
                                                                                                                                                 TGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC  >  SRR19973066.1316149/1‑150 (MQ=60)
                                                                                                                                                 TGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC  >  SRR19973066.1319258/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
AACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC  >  CP009273/3424215‑3424509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑