Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,424,363 T→A 100% T138S (ACC→TCC)  tsaC ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,424,3630TA99.1% 721.2 / ‑2.5 217T138S (ACC→TCC) tsaCtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (108/107);  total (109/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

TTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG  >  CP009273/3424220‑3424506
                                                                                                                                               |                                                                                                                                               
ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                             <  1:1062328/148‑1 (MQ=255)
ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                             >  2:3713458/1‑148 (MQ=255)
ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                             >  2:1220887/1‑148 (MQ=255)
ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                             >  2:650466/1‑148 (MQ=255)
 tcccccGTCCGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACACCCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                            <  1:1369450/145‑1 (MQ=255)
 tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                            <  1:1311296/148‑1 (MQ=255)
  cACCCGTCAGGGCATCGCGTATTTCTTAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAAc                                                                                                                                           <  1:1471716/148‑1 (MQ=255)
    cccGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGTACTGGAAGAAACCa                                                                                                                                         >  2:3576670/1‑148 (MQ=255)
     ccGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAg                                                                                                                                        <  1:3563235/148‑1 (MQ=255)
        tCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCgg                                                                                                                                     <  1:2415815/148‑1 (MQ=255)
         cAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCACTCCACTCAAGTTGGCACTGGAAGAAACCAGCGGt                                                                                                                                    >  2:3696484/1‑148 (MQ=255)
         cAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGt                                                                                                                                    <  2:3509650/148‑1 (MQ=255)
            ggCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTa                                                                                                                                 >  1:559385/1‑148 (MQ=255)
              cATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTAcc                                                                                                                               <  2:1036444/148‑1 (MQ=255)
               aTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCa                                                                                                                              <  2:1367205/148‑1 (MQ=255)
                tCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAt                                                                                                                             >  1:1927317/1‑148 (MQ=255)
                tCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAt                                                                                                                             >  1:676955/1‑148 (MQ=255)
                 cgcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATa                                                                                                                            <  2:1047724/148‑1 (MQ=255)
                   cgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAg                                                                                                                          >  1:421729/1‑148 (MQ=255)
                    tgATTTCTGATGGATTTAAACGCCGCGCCGTTTCACCATGCACATCGGGGAACGCCGCGCCTATGTGTGCGCGAACTTCGTCTACTGTTCGACAAGTTGGCTATCCTCTCAAGTTGGCACTGTAAGATACGAGGGGTTTACCATaatc                                                                                                                         <  2:3402829/147‑3 (MQ=255)
                     gATTTCTGAAGGATTTAAACG‑CCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCCGCGGTTTACCATAAGCCt                                                                                                                       >  2:1638840/1‑148 (MQ=255)
                      aTTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                       >  2:3770166/1‑148 (MQ=255)
                          cTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGca                                                                                                                   <  1:848122/148‑1 (MQ=255)
                            gAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGcaca                                                                                                                 >  2:2008921/1‑148 (MQ=255)
                             aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                >  1:2053949/1‑148 (MQ=255)
                              aGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaaa                                                                                                               >  1:2254514/1‑148 (MQ=255)
                              aGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaaa                                                                                                               >  1:2145445/1‑148 (MQ=255)
                              aGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaaa                                                                                                               <  1:2765388/148‑1 (MQ=255)
                              aGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaaa                                                                                                               <  1:847691/148‑1 (MQ=255)
                                 aTTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCa                                                                                                            <  1:2213577/148‑1 (MQ=255)
                                  tttAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCaa                                                                                                           >  1:2351068/1‑148 (MQ=255)
                                   ttAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAac                                                                                                          >  1:97386/1‑148 (MQ=255)
                                    tAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGGGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCATGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAacc                                                                                                         <  1:3337543/148‑1 (MQ=255)
                                     aaaCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAacca                                                                                                        >  2:2926738/1‑148 (MQ=255)
                                       aCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccacc                                                                                                      >  1:1908111/1‑148 (MQ=255)
                                       aCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccacc                                                                                                      >  2:1341889/1‑148 (MQ=255)
                                            cccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACgg                                                                                                 <  2:1321682/148‑1 (MQ=255)
                                            cccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACgg                                                                                                 >  1:1768053/1‑148 (MQ=255)
                                                cGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATgg                                                                                             >  1:2631812/1‑148 (MQ=255)
                                                cGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATgg                                                                                             <  2:1201374/148‑1 (MQ=255)
                                                  tttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTc                                                                                           >  2:3010511/1‑148 (MQ=255)
                                                   ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCg                                                                                          >  2:494015/1‑148 (MQ=255)
                                                   ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCg                                                                                          >  1:2139734/1‑148 (MQ=255)
                                                    tCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCgg                                                                                         <  2:1124698/148‑1 (MQ=255)
                                                      aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTg                                                                                       >  1:3680234/1‑148 (MQ=255)
                                                      aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTg                                                                                       <  1:3690162/148‑1 (MQ=255)
                                                        cAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGAc                                                                                     <  2:780660/148‑1 (MQ=255)
                                                        cAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGAc                                                                                     <  1:2361225/148‑1 (MQ=255)
                                                         aGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACt                                                                                    >  2:2145181/1‑148 (MQ=255)
                                                           gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACCAGGTGGCAATCCACTAAAGTTGGCAATGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                  >  2:243153/1‑148 (MQ=255)
                                                           gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAANTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                  >  1:1938929/1‑148 (MQ=255)
                                                           gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                  >  2:3120202/1‑148 (MQ=255)
                                                            cacaACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGt                                                                                 <  2:2005468/148‑1 (MQ=255)
                                                                aCCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAg                                                                             >  1:3401077/1‑148 (MQ=255)
                                                                    ggAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAg                                                                         <  1:3814933/148‑1 (MQ=255)
                                                                    acgaCGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAg                                                                         >  2:778114/4‑148 (MQ=255)
                                                                     gAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACAAACGGATGGTCGGTGACTCGTACAGCAAGc                                                                        >  1:2677035/1‑148 (MQ=255)
                                                                      aaCGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCg                                                                       <  2:1540207/148‑1 (MQ=255)
                                                                      aaCGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCg                                                                       >  1:3728852/1‑148 (MQ=255)
                                                                        cgccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGaa                                                                     <  2:2230840/148‑1 (MQ=255)
                                                                        cgccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGaa                                                                     <  1:3296719/148‑1 (MQ=255)
                                                                              gcCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAg                                                               <  1:314459/148‑1 (MQ=255)
                                                                                cAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCg                                                             >  2:633073/1‑148 (MQ=255)
                                                                                  aaTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGc                                                           <  2:2515877/148‑1 (MQ=255)
                                                                                           cgAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAAcc                                                  >  1:2372026/1‑148 (MQ=255)
                                                                                               cTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAgcg                                              >  1:165909/1‑148 (MQ=255)
                                                                                                 tCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGcgcg                                            >  1:888523/1‑148 (MQ=255)
                                                                                                 tCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGcgcg                                            <  2:1977561/148‑1 (MQ=255)
                                                                                                 tCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGcgcg                                            >  2:3689881/1‑148 (MQ=255)
                                                                                                 tCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGcgcg                                            <  2:45970/148‑1 (MQ=255)
                                                                                                    tCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtg                                         <  2:1839829/148‑1 (MQ=255)
                                                                                                    tCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtg                                         <  2:421729/148‑1 (MQ=255)
                                                                                                       aCTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttg                                      >  2:2731683/1‑148 (MQ=255)
                                                                                                       aCTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttg                                      >  2:3103505/1‑148 (MQ=255)
                                                                                                       aCTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttg                                      <  1:269669/148‑1 (MQ=255)
                                                                                                       aCTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttg                                      >  1:3183614/1‑148 (MQ=255)
                                                                                                        cTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttgt                                     >  1:3209563/1‑148 (MQ=255)
                                                                                                          gTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCg                                   >  2:2267723/1‑148 (MQ=255)
                                                                                                           ttCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGc                                  >  2:714110/1‑148 (MQ=255)
                                                                                                            tCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCa                                 <  2:2999045/148‑1 (MQ=255)
                                                                                                            tCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCa                                 >  2:3380944/1‑148 (MQ=255)
                                                                                                            acGACAAGGTGCCAATCCACTCAAGTGCGCACTGGAAGAAACCCGCGGTTTACCATAAGCCTGGCACAAAGCACCCACCAACGGATGGACGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCa                                 <  2:3210836/147‑1 (MQ=255)
                                                                                                                   ggTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCgg                          >  1:3344299/1‑148 (MQ=255)
                                                                                                                   ggTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCgg                          >  2:1719588/1‑148 (MQ=255)
                                                                                                                       gCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaaa                      >  2:1299670/1‑148 (MQ=255)
                                                                                                                        cAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAg                     <  2:3658145/148‑1 (MQ=255)
                                                                                                                         aaTCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGa                    <  1:1220887/148‑1 (MQ=255)
                                                                                                                            ccACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACaa                 <  2:614603/148‑1 (MQ=255)
                                                                                                                               cTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAgg              >  1:3106938/1‑148 (MQ=255)
                                                                                                                                 cAAGTTGGCACTGGAAGAAACCAGCTGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTg            <  2:3311592/148‑1 (MQ=255)
                                                                                                                                 cAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTg            <  2:3680234/148‑1 (MQ=255)
                                                                                                                                  aaGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGa           <  2:1593371/148‑1 (MQ=255)
                                                                                                                                    gTTTGCACTGGAAGAAACCAGCGTTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACa         <  2:2199386/148‑1 (MQ=255)
                                                                                                                                    gTTGGCACTGTAAGAAACCAGGGTTTTACAATACCCCTGGCAAAAAGAAACCACCAAGGGTTGGTCGGTGACTCGTACACAAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACa         <  1:1634990/148‑1 (MQ=255)
                                                                                                                                       ggCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGTATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGa      <  2:794425/148‑1 (MQ=255)
                                                                                                                                        gCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAc     <  2:2585033/148‑1 (MQ=255)
                                                                                                                                         cACTGGAAGAAACCAGCGTTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAcc    <  2:572663/148‑1 (MQ=255)
                                                                                                                                         cACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAcc    <  1:2926745/148‑1 (MQ=255)
                                                                                                                                         cACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAcc    <  2:766101/148‑1 (MQ=255)
                                                                                                                                           cTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTg  >  1:1300057/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG  >  CP009273/3424220‑3424506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCAG  >  CP009273/3424214‑3424511
                                                                                                                                                     |                                                                                                                                                    
AAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGA                                                                                                                                                      >  SRR19973069.3481794/1‑150 (MQ=60)
AAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGA                                                                                                                                                      >  SRR19973069.3630799/1‑150 (MQ=60)
  ACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAG                                                                                                                                                    <  SRR19973069.245284/150‑1 (MQ=60)
  ACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAG                                                                                                                                                    <  SRR19973069.828966/150‑1 (MQ=60)
   CAGTTCCCCCGTCAGGGCATCGCGGATTTCTGACGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGA                                                                                                                                                   <  SRR19973069.1971647/150‑1 (MQ=60)
   CAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGA                                                                                                                                                   <  SRR19973069.1971664/150‑1 (MQ=60)
    AGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAA                                                                                                                                                  >  SRR19973069.3553832/1‑150 (MQ=60)
      TTCCCCCGTCAGGGCATCGCGGATTTGTGAATGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAAC                                                                                                                                                <  SRR19973069.2342732/150‑1 (MQ=60)
      TTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAAC                                                                                                                                                <  SRR19973069.1069728/150‑1 (MQ=60)
       TCCCCCGTCCGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACACCCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACC                                                                                                                                               <  SRR19973069.1378978/150‑1 (MQ=60)
       TCCCCCGTCAGGCCATGGGGGATTTCTGATGTATTTACACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACC                                                                                                                                               <  SRR19973069.1320477/150‑1 (MQ=60)
       TCACCCGTCAGGGCATCGCGGTTTTCTGATGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACC                                                                                                                                               <  SRR19973069.1320266/150‑1 (MQ=60)
        CACCCGTCAGGGCATCGCGTATTTCTTAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCA                                                                                                                                              <  SRR19973069.1481973/150‑1 (MQ=60)
          CCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGC                                                                                                                                            >  SRR19973069.1751879/1‑150 (MQ=60)
           CCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCG                                                                                                                                           <  SRR19973069.3586435/150‑1 (MQ=60)
             GTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGT                                                                                                                                         >  SRR19973069.2602240/1‑150 (MQ=60)
             GTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGT                                                                                                                                         >  SRR19973069.2214320/1‑150 (MQ=60)
             GTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGT                                                                                                                                         >  SRR19973069.2115804/1‑150 (MQ=60)
             GTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGT                                                                                                                                         >  SRR19973069.2115125/1‑150 (MQ=60)
              TCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTT                                                                                                                                        <  SRR19973069.2432138/150‑1 (MQ=60)
                AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                      >  SRR19973069.563280/1‑150 (MQ=60)
                  GGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACC                                                                                                                                    <  SRR19973069.3447003/150‑1 (MQ=60)
                    CATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAT                                                                                                                                  >  SRR19973069.681646/1‑150 (MQ=60)
                    CATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAT                                                                                                                                  >  SRR19973069.1940564/1‑150 (MQ=60)
                    CATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAT                                                                                                                                  >  SRR19973069.1940559/1‑150 (MQ=60)
                    CATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCAT                                                                                                                                  >  SRR19973069.1940485/1‑150 (MQ=60)
                       CGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAG                                                                                                                               >  SRR19973069.424826/1‑150 (MQ=60)
                       CGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAG                                                                                                                               <  SRR19973069.2155275/150‑1 (MQ=60)
                         CGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCC                                                                                                                             >  SRR19973069.3844541/1‑150 (MQ=60)
                          GTATTTCTGAAGTATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCT                                                                                                                            <  SRR19973069.715108/150‑1 (MQ=60)
                            ATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGG                                                                                                                          <  SRR19973069.573871/150‑1 (MQ=60)
                            ATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGG                                                                                                                          >  SRR19973069.504870/1‑150 (MQ=60)
                                CTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACA                                                                                                                      <  SRR19973069.854069/150‑1 (MQ=60)
                                CTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACA                                                                                                                      <  SRR19973069.854095/150‑1 (MQ=60)
                                 TGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAA                                                                                                                     >  SRR19973069.2068201/1‑150 (MQ=60)
                                  GAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAA                                                                                                                    >  SRR19973069.2160164/1‑150 (MQ=60)
                                  GAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAA                                                                                                                    >  SRR19973069.2269742/1‑150 (MQ=60)
                                    AGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGC                                                                                                                  <  SRR19973069.853638/150‑1 (MQ=60)
                                    AGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGC                                                                                                                  <  SRR19973069.2783834/150‑1 (MQ=60)
                                    AGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGC                                                                                                                  <  SRR19973069.853634/150‑1 (MQ=60)
                                      GATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAA                                                                                                                >  SRR19973069.2367057/1‑150 (MQ=60)
                                       ATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAC                                                                                                               >  SRR19973069.98373/1‑150 (MQ=60)
                                       ATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAC                                                                                                               <  SRR19973069.2228573/150‑1 (MQ=60)
                                       ATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAAC                                                                                                               <  SRR19973069.2229546/150‑1 (MQ=60)
                                          TAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGGGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCATGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCAC                                                                                                            <  SRR19973069.3359397/150‑1 (MQ=60)
                                          TAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCAC                                                                                                            <  SRR19973069.3359458/150‑1 (MQ=60)
                                           AAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACC                                                                                                           >  SRR19973069.1921288/1‑150 (MQ=60)
                                              CGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAAC                                                                                                        >  SRR19973069.3333297/1‑150 (MQ=60)
                                              CGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAAC                                                                                                        >  SRR19973069.1270885/1‑150 (MQ=60)
                                                CCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGG                                                                                                      >  SRR19973069.3682097/1‑150 (MQ=60)
                                                CCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGG                                                                                                      >  SRR19973069.2675362/1‑150 (MQ=60)
                                                CCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGG                                                                                                      >  SRR19973069.1780512/1‑150 (MQ=60)
                                                   CCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATG                                                                                                   >  SRR19973069.1311240/1‑150 (MQ=60)
                                                    CCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGG                                                                                                  >  SRR19973069.2649438/1‑150 (MQ=60)
                                                      CGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTC                                                                                                >  SRR19973069.957553/1‑150 (MQ=60)
                                                      CGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTC                                                                                                >  SRR19973069.1218543/1‑150 (MQ=60)
                                                      CGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTC                                                                                                >  SRR19973069.233534/1‑150 (MQ=60)
                                                      CGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTC                                                                                                >  SRR19973069.2882163/1‑150 (MQ=60)
                                                       GTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCG                                                                                               >  SRR19973069.2154427/1‑150 (MQ=60)
                                                          TCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTG                                                                                            >  SRR19973069.3704310/1‑150 (MQ=60)
                                                          TCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTG                                                                                            >  SRR19973069.3704307/1‑150 (MQ=60)
                                                           CACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGA                                                                                           >  SRR19973069.1372568/1‑150 (MQ=60)
                                                            ACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGAC                                                                                          <  SRR19973069.3714295/150‑1 (MQ=60)
                                                              CAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTC                                                                                        <  SRR19973069.2377215/150‑1 (MQ=60)
                                                              CAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTC                                                                                        <  SRR19973069.2377271/150‑1 (MQ=60)
                                                              CAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTC                                                                                        <  SRR19973069.2377684/150‑1 (MQ=60)
                                                               AGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAANTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCG                                                                                       >  SRR19973069.1952274/1‑150 (MQ=60)
                                                               AGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCG                                                                                       >  SRR19973069.1953125/1‑150 (MQ=60)
                                                               AGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCG                                                                                       >  SRR19973069.3406755/1‑150 (MQ=60)
                                                               AGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCG                                                                                       >  SRR19973069.1659228/1‑150 (MQ=60)
                                                                    CAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAG                                                                                  >  SRR19973069.735909/1‑150 (MQ=60)
                                                                    CAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAG                                                                                  >  SRR19973069.3423262/1‑150 (MQ=60)
                                                                         GGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACAAACGGATGGTCGGTGACTCGTACAGCAAGC                                                                             >  SRR19973069.2694983/1‑150 (MQ=60)
                                                                          GGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCG                                                                            >  SRR19973069.3753173/1‑150 (MQ=60)
                                                                          GGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCG                                                                            >  SRR19973069.3754463/1‑150 (MQ=60)
                                                                          GGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCG                                                                            <  SRR19973069.3836022/150‑1 (MQ=60)
                                                                          GGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCG                                                                            <  SRR19973069.3839627/150‑1 (MQ=60)
                                                                              CGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATC                                                                        <  SRR19973069.3318300/150‑1 (MQ=60)
                                                                              CGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATC                                                                        <  SRR19973069.3318303/150‑1 (MQ=60)
                                                                              CGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATC                                                                        >  SRR19973069.1645359/1‑150 (MQ=60)
                                                                                    GCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCG                                                                  <  SRR19973069.316546/150‑1 (MQ=60)
                                                                                    GCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCG                                                                  <  SRR19973069.317329/150‑1 (MQ=60)
                                                                                               CGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACC                                                       >  SRR19973069.2388122/1‑150 (MQ=60)
                                                                                               CGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACC                                                       >  SRR19973069.3252420/1‑150 (MQ=60)
                                                                                                 CGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAG                                                     >  SRR19973069.3129875/1‑150 (MQ=60)
                                                                                                   AACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCG                                                   >  SRR19973069.167338/1‑150 (MQ=60)
                                                                                                     CTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCG                                                 >  SRR19973069.894661/1‑150 (MQ=60)
                                                                                                          TATACTGTTCTACAAGGTGGAAACCCACACAAGTTGGCACTGCAAAAAACCAGGTTTTTACAATAAGCCTGCCACAAACAAACCACAAACGTTTGGTCGGTGACTCGTACAGCAAGCTAATCAACGCGGCCCGTCAACCAGCGCGGTGTT                                            <  SRR19973069.2581726/150‑1 (MQ=42)
                                                                                                           CTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTG                                           >  SRR19973069.1793592/1‑150 (MQ=60)
                                                                                                           CTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTG                                           >  SRR19973069.3204311/1‑150 (MQ=60)
                                                                                                           CTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTG                                           >  SRR19973069.1793580/1‑150 (MQ=60)
                                                                                                           CTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTGG                                           >  SRR19973069.1393766/1‑150 (MQ=60)
                                                                                                            TACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGT                                          >  SRR19973069.2419783/1‑150 (MQ=60)
                                                                                                            TACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGT                                          >  SRR19973069.3230481/1‑150 (MQ=60)
                                                                                                            TACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGGGTTGT                                          >  SRR19973069.3230190/1‑150 (MQ=60)
                                                                                                             ACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGAGGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTC                                         <  SRR19973069.3110528/150‑1 (MQ=60)
                                                                                                             ACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTC                                         <  SRR19973069.271583/150‑1 (MQ=60)
                                                                                                             ACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTC                                         <  SRR19973069.271592/150‑1 (MQ=60)
                                                                                                             ACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACAATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTC                                         <  SRR19973069.271577/150‑1 (MQ=60)
                                                                                                                       AAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGG                               >  SRR19973069.3366191/1‑150 (MQ=60)
                                                                                                                        AGGTGGCAATCCACTCATGTTGGCACTGGAAGAAACCAGCGGTTTACCATACGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGG                              <  SRR19973069.2435249/150‑1 (MQ=60)
                                                                                                                               AATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACA                       <  SRR19973069.1229238/150‑1 (MQ=60)
                                                                                                                                   CACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGG                   >  SRR19973069.3127377/1‑150 (MQ=60)
                                                                                                                                    ACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACAATACGCCTGGCACAAAGCAACCACCAACGGTTGGTGGGTGACTCGTACAGCAAGCTAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGT                  <  SRR19973069.1397517/150‑1 (MQ=60)
                                                                                                                                      TCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGA                <  SRR19973069.3720644/150‑1 (MQ=60)
                                                                                                                                          GTTGGCACTGTAAGAAACCAGGGTTTTACAATACCCCTGGCAAAAAGAAACCACCAAGGGTTGGTCGGTGACTCGTACACAAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGG            <  SRR19973069.1646182/150‑1 (MQ=60)
                                                                                                                                               CACTGGAAGAAACCAGCGTTTTACCATACGCGTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCTAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG       <  SRR19973069.2946137/150‑1 (MQ=60)
                                                                                                                                               CACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG       <  SRR19973069.2946144/150‑1 (MQ=60)
                                                                                                                                               CACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG       >  SRR19973069.1308850/1‑150 (MQ=60)
                                                                                                                                                 CTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGC     >  SRR19973069.1421980/1‑150 (MQ=60)
                                                                                                                                                  TGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC    >  SRR19973069.1050274/1‑150 (MQ=60)
                                                                                                                                                  TGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC    >  SRR19973069.1670340/1‑150 (MQ=60)
                                                                                                                                                   GGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCA   >  SRR19973069.1871803/1‑150 (MQ=60)
                                                                                                                                                   GGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCA   >  SRR19973069.1871477/1‑150 (MQ=60)
                                                                                                                                                    GAAGAACCCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGTCTGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCAG  <  SRR19973069.3684741/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCAG  >  CP009273/3424214‑3424511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑