Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,424,363 T→A 100% T138S (ACC→TCC)  tsaC ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,424,3630TA98.9% 607.6 / ‑2.4 184T138S (ACC→TCC) tsaCtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (100/82);  total (102/82)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

TTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGG  >  CP009273/3424220‑3424507
                                                                                                                                               |                                                                                                                                                
ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                              <  2:669247/148‑1 (MQ=255)
ttCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGgaagaa                                                                                                                                              >  1:3253000/1‑148 (MQ=255)
 tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                             >  2:2201261/1‑148 (MQ=255)
 tCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGaaa                                                                                                                                             >  2:1580533/1‑148 (MQ=255)
    cccGTCAGGTCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCa                                                                                                                                          <  2:1230812/148‑1 (MQ=255)
    cccGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCa                                                                                                                                          >  2:2503930/1‑148 (MQ=255)
    cccGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCa                                                                                                                                          <  2:2577842/148‑1 (MQ=255)
     ccGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAg                                                                                                                                         <  1:1371856/148‑1 (MQ=255)
     ccGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAg                                                                                                                                         <  1:3119467/148‑1 (MQ=255)
      cGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGc                                                                                                                                        >  1:1333097/1‑148 (MQ=255)
        tCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCgg                                                                                                                                      >  2:1138413/1‑148 (MQ=255)
        tCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCgg                                                                                                                                      <  1:204237/148‑1 (MQ=255)
         cAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGt                                                                                                                                     >  1:3215147/1‑148 (MQ=255)
          aGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGtt                                                                                                                                    <  1:3468650/148‑1 (MQ=255)
              cagcgcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTAcc                                                                                                                                <  2:181924/145‑1 (MQ=255)
                 cgcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATa                                                                                                                             <  1:2143250/148‑1 (MQ=255)
                 cgcgGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATa                                                                                                                             >  1:42560/1‑148 (MQ=255)
                    ggATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGc                                                                                                                          <  1:3498938/148‑1 (MQ=255)
                     gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGcc                                                                                                                         <  1:558907/148‑1 (MQ=255)
                     gATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGcc                                                                                                                         >  1:2939128/1‑148 (MQ=255)
                      aTTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                        >  1:1515465/1‑148 (MQ=255)
                      aTTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTAGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCt                                                                                                                        >  1:1515636/1‑148 (MQ=255)
                         tCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGc                                                                                                                     >  2:2493811/1‑148 (MQ=255)
                         tCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGc                                                                                                                     <  2:1485448/148‑1 (MQ=255)
                         tCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGc                                                                                                                     <  1:1166354/148‑1 (MQ=255)
                            gAATGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAATTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGcaca                                                                                                                  <  1:3389424/148‑1 (MQ=255)
                            gAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGcaca                                                                                                                  <  1:2460227/148‑1 (MQ=255)
                             acggATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                 <  1:642073/146‑1 (MQ=255)
                             aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTTTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                 >  2:2826326/1‑148 (MQ=255)
                             aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                 <  2:2726321/148‑1 (MQ=255)
                             aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                 >  2:667912/1‑148 (MQ=255)
                             aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                 <  2:499994/148‑1 (MQ=255)
                             aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACaa                                                                                                                 <  1:2265159/148‑1 (MQ=255)
                             aaGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGATGGCACTGGAAGAAACCAGCGGTTTACCATAAGACTGGCACaa                                                                                                                 >  2:1075139/1‑148 (MQ=255)
                                 aTTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCa                                                                                                             >  1:3005703/1‑148 (MQ=255)
                                  tttAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCaa                                                                                                            <  1:2334956/148‑1 (MQ=255)
                                     aaaCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAacca                                                                                                         >  1:2936265/1‑148 (MQ=255)
                                       aCGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAaccacc                                                                                                       >  1:1209314/1‑148 (MQ=255)
                                          cccccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAAc                                                                                                    >  2:185197/1‑148 (MQ=255)
                                           ccccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACg                                                                                                   >  2:3395703/1‑148 (MQ=255)
                                            cccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCgg                                                                                                  >  2:447826/1‑148 (MQ=255)
                                            cccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACgg                                                                                                  >  2:3097690/1‑148 (MQ=255)
                                            cccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACgg                                                                                                  <  2:2331725/148‑1 (MQ=255)
                                             ccccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGa                                                                                                 <  2:2989574/148‑1 (MQ=255)
                                             ccacGTTTCACCAGGCAAAACCGGGAACGCCGCTCCAAATTGTGCGCGAACTTCGTCTAATGTTCGAAAAGGTGGCAATCCACTCAAGTTTGCACTGGAAGAAACCAGCGGTTTACCATAAGCCAGTCACAAAGAAACCACCAACGGa                                                                                                 >  2:124122/4‑148 (MQ=255)
                                               ccGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATg                                                                                               <  2:943227/148‑1 (MQ=255)
                                                cGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATgg                                                                                              <  2:2222869/148‑1 (MQ=255)
                                                 gTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGt                                                                                             >  1:313474/1‑148 (MQ=255)
                                                   ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCg                                                                                           >  1:1177537/1‑148 (MQ=255)
                                                   ttCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCg                                                                                           >  1:3136211/1‑148 (MQ=255)
                                                     cACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGt                                                                                         >  1:591450/1‑148 (MQ=255)
                                                      aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTg                                                                                        >  2:2678985/1‑148 (MQ=255)
                                                      aCCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTg                                                                                        >  2:1398808/1‑148 (MQ=255)
                                                       ccAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGa                                                                                       >  2:862856/1‑148 (MQ=255)
                                                       ccAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGAGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGa                                                                                       >  1:286202/1‑148 (MQ=255)
                                                           gCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCg                                                                                   >  2:1634025/1‑148 (MQ=255)
                                                                 ccGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGc                                                                             >  2:681375/1‑148 (MQ=255)
                                                                   gggAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCaa                                                                           >  1:1249419/1‑148 (MQ=255)
                                                                     gAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGc                                                                         >  1:402487/1‑148 (MQ=255)
                                                                        cgccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGaa                                                                      <  2:3041273/148‑1 (MQ=255)
                                                                        cgccgcGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGaa                                                                      <  2:1008350/148‑1 (MQ=255)
                                                                           cgcgCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCa                                                                   >  2:1454216/1‑148 (MQ=255)
                                                                             cgcCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCaaa                                                                 >  1:833769/1‑148 (MQ=255)
                                                                              gcCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAg                                                                <  1:300484/148‑1 (MQ=255)
                                                                               ccAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGc                                                               <  2:1406637/148‑1 (MQ=255)
                                                                               ccAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGc                                                               >  2:3231683/1‑148 (MQ=255)
                                                                                   aTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGcc                                                           >  1:1685906/1‑148 (MQ=255)
                                                                                         cgcgAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCaa                                                     >  2:2313059/1‑148 (MQ=255)
                                                                                           cgAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAAcc                                                   >  1:2335927/1‑148 (MQ=255)
                                                                                              aCTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAgc                                                >  2:2905578/1‑148 (MQ=255)
                                                                                               cTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAgcg                                               >  1:3428985/1‑148 (MQ=255)
                                                                                                    tCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtg                                          >  1:143122/1‑148 (MQ=255)
                                                                                                    tCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtg                                          >  2:1305480/1‑148 (MQ=255)
                                                                                                     cTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtgt                                         >  2:625286/1‑148 (MQ=255)
                                                                                                     cTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGgtgt                                         >  2:3137605/1‑148 (MQ=255)
                                                                                                        cTGTTCGACATGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttgt                                      <  1:2202123/148‑1 (MQ=255)
                                                                                                        cTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGtgttgt                                      >  2:1256032/1‑148 (MQ=255)
                                                                                                           ttCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGc                                   >  1:442640/1‑148 (MQ=255)
                                                                                                           ttCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGc                                   >  2:2607054/1‑148 (MQ=255)
                                                                                                                 aaGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGgcgc                             <  2:3452641/148‑1 (MQ=255)
                                                                                                                    gTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCggg                          >  2:1982826/1‑148 (MQ=255)
                                                                                                                       gCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaaa                       <  2:1690278/148‑1 (MQ=255)
                                                                                                                       gCAATCCAATAAAGTTGGCACTGGAAGAAACCTGCGGTTTACAATAAGCCTGGCAAAAAGAAACCACCACGGGATGGTCGGTTACTCGTACACCAAGCGAATAAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGaaa                       <  1:1861568/148‑1 (MQ=255)
                                                                                                                        cAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAg                      >  1:2420668/1‑148 (MQ=255)
                                                                                                                            ccACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACaa                  >  1:472766/1‑148 (MQ=255)
                                                                                                                             cACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACaaa                 <  2:1333097/148‑1 (MQ=255)
                                                                                                                               cTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAgg               >  1:2713362/1‑148 (MQ=255)
                                                                                                                                tCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGt              >  2:813058/1‑148 (MQ=255)
                                                                                                                                 cAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTg             >  2:1177725/1‑148 (MQ=255)
                                                                                                                                    gTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACa          >  2:769699/1‑148 (MQ=255)
                                                                                                                                    gTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACa          >  2:2156400/1‑148 (MQ=255)
                                                                                                                                    gTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACa          >  2:387039/1‑148 (MQ=255)
                                                                                                                                      tGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAgg        <  1:2547319/148‑1 (MQ=255)
                                                                                                                                      tGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAgg        <  1:3090088/148‑1 (MQ=255)
                                                                                                                                        gCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAc      <  1:447826/148‑1 (MQ=255)
                                                                                                                                        gCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAc      <  2:3215095/148‑1 (MQ=255)
                                                                                                                                         cACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGACAGACAAAGGTGACAGGAcc     >  2:2272313/1‑148 (MQ=255)
                                                                                                                                         cACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAcc     <  2:560146/148‑1 (MQ=255)
                                                                                                                                          aCTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCt    <  2:563015/148‑1 (MQ=255)
                                                                                                                                            tGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTgg  <  2:2335927/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGG  >  CP009273/3424220‑3424507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCAG  >  CP009273/3424214‑3424511
                                                                                                                                                     |                                                                                                                                                    
AAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGA                                                                                                                                                      >  SRR19973065.1086919/1‑150 (MQ=60)
   CAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGTATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGA                                                                                                                                                   <  SRR19973065.3196264/150‑1 (MQ=60)
   CAGTACACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGA                                                                                                                                                   <  SRR19973065.945792/150‑1 (MQ=60)
    AGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAA                                                                                                                                                  >  SRR19973065.1196605/1‑150 (MQ=60)
    AGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAA                                                                                                                                                  >  SRR19973065.3272666/1‑150 (MQ=60)
        CACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCA                                                                                                                                              >  SRR19973065.1451691/1‑150 (MQ=60)
          CCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGC                                                                                                                                            >  SRR19973065.1341921/1‑150 (MQ=60)
           CCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCG                                                                                                                                           <  SRR19973065.3138474/150‑1 (MQ=60)
           CCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCG                                                                                                                                           <  SRR19973065.1380911/150‑1 (MQ=60)
           CCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCG                                                                                                                                           <  SRR19973065.1380594/150‑1 (MQ=60)
           CCGACAGGGCATCGCGTATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCG                                                                                                                                           <  SRR19973065.59462/150‑1 (MQ=60)
             GTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGT                                                                                                                                         >  SRR19973065.3234482/1‑150 (MQ=60)
             GTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGT                                                                                                                                         >  SRR19973065.3234430/1‑150 (MQ=60)
             GTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGT                                                                                                                                         >  SRR19973065.2228049/1‑150 (MQ=60)
              TCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTT                                                                                                                                        <  SRR19973065.205800/150‑1 (MQ=60)
                AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                      >  SRR19973065.3440377/1‑150 (MQ=60)
                AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                      <  SRR19973065.3489767/150‑1 (MQ=60)
                AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                      <  SRR19973065.2351324/150‑1 (MQ=60)
                AGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTA                                                                                                                                      >  SRR19973065.397007/1‑150 (MQ=60)
                     ATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATA                                                                                                                                 >  SRR19973065.43075/1‑150 (MQ=60)
                       CGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAG                                                                                                                               <  SRR19973065.2157035/150‑1 (MQ=60)
                         CGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCC                                                                                                                             >  SRR19973065.2956960/1‑150 (MQ=60)
                          GGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCT                                                                                                                            <  SRR19973065.3520194/150‑1 (MQ=60)
                          GGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCT                                                                                                                            >  SRR19973065.1525368/1‑150 (MQ=60)
                          GGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCA                                                                                                                            >  SRR19973065.3141183/1‑150 (MQ=60)
                          GGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTAGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCT                                                                                                                            >  SRR19973065.1525541/1‑150 (MQ=60)
                           GATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTG                                                                                                                           <  SRR19973065.562468/150‑1 (MQ=60)
                              TTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCA                                                                                                                        >  SRR19973065.1301590/1‑150 (MQ=60)
                               TCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCAC                                                                                                                       <  SRR19973065.1173799/150‑1 (MQ=60)
                                CTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACA                                                                                                                      <  SRR19973065.3284145/150‑1 (MQ=60)
                                  GAATGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAATTTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAA                                                                                                                    <  SRR19973065.3410152/150‑1 (MQ=60)
                                  GAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAA                                                                                                                    <  SRR19973065.2475731/150‑1 (MQ=60)
                                  GAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAA                                                                                                                    <  SRR19973065.2475973/150‑1 (MQ=60)
                                   ACGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAG                                                                                                                   <  SRR19973065.646286/150‑1 (MQ=60)
                                   AAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAG                                                                                                                   <  SRR19973065.2279477/150‑1 (MQ=60)
                                     GGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCA                                                                                                                 >  SRR19973065.3024115/1‑150 (MQ=60)
                                     GGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACACAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCA                                                                                                                 >  SRR19973065.3024076/1‑150 (MQ=60)
                                        TTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACC                                                                                                              <  SRR19973065.2349540/150‑1 (MQ=60)
                                         TTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCA                                                                                                             >  SRR19973065.3464792/1‑150 (MQ=60)
                                         TTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCA                                                                                                             >  SRR19973065.2954073/1‑150 (MQ=60)
                                         TTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCA                                                                                                             >  SRR19973065.2954130/1‑150 (MQ=60)
                                           AAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACC                                                                                                           >  SRR19973065.466753/1‑150 (MQ=60)
                                           AAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACC                                                                                                           >  SRR19973065.1217148/1‑150 (MQ=60)
                                           AAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACC                                                                                                           >  SRR19973065.1217109/1‑150 (MQ=60)
                                               GCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACG                                                                                                       <  SRR19973065.3489905/150‑1 (MQ=60)
                                                     CCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGT                                                                                                 >  SRR19973065.315592/1‑150 (MQ=60)
                                                       GTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCG                                                                                               >  SRR19973065.3155287/1‑150 (MQ=60)
                                                       GTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCG                                                                                               >  SRR19973065.3154959/1‑150 (MQ=60)
                                                       GTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCG                                                                                               >  SRR19973065.1185014/1‑150 (MQ=60)
                                                         TTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGT                                                                                             >  SRR19973065.595111/1‑150 (MQ=60)
                                                           CACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGA                                                                                           >  SRR19973065.2071950/1‑150 (MQ=60)
                                                           CACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGAGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGA                                                                                           >  SRR19973065.288084/1‑150 (MQ=60)
                                                                 GCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTA                                                                                     <  SRR19973065.898634/150‑1 (MQ=60)
                                                                       CCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAA                                                                               >  SRR19973065.1257646/1‑150 (MQ=60)
                                                                         GGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGC                                                                             >  SRR19973065.405308/1‑150 (MQ=60)
                                                                               GCCGCGCCAAATTGTGCGCGAACTTAGTATACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCA                                                                       <  SRR19973065.2923352/150‑1 (MQ=60)
                                                                                 CGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAA                                                                     >  SRR19973065.839209/1‑150 (MQ=60)
                                                                                   CGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGC                                                                   >  SRR19973065.425556/1‑150 (MQ=60)
                                                                                    GCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCG                                                                  <  SRR19973065.302548/150‑1 (MQ=60)
                                                                                    GCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCG                                                                  <  SRR19973065.302439/150‑1 (MQ=60)
                                                                                       AAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCC                                                               >  SRR19973065.1697173/1‑150 (MQ=60)
                                                                                       AAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCC                                                               >  SRR19973065.1696220/1‑150 (MQ=60)
                                                                                               CGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACC                                                       >  SRR19973065.2350514/1‑150 (MQ=60)
                                                                                                   AACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCG                                                   >  SRR19973065.3449907/1‑150 (MQ=60)
                                                                                                        CGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTG                                              >  SRR19973065.144338/1‑150 (MQ=60)
                                                                                                         GTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGT                                             >  SRR19973065.483751/1‑150 (MQ=60)
                                                                                                          TCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTT                                            <  SRR19973065.1422927/150‑1 (MQ=60)
                                                                                                          TCTACTGTTCGAAATGGTGGCAATCCACTAAAGTTGGCACTGTAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGTTGGTCGGTGACTCGTACACCAAGCTAATCAAAGCGGCCCGTCAACCAGCGCGGTGTT                                            <  SRR19973065.2445186/150‑1 (MQ=60)
                                                                                                            TACTGTTCGACATGGTGGCAATCCACTCACGTTGGCACTGTAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGT                                          <  SRR19973065.1892548/150‑1 (MQ=60)
                                                                                                             ACTGTTCGACATGGGGGCAATCCACTCATGTTGGCACTGTAAGAAACCAGCGGTTTACAATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCACGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTC                                         <  SRR19973065.495405/150‑1 (MQ=60)
                                                                                                              CTGTTCGACATGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCG                                        <  SRR19973065.2216221/150‑1 (MQ=60)
                                                                                                               TGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGC                                       >  SRR19973065.445707/1‑150 (MQ=60)
                                                                                                                       AAGGTGGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGG                               >  SRR19973065.287536/1‑150 (MQ=60)
                                                                                                                            caaAATCCACTAATGTTGGCACTGGAAGAAACCTGGGGTTTACCATAAGCCTGTCAAAACCCAACCACCAACGGTTGGTCGGTGACTCGTACAGCAAGCGAATCAACGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAG                          <  SRR19973065.2162815/147‑1 (MQ=60)
                                                                                                                            GGCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAG                          >  SRR19973065.2435938/1‑150 (MQ=60)
                                                                                                                             GCAATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGA                         <  SRR19973065.1873783/150‑1 (MQ=60)
                                                                                                                             GCAATCCAATAAAGTTGGCACTGGAAGAAACCTGCGGTTTACAATAAGCCTGGCAAAAAGAAACCACCACGGGATGGTCGGTTACTCGTACACCAAGCGAATAAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGA                         <  SRR19973065.1873822/150‑1 (MQ=60)
                                                                                                                                ATCCACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAA                      >  SRR19973065.475999/1‑150 (MQ=60)
                                                                                                                                   CACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGG                   >  SRR19973065.2730234/1‑150 (MQ=60)
                                                                                                                                   CACTCAAGTTGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGG                   >  SRR19973065.435548/1‑150 (MQ=60)
                                                                                                                                            TGGCACTGTAAGAAACCAGCGGTTTACAATAAGCCTGGCACAACGCAACCACCAACGGTTGGTCGGTGACTCGTACAGAAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAC          <  SRR19973065.739384/150‑1 (MQ=60)
                                                                                                                                            TGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGNC          <  SRR19973065.2563270/150‑1 (MQ=60)
                                                                                                                                            TGGCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGAC          <  SRR19973065.3108952/150‑1 (MQ=60)
                                                                                                                                              GCACTGGAAGAACCCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCT        <  SRR19973065.450932/150‑1 (MQ=60)
                                                                                                                                              GCACTGGAAGAAACCAGCGTTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCTAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCT        <  SRR19973065.3116607/150‑1 (MQ=60)
                                                                                                                                              GCACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCT        <  SRR19973065.2583440/150‑1 (MQ=60)
                                                                                                                                               CACTGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCACCGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTG       <  SRR19973065.2215357/150‑1 (MQ=60)
                                                                                                                                                  TGGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCC    <  SRR19973065.2327572/150‑1 (MQ=60)
                                                                                                                                                   GGAAGAAACCAGCGGTTTACCATACGCCTGGCACAAAGCAACCACCACCGGATGGTCGGTGACTCGTACAGCACGCCAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCA   <  SRR19973065.1059511/150‑1 (MQ=60)
                                                                                                                                                   GGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCACCCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCA   <  SRR19973065.1145783/150‑1 (MQ=60)
                                                                                                                                                   GGAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCA   >  SRR19973065.2076577/1‑150 (MQ=60)
                                                                                                                                                    GAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCAG  >  SRR19973065.2374480/1‑150 (MQ=60)
                                                                                                                                                    GAAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCAG  <  SRR19973065.3236603/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTACCATAAGCCTGGCACAAAGCAACCACCAACGGATGGTCGGTGACTCGTACAGCAAGCGAATCAAAGCGGCCCGTCAACCAGCGCGGTGTTGTCGCAGGCGCGGGAAAGACAAAGGTGACAGGACCTGGCCAG  >  CP009273/3424214‑3424511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑