Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 652,866 Δ1 bp coding (119/210 nt) cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273652,8650G.98.9% 940.1 / 2.7 183coding (118/210 nt)cspEconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base . (96/85);  total (96/87)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 185 total aligned reads displayed.

TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010
                                                                                                                                                     |                                                                                                                                                 
ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATg                                                                                                                                                   <  1:789451/150‑1 (MQ=255)
    gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTACTGtttt                                                                                                                                               <  2:234873/150‑1 (MQ=255)
      cTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTaa                                                                                                                                            >  1:2837137/1‑150 (MQ=255)
          ttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAAct                                                                                                                                        <  2:1853676/150‑1 (MQ=255)
             tCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCTAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCtt                                                                                                                                     <  2:3496464/150‑1 (MQ=255)
              cATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTg                                                                                                                                    <  2:2067157/150‑1 (MQ=255)
                tGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCt                                                                                                                                  >  1:527423/1‑150 (MQ=255)
                  tAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGa                                                                                                                                <  2:3801216/150‑1 (MQ=255)
                  tAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGa                                                                                                                                >  2:2700775/1‑150 (MQ=255)
                   aaaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGaa                                                                                                                               >  2:2422282/1‑150 (MQ=255)
                    aaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAg                                                                                                                              >  1:1345835/1‑150 (MQ=255)
                    aaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAg                                                                                                                              >  2:3369857/1‑150 (MQ=255)
                     aGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAgg                                                                                                                             >  2:616034/1‑150 (MQ=255)
                       gTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTc                                                                                                                           <  1:2287453/150‑1 (MQ=255)
                       gTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTc                                                                                                                           <  1:2405604/150‑1 (MQ=255)
                        tAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCa                                                                                                                          >  1:2696254/1‑150 (MQ=255)
                        tAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCa                                                                                                                          >  2:1283534/1‑150 (MQ=255)
                            tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAgcgc                                                                                                                      >  2:2978085/1‑150 (MQ=255)
                             ttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGcgcg                                                                                                                     >  1:1308471/1‑150 (MQ=255)
                             ttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGcgcg                                                                                                                     >  1:3208821/1‑150 (MQ=255)
                                aTGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTag                                                                                                                  >  1:3245931/1‑150 (MQ=255)
                                 tGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTaga                                                                                                                 <  2:3307855/150‑1 (MQ=255)
                                  gTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTagag                                                                                                                <  2:965933/150‑1 (MQ=255)
                                  gTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTagag                                                                                                                >  2:1468949/1‑150 (MQ=255)
                                    cTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGtt                                                                                                              >  1:858815/1‑150 (MQ=255)
                                     tAAGATTAAAGTTAACGTTAAGTGGTTTAATGAGTCAAAAGGATTCGGTTTCATTACTCCGGTTGACGGCAGCAAAGACGTGTTCGTTAACTTCTCTGCAATACAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTc                                                                                                             <  1:311156/150‑1 (MQ=255)
                                     tAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTc                                                                                                             <  1:311109/150‑1 (MQ=255)
                                      aaGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCg                                                                                                            >  2:1101468/1‑150 (MQ=255)
                                         aTTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGaaa                                                                                                         >  2:2889646/1‑150 (MQ=255)
                                          ttAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAAt                                                                                                        <  2:1176242/150‑1 (MQ=255)
                                             aaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCAc                                                                                                     >  2:2109712/1‑150 (MQ=255)
                                              aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                                                    <  1:2103984/150‑1 (MQ=255)
                                               ggTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTa                                                                                                   <  1:1130960/150‑1 (MQ=255)
                                                 tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                                                                 <  1:1544502/150‑1 (MQ=255)
                                                 tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                                                                 >  1:1640344/1‑150 (MQ=255)
                                                 tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                                                                 >  1:3620719/1‑150 (MQ=255)
                                                    cGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAATCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGt                                                                                              >  2:2130860/1‑150 (MQ=255)
                                                      ttAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGc                                                                                            <  1:2873644/150‑1 (MQ=255)
                                                        aaGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCa                                                                                          <  1:2132649/150‑1 (MQ=255)
                                                          gTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCaaa                                                                                        >  1:2862030/1‑150 (MQ=255)
                                                            ggTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAgg                                                                                      <  2:629678/150‑1 (MQ=255)
                                                                  aTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTc                                                                                <  1:3382714/150‑1 (MQ=255)
                                                                  aTGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTc                                                                                <  1:1248846/150‑1 (MQ=255)
                                                                   tGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTct                                                                               <  2:1796294/150‑1 (MQ=255)
                                                                     aGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgc                                                                             >  1:3154857/1‑150 (MQ=255)
                                                                         cAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCa                                                                         <  2:233677/150‑1 (MQ=255)
                                                                           aaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaaa                                                                       >  2:2283721/1‑150 (MQ=255)
                                                                            aGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAAc                                                                      <  2:1334234/150‑1 (MQ=255)
                                                                              gATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGt                                                                    >  2:3178642/1‑150 (MQ=255)
                                                                                   ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                                                               >  1:838732/1‑150 (MQ=255)
                                                                                   ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                                                               <  1:640641/150‑1 (MQ=255)
                                                                                     tttCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGct                                                             <  2:2490968/150‑1 (MQ=255)
                                                                                       tCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGctct                                                           >  1:188137/1‑150 (MQ=255)
                                                                                         aTTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGt                                                         >  2:1713447/1‑150 (MQ=255)
                                                                                          ttACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTa                                                        >  1:2827175/1‑150 (MQ=255)
                                                                                           tACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTaa                                                       <  2:2837137/150‑1 (MQ=255)
                                                                                            aCTCCGGAAGACGGCAGCGAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAg                                                      >  2:72450/1‑150 (MQ=255)
                                                                                            aCTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAg                                                      >  1:754849/1‑150 (MQ=255)
                                                                                                  gAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGt                                                >  1:2496085/1‑150 (MQ=255)
                                                                                                   aaGACGGCATCAAAGACGTGTTCGTACTCTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGATGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCTAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGtg                                               <  2:425720/150‑2 (MQ=255)
                                                                                                   aaGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTc                                               >  2:2152561/1‑150 (MQ=255)
                                                                                                   aaGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTc                                               <  1:2223999/150‑1 (MQ=255)
                                                                                                    aGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCa                                              >  2:777886/1‑150 (MQ=255)
                                                                                                     gACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAg                                             <  1:2011068/150‑1 (MQ=255)
                                                                                                     gACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGCTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTACTCGCTCTGTAAGATACGTCAg                                             >  2:2463004/1‑150 (MQ=255)
                                                                                                       cGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCCGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCa                                           >  1:1359037/1‑150 (MQ=255)
                                                                                                        ggCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCaa                                          <  1:2377319/150‑1 (MQ=255)
                                                                                                        ggCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCaa                                          >  2:1005435/1‑150 (MQ=255)
                                                                                                           agcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                       <  2:2963172/150‑1 (MQ=255)
                                                                                                           agcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                       >  1:1807276/1‑150 (MQ=255)
                                                                                                           agcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGaa                                       >  2:2584379/1‑150 (MQ=255)
                                                                                                            gcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAt                                      <  2:3029160/150‑1 (MQ=255)
                                                                                                             caAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt                                     >  2:548595/1‑150 (MQ=255)
                                                                                                              aaaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtta                                    <  2:3041568/150‑2 (MQ=255)
                                                                                                              aaaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTc                                    >  1:1338138/1‑150 (MQ=255)
                                                                                                              aaaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTc                                    >  1:1600732/1‑150 (MQ=255)
                                                                                                               aaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCa                                   >  2:3223468/1‑150 (MQ=255)
                                                                                                                 gACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCaaa                                 >  2:3754524/1‑150 (MQ=255)
                                                                                                                   cGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCaaacc                               >  2:2247817/1‑148 (MQ=255)
                                                                                                                    gtgtTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAcc                              <  1:2681743/150‑1 (MQ=255)
                                                                                                                     tgtTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAAccc                             <  2:1511520/150‑1 (MQ=255)
                                                                                                                        tCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCt                          <  1:1476868/150‑1 (MQ=255)
                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                         <  1:3584209/150‑1 (MQ=255)
                                                                                                                         cGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt                         <  1:739875/150‑1 (MQ=255)
                                                                                                                           tACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTaa                       >  1:160528/1‑150 (MQ=255)
                                                                                                                            acacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                      <  1:2116179/150‑1 (MQ=255)
                                                                                                                            acacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                      >  2:1084529/1‑150 (MQ=255)
                                                                                                                             cacTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc                     >  2:1246874/1‑150 (MQ=255)
                                                                                                                              acTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                    >  2:760651/1‑150 (MQ=255)
                                                                                                                                ttCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc                  <  1:718747/150‑1 (MQ=255)
                                                                                                                                      gCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttt            <  1:3369857/150‑1 (MQ=255)
                                                                                                                                       cAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt           >  1:3588388/1‑150 (MQ=255)
                                                                                                                                       cAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt           <  1:1283531/150‑1 (MQ=255)
                                                                                                                                        aaTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt          >  1:1083355/1‑150 (MQ=255)
                                                                                                                                        aaTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt          >  1:1854821/1‑150 (MQ=255)
                                                                                                                                         aTCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGATGCAAACATAATCGCTCTATAAGATACGTCAACAATAATTCAAAACCCAATTAATCAACAGGtttttt         >  1:498400/1‑150 (MQ=255)
                                                                                                                                          tCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttttt        <  2:527423/150‑1 (MQ=255)
                                                                                                                                            cAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg      >  1:178631/1‑150 (MQ=255)
                                                                                                                                              gACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGt    <  1:2953557/150‑1 (MQ=255)
                                                                                                                                               aCTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc   >  2:218418/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCT  >  CP009273/652716‑653010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG  >  CP009273/652719‑653014
                                                                                                                                                   |                                                                                                                                                    
TGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGTTT                                                                                                                                                    <  SRR19973061.1265885/150‑1 (MQ=60)
   CTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAA                                                                                                                                                >  SRR19973061.2844403/1‑150 (MQ=60)
         TTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCT                                                                                                                                          <  SRR19973061.3317967/150‑1 (MQ=60)
             TGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCT                                                                                                                                      <  SRR19973061.851370/150‑1 (MQ=60)
             TGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCT                                                                                                                                      >  SRR19973061.528818/1‑150 (MQ=60)
             TGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGAT                                                                                                                                      <  SRR19973061.1502587/150‑1 (MQ=60)
                 AAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAG                                                                                                                                  >  SRR19973061.1349220/1‑150 (MQ=60)
                    GTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTC                                                                                                                               <  SRR19973061.2293237/150‑1 (MQ=60)
                    GTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTC                                                                                                                               <  SRR19973061.2411742/150‑1 (MQ=60)
                    GTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTC                                                                                                                               <  SRR19973061.2292781/150‑1 (MQ=60)
                    GTAATTTTGATGCCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTC                                                                                                                               <  SRR19973061.500466/150‑1 (MQ=60)
                     TAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCA                                                                                                                              >  SRR19973061.2703181/1‑150 (MQ=60)
                      AATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAG                                                                                                                             <  SRR19973061.1627478/150‑1 (MQ=60)
                       ATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGC                                                                                                                            >  SRR19973061.46126/1‑150 (MQ=60)
                         TTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGC                                                                                                                          >  SRR19973061.682274/1‑150 (MQ=60)
                          TTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCG                                                                                                                         >  SRR19973061.1311742/1‑150 (MQ=60)
                          TTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCG                                                                                                                         >  SRR19973061.3217068/1‑150 (MQ=60)
                             ATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAG                                                                                                                      <  SRR19973061.2859703/150‑1 (MQ=60)
                             ATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAG                                                                                                                      >  SRR19973061.3254282/1‑150 (MQ=60)
                             ATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAG                                                                                                                      >  SRR19973061.3409601/1‑150 (MQ=60)
                               GTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAG                                                                                                                    >  SRR19973061.3049434/1‑150 (MQ=60)
                                 CTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTT                                                                                                                  >  SRR19973061.861044/1‑150 (MQ=60)
                                  TAAGATTAAAGTTAACGTTAAGTGGTTTAATGAGTCAAAAGGATTCGGTTTCATTACTCCGGTTGACGGCAGCAAAGACGTGTTCGTTAACTTCTCTGCAATACAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTC                                                                                                                 <  SRR19973061.311973/150‑1 (MQ=60)
                                  TAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTC                                                                                                                 <  SRR19973061.311925/150‑1 (MQ=60)
                                   AAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCG                                                                                                                <  SRR19973061.1605504/150‑1 (MQ=60)
                                      ATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAA                                                                                                             >  SRR19973061.1148443/1‑150 (MQ=60)
                                           AGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACT                                                                                                        <  SRR19973061.2109268/150‑1 (MQ=60)
                                            GGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTA                                                                                                       <  SRR19973061.1133845/150‑1 (MQ=60)
                                              TAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAC                                                                                                     >  SRR19973061.1644453/1‑150 (MQ=60)
                                              TAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAC                                                                                                     <  SRR19973061.1548347/150‑1 (MQ=60)
                                              TAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAC                                                                                                     >  SRR19973061.3629984/1‑150 (MQ=60)
                                                   TTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGC                                                                                                <  SRR19973061.2880810/150‑1 (MQ=60)
                                                   TTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGC                                                                                                <  SRR19973061.2881011/150‑1 (MQ=60)
                                                    TAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCC                                                                                               >  SRR19973061.3176206/1‑150 (MQ=60)
                                                    TAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCC                                                                                               >  SRR19973061.2627763/1‑150 (MQ=60)
                                                     AAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCA                                                                                              <  SRR19973061.2138007/150‑1 (MQ=60)
                                                       GTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAA                                                                                            >  SRR19973061.2869359/1‑150 (MQ=60)
                                                               ATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTC                                                                                    <  SRR19973061.1251941/150‑1 (MQ=60)
                                                               ATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTC                                                                                    <  SRR19973061.3391405/150‑1 (MQ=60)
                                                                  AGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGC                                                                                 >  SRR19973061.3162965/1‑150 (MQ=60)
                                                                       AAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAA                                                                            >  SRR19973061.1577853/1‑150 (MQ=60)
                                                                                GGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCG                                                                   <  SRR19973061.642274/150‑1 (MQ=60)
                                                                                GGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCG                                                                   >  SRR19973061.290689/1‑150 (MQ=60)
                                                                                GGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCG                                                                   >  SRR19973061.840913/1‑150 (MQ=60)
                                                                                 GTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCATGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGC                                                                  >  SRR19973061.3128683/1‑150 (MQ=60)
                                                                                  TTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCT                                                                 <  SRR19973061.2834728/150‑1 (MQ=60)
                                                                                   TTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAGCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTC                                                                >  SRR19973061.188407/1‑150 (MQ=60)
                                                                                    TCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCT                                                               >  SRR19973061.188707/1‑150 (MQ=60)
                                                                                       TTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTA                                                            >  SRR19973061.2834417/1‑150 (MQ=60)
                                                                                        TACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAA                                                           >  SRR19973061.2126053/1‑150 (MQ=60)
                                                                                         ACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAG                                                          >  SRR19973061.756834/1‑150 (MQ=60)
                                                                                           TCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGAT                                                        <  SRR19973061.2428466/150‑1 (MQ=60)
                                                                                               GAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGT                                                    >  SRR19973061.2502441/1‑150 (MQ=60)
                                                                                                AAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTC                                                   <  SRR19973061.2229577/150‑1 (MQ=60)
                                                                                                 AGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCA                                                  <  SRR19973061.1346992/150‑1 (MQ=60)
                                                                                                 AGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCA                                                  <  SRR19973061.1998641/150‑1 (MQ=60)
                                                                                                  GACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAG                                                 >  SRR19973061.98386/1‑150 (MQ=60)
                                                                                                  GACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAG                                                 <  SRR19973061.2016112/150‑1 (MQ=60)
                                                                                                    CGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCCGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCA                                               >  SRR19973061.1362461/1‑150 (MQ=60)
                                                                                                     GGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAA                                              <  SRR19973061.2383373/150‑1 (MQ=60)
                                                                                                      GCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAG                                             <  SRR19973061.1848173/150‑1 (MQ=60)
                                                                                                        AGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAA                                           >  SRR19973061.1811789/1‑150 (MQ=60)
                                                                                                         GCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAT                                          <  SRR19973061.1776107/150‑1 (MQ=60)
                                                                                                          CAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATT                                         >  SRR19973061.795051/1‑150 (MQ=60)
                                                                                                           AAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTC                                        >  SRR19973061.1340478/1‑150 (MQ=60)
                                                                                                           AAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTC                                        >  SRR19973061.1341504/1‑150 (MQ=60)
                                                                                                           AAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTC                                        >  SRR19973061.1604709/1‑150 (MQ=60)
                                                                                                                 GTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACC                                  <  SRR19973061.2688649/150‑1 (MQ=60)
                                                                                                                 GTGTTCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACC                                  <  SRR19973061.779670/150‑1 (MQ=60)
                                                                                                                     TCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCT                              <  SRR19973061.2707718/150‑1 (MQ=60)
                                                                                                                     TCGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCT                              <  SRR19973061.1480574/150‑1 (MQ=60)
                                                                                                                      CGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGACTTCAAAACCCGCTT                             >  SRR19973061.2052148/1‑150 (MQ=60)
                                                                                                                      CGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT                             <  SRR19973061.741814/150‑1 (MQ=60)
                                                                                                                      CGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT                             <  SRR19973061.741978/150‑1 (MQ=60)
                                                                                                                      CGTACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTT                             <  SRR19973061.3593400/150‑1 (MQ=60)
                                                                                                                        TACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAA                           >  SRR19973061.161029/1‑150 (MQ=60)
                                                                                                                         ACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAT                          <  SRR19973061.2121494/150‑1 (MQ=60)
                                                                                                                         ACACTTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAT                          <  SRR19973061.2120843/150‑1 (MQ=60)
                                                                                                                             TTCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGC                      <  SRR19973061.720619/150‑1 (MQ=60)
                                                                                                                              TCTCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAGGAATTCAAAACCCGCTTAATCAGCG                     <  SRR19973061.2388963/150‑1 (MQ=60)
                                                                                                                                TCTGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG                   <  SRR19973061.2348368/150‑1 (MQ=60)
                                                                                                                                  TGCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTT                 <  SRR19973061.1452198/150‑1 (MQ=60)
                                                                                                                                   GCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTT                <  SRR19973061.3386757/150‑1 (MQ=60)
                                                                                                                                   GCAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTT                <  SRR19973061.3378520/150‑1 (MQ=60)
                                                                                                                                    CAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTT               <  SRR19973061.1286707/150‑1 (MQ=60)
                                                                                                                                    CAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTT               >  SRR19973061.3597587/1‑150 (MQ=60)
                                                                                                                                    CAATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTT               <  SRR19973061.1286710/150‑1 (MQ=60)
                                                                                                                                     AATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTT              >  SRR19973061.1086134/1‑150 (MQ=60)
                                                                                                                                     AATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTT              >  SRR19973061.3252842/1‑150 (MQ=60)
                                                                                                                                     AATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTT              >  SRR19973061.1859459/1‑150 (MQ=60)
                                                                                                                                     AATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTT              <  SRR19973061.617615/150‑1 (MQ=60)
                                                                                                                                      ATCCAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGATGCAAACATAATCGCTCTATAAGATACGTCAACAATAATTCAAAACCCAATTAATCAACAGGTTTTTT             >  SRR19973061.499716/1‑150 (MQ=60)
                                                                                                                                         CAGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTG          >  SRR19973061.179174/1‑150 (MQ=60)
                                                                                                                                          AGACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGG         >  SRR19973061.3612426/1‑150 (MQ=60)
                                                                                                                                           GACTAAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGT        <  SRR19973061.2961175/150‑1 (MQ=60)
                                                                                                                                               AAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTT    >  SRR19973061.1891733/1‑150 (MQ=60)
                                                                                                                                               AAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTT    <  SRR19973061.2115034/150‑1 (MQ=60)
                                                                                                                                               AAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTT    <  SRR19973061.2115006/150‑1 (MQ=60)
                                                                                                                                               AAT‑GTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTT    >  SRR19973061.983025/1‑150 (MQ=60)
                                                                                                                                                 atGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTA   >  SRR19973061.3814918/3‑150 (MQ=60)
                                                                                                                                                  TGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG  <  SRR19973061.263675/150‑1 (MQ=60)
                                                                                                                                                  TGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG  <  SRR19973061.1444398/150‑1 (MQ=60)
                                                                                                                                                  TGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG  <  SRR19973061.3348244/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
TGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG  >  CP009273/652719‑653014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑