Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 667,899 C→A D781Y (GAT→TAT)  leuS ← leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,8990CA100.0% 600.5 / NA 181D781Y (GAT→TAT) leuSleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (77/104);  total (77/104)

Only 100 of 203 total aligned reads displayed.

CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCA  >  CP009273/667752‑668045
                                                                                                                                                   |                                                                                                                                                  
cAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagc                                                                                                                                                  >  1:1847012/1‑150 (MQ=255)
cAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagc                                                                                                                                                  >  1:2264729/1‑150 (MQ=255)
cAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagc                                                                                                                                                  >  1:3724968/1‑150 (MQ=255)
 aGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcc                                                                                                                                                 >  1:1805179/1‑150 (MQ=255)
  gCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcct                                                                                                                                                <  1:2061178/150‑1 (MQ=255)
  gCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGACCACGACGCGTTGTCGATAtagcct                                                                                                                                                >  1:3330016/1‑150 (MQ=255)
   cACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcctt                                                                                                                                               <  2:2335360/150‑1 (MQ=255)
   cACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcctt                                                                                                                                               <  2:297995/150‑1 (MQ=255)
    aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttc                                                                                                                                              <  1:673990/150‑1 (MQ=255)
    aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttc                                                                                                                                              <  2:3287196/150‑1 (MQ=255)
     cGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttcg                                                                                                                                             >  2:1987547/1‑150 (MQ=255)
       ttCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttcgcc                                                                                                                                           <  2:2165235/150‑1 (MQ=255)
          gcgAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCttt                                                                                                                                        >  1:2718715/1‑150 (MQ=255)
            gAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                      <  1:912233/150‑1 (MQ=255)
             aaCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAg                                                                                                                                     <  2:395253/150‑1 (MQ=255)
              aCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGt                                                                                                                                    <  1:2232895/150‑1 (MQ=255)
              aCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGt                                                                                                                                    >  1:1317508/1‑150 (MQ=255)
               ccTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGtt                                                                                                                                   <  1:3704168/150‑1 (MQ=255)
                cTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTc                                                                                                                                  <  1:2248916/150‑1 (MQ=255)
                  gTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCt                                                                                                                                >  1:1765757/1‑150 (MQ=255)
                   ttcttcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTg                                                                                                                               >  1:3059613/1‑150 (MQ=255)
                      ttcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCca                                                                                                                            <  2:2264729/150‑1 (MQ=255)
                          gTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGc                                                                                                                        <  2:1161834/150‑1 (MQ=255)
                          gTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGc                                                                                                                        >  1:465610/1‑150 (MQ=255)
                            tGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGt                                                                                                                      >  1:3654/1‑150 (MQ=255)
                              cGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGa                                                                                                                    >  1:620320/1‑150 (MQ=255)
                               gTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGaa                                                                                                                   >  2:3560871/1‑150 (MQ=255)
                                  cACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                                <  2:3149806/150‑1 (MQ=255)
                                  cACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                                <  1:2554104/150‑1 (MQ=255)
                                  cACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCCGCCACAGCGTGAAGCa                                                                                                                <  1:645525/150‑1 (MQ=255)
                                      ggAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCTTGAAGCAGAtg                                                                                                            >  1:2537482/1‑150 (MQ=255)
                                      ggAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                            >  2:1630738/1‑150 (MQ=255)
                                       gAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgt                                                                                                           >  2:2820249/1‑150 (MQ=255)
                                        aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgtg                                                                                                          <  1:2776376/150‑1 (MQ=255)
                                         aCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGc                                                                                                         <  1:2415153/150‑1 (MQ=255)
                                             tGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgggg                                                                                                     <  2:2105017/150‑1 (MQ=255)
                                             tGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgggg                                                                                                     >  1:2478897/1‑150 (MQ=255)
                                              gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                    >  2:613075/1‑150 (MQ=255)
                                                 tttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGaa                                                                                                 >  1:2384710/1‑150 (MQ=255)
                                                  tgggCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAAc                                                                                                <  1:3349436/148‑1 (MQ=255)
                                                     gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACggg                                                                                             <  2:881788/150‑1 (MQ=255)
                                                     gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACggg                                                                                             <  2:130643/150‑1 (MQ=255)
                                                       aCGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGtt                                                                                           <  2:3353188/150‑1 (MQ=255)
                                                        cGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTa                                                                                          <  2:1207328/150‑1 (MQ=255)
                                                         ggACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTaa                                                                                         >  2:102469/1‑150 (MQ=255)
                                                          gACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAg                                                                                        <  1:2395761/150‑1 (MQ=255)
                                                          gACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAg                                                                                        >  2:740064/1‑150 (MQ=255)
                                                           aCTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGc                                                                                       >  1:1313898/1‑150 (MQ=255)
                                                               tACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAc                                                                                   <  1:1891314/150‑1 (MQ=255)
                                                               tACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAc                                                                                   <  1:732749/150‑1 (MQ=255)
                                                               tACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAc                                                                                   <  1:2636503/150‑1 (MQ=255)
                                                                aCCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGACCGGGTTAAGCATACg                                                                                  >  2:3045782/1‑150 (MQ=255)
                                                                aCCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACg                                                                                  >  2:2299417/1‑150 (MQ=255)
                                                                  cGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGa                                                                                <  2:1054624/150‑1 (MQ=255)
                                                                   gTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAc                                                                               <  1:2625935/150‑1 (MQ=255)
                                                                      aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAc                                                                            >  1:153587/1‑150 (MQ=255)
                                                                       aCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACg                                                                           <  1:20983/150‑1 (MQ=255)
                                                                       aCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACg                                                                           <  1:214148/150‑1 (MQ=255)
                                                                          tGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGcc                                                                        >  1:3411653/1‑150 (MQ=255)
                                                                            caccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcag                                                                      >  2:891005/1‑150 (MQ=255)
                                                                            caccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcag                                                                      >  2:285597/1‑150 (MQ=255)
                                                                            caccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcag                                                                      <  1:3505596/150‑1 (MQ=255)
                                                                               cacGACCAGCGTGGAGTCTTCCACCCTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGTGATAGCCTTCGCCTGTCAGGTCCTGCCACAGCGTGAAGCAGTTGTGCGGGGTGACCGGGTTAAGCATACGGACAACGGCcagcag                                                                   >  2:1898958/1‑150 (MQ=255)
                                                                                  gACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGc                                                                >  2:2757287/1‑150 (MQ=255)
                                                                                   aCCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCt                                                               <  2:346592/150‑1 (MQ=255)
                                                                                   aCCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCt                                                               <  2:3776169/150‑1 (MQ=255)
                                                                                        cGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTg                                                          >  2:1625274/1‑150 (MQ=255)
                                                                                           ggAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCAt                                                       <  2:3703102/150‑1 (MQ=255)
                                                                                           ggAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCAt                                                       <  2:1809350/150‑1 (MQ=255)
                                                                                             aGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCa                                                     >  2:3017867/1‑150 (MQ=255)
                                                                                             aGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCa                                                     <  1:2309827/150‑1 (MQ=255)
                                                                                              gTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCag                                                    >  2:3673465/1‑150 (MQ=255)
                                                                                               tCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCaga                                                   <  2:682669/150‑1 (MQ=255)
                                                                                                 ttCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAgc                                                 <  1:1987547/150‑1 (MQ=255)
                                                                                                   ccaccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAgcgc                                               >  1:580313/1‑150 (MQ=255)
                                                                                                    caccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGcgcg                                              <  1:3626744/150‑1 (MQ=255)
                                                                                                     accaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGa                                             >  1:3672586/1‑150 (MQ=255)
                                                                                                     accaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGa                                             >  1:1772318/1‑150 (MQ=255)
                                                                                                     accaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGa                                             <  2:3059613/150‑1 (MQ=255)
                                                                                                      ccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAt                                            <  1:139279/150‑1 (MQ=255)
                                                                                                       caccGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                                           <  1:1135354/147‑1 (MQ=255)
                                                                                                         tCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCt                                         <  1:2249672/150‑1 (MQ=255)
                                                                                                              ttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCg                                    >  2:1524252/1‑150 (MQ=255)
                                                                                                                ttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGcc                                  <  2:2007303/150‑1 (MQ=255)
                                                                                                                  cGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCAt                                <  2:2718715/150‑1 (MQ=255)
                                                                                                                    tCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCg                              <  1:974864/150‑1 (MQ=255)
                                                                                                                         aaCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                         >  2:1764491/1‑150 (MQ=255)
                                                                                                                                cACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTc                  <  1:2597389/150‑1 (MQ=255)
                                                                                                                                cACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTc                  <  1:3264343/150‑1 (MQ=255)
                                                                                                                                 aCGGCGCGTTGTCGATAGAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAGGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCg                 <  2:2485823/150‑1 (MQ=255)
                                                                                                                                   ggCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGcc               >  1:3765568/1‑150 (MQ=255)
                                                                                                                                    gcgcGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCa              <  2:3724968/150‑1 (MQ=255)
                                                                                                                                     cgcgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAg             >  1:421476/1‑150 (MQ=255)
                                                                                                                                        gTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGttt          <  1:1630738/150‑1 (MQ=255)
                                                                                                                                            tCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtc      <  1:2654752/150‑1 (MQ=255)
                                                                                                                                            tCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtc      >  1:547073/1‑150 (MQ=255)
                                                                                                                                              gATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcat    >  2:549774/1‑150 (MQ=255)
                                                                                                                                              gATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcat    <  2:1449413/150‑1 (MQ=255)
                                                                                                                                               atatAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatc   >  1:2792853/1‑150 (MQ=255)
                                                                                                                                                tataGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcatca  >  2:256335/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCA  >  CP009273/667752‑668045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAGC  >  CP009273/667751‑668047
                                                                                                                                                    |                                                                                                                                                    
CCAGCACGTTCGCGAACCTGTTCTTCCGGTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAG                                                                                                                                                     <  SRR19973061.3558718/150‑1 (MQ=60)
 CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGC                                                                                                                                                    >  SRR19973061.1851638/1‑150 (MQ=60)
 CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGC                                                                                                                                                    >  SRR19973061.2270443/1‑150 (MQ=60)
 CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGC                                                                                                                                                    >  SRR19973061.3705510/1‑150 (MQ=60)
 CAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGC                                                                                                                                                    >  SRR19973061.3734489/1‑150 (MQ=60)
  AGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCC                                                                                                                                                   >  SRR19973061.1809685/1‑150 (MQ=60)
   GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCT                                                                                                                                                  <  SRR19973061.2066364/150‑1 (MQ=60)
   GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGACCACGACGCGTTGTCGATATAGCCT                                                                                                                                                  >  SRR19973061.3338596/1‑150 (MQ=60)
     ACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTC                                                                                                                                                <  SRR19973061.675737/150‑1 (MQ=60)
      CGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCG                                                                                                                                               <  SRR19973061.3521613/150‑1 (MQ=60)
       GTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGC                                                                                                                                              >  SRR19973061.684440/1‑150 (MQ=60)
           GCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTT                                                                                                                                          <  SRR19973061.1160690/150‑1 (MQ=60)
           GCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTT                                                                                                                                          >  SRR19973061.2725713/1‑150 (MQ=60)
             GAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCA                                                                                                                                        <  SRR19973061.914572/150‑1 (MQ=60)
               ACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGT                                                                                                                                      <  SRR19973061.2238499/150‑1 (MQ=60)
               ACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGT                                                                                                                                      <  SRR19973061.434610/150‑1 (MQ=60)
               ACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGT                                                                                                                                      >  SRR19973061.1320818/1‑150 (MQ=60)
               ACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGT                                                                                                                                      <  SRR19973061.2733398/150‑1 (MQ=60)
                CCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTT                                                                                                                                     <  SRR19973061.3713636/150‑1 (MQ=60)
                 CTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTC                                                                                                                                    <  SRR19973061.2254574/150‑1 (MQ=60)
                   GTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCT                                                                                                                                  >  SRR19973061.484749/1‑150 (MQ=60)
                   GTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCT                                                                                                                                  >  SRR19973061.1770666/1‑150 (MQ=60)
                   GTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCT                                                                                                                                  >  SRR19973061.1770181/1‑150 (MQ=60)
                    TTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTG                                                                                                                                 <  SRR19973061.255007/150‑1 (MQ=60)
                    TTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTG                                                                                                                                 >  SRR19973061.3067515/1‑150 (MQ=60)
                          CGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAG                                                                                                                           >  SRR19973061.1367510/1‑150 (MQ=60)
                          CGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAG                                                                                                                           <  SRR19973061.2400520/150‑1 (MQ=60)
                           GTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGC                                                                                                                          >  SRR19973061.466874/1‑150 (MQ=60)
                           GTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGC                                                                                                                          >  SRR19973061.1318300/1‑150 (MQ=60)
                            TTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCG                                                                                                                         <  SRR19973061.3644491/150‑1 (MQ=60)
                             TGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCTCCTTTCAGTTCCTGCCACAGCGT                                                                                                                        >  SRR19973061.2653620/1‑150 (MQ=60)
                             TGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGT                                                                                                                        <  SRR19973061.3357685/150‑1 (MQ=60)
                             TGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGT                                                                                                                        >  SRR19973061.3662/1‑150 (MQ=60)
                              GCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTG                                                                                                                       <  SRR19973061.1860641/150‑1 (MQ=60)
                               CGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGA                                                                                                                      >  SRR19973061.2502028/1‑150 (MQ=60)
                               CGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGA                                                                                                                      >  SRR19973061.621904/1‑150 (MQ=60)
                                   CACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCA                                                                                                                  <  SRR19973061.2560614/150‑1 (MQ=60)
                                   CACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCCGCCACAGCGTGAAGCA                                                                                                                  <  SRR19973061.647179/150‑1 (MQ=60)
                                       GGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCTTGAAGCAGATG                                                                                                              >  SRR19973061.2543941/1‑150 (MQ=60)
                                       GGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATG                                                                                                              >  SRR19973061.633304/1‑150 (MQ=60)
                                         AACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTG                                                                                                            <  SRR19973061.1674863/150‑1 (MQ=60)
                                         AACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTG                                                                                                            <  SRR19973061.2783518/150‑1 (MQ=60)
                                         AACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTG                                                                                                            <  SRR19973061.2783619/150‑1 (MQ=60)
                                          ACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGC                                                                                                           <  SRR19973061.2421318/150‑1 (MQ=60)
                                            GGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCGTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGG                                                                                                         <  SRR19973061.943897/150‑1 (MQ=60)
                                             GTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGG                                                                                                        >  SRR19973061.1453072/1‑150 (MQ=60)
                                              TGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGG                                                                                                       >  SRR19973061.2485206/1‑150 (MQ=60)
                                                 TTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGA                                                                                                    <  SRR19973061.3132403/150‑1 (MQ=60)
                                                  TTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAA                                                                                                   >  SRR19973061.2390789/1‑150 (MQ=60)
                                                   TGGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAAC                                                                                                  <  SRR19973061.3358057/150‑1 (MQ=60)
                                                           GACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAG                                                                                          <  SRR19973061.2401869/150‑1 (MQ=60)
                                                           GACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAG                                                                                          >  SRR19973061.192164/1‑150 (MQ=60)
                                                            ACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGC                                                                                         >  SRR19973061.1317193/1‑150 (MQ=60)
                                                              TTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCAT                                                                                       <  SRR19973061.3016191/150‑1 (MQ=60)
                                                               TTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATA                                                                                      <  SRR19973061.2273948/150‑1 (MQ=60)
                                                                TACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAC                                                                                     <  SRR19973061.2643277/150‑1 (MQ=60)
                                                                TACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAC                                                                                     >  SRR19973061.3663970/1‑150 (MQ=60)
                                                                TACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAC                                                                                     <  SRR19973061.734669/150‑1 (MQ=60)
                                                                TACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAC                                                                                     <  SRR19973061.1896012/150‑1 (MQ=60)
                                                                    GTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAC                                                                                 <  SRR19973061.2632685/150‑1 (MQ=60)
                                                                       AACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAC                                                                              >  SRR19973061.244932/1‑150 (MQ=60)
                                                                       AACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAC                                                                              >  SRR19973061.154068/1‑150 (MQ=60)
                                                                        ACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACG                                                                             <  SRR19973061.214781/150‑1 (MQ=60)
                                                                        ACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACG                                                                             <  SRR19973061.21063/150‑1 (MQ=60)
                                                                           TGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCC                                                                          >  SRR19973061.3420414/1‑150 (MQ=60)
                                                                           TGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCC                                                                          <  SRR19973061.989139/150‑1 (MQ=60)
                                                                             CACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAG                                                                        <  SRR19973061.3514567/150‑1 (MQ=60)
                                                                                  CGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTG                                                                   <  SRR19973061.2071112/150‑1 (MQ=60)
                                                                                   GACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGC                                                                  <  SRR19973061.22614/150‑1 (MQ=60)
                                                                                   GACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGC                                                                  <  SRR19973061.3719135/150‑1 (MQ=60)
                                                                                   GACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGC                                                                  >  SRR19973061.653005/1‑150 (MQ=60)
                                                                                   GACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGC                                                                  <  SRR19973061.22449/150‑1 (MQ=60)
                                                                                          GTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGC                                                           <  SRR19973061.3418317/150‑1 (MQ=60)
                                                                                              AGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCA                                                       <  SRR19973061.2315685/150‑1 (MQ=60)
                                                                                                TCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGA                                                     >  SRR19973061.1785045/1‑150 (MQ=60)
                                                                                                  TTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGC                                                   <  SRR19973061.1992521/150‑1 (MQ=60)
                                                                                                    CCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGC                                                 >  SRR19973061.581826/1‑150 (MQ=60)
                                                                                                     CACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCG                                                <  SRR19973061.3636019/150‑1 (MQ=60)
                                                                                                      ACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGA                                               >  SRR19973061.1776757/1‑150 (MQ=60)
                                                                                                      ACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGA                                               >  SRR19973061.3681974/1‑150 (MQ=60)
                                                                                                       CCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAT                                              <  SRR19973061.139727/150‑1 (MQ=60)
                                                                                                        CATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATC                                             <  SRR19973061.591589/150‑1 (MQ=60)
                                                                                                        CACCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATC                                             <  SRR19973061.1138248/150‑1 (MQ=60)
                                                                                                         ATCGCTTTTTCGTCAGCAACCGGCCAC‑GCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCT                                           >  SRR19973061.2634469/1‑150 (MQ=60)
                                                                                                          TCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCT                                           <  SRR19973061.2255331/150‑1 (MQ=60)
                                                                                                          TCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCT                                           <  SRR19973061.2255068/150‑1 (MQ=60)
                                                                                                          TCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCT                                           <  SRR19973061.569686/150‑1 (MQ=60)
                                                                                                              TTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTC                                       >  SRR19973061.2155363/1‑150 (MQ=60)
                                                                                                              TTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTC                                       >  SRR19973061.211868/1‑150 (MQ=60)
                                                                                                               TTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCG                                      <  SRR19973061.157748/150‑1 (MQ=60)
                                                                                                                  TCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCA                                   <  SRR19973061.2060514/150‑1 (MQ=60)
                                                                                                                   CGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCAT                                  >  SRR19973061.3483488/1‑150 (MQ=60)
                                                                                                                     TCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCG                                <  SRR19973061.977346/150‑1 (MQ=60)
                                                                                                                      CAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGG                               <  SRR19973061.3279245/150‑1 (MQ=60)
                                                                                                                          AACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGG                           >  SRR19973061.3225876/1‑150 (MQ=60)
                                                                                                                           ACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGT                          <  SRR19973061.2695896/150‑1 (MQ=60)
                                                                                                                              GGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCT                       >  SRR19973061.2420326/1‑150 (MQ=60)
                                                                                                                              GGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCT                       <  SRR19973061.231854/150‑1 (MQ=60)
                                                                                                                               GCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTT                      <  SRR19973061.797224/150‑1 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                    <  SRR19973061.2604111/150‑1 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                    >  SRR19973061.3786066/1‑150 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                    >  SRR19973061.183186/1‑150 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                    <  SRR19973061.3272746/150‑1 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                    <  SRR19973061.2604054/150‑1 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                    <  SRR19973061.2603607/150‑1 (MQ=60)
                                                                                                                                    GGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCC                 >  SRR19973061.3775189/1‑150 (MQ=60)
                                                                                                                                      CGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAG               >  SRR19973061.422622/1‑150 (MQ=60)
                                                                                                                                         GTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTT            <  SRR19973061.1634815/150‑1 (MQ=60)
                                                                                                                                             TCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTC        >  SRR19973061.548513/1‑150 (MQ=60)
                                                                                                                                             TCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTC        <  SRR19973061.2661572/150‑1 (MQ=60)
                                                                                                                                                ATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATC     <  SRR19973061.3750858/150‑1 (MQ=60)
                                                                                                                                                ATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATC     >  SRR19973061.2800027/1‑150 (MQ=60)
                                                                                                                                                   TAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAGC  >  SRR19973061.2619218/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAGC  >  CP009273/667751‑668047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑