Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 667,899 C→A 100% D781Y (GAT→TAT)  leuS ← leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,8990CA99.0% 643.5 / ‑1.6 199D781Y (GAT→TAT) leuSleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (81/116);  total (83/116)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCAT  >  CP009273/667754‑668043
                                                                                                                                                 |                                                                                                                                                
 cACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcc                                                                                                                                               >  1:1748562/1‑148 (MQ=255)
  aCGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcct                                                                                                                                              <  2:2981889/148‑1 (MQ=255)
   cGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcctt                                                                                                                                             >  2:2440524/1‑148 (MQ=255)
      tCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttcgc                                                                                                                                          >  2:1580244/1‑148 (MQ=255)
           aaCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTc                                                                                                                                     >  1:1861871/1‑148 (MQ=255)
            aCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                    >  2:2919598/1‑148 (MQ=255)
              cTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGt                                                                                                                                  >  1:2711231/1‑148 (MQ=255)
               tGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGtt                                                                                                                                 >  2:261108/1‑148 (MQ=255)
                gTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTc                                                                                                                                <  2:2659493/148‑1 (MQ=255)
                 ttcttcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTcc                                                                                                                               >  1:489038/1‑148 (MQ=255)
                     tcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTAGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGcc                                                                                                                           >  1:2700724/1‑148 (MQ=255)
                         ttGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAg                                                                                                                       <  2:2650998/148‑1 (MQ=255)
                         ttGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAg                                                                                                                       <  2:2650808/148‑1 (MQ=255)
                          tGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGc                                                                                                                      >  1:1484161/1‑148 (MQ=255)
                           gCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCg                                                                                                                     >  2:2762962/1‑148 (MQ=255)
                           gCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCg                                                                                                                     <  2:1939962/148‑1 (MQ=255)
                                 aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                               >  2:1533468/1‑148 (MQ=255)
                                 aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                               <  1:1104706/148‑1 (MQ=255)
                                 aCCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGc                                                                                                               <  1:3419580/148‑1 (MQ=255)
                                  ccGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                              >  1:3224314/1‑148 (MQ=255)
                                  ccGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCa                                                                                                              >  1:1220280/1‑148 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  1:3167300/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  1:219658/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  1:1257992/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  2:3312573/148‑1 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          >  2:2459729/1‑148 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          >  1:2022408/1‑148 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          >  2:1228255/1‑148 (MQ=255)
                                        cGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgtg                                                                                                        >  1:2117056/1‑148 (MQ=255)
                                          gTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCg                                                                                                      <  2:3122466/148‑1 (MQ=255)
                                          gTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCg                                                                                                      <  2:1349116/148‑1 (MQ=255)
                                           tGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgg                                                                                                     <  2:252765/148‑1 (MQ=255)
                                           tGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgg                                                                                                     <  1:872940/148‑1 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    >  1:1564310/1‑148 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    >  2:2043412/1‑148 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    >  2:1722373/1‑148 (MQ=255)
                                              ttttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                  <  1:2752431/148‑1 (MQ=255)
                                              ttttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGt                                                                                                  >  1:138645/1‑148 (MQ=255)
                                                ttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGa                                                                                                <  2:37368/148‑1 (MQ=255)
                                                ttGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGa                                                                                                <  2:2341118/148‑1 (MQ=255)
                                                   gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACg                                                                                             >  2:3440256/1‑148 (MQ=255)
                                                   gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACg                                                                                             >  1:2387260/1‑148 (MQ=255)
                                                     aCGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACggg                                                                                           <  2:909174/148‑1 (MQ=255)
                                                      cGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGt                                                                                          <  1:1363397/148‑1 (MQ=255)
                                                          cTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAg                                                                                      <  2:1572117/148‑1 (MQ=255)
                                                           tttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGc                                                                                     <  2:489038/148‑1 (MQ=255)
                                                           tttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGc                                                                                     <  1:2575349/148‑1 (MQ=255)
                                                            ttACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCa                                                                                    >  1:2509550/1‑148 (MQ=255)
                                                              aCCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATa                                                                                  >  2:2809566/1‑148 (MQ=255)
                                                                cGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACg                                                                                >  1:3074661/1‑148 (MQ=255)
                                                                 gTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACgg                                                                               <  1:1891275/148‑1 (MQ=255)
                                                                 gTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACgg                                                                               <  1:2547920/148‑1 (MQ=255)
                                                                    aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACa                                                                            <  1:3270863/148‑1 (MQ=255)
                                                                     aCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACaa                                                                           >  1:3324663/1‑148 (MQ=255)
                                                                     aCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACaa                                                                           <  2:1416556/148‑1 (MQ=255)
                                                                      ccTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATTCGGACAAc                                                                          >  1:1530949/1‑148 (MQ=255)
                                                                        tGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACgg                                                                        >  2:827680/1‑148 (MQ=255)
                                                                        tGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACgg                                                                        >  1:709841/1‑148 (MQ=255)
                                                                         gCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGc                                                                       <  2:32278/148‑1 (MQ=255)
                                                                                  ccAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGc                                                              <  1:1580246/148‑1 (MQ=255)
                                                                                     gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                                           <  1:540142/148‑1 (MQ=255)
                                                                                      cGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTcc                                                          <  1:1638081/148‑1 (MQ=255)
                                                                                      cGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTcc                                                          >  1:1081267/1‑148 (MQ=255)
                                                                                          gAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCa                                                      >  1:909483/1‑148 (MQ=255)
                                                                                          gAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCa                                                      >  2:1554540/1‑148 (MQ=255)
                                                                                             tCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCa                                                   >  1:2776698/1‑148 (MQ=255)
                                                                                              cTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCag                                                  >  2:169240/1‑148 (MQ=255)
                                                                                                tCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCagag                                                <  1:753933/148‑1 (MQ=255)
                                                                                                    ccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGcgcg                                            >  2:400027/1‑148 (MQ=255)
                                                                                                     caTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCAACAGAGCGCGa                                           >  2:1615849/1‑148 (MQ=255)
                                                                                                      aTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAt                                          <  2:2143424/148‑1 (MQ=255)
                                                                                                      aTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAt                                          <  2:3148748/148‑1 (MQ=255)
                                                                                                       tCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                                         <  2:3321481/148‑1 (MQ=255)
                                                                                                       tCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATc                                         <  2:7640/148‑1 (MQ=255)
                                                                                                        cGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATcc                                        <  2:3100332/148‑1 (MQ=255)
                                                                                                           tttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGc                                     <  2:1372212/148‑1 (MQ=255)
                                                                                                            ttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCt                                    <  1:3503033/148‑1 (MQ=255)
                                                                                                                  tCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCAt                              <  1:2962450/148‑1 (MQ=255)
                                                                                                                   cAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATc                             >  1:580054/1‑148 (MQ=255)
                                                                                                                     gCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGNGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCgg                           <  1:1805435/148‑1 (MQ=255)
                                                                                                                     gCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCgg                           <  1:688142/148‑1 (MQ=255)
                                                                                                                     gCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCgg                           >  2:530903/1‑148 (MQ=255)
                                                                                                                        aCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTg                        >  1:2046390/1‑148 (MQ=255)
                                                                                                                        aCCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTg                        <  2:3244217/148‑1 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       >  1:2363644/1‑148 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       >  1:974385/1‑148 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       >  1:2927980/1‑148 (MQ=255)
                                                                                                                           ggCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTg                     >  1:2621798/1‑148 (MQ=255)
                                                                                                                           ggCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTg                     <  2:1748562/148‑1 (MQ=255)
                                                                                                                              cACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCtt                  <  2:2483596/148‑1 (MQ=255)
                                                                                                                              cACGGCGCGTTGTCGATAGAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCtt                  <  2:2485406/148‑1 (MQ=255)
                                                                                                                                  gcgcGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGc              >  1:2982755/1‑148 (MQ=255)
                                                                                                                                   cgcgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGcc             >  1:2232141/1‑148 (MQ=255)
                                                                                                                                    gcgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTCTCGCCa            >  1:1161840/1‑148 (MQ=255)
                                                                                                                                     cgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAg           >  2:2570822/1‑148 (MQ=255)
                                                                                                                                      gTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGt          <  1:1101570/148‑1 (MQ=255)
                                                                                                                                      gTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGt          <  2:2392779/148‑1 (MQ=255)
                                                                                                                                       ttGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGtt         <  1:1822119/148‑1 (MQ=255)
                                                                                                                                         gTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTg       <  1:2424064/148‑1 (MQ=255)
                                                                                                                                         gTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTg       <  2:1028045/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCAT  >  CP009273/667754‑668043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAG  >  CP009273/667751‑668046
                                                                                                                                                    |                                                                                                                                                   
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAG                                                                                                                                                    <  SRR19973066.964712/150‑1 (MQ=60)
  AGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCC                                                                                                                                                  >  SRR19973066.3372021/1‑150 (MQ=60)
  AGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCC                                                                                                                                                  >  SRR19973066.1761144/1‑150 (MQ=60)
  AGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCC                                                                                                                                                  >  SRR19973066.2610739/1‑150 (MQ=60)
   GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCT                                                                                                                                                 <  SRR19973066.3052919/150‑1 (MQ=60)
          CGCGAACCTGTTCGTCCGGTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTT                                                                                                                                          <  SRR19973066.1216871/150‑1 (MQ=60)
            CGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTC                                                                                                                                        >  SRR19973066.1875180/1‑150 (MQ=60)
            CGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTC                                                                                                                                        <  SRR19973066.616418/150‑1 (MQ=60)
             GAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCA                                                                                                                                       <  SRR19973066.2782705/150‑1 (MQ=60)
              AACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAG                                                                                                                                      >  SRR19973066.1749105/1‑150 (MQ=60)
               ACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGT                                                                                                                                     >  SRR19973066.2729437/1‑150 (MQ=60)
                 CTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTC                                                                                                                                   <  SRR19973066.3271040/150‑1 (MQ=60)
                  TGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCC                                                                                                                                  >  SRR19973066.492771/1‑150 (MQ=60)
                  TGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCC                                                                                                                                  >  SRR19973066.492704/1‑150 (MQ=60)
                  TGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCC                                                                                                                                  <  SRR19973066.2392615/150‑1 (MQ=60)
                  TGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCC                                                                                                                                  <  SRR19973066.2391982/150‑1 (MQ=60)
                      CTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCC                                                                                                                              >  SRR19973066.2718885/1‑150 (MQ=60)
                      CTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTAGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCC                                                                                                                              >  SRR19973066.2718798/1‑150 (MQ=60)
                          CGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAG                                                                                                                          <  SRR19973066.1378797/150‑1 (MQ=60)
                           GTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGC                                                                                                                         >  SRR19973066.2518819/1‑150 (MQ=60)
                           GTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGC                                                                                                                         >  SRR19973066.1494576/1‑150 (MQ=60)
                                 TCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAG                                                                                                                   <  SRR19973066.443299/150‑1 (MQ=60)
                                   CACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCA                                                                                                                 >  SRR19973066.1228897/1‑150 (MQ=60)
                                   CACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCA                                                                                                                 >  SRR19973066.3245635/1‑150 (MQ=60)
                                    ACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAG                                                                                                                <  SRR19973066.3442455/150‑1 (MQ=60)
                                    ACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAG                                                                                                                <  SRR19973066.1112713/150‑1 (MQ=60)
                                    ACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAG                                                                                                                <  SRR19973066.1111300/150‑1 (MQ=60)
                                    ACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAG                                                                                                                >  SRR19973066.843386/1‑150 (MQ=60)
                                    ACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAG                                                                                                                <  SRR19973066.1112775/150‑1 (MQ=60)
                                     CCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGA                                                                                                               <  SRR19973066.1525010/150‑1 (MQ=60)
                                      CGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAT                                                                                                              <  SRR19973066.1267061/150‑1 (MQ=60)
                                      CGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAT                                                                                                              <  SRR19973066.221450/150‑1 (MQ=60)
                                      CGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAT                                                                                                              <  SRR19973066.3188395/150‑1 (MQ=60)
                                       GGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATG                                                                                                             >  SRR19973066.2036554/1‑150 (MQ=60)
                                         AACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTG                                                                                                           >  SRR19973066.2131687/1‑150 (MQ=60)
                                             GTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGG                                                                                                       >  SRR19973066.1575578/1‑150 (MQ=60)
                                             GTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGG                                                                                                       <  SRR19973066.3329310/150‑1 (MQ=60)
                                              TGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGG                                                                                                      <  SRR19973066.879182/150‑1 (MQ=60)
                                              TGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGG                                                                                                      >  SRR19973066.3212107/1‑150 (MQ=60)
                                               GATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGT                                                                                                     >  SRR19973066.610536/1‑150 (MQ=60)
                                               GATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGT                                                                                                     >  SRR19973066.139981/1‑150 (MQ=60)
                                               GATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGT                                                                                                     >  SRR19973066.2410468/1‑150 (MQ=60)
                                                 TTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGA                                                                                                   <  SRR19973066.18099/150‑1 (MQ=60)
                                                 TTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGA                                                                                                   <  SRR19973066.2770964/150‑1 (MQ=60)
                                                   TTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAAC                                                                                                 <  SRR19973066.2999362/150‑1 (MQ=60)
                                                    TGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACG                                                                                                >  SRR19973066.2403341/1‑150 (MQ=60)
                                                         CGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTA                                                                                           <  SRR19973066.1373254/150‑1 (MQ=60)
                                                           GACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAG                                                                                         <  SRR19973066.1246597/150‑1 (MQ=60)
                                                             CTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCA                                                                                       >  SRR19973066.2526631/1‑150 (MQ=60)
                                                              TTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCAT                                                                                      <  SRR19973066.2592821/150‑1 (MQ=60)
                                                                 ACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACG                                                                                   >  SRR19973066.3095207/1‑150 (MQ=60)
                                                                  CCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGG                                                                                  >  SRR19973066.2239378/1‑150 (MQ=60)
                                                                    GTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAC                                                                                >  SRR19973066.2059780/1‑150 (MQ=60)
                                                                    GTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAC                                                                                <  SRR19973066.1904757/150‑1 (MQ=60)
                                                                    GTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAC                                                                                <  SRR19973066.2565230/150‑1 (MQ=60)
                                                                    GTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGAC                                                                                <  SRR19973066.2567602/150‑1 (MQ=60)
                                                                      TAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAA                                                                              >  SRR19973066.3346752/1‑150 (MQ=60)
                                                                      TAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGANATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAA                                                                              >  SRR19973066.1820370/1‑150 (MQ=60)
                                                                       ANCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCGTCGCCTTTCAGTTACTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAC                                                                             >  SRR19973066.1542038/1‑150 (MQ=60)
                                                                       AACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATTCGGACAAC                                                                             >  SRR19973066.1541828/1‑150 (MQ=60)
                                                                       AACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAC                                                                             <  SRR19973066.3292335/150‑1 (MQ=60)
                                                                        ACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCGTTCAGTTCCTGCCCCAGCGTGAAGCAGATGTGCGGGGGGAACGGGTTAAGCATACGGACAACG                                                                            >  SRR19973066.911480/1‑150 (MQ=60)
                                                                         CCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGG                                                                           >  SRR19973066.715101/1‑150 (MQ=60)
                                                                                  CGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTG                                                                  >  SRR19973066.2132066/1‑150 (MQ=60)
                                                                                     CCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTT                                                               <  SRR19973066.1591658/150‑1 (MQ=60)
                                                                                     CCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTT                                                               <  SRR19973066.1591656/150‑1 (MQ=60)
                                                                                     CCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTT                                                               <  SRR19973066.1413960/150‑1 (MQ=60)
                                                                                       AGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCC                                                             >  SRR19973066.1089168/1‑150 (MQ=60)
                                                                                        GCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCT                                                            <  SRR19973066.544062/150‑1 (MQ=60)
                                                                                         CGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTG                                                           <  SRR19973066.1649985/150‑1 (MQ=60)
                                                                                         CGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTG                                                           <  SRR19973066.1649994/150‑1 (MQ=60)
                                                                                           TGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCA                                                         >  SRR19973066.256652/1‑150 (MQ=60)
                                                                                           TGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCA                                                         >  SRR19973066.915928/1‑150 (MQ=60)
                                                                                           TGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCA                                                         >  SRR19973066.700140/1‑150 (MQ=60)
                                                                                              AGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCA                                                      >  SRR19973066.2795390/1‑150 (MQ=60)
                                                                                                   TCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCG                                                 <  SRR19973066.2679092/150‑1 (MQ=60)
                                                                                                   TCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCG                                                 <  SRR19973066.759527/150‑1 (MQ=60)
                                                                                                        CATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATC                                            <  SRR19973066.3212319/150‑1 (MQ=60)
                                                                                                               TTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCG                                     <  SRR19973066.3526385/150‑1 (MQ=60)
                                                                                                                   CGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCAT                                 <  SRR19973066.2075507/150‑1 (MQ=60)
                                                                                                                    GTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATC                                >  SRR19973066.583884/1‑150 (MQ=60)
                                                                                                                    GTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATC                                >  SRR19973066.584124/1‑150 (MQ=60)
                                                                                                                     TCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCG                               <  SRR19973066.1878014/150‑1 (MQ=60)
                                                                                                                     TCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCG                               <  SRR19973066.2982063/150‑1 (MQ=60)
                                                                                                                     TCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCG                               <  SRR19973066.2981295/150‑1 (MQ=60)
                                                                                                                       AGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGT                             <  SRR19973066.1916677/150‑1 (MQ=60)
                                                                                                                       AGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGT                             >  SRR19973066.1330985/1‑150 (MQ=60)
                                                                                                                        GCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGNGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTT                            <  SRR19973066.1818251/150‑1 (MQ=60)
                                                                                                                        GCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTT                            <  SRR19973066.2730652/150‑1 (MQ=60)
                                                                                                                        GCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTT                            <  SRR19973066.693200/150‑1 (MQ=60)
                                                                                                                        GCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTT                            <  SRR19973066.237669/150‑1 (MQ=60)
                                                                                                                        GCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTT                            <  SRR19973066.2728777/150‑1 (MQ=60)
                                                                                                                         CAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTG                           >  SRR19973066.2060623/1‑150 (MQ=60)
                                                                                                                          AACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGG                          >  SRR19973066.2379655/1‑150 (MQ=60)
                                                                                                                          AACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGG                          >  SRR19973066.981555/1‑150 (MQ=60)
                                                                                                                          AACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGG                          >  SRR19973066.2947479/1‑150 (MQ=60)
                                                                                                                          AACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGG                          >  SRR19973066.143310/1‑150 (MQ=60)
                                                                                                                            CCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTG                        >  SRR19973066.2639486/1‑150 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                   <  SRR19973066.3463269/150‑1 (MQ=60)
                                                                                                                                   CGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGC                 >  SRR19973066.3002460/1‑150 (MQ=60)
                                                                                                                                    GGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCC                >  SRR19973066.2247551/1‑150 (MQ=60)
                                                                                                                                     GCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTCTCGCCA               >  SRR19973066.1170082/1‑150 (MQ=60)
                                                                                                                                      CGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAG              <  SRR19973066.1107653/150‑1 (MQ=60)
                                                                                                                                        CGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTT            <  SRR19973066.2943710/150‑1 (MQ=60)
                                                                                                                                         GTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTT           <  SRR19973066.3111853/150‑1 (MQ=60)
                                                                                                                                         GTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTT           >  SRR19973066.95738/1‑150 (MQ=60)
                                                                                                                                         GTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTT           <  SRR19973066.1109566/150‑1 (MQ=60)
                                                                                                                                         GTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTT           >  SRR19973066.186319/1‑150 (MQ=60)
                                                                                                                                         GTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTT           >  SRR19973066.186879/1‑150 (MQ=60)
                                                                                                                                          TTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTG          <  SRR19973066.1835110/150‑1 (MQ=60)
                                                                                                                                            GTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTT        <  SRR19973066.2440523/150‑1 (MQ=60)
                                                                                                                                               GATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCAT     >  SRR19973066.743320/1‑150 (MQ=60)
                                                                                                                                                  ATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAG  <  SRR19973066.263068/150‑1 (MQ=60)
                                                                                                                                                  ATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAG  <  SRR19973066.263071/150‑1 (MQ=60)
                                                                                                                                                  ATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAG  <  SRR19973066.2833122/150‑1 (MQ=60)
                                                                                                                                                  ATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAG  <  SRR19973066.1038461/150‑1 (MQ=60)
                                                                                                                                                  ATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAG  <  SRR19973066.1038450/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATCAG  >  CP009273/667751‑668046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑