Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 667,899 C→A 100% D781Y (GAT→TAT)  leuS ← leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273667,8990CA97.4% 600.1 / 6.2 193D781Y (GAT→TAT) leuSleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (74/114);  total (77/116)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCAT  >  CP009273/667754‑668043
                                                                                                                                                 |                                                                                                                                                
gCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagc                                                                                                                                                <  1:915209/148‑1 (MQ=255)
 cACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcc                                                                                                                                               <  1:1132938/148‑1 (MQ=255)
   cGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcctt                                                                                                                                             >  2:1418813/1‑148 (MQ=255)
   cGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcctt                                                                                                                                             >  1:482760/1‑148 (MQ=255)
   cGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagcctt                                                                                                                                             <  2:566605/148‑1 (MQ=255)
    gTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATAtagccttc                                                                                                                                            <  2:2857425/148‑1 (MQ=255)
          gAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCttt                                                                                                                                      <  1:602803/148‑1 (MQ=255)
            aCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                    >  2:2740162/1‑148 (MQ=255)
            aCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                    >  2:2688048/1‑148 (MQ=255)
            aCCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCa                                                                                                                                    >  2:1699396/1‑148 (MQ=255)
             ccTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAg                                                                                                                                   <  2:2752662/148‑1 (MQ=255)
             ccTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAg                                                                                                                                   >  2:2841709/1‑148 (MQ=255)
              cTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGt                                                                                                                                  >  1:1940436/1‑148 (MQ=255)
               tGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGtt                                                                                                                                 >  1:1579520/1‑148 (MQ=255)
                     tcCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGcc                                                                                                                           <  2:2844159/148‑1 (MQ=255)
                       cGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCcac                                                                                                                         <  2:42771/148‑1 (MQ=255)
                       cGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCcac                                                                                                                         <  2:1822059/148‑1 (MQ=255)
                          tGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGc                                                                                                                      >  2:1239570/1‑148 (MQ=255)
                             gTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTg                                                                                                                   <  1:2981428/148‑1 (MQ=255)
                              tCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGa                                                                                                                  >  1:1698753/1‑148 (MQ=255)
                              tCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGa                                                                                                                  >  2:3035443/1‑148 (MQ=255)
                                cACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAg                                                                                                                <  2:526295/148‑1 (MQ=255)
                                cACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAg                                                                                                                <  2:2018499/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             <  1:2252543/148‑1 (MQ=255)
                                   cGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAg                                                                                                             >  1:1465954/1‑148 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          <  2:303415/148‑1 (MQ=255)
                                      aaCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtg                                                                                                          <  1:394001/148‑1 (MQ=255)
                                       aCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgt                                                                                                         <  2:2664491/148‑1 (MQ=255)
                                       aCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgt                                                                                                         >  2:3315878/1‑148 (MQ=255)
                                       aCGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgt                                                                                                         <  2:2433309/148‑1 (MQ=255)
                                        cGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgtg                                                                                                        <  2:1063722/148‑1 (MQ=255)
                                        cGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgtg                                                                                                        <  2:727668/148‑1 (MQ=255)
                                        cGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAtgtg                                                                                                        >  1:1323245/1‑148 (MQ=255)
                                         ggTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGc                                                                                                       <  2:2198708/148‑1 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    <  2:1452354/148‑1 (MQ=255)
                                            gATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCggg                                                                                                    >  2:224946/1‑148 (MQ=255)
                                             aTTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCgggg                                                                                                   >  2:627452/1‑148 (MQ=255)
                                                 tGGCACGGACTTGACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGaa                                                                                               <  2:2474853/148‑1 (MQ=255)
                                                  ggCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAAc                                                                                              <  1:1498946/148‑1 (MQ=255)
                                                  ggCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAAc                                                                                              <  2:2806517/148‑1 (MQ=255)
                                                   gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACg                                                                                             >  1:2137526/1‑148 (MQ=255)
                                                   gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACg                                                                                             >  1:1259444/1‑148 (MQ=255)
                                                   gCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACg                                                                                             >  1:3008008/1‑148 (MQ=255)
                                                    cACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACgg                                                                                            <  1:2448859/148‑1 (MQ=255)
                                                    cACGGACTTTACCGTGAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACgg                                                                                            <  2:2288412/148‑1 (MQ=255)
                                                        gACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTa                                                                                        <  1:253586/148‑1 (MQ=255)
                                                        gACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTa                                                                                        <  2:3280391/148‑1 (MQ=255)
                                                        gACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTa                                                                                        <  1:3248436/148‑1 (MQ=255)
                                                        gACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTa                                                                                        <  2:3280402/148‑1 (MQ=255)
                                                              aCCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATa                                                                                  <  2:330522/148‑1 (MQ=255)
                                                               ccGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAc                                                                                 >  1:1585491/1‑148 (MQ=255)
                                                               ccGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAc                                                                                 >  1:3389487/1‑148 (MQ=255)
                                                                  ttAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACCGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGa                                                                              >  1:1005354/1‑148 (MQ=255)
                                                                  ttAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGa                                                                              >  1:290461/1‑148 (MQ=255)
                                                                    aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACa                                                                            <  2:1543887/148‑1 (MQ=255)
                                                                    aaCCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACa                                                                            <  1:3415248/148‑1 (MQ=255)
                                                                            ccacGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcag                                                                    >  2:374525/1‑148 (MQ=255)
                                                                               cGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGTATACGGACAACGGCcagcag                                                                 <  1:3041163/148‑1 (MQ=255)
                                                                               cGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcagcag                                                                 >  2:3421877/1‑148 (MQ=255)
                                                                               cGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCcagcag                                                                 <  2:2114385/148‑1 (MQ=255)
                                                                                gACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGt                                                                <  1:1213741/148‑1 (MQ=255)
                                                                                 aCCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTg                                                               <  1:2692744/148‑1 (MQ=255)
                                                                                  ccAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGc                                                              <  2:937395/148‑1 (MQ=255)
                                                                                     gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                                           <  1:3323962/148‑1 (MQ=255)
                                                                                     gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                                           <  2:1931176/148‑1 (MQ=255)
                                                                                     gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                                           <  2:3348874/148‑1 (MQ=255)
                                                                                     gCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTc                                                           <  1:2872020/148‑1 (MQ=255)
                                                                                            gTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATc                                                    >  1:632050/1‑148 (MQ=255)
                                                                                             tCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCa                                                   <  2:1469452/148‑1 (MQ=255)
                                                                                              cTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCag                                                  <  2:1640652/148‑1 (MQ=255)
                                                                                               ttCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCaga                                                 <  2:496126/148‑1 (MQ=255)
                                                                                                tCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCagag                                                <  1:3489955/148‑1 (MQ=255)
                                                                                                tCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCagag                                                <  1:3202131/148‑1 (MQ=255)
                                                                                                  caccaTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAgcg                                              <  1:858859/148‑1 (MQ=255)
                                                                                                     caTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGa                                           >  2:301007/1‑148 (MQ=255)
                                                                                                      aTCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAt                                          <  2:2488645/148‑1 (MQ=255)
                                                                                                        cGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATcc                                        <  2:1906589/148‑1 (MQ=255)
                                                                                                           tttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGc                                     >  1:1826496/1‑148 (MQ=255)
                                                                                                            ttttCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCt                                    <  1:989204/148‑1 (MQ=255)
                                                                                                                cGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGcc                                <  2:1495722/148‑1 (MQ=255)
                                                                                                                  tCAGCAACCGGCCACTGCGCGTTGTAGATATAGCGTTCGCCTTTCATTTACTGCCACAGCGTTAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCAt                              <  1:749004/148‑1 (MQ=255)
                                                                                                                  tCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCAt                              <  1:1418813/148‑1 (MQ=255)
                                                                                                                   cAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATc                             >  2:2372159/1‑148 (MQ=255)
                                                                                                                   cAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATc                             <  2:825762/148‑1 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       <  2:857584/148‑1 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       <  1:1789977/148‑1 (MQ=255)
                                                                                                                         ccGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTgg                       <  2:2429020/148‑1 (MQ=255)
                                                                                                                            gCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGc                    <  1:1816481/148‑1 (MQ=255)
                                                                                                                            gCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGc                    <  1:789194/148‑1 (MQ=255)
                                                                                                                              cACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCtt                  <  1:1992831/148‑1 (MQ=255)
                                                                                                                                cGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGAGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTc                >  1:419722/1‑148 (MQ=255)
                                                                                                                                  gcgcGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGc              >  1:1911882/1‑148 (MQ=255)
                                                                                                                                  gcgcGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAAGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGc              >  1:3196328/1‑148 (MQ=255)
                                                                                                                                     cgTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAg           >  1:725529/1‑148 (MQ=255)
                                                                                                                                      gTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGt          <  1:248182/148‑1 (MQ=255)
                                                                                                                                      gTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGt          <  2:1971651/148‑1 (MQ=255)
                                                                                                                                       ttGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGtt         >  2:3201227/1‑148 (MQ=255)
                                                                                                                                         gTCGATATAGCCTTCGCCTGTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTg       <  2:2970459/148‑1 (MQ=255)
                                                                                                                                             atatAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtca   <  2:3456247/148‑1 (MQ=255)
                                                                                                                                              tataGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTtcat  <  2:90185/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCAT  >  CP009273/667754‑668043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATC  >  CP009273/667751‑668044
                                                                                                                                                    |                                                                                                                                                 
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAG                                                                                                                                                  <  SRR19973065.2497209/150‑1 (MQ=60)
  AGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCC                                                                                                                                                <  SRR19973065.121627/150‑1 (MQ=60)
  AGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCC                                                                                                                                                >  SRR19973065.3413594/1‑150 (MQ=60)
   GCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCT                                                                                                                                               <  SRR19973065.921183/150‑1 (MQ=60)
    CACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTT                                                                                                                                              <  SRR19973065.1140289/150‑1 (MQ=60)
    CACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTT                                                                                                                                              >  SRR19973065.1971070/1‑150 (MQ=60)
    CACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTT                                                                                                                                              >  SRR19973065.486043/1‑150 (MQ=60)
    CACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTT                                                                                                                                              >  SRR19973065.2504306/1‑150 (MQ=60)
    CACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTT                                                                                                                                              >  SRR19973065.485023/1‑150 (MQ=60)
         TCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCT                                                                                                                                         <  SRR19973065.1242858/150‑1 (MQ=60)
          CGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTT                                                                                                                                        <  SRR19973065.1277171/150‑1 (MQ=60)
            CGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTC                                                                                                                                      >  SRR19973065.3190778/1‑150 (MQ=60)
             GAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCA                                                                                                                                     <  SRR19973065.606600/150‑1 (MQ=60)
             GAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCA                                                                                                                                     <  SRR19973065.606592/150‑1 (MQ=60)
               ACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGT                                                                                                                                   >  SRR19973065.1953127/1‑150 (MQ=60)
                CCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTT                                                                                                                                  >  SRR19973065.1590045/1‑150 (MQ=60)
                CCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTT                                                                                                                                  >  SRR19973065.1590125/1‑150 (MQ=60)
                 CTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTC                                                                                                                                 <  SRR19973065.76753/150‑1 (MQ=60)
                    TTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTG                                                                                                                              <  SRR19973065.1207454/150‑1 (MQ=60)
                    TTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTG                                                                                                                              >  SRR19973065.1505341/1‑150 (MQ=60)
                               CGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGA                                                                                                                   >  SRR19973065.1710081/1‑150 (MQ=60)
                                GTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAA                                                                                                                  <  SRR19973065.2999673/150‑1 (MQ=60)
                                    ACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAG                                                                                                              >  SRR19973065.1475360/1‑150 (MQ=60)
                                      CGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAT                                                                                                            <  SRR19973065.2266816/150‑1 (MQ=60)
                                      CGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGAT                                                                                                            <  SRR19973065.2024944/150‑1 (MQ=60)
                                         AACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTG                                                                                                         <  SRR19973065.396887/150‑1 (MQ=60)
                                         AACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTG                                                                                                         >  SRR19973065.1331993/1‑150 (MQ=60)
                                         AACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTG                                                                                                         <  SRR19973065.396753/150‑1 (MQ=60)
                                           CGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCG                                                                                                       >  SRR19973065.1838330/1‑150 (MQ=60)
                                            GGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGG                                                                                                      >  SRR19973065.845322/1‑150 (MQ=60)
                                            GGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGG                                                                                                      >  SRR19973065.967342/1‑150 (MQ=60)
                                                ATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTG                                                                                                  >  SRR19973065.140655/1‑150 (MQ=60)
                                                ATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGACCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTG                                                                                                  >  SRR19973065.1674922/1‑150 (MQ=60)
                                                 TTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGA                                                                                                 <  SRR19973065.465038/150‑1 (MQ=60)
                                                    TGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACG                                                                                              >  SRR19973065.2151279/1‑150 (MQ=60)
                                                    TGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACG                                                                                              >  SRR19973065.3026428/1‑150 (MQ=60)
                                                    TGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACG                                                                                              <  SRR19973065.230862/150‑1 (MQ=60)
                                                    TGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACG                                                                                              >  SRR19973065.1267743/1‑150 (MQ=60)
                                                     GGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGG                                                                                             >  SRR19973065.1734086/1‑150 (MQ=60)
                                                     GGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGG                                                                                             <  SRR19973065.1508611/150‑1 (MQ=60)
                                                     GGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGG                                                                                             <  SRR19973065.1508607/150‑1 (MQ=60)
                                                       CACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGT                                                                                           <  SRR19973065.2464289/150‑1 (MQ=60)
                                                        ACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTT                                                                                          >  SRR19973065.727519/1‑150 (MQ=60)
                                                           GACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTTCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAG                                                                                       >  SRR19973065.3477310/1‑150 (MQ=60)
                                                           GACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAG                                                                                       <  SRR19973065.255359/150‑1 (MQ=60)
                                                           GACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAG                                                                                       <  SRR19973065.3268057/150‑1 (MQ=60)
                                                                TACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAC                                                                                  >  SRR19973065.3410197/1‑150 (MQ=60)
                                                                TACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAC                                                                                  >  SRR19973065.3410216/1‑150 (MQ=60)
                                                                TACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATAC                                                                                  >  SRR19973065.1596065/1‑150 (MQ=60)
                                                                   CGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACCGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGA                                                                               >  SRR19973065.1012030/1‑150 (MQ=60)
                                                                   CGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGA                                                                               >  SRR19973065.292352/1‑150 (MQ=60)
                                                                       AACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAC                                                                           <  SRR19973065.3436117/150‑1 (MQ=60)
                                                                        ACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACG                                                                          <  SRR19973065.1827087/150‑1 (MQ=60)
                                                                           TGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCC                                                                       >  SRR19973065.188999/1‑150 (MQ=60)
                                                                           TGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCC                                                                       <  SRR19973065.2961221/150‑1 (MQ=60)
                                                                                 ACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGT                                                                 <  SRR19973065.2311026/150‑1 (MQ=60)
                                                                                 ACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGT                                                                 <  SRR19973065.1661926/150‑1 (MQ=60)
                                                                                  CGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGTATACGGACAACGGCCAGCAGTG                                                                <  SRR19973065.192931/150‑1 (MQ=60)
                                                                                  CGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGTATACGGACAACGGCCAGCAGTG                                                                <  SRR19973065.3059781/150‑1 (MQ=60)
                                                                                   GACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGC                                                               <  SRR19973065.1221590/150‑1 (MQ=60)
                                                                                    ACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCT                                                              <  SRR19973065.2709462/150‑1 (MQ=60)
                                                                                        GCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCT                                                          <  SRR19973065.2889621/150‑1 (MQ=60)
                                                                                        GCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCT                                                          <  SRR19973065.3344246/150‑1 (MQ=60)
                                                                                             GAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATC                                                     >  SRR19973065.636165/1‑150 (MQ=60)
                                                                                             GAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATC                                                     <  SRR19973065.1074294/150‑1 (MQ=60)
                                                                                              AGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCA                                                    <  SRR19973065.2215443/150‑1 (MQ=60)
                                                                                                   TCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCG                                               <  SRR19973065.3221409/150‑1 (MQ=60)
                                                                                                   TCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCG                                               <  SRR19973065.3266153/150‑1 (MQ=60)
                                                                                                   TCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCG                                               <  SRR19973065.3511179/150‑1 (MQ=60)
                                                                                                     CACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCG                                             <  SRR19973065.874792/150‑1 (MQ=60)
                                                                                                     CACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCG                                             <  SRR19973065.864397/150‑1 (MQ=60)
                                                                                                       CCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGAT                                           >  SRR19973065.2488677/1‑150 (MQ=60)
                                                                                                          TCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCT                                        <  SRR19973065.316439/150‑1 (MQ=60)
                                                                                                           CGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTG                                       >  SRR19973065.301705/1‑150 (MQ=60)
                                                                                                           CGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTG                                       >  SRR19973065.2598897/1‑150 (MQ=60)
                                                                                                            GCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGC                                      >  SRR19973065.574843/1‑150 (MQ=60)
                                                                                                            GCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGC                                      >  SRR19973065.1838531/1‑150 (MQ=60)
                                                                                                               TTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCG                                   <  SRR19973065.995773/150‑1 (MQ=60)
                                                                                                                     TCAGCAACCGGCCACTGCGCGTTGTAGATATAGCGTTCGCCTTTCATTTACTGCCACAGCGTTAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCG                             <  SRR19973065.754010/150‑1 (MQ=60)
                                                                                                                     TCAGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCG                             <  SRR19973065.1428073/150‑1 (MQ=60)
                                                                                                                       AGCAACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGT                           >  SRR19973065.997330/1‑150 (MQ=60)
                                                                                                                           ACCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGT                       <  SRR19973065.737206/150‑1 (MQ=60)
                                                                                                                            CCGGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTG                      <  SRR19973065.1801708/150‑1 (MQ=60)
                                                                                                                              GGCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCT                    <  SRR19973065.2906398/150‑1 (MQ=60)
                                                                                                                               GCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTT                   <  SRR19973065.794424/150‑1 (MQ=60)
                                                                                                                               GCCACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTT                   <  SRR19973065.1828445/150‑1 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                 <  SRR19973065.2005751/150‑1 (MQ=60)
                                                                                                                                 CACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGAGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTC                 >  SRR19973065.422651/1‑150 (MQ=60)
                                                                                                                                  ACGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCG                <  SRR19973065.3289208/150‑1 (MQ=60)
                                                                                                                                   CGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGC               >  SRR19973065.1924434/1‑150 (MQ=60)
                                                                                                                                   CGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGC               >  SRR19973065.3215634/1‑150 (MQ=60)
                                                                                                                                   CGGCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAAAGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGC               >  SRR19973065.3215588/1‑150 (MQ=60)
                                                                                                                                     GCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCA             <  SRR19973065.3037902/150‑1 (MQ=60)
                                                                                                                                     GCGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCA             <  SRR19973065.353737/150‑1 (MQ=60)
                                                                                                                                      CGCGTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAG            >  SRR19973065.730390/1‑150 (MQ=60)
                                                                                                                                         GTTGTCGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTT         <  SRR19973065.249930/150‑1 (MQ=60)
                                                                                                                                              CGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCA    <  SRR19973065.2818676/150‑1 (MQ=60)
                                                                                                                                              CGATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCA    <  SRR19973065.147222/150‑1 (MQ=60)
                                                                                                                                                ATATAGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATC  >  SRR19973065.1954527/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                 
CCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAACCTGCACCACGACCAGCGTGGAGTCTTCCACCATCGCTTTTTCGTCAGCAACCGGCCACGGCGCGTTGTCGATATCGCCTTCGCCTTTCAGTTCCTGCCACAGCGTGAAGCAGATGTGCGGGGTGAACGGGTTAAGCATACGGACAACGGCCAGCAGTGCTTCCTGCATCAGAGCGCGATCCTGCTCGCCATCGGTTGGTGCTTTCGCCAGTTTGTTCATC  >  CP009273/667751‑668044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑