Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,003,597 G→T D100Y (GAC→TAC)  rlmL → fused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,003,5970GT99.6% 750.6 / NA 225D100Y (GAC→TAC) rlmLfused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (112/112);  minor base A (1/0);  total (113/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCC  >  CP009273/1003453‑1003743
                                                                                                                                                |                                                                                                                                                  
cgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAcc                                                                                                                                               >  2:1100313/1‑150 (MQ=255)
cgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAcc                                                                                                                                               <  1:3634883/150‑1 (MQ=255)
cgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAcc                                                                                                                                               >  1:172972/1‑150 (MQ=255)
 gccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCa                                                                                                                                              >  2:3339224/1‑150 (MQ=255)
 gccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCa                                                                                                                                              >  1:975128/1‑150 (MQ=255)
  ccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCAt                                                                                                                                             <  1:1633470/150‑1 (MQ=255)
   cTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATc                                                                                                                                            <  2:2385716/150‑1 (MQ=255)
   cTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATc                                                                                                                                            <  1:3730827/150‑1 (MQ=255)
     ggCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCg                                                                                                                                          >  1:2257920/1‑150 (MQ=255)
         tCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAc                                                                                                                                      >  1:1054626/1‑150 (MQ=255)
            cgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagt                                                                                                                                   >  2:1772379/1‑150 (MQ=255)
             gTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtc                                                                                                                                  <  1:3550957/150‑1 (MQ=255)
               attatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtcag                                                                                                                                <  2:3726479/150‑1 (MQ=255)
               attatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagccag                                                                                                                                >  2:847716/1‑150 (MQ=255)
                 tatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTa                                                                                                                              >  2:3138831/1‑150 (MQ=255)
                     ttGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGgc                                                                                                                          >  1:471215/1‑150 (MQ=255)
                     ttGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGgc                                                                                                                          >  2:839669/1‑150 (MQ=255)
                     ttGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGgc                                                                                                                          >  1:1024671/1‑150 (MQ=255)
                      tGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGgcg                                                                                                                         >  1:906732/1‑150 (MQ=255)
                          gCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAt                                                                                                                     >  1:2557032/1‑150 (MQ=255)
                           cTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtg                                                                                                                    >  2:1375097/1‑150 (MQ=255)
                            tGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtga                                                                                                                   >  2:2870889/1‑150 (MQ=255)
                             gggCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaa                                                                                                                  >  1:538650/1‑150 (MQ=255)
                                cGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagt                                                                                                               <  2:2883589/150‑1 (MQ=255)
                                cGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagt                                                                                                               >  1:3027826/1‑150 (MQ=255)
                                cGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagt                                                                                                               <  2:250175/150‑1 (MQ=255)
                                 gAGTGTAAGGTGTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGTTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtg                                                                                                              <  1:729892/150‑1 (MQ=255)
                                  aGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtga                                                                                                             <  2:22053/150‑1 (MQ=255)
                                   gtgtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaa                                                                                                            >  2:620836/1‑150 (MQ=255)
                                    tgtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaaa                                                                                                           <  2:2776414/150‑1 (MQ=255)
                                     gtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaaag                                                                                                          <  2:1684708/150‑1 (MQ=255)
                                      tAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGa                                                                                                         >  1:2921111/1‑150 (MQ=255)
                                        aGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAcg                                                                                                       >  1:2001444/1‑150 (MQ=255)
                                              aCAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcg                                                                                                 >  2:1779820/1‑150 (MQ=255)
                                                   gATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGAt                                                                                            >  2:3031797/1‑150 (MQ=255)
                                                    aTTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATg                                                                                           <  2:698152/150‑1 (MQ=255)
                                                    aTTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATg                                                                                           <  2:1234406/150‑1 (MQ=255)
                                                       tAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCtt                                                                                        >  2:1431556/1‑150 (MQ=255)
                                                        aGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCttt                                                                                       >  1:3048548/1‑150 (MQ=255)
                                                        aGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCttt                                                                                       >  2:2122274/1‑150 (MQ=255)
                                                          aCCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCa                                                                                     <  1:1585738/150‑1 (MQ=255)
                                                            ctctATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAcg                                                                                   <  1:629631/150‑1 (MQ=255)
                                                              ctATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAcgcg                                                                                 >  2:2120747/1‑150 (MQ=255)
                                                               tATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCgg                                                                                <  1:1100313/150‑1 (MQ=255)
                                                                  ctcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCACCAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaaa                                                                             >  1:1360842/1‑150 (MQ=255)
                                                                   tcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaaaa                                                                            >  2:1317550/1‑150 (MQ=255)
                                                                    cGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaaaaa                                                                           <  1:2817913/150‑1 (MQ=255)
                                                                      gtgtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATc                                                                         >  1:252516/1‑150 (MQ=255)
                                                                        gtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTg                                                                       <  1:1254607/150‑1 (MQ=255)
                                                                         ttCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGc                                                                      >  2:785094/1‑150 (MQ=255)
                                                                           cAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcg                                                                    <  2:1054626/150‑1 (MQ=255)
                                                                            aGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcgc                                                                   >  2:38125/1‑150 (MQ=255)
                                                                                gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               >  2:289174/1‑150 (MQ=255)
                                                                                gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               >  1:3650959/1‑150 (MQ=255)
                                                                                gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               >  2:1741876/1‑150 (MQ=255)
                                                                                gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               <  2:2220639/150‑1 (MQ=255)
                                                                                 aTCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGTATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCa                                                              <  2:1576228/150‑1 (MQ=255)
                                                                                 aTCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCa                                                              <  1:931517/150‑1 (MQ=255)
                                                                                 aTCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCa                                                              >  2:1209132/1‑150 (MQ=255)
                                                                                 aTCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCa                                                              <  2:3577001/150‑1 (MQ=255)
                                                                                     aCTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATg                                                          >  1:1470212/1‑150 (MQ=255)
                                                                                     aCTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATg                                                          <  2:1873458/150‑1 (MQ=255)
                                                                                      cTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAACTGt                                                         >  2:3549372/1‑150 (MQ=255)
                                                                                      cTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGt                                                         <  1:81686/150‑1 (MQ=255)
                                                                                         gACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAGGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGa                                                      >  1:3240816/1‑150 (MQ=255)
                                                                                          aCAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAt                                                     <  2:3094087/150‑1 (MQ=255)
                                                                                             gagaTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgc                                                  <  1:3039297/150‑1 (MQ=255)
                                                                                              agaTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgcg                                                 <  1:1861809/150‑1 (MQ=255)
                                                                                              agaTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgcg                                                 >  1:2684097/1‑150 (MQ=255)
                                                                                              agaTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgcg                                                 >  1:2234658/1‑150 (MQ=255)
                                                                                                aTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGAt                                               >  2:1902868/1‑150 (MQ=255)
                                                                                                 tGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATg                                              >  1:1738341/1‑150 (MQ=255)
                                                                                                   tttAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATgcg                                            <  2:3650416/150‑1 (MQ=255)
                                                                                                      aaTCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCg                                         <  2:1249602/150‑1 (MQ=255)
                                                                                                       aTCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCgg                                        <  2:3578283/150‑1 (MQ=255)
                                                                                                       aTCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCgg                                        >  2:3133262/1‑150 (MQ=255)
                                                                                                          cTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGata                                     <  1:3026280/150‑1 (MQ=255)
                                                                                                           tGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGatat                                    <  2:2090226/150‑1 (MQ=255)
                                                                                                             gcgcGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATcc                                  <  1:211964/150‑1 (MQ=255)
                                                                                                              cgcgACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCcg                                 >  2:2434030/1‑150 (MQ=255)
                                                                                                                cgACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCcgcg                               >  1:2750404/1‑150 (MQ=255)
                                                                                                                 gACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGt                              <  1:1089772/150‑1 (MQ=255)
                                                                                                                 gACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGt                              >  1:1420426/1‑150 (MQ=255)
                                                                                                                       cGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGt                        >  1:2171415/1‑150 (MQ=255)
                                                                                                                       cGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGt                        >  1:3577411/1‑150 (MQ=255)
                                                                                                                        gCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTc                       <  1:596049/150‑1 (MQ=255)
                                                                                                                           gTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTgg                    >  1:787373/1‑150 (MQ=255)
                                                                                                                            tCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGc                   <  1:3339224/150‑1 (MQ=255)
                                                                                                                             ccACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCt                  >  1:3401168/1‑150 (MQ=255)
                                                                                                                              cACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTg                 >  2:2159737/1‑150 (MQ=255)
                                                                                                                                 ttCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCAt              <  2:227780/150‑1 (MQ=255)
                                                                                                                                   cAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATaa            <  2:3763197/150‑1 (MQ=255)
                                                                                                                                    aGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATaaa           >  1:1243730/1‑150 (MQ=255)
                                                                                                                                     gTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAg          <  1:1738418/150‑1 (MQ=255)
                                                                                                                                      tGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGa         <  2:538650/150‑1 (MQ=255)
                                                                                                                                      tGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTACACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGa         >  1:3425630/1‑150 (MQ=255)
                                                                                                                                       ggTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaa        >  1:1492467/1‑150 (MQ=255)
                                                                                                                                       ggTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaa        <  1:2928078/150‑1 (MQ=255)
                                                                                                                                        gTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaaa       <  2:476239/150‑1 (MQ=255)
                                                                                                                                        gTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaaa       <  2:921987/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCC  >  CP009273/1003453‑1003743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA  >  CP009273/1003448‑1003744
                                                                                                                                                     |                                                                                                                                                   
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATT                                                                                                                                                     >  SRR19973061.355704/1‑150 (MQ=60)
GGAGCCGCCTGGACTCGCGTATTATGTTGCCGCTGGGAGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATT                                                                                                                                                     <  SRR19973061.1112402/150‑1 (MQ=60)
     CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACC                                                                                                                                                >  SRR19973061.173503/1‑150 (MQ=60)
     CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACC                                                                                                                                                <  SRR19973061.3644175/150‑1 (MQ=60)
      GCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCA                                                                                                                                               >  SRR19973061.977610/1‑150 (MQ=60)
       CCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCAT                                                                                                                                              <  SRR19973061.1637098/150‑1 (MQ=60)
       CCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCAT                                                                                                                                              <  SRR19973061.1637559/150‑1 (MQ=60)
       CCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCAT                                                                                                                                              <  SRR19973061.1637594/150‑1 (MQ=60)
        CTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATC                                                                                                                                             <  SRR19973061.1582523/150‑1 (MQ=60)
        CTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATC                                                                                                                                             <  SRR19973061.3740371/150‑1 (MQ=60)
         TGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCC                                                                                                                                            >  SRR19973061.1096418/1‑150 (MQ=60)
          GGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCG                                                                                                                                           >  SRR19973061.2263607/1‑150 (MQ=60)
            CCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATAACACCATCCGCA                                                                                                                                         >  SRR19973061.3771455/1‑150 (MQ=60)
              TCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAC                                                                                                                                       >  SRR19973061.1057336/1‑150 (MQ=60)
               CGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACA                                                                                                                                      <  SRR19973061.486473/150‑1 (MQ=60)
                 CGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGT                                                                                                                                    >  SRR19973061.1738384/1‑150 (MQ=60)
                 CGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGT                                                                                                                                    >  SRR19973061.3557570/1‑150 (MQ=60)
                  GTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTC                                                                                                                                   <  SRR19973061.3560048/150‑1 (MQ=60)
                  GTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTC                                                                                                                                   <  SRR19973061.1660439/150‑1 (MQ=60)
                        TGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACG                                                                                                                             <  SRR19973061.641770/150‑1 (MQ=60)
                          TTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGC                                                                                                                           >  SRR19973061.472488/1‑150 (MQ=60)
                          TTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGC                                                                                                                           >  SRR19973061.1027290/1‑150 (MQ=60)
                           TGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCG                                                                                                                          >  SRR19973061.909061/1‑150 (MQ=60)
                               GCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAT                                                                                                                      >  SRR19973061.2563556/1‑150 (MQ=60)
                               GCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAT                                                                                                                      <  SRR19973061.2158704/150‑1 (MQ=60)
                                  TGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAA                                                                                                                   >  SRR19973061.3298655/1‑150 (MQ=60)
                                  GGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAA                                                                                                                   <  SRR19973061.3146909/150‑1 (MQ=60)
                                  GGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAA                                                                                                                   >  SRR19973061.540070/1‑150 (MQ=60)
                                     CGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGT                                                                                                                >  SRR19973061.3035630/1‑150 (MQ=60)
                                     CGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGT                                                                                                                >  SRR19973061.3035652/1‑150 (MQ=60)
                                     CGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGT                                                                                                                >  SRR19973061.3025756/1‑150 (MQ=60)
                                      GAGTGTATGGTGTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGTTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTG                                                                                                               <  SRR19973061.731804/150‑1 (MQ=60)
                                        GTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAA                                                                                                             <  SRR19973061.2424604/150‑1 (MQ=60)
                                          GTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAG                                                                                                           <  SRR19973061.3578541/150‑1 (MQ=60)
                                           TAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGA                                                                                                          >  SRR19973061.2928637/1‑150 (MQ=60)
                                           TAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGA                                                                                                          >  SRR19973061.2928614/1‑150 (MQ=60)
                                            AAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAC                                                                                                         >  SRR19973061.3659741/1‑150 (MQ=60)
                                             AGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACG                                                                                                        >  SRR19973061.2006457/1‑150 (MQ=60)
                                             AGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACG                                                                                                        <  SRR19973061.3714680/150‑1 (MQ=60)
                                               GTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCG                                                                                                      <  SRR19973061.270519/150‑1 (MQ=60)
                                                  TACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATC                                                                                                   >  SRR19973061.3354093/1‑150 (MQ=60)
                                                  TACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATC                                                                                                   >  SRR19973061.739224/1‑150 (MQ=60)
                                                       CGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGA                                                                                              >  SRR19973061.1396692/1‑150 (MQ=60)
                                                           TTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCT                                                                                          >  SRR19973061.3472648/1‑150 (MQ=60)
                                                             AGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTT                                                                                        >  SRR19973061.1150915/1‑150 (MQ=60)
                                                             AGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTT                                                                                        >  SRR19973061.3056430/1‑150 (MQ=60)
                                                               ACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCA                                                                                      <  SRR19973061.1589674/150‑1 (MQ=60)
                                                                 CTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACG                                                                                    <  SRR19973061.1127896/150‑1 (MQ=60)
                                                                 CTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACG                                                                                    <  SRR19973061.631207/150‑1 (MQ=60)
                                                                 CTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACG                                                                                    <  SRR19973061.631236/150‑1 (MQ=60)
                                                                    TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                 <  SRR19973061.1103129/150‑1 (MQ=60)
                                                                      TCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAA                                                                               <  SRR19973061.1483115/150‑1 (MQ=60)
                                                                       CTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCACCAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAA                                                                              >  SRR19973061.1364274/1‑150 (MQ=60)
                                                                         CGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAA                                                                            <  SRR19973061.2825142/150‑1 (MQ=60)
                                                                          GGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAAT                                                                           >  SRR19973061.3665857/1‑150 (MQ=60)
                                                                           GTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATC                                                                          >  SRR19973061.253250/1‑150 (MQ=60)
                                                                           GTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATC                                                                          >  SRR19973061.253220/1‑150 (MQ=60)
                                                                             GTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTG                                                                        <  SRR19973061.1257714/150‑1 (MQ=60)
                                                                                 AGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGC                                                                    >  SRR19973061.3051476/1‑150 (MQ=60)
                                                                                     GATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCC                                                                >  SRR19973061.3660285/1‑150 (MQ=60)
                                                                                      ATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCA                                                               <  SRR19973061.933893/150‑1 (MQ=60)
                                                                                      ATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCA                                                               <  SRR19973061.933039/150‑1 (MQ=60)
                                                                                          ACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATG                                                           >  SRR19973061.1473905/1‑150 (MQ=60)
                                                                                           CTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGT                                                          <  SRR19973061.81976/150‑1 (MQ=60)
                                                                                             GGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTG                                                        >  SRR19973061.1032895/1‑150 (MQ=60)
                                                                                             GGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTG                                                        <  SRR19973061.622421/150‑1 (MQ=60)
                                                                                              GACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAGGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGA                                                       >  SRR19973061.3249154/1‑150 (MQ=60)
                                                                                               ACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAT                                                      <  SRR19973061.2063184/150‑1 (MQ=60)
                                                                                                 AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCG                                                    >  SRR19973061.1426747/1‑150 (MQ=60)
                                                                                                  GAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGC                                                   <  SRR19973061.3047156/150‑1 (MQ=60)
                                                                                                   AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                  >  SRR19973061.2240167/1‑150 (MQ=60)
                                                                                                   AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                  >  SRR19973061.2240267/1‑150 (MQ=60)
                                                                                                   AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                  <  SRR19973061.1866546/150‑1 (MQ=60)
                                                                                                   AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                  <  SRR19973061.1866455/150‑1 (MQ=60)
                                                                                                   AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                  >  SRR19973061.2691005/1‑150 (MQ=60)
                                                                                                      TGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATG                                               >  SRR19973061.1742712/1‑150 (MQ=60)
                                                                                                         TTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGC                                            >  SRR19973061.2260828/1‑150 (MQ=60)
                                                                                                          TAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCC                                           >  SRR19973061.1312142/1‑150 (MQ=60)
                                                                                                              CCTGCCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                       >  SRR19973061.2889384/1‑150 (MQ=60)
                                                                                                               CTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATA                                      <  SRR19973061.3034099/150‑1 (MQ=60)
                                                                                                                TGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATAT                                     >  SRR19973061.57289/1‑150 (MQ=60)
                                                                                                                TGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATAT                                     >  SRR19973061.2659922/1‑150 (MQ=60)
                                                                                                                TGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATAT                                     <  SRR19973061.1895335/150‑1 (MQ=60)
                                                                                                                TGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATAT                                     <  SRR19973061.955363/150‑1 (MQ=60)
                                                                                                                  GCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGTTCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCC                                   <  SRR19973061.1435160/150‑1 (MQ=60)
                                                                                                                  GCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCC                                   <  SRR19973061.212596/150‑1 (MQ=60)
                                                                                                                     CGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCG                                >  SRR19973061.2757259/1‑150 (MQ=60)
                                                                                                                     CGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCG                                >  SRR19973061.2757486/1‑150 (MQ=60)
                                                                                                                      GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                               >  SRR19973061.1424007/1‑150 (MQ=60)
                                                                                                                      GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                               <  SRR19973061.1092565/150‑1 (MQ=60)
                                                                                                                      GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                               <  SRR19973061.1092247/150‑1 (MQ=60)
                                                                                                                         CTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAA                            >  SRR19973061.2250169/1‑150 (MQ=60)
                                                                                                                           TCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACG                          >  SRR19973061.3175869/1‑150 (MQ=60)
                                                                                                                            CGCTGTCCACTTCTGTGGTTTGAATTACACCTTCCGCTTCAGTCAGTACGGCGCGTTGATAGTGAAAGTCGAGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGT                         <  SRR19973061.3413413/150‑1 (MQ=60)
                                                                                                                            CGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGT                         >  SRR19973061.2176872/1‑150 (MQ=60)
                                                                                                                            CGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGT                         >  SRR19973061.3586583/1‑150 (MQ=60)
                                                                                                                             GCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTC                        <  SRR19973061.597601/150‑1 (MQ=60)
                                                                                                                              CTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCT                       >  SRR19973061.2993248/1‑150 (MQ=60)
                                                                                                                               TGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTG                      >  SRR19973061.799843/1‑150 (MQ=60)
                                                                                                                                GTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGG                     >  SRR19973061.789444/1‑150 (MQ=60)
                                                                                                                                 TCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGC                    <  SRR19973061.3347821/150‑1 (MQ=60)
                                                                                                                                  CCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCT                   <  SRR19973061.1257237/150‑1 (MQ=60)
                                                                                                                                  CCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCT                   >  SRR19973061.3409903/1‑150 (MQ=60)
                                                                                                                                   CACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTG                  >  SRR19973061.2809448/1‑150 (MQ=60)
                                                                                                                                     CTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCA                <  SRR19973061.849972/150‑1 (MQ=60)
                                                                                                                                     CTTCAGTGGTTTGAATTACACCATCCGCAACAGCCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCA                <  SRR19973061.849929/150‑1 (MQ=60)
                                                                                                                                       TCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATA              <  SRR19973061.3039632/150‑1 (MQ=60)
                                                                                                                                        CAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAA             <  SRR19973061.3616123/150‑1 (MQ=60)
                                                                                                                                         AGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAA            >  SRR19973061.1246814/1‑150 (MQ=60)
                                                                                                                                          GTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAG           <  SRR19973061.1742748/150‑1 (MQ=60)
                                                                                                                                          GTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAG           <  SRR19973061.1742789/150‑1 (MQ=60)
                                                                                                                                           TGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTACACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGA          >  SRR19973061.3434417/1‑150 (MQ=60)
                                                                                                                                            GGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAA         >  SRR19973061.1496211/1‑150 (MQ=60)
                                                                                                                                            GGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAA         <  SRR19973061.2935607/150‑1 (MQ=60)
                                                                                                                                              TTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAC       <  SRR19973061.1836030/150‑1 (MQ=60)
                                                                                                                                                   ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA  <  SRR19973061.422950/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA  >  CP009273/1003448‑1003744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑