Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,003,597 G→T 100% D100Y (GAC→TAC)  rlmL → fused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,003,5970GT99.2% 849.9 / ‑2.8 258D100Y (GAC→TAC) rlmLfused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (131/125);  total (133/125)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 258 total aligned reads displayed.

GCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCC  >  CP009273/1003451‑1003743
                                                                                                                                                  |                                                                                                                                                  
  cgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAcc                                                                                                                                               <  1:29353/150‑1 (MQ=255)
  cgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAcc                                                                                                                                               <  1:2300218/150‑1 (MQ=255)
     cTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATc                                                                                                                                            >  1:2609059/1‑150 (MQ=255)
      tGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATcc                                                                                                                                           <  1:3723677/150‑1 (MQ=255)
      tGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATcc                                                                                                                                           >  2:584285/1‑150 (MQ=255)
         ccTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCa                                                                                                                                        >  2:2104209/1‑150 (MQ=255)
            cgcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGTGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGAAAca                                                                                                                                     >  2:5145043/1‑150 (MQ=255)
            cgcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAca                                                                                                                                     <  2:1528254/150‑1 (MQ=255)
            cgcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAca                                                                                                                                     <  2:78451/150‑1 (MQ=255)
             gcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcag                                                                                                                                    <  1:4925876/150‑1 (MQ=255)
             gcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcag                                                                                                                                    <  2:3630878/150‑1 (MQ=255)
             gcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcag                                                                                                                                    >  2:1188138/1‑150 (MQ=255)
                tattatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtca                                                                                                                                 <  2:271827/150‑1 (MQ=255)
                tattatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtca                                                                                                                                 <  1:4854937/150‑1 (MQ=255)
                tattatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtca                                                                                                                                 <  2:4346622/150‑1 (MQ=255)
                 attatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtcag                                                                                                                                >  2:568291/1‑150 (MQ=255)
                 attatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtcag                                                                                                                                >  1:1571089/1‑150 (MQ=255)
                  ttatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtcagt                                                                                                                               <  1:2383742/150‑1 (MQ=255)
                       ttGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGgc                                                                                                                          >  1:5036181/1‑150 (MQ=255)
                          ccGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGcgcg                                                                                                                       <  2:1971280/150‑1 (MQ=255)
                          ccGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGcgcg                                                                                                                       <  1:104264/150‑1 (MQ=255)
                           agCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGa                                                                                                                      >  1:2057707/2‑150 (MQ=255)
                             cTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtg                                                                                                                    <  1:2976952/150‑1 (MQ=255)
                              tGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgc                                                                                                                   >  2:3430893/1‑149 (MQ=255)
                              tGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtga                                                                                                                   >  2:4319091/1‑150 (MQ=255)
                              tGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtga                                                                                                                   <  2:3875598/150‑1 (MQ=255)
                                  cGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagt                                                                                                               <  2:407001/150‑1 (MQ=255)
                                  cGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagt                                                                                                               <  2:406849/150‑1 (MQ=255)
                                   gAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtg                                                                                                              >  2:305604/1‑150 (MQ=255)
                                      tgtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaaa                                                                                                           <  2:1694747/150‑1 (MQ=255)
                                      tgtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaaa                                                                                                           <  2:1556352/150‑1 (MQ=255)
                                        tAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGa                                                                                                         >  2:1268531/1‑150 (MQ=255)
                                        tAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGa                                                                                                         <  2:2394206/150‑1 (MQ=255)
                                         aaGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAc                                                                                                        >  2:3329962/1‑150 (MQ=255)
                                          aGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAcg                                                                                                       <  1:1206018/150‑1 (MQ=255)
                                               tACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtc                                                                                                  <  2:284650/150‑1 (MQ=255)
                                               tACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtc                                                                                                  >  1:4310146/1‑150 (MQ=255)
                                               tACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtc                                                                                                  <  2:1372360/150‑1 (MQ=255)
                                                aCAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcg                                                                                                 >  2:2414381/1‑150 (MQ=255)
                                                    cGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGa                                                                                             <  1:3134552/150‑1 (MQ=255)
                                                       tttAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGAGGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGACAGACGCGATCGTCGATGc                                                                                          >  2:2236367/1‑150 (MQ=255)
                                                          aGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCttt                                                                                       <  2:3157771/150‑1 (MQ=255)
                                                            aCCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCa                                                                                     <  1:77948/150‑1 (MQ=255)
                                                              ctctATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAcg                                                                                   <  1:1990309/150‑1 (MQ=255)
                                                                 tATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCgg                                                                                <  1:3228902/150‑1 (MQ=255)
                                                                 tATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCgg                                                                                <  1:1924808/150‑1 (MQ=255)
                                                                   tctcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaa                                                                              <  1:3057869/150‑1 (MQ=255)
                                                                    ctcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaaa                                                                             >  1:2088758/1‑150 (MQ=255)
                                                                    ctcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaaa                                                                             <  1:4810072/150‑1 (MQ=255)
                                                                    ctcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaaa                                                                             <  2:3569608/150‑1 (MQ=255)
                                                                        gtgtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATc                                                                         <  1:542299/150‑1 (MQ=255)
                                                                             cAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcg                                                                    >  1:2734635/1‑150 (MQ=255)
                                                                              aGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcgc                                                                   >  2:1853630/1‑150 (MQ=255)
                                                                              aGGCGAACAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGAGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAAACTGAcgc                                                                   >  2:2695213/1‑150 (MQ=255)
                                                                               ggCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcgcg                                                                  <  2:4841317/150‑1 (MQ=255)
                                                                                  gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               >  1:1182510/1‑150 (MQ=255)
                                                                                     cAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCaaa                                                            >  1:3568048/1‑150 (MQ=255)
                                                                                     cAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCaaa                                                            >  1:3393320/1‑150 (MQ=255)
                                                                                     cAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCaaa                                                            >  2:2348544/1‑150 (MQ=255)
                                                                                        cTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGt                                                         >  1:3929208/1‑150 (MQ=255)
                                                                                            aCAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAt                                                     >  1:4482164/1‑150 (MQ=255)
                                                                                            aCAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAt                                                     <  1:4919190/150‑1 (MQ=255)
                                                                                             cAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATc                                                    >  1:3779399/1‑150 (MQ=255)
                                                                                             cAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATc                                                    <  1:937532/150‑1 (MQ=255)
                                                                                              agagATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAATTCTGCCGCGTCCAAATGTTGATcg                                                   <  1:174646/150‑1 (MQ=255)
                                                                                               gagaTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgc                                                  <  1:4129213/150‑1 (MQ=255)
                                                                                                   tGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATg                                              <  1:4589579/150‑1 (MQ=255)
                                                                                                     tttAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATgcg                                            <  2:2922716/150‑1 (MQ=255)
                                                                                                         aTCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCgg                                        <  2:1990968/150‑1 (MQ=255)
                                                                                                         aTCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCgg                                        <  1:2486419/150‑1 (MQ=255)
                                                                                                          tCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAACGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGa                                       <  2:928865/150‑1 (MQ=255)
                                                                                                           ccTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGat                                      >  1:4386195/1‑150 (MQ=255)
                                                                                                           ccTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATAGCGATGCGCCGGat                                      >  2:1071644/1‑150 (MQ=255)
                                                                                                            cTGGCGCGTCCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGata                                     >  1:1638378/1‑150 (MQ=255)
                                                                                                            cTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGata                                     >  2:5106566/1‑150 (MQ=255)
                                                                                                             tGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGatat                                    >  2:4934218/1‑150 (MQ=255)
                                                                                                              ggCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATc                                   >  2:1227825/1‑150 (MQ=255)
                                                                                                                   gACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGt                              >  2:4723232/1‑150 (MQ=255)
                                                                                                                      cTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTaa                           >  2:3347515/1‑150 (MQ=255)
                                                                                                                      cTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTaa                           >  2:2573903/1‑150 (MQ=255)
                                                                                                                       ttCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAAc                          <  2:4666613/150‑1 (MQ=255)
                                                                                                                       ttCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAAc                          >  2:1946123/1‑150 (MQ=255)
                                                                                                                         cGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGt                        >  2:944133/1‑150 (MQ=255)
                                                                                                                         cGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGt                        >  1:1360901/1‑150 (MQ=255)
                                                                                                                         cGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGt                        >  2:4387385/1‑150 (MQ=255)
                                                                                                                            tGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTg                     <  2:5049517/150‑1 (MQ=255)
                                                                                                                            tGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTg                     >  2:2811615/1‑150 (MQ=255)
                                                                                                                            tGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTg                     >  2:4224374/1‑150 (MQ=255)
                                                                                                                             gTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTgg                    <  2:4538700/150‑1 (MQ=255)
                                                                                                                                cACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTg                 <  2:488396/150‑1 (MQ=255)
                                                                                                                                    tCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATa             <  1:555278/150‑1 (MQ=255)
                                                                                                                                     cAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATaa            <  1:3382916/150‑1 (MQ=255)
                                                                                                                                         ggTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaa        <  2:1080407/150‑1 (MQ=255)
                                                                                                                                         ggTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaa        <  2:1314227/150‑1 (MQ=255)
                                                                                                                                         ggTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaa        <  2:1570407/150‑1 (MQ=255)
                                                                                                                                            ttGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAcc     <  1:3607008/150‑1 (MQ=255)
                                                                                                                                            ttGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAcc     <  2:3963430/150‑1 (MQ=255)
                                                                                                                                            ttGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAcc     >  2:2974482/1‑150 (MQ=255)
                                                                                                                                             tGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCg    <  2:4796849/150‑1 (MQ=255)
                                                                                                                                               aaTTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTACACGCGGAAAAATCTGACGCGTCCAAAAGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGACACCGcc  >  2:4411531/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCC  >  CP009273/1003451‑1003743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAGT  >  CP009273/1003448‑1003746
                                                                                                                                                     |                                                                                                                                                     
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATT                                                                                                                                                       >  SRR19973063.916803/1‑150 (MQ=60)
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATT                                                                                                                                                       >  SRR19973063.1982468/1‑150 (MQ=60)
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATT                                                                                                                                                       >  SRR19973063.3072349/1‑150 (MQ=60)
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATT                                                                                                                                                       <  SRR19973063.3220781/150‑1 (MQ=60)
 GAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTA                                                                                                                                                      >  SRR19973063.4349838/1‑150 (MQ=60)
   GCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACA                                                                                                                                                    >  SRR19973063.3306405/1‑150 (MQ=60)
   GCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACA                                                                                                                                                    <  SRR19973063.2473834/150‑1 (MQ=60)
     CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACC                                                                                                                                                  <  SRR19973063.29474/150‑1 (MQ=60)
     CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACC                                                                                                                                                  <  SRR19973063.2307562/150‑1 (MQ=60)
     CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACC                                                                                                                                                  >  SRR19973063.2599870/1‑150 (MQ=60)
       CCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCAT                                                                                                                                                >  SRR19973063.1636435/1‑150 (MQ=60)
        CTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATC                                                                                                                                               >  SRR19973063.2617349/1‑150 (MQ=60)
         TGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCC                                                                                                                                              >  SRR19973063.4376224/1‑150 (MQ=60)
         TGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCC                                                                                                                                              <  SRR19973063.3735578/150‑1 (MQ=60)
            CCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCA                                                                                                                                           >  SRR19973063.153895/1‑150 (MQ=60)
             CTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAA                                                                                                                                          >  SRR19973063.4300385/1‑150 (MQ=60)
                GCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAG                                                                                                                                       <  SRR19973063.4941783/150‑1 (MQ=60)
                   TATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCA                                                                                                                                    <  SRR19973063.4870579/150‑1 (MQ=60)
                   TATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCA                                                                                                                                    <  SRR19973063.4111164/150‑1 (MQ=60)
                    ATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAG                                                                                                                                   >  SRR19973063.1575914/1‑150 (MQ=60)
                     TTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGT                                                                                                                                  <  SRR19973063.2391375/150‑1 (MQ=60)
                          TTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGC                                                                                                                             >  SRR19973063.5052413/1‑150 (MQ=60)
                             CCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCG                                                                                                                          <  SRR19973063.3062052/150‑1 (MQ=60)
                             CCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCG                                                                                                                          <  SRR19973063.104617/150‑1 (MQ=60)
                              CGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGA                                                                                                                         >  SRR19973063.1083691/1‑150 (MQ=60)
                              AGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGA                                                                                                                         >  SRR19973063.2064135/1‑150 (MQ=60)
                                CTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATG                                                                                                                       <  SRR19973063.2986466/150‑1 (MQ=60)
                                            AAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAC                                                                                                           >  SRR19973063.1574530/1‑150 (MQ=60)
                                             AGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACG                                                                                                          <  SRR19973063.1209635/150‑1 (MQ=60)
                                                TTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGA                                                                                                       >  SRR19973063.4664841/1‑150 (MQ=60)
                                                  TACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATC                                                                                                     <  SRR19973063.2511417/150‑1 (MQ=60)
                                                  TACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATC                                                                                                     >  SRR19973063.4323846/1‑150 (MQ=60)
                                                      GCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCG                                                                                                 >  SRR19973063.4553246/1‑150 (MQ=60)
                                                       CGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGA                                                                                                <  SRR19973063.3144634/150‑1 (MQ=60)
                                                         ATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATG                                                                                              >  SRR19973063.4062906/1‑150 (MQ=60)
                                                             AGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTT                                                                                          >  SRR19973063.2919331/1‑150 (MQ=60)
                                                              GACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTC                                                                                         <  SRR19973063.3727440/150‑1 (MQ=60)
                                                               ACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCA                                                                                        <  SRR19973063.4595483/150‑1 (MQ=60)
                                                               ACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCA                                                                                        <  SRR19973063.78219/150‑1 (MQ=60)
                                                                 CTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACG                                                                                      <  SRR19973063.1996534/150‑1 (MQ=60)
                                                                 CTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACG                                                                                      <  SRR19973063.1996681/150‑1 (MQ=60)
                                                                  TCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGC                                                                                     >  SRR19973063.2408987/1‑150 (MQ=60)
                                                                  TCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGC                                                                                     >  SRR19973063.3738059/1‑150 (MQ=60)
                                                                    TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                   <  SRR19973063.3239273/150‑1 (MQ=60)
                                                                    TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                   <  SRR19973063.3239277/150‑1 (MQ=60)
                                                                    TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                   >  SRR19973063.3394476/1‑150 (MQ=60)
                                                                    TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                   >  SRR19973063.1813377/1‑150 (MQ=60)
                                                                    TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                   <  SRR19973063.1930826/150‑1 (MQ=60)
                                                                      TCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAA                                                                                 <  SRR19973063.3067673/150‑1 (MQ=60)
                                                                       CTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAA                                                                                >  SRR19973063.392146/1‑150 (MQ=60)
                                                                       CTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAA                                                                                <  SRR19973063.4825453/150‑1 (MQ=60)
                                                                       CTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAA                                                                                >  SRR19973063.2095284/1‑150 (MQ=60)
                                                                          GGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAAT                                                                             >  SRR19973063.5065782/1‑150 (MQ=60)
                                                                           GTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATC                                                                            <  SRR19973063.543903/150‑1 (MQ=60)
                                                                           GTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAACC                                                                            <  SRR19973063.4920644/150‑1 (MQ=60)
                                                                              TTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGC                                                                         >  SRR19973063.3520510/1‑150 (MQ=60)
                                                                               TCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCC                                                                        <  SRR19973063.1963498/150‑1 (MQ=60)
                                                                               TCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCC                                                                        <  SRR19973063.4815558/150‑1 (MQ=60)
                                                                                CAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCG                                                                       >  SRR19973063.2743464/1‑150 (MQ=60)
                                                                                CAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCG                                                                       >  SRR19973063.2743670/1‑150 (MQ=60)
                                                                                 AGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGC                                                                      >  SRR19973063.486551/1‑150 (MQ=60)
                                                                                     GATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCC                                                                  >  SRR19973063.1186075/1‑150 (MQ=60)
                                                                                        CAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAA                                                               >  SRR19973063.3404122/1‑150 (MQ=60)
                                                                                        CAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAA                                                               >  SRR19973063.3585220/1‑150 (MQ=60)
                                                                                        CAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAA                                                               >  SRR19973063.3579488/1‑150 (MQ=60)
                                                                                           CTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGT                                                            >  SRR19973063.3687108/1‑150 (MQ=60)
                                                                                           CTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGT                                                            >  SRR19973063.3941751/1‑150 (MQ=60)
                                                                                            TGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTT                                                           <  SRR19973063.1716787/150‑1 (MQ=60)
                                                                                              GACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGA                                                         >  SRR19973063.1293710/1‑150 (MQ=60)
                                                                                               ACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAT                                                        >  SRR19973063.4496539/1‑150 (MQ=60)
                                                                                               ACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAT                                                        <  SRR19973063.4935076/150‑1 (MQ=60)
                                                                                               ACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAT                                                        <  SRR19973063.4941398/150‑1 (MQ=60)
                                                                                                CAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATC                                                       <  SRR19973063.940328/150‑1 (MQ=60)
                                                                                                CAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATC                                                       >  SRR19973063.3791273/1‑150 (MQ=60)
                                                                                                CAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATC                                                       >  SRR19973063.3791466/1‑150 (MQ=60)
                                                                                                CAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATC                                                       <  SRR19973063.940394/150‑1 (MQ=60)
                                                                                                 AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAATTCTGCCGCGTCCAAATGTTGATCG                                                      <  SRR19973063.175186/150‑1 (MQ=60)
                                                                                                 AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCG                                                      <  SRR19973063.4979899/150‑1 (MQ=60)
                                                                                                 AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCG                                                      <  SRR19973063.1749050/150‑1 (MQ=60)
                                                                                                 AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCG                                                      >  SRR19973063.119264/1‑150 (MQ=60)
                                                                                                 AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCG                                                      >  SRR19973063.3084075/1‑150 (MQ=60)
                                                                                                  GAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGC                                                     <  SRR19973063.2090580/150‑1 (MQ=60)
                                                                                                  GAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGC                                                     <  SRR19973063.4142429/150‑1 (MQ=60)
                                                                                                   AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                    <  SRR19973063.2972145/150‑1 (MQ=60)
                                                                                                    GATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGA                                                   <  SRR19973063.3454763/150‑1 (MQ=60)
                                                                                                     ATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGAT                                                  <  SRR19973063.2110790/150‑1 (MQ=60)
                                                                                                      TGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATG                                                 <  SRR19973063.4604265/150‑1 (MQ=60)
                                                                                                        TTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCG                                               <  SRR19973063.535577/150‑1 (MQ=60)
                                                                                                           AATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCG                                            <  SRR19973063.54759/150‑1 (MQ=60)
                                                                                                           AATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCG                                            <  SRR19973063.2362012/150‑1 (MQ=60)
                                                                                                            ATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGG                                           <  SRR19973063.2494365/150‑1 (MQ=60)
                                                                                                             TCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGA                                          <  SRR19973063.863469/150‑1 (MQ=60)
                                                                                                             TCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGA                                          <  SRR19973063.1191710/150‑1 (MQ=60)
                                                                                                              CCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                         >  SRR19973063.4415315/1‑150 (MQ=60)
                                                                                                              CCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                         >  SRR19973063.4400244/1‑150 (MQ=60)
                                                                                                              CCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                         >  SRR19973063.4870735/1‑150 (MQ=60)
                                                                                                               CTGGCGCGTCCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATA                                        >  SRR19973063.1643390/1‑150 (MQ=60)
                                                                                                                   CGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCG                                    >  SRR19973063.682973/1‑150 (MQ=60)
                                                                                                                   CGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCG                                    >  SRR19973063.682761/1‑150 (MQ=60)
                                                                                                                      GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                                 >  SRR19973063.212321/1‑150 (MQ=60)
                                                                                                                          TTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAAC                             >  SRR19973063.1753450/1‑150 (MQ=60)
                                                                                                                            CGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGT                           >  SRR19973063.1364992/1‑150 (MQ=60)
                                                                                                                            CGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGT                           <  SRR19973063.2729975/150‑1 (MQ=60)
                                                                                                                              CTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCT                         <  SRR19973063.2022784/150‑1 (MQ=60)
                                                                                                                                   CACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTG                    <  SRR19973063.4347355/150‑1 (MQ=60)
                                                                                                                                    ACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGC                   >  SRR19973063.5013507/1‑150 (MQ=60)
                                                                                                                                       TCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATA                <  SRR19973063.556920/150‑1 (MQ=60)
                                                                                                                                        CAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAA               <  SRR19973063.3393697/150‑1 (MQ=60)
                                                                                                                                         AGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAA              >  SRR19973063.3057669/1‑150 (MQ=60)
                                                                                                                                              TTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAC         <  SRR19973063.3340588/150‑1 (MQ=60)
                                                                                                                                              TTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAC         <  SRR19973063.3071398/150‑1 (MQ=60)
                                                                                                                                               TTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACC        <  SRR19973063.3618587/150‑1 (MQ=60)
                                                                                                                                                   ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGTAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAACTGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA    <  SRR19973063.2358915/150‑1 (MQ=60)
                                                                                                                                                   ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA    <  SRR19973063.645198/150‑1 (MQ=60)
                                                                                                                                                   ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA    <  SRR19973063.645144/150‑1 (MQ=60)
                                                                                                                                                   ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA    >  SRR19973063.2375945/1‑150 (MQ=60)
                                                                                                                                                   ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA    <  SRR19973063.4525154/150‑1 (MQ=60)
                                                                                                                                                   ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA    <  SRR19973063.4524893/150‑1 (MQ=60)
                                                                                                                                                    TTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAG   >  SRR19973063.651456/1‑150 (MQ=60)
                                                                                                                                                    TTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAG   <  SRR19973063.3567962/150‑1 (MQ=60)
                                                                                                                                                    TTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAG   <  SRR19973063.2891268/150‑1 (MQ=60)
                                                                                                                                                     TACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAGT  >  SRR19973063.5117719/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGAGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAGT  >  CP009273/1003448‑1003746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑