Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F527 I0 R1	1651	322.4	10661268	95.2%	10149527	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,003,597	G→T	100%	D100Y (GAC→TAC)	rlmL →	fused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,003,597	0	G	T	99.0%	926.4 / ‑0.1	286	D100Y (GAC→TAC)	rlmL	fused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (150/132); total (152/134)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 286 total aligned reads displayed.

AGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCC  >  CP009273/1003450‑1003743

aGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGac                                                                                                                                                  <  2:121366/150‑1 (MQ=255)

   cgccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAcc                                                                                                                                               >  2:5087587/1‑150 (MQ=255)

     ccTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCAt                                                                                                                                             >  1:5322695/1‑150 (MQ=255)

       tGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATcc                                                                                                                                           >  1:4227883/1‑150 (MQ=255)

          ccTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGAGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCa                                                                                                                                        >  2:327106/1‑150 (MQ=255)

           cTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCaa                                                                                                                                       <  2:4913003/150‑1 (MQ=255)

              gcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcag                                                                                                                                    >  2:2298280/1‑150 (MQ=255)

              gcgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcag                                                                                                                                    >  1:1636786/1‑150 (MQ=255)

               cgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagt                                                                                                                                   <  2:2552621/150‑1 (MQ=255)

               cgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagt                                                                                                                                   <  1:5165060/150‑1 (MQ=255)

               cgTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagt                                                                                                                                   <  2:3004864/150‑1 (MQ=255)

                gTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtc                                                                                                                                  >  1:3257370/1‑150 (MQ=255)

                gTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtc                                                                                                                                  >  1:4333659/1‑150 (MQ=255)

                  tttatGGTGCCGCTGGGCGAGTGTATGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCTGGCGATCTACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtcag                                                                                                                                <  1:2287626/149‑1 (MQ=255)

                   ttatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtcagt                                                                                                                               >  2:2331586/1‑150 (MQ=255)

                   ttatGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAcagtcagt                                                                                                                               >  1:4813321/1‑150 (MQ=255)

                     atGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTAc                                                                                                                             >  2:5182904/1‑150 (MQ=255)

                     atGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTAc                                                                                                                             <  2:2496632/150‑1 (MQ=255)

                           ccGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGcgcg                                                                                                                       >  1:3311095/1‑150 (MQ=255)

                              cTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtg                                                                                                                    <  1:4166765/150‑1 (MQ=255)

                                  gCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaag                                                                                                                <  1:4644604/150‑1 (MQ=255)

                                   cGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagt                                                                                                               <  1:4732514/150‑1 (MQ=255)

                                    gAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtg                                                                                                              >  2:1980605/1‑150 (MQ=255)

                                    gAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtg                                                                                                              >  2:2090369/1‑150 (MQ=255)

                                      gtgtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAtgaaagtgaa                                                                                                            >  2:1723213/1‑150 (MQ=255)

                                        gtAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATCTTTAATCCTGGCGCGACCTTCGCGGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGAACGGCGCGAtgaaagtgaaag                                                                                                          >  1:1114891/1‑150 (MQ=255)

                                          aaGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAc                                                                                                        <  2:4017361/150‑1 (MQ=255)

                                           aGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGANAGTGAAAGAcg                                                                                                       <  1:2760915/150‑1 (MQ=255)

                                             gTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAcgcg                                                                                                     >  1:1692460/1‑150 (MQ=255)

                                             gTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAcgcg                                                                                                     <  2:4582557/150‑1 (MQ=255)

                                             gTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAcgcg                                                                                                     >  1:2462764/1‑150 (MQ=255)

                                              tttACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGa                                                                                                    >  2:1661672/1‑150 (MQ=255)

                                                tACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtc                                                                                                  <  1:2843277/150‑1 (MQ=255)

                                                   aGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtc                                                                                               >  2:4706053/1‑150 (MQ=255)

                                                   aGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtc                                                                                               <  1:3155529/150‑1 (MQ=255)

                                                   aGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtc                                                                                               <  2:285784/150‑1 (MQ=255)

                                                   aGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtc                                                                                               <  2:1070910/150‑1 (MQ=255)

                                                    gCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtcg                                                                                              <  1:603608/150‑1 (MQ=255)

                                                    gCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtcg                                                                                              <  1:897286/150‑1 (MQ=255)

                                                    gCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGAtcgtcg                                                                                              <  2:4819931/150‑1 (MQ=255)

                                                      gATTTAGACCTCTATCTCGGTGTTCAGGAGATCAAATGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCATTGGTTTGAATTACACCATCCGCAACAGTCTGTACGGGGCGATGAAAGTGAAAGACGCGATCGTCGAt                                                                                            <  1:1144903/150‑1 (MQ=255)

                                                          tAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCtt                                                                                        <  1:286953/150‑1 (MQ=255)

                                                          tAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCtt                                                                                        <  1:265177/150‑1 (MQ=255)

                                                            gACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTc                                                                                      <  2:1595241/150‑1 (MQ=255)

                                                            gACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTc                                                                                      >  2:3365245/1‑150 (MQ=255)

                                                             aCCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGACAGTGAAAGACGCGATCGTCGATGCTTTCa                                                                                     <  2:757321/150‑1 (MQ=255)

                                                             aCCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCa                                                                                     <  2:3153438/150‑1 (MQ=255)

                                                             aCCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCa                                                                                     <  2:2776271/150‑1 (MQ=255)

                                                              ccTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAc                                                                                    >  1:5099436/1‑150 (MQ=255)

                                                                    tctcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaa                                                                              <  2:3489678/150‑1 (MQ=255)

                                                                    tctcGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCATGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCTACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaa                                                                              >  2:578027/1‑150 (MQ=255)

                                                                       cGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGacaaa                                                                           >  2:3094024/1‑150 (MQ=255)

                                                                       cGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGaaaaa                                                                           <  1:104733/150‑1 (MQ=255)

                                                                        ggTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAAt                                                                          >  1:5231865/1‑150 (MQ=255)

                                                                          tgtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCt                                                                        <  1:2588322/150‑1 (MQ=255)

                                                                          tgtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAACATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCt                                                                        >  2:1457852/1‑150 (MQ=255)

                                                                           gtTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTg                                                                       >  1:4567056/1‑150 (MQ=255)

                                                                             tCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGcc                                                                     <  1:1510385/150‑1 (MQ=255)

                                                                              cAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcg                                                                    >  2:101519/1‑150 (MQ=255)

                                                                               aGGCGATCAACTGGACAGAGATGTTTAATCCTGTCGCGACCTTCGCTGTCCACTTCAGTTGTTTGAATTACACCATCCGCAACAGTCAGTAAGTCGAGATGAAAGTGAAAGACTCGATAGTCGATTCTTTCACGCGTAAAAATCTGCcgc                                                                   >  2:1270572/1‑150 (MQ=255)

                                                                               aGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTATGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCcgc                                                                   >  2:2409675/1‑150 (MQ=255)

                                                                                   gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               >  2:4604030/1‑150 (MQ=255)

                                                                                   gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               >  2:942378/1‑150 (MQ=255)

                                                                                   gATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTcc                                                               >  1:3891186/1‑150 (MQ=255)

                                                                                    aTCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCa                                                              <  1:3423932/150‑1 (MQ=255)

                                                                                     tCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCaa                                                             >  1:1524711/1‑150 (MQ=255)

                                                                                       aaCTGGACAGAGAGGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCTACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAAt                                                           <  2:4773164/150‑1 (MQ=255)

                                                                                        aCTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATg                                                          >  2:4629777/1‑150 (MQ=255)

                                                                                           ggACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTg                                                       >  1:3711786/1‑150 (MQ=255)

                                                                                             aCAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAt                                                     <  2:3822006/150‑1 (MQ=255)

                                                                                              cAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATc                                                    <  1:2367603/150‑1 (MQ=255)

                                                                                               agagATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcg                                                   <  2:2588868/150‑1 (MQ=255)

                                                                                               agagATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcg                                                   <  1:3001115/150‑1 (MQ=255)

                                                                                               agagATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcg                                                   >  2:34314/1‑150 (MQ=255)

                                                                                                gagaTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATcgc                                                  >  2:3651322/1‑150 (MQ=255)

                                                                                                   aTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGAt                                               >  1:3959549/1‑150 (MQ=255)

                                                                                                   aTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGAt                                               <  2:5023189/150‑1 (MQ=255)

                                                                                                   aTGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGAt                                               >  2:3524274/1‑150 (MQ=255)

                                                                                                     gTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATgc                                             >  1:2267387/1‑150 (MQ=255)

                                                                                                         aaTCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCg                                         <  2:5313748/150‑1 (MQ=255)

                                                                                                            ccTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGat                                      >  1:488776/1‑150 (MQ=255)

                                                                                                            ccTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGat                                      >  1:753232/1‑150 (MQ=255)

                                                                                                                gcgcGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATcc                                  <  1:426820/150‑1 (MQ=255)

                                                                                                                gcgcGACCTTCGCTGTCCACTTCAGTGGTTTGAAGTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATcc                                  <  2:4390039/150‑1 (MQ=255)

                                                                                                                    gACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGt                              <  2:1530108/150‑1 (MQ=255)

                                                                                                                       cTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTaa                           >  1:3541351/1‑150 (MQ=255)

                                                                                                                        ttCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAAc                          <  2:4059657/150‑1 (MQ=255)

                                                                                                                        ttCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAAc                          <  1:1272561/150‑1 (MQ=255)

                                                                                                                         tCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACg                         >  2:1239563/1‑150 (MQ=255)

                                                                                                                         tCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACg                         >  2:861261/1‑150 (MQ=255)

                                                                                                                            cTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCt                      >  1:4531091/1‑150 (MQ=255)

                                                                                                                                  aCTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGc                >  2:1146782/1‑150 (MQ=255)

                                                                                                                                    ttCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCAt              <  1:2090369/150‑1 (MQ=255)

                                                                                                                                      cAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCTCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATaa            >  2:2709366/1‑150 (MQ=255)

                                                                                                                                          ggTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaa        <  2:2751589/150‑1 (MQ=255)

                                                                                                                                          ggTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGaa        >  1:141754/1‑150 (MQ=255)

                                                                                                                                            tttGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAc      <  1:3576411/150‑1 (MQ=255)

                                                                                                                                              tGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCg    <  2:2217063/150‑1 (MQ=255)

                                                                                                                                               gAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGTCTGCATAAAGAAACCGc   >  2:3540041/1‑150 (MQ=255)

                                                                                                                                               gAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGc   <  2:4606569/150‑1 (MQ=255)

AGCCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCC  >  CP009273/1003450‑1003743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA  >  CP009273/1003452‑1003744

CCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACAC                                                                                                                                                 >  SRR19973062.472817/1‑150 (MQ=60)

 CGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACC                                                                                                                                                <  SRR19973062.1257729/150‑1 (MQ=60)

  GCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCA                                                                                                                                               <  SRR19973062.72271/150‑1 (MQ=60)

   CCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCAT                                                                                                                                              >  SRR19973062.5350619/1‑150 (MQ=60)

     TGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCC                                                                                                                                            <  SRR19973062.2844656/150‑1 (MQ=60)

     TGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCC                                                                                                                                            >  SRR19973062.4249789/1‑150 (MQ=60)

        CCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCA                                                                                                                                         >  SRR19973062.2565207/1‑150 (MQ=60)

          TCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAAC                                                                                                                                       >  SRR19973062.2766114/1‑150 (MQ=60)

            GCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAG                                                                                                                                     >  SRR19973062.3727329/1‑150 (MQ=60)

            GCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAG                                                                                                                                     >  SRR19973062.1644857/1‑150 (MQ=60)

             CGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGT                                                                                                                                    <  SRR19973062.1068241/150‑1 (MQ=60)

             CGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGT                                                                                                                                    <  SRR19973062.2534283/150‑1 (MQ=60)

             CGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGT                                                                                                                                    <  SRR19973062.4831674/150‑1 (MQ=60)

             CGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGT                                                                                                                                    <  SRR19973062.5192349/150‑1 (MQ=60)

              GTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTC                                                                                                                                   >  SRR19973062.3274253/1‑150 (MQ=60)

              GTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTC                                                                                                                                   >  SRR19973062.4355971/1‑150 (MQ=60)

              GTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTC                                                                                                                                   >  SRR19973062.3274272/1‑150 (MQ=60)

              GTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTC                                                                                                                                   >  SRR19973062.2712852/1‑150 (MQ=60)

                TTTATGGTGCCGCTGGGCGAGTGTATGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCTGGCGATCTACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAG                                                                                                                                 <  SRR19973062.2299459/150‑1 (MQ=60)

                ATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACGGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAG                                                                                                                                 >  SRR19973062.3879403/1‑150 (MQ=60)

                ATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAG                                                                                                                                 >  SRR19973062.4368915/1‑150 (MQ=60)

                 TTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGT                                                                                                                                >  SRR19973062.4838258/1‑150 (MQ=60)

                 TTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGT                                                                                                                                <  SRR19973062.1568983/150‑1 (MQ=60)

                  TATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTA                                                                                                                               >  SRR19973062.1181806/1‑150 (MQ=60)

                   ATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTAC                                                                                                                              <  SRR19973062.1634082/150‑1 (MQ=60)

                    TGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACG                                                                                                                             >  SRR19973062.1974267/1‑150 (MQ=60)

                    TGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACG                                                                                                                             <  SRR19973062.825551/150‑1 (MQ=60)

                         CCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCG                                                                                                                        >  SRR19973062.3328218/1‑150 (MQ=60)

                           GCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGAT                                                                                                                      <  SRR19973062.1525907/150‑1 (MQ=60)

                            CTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATG                                                                                                                     <  SRR19973062.4188388/150‑1 (MQ=60)

                             TGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGA                                                                                                                    >  SRR19973062.4852845/1‑150 (MQ=60)

                               GNCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAA                                                                                                                  >  SRR19973062.3163438/1‑150 (MQ=60)

                                GCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAG                                                                                                                 <  SRR19973062.4668906/150‑1 (MQ=60)

                                 CGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGT                                                                                                                <  SRR19973062.4757194/150‑1 (MQ=60)

                                      GTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATCTTTAATCCTGGCGCGACCTTCGCGGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGAACGGCGCGATGAAAGTGAAAG                                                                                                           >  SRR19973062.1120123/1‑150 (MQ=60)

                                        AAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAC                                                                                                         <  SRR19973062.5202291/150‑1 (MQ=60)

                                        AAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGAC                                                                                                         <  SRR19973062.2949543/150‑1 (MQ=60)

                                         AGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGANAGTGAAAGACG                                                                                                        <  SRR19973062.2775499/150‑1 (MQ=60)

                                         AGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACG                                                                                                        <  SRR19973062.233958/150‑1 (MQ=60)

                                         AGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACG                                                                                                        <  SRR19973062.2660806/150‑1 (MQ=60)

                                           GTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCG                                                                                                      >  SRR19973062.2475968/1‑150 (MQ=60)

                                           GTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCG                                                                                                      >  SRR19973062.3550474/1‑150 (MQ=60)

                                           GTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCG                                                                                                      >  SRR19973062.5049793/1‑150 (MQ=60)

                                           GTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCG                                                                                                      >  SRR19973062.1700758/1‑150 (MQ=60)

                                           GTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCG                                                                                                      >  SRR19973062.5135203/1‑150 (MQ=60)

                                              TACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATC                                                                                                   <  SRR19973062.1296609/150‑1 (MQ=60)

                                              TACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATC                                                                                                   <  SRR19973062.2858315/150‑1 (MQ=60)

                                                 AGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTC                                                                                                <  SRR19973062.1146246/150‑1 (MQ=60)

                                                 AGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTC                                                                                                <  SRR19973062.3171835/150‑1 (MQ=60)

                                                 AGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTC                                                                                                >  SRR19973062.2985358/1‑150 (MQ=60)

                                                 AGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAACACGCGATCGTC                                                                                                <  SRR19973062.3219002/150‑1 (MQ=60)

                                                  GCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCG                                                                                               <  SRR19973062.901120/150‑1 (MQ=60)

                                                  GCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCG                                                                                               <  SRR19973062.606431/150‑1 (MQ=60)

                                                   CGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGACAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGA                                                                                              >  SRR19973062.2799665/1‑150 (MQ=60)

                                                    GATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGAT                                                                                             >  SRR19973062.5172051/1‑150 (MQ=60)

                                                    GATTTAGACCTCTATCTCGGTGTTCAGGAGATCAAATGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCATTGGTTTGAATTACACCATCCGCAACAGTCTGTACGGGGCGATGAAAGTGAAAGACGCGATCGTCGAT                                                                                             <  SRR19973062.1150269/150‑1 (MQ=60)

                                                        TAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTT                                                                                         <  SRR19973062.288356/150‑1 (MQ=60)

                                                        TAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTT                                                                                         <  SRR19973062.266503/150‑1 (MQ=60)

                                                        TAGACATCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTT                                                                                         <  SRR19973062.511607/150‑1 (MQ=60)

                                                         AGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTT                                                                                        >  SRR19973062.1524218/1‑150 (MQ=60)

                                                          GACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTC                                                                                       >  SRR19973062.980011/1‑150 (MQ=60)

                                                          GACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTC                                                                                       >  SRR19973062.2865528/1‑150 (MQ=60)

                                                          GACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTC                                                                                       >  SRR19973062.2865747/1‑150 (MQ=60)

                                                           ACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCA                                                                                      >  SRR19973062.94150/1‑150 (MQ=60)

                                                            CCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAC                                                                                     <  SRR19973062.4616356/150‑1 (MQ=60)

                                                            CCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCAC                                                                                     >  SRR19973062.5126434/1‑150 (MQ=60)

                                                                TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                 >  SRR19973062.4300094/1‑150 (MQ=60)

                                                                TATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGG                                                                                 >  SRR19973062.2131258/1‑150 (MQ=60)

                                                                 ATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGA                                                                                >  SRR19973062.5291479/1‑150 (MQ=60)

                                                                     CGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAA                                                                            <  SRR19973062.105402/150‑1 (MQ=60)

                                                                      GGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAAT                                                                           >  SRR19973062.5259434/1‑150 (MQ=60)

                                                                       GTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATC                                                                          >  SRR19973062.3368724/1‑150 (MQ=60)

                                                                        TGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCT                                                                         <  SRR19973062.2602045/150‑1 (MQ=60)

                                                                         GTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTG                                                                        >  SRR19973062.4590911/1‑150 (MQ=60)

                                                                           TCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCC                                                                      <  SRR19973062.791591/150‑1 (MQ=60)

                                                                           TCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCC                                                                      <  SRR19973062.1517829/150‑1 (MQ=60)

                                                                                 GATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCC                                                                >  SRR19973062.3911016/1‑150 (MQ=60)

                                                                                 GATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCC                                                                <  SRR19973062.3933427/150‑1 (MQ=60)

                                                                                  ATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCA                                                               <  SRR19973062.3441445/150‑1 (MQ=60)

                                                                                  ATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCA                                                               <  SRR19973062.3441471/150‑1 (MQ=60)

                                                                                   TCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAA                                                              >  SRR19973062.1532225/1‑150 (MQ=60)

                                                                                   TCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAA                                                              >  SRR19973062.3390029/1‑150 (MQ=60)

                                                                                    CAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAA                                                             >  SRR19973062.5316162/1‑150 (MQ=60)

                                                                                    CAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAA                                                             <  SRR19973062.4611213/150‑1 (MQ=60)

                                                                                     AACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAAT                                                            >  SRR19973062.4277373/1‑150 (MQ=60)

                                                                                         GGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTG                                                        >  SRR19973062.3730839/1‑150 (MQ=60)

                                                                                         GGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTG                                                        >  SRR19973062.3960486/1‑150 (MQ=60)

                                                                                           ACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGAT                                                      <  SRR19973062.2269815/150‑1 (MQ=60)

                                                                                            CAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATC                                                     <  SRR19973062.1288234/150‑1 (MQ=60)

                                                                                            CAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATC                                                     <  SRR19973062.2380274/150‑1 (MQ=60)

                                                                                             AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCG                                                    >  SRR19973062.4568533/1‑150 (MQ=60)

                                                                                             AGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCG                                                    <  SRR19973062.3016811/150‑1 (MQ=60)

                                                                                               AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                  >  SRR19973062.3286958/1‑150 (MQ=60)

                                                                                               AGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCAGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCG                                                  <  SRR19973062.3605572/150‑1 (MQ=60)

                                                                                                GATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGGTGGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGGGACAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTGCGAAGGTTGATCGCGA                                                 >  SRR19973062.4344609/1‑150 (MQ=60)

                                                                                                 ATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGAT                                                >  SRR19973062.3979632/1‑150 (MQ=60)

                                                                                                 ATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGAT                                                >  SRR19973062.4217324/1‑150 (MQ=60)

                                                                                                  TGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATG                                               <  SRR19973062.2414549/150‑1 (MQ=60)

                                                                                                  TGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATG                                               <  SRR19973062.3569752/150‑1 (MQ=60)

                                                                                                   GTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGC                                              <  SRR19973062.1478479/150‑1 (MQ=60)

                                                                                                   GTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGC                                              >  SRR19973062.2278910/1‑150 (MQ=60)

                                                                                                     TTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGC                                            <  SRR19973062.2298227/150‑1 (MQ=60)

                                                                                                          CCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                       >  SRR19973062.756640/1‑150 (MQ=60)

                                                                                                          CCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                       >  SRR19973062.5214162/1‑150 (MQ=60)

                                                                                                          CCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                       >  SRR19973062.490920/1‑150 (MQ=60)

                                                                                                          CCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGAT                                       >  SRR19973062.491031/1‑150 (MQ=60)

                                                                                                              GCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCC                                   <  SRR19973062.428651/150‑1 (MQ=60)

                                                                                                              GCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCC                                   <  SRR19973062.428620/150‑1 (MQ=60)

                                                                                                                  GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                               >  SRR19973062.72175/1‑150 (MQ=60)

                                                                                                                  GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                               >  SRR19973062.4476556/1‑150 (MQ=60)

                                                                                                                  GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                               >  SRR19973062.4788992/1‑150 (MQ=60)

                                                                                                                  GACCTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGT                               >  SRR19973062.3019398/1‑150 (MQ=60)

                                                                                                                     CTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAA                            <  SRR19973062.4064141/150‑1 (MQ=60)

                                                                                                                     CTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAA                            >  SRR19973062.3559250/1‑150 (MQ=60)

                                                                                                                     CTTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAA                            >  SRR19973062.3559245/1‑150 (MQ=60)

                                                                                                                      TTCGCTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAAC                           <  SRR19973062.1278425/150‑1 (MQ=60)

                                                                                                                          CTGTCCACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCT                       >  SRR19973062.4554664/1‑150 (MQ=60)

                                                                                                                                ACTTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGC                 <  SRR19973062.1669842/150‑1 (MQ=60)

                                                                                                                                  TTCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCAT               <  SRR19973062.2101051/150‑1 (MQ=60)

                                                                                                                                   TCAGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATA              <  SRR19973062.4586118/150‑1 (MQ=60)

                                                                                                                                     AGTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAA            >  SRR19973062.3908543/1‑150 (MQ=60)

                                                                                                                                      GTGGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATTCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAG           <  SRR19973062.1938493/150‑1 (MQ=60)

                                                                                                                                        GGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAA         >  SRR19973062.142593/1‑150 (MQ=60)

                                                                                                                                        GGTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAA         >  SRR19973062.142590/1‑150 (MQ=60)

                                                                                                                                         GTTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAA        >  SRR19973062.4222160/1‑150 (MQ=60)

                                                                                                                                          TTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAC       <  SRR19973062.3594470/150‑1 (MQ=60)

                                                                                                                                          TTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAC       >  SRR19973062.2285444/1‑150 (MQ=60)

                                                                                                                                          TTTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAAC       <  SRR19973062.2448247/150‑1 (MQ=60)

                                                                                                                                           TTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACC      <  SRR19973062.535674/150‑1 (MQ=60)

                                                                                                                                           TTGAATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACC      <  SRR19973062.2310243/150‑1 (MQ=60)

                                                                                                                                              AATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCC   >  SRR19973062.1140265/1‑150 (MQ=60)

                                                                                                                                               ATTACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA  >  SRR19973062.3183344/1‑150 (MQ=60)

CCGCCTGGCCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAACTGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTACGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATGCGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCA  >  CP009273/1003452‑1003744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`