Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,886,725 G→T I256I (ATC→ATA)  yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,886,7250GT99.5% 709.7 / ‑4.9 213I256I (ATC→ATA) yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (108/104);  minor base A (1/0);  total (109/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 213 total aligned reads displayed.

AATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT  >  CP009273/1886581‑1886870
                                                                                                                                                |                                                                                                                                                 
aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATgat                                                                                                                                              >  1:1791534/1‑148 (MQ=255)
aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGt                                                                                                                                              >  2:2412756/1‑150 (MQ=255)
    tGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATg                                                                                                                                          <  1:699319/150‑1 (MQ=255)
      cGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTc                                                                                                                                        >  2:591625/1‑150 (MQ=255)
       ggATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCt                                                                                                                                       >  1:292439/1‑150 (MQ=255)
       ggATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCt                                                                                                                                       >  2:131360/1‑150 (MQ=255)
       ggATTAGCTAACAGCTCACGCAGGTGACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCt                                                                                                                                       <  2:121210/150‑1 (MQ=255)
        gATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCtt                                                                                                                                      >  2:1360895/1‑150 (MQ=255)
           tAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTg                                                                                                                                   <  2:2329616/150‑1 (MQ=255)
               tAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAg                                                                                                                               <  2:2909184/150‑1 (MQ=255)
                aaCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAgg                                                                                                                              <  2:1550927/150‑1 (MQ=255)
                aaCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAgg                                                                                                                              >  2:1208516/1‑150 (MQ=255)
                  cAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTc                                                                                                                            <  2:3555633/150‑1 (MQ=255)
                  cAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTc                                                                                                                            <  2:1177936/150‑1 (MQ=255)
                   aGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCg                                                                                                                           <  1:1985270/150‑1 (MQ=255)
                    gCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGa                                                                                                                          <  2:2082253/150‑1 (MQ=255)
                    gCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGa                                                                                                                          <  2:3374435/150‑1 (MQ=255)
                     cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAg                                                                                                                         >  1:1405539/1‑150 (MQ=255)
                          gCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGtt                                                                                                                    <  1:525716/150‑1 (MQ=255)
                           cAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTg                                                                                                                   >  1:1545031/1‑150 (MQ=255)
                            aGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGt                                                                                                                  <  2:3402817/150‑1 (MQ=255)
                                tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGAc                                                                                                              >  2:76275/1‑150 (MQ=255)
                                tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGAc                                                                                                              >  1:1205406/1‑150 (MQ=255)
                                  ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTg                                                                                                            <  2:3113872/150‑1 (MQ=255)
                                  ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTg                                                                                                            <  2:3012581/150‑1 (MQ=255)
                                    gcgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGga                                                                                                          <  2:2417962/150‑1 (MQ=255)
                                     cgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGgag                                                                                                         >  1:3362968/1‑150 (MQ=255)
                                     cgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGgag                                                                                                         >  2:803344/1‑150 (MQ=255)
                                     cgATAACCTGGCTCACCGAGTTGCAGACGAAAATCATGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGgag                                                                                                         >  2:385989/1‑150 (MQ=255)
                                      gATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGgaga                                                                                                        >  2:740058/1‑150 (MQ=255)
                                            cTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgact                                                                                                  <  1:3551664/150‑1 (MQ=255)
                                            cTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgact                                                                                                  <  1:722309/150‑1 (MQ=255)
                                             tGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgactg                                                                                                 >  2:1839152/1‑150 (MQ=255)
                                                 tCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCa                                                                                             >  1:1574463/1‑150 (MQ=255)
                                                     cGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAAt                                                                                         >  2:274821/1‑150 (MQ=255)
                                                        gTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATAct                                                                                      <  1:3240111/150‑1 (MQ=255)
                                                         ttGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActg                                                                                     <  2:3046435/150‑1 (MQ=255)
                                                          tGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActgg                                                                                    >  1:1646611/1‑150 (MQ=255)
                                                            cAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggct                                                                                  >  1:2597444/1‑150 (MQ=255)
                                                             aGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggctg                                                                                 >  2:1956720/1‑150 (MQ=255)
                                                              gACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTATAGAAACTGCTGGGTGTCTTTTAATACTTTACTGTAGACTATTGTGATGTCTTTTACCAGGTCTAGCAGTAGTCGACTGTAGAGTGACTTACCAAAATActaactgt                                                                                >  1:394584/1‑144 (MQ=255)
                                                                cGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCa                                                                              >  1:742099/1‑150 (MQ=255)
                                                                 gAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCaa                                                                             >  2:2591701/1‑150 (MQ=255)
                                                                  aaaaTCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAAt                                                                            >  2:3514651/1‑150 (MQ=255)
                                                                     aTCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTc                                                                         >  2:2719288/1‑150 (MQ=255)
                                                                     aTCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTc                                                                         <  2:3230466/150‑1 (MQ=255)
                                                                      tCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTcc                                                                        >  1:676897/1‑150 (MQ=255)
                                                                          gcgcACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTa                                                                    <  2:1717034/150‑1 (MQ=255)
                                                                           cgcACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAg                                                                   >  2:3658611/1‑150 (MQ=255)
                                                                           cgcACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGAAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAg                                                                   >  1:1249603/1‑150 (MQ=255)
                                                                             cACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCt                                                                 >  1:2384523/1‑150 (MQ=255)
                                                                             cACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCt                                                                 <  1:1703243/150‑1 (MQ=255)
                                                                             cACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCt                                                                 >  2:3625597/1‑150 (MQ=255)
                                                                             cACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCt                                                                 <  1:1208516/150‑1 (MQ=255)
                                                                              aCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtg                                                                >  2:3694489/1‑150 (MQ=255)
                                                                                 ctGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGGGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtggtg                                                             >  2:409952/1‑150 (MQ=255)
                                                                                 ctGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtggtg                                                             >  2:2317633/1‑150 (MQ=255)
                                                                                 ctGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtggtg                                                             >  2:21284/1‑150 (MQ=255)
                                                                                 ctGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtggtg                                                             <  2:2955581/150‑1 (MQ=255)
                                                                                  tGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtggtgg                                                            <  2:1056006/150‑1 (MQ=255)
                                                                                   gggCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTggg                                                           >  2:1751463/1‑150 (MQ=255)
                                                                                   gggCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTggg                                                           >  2:2815021/1‑150 (MQ=255)
                                                                                    ggCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGc                                                          <  1:2831960/150‑1 (MQ=255)
                                                                                     gCAAGACGATCAGCGCACTTGTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCt                                                         <  1:3432429/150‑1 (MQ=255)
                                                                                      cAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCtt                                                        >  1:2563736/1‑150 (MQ=255)
                                                                                        aGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTtcg                                                      >  2:3363838/1‑150 (MQ=255)
                                                                                            gATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGa                                                  >  2:1267505/1‑150 (MQ=255)
                                                                                            gATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGa                                                  <  1:2801653/150‑1 (MQ=255)
                                                                                                aGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGAt                                              <  2:2710008/150‑1 (MQ=255)
                                                                                                aGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGAt                                              >  1:1009976/1‑150 (MQ=255)
                                                                                                 gcgcACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATc                                             <  1:2727864/150‑1 (MQ=255)
                                                                                                 gcgcACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATc                                             <  2:833533/150‑1 (MQ=255)
                                                                                                    cACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATg                                          >  2:3151076/1‑150 (MQ=255)
                                                                                                    cACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATg                                          >  1:1154250/1‑150 (MQ=255)
                                                                                                       ttttGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACg                                       <  1:3088971/150‑1 (MQ=255)
                                                                                                          tGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTcc                                    <  2:3073439/150‑1 (MQ=255)
                                                                                                           gTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCg                                   <  2:292439/150‑1 (MQ=255)
                                                                                                           gTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCg                                   >  2:1529242/1‑150 (MQ=255)
                                                                                                            tAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGc                                  >  1:2950295/1‑150 (MQ=255)
                                                                                                              aCTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCtt                                <  2:382836/150‑1 (MQ=255)
                                                                                                               ctgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTc                               >  2:1942513/1‑150 (MQ=255)
                                                                                                               ctgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTc                               >  2:2287125/1‑150 (MQ=255)
                                                                                                                tgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTcc                              <  1:1819351/150‑1 (MQ=255)
                                                                                                                tgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTcc                              <  2:1205403/150‑1 (MQ=255)
                                                                                                                tgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTcc                              >  1:1316435/1‑150 (MQ=255)
                                                                                                                  ctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCgg                            >  1:686635/1‑150 (MQ=255)
                                                                                                                       tgtCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCa                       <  1:1524212/150‑1 (MQ=255)
                                                                                                                        gtCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAg                      <  2:1801678/150‑1 (MQ=255)
                                                                                                                          cTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGtt                    <  2:344685/150‑1 (MQ=255)
                                                                                                                          cTTTTAATTCGGTGCGGGAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGtt                    <  2:3343750/150‑1 (MQ=255)
                                                                                                                           ttttAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTc                   <  2:2470638/150‑1 (MQ=255)
                                                                                                                            tttAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCg                  >  1:2176028/1‑150 (MQ=255)
                                                                                                                               aaTTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCa               >  2:725366/1‑150 (MQ=255)
                                                                                                                                   cGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCACTGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATc           >  2:1162215/1‑150 (MQ=255)
                                                                                                                                   cGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATc           >  2:205039/1‑150 (MQ=255)
                                                                                                                                    ggTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATcc          >  2:3172498/1‑150 (MQ=255)
                                                                                                                                       gCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCAct       <  2:1646611/150‑1 (MQ=255)
                                                                                                                                         ggTAGGCTATGGTGATGTTTTTTGCCAGGTCGAGCTGTTGTCGATTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActct     <  1:385989/150‑1 (MQ=255)
                                                                                                                                            aGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCtt  <  1:3596071/150‑1 (MQ=255)
                                                                                                                                            aGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCtt  >  1:3519358/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
AATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT  >  CP009273/1886581‑1886870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAAC  >  CP009273/1886581‑1886874
                                                                                                                                                |                                                                                                                                                     
AATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGAT                                                                                                                                                  >  SRR19973061.1796018/1‑150 (MQ=60)
   TTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGAT                                                                                                                                               <  SRR19973061.1426518/150‑1 (MQ=60)
    TGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATG                                                                                                                                              <  SRR19973061.701141/150‑1 (MQ=60)
    TGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATG                                                                                                                                              <  SRR19973061.701110/150‑1 (MQ=60)
    TGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATG                                                                                                                                              >  SRR19973061.3081372/1‑150 (MQ=60)
       GGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCT                                                                                                                                           >  SRR19973061.293222/1‑150 (MQ=60)
          TTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTT                                                                                                                                        <  SRR19973061.2348088/150‑1 (MQ=60)
           TAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTG                                                                                                                                       <  SRR19973061.1547127/150‑1 (MQ=60)
            AGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGC                                                                                                                                      >  SRR19973061.835706/1‑150 (MQ=60)
              CTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCA                                                                                                                                    >  SRR19973061.3523042/1‑150 (MQ=60)
                 ACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGT                                                                                                                                 >  SRR19973061.2658014/1‑150 (MQ=60)
                   AGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCG                                                                                                                               <  SRR19973061.1990238/150‑1 (MQ=60)
                     CTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAG                                                                                                                             >  SRR19973061.1409085/1‑150 (MQ=60)
                         CGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGT                                                                                                                         <  SRR19973061.157838/150‑1 (MQ=60)
                          GCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTT                                                                                                                        <  SRR19973061.527105/150‑1 (MQ=60)
                           CAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTG                                                                                                                       >  SRR19973061.1548877/1‑150 (MQ=60)
                            AGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGT                                                                                                                      >  SRR19973061.1058719/1‑150 (MQ=60)
                                TACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGAC                                                                                                                  >  SRR19973061.1208432/1‑150 (MQ=60)
                                    GCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGA                                                                                                              >  SRR19973061.741782/1‑150 (MQ=60)
                                     CGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAG                                                                                                             >  SRR19973061.3371616/1‑150 (MQ=60)
                                     CGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAG                                                                                                             >  SRR19973061.1703067/1‑150 (MQ=60)
                                            CTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACT                                                                                                      <  SRR19973061.3560737/150‑1 (MQ=60)
                                            CTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACT                                                                                                      <  SRR19973061.3560756/150‑1 (MQ=60)
                                            CTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACT                                                                                                      <  SRR19973061.724196/150‑1 (MQ=60)
                                             TGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTG                                                                                                     <  SRR19973061.2407540/150‑1 (MQ=60)
                                                 TCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCA                                                                                                 >  SRR19973061.1578375/1‑150 (MQ=60)
                                                 TCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCA                                                                                                 <  SRR19973061.1364327/150‑1 (MQ=60)
                                                        GTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACT                                                                                          <  SRR19973061.3248449/150‑1 (MQ=60)
                                                          TGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGG                                                                                        >  SRR19973061.1650747/1‑150 (MQ=60)
                                                            CAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCT                                                                                      >  SRR19973061.2604109/1‑150 (MQ=60)
                                                              GACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGG                                                                                    >  SRR19973061.238857/1‑150 (MQ=60)
                                                              GACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGG                                                                                    <  SRR19973061.2750837/150‑1 (MQ=60)
                                                              GACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCAATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGG                                                                                    >  SRR19973061.395104/1‑150 (MQ=60)
                                                              GACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTATAGAAACTGCTGGGTGTCTTTTAATACTTTACTGTAGACTATTGTGATGTCTTTTACCAGGTCTAGCAGTAGTCGACTGTAGAGTGACTTACCAAAATACTaactgt                                                                                    >  SRR19973061.395658/1‑144 (MQ=60)
                                                                CGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCA                                                                                  >  SRR19973061.744043/1‑150 (MQ=60)
                                                                      TCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCC                                                                            >  SRR19973061.678653/1‑150 (MQ=60)
                                                                        CTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGG                                                                          <  SRR19973061.526643/150‑1 (MQ=60)
                                                                           CGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGAAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAG                                                                       >  SRR19973061.1252699/1‑150 (MQ=60)
                                                                             CACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCT                                                                     <  SRR19973061.1211548/150‑1 (MQ=60)
                                                                             CACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCT                                                                     <  SRR19973061.1210679/150‑1 (MQ=60)
                                                                             CACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCT                                                                     <  SRR19973061.3607507/150‑1 (MQ=60)
                                                                             CACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCT                                                                     <  SRR19973061.1707519/150‑1 (MQ=60)
                                                                             CACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCT                                                                     >  SRR19973061.2390602/1‑150 (MQ=60)
                                                                             CACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCT                                                                     <  SRR19973061.1707520/150‑1 (MQ=60)
                                                                             CACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCT                                                                     <  SRR19973061.711073/150‑1 (MQ=60)
                                                                                   GGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGG                                                               <  SRR19973061.1857619/150‑1 (MQ=60)
                                                                                    GGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGC                                                              <  SRR19973061.2839214/150‑1 (MQ=60)
                                                                                     GCAAGACGATCAGCGCACTTGTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCT                                                             <  SRR19973061.3441232/150‑1 (MQ=60)
                                                                                      CAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTT                                                            >  SRR19973061.2570282/1‑150 (MQ=60)
                                                                                          ACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTC                                                        <  SRR19973061.3414558/150‑1 (MQ=60)
                                                                                            GATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGA                                                      <  SRR19973061.2808852/150‑1 (MQ=60)
                                                                                            GATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGA                                                      <  SRR19973061.2808953/150‑1 (MQ=60)
                                                                                                AGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGAT                                                  >  SRR19973061.1012553/1‑150 (MQ=60)
                                                                                                 GCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATC                                                 <  SRR19973061.2734877/150‑1 (MQ=60)
                                                                                                    CACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATG                                              >  SRR19973061.1157179/1‑150 (MQ=60)
                                                                                                     ACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGA                                             >  SRR19973061.1538164/1‑150 (MQ=60)
                                                                                                       TTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACG                                           <  SRR19973061.3096934/150‑1 (MQ=60)
                                                                                                            TAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGC                                      >  SRR19973061.2957900/1‑150 (MQ=60)
                                                                                                            TAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGC                                      >  SRR19973061.2060189/1‑150 (MQ=60)
                                                                                                            TAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGC                                      >  SRR19973061.3361234/1‑150 (MQ=60)
                                                                                                             AACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCT                                     <  SRR19973061.2941623/150‑1 (MQ=60)
                                                                                                                TGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCC                                  <  SRR19973061.1823847/150‑1 (MQ=60)
                                                                                                                TGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCC                                  <  SRR19973061.1212657/150‑1 (MQ=60)
                                                                                                                TGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCC                                  <  SRR19973061.1823908/150‑1 (MQ=60)
                                                                                                                TGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCC                                  >  SRR19973061.1319739/1‑150 (MQ=60)
                                                                                                                TGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCC                                  >  SRR19973061.1318894/1‑150 (MQ=60)
                                                                                                                  CTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGG                                >  SRR19973061.688414/1‑150 (MQ=60)
                                                                                                                   TGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGG                               >  SRR19973061.3245065/1‑150 (MQ=60)
                                                                                                                       TGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCA                           >  SRR19973061.2579776/1‑150 (MQ=60)
                                                                                                                       TGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCA                           <  SRR19973061.1528017/150‑1 (MQ=60)
                                                                                                                        GTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAG                          <  SRR19973061.120549/150‑1 (MQ=60)
                                                                                                                           TTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTC                       <  SRR19973061.3025869/150‑1 (MQ=60)
                                                                                                                           TTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTC                       <  SRR19973061.3703932/150‑1 (MQ=60)
                                                                                                                            TTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCG                      >  SRR19973061.2181498/1‑150 (MQ=60)
                                                                                                                                 TTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAA                 >  SRR19973061.1616128/1‑150 (MQ=60)
                                                                                                                                    GGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCC              >  SRR19973061.3582280/1‑150 (MQ=60)
                                                                                                                                         GGTAGGCTATGGTGATGTTTTTTGCCAGGTCGAGCTGTTGTCGATTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCT         <  SRR19973061.387042/150‑1 (MQ=60)
                                                                                                                                         GGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCT         >  SRR19973061.688191/1‑150 (MQ=60)
                                                                                                                                            AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT      <  SRR19973061.664202/150‑1 (MQ=60)
                                                                                                                                            AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT      >  SRR19973061.3528374/1‑150 (MQ=60)
                                                                                                                                            AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT      >  SRR19973061.2868142/1‑150 (MQ=60)
                                                                                                                                            AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT      <  SRR19973061.3605283/150‑1 (MQ=60)
                                                                                                                                               CTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAA   <  SRR19973061.3415404/150‑1 (MQ=60)
                                                                                                                                               CTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTACGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCATTTCGCCAAATCCACTCTCTTTAA   <  SRR19973061.3415300/150‑1 (MQ=60)
                                                                                                                                                TATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAAC  <  SRR19973061.275571/150‑1 (MQ=60)
                                                                                                                                                TATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAAC  <  SRR19973061.275689/150‑1 (MQ=60)
                                                                                                                                                TATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAAC  <  SRR19973061.3042325/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
AATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAAC  >  CP009273/1886581‑1886874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑