Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,886,725 G→T 100% I256I (ATC→ATA)  yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,886,7250GT100.0% 763.8 / NA 230I256I (ATC→ATA) yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (108/122);  total (108/122)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

GAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCT  >  CP009273/1886580‑1886869
                                                                                                                                                 |                                                                                                                                                
gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTAt                                                                                                                                                >  1:3713759/1‑148 (MQ=255)
gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTAt                                                                                                                                                >  2:2651372/1‑148 (MQ=255)
  aTTTGCGGTTTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATgg                                                                                                                                              <  1:945604/148‑1 (MQ=255)
   tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGt                                                                                                                                             >  1:1826680/1‑148 (MQ=255)
         gATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTc                                                                                                                                       >  1:2876257/1‑148 (MQ=255)
          aTTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCt                                                                                                                                      >  2:3525982/1‑148 (MQ=255)
           ttAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCtt                                                                                                                                     <  1:2126835/148‑1 (MQ=255)
           ttAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCtt                                                                                                                                     <  1:2222005/148‑1 (MQ=255)
            tAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCttt                                                                                                                                    >  1:2981871/1‑148 (MQ=255)
              gCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTg                                                                                                                                  <  2:3453151/148‑1 (MQ=255)
               cTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGc                                                                                                                                 >  1:1160916/1‑148 (MQ=255)
               cTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGc                                                                                                                                 <  2:3575915/148‑1 (MQ=255)
               cTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGc                                                                                                                                 <  2:1383241/148‑1 (MQ=255)
                  aCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAg                                                                                                                              >  2:1793853/1‑148 (MQ=255)
                  aCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAg                                                                                                                              >  2:1063214/1‑148 (MQ=255)
                    aGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGt                                                                                                                            <  2:2782873/148‑1 (MQ=255)
                      cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCg                                                                                                                          >  1:2734240/1‑148 (MQ=255)
                      cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCg                                                                                                                          >  1:2452993/1‑148 (MQ=255)
                          cGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCa                                                                                                                      >  1:730661/1‑148 (MQ=255)
                          cGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCa                                                                                                                      >  1:1416247/1‑148 (MQ=255)
                           gCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAg                                                                                                                     <  1:1686488/148‑1 (MQ=255)
                           gCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAg                                                                                                                     <  1:1794414/148‑1 (MQ=255)
                                  aCCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGa                                                                                                              <  1:964319/148‑1 (MQ=255)
                                   ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGAc                                                                                                             <  1:831729/148‑1 (MQ=255)
                                   ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGAACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGACCAGTTGTCGAc                                                                                                             <  2:688955/148‑1 (MQ=255)
                                    cgcgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACt                                                                                                            <  1:2769328/148‑1 (MQ=255)
                                      cgATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTgg                                                                                                          >  2:21360/1‑148 (MQ=255)
                                          aaCCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGagag                                                                                                      >  1:231069/1‑148 (MQ=255)
                                              tGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgac                                                                                                  <  1:2909785/148‑1 (MQ=255)
                                              tGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgac                                                                                                  >  2:2873749/1‑148 (MQ=255)
                                                gCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgactg                                                                                                >  1:2506898/1‑148 (MQ=255)
                                                  tCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgactgac                                                                                              >  2:3342402/1‑148 (MQ=255)
                                                   cACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGAcc                                                                                             >  2:967766/1‑148 (MQ=255)
                                                      cGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCaaa                                                                                          <  2:1559330/148‑1 (MQ=255)
                                                       gAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCaaaa                                                                                         <  1:908170/148‑1 (MQ=255)
                                                        aGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAAt                                                                                        >  1:442756/1‑148 (MQ=255)
                                                          ttGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATAc                                                                                      >  2:851011/1‑148 (MQ=255)
                                                              aGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggc                                                                                  <  1:1200765/148‑1 (MQ=255)
                                                              aGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggc                                                                                  <  1:2101961/148‑1 (MQ=255)
                                                              aGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggc                                                                                  <  1:2985702/148‑1 (MQ=255)
                                                               gACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggct                                                                                 >  1:1877251/1‑148 (MQ=255)
                                                                     aaTCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAAt                                                                           >  1:1976886/1‑148 (MQ=255)
                                                                       tCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGt                                                                         >  2:2438304/1‑148 (MQ=255)
                                                                       tCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGt                                                                         <  1:482673/148‑1 (MQ=255)
                                                                         cTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTcc                                                                       <  2:3204480/148‑1 (MQ=255)
                                                                          tGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCg                                                                      >  1:2656482/1‑148 (MQ=255)
                                                                               aCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGc                                                                 <  2:1161800/148‑1 (MQ=255)
                                                                                 tctGGGGAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtg                                                               >  2:2930559/1‑148 (MQ=255)
                                                                                 tctGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtg                                                               >  2:1491001/1‑148 (MQ=255)
                                                                                  ctGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtgg                                                              <  1:90233/148‑1 (MQ=255)
                                                                                    gggCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtggtg                                                            <  2:1259514/148‑1 (MQ=255)
                                                                                     ggCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCtggtgg                                                           >  2:218490/1‑148 (MQ=255)
                                                                                       cAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGc                                                         >  1:2244914/1‑148 (MQ=255)
                                                                                         aGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCtt                                                       >  2:3164407/1‑148 (MQ=255)
                                                                                         aGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCtt                                                       <  1:3162537/148‑1 (MQ=255)
                                                                                               tCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGa                                                 >  2:2634044/1‑148 (MQ=255)
                                                                                                cAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGaa                                                <  1:547832/148‑1 (MQ=255)
                                                                                                  gcgcACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGa                                              <  2:888819/148‑1 (MQ=255)
                                                                                                   cgcACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGAt                                             >  2:702970/1‑148 (MQ=255)
                                                                                                    gcACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATc                                            <  2:3248847/148‑1 (MQ=255)
                                                                                                    gcACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATc                                            <  1:754739/148‑1 (MQ=255)
                                                                                                     cACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCa                                           <  2:2734240/148‑1 (MQ=255)
                                                                                                      aCTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCAt                                          >  2:1737273/1‑148 (MQ=255)
                                                                                                       cTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATg                                         <  1:3027287/148‑1 (MQ=255)
                                                                                                       cTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATg                                         <  1:1639977/148‑1 (MQ=255)
                                                                                                        ttttGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGa                                        >  2:278834/1‑148 (MQ=255)
                                                                                                         tttGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGAc                                       >  2:953524/1‑148 (MQ=255)
                                                                                                              aaCTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCg                                  >  1:1063966/1‑148 (MQ=255)
                                                                                                              aaCTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCg                                  <  2:807113/148‑1 (MQ=255)
                                                                                                                ctgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCt                                >  2:1023880/1‑148 (MQ=255)
                                                                                                                ctgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCt                                >  2:2099728/1‑148 (MQ=255)
                                                                                                                ctgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCt                                >  2:1705096/1‑148 (MQ=255)
                                                                                                                ctgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCt                                >  2:1753165/1‑148 (MQ=255)
                                                                                                                 tgctgGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCtt                               <  2:3723952/148‑1 (MQ=255)
                                                                                                                      ggTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCggg                          >  1:1370811/1‑148 (MQ=255)
                                                                                                                      ggTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCggg                          >  2:3637244/1‑148 (MQ=255)
                                                                                                                        tgtCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGAt                        <  1:2438304/148‑1 (MQ=255)
                                                                                                                           cTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAg                     >  2:1966012/1‑148 (MQ=255)
                                                                                                                            ttttAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGt                    <  1:341469/148‑1 (MQ=255)
                                                                                                                               tAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCg                 <  2:1320359/148‑1 (MQ=255)
                                                                                                                                aaTTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGc                >  1:210806/1‑148 (MQ=255)
                                                                                                                                aaTTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGc                <  1:3538483/148‑1 (MQ=255)
                                                                                                                                aaTTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGc                >  1:655723/1‑148 (MQ=255)
                                                                                                                                 aTTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGcc               >  1:1302214/1‑148 (MQ=255)
                                                                                                                                  ttCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCa              >  2:45092/1‑148 (MQ=255)
                                                                                                                                  ttCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCa              >  2:2539813/1‑148 (MQ=255)
                                                                                                                                    cGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCaaa            <  1:103497/148‑1 (MQ=255)
                                                                                                                                     ggTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAAt           <  2:730661/148‑1 (MQ=255)
                                                                                                                                     ggTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAAt           <  2:1416247/148‑1 (MQ=255)
                                                                                                                                      gTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATc          <  2:2328766/148‑1 (MQ=255)
                                                                                                                                      gTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATc          <  1:2554857/148‑1 (MQ=255)
                                                                                                                                      gTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATc          <  1:3342402/148‑1 (MQ=255)
                                                                                                                                        gCGGTAGGCTATGGTGATGTCTTTTGCCATGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCa        <  2:3531792/148‑1 (MQ=255)
                                                                                                                                            tAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActct    >  2:230768/1‑148 (MQ=255)
                                                                                                                                             aGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActctc   <  2:3087711/148‑1 (MQ=255)
                                                                                                                                             aGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActctc   <  2:2506898/148‑1 (MQ=255)
                                                                                                                                             aGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActctc   <  2:597025/148‑1 (MQ=255)
                                                                                                                                             aGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActctc   <  1:3178447/148‑1 (MQ=255)
                                                                                                                                              ggCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActctct  >  1:1587183/1‑148 (MQ=255)
                                                                                                                                              ggCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCActctct  <  2:1458712/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCT  >  CP009273/1886580‑1886869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAA  >  CP009273/1886576‑1886873
                                                                                                                                                     |                                                                                                                                                    
CGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCT                                                                                                                                                      >  SRR19973069.2664935/1‑150 (MQ=60)
CGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCT                                                                                                                                                      >  SRR19973069.2664932/1‑150 (MQ=60)
CGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCT                                                                                                                                                      >  SRR19973069.1562992/1‑150 (MQ=60)
  CTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTAT                                                                                                                                                    >  SRR19973069.3738012/1‑150 (MQ=60)
   TGACTGTGCGGATTAGCTAACCGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATG                                                                                                                                                   <  SRR19973069.2608651/150‑1 (MQ=60)
   TGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATG                                                                                                                                                   <  SRR19973069.3766637/150‑1 (MQ=60)
   TGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATG                                                                                                                                                   <  SRR19973069.2358415/150‑1 (MQ=60)
   TGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATG                                                                                                                                                   <  SRR19973069.1597324/150‑1 (MQ=60)
     AATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGT                                                                                                                                                 >  SRR19973069.998577/1‑150 (MQ=60)
     AATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGT                                                                                                                                                 >  SRR19973069.1839490/1‑150 (MQ=60)
      ATTTGCGGTTTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTG                                                                                                                                                <  SRR19973069.951975/150‑1 (MQ=60)
      ATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTG                                                                                                                                                <  SRR19973069.951935/150‑1 (MQ=60)
           CGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTC                                                                                                                                           >  SRR19973069.2895128/1‑150 (MQ=60)
           CGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTC                                                                                                                                           >  SRR19973069.3769549/1‑150 (MQ=60)
              ATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTT                                                                                                                                        >  SRR19973069.3001670/1‑150 (MQ=60)
              ATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTT                                                                                                                                        >  SRR19973069.1081114/1‑150 (MQ=60)
               TTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTT                                                                                                                                       <  SRR19973069.2141470/150‑1 (MQ=60)
               TTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTT                                                                                                                                       <  SRR19973069.2141655/150‑1 (MQ=60)
               TTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTT                                                                                                                                       <  SRR19973069.2237034/150‑1 (MQ=60)
               TTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTT                                                                                                                                       <  SRR19973069.2375660/150‑1 (MQ=60)
                 AGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGC                                                                                                                                     >  SRR19973069.1168989/1‑150 (MQ=60)
                 AGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGC                                                                                                                                     >  SRR19973069.1169878/1‑150 (MQ=60)
                    TAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAG                                                                                                                                  >  SRR19973069.3270116/1‑150 (MQ=60)
                        AGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCG                                                                                                                              >  SRR19973069.2752926/1‑150 (MQ=60)
                        AGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCG                                                                                                                              >  SRR19973069.2469469/1‑150 (MQ=60)
                        AGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCG                                                                                                                              >  SRR19973069.2469490/1‑150 (MQ=60)
                        AGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCG                                                                                                                              >  SRR19973069.2752538/1‑150 (MQ=60)
                            CACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCA                                                                                                                          >  SRR19973069.2664236/1‑150 (MQ=60)
                            CACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCA                                                                                                                          >  SRR19973069.1426127/1‑150 (MQ=60)
                            CACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTAGGGTGATGTCTTTTGCCAGGTCGAGCA                                                                                                                          >  SRR19973069.735827/1‑150 (MQ=60)
                               GCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTT                                                                                                                       <  SRR19973069.1807050/150‑1 (MQ=60)
                               GCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTT                                                                                                                       <  SRR19973069.1698260/150‑1 (MQ=60)
                               GCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTT                                                                                                                       <  SRR19973069.1695022/150‑1 (MQ=60)
                                CAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTG                                                                                                                      <  SRR19973069.2535794/150‑1 (MQ=60)
                                 AGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGT                                                                                                                     >  SRR19973069.2180462/1‑150 (MQ=60)
                                     TACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGAC                                                                                                                 <  SRR19973069.2119635/150‑1 (MQ=60)
                                      ACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACT                                                                                                                <  SRR19973069.970754/150‑1 (MQ=60)
                                       CCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTG                                                                                                               <  SRR19973069.837575/150‑1 (MQ=60)
                                       CCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTG                                                                                                               <  SRR19973069.837580/150‑1 (MQ=60)
                                        CGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGG                                                                                                              <  SRR19973069.2787785/150‑1 (MQ=60)
                                        CGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGG                                                                                                              <  SRR19973069.2787894/150‑1 (MQ=60)
                                        CGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGG                                                                                                              <  SRR19973069.1392691/150‑1 (MQ=60)
                                            ATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAG                                                                                                          >  SRR19973069.232836/1‑150 (MQ=60)
                                             TAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGT                                                                                                         <  SRR19973069.739845/150‑1 (MQ=60)
                                                CCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGAC                                                                                                      <  SRR19973069.37486/150‑1 (MQ=60)
                                                  TGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTG                                                                                                    <  SRR19973069.1644568/150‑1 (MQ=60)
                                                  TGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTG                                                                                                    >  SRR19973069.2523615/1‑150 (MQ=60)
                                                  TGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTG                                                                                                    <  SRR19973069.2928640/150‑1 (MQ=60)
                                                  TGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTG                                                                                                    <  SRR19973069.2929035/150‑1 (MQ=60)
                                                         CCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAA                                                                                             >  SRR19973069.812831/1‑150 (MQ=60)
                                                          CGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAAT                                                                                            >  SRR19973069.2286413/1‑150 (MQ=60)
                                                          CGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAAT                                                                                            >  SRR19973069.445992/1‑150 (MQ=60)
                                                           GAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATA                                                                                           <  SRR19973069.914393/150‑1 (MQ=60)
                                                           GAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAANA                                                                                           <  SRR19973069.2839997/150‑1 (MQ=60)
                                                            AGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATAC                                                                                          >  SRR19973069.676962/1‑150 (MQ=60)
                                                             GTTGCAGACGAAACTCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACT                                                                                         <  SRR19973069.1229545/150‑1 (MQ=60)
                                                               TGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGG                                                                                       <  SRR19973069.2974282/150‑1 (MQ=60)
                                                                 CAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCT                                                                                     >  SRR19973069.1890303/1‑150 (MQ=60)
                                                                 CAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCT                                                                                     >  SRR19973069.1842849/1‑150 (MQ=60)
                                                                  AGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTG                                                                                    <  SRR19973069.3005501/150‑1 (MQ=60)
                                                                  AGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTG                                                                                    <  SRR19973069.3005532/150‑1 (MQ=60)
                                                                  AGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTG                                                                                    <  SRR19973069.1289701/150‑1 (MQ=60)
                                                                  AGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTG                                                                                    <  SRR19973069.3005530/150‑1 (MQ=60)
                                                                  AGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTG                                                                                    <  SRR19973069.1209023/150‑1 (MQ=60)
                                                                  AGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTG                                                                                    <  SRR19973069.2116471/150‑1 (MQ=60)
                                                                       AAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAAT                                                                               >  SRR19973069.1990636/1‑150 (MQ=60)
                                                                       AAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAAT                                                                               <  SRR19973069.2074512/150‑1 (MQ=60)
                                                                           TCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCTACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCC                                                                           <  SRR19973069.1429110/150‑1 (MQ=60)
                                                                           TCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCC                                                                           <  SRR19973069.486174/150‑1 (MQ=60)
                                                                            CCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCG                                                                          >  SRR19973069.2674288/1‑150 (MQ=60)
                                                                               GCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTA                                                                       <  SRR19973069.1814966/150‑1 (MQ=60)
                                                                                   ACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTG                                                                   >  SRR19973069.3580240/1‑150 (MQ=60)
                                                                                     TCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGT                                                                 <  SRR19973069.3371288/150‑1 (MQ=60)
                                                                                      CTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTG                                                                <  SRR19973069.91169/150‑1 (MQ=60)
                                                                                         GGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGC                                                             >  SRR19973069.2480438/1‑150 (MQ=60)
                                                                                         GGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGC                                                             >  SRR19973069.2259830/1‑150 (MQ=60)
                                                                                         GGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGC                                                             >  SRR19973069.2260031/1‑150 (MQ=60)
                                                                                             AGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCG                                                         <  SRR19973069.3183166/150‑1 (MQ=60)
                                                                                                  ATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAA                                                    >  SRR19973069.957738/1‑150 (MQ=60)
                                                                                                  ATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAA                                                    >  SRR19973069.3202131/1‑150 (MQ=60)
                                                                                                   TCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAG                                                   <  SRR19973069.3508883/150‑1 (MQ=60)
                                                                                                    CAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGA                                                  <  SRR19973069.551671/150‑1 (MQ=60)
                                                                                                     AGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGAT                                                 >  SRR19973069.1562800/1‑150 (MQ=60)
                                                                                                     AGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGAT                                                 >  SRR19973069.2660453/1‑150 (MQ=60)
                                                                                                     AGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGAT                                                 <  SRR19973069.92985/150‑1 (MQ=60)
                                                                                                        GCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCAT                                              <  SRR19973069.760077/150‑1 (MQ=60)
                                                                                                           CTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTGCGGTAGCTGGTGGGCTTCGTCGAAGATCATGAC                                           <  SRR19973069.3047357/150‑1 (MQ=60)
                                                                                                           CTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGAC                                           <  SRR19973069.3047368/150‑1 (MQ=60)
                                                                                                           CTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGAC                                           >  SRR19973069.1903731/1‑150 (MQ=60)
                                                                                                           CTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGAC                                           <  SRR19973069.1651238/150‑1 (MQ=60)
                                                                                                            TTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACT                                          <  SRR19973069.3176750/150‑1 (MQ=60)
                                                                                                            TTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACG                                          <  SRR19973069.909846/150‑1 (MQ=60)
                                                                                                             TTTGTAACTGCTGGGTGTCTTTTAAGTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGT                                         >  SRR19973069.601061/1‑150 (MQ=60)
                                                                                                                GTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCG                                      >  SRR19973069.1071377/1‑150 (MQ=60)
                                                                                                                        TGGGTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGG                              >  SRR19973069.1380346/1‑150 (MQ=60)
                                                                                                                           GTGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATC                           <  SRR19973069.2375928/150‑1 (MQ=60)
                                                                                                                            TGTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCA                          <  SRR19973069.2454738/150‑1 (MQ=60)
                                                                                                                             GTCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAG                         <  SRR19973069.1070621/150‑1 (MQ=60)
                                                                                                                              TCTTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGT                        >  SRR19973069.2714997/1‑150 (MQ=60)
                                                                                                                                TTTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTC                      <  SRR19973069.343871/150‑1 (MQ=60)
                                                                                                                                 TTTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCG                     <  SRR19973069.2125932/150‑1 (MQ=60)
                                                                                                                                  TTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGC                    >  SRR19973069.212472/1‑150 (MQ=60)
                                                                                                                                  TTAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGC                    >  SRR19973069.660126/1‑150 (MQ=60)
                                                                                                                                   TAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCC                   >  SRR19973069.1311659/1‑150 (MQ=60)
                                                                                                                                   TAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCC                   >  SRR19973069.1311036/1‑150 (MQ=60)
                                                                                                                                   TAATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCC                   >  SRR19973069.545890/1‑150 (MQ=60)
                                                                                                                                    AATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCA                  <  SRR19973069.3561435/150‑1 (MQ=60)
                                                                                                                                    AATTCGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCA                  <  SRR19973069.549870/150‑1 (MQ=60)
                                                                                                                                        CGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATC              <  SRR19973069.104526/150‑1 (MQ=60)
                                                                                                                                        CGGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATC              <  SRR19973069.2048127/150‑1 (MQ=60)
                                                                                                                                         GGTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCC             <  SRR19973069.892660/150‑1 (MQ=60)
                                                                                                                                          GTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCA            <  SRR19973069.2571731/150‑1 (MQ=60)
                                                                                                                                          GTGCGGTAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCA            <  SRR19973069.3364284/150‑1 (MQ=60)
                                                                                                                                                TAGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCT      >  SRR19973069.1597932/1‑150 (MQ=60)
                                                                                                                                                 AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT     <  SRR19973069.3196517/150‑1 (MQ=60)
                                                                                                                                                 AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT     <  SRR19973069.3199122/150‑1 (MQ=60)
                                                                                                                                                 AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT     <  SRR19973069.1749460/150‑1 (MQ=60)
                                                                                                                                                 AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT     <  SRR19973069.1749385/150‑1 (MQ=60)
                                                                                                                                                 AGGCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTT     <  SRR19973069.468873/150‑1 (MQ=60)
                                                                                                                                                   GCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTA   >  SRR19973069.3587749/1‑150 (MQ=60)
                                                                                                                                                   GCTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTA   >  SRR19973069.3589480/1‑150 (MQ=60)
                                                                                                                                                    CTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAA  >  SRR19973069.2597751/1‑150 (MQ=60)
                                                                                                                                                    CTATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAA  >  SRR19973069.349078/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGGCAATGTCCGGTAGCTGGTGGGCTTCGTCGAAGATCATGACGTCCGCTTCCGGGATCAGTTCGCCAAATCCACTCTCTTTAA  >  CP009273/1886576‑1886873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑