Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 208,013 G→T 100% M616I (ATG→ATT)  ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273208,0130GT98.9% 896.3 / 0.2 274M616I (ATG→ATT) ldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (124/147);  total (125/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 282 total aligned reads displayed.

AAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG  >  CP009273/207866‑208160
                                                                                                                                                   |                                                                                                                                                   
aaaTATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAgattat                                                                                                                                                   >  2:2815914/1‑150 (MQ=255)
aaaTATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAgattat                                                                                                                                                   >  2:4253054/1‑150 (MQ=255)
    atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATg                                                                                                                                               <  2:1871015/150‑1 (MQ=255)
    atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATg                                                                                                                                               <  2:4568818/150‑1 (MQ=255)
     tGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGa                                                                                                                                              <  2:4716547/150‑1 (MQ=255)
     tGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGa                                                                                                                                              >  2:4998710/1‑150 (MQ=255)
       cTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACg                                                                                                                                            <  1:4172847/150‑1 (MQ=255)
         aCCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGcc                                                                                                                                          >  2:1682105/1‑150 (MQ=255)
          cccGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGNGCATTCGATACTTTGCCGGAGATTATCATGACGCca                                                                                                                                         <  1:2677344/150‑1 (MQ=255)
          cccGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCca                                                                                                                                         <  1:2335932/150‑1 (MQ=255)
            cGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCcaca                                                                                                                                       >  1:136631/1‑150 (MQ=255)
            cGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCcaca                                                                                                                                       <  1:783526/150‑1 (MQ=255)
              aTCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATc                                                                                                                                     >  2:520930/1‑150 (MQ=255)
              aTCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATc                                                                                                                                     <  1:3962351/150‑1 (MQ=255)
                 tctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAgg                                                                                                                                  <  1:2057345/150‑1 (MQ=255)
                  ctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGc                                                                                                                                 <  2:71935/150‑1 (MQ=255)
                     tGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATTAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGTCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATg                                                                                                                              <  1:3672659/150‑1 (MQ=255)
                      gCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATgg                                                                                                                             >  1:4594085/1‑150 (MQ=255)
                      gCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATgg                                                                                                                             <  2:677674/150‑1 (MQ=255)
                      gCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCCGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATgg                                                                                                                             <  2:752178/150‑1 (MQ=255)
                       cAGACGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGc                                                                                                                            <  1:4035389/150‑1 (MQ=255)
                        agaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCa                                                                                                                           <  2:1155432/150‑1 (MQ=255)
                         gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCaa                                                                                                                          <  1:3447879/150‑1 (MQ=255)
                         gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCaa                                                                                                                          <  1:975257/150‑1 (MQ=255)
                           agaTCCCGATTTCTACCGCAATATGCGTATTCAGGCTCTGGCACAAGGGATCCATAAGCTGATTCGTAACCACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACg                                                                                                                        <  2:3099018/150‑1 (MQ=255)
                           agaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACg                                                                                                                        >  1:717976/1‑150 (MQ=255)
                            gaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGa                                                                                                                       >  2:2008672/1‑150 (MQ=255)
                              tCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACa                                                                                                                     <  2:1408661/150‑1 (MQ=255)
                                ccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACaaa                                                                                                                   >  1:4931895/1‑150 (MQ=255)
                                ccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACaaa                                                                                                                   >  1:1258047/1‑150 (MQ=255)
                                  gATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAAtt                                                                                                                 >  1:1763854/1‑150 (MQ=255)
                                   aTTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTa                                                                                                                <  1:417990/150‑1 (MQ=255)
                                     ttCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTaaa                                                                                                              >  2:2408356/1‑150 (MQ=255)
                                       cTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAgg                                                                                                            >  2:441354/1‑150 (MQ=255)
                                         aCCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACACATTAAAGGCg                                                                                                          >  2:2978156/1‑150 (MQ=255)
                                          ccGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGa                                                                                                         >  1:1111835/1‑150 (MQ=255)
                                            gCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTACACACGATCTTCCCGGGTTGATGTTGCGGGCATTCGATACTTTGCCGGCGATTATCATGACGCCACATCAGGCATGGCACCGACAACTTAAAGGCgccg                                                                                                       >  2:3341676/1‑147 (MQ=255)
                                                 atGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGaa                                                                                                  <  2:1820268/150‑1 (MQ=255)
                                                  tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGaaa                                                                                                 >  2:2865048/1‑150 (MQ=255)
                                                  tGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGaaa                                                                                                 >  1:3208067/1‑150 (MQ=255)
                                                       aTTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAtt                                                                                            >  1:1608330/1‑150 (MQ=255)
                                                       aTTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAtt                                                                                            >  1:364452/1‑150 (MQ=255)
                                                        ttCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTg                                                                                           >  1:3036889/1‑150 (MQ=255)
                                                        ttCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTg                                                                                           <  1:2980607/150‑1 (MQ=255)
                                                        ttCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTg                                                                                           <  1:2477940/150‑1 (MQ=255)
                                                          cAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgcg                                                                                         <  2:278590/150‑1 (MQ=255)
                                                           aGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgcgc                                                                                        >  1:58674/1‑150 (MQ=255)
                                                           aGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACTCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgcgc                                                                                        >  2:3548309/1‑150 (MQ=255)
                                                            ggATCTGGCACTAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGctct                                                                                       <  1:2315890/150‑1 (MQ=255)
                                                             gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTg                                                                                      >  1:1014825/1‑150 (MQ=255)
                                                               tCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGa                                                                                    <  1:1557617/150‑1 (MQ=255)
                                                                 tGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGaac                                                                                  <  2:953000/150‑1 (MQ=255)
                                                                       aaGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTgg                                                                            >  2:3509941/1‑150 (MQ=255)
                                                                       aaGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTgg                                                                            >  2:624714/1‑150 (MQ=255)
                                                                       aaGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTgg                                                                            >  1:3171633/1‑150 (MQ=255)
                                                                        aGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGt                                                                           >  1:989589/1‑150 (MQ=255)
                                                                         gggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTc                                                                          >  2:3960944/1‑150 (MQ=255)
                                                                          ggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCg                                                                         >  2:786171/1‑150 (MQ=255)
                                                                          ggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCg                                                                         >  2:4349617/1‑150 (MQ=255)
                                                                            aTCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGt                                                                       <  2:4162488/150‑1 (MQ=255)
                                                                             tCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTa                                                                      >  2:4769092/1‑150 (MQ=255)
                                                                             tCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTa                                                                      <  1:5030248/150‑1 (MQ=255)
                                                                              ccATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACGACTGGTCGGTag                                                                     >  2:1915371/1‑150 (MQ=255)
                                                                              ccATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTag                                                                     >  2:4353105/1‑150 (MQ=255)
                                                                                     cTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCg                                                              >  2:3747448/1‑150 (MQ=255)
                                                                                     cTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCg                                                              <  1:4763669/150‑1 (MQ=255)
                                                                                       gATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGc                                                            <  2:1491545/150‑1 (MQ=255)
                                                                                             tAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAatat                                                      >  2:2548685/1‑150 (MQ=255)
                                                                                             tAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAatat                                                      >  2:2736886/1‑150 (MQ=255)
                                                                                              aaaCACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATg                                                     <  2:236913/150‑1 (MQ=255)
                                                                                                 cacGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTGTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATc                                                  <  2:3201046/150‑1 (MQ=255)
                                                                                                   cGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCt                                                >  1:4449787/1‑150 (MQ=255)
                                                                                                   cGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCt                                                >  2:3043090/1‑150 (MQ=255)
                                                                                                   cGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCt                                                <  1:3073120/150‑1 (MQ=255)
                                                                                                    gATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTg                                               <  2:4874168/150‑1 (MQ=255)
                                                                                                    gATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTg                                               <  2:34286/150‑1 (MQ=255)
                                                                                                         tCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTa                                          >  2:1497459/1‑150 (MQ=255)
                                                                                                          cccGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAt                                         >  1:328709/1‑150 (MQ=255)
                                                                                                          cccGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAt                                         >  1:4302860/1‑150 (MQ=255)
                                                                                                           ccGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATc                                        >  1:4708733/1‑150 (MQ=255)
                                                                                                              gTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCAc                                     <  2:1576594/150‑1 (MQ=255)
                                                                                                               tttGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCAcc                                    <  2:1258047/150‑1 (MQ=255)
                                                                                                                ttGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCg                                   >  1:5102335/1‑150 (MQ=255)
                                                                                                                     gTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGt                              <  1:5053666/150‑1 (MQ=255)
                                                                                                                         cGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTAcca                          <  2:2053162/150‑2 (MQ=255)
                                                                                                                         cGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCg                          <  2:3184259/150‑1 (MQ=255)
                                                                                                                                tCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGa                   >  1:861768/1‑150 (MQ=255)
                                                                                                                                tCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGa                   <  2:2191010/150‑1 (MQ=255)
                                                                                                                                   aTACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc                <  1:4769092/150‑1 (MQ=255)
                                                                                                                                   aTACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc                <  1:222229/150‑1 (MQ=255)
                                                                                                                                    tACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGcc               <  1:67016/150‑1 (MQ=255)
                                                                                                                                    tACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGAAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGAGGcc               >  2:3685427/1‑150 (MQ=255)
                                                                                                                                      cTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTg             <  1:4253054/150‑1 (MQ=255)
                                                                                                                                      cTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTg             <  1:1675712/150‑1 (MQ=255)
                                                                                                                                           ccGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGaa        >  1:1130913/1‑150 (MQ=255)
                                                                                                                                           ccGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGaa        <  2:4928408/150‑1 (MQ=255)
                                                                                                                                            cGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGaaa       >  1:4551612/1‑150 (MQ=255)
                                                                                                                                              gagaTTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATg     <  2:3617893/150‑1 (MQ=255)
                                                                                                                                               agaTTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGc    <  2:1763854/150‑1 (MQ=255)
                                                                                                                                                gattatCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCt   <  2:2293788/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG  >  CP009273/207866‑208160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTGA  >  CP009273/207866‑208161
                                                                                                                                                   |                                                                                                                                                    
AAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTAT                                                                                                                                                    >  SRR19973063.4574949/1‑150 (MQ=60)
  ATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCA                                                                                                                                                  <  SRR19973063.3731969/150‑1 (MQ=60)
   TATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCAT                                                                                                                                                 >  SRR19973063.1825929/1‑150 (MQ=60)
       CTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACG                                                                                                                                             <  SRR19973063.4186180/150‑1 (MQ=60)
          CCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGNGCATTCGATACTTTGCCGGAGATTATCATGACGCCA                                                                                                                                          <  SRR19973063.2685951/150‑1 (MQ=60)
          CCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCA                                                                                                                                          <  SRR19973063.2343402/150‑1 (MQ=60)
           CCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCAC                                                                                                                                         >  SRR19973063.102043/1‑150 (MQ=60)
           CCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCAC                                                                                                                                         <  SRR19973063.3375862/150‑1 (MQ=60)
            CGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACA                                                                                                                                        <  SRR19973063.785795/150‑1 (MQ=60)
            CGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACA                                                                                                                                        >  SRR19973063.137062/1‑150 (MQ=60)
              ATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATC                                                                                                                                      <  SRR19973063.3975032/150‑1 (MQ=60)
                 TCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGG                                                                                                                                   <  SRR19973063.2063773/150‑1 (MQ=60)
                  CTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGC                                                                                                                                  <  SRR19973063.5067476/150‑1 (MQ=60)
                     TGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATTAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGTCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATG                                                                                                                               <  SRR19973063.3684424/150‑1 (MQ=60)
                     TGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATG                                                                                                                               >  SRR19973063.1127195/1‑150 (MQ=60)
                      GCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGG                                                                                                                              >  SRR19973063.4608785/1‑150 (MQ=60)
                       CAGACGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGC                                                                                                                             <  SRR19973063.4048319/150‑1 (MQ=60)
                       CAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGC                                                                                                                             >  SRR19973063.5152552/1‑150 (MQ=60)
                        AGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCA                                                                                                                            >  SRR19973063.450146/1‑150 (MQ=60)
                         GAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAA                                                                                                                           <  SRR19973063.3458837/150‑1 (MQ=60)
                         GAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAA                                                                                                                           <  SRR19973063.2556771/150‑1 (MQ=60)
                         GAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAA                                                                                                                           <  SRR19973063.978200/150‑1 (MQ=60)
                         GAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAA                                                                                                                           <  SRR19973063.978224/150‑1 (MQ=60)
                           TGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                         >  SRR19973063.2572319/1‑150 (MQ=60)
                           AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                         >  SRR19973063.720089/1‑150 (MQ=60)
                           AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                         <  SRR19973063.716559/150‑1 (MQ=60)
                           AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                         <  SRR19973063.2373263/150‑1 (MQ=60)
                           AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                         <  SRR19973063.2373202/150‑1 (MQ=60)
                                CCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAA                                                                                                                    >  SRR19973063.4947826/1‑150 (MQ=60)
                                CCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAA                                                                                                                    >  SRR19973063.1261787/1‑150 (MQ=60)
                                CCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGAAAACACGATCTTCACGGTTTGATGTTGCGGGCATTCGATACTTTGCAGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAA                                                                                                                    >  SRR19973063.1856798/1‑150 (MQ=60)
                                 CGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAAT                                                                                                                   <  SRR19973063.1550809/150‑1 (MQ=60)
                                 CGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAAT                                                                                                                   <  SRR19973063.4202137/150‑1 (MQ=60)
                                  GATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATT                                                                                                                  >  SRR19973063.1769282/1‑150 (MQ=60)
                                  GATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATT                                                                                                                  >  SRR19973063.3194495/1‑150 (MQ=60)
                                   ATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTA                                                                                                                 <  SRR19973063.3016421/150‑1 (MQ=60)
                                   ATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTA                                                                                                                 <  SRR19973063.419161/150‑1 (MQ=60)
                                       CTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGG                                                                                                             <  SRR19973063.4186316/150‑1 (MQ=60)
                                       CTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGG                                                                                                             <  SRR19973063.4419477/150‑1 (MQ=60)
                                       CTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGG                                                                                                             >  SRR19973063.4780587/1‑150 (MQ=60)
                                        TACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGC                                                                                                            <  SRR19973063.2136715/150‑1 (MQ=60)
                                          CCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGA                                                                                                          >  SRR19973063.1115216/1‑150 (MQ=60)
                                          CCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGA                                                                                                          <  SRR19973063.1652379/150‑1 (MQ=60)
                                            GCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAG                                                                                                        <  SRR19973063.716256/150‑1 (MQ=60)
                                               ATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAG                                                                                                     <  SRR19973063.119391/150‑1 (MQ=60)
                                                 ATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAA                                                                                                   >  SRR19973063.5048032/1‑150 (MQ=60)
                                                  TGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAA                                                                                                  <  SRR19973063.4009206/150‑1 (MQ=60)
                                                  TGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAA                                                                                                  >  SRR19973063.3218382/1‑150 (MQ=60)
                                                    CGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACC                                                                                                >  SRR19973063.4646067/1‑150 (MQ=60)
                                                     GTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCA                                                                                               >  SRR19973063.4893482/1‑150 (MQ=60)
                                                      TATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAT                                                                                              <  SRR19973063.5145140/150‑1 (MQ=60)
                                                       ATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATT                                                                                             >  SRR19973063.1613190/1‑150 (MQ=60)
                                                       ATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATT                                                                                             >  SRR19973063.1613253/1‑150 (MQ=60)
                                                       ATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATT                                                                                             >  SRR19973063.365497/1‑150 (MQ=60)
                                                        TTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTG                                                                                            >  SRR19973063.3046403/1‑150 (MQ=60)
                                                        TTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTG                                                                                            <  SRR19973063.2990128/150‑1 (MQ=60)
                                                        TTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTG                                                                                            >  SRR19973063.3046595/1‑150 (MQ=60)
                                                        TTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTG                                                                                            <  SRR19973063.2485864/150‑1 (MQ=60)
                                                         TCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGC                                                                                           >  SRR19973063.3205875/1‑150 (MQ=60)
                                                         TCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGC                                                                                           >  SRR19973063.4604153/1‑150 (MQ=60)
                                                           AGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGC                                                                                         >  SRR19973063.58881/1‑150 (MQ=60)
                                                            GGATCTGGCACTAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCTCT                                                                                        <  SRR19973063.2323296/150‑1 (MQ=60)
                                                            GGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCT                                                                                        >  SRR19973063.3136154/1‑150 (MQ=60)
                                                             GATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTG                                                                                       >  SRR19973063.1017883/1‑150 (MQ=60)
                                                               TCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGA                                                                                     <  SRR19973063.1562389/150‑1 (MQ=60)
                                                                CTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCACCGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAA                                                                                    >  SRR19973063.4944327/1‑150 (MQ=60)
                                                                    CACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAAC                                                                                >  SRR19973063.4968676/1‑150 (MQ=60)
                                                                    CACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAAC                                                                                >  SRR19973063.761832/1‑150 (MQ=60)
                                                                       AAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGG                                                                             >  SRR19973063.3181843/1‑150 (MQ=60)
                                                                       AAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGG                                                                             >  SRR19973063.3094395/1‑150 (MQ=60)
                                                                        AGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGT                                                                            >  SRR19973063.992370/1‑150 (MQ=60)
                                                                        AGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGT                                                                            >  SRR19973063.992574/1‑150 (MQ=60)
                                                                          GGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCG                                                                          <  SRR19973063.1315417/150‑1 (MQ=60)
                                                                             TCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTA                                                                       <  SRR19973063.5046298/150‑1 (MQ=60)
                                                                             TCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTA                                                                       <  SRR19973063.5046469/150‑1 (MQ=60)
                                                                                  AAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTA                                                                  <  SRR19973063.450917/150‑1 (MQ=60)
                                                                                  AAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTA                                                                  >  SRR19973063.3520144/1‑150 (MQ=60)
                                                                                  AAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTA                                                                  <  SRR19973063.232547/150‑1 (MQ=60)
                                                                                     CTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCG                                                               <  SRR19973063.4778831/150‑1 (MQ=60)
                                                                                     CTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCG                                                               <  SRR19973063.4778865/150‑1 (MQ=60)
                                                                                        ATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCA                                                            <  SRR19973063.1718250/150‑1 (MQ=60)
                                                                                                ACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGAT                                                    <  SRR19973063.3656557/150‑1 (MQ=60)
                                                                                                 CACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATC                                                   <  SRR19973063.655665/150‑1 (MQ=60)
                                                                                                  ACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCC                                                  <  SRR19973063.4149544/150‑1 (MQ=60)
                                                                                                  ACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCC                                                  <  SRR19973063.2824965/150‑1 (MQ=60)
                                                                                                   CGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCT                                                 <  SRR19973063.3082991/150‑1 (MQ=60)
                                                                                                   CGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCT                                                 >  SRR19973063.4464059/1‑150 (MQ=60)
                                                                                                    GATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTG                                                <  SRR19973063.796624/150‑1 (MQ=60)
                                                                                                    GATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACTCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTG                                                <  SRR19973063.3559679/150‑1 (MQ=60)
                                                                                                      TCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCC                                              >  SRR19973063.4192711/1‑150 (MQ=60)
                                                                                                      TCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCC                                              <  SRR19973063.4280153/150‑1 (MQ=60)
                                                                                                      TCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCC                                              <  SRR19973063.2830874/150‑1 (MQ=60)
                                                                                                        TTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTT                                            <  SRR19973063.1244461/150‑1 (MQ=60)
                                                                                                          CCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAT                                          >  SRR19973063.4316532/1‑150 (MQ=60)
                                                                                                          CCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAT                                          >  SRR19973063.329596/1‑150 (MQ=60)
                                                                                                          CCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAT                                          >  SRR19973063.329664/1‑150 (MQ=60)
                                                                                                           CCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATC                                         >  SRR19973063.1450729/1‑150 (MQ=60)
                                                                                                           CCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATC                                         >  SRR19973063.4723735/1‑150 (MQ=60)
                                                                                                            CGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCC                                        >  SRR19973063.111758/1‑150 (MQ=60)
                                                                                                             GGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCA                                       <  SRR19973063.775522/150‑1 (MQ=60)
                                                                                                              GTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCAC                                      <  SRR19973063.994532/150‑1 (MQ=60)
                                                                                                                TTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCG                                    >  SRR19973063.5118755/1‑150 (MQ=60)
                                                                                                                 TGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGG                                   <  SRR19973063.590073/150‑1 (MQ=60)
                                                                                                                     GTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGT                               >  SRR19973063.3338354/1‑150 (MQ=60)
                                                                                                                     GTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGT                               >  SRR19973063.4279913/1‑150 (MQ=60)
                                                                                                                     GTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGT                               <  SRR19973063.3898542/150‑1 (MQ=60)
                                                                                                                     GTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGT                               <  SRR19973063.5069937/150‑1 (MQ=60)
                                                                                                                         CGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCG                           <  SRR19973063.5100349/150‑1 (MQ=60)
                                                                                                                             CATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGT                       >  SRR19973063.4622694/1‑150 (MQ=60)
                                                                                                                              ATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTT                      <  SRR19973063.3260802/150‑1 (MQ=60)
                                                                                                                              ATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTT                      <  SRR19973063.207469/150‑1 (MQ=60)
                                                                                                                                TCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGA                    >  SRR19973063.2317858/1‑150 (MQ=60)
                                                                                                                                TCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGA                    >  SRR19973063.2320283/1‑150 (MQ=60)
                                                                                                                                TCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGA                    >  SRR19973063.864251/1‑150 (MQ=60)
                                                                                                                                 CGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGAT                   <  SRR19973063.810856/150‑1 (MQ=60)
                                                                                                                                   ATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGC                 <  SRR19973063.222892/150‑1 (MQ=60)
                                                                                                                                   ATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGC                 <  SRR19973063.4784311/150‑1 (MQ=60)
                                                                                                                                    TACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCC                <  SRR19973063.946719/150‑1 (MQ=60)
                                                                                                                                    TACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCC                <  SRR19973063.67250/150‑1 (MQ=60)
                                                                                                                                      CTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTG              <  SRR19973063.4266599/150‑1 (MQ=60)
                                                                                                                                      CTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTG              <  SRR19973063.1680818/150‑1 (MQ=60)
                                                                                                                                       TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGG             >  SRR19973063.4097317/1‑150 (MQ=60)
                                                                                                                                       TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGG             <  SRR19973063.3056751/150‑1 (MQ=60)
                                                                                                                                          GCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGA          >  SRR19973063.1628222/1‑150 (MQ=60)
                                                                                                                                           CCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAA         >  SRR19973063.1134343/1‑150 (MQ=60)
                                                                                                                                            CGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAA        <  SRR19973063.1333286/150‑1 (MQ=60)
                                                                                                                                            CGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAA        >  SRR19973063.4566193/1‑150 (MQ=60)
                                                                                                                                             GGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAAT       <  SRR19973063.1687229/150‑1 (MQ=60)
                                                                                                                                              GAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATG      <  SRR19973063.4011949/150‑1 (MQ=60)
                                                                                                                                                GATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCT    >  SRR19973063.4783114/1‑150 (MQ=60)
                                                                                                                                                 ATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG   <  SRR19973063.2556810/150‑1 (MQ=60)
                                                                                                                                                 ATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG   <  SRR19973063.1258847/150‑1 (MQ=60)
                                                                                                                                                 ATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAATGCGAAGTAGAAACCATTGCGCTGGAACATCTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG   <  SRR19973063.4417284/150‑1 (MQ=60)
                                                                                                                                                  TTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTGA  <  SRR19973063.1335338/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
AAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTGA  >  CP009273/207866‑208161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑