Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 208,013 G→T 100% M616I (ATG→ATT)  ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273208,0130GT98.1% 841.1 / 4.1 264M616I (ATG→ATT) ldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (128/130);  total (133/131)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 268 total aligned reads displayed.

ATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATG  >  CP009273/207868‑208157
                                                                                                                                                 |                                                                                                                                                
  atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCa                                                                                                                                              <  2:1880892/148‑1 (MQ=255)
  atGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCa                                                                                                                                              <  2:3768734/148‑1 (MQ=255)
   tGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCAt                                                                                                                                             >  2:3615579/1‑148 (MQ=255)
    gCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATg                                                                                                                                            >  1:1038950/1‑148 (MQ=255)
    gCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATg                                                                                                                                            >  1:2946946/1‑148 (MQ=255)
    gCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATg                                                                                                                                            <  2:1341074/148‑1 (MQ=255)
       atccGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGAct                                                                                                                                         >  2:3171653/3‑147 (MQ=255)
       aCCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACg                                                                                                                                         >  2:206258/1‑148 (MQ=255)
       aCCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACg                                                                                                                                         >  2:2133642/1‑148 (MQ=255)
         ccGATCTCTATGCAGAATATCCCGATTTCTACCGCAATATGCGTATTCATGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGAACTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGcc                                                                                                                                       >  1:2255966/1‑148 (MQ=255)
         ccGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGcc                                                                                                                                       >  2:3698012/1‑148 (MQ=255)
            aTCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCcaca                                                                                                                                    >  1:1323651/1‑148 (MQ=255)
            aTCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCcaca                                                                                                                                    >  1:3561900/1‑148 (MQ=255)
            aTCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCcaca                                                                                                                                    >  1:2418866/1‑148 (MQ=255)
             tctcTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACAt                                                                                                                                   >  2:1416329/1‑148 (MQ=255)
              ctctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATc                                                                                                                                  <  1:3649858/148‑1 (MQ=255)
               tctATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCa                                                                                                                                 >  2:3777972/1‑148 (MQ=255)
                 tATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAgg                                                                                                                               <  2:2479675/148‑1 (MQ=255)
                     cAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATg                                                                                                                           >  2:3506725/1‑148 (MQ=255)
                     cAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATg                                                                                                                           <  1:3084781/148‑1 (MQ=255)
                       gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGc                                                                                                                         >  2:482187/1‑148 (MQ=255)
                       gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGc                                                                                                                         >  2:1323463/1‑148 (MQ=255)
                       gaagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGc                                                                                                                         >  2:1320499/1‑148 (MQ=255)
                        aagaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCa                                                                                                                        >  2:1749044/1‑148 (MQ=255)
                          gaTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAAc                                                                                                                      >  1:2163073/1‑148 (MQ=255)
                           aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACCAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACg                                                                                                                     >  1:3770610/1‑148 (MQ=255)
                           aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACg                                                                                                                     <  1:1210611/148‑1 (MQ=255)
                           aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACg                                                                                                                     >  1:1093223/1‑148 (MQ=255)
                           aTCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACg                                                                                                                     <  2:2786611/148‑1 (MQ=255)
                            tCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGa                                                                                                                    >  2:1501018/1‑148 (MQ=255)
                              ccGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACa                                                                                                                  <  2:161720/148‑1 (MQ=255)
                               cGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACaa                                                                                                                 <  1:2991981/148‑1 (MQ=255)
                                 aTTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAAt                                                                                                               <  2:993890/148‑1 (MQ=255)
                                   ttCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTa                                                                                                             >  2:3005458/1‑148 (MQ=255)
                                   ttCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTa                                                                                                             >  1:44784/1‑148 (MQ=255)
                                   ttCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTa                                                                                                             >  2:3664568/1‑148 (MQ=255)
                                       aCCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAgg                                                                                                         <  1:499149/148‑1 (MQ=255)
                                           cAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGaa                                                                                                     <  1:1548102/148‑1 (MQ=255)
                                             atatGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGt                                                                                                   >  1:913826/1‑148 (MQ=255)
                                                     aTTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCa                                                                                           >  2:515980/1‑148 (MQ=255)
                                                      ttCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGATGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAt                                                                                          <  1:2505308/148‑1 (MQ=255)
                                                      ttCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAt                                                                                          >  1:2617765/1‑148 (MQ=255)
                                                          ggATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgcg                                                                                      <  1:1600939/148‑1 (MQ=255)
                                                          ggATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATATTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGTAACCATTgcg                                                                                      <  2:2525208/148‑1 (MQ=255)
                                                           gATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTgcgc                                                                                     >  1:1156284/1‑148 (MQ=255)
                                                             tCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTg                                                                                   <  1:2216536/148‑1 (MQ=255)
                                                                 gCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGaac                                                                               >  1:1016146/1‑148 (MQ=255)
                                                                    caAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGaacaac                                                                            >  2:2333496/1‑148 (MQ=255)
                                                                    caAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGaacaac                                                                            <  1:1416329/148‑1 (MQ=255)
                                                                    caAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGaacaac                                                                            >  2:2509417/1‑148 (MQ=255)
                                                                       gggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTgg                                                                         >  2:930621/1‑148 (MQ=255)
                                                                        ggATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGt                                                                        >  2:1679122/1‑148 (MQ=255)
                                                                               tAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTaga                                                                 <  2:3749535/148‑1 (MQ=255)
                                                                                aaGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTagag                                                                <  1:3048141/148‑1 (MQ=255)
                                                                                 aGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGt                                                               >  1:925059/1‑148 (MQ=255)
                                                                                   cTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTAt                                                             <  1:1892981/148‑1 (MQ=255)
                                                                                       ttCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGc                                                         >  1:720568/1‑148 (MQ=255)
                                                                                       ttCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGc                                                         >  1:3726197/1‑148 (MQ=255)
                                                                                         cGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCaa                                                       <  2:1093223/148‑1 (MQ=255)
                                                                                           tAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAat                                                     >  1:2385797/1‑148 (MQ=255)
                                                                                           tAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAat                                                     <  1:782646/148‑1 (MQ=255)
                                                                                               cacGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGa                                                 <  1:3784890/148‑1 (MQ=255)
                                                                                                    tCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTg                                            >  1:2662559/1‑148 (MQ=255)
                                                                                                    tCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTg                                            >  1:2029880/1‑148 (MQ=255)
                                                                                                     cTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGc                                           <  1:3754309/148‑1 (MQ=255)
                                                                                                      ttCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGcc                                          <  2:1540391/148‑1 (MQ=255)
                                                                                                      ttCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACTCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGcc                                          >  1:2831651/1‑148 (MQ=255)
                                                                                                         ccGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTa                                       <  1:3720185/148‑1 (MQ=255)
                                                                                                          cGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAt                                      <  2:2093944/148‑1 (MQ=255)
                                                                                                           tgTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATc                                     <  1:3366472/147‑1 (MQ=255)
                                                                                                             tttGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCa                                   <  2:2370074/148‑1 (MQ=255)
                                                                                                                 aTGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCgg                               >  1:3468661/1‑148 (MQ=255)
                                                                                                                 aTGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCgg                               >  2:2071151/1‑148 (MQ=255)
                                                                                                                 aTGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCgg                               >  2:2706359/1‑148 (MQ=255)
                                                                                                                   gTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACACCTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGc                             >  2:641614/1‑148 (MQ=255)
                                                                                                                   gTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGc                             >  2:1613009/1‑148 (MQ=255)
                                                                                                                      gCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTa                          >  1:649210/1‑148 (MQ=255)
                                                                                                                             ttCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGt                   <  2:1040049/148‑1 (MQ=255)
                                                                                                                             ttCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGt                   <  2:3221150/148‑1 (MQ=255)
                                                                                                                             ttCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATCAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGt                   <  2:2946946/148‑1 (MQ=255)
                                                                                                                               cGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTg                 <  2:3092103/148‑1 (MQ=255)
                                                                                                                                gATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGa                <  1:1954723/148‑1 (MQ=255)
                                                                                                                                 aTACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGAt               >  2:2380803/1‑148 (MQ=255)
                                                                                                                                  tACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATg              >  1:274787/1‑148 (MQ=255)
                                                                                                                                  tACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATg              >  1:1246121/1‑148 (MQ=255)
                                                                                                                                   aCTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc             <  2:853546/148‑1 (MQ=255)
                                                                                                                                   aCTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc             <  1:943168/148‑1 (MQ=255)
                                                                                                                                   aCTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc             <  2:329267/148‑1 (MQ=255)
                                                                                                                                   aCTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc             <  2:1131167/148‑1 (MQ=255)
                                                                                                                                   aCTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGc             <  2:2255747/148‑1 (MQ=255)
                                                                                                                                    cTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGcc            >  2:1069845/1‑148 (MQ=255)
                                                                                                                                     tttGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCt           <  2:3303411/148‑1 (MQ=255)
                                                                                                                                     tttGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCt           <  1:3157148/148‑1 (MQ=255)
                                                                                                                                       tGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGAAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCAGTTGATGCCTgg         >  1:3803947/1‑148 (MQ=255)
                                                                                                                                          cGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGgaga      <  1:858319/148‑1 (MQ=255)
                                                                                                                                           ggAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGaa     <  2:3561900/148‑1 (MQ=255)
                                                                                                                                           ggAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGaa     <  1:1749044/148‑1 (MQ=255)
                                                                                                                                           ggAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGaa     <  1:2133642/148‑1 (MQ=255)
                                                                                                                                             agaTTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAAt   >  2:1968351/1‑148 (MQ=255)
                                                                                                                                             agaTTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAAt   >  2:2175498/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATG  >  CP009273/207868‑208157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG  >  CP009273/207864‑208160
                                                                                                                                                     |                                                                                                                                                   
AAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATT                                                                                                                                                     >  SRR19973069.331512/1‑150 (MQ=60)
AAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATT                                                                                                                                                     >  SRR19973069.331509/1‑150 (MQ=60)
AAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATT                                                                                                                                                     <  SRR19973069.3249037/150‑1 (MQ=60)
AAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATT                                                                                                                                                     >  SRR19973069.2270986/1‑150 (MQ=60)
AAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATT                                                                                                                                                     <  SRR19973069.1228390/150‑1 (MQ=60)
 AAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTA                                                                                                                                                    >  SRR19973069.993809/1‑150 (MQ=60)
 AAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTA                                                                                                                                                    >  SRR19973069.182806/1‑150 (MQ=60)
  AAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTAT                                                                                                                                                   <  SRR19973069.3768334/150‑1 (MQ=60)
  AAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTAT                                                                                                                                                   >  SRR19973069.3016545/1‑150 (MQ=60)
    ATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCA                                                                                                                                                 >  SRR19973069.123788/1‑150 (MQ=60)
    ATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCA                                                                                                                                                 >  SRR19973069.123825/1‑150 (MQ=60)
     TATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCAT                                                                                                                                                <  SRR19973069.1634129/150‑1 (MQ=60)
      ATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGGTTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATG                                                                                                                                               >  SRR19973069.1047277/1‑150 (MQ=60)
      ATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATG                                                                                                                                               >  SRR19973069.1045936/1‑150 (MQ=60)
      ATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATG                                                                                                                                               >  SRR19973069.2966504/1‑150 (MQ=60)
      ATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATG                                                                                                                                               <  SRR19973069.179788/150‑1 (MQ=60)
      ATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATG                                                                                                                                               >  SRR19973069.1046168/1‑150 (MQ=60)
       TGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGA                                                                                                                                              >  SRR19973069.859528/1‑150 (MQ=60)
         CTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACG                                                                                                                                            >  SRR19973069.1139055/1‑150 (MQ=60)
           ACCCGATCTCTATGCAGAATATCCCGATTTCTACCGCAATATGCGTATTCATGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGAACTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCC                                                                                                                                          >  SRR19973069.2271208/1‑150 (MQ=60)
           ACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCC                                                                                                                                          <  SRR19973069.2784139/150‑1 (MQ=60)
            CCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCA                                                                                                                                         >  SRR19973069.2243874/1‑150 (MQ=60)
              CGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACA                                                                                                                                       >  SRR19973069.1332749/1‑150 (MQ=60)
              CGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACA                                                                                                                                       >  SRR19973069.1332741/1‑150 (MQ=60)
              CGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACA                                                                                                                                       >  SRR19973069.3585088/1‑150 (MQ=60)
              CGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACA                                                                                                                                       >  SRR19973069.2435205/1‑150 (MQ=60)
               GATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACAT                                                                                                                                      >  SRR19973069.3757420/1‑150 (MQ=60)
               GATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACAT                                                                                                                                      >  SRR19973069.870370/1‑150 (MQ=60)
               GATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACAT                                                                                                                                      >  SRR19973069.3667598/1‑150 (MQ=60)
               GATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACAT                                                                                                                                      >  SRR19973069.3081647/1‑150 (MQ=60)
                 TCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCA                                                                                                                                    <  SRR19973069.1507919/150‑1 (MQ=60)
                  CTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAG                                                                                                                                   <  SRR19973069.2575958/150‑1 (MQ=60)
                  CTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAG                                                                                                                                   <  SRR19973069.3673765/150‑1 (MQ=60)
                      ATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCAT                                                                                                                               <  SRR19973069.2434602/150‑1 (MQ=60)
                        GCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGG                                                                                                                             <  SRR19973069.3631130/150‑1 (MQ=60)
                         CAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGC                                                                                                                            <  SRR19973069.3105120/150‑1 (MQ=60)
                            AAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAAC                                                                                                                         >  SRR19973069.2177869/1‑150 (MQ=60)
                            AAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAAC                                                                                                                         <  SRR19973069.3269881/150‑1 (MQ=60)
                             AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACCAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                        >  SRR19973069.3795105/1‑150 (MQ=60)
                             AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                        >  SRR19973069.1085927/1‑150 (MQ=60)
                             AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                        >  SRR19973069.1100858/1‑150 (MQ=60)
                             AGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACG                                                                                                                        >  SRR19973069.3795245/1‑150 (MQ=60)
                               ATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGAC                                                                                                                      <  SRR19973069.1218918/150‑1 (MQ=60)
                               ATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGAC                                                                                                                      <  SRR19973069.886323/150‑1 (MQ=60)
                                TCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACA                                                                                                                     >  SRR19973069.3345122/1‑150 (MQ=60)
                                TCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACA                                                                                                                     <  SRR19973069.2653814/150‑1 (MQ=60)
                                   CGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAAT                                                                                                                  <  SRR19973069.3011848/150‑1 (MQ=60)
                                    GATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCACCGACAAATT                                                                                                                 >  SRR19973069.2854667/1‑150 (MQ=60)
                                    GATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATT                                                                                                                 >  SRR19973069.574762/1‑150 (MQ=60)
                                    GATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATT                                                                                                                 >  SRR19973069.2496958/1‑150 (MQ=60)
                                     ATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTA                                                                                                                >  SRR19973069.3112479/1‑150 (MQ=60)
                                     ATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTA                                                                                                                >  SRR19973069.1194170/1‑150 (MQ=60)
                                     ATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTA                                                                                                                >  SRR19973069.45362/1‑150 (MQ=60)
                                        TCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAG                                                                                                             <  SRR19973069.663264/150‑1 (MQ=60)
                                          TACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGC                                                                                                           <  SRR19973069.3103709/150‑1 (MQ=60)
                                          TACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGC                                                                                                           >  SRR19973069.631516/1‑150 (MQ=60)
                                           ACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCG                                                                                                          <  SRR19973069.502731/150‑1 (MQ=60)
                                               CAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGT                                                                                                      >  SRR19973069.920070/1‑150 (MQ=60)
                                               CAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGT                                                                                                      <  SRR19973069.1558691/150‑1 (MQ=60)
                                                AATATGCGTTTTCAGGATCTGGCACAATGGATACATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCTCATCAGGCATGGCAACGACAAATTAAAGGCGAAGTA                                                                                                     <  SRR19973069.1961855/150‑1 (MQ=60)
                                                AATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTA                                                                                                     <  SRR19973069.15679/150‑1 (MQ=60)
                                                   ATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAA                                                                                                  >  SRR19973069.269903/1‑150 (MQ=60)
                                                        TATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCAT                                                                                             >  SRR19973069.2635267/1‑150 (MQ=60)
                                                          TTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGATGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTG                                                                                           <  SRR19973069.2522022/150‑1 (MQ=60)
                                                             AGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGC                                                                                        >  SRR19973069.1164341/1‑150 (MQ=60)
                                                              GGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCT                                                                                       <  SRR19973069.1611729/150‑1 (MQ=60)
                                                               GATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTG                                                                                      >  SRR19973069.786199/1‑150 (MQ=60)
                                                               GATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTG                                                                                      >  SRR19973069.2745571/1‑150 (MQ=60)
                                                                 TCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGA                                                                                    <  SRR19973069.2231540/150‑1 (MQ=60)
                                                                 TCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGA                                                                                    <  SRR19973069.2231546/150‑1 (MQ=60)
                                                                   TGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAAC                                                                                  >  SRR19973069.1023156/1‑150 (MQ=60)
                                                                        CAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTG                                                                             <  SRR19973069.1426211/150‑1 (MQ=60)
                                                                           GGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTC                                                                          >  SRR19973069.2012597/1‑150 (MQ=60)
                                                                                CCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAG                                                                     >  SRR19973069.2171478/1‑150 (MQ=60)
                                                                                   TAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGT                                                                  >  SRR19973069.931395/1‑150 (MQ=60)
                                                                                   TAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGT                                                                  >  SRR19973069.931173/1‑150 (MQ=60)
                                                                                   TAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGT                                                                  >  SRR19973069.931355/1‑150 (MQ=60)
                                                                                    AAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTA                                                                 <  SRR19973069.3068328/150‑1 (MQ=60)
                                                                                     AGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTAT                                                                >  SRR19973069.2886363/1‑150 (MQ=60)
                                                                                       CTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCG                                                              <  SRR19973069.2162632/150‑1 (MQ=60)
                                                                                       CTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCG                                                              <  SRR19973069.3722181/150‑1 (MQ=60)
                                                                                       CTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCG                                                              <  SRR19973069.1906100/150‑1 (MQ=60)
                                                                                         GATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGC                                                            >  SRR19973069.3750416/1‑150 (MQ=60)
                                                                                         GATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGC                                                            >  SRR19973069.3750506/1‑150 (MQ=60)
                                                                                         GATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGC                                                            >  SRR19973069.725648/1‑150 (MQ=60)
                                                                                             CGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAAT                                                        >  SRR19973069.2401968/1‑150 (MQ=60)
                                                                                               TAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATAT                                                      <  SRR19973069.1255337/150‑1 (MQ=60)
                                                                                               TAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATAT                                                      <  SRR19973069.788196/150‑1 (MQ=60)
                                                                                               TAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATAT                                                      <  SRR19973069.789094/150‑1 (MQ=60)
                                                                                                   CACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATC                                                  <  SRR19973069.3809448/150‑1 (MQ=60)
                                                                                                    ACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCC                                                 <  SRR19973069.3565430/150‑1 (MQ=60)
                                                                                                    ACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCC                                                 <  SRR19973069.949216/150‑1 (MQ=60)
                                                                                                     CGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCT                                                <  SRR19973069.3675160/150‑1 (MQ=60)
                                                                                                      GATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTG                                               <  SRR19973069.2281530/150‑1 (MQ=60)
                                                                                                      GATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTG                                               >  SRR19973069.2043982/1‑150 (MQ=60)
                                                                                                      GATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTG                                               >  SRR19973069.2680408/1‑150 (MQ=60)
                                                                                                      GATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTG                                               <  SRR19973069.393495/150‑1 (MQ=60)
                                                                                                      GATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTG                                               >  SRR19973069.2214122/1‑150 (MQ=60)
                                                                                                       ATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGC                                              >  SRR19973069.2590627/1‑150 (MQ=60)
                                                                                                        TCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACTCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGTTAGAGTATCGGCAAATATGATCCTGCC                                             >  SRR19973069.2850359/1‑150 (MQ=60)
                                                                                                         CTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCT                                            <  SRR19973069.3407747/150‑1 (MQ=60)
                                                                                                         CTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCT                                            <  SRR19973069.3778735/150‑1 (MQ=60)
                                                                                                         CTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCT                                            <  SRR19973069.430406/150‑1 (MQ=60)
                                                                                                          TTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTT                                           <  SRR19973069.280540/150‑1 (MQ=60)
                                                                                                            CCCGGTTTGATGTTGCGGGCATTCGATACTGTGCCGGTGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTAT                                         <  SRR19973069.2287203/150‑1 (MQ=60)
                                                                                                             CCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATC                                        <  SRR19973069.3744472/150‑1 (MQ=60)
                                                                                                              CGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCC                                       >  SRR19973069.3039010/1‑150 (MQ=60)
                                                                                                               TGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCA                                      <  SRR19973069.3388488/150‑1 (MQ=60)
                                                                                                               GGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCA                                      >  SRR19973069.1131297/1‑150 (MQ=60)
                                                                                                                  TTGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCG                                   <  SRR19973069.2476564/150‑1 (MQ=60)
                                                                                                                   TGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGG                                  >  SRR19973069.299668/1‑150 (MQ=60)
                                                                                                                   TGATGTTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGG                                  >  SRR19973069.3491098/1‑150 (MQ=60)
                                                                                                                        TTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTA                             >  SRR19973069.653512/1‑150 (MQ=60)
                                                                                                                        TTGCGGGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTA                             >  SRR19973069.653693/1‑150 (MQ=60)
                                                                                                                             GGCATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTTGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCT                        <  SRR19973069.1380845/150‑1 (MQ=60)
                                                                                                                               CATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGT                      >  SRR19973069.467615/1‑150 (MQ=60)
                                                                                                                               CATTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGT                      <  SRR19973069.3763042/150‑1 (MQ=60)
                                                                                                                                 TTCGATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTG                    >  SRR19973069.1966272/1‑150 (MQ=60)
                                                                                                                                    GATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATG                 <  SRR19973069.1968249/150‑1 (MQ=60)
                                                                                                                                    GATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATG                 >  SRR19973069.276722/1‑150 (MQ=60)
                                                                                                                                    GATACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATG                 >  SRR19973069.1254576/1‑150 (MQ=60)
                                                                                                                                       ACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCT              <  SRR19973069.949530/150‑1 (MQ=60)
                                                                                                                                       ACTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCT              >  SRR19973069.3408435/1‑150 (MQ=60)
                                                                                                                                        CTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTG             <  SRR19973069.3791373/150‑1 (MQ=60)
                                                                                                                                        CTTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTG             <  SRR19973069.3791028/150‑1 (MQ=60)
                                                                                                                                         TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGG            <  SRR19973069.1581789/150‑1 (MQ=60)
                                                                                                                                         TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGG            >  SRR19973069.3827555/1‑150 (MQ=60)
                                                                                                                                         TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGG            >  SRR19973069.1582363/1‑150 (MQ=60)
                                                                                                                                         TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGG            <  SRR19973069.3177759/150‑1 (MQ=60)
                                                                                                                                         TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGG            >  SRR19973069.3828521/1‑150 (MQ=60)
                                                                                                                                         TTTGCCGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGAAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCAGTTGATGCCTGG            >  SRR19973069.3828580/1‑150 (MQ=60)
                                                                                                                                              CGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAGGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAA       >  SRR19973069.1764712/1‑150 (MQ=60)
                                                                                                                                              CGGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAA       <  SRR19973069.864325/150‑1 (MQ=60)
                                                                                                                                               GGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAAT      <  SRR19973069.1761355/150‑1 (MQ=60)
                                                                                                                                               GGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAAT      <  SRR19973069.207906/150‑1 (MQ=60)
                                                                                                                                               GGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAAT      <  SRR19973069.208062/150‑1 (MQ=60)
                                                                                                                                               GGAGATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAAT      <  SRR19973069.2148309/150‑1 (MQ=60)
                                                                                                                                                  GATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCT   >  SRR19973069.3577458/1‑150 (MQ=60)
                                                                                                                                                  GATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCT   >  SRR19973069.3765218/1‑150 (MQ=60)
                                                                                                                                                  GATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCT   >  SRR19973069.1671614/1‑150 (MQ=60)
                                                                                                                                                   ATTATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG  >  SRR19973069.762668/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTTTGATGTTGCGGGCATTCGATACTTTGCCGGAGATGATCATGACGCCACATCAGGCATGGCAACGACAAATTAAAGGCGAAGTAGAAACCATTGCGCTGGAACAACTGGTCGGTAGAGTATCGGCAAATATGATCCTGCCTTATCCACCGGGCGTACCGCTGTTGATGCCTGGAGAAATGCTG  >  CP009273/207864‑208160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑